Changes over time in the adaptation of barley releases in north-eastern Spain

The objective of this study was to characterize grain yield and adaptation of barley cultivars released in recent decades in north-eastern Spain. Ten six-rowed and 10 two-rowed barley genotypes were grown in eight environments. Mean yields varied among environments between 1.7 and 5.8 tha^?1. New releases showed higher mean yields and were specifically adapted to nonlimiting growing conditions. The average genetic advances were estimated as 40.7 and 32.9 kg ha^?1 years^?1 for two- and six-rowed genotypes, respectively, in the four most productive environments. Old genotypes, with lower overall grain yields, were specifically adapted to the poorer sites. A negative genetic gain, estimated as -14.8kg ha^?1 years^?1. was reorded for six-rowed culiivars in the four poorest sites. No significant association was observed between heading date and year of release. Variations in carbon isotope discrimination (Δ) were examined to assess whether changes in adaptation could be partially explained by shifts in the genoiypic transpiration efficiency (TE) over time, in the most favourable environments, new releases showed the highest grain yields and carbon isotope discrimination values (Δ). By contrast, older cultivars had constitutively? lower Δs and therefore higher TEs. These results suggest a genetic association between low TE and yield potential and specific adaptation to stress-free conditions so that selection for yield under favourable conditions could have resulted in an indirect downward genetic shift in the TE of modern cultivars.     

Molecular assessment of genetic diversity in winter barley and its use in breeding

Summary During the last decades extensive progress has been achieved in winter barley breeding with respect to both, yield and resistance to fungal and viral diseases. This progress is mainly due to the efficient use of the genetic diversity present within high yielding adapted cultivars and – with respect to resistance – to the extensive evaluation of genetic resources followed by genetic analyses and introgression of respective genes by sexual recombination. Detailed knowledge on genetic diversity present on the molecular level regarding specific traits as well as on the whole genome level may enhance barley breeding today by facilitating efficient selection of parental lines and marker assisted selection procedures. In the present paper the state of the art with respect to virus diseases, i.e. Barley mild mosaic virus, Barley yellow mosaic virus, and Barley yellow dwarf virus is briefly reviewed and first results on a project aiming on a genome wide estimation of genetic diversity which in combination with data on yield and additional agronomic traits may facilitate the detection of marker trait associations and a more efficient selection of parental genotypes are presented. By field tests of 49 two-rowed and 64 six-rowed winter barley cultivars the genetic gain in yield for the period 1970–2003 was estimated at 54.6 kg ha⁻¹ year⁻¹ (r² = 0.567) for the six-rowed cultivars and at 37.5 kg ha⁻¹ year⁻¹ (r² = 0.621) for the two-rowed cultivars. Analysis of 30 SSRs revealed a non-homogenous allele distribution between two and six-rowed cultivars and changes of allele frequencies in relation to the time of release. By PCoA a separation between two and six-rowed cultivars was observed but no clear cut differentiation in relation to the time of release. In the two-rowed cultivars an increase in genetic diversity (DI) from older to newly released cultivars was detected.     

Genetic improvement of yield responsiveness to nitrogen fertilization and its physiological determinants in barley

Traditionally, barley in Argentina has been cultivated in low-yielding environments. A study was conducted to test whether breeding for improved performance under these conditions would have also improved the responsiveness to nitrogen availability. Four cultivars of two-rowed malting barley (released in 1944, 1960,1982 and 1998) were grown under 4 rates of nitrogen fertilizer at sowing (20, 50, 110and 160 kgN ha^-1). All cultivars increased their yield with the increase in soil nitrogen. But yield of modern cultivars responded more strongly than yield of old ones. For modern cultivars, increase in grain yield was of 12 ± 0.6 kgha^-1 for each 10 kg ha^-1 of increase in the mean yield (environmental index). Absolute values of genetic gain were related to nitrogen availability: 1.59, 2.58, 4.52 and 4.29 g m^-2 year^-1 for the N₂₀, N₅₀, N₁₁₀ and N₁₆₀ treatments, respectively. Grain yield was associated with grain number m^-2, which was dependent on spikes m^-2 and grains spike^-1. Total biomass at maturity also explained the changes in yield. It is concluded that selection under stress conditions was, in this case, beneficial to identify cultivars with high yields under a wide range of nitrogen availabilities. This revised version was published online in July 2006 with corrections to the Cover Date.     

2种穗型小麦品种若干性状差异的比较

为了探明胶东地区适宜的小麦品种类型与育种方向,通过11个不同的小麦基因型,采用t测验法对2种穗型小麦品种的若干性状差异进行了研究。结果表明:大穗型品种的单茎草重、茎质系数、穗粒数、千粒重、穗粒重比多穗型品种高0.958 g、0.011 g/cm、5.354粒、5.460 g、0.504 g,均达到极显著水平;多穗型品种的单位面积穗数、经济系数和群体产量比大穗型品种高289.847万/hm2、0.027、859 kg/hm2,均达到极显著水平。在胶东地区生态条件下,应以分蘖力中等、茎秆粗壮、成穗率高、产量三因素协调的多穗型品种为主攻方向。     

Genetic improvement in wheat yield and associated traits. A re-examination of previous results and the latest trends

Two field experiments were carried out with seven wheat cultivars (three of them, including a commercial hybrid, released during the last 10 years) representing different eras of plant breeding, to evaluate genetic improvement over the last century in grain yield, height, biomass, harvest index and grain yield components. Plots were fertilized and irrigated, and lodging and diseases were prevented. Main culm height was negatively correlated with the year of release of the cultivars, probably as a consequence of selection for increased lodging resistance. There was no significant association between total above-ground biomass and year of release of the cultivars. On the other hand, grain yield increased as newer cultivars were released. Results indicate that during recent years harvest index has been kept as the main attribute responsible for increases in grain yield. In general, number of grains/m² was associated with increases in grain yield during the century. However, the newest cultivars showed an increased grain weight. In both growing seasons, cultivars released before 1980 showed a trend towards reduced grain weight, but cultivars released after 1987 had a similar number of grains per m² with a higher grain weight than their predecessors. This was probably because the most modern cultivars have a longer grain-filling duration with a similar length of growth cycle.     

The impact of variation in grain number and individual grain weight on winter wheat yield in the high yield potential environment of Ireland

Previous studies from regions that produce high proportions of global winter wheat have highlighted that difference in sink size influences the majority of variations in winter wheat yield. However, the potential for source limitation due to environmental differences in regions that consistently produce a large sink capacity (i.e. >20,000 grains/m²), such as Ireland, have not been widely studied. The aim of this study was to characterise the variation in growth pattern and yield components that contribute to variations in grain yield in regions of high yield potential, and to identify the periods of development that are most likely to influence yield in these regions. Monitor crops of winter wheat were grown at three sites with contrasting latitudes on the island of Ireland, during three growing seasons (2013–2015). Crops were assessed regularly for measurements of crop growth and development, including biomass accumulation, canopy development and light interception. Grain yield ranged between 10.7–15.8 t/ha at 15% moisture content, with a grand mean of 12.7 t/ha. Results indicated that variations in grains/m² had a larger effect on winter wheat yield than variations in individual grain weight. Variability in grains/m² was influenced by changes in spikes/m² more than the number of grains/spike. While spikes/m² at harvest was significantly related to the number of shoots/m² at GS59, no significant relationship was observed between the shoots/m² at the time of maximum tillers/plant and spikes/m² at harvest. Furthermore, a significantly negative linear relationship was observed between shoots/m² at the time of maximum tillers/plant and grains/spike. Therefore, high rates of tillering were not beneficial to yield formation in the majority of crops monitored. A strong effect of individual grain weight was observed at one site of the nine evaluated in the study, indicating that a partial source limitation of yield is possible in certain Irish environmental conditions. However, variations in grain yield of crops of winter wheat grown at different locations in Ireland in different seasons were primarily driven by variations in grain number, and therefore were generally sink-limited.     

An alternative approach to breeding for high diastatic power in barley

Summary Several lines from the cross Akka x Feebar were observed to have -amylase activity considerably in excess of either parent. It is suggested that, from crosses between two-rowed and six-rowed varieties, two-rowed genotypes with enhanced levels of grain nitrogen and -amylase activity may be obtained. These can be successfully exploited in a breeding programme to produce barley varities with high diastic power.     

Recurrent selection for grain yield in early generations of two barley populations

Summary Two spring barley composites, one based on eight West-European two-rowed cultivars (A) and one, the predominantly six-rowed composite XXI, based on several thousands of barley cultivars (B), formed the starting point of a recurrent selection procedure. The aim was to study whether recurrent selection in early generations is an effective procedure to improve barley populations for agronomic characteristics, especially grain yield.After two cycles of recurrent selection in the two populations A and B separately, and consisting of single plant selection followed by line selection, one cycle of recurrent selection was applied to the population produced by intercrossing the selected A and selected B lines.The selection for grain yield in the F2 single plant stages and the F3 line stages was carried out by selecting the plants or lines with the largest mean head weight provided that they did not tiller too poorly. Also some selection was applied against extremes in heading date and against tall plants.After two cycles of recurrent selection the grain yield, tested over two years and two locations, had increased with 16.5% in population A and with 27.0% in population B. Both the single plant selection and the line selection had contributed significantly to this response.The population created by crossing the selected A with the selected B lines showed a significant reduction in yield compared to the mean yield of the A and B lines constituting this population. Single plant selection, followed by line selection did raise the yield level 5.7% above that of the mean of these A and B lines. Several lines produced from this intercross combined an excellent grain yield with outstanding resistance to barley leaf rust and powdery mildew. However, these lines like the entire intercross population suffered from susceptibility to lodging, a characteristic derived from population B.In five experimental situations mixtures of genotypes were compared with the mean of the monocultures of the constituting genotypes. The mixture yielded always more, the average mixture effect being 4.5%.     

Genetic improvement of grain yield and associated traits in the southern China winter wheat region: 1949 to 2000

To understand the genetic gains of grain yield in the Southern China Winter Wheat Region (SCWWR), two yield potential trials, i.e., YPT 1 including 11 leading cultivars from the Middle and Low Yangtze Valley (Zone III) and YPT 2 including 15 leading cultivars from the Southwestern China Region (Zone IV) from 1949 to 2000, were conduced during the 2001–2003 cropping seasons. A completely randomized block design of three replicates was employed with controlled field environments. Molecular markers were used to detect the presence of dwarfing genes and the 1B/1R translocation. Results showed that average annual genetic gain was 0.31% (P < 0.05) or 13.96 kg/ha/year and 0.74% (P < 0.01) or 40.80 kg/ha/year in Zones III and IV, respectively. In YPT 1, changes of all other traits were not significant, but plant height was significantly reduced. In YPT 2, the genetic improvement of grain yield was primarily attributed to the increased thousand kernel weight (TKW) (0.65%, P < 0.01) and kernel weight/spike (0.87%, P < 0.01), reduced plant height and increased harvest index (HI). The dwarfing gene Rht 8 was most frequently present (46.1%), Rht-B1b was observed in three genotypes in Zone III, and Rht-D1b was present in only one genotype in Zone IV. The 1B/1R translocation was present in four genotypes. Utilization of Italian germplasm and development of landmark cultivar Fan 7 were the key factors for grain yield improvement in SCWWR. The future challenge of wheat breeding in this region is to continue improving grain yield and disease resistance, and to develop cultivars suitable for the reduced tillage of wheat/rice double cropping. Utilization of Mexican germplasm could provide opportunities for future yield improvement.     

Genetic progress of oats in Italy

The genetic gain of oats in Italy over the past 40 years was evaluated using 14 cultivars belonging to three different groups: (i) five cultivars derived from local populations at the beginning of the oat breeding in Italy; (ii) four foreign cultivars, introduced in Italy in the 1980s; (iii) five modern cultivars, selected through specific breeding programmes. The genotypes were tested for 2 years in three locations characterised by different climatic conditions and agricultural practices; furthermore in one location in Southern Italy, irrigated and rainfed plots were compared. Genetic gain was estimated equal to 3.6 g m⁻² y⁻¹ based on eight environments. The performance of the most recent cultivars was high under both irrigated and rainfed conditions, showing a good adaptation of these genotypes to both fertile and drought prone environments. Finally, yield increase in oats was found to be negatively correlated with plant height and positively correlated with test weight and seed weight.     

Quantitative trait loci for grain yield and yield components in a cross between a six-rowed and a two-rowed barley

Summary The positions of quantitative trait loci (QTL) for yield and yield components were estimated using a 85-point linkage map and phenotype data from a F₁-derived doubled haploid (DH) population of barley. Yield and its components were recorded in two growing seasons. Highly significant QTL effects were found for all traits at several sites in the genome. A major portion of the QTL was found on chromosome 2. The effect of the alleles in locus v on thousand grain weight and kernels per ear explained 70–80% of the genetic variation in the traits. QTL × year interaction was found for grain yield. Several different QTL were found within the two-rowed DH lines compared to those found in the six-rowed DH lines. Epistasis between locus v and several loci for yield and yield components indicates that genes are expressed differently in the two ear types. This may explain the difficulties of selecting high yielding lines from crosses between two-rowed and six-rowed barley.Abbreviations DH doubled haploid - QTL quantitative trait locus/loci - RAPD random amplified polymorphic DNA - RFLP restriction fragment length polymorphism - T. Prentice Tystofte Prentice - V. Gold Vogelsanger Gold     

Genetic improvement of agronomic and qualitative traits of spring barley

Five experiments, involving 24 genotypes, were carried out over three seasons to estimate the increase both in grain yield and other selected agronomic traits, and in potential malt extract of spring barley achieved by plant breeders during a period of over 160 years. In all experiments there was neither a chemical control of fungal diseases nor physical support of the tall, older genotypes to prevent yield losses from attack by fungi and lodging, respectively. In all experiments the most modern cultivars yielded more than the older ones. Significant breeding progress was also observed for plant height, resistance score, protein content and potential malt extract. The trend over time was generally of a curvilinear nature, showing no or only a slight increase till the 1950s and/or 1960s, and a greater rate of increase during the last 40 years of barley breeding. Using data from the official trials of cultivars and advanced breeding lines a linear increase in yield per year of 60 kg/ha since the 1950s was calculated for the low‐rainfall conditions of eastern Austria.     

Responses of Some Newly Developed Salt-tolerant Genotypes of Spring Wheat to Salt Stress: 1. Yield Components and Ion Distribution

The degree of salt tolerance of two newly developed genotypes of spring wheat, S24 and S36 was assessed with respect to their parents, LU26S (from Pakistan) and Kharchia (from India). These four lines along with a salt-tolerant genotype SARC-1 and two salt-sensitive cvs Potohar and Yecora Rojo were subjected to salinized sand culture containing 0, 125 or 250 mol m^?3 NaCl in full strength Hoagland's nutrient solution. S24 produced significantly greater grain yield and had greater 1000 seed weight and number of tillers per plant than those of the other cultivars /lines. S36 was not significantly different from its parents in seed yield and yield components. SARC-1 was the second highest in grain yield of all cultivars/lines, but it did not differ significantly from LU26S and Kharchia in 1000 seed weight and number of tillers per plant. The greater degree of salt tolerance of S24 could be related to its lower accumulation of Na⁺ in the leaves and maintenance of higher leaf K/Na ratios and K versus Na selectivity as compared to its parents. S36, which was as good as its parents in growth, also had lower Na⁺ and higher K/Na ratios and K versus Na selectivity in the leaves at the highest salt level than those in its parents. SARC-1 did not differ from LU26S and Kharchia in ionic content or K/Na ratios and K versus Na selectivities of both leaves and roots. Both the salt-sensitive cultivars, Potohar and Yecora Rojo, had significantly greater leaf Na⁺ and Cl^? concentrations and lower leaf K/Na ratios and K versus Na selectivities than all the salt-tolerant lines examined in this study. From this study it is evident that improvement in salt tolerance of spring wheat is possible through selection and breeding, and pattern of ion accumulation is not consistent among the salt-tolerant genotypes in relation to their degree of salt tolerance.     

Selection of wheat genotypes for biomass allocation to improve drought tolerance and carbon sequestration into soils

The biomass allocation pattern of plants to shoots and roots is a key in the cycle of elements such as carbon, water and nutrients with, for instance, the greatest allocations to roots fostering the transfer of atmospheric carbon to soils through photosynthesis. Several studies have investigated the root to shoot ratio (R:S) biomass of existing crops but variation within a crop species constitutes an important information gap for selecting genotypes aiming for increasing soil carbon stocks for climate change mitigation and food security. The objectives of this study were to evaluate agronomic performance and quantify biomass production and allocation between roots and shoots, in response to different soil water levels to select promising genotypes for breeding. Field and greenhouse experiments were carried out using 100 genotypes including wheat and Triticale under drought‐stressed and non‐stressed conditions. The experiments were set‐up using a 10 × 10 alpha lattice design with two replications under water stress and non‐stress conditions. The following phenotypic traits were collected: number of days to heading (DTH), number of productive tillers per plant (NPT), plant height (PH), days to maturity (DTM), spike length (SL), kernels per spike (KPS), thousand kernel weight (TKW), root biomass (RB), shoot biomass (SB), root to shoot ratio (R:S) and grain yield (GY). There was significant (p < 0.05) variation for grain yield and biomass production because of genotypic variation. The highest grain yield of 247.3 g/m² was recorded in the genotype LM52 and the least was in genotype Sossognon with 30 g/m². Shoot biomass ranged from 830 g/m² (genotype Arenza) to 437 g/m² (LM57), whilst root biomass ranged between 603 g/m² for Triticale and 140 g/m² for LM15 across testing sites and water regimes. Triticale also recorded the highest R:S of 1.2, whilst the least was 0.30 for wheat genotype LM18. Overall, drought stress reduced total biomass production by 35% and R:S by 14%. Genotypic variation existed for all measured traits useful for improving drought tolerance, whilst the calculated R:S values can improve accuracy in estimating C sequestration potential of wheat. Wheat genotypes LM26, LM47, BW140, LM70, LM48, BW152, LM75, BW162, LM71 and BW141 were selected for further development based on their high total biomass production, grain yield potential and genetic diversity under drought stress.     

Effect of Different Methods and Timings of Stand Establishment on Performance of Rainfed Lowland Rice Under 0–50 cm Water Depth

About one-third of the total rice is grown under rainfed lowland conditions, mostly m south and south-east Asia. Crop productivity in this ecosystem can be improved by adopting suitable management practices as drainage of excess water is not feasible in the catchment and coastal areas. Field experiments were conducted using a long-duration (165 days), photosensitive, semi-tall (150 cm) rice cultivar Utkalprabha established through direct sowing or transplanting on different dates under 0–50 cm water depth at Cuttack, India, during 1989–91. Direct sowing was done in lines in dry soil from 10 May onward using 400 seeds/m² and continued at 10 day intervals until June. Transplanting was done after accumulation of water in the field from July until 15 August with seedlings raised in nursery seed-beds with or without fertilizer application (100 kg N and 8.7 kg P and 16.7 kg K/ha) and tillers removed from the direct-sown crop. Seedling emergence varied significantly from 127–212/m, irrespective of sowing date and was dependent on rains received after sowing. However, the early sown crops in spite of poor germination, performed well due to better establishment and tiller production before water rose to higher depths in the field. There was a decreasing trend in grain yield, particularly when the sowing was delayed beyond end of May. The loss in yield with delayed sowing in June was due to poor crop stand which could not be compensated for by applying 50 % more seed (600/m²) and N fertilizer (60 kg N/ha). Removal of some of the tillers (100–130/m²) from crops sown on 30 May with 600 seeds/m² for planting on an equivalent plot area did not cause any adverse effect on the performance of mother crop. The anticipated shortfall in yield due to lower panicles/m² with clonal tiller separation was compensated for by the resulting increase in panicle weight. The performance of transplanted crops depended greatly on the water depth at or soon after planting. In 1990, planting on 15 July in 30 cm water depth helped in relatively better establishment and grain yield at par with sowing on 10 May. However, in 1991, when there was a sudden and rapid increase in water level to higher depths (50 cm) immediately after planting, the early planted crops produced only a negligible yield (0–1.2 t/ha). Highest yield was obtained from the crop planted with clonal tillers followed by that raised with fertilized and unfertilized nursery seedlings. Clonal tillers were taller (90 cm) and had more dry weight (1.78 g) compared with nursery seedlings (50–80 cm and 0.25–0.91 g). Therefore, the clonally propagated crop established well and acclimatized faster in the similar flooded environment, resulting in significantly higher grain yield particularly under late planted conditions. The results suggested early sowing by the end of May and transplanting with clonal tillers uprooted from the direct-sown crops for higher productivity of rice under uncontrolled excess water conditions.     

Performance of S2 Winter Barley Progenies from Original and Improved Populations Developed via Recurrent Selection

The effect of recurrent selection procedure for improving grain yield in autogamous cereals was evaluated in a six-rowed winter barley population. Gain from selection was estimated by field testing 90 random S2 lines from each of the C₀, C₁ and C₂ populations. Response to selection for grain yield was 0.78 t/ha from C₀ to C₁ and 1.09 t/ha from C₁ to C₂. Broad-sense heritability and genotypic variance for grain yield remained high in all cycles which suggests further gain from additional selection cycles. Yield increase was due to a higher number of seeds/m2. Significant differences among mean values were observed for heading date (189 days in C₀ and 182 days in C₂), whereas no variation was seen for plant height and 1000-kernel weight. The proposed recurrent selection procedure appears effective to improve the population and to extract superior genotypes for varietal development.     

Genotypic variation for competitive ability in spring wheat

Herbicides are the primary method of weed control for crop production in developed countries. For economic and environmental reasons alternative control strategies are being devised. One of these strategies is the development of competitive crop cultivars. The objectives of this research were to establish whether spring wheat (Triticum aestivum L.) genotypes differed in competitive ability and if those differences were related to specific growth characteristics. Sixteen genotypes of spring wheat were grown under simulated weed competition conditions at Saskatoon, Canada over a 3–year period. Four high and four low tillering genotypes from each of two crosses (Neepawa/M1417 and Ingal/M1417) were studied. Weeds consisted of cultivated oat (Avena saliva cv. ‘Waldern’) and oriental mustard (Brassicajuncea cv. ‘Cutlass’) sown at two densities (48 and 96 seeds/m² per weed species). Seedling establishment, ground cover, and seed yield for the three species were determined, as was wheat tiller number, spike number, maximum height, leaf area index, leaf orientation, and flag leaf length and size. Significant (P = 0.001) weed rate by genotype interactions involving changes in genotype rank were detected for wheat grain yield, indicating that the 16 wheat genotypes differed in competitive ability. Wheat grain yield reductions averaged over the two weed densities ranged from 45% to 59%. The highest-yielding genotypes under weed-free conditions were not necessarily the highest yielding under weedy conditions. Genotypes which suffered smaller yield reductions were more effective in suppressing weed growth. Although competitive genotypes were generally taller than non-competitive genotypes, other traits such as large seedling ground cover and flag leaf length were associated with wheat yield under competitive conditions.     

Genetic improvement of spring barley cultivars grown in eastern Canada from 1910 to 1988

Summary Spring barley is an important feed crop in eastern Canada, and the development of high-yielding, high grain protein cultivars is desirable. This study was conducted to assess the impact of breeding on the yield and protein aspects of cultivar development, and to identify related changes in plant characteristics which may have been altered over time. A 3-year field experiment was conducted to evaluate twenty six-rowed spring barley genotypes representing the majority of cultivars developed from 1910 to 1988 for eastern Canada. The yields of barley cultivars released from 1935 to 1988 increased at a rate of about 0.03 t ha^-1 yr^-1, and showed no evidence of having reached a plateau. Increases in yield were associated with higher total dry matter production and harvest index, reduced plant height and increased lodging resistance. No consistent change in main stem or tiller yield components was observed. Grain protein concentration decreased progressively with time, especially with the newer cultivars. Reduction in grain protein concentration was not associated with lower protein content on a per grain basis, but rather with an increase in the amount of non-structural carbohydrate per grain. Total plant and grain N accumulation showed positive trends with time. No trends were observed for N harvest index, apparent post-heading N uptake, N retranslocation, and retranslocation efficiency. Thus, while the newer cultivars accumulated more total and grain N, proportional N partitioning to the grain was not altered.     

Ontogenetic analysis of yield components and yield stability of durum wheat in water-limited environments

Summary One main reason for the slow improvement of durum wheat in water-limited environments is the lack of clear understanding of the interrelationships among yield components and their compensatory changes under low and erratic moisture availability. Five cultivars, varying in many physiological attributes, were tested under different drought-stress conditions in field and greenhouse experiments. The cause-effect relationships of duration of vegetative period, duration of grain-filling period, number of spikes per m², kernels per spike, kernel weight and grain yield per m² were assessed. Furthermore, yield stability was evaluated. Yield reduction was largest under mid-season stress (58%), followed by terminal stress (30%) and early stress (22%). Cultivar Po was very sensitive to terminal stress.Path-coefficient analysis revealed a complex pattern of relationships among the six variables. An increase in vegetative period reduced the grain-filling period under all conditions. It increased number of kernels per spike under non-stress conditions. The direct effect of spikes per m² on grain yield was significantly positive. However, more spikes per m² resulted in fewer kernels per spike and a low kernel weight and, as a result, a negative relationship with grain yield under early stress. Grain-filling period had a strong influence on grain yield via kernel weight. Kernels per spike had the largest direct effect on grain yield. However, it was negatively correlated with kernel weight, especially under terminal stress. Grain yield heavily depended on kernels per spike under early stress and grain-filling period and kernels per spike under terminal stress.Variation in drought susceptibility index among cultivars was significant under early and terminal stress conditions, but not under mid-stress conditions. Yield potential and stability were not correlated for the different drought-stress conditions.Longer grain-filling period, increased number of kernels per spike and limited spike number per m² can be used as selection criteria for sustainable yield in water-limited environments.     

Characterization of malting barley cultivars with more or less stable grain protein content under varying environmental conditions

The grain protein content (GPC) in barley is influenced to a high degree by the environment and this may cause problems if the barley is intended for malt. Barley used for malt should have a GPC not exceeding 11.5% under any environmental condition. It is, therefore, important to breed for cultivars with low and less environmentally influenced GPC. In a 2-year field experiment seven spring barley (Hordeum vulgare L.) cultivars, with varying GPC stability, were grown at two or three nitrogen application rates, respectively, to determine the differences in leaf area development, N-uptake, yield components, GPC and GPC stability. The time course of leaf area index was followed by measuring light transmission and light reflectance. Total shoot N and biomass and yield components were determined by sub-sampling an area of 3×0.25 m⁻² at heading and at maturity. No clear differences were observed in leaf area development between cultivars with low or high GPC stability, but two of the three stable cultivars showed an earlier reduction of the leaf area and less variance in leaf area index during grain filling. Cultivars with low GPC stability tended to be late, had more biomass accumulated at heading, a larger number of ears per m², and a higher yield and a lower GPC than more sensitive cultivars. From the results of a factor analysis an ideotype for low and stable GPC is postulated. This type is characterized by late heading, many tillers and many seeds per ear. It is suggested that breeding for a prolonged vegetative period would reduce the environmental effects on GPC.