Impact of soil fumigation practices on soil nematodes and microbial biomass

This study was designed to understand the impact of methyl bromide (MB) (CHaBr) and its alternatives on both free-living and root-knot nematodes in the soil. A randomized complete block experiment with six treatments and 4 replicates (each replicate in a separate greenhouse) was established in Qingzhou, Shandong Province, China. In addition to MB and untreated control (CK) treatments there were four alternative soil fumigation practices including MB virtually impermeable films (VIF), metam sodium (MS), MS VIF and soil solarization combined with selected biological control agents (SS BCA). Two tomato (Lycopersicum esculentum Mill.) cultivars, cv. Maofen-802 from the Xian Institute of Vegetable Science, China, and cv. AF179 Brillante from the Israeli Hazera Quality Seeds, were selected as test crops. The results indicated that Rhabditidae was the most dominant population with percentage abundance as high as 85% of the total number of identified free-living nematodes, followed by that of Cephalobidae. Methyl bromide and its alternatives except for the non-chemical SS BCA treatment controlled the target pest, root-knot nematodes. Also, the impact of the three chemical alternatives on free-living nematode number and functional group abundance was similar to the impact associated with a typical methyl bromide application. Chemical fumigation practices, especially that with MB, significantly reduced the number of nematodes in the soil and simultaneously significantly reduced the number of nematode genera thereby reducing nematode diversity. All the four soil chemical fumigation activities decreased soil microbial biomass and had an obvious initial impact on microorganism biomass. Furthermore, both plant-parasitic and fungivore nematodes were positively correlated with soil microbial biomass.     

Effect of soil amendments on fractionation of Selenium‐enriched soil

Abstract

This study was to determine the effect of soil amendments on the fractionation of selenium (Se) using incubation experiments under simulated upland and flooded conditions. The treatments were as follows: 1) control [soil + sodium selenite (Na₂SeO₃) (1 mg Se kg^‐1)]; 2) control + calcium carbonate (CaCO₃) (5 g kg^‐1); 3) control + alfalfa (40 g kg^‐1); and 4) control + CaCO₃ (5 g kg^‐1) + alfalfa (40 g kg^‐1). After a 90‐day incubation, soil was sampled and fractionated into five fractions: 1) potassium sulfate (K₂SO₄)‐soluble fraction (available to plants); 2) potassium dihydrogen phosphate (KH₂PO₄)‐exchangeable fraction (potentially available); 3) ammonium hydroxide (NH₃H₂O)‐soluble fraction (potentially available); 4) hydrochloric acid (HCl)‐extractable fraction (unavailable); and 5) residual fraction (unavailable). Compared with the control, CaCO₃ increased the K₂SO₄ fraction at the expense of the NH₃H₂O fraction. Alfalfa increased both the K₂SO₄ and residual fractions but reduced the KH₂PO₄ and NH₃H₂O fractions. The CaCO₃‐alfalfa treatment had a similar effect to the alfalfa treatment alone. The comparison between the upland and flooded conditions showed that the flooded condition generally increased the residual fraction and decreased the potentially‐available fractions. In general, CaCO₃ was a better amendment because it not only increased the available fraction but also maintained the potentially available fractions at a high level. The application of Na₂SeO₃ and use of appropriate soil amendments can improve Se availability in soil.     

Effects of soil compaction and water‐filled pore space on soil microbial activity and N losses

Abstract

Soil compaction is a significant production problem for agriculture because of its negative impact on plant growth, which in many cases has been attributed to changes in soil N transformations. A laboratory experiment was conducted to study the effect of soil compaction and water‐filled pore space on soil microbial activity and N losses. A hydraulic soil compaction device was used to evenly compress a Norfolk loamy sand (fine‐loamy, siliceous, thermic Typic Kandiudults) soil into 50 mm diameter by 127 mm long cores. A factorial arrangement of three bulk density levels (1.4, 1.6, and 1.8 Mg/m³) and four water‐filled pore space levels (60, 65, 70, 75%) was used. Fertilizer application of 168 kg N/ha was made as 1.0 atom % ¹⁵N as NH₄NO₃. Soil cores were incubated at 25°C for 21 d. Microbial activity decreased with both increasing water‐filled pore space and soil bulk density as measured by CO₂‐C entrapment. Nitrogen loss increased with increasing bulk density from 92.8 to 334.4 g N/m³ soil at 60% water‐filled pore space, for 1.4 and 1.8 Mg/m³, respectively. These data indicate that N loss and soil microbial activity depends not only on the pore space occupied by water, but also on structure and size of soil pores which are altered by compaction.     

Studies on soil fumigation—I: Effects on ammonium,nitrate and phosphate in soil and on the growth,nutrition and yield of wheat

Fumigation of field soil with chloropicrin alone or followed by methyl bromide, each at 220Kg·ha^?1, released 20–30 parts/10⁶ NH⁺₄-N which persisted for 75 days; such fumigation also doubled the amount of bicarbonate-extractable phosphate 28 days after fumigation. Soil fumigation increased both the vegetative and grain yields as well as increasing the content of N in the grain and the content of K and Cl in the tops at ear emergence. Root growth and the phosphate uptake activity of the roots were increased by soil fumigation.     

Effects of concentrated application of soil conditioners on soil–air permeability and absorption of nitrogen by young peach trees

ABSTRACT

To study the effects of concentrated application of two soil conditioners, two-year-old peach trees (Prunus persica L.‘Chunmei’) were selected to test the soil air permeability, ¹⁵N absorption and the growth of trees. The experiment comprised three treatments involved concentrated applying either polyacrylamide (treatment I) or Agri-SC (a proprietary soil conditioner, treatment II) at the bottom of each pit or neither of the two (treatment III). And then the whole pit was back-filled with soil. Neither digging a hole nor use of soil conditioners as the control (CK). The results showed that volumetric oxygen content in gases in 5–10 cm soil layer upon concentrated application layer was significantly higher in treatments I and II than that in CK. Soil volumetric water content upon concentrated application layer was higher in treatments I and II than that in CK. Compared with CK, no matter root activity, leaf area, leaf chlorophyll content, or leaf net photosynthesis rate in treatments I and II increased in August and October, which promoted the growth of new shoots and the stem. Compared with CK, the leaf superoxide dismutase activity increased 31.24%, 22.66% and 4.74%, Guaiacol peroxidase increased 21.88%, 13.25% and 3.39%, Catalase increased 11.80%, 7.92% and 1.24% respectively in treatments I, II and III in October. As a result, values of the total roots surface area, total root volume, number of root tips, dry matter accumulation, and organ nitrogen content were markedly higher in treatments I and II than that in CK. And the ¹⁵N utilization rate significantly increased 24.22% and 10.40% respectively in treatments I and II. The result suggested that concentrated application of soil conditioners formed a rhizosphere water storage and breathable layer that not only stores moisture but is also permeable to air. That, in turn, promotes plant growth, increases the nitrogen use efficiency.     

Influence of soil particle size and grinding on bray‐2 extractable phosphorus

Abstract

The influence of soil particle size and soil fine grinding on Bray‐2 extractable phosphorus (Bray‐2P) was studied. Air‐dried and 2‐mm mesh‐sieved soil was separated into six particle size classes: <0.075, 0.075–0.106, 0.106–0.25, 0.25–0.425, 0.425–0.85, and 0.85–2 mm. The lowest amounts of Bray‐2P were found in the 0.425–0.85 and 0.85–2 mm fractions and the highest in <0.075 mm fraction. When ground for 3 min, the amount of Bray‐2P increased in the fractions larger than 0.25 mm, whereas it decreased in the fractions smaller than 0.25 mm. In the large fraction (0.425–0.85 mm), grinding for 1 to 3 min led to an increase in the amount of Bray‐2P, but grinding for 9 to 18 min caused a decrease. In contrast, in the small fraction (<0.075 mm), the amount of Bray‐2P decreased by grinding for 1 min. The large and small fractions that were ground absorbed P in proportion to the grinding time during the extraction‐filtration period.     

Effect of α-casein on DNA adsorption by Andosols and by soil components

Andosols and the soil components (allophanes, humic acids, and goethite) had been autoclaved to destroy the nuclease activity of soil microflora. DNA adsorption by allophanes and Andosols was decreased by increasing the amount of α-casein added to the allophanes and to soils up to casein concentration of 5 mg ml^?1. DNA adsorption by humic acids was significantly increased by increasing the amount of α-casein up to 1.0 mg ml^?1, whereas the addition of 20 mg α-casein ml^?1 completely blocked DNA adsorption. These results can explain why the addition of excess skim milk is operationally needed for effective DNA extraction from Andosols. The amount of DNA adsorbed by Andosols treated with dephosphorylated α-casein was significantly higher than that of not treated Andosols (p?相似文献   

Effects of freeze–thaw cycles and the soil water content on carbon and nitrogen changes in different soil types of Heilongjiang Province,China

To reveal the influence of freeze–thaw cycles (FTCs) on soil carbon and nitrogen changes, six typical soils in Northeast China were selected as the research objects to conduct a FTC simulation test in an artificial climate chamber. Three soil volumetric water contents (10%, 20%, 30%) and eight FTCs (0, 2, 4, 6, 8, 10, 15, 20) were set. The results showed that the soil organic carbon (SOC) and microbial biomass carbon (MBC) contents of different soil types under the FTCs initially exhibited a downward and then an upward trend, while the dissolved organic carbon (DOC) content exhibited an upward and then a downward trend. Otherwise, the fourth and sixth FTCs were the key points of change. The SOC, MBC and DOC contents in paddy fields were higher than those in dry fields, showing upward and then downward trends spatially from northeast to southwest. The SOC and MBC contents in each soil type were the highest at the 20% water content, and the DOC content gradually increased with increasing water content. The ammonium nitrogen (NH₄⁺-N) content in different soil types at different water contents under the FTCs showed an upward trend first, then a downward trend and finally an upward trend. The NH₄⁺-N content in paddy fields was higher than that in dry fields. The nitrate nitrogen (NO₃^‒-N) content showed a downward trend first, then an upward trend and finally a downward trend. The NO₃^‒-N content in dry fields was higher than that in paddy fields. The NH₄⁺-N contents in the three soil types on the Sanjiang Plain were significantly higher than those on the Songnen Plain. The NH₄⁺-N and NO₃^‒-N contents showed upward trends with increasing water content, but the differences were not significant. The results have implications for the study of different types of soils and provide references for research on the mechanism of soil carbon and nitrogen transformation in typical farming areas in Northeast China.     

Addition of organic and inorganic P sources to soil – Effects on P pools and microorganisms

Phosphorus deficiency is wide-spread due to the poor solubility of soil P and the rapid formation of poorly available P after P addition. Microbes play a key role in soil P dynamics by P uptake, solubilisation and mineralisation. Therefore a better understanding of the relationship between type of P amendment, microbial activity and changes in soil P pools is important for a better management of soil P. A P deficient soil was amended with two composts (low P or high P), two crop residues (low P or high P), and inorganic P (KH₂PO₄) at low and high P, and incubated for 56 days. Composts were added at 20 g kg⁻¹ resulting in a total P addition of 4.1 mg kg⁻¹ soil with the low P compost and 33.2 mg kg⁻¹ soil with the high P compost. The same amount of P was added with the other amendments (residues and inorganic P). All amendments increased cumulative respiration, but microbial biomass and the abundance of bacteria and fungi (assessed by phospholipid fatty acid analysis) increased significantly only in soils with organic amendments, with greater increases with residues. The concentration of the inorganic P pools NaHCO₃-P_i, NaOH-P_i and HCl-P increased significantly within 5 h after amendment, particularly with high P amendments. Over the following 56 days, labile inorganic P was converted mainly into non-labile inorganic P with inorganic P addition whereas labile and non-labile organic P was formed with organic amendments. It is concluded that organic P sources, particularly those with high P concentration can stimulate the formation of organic P forms in soils which may provide a long-term slow release P source for plants and soil organisms.     

Studies on microörganisms and their activities in soil Part. 2. Virgin and cultivated soil of the same origin

The nature of soil is modified differently depending upon the artificial condition such as its utilization or management. It is therefore expected that the microbiological characteristic of soil is changed also. Greaves¹⁾ and Williams²⁾ reported that the reclamation of virgin soil brought about a change of bacterial count. Suzuki et al³⁾ observed that the kind of fungi differed between a virgin and a cultivated soil and that the vegetative mycelium was numerous in the former than in the latter. On the other hand, according to W aksman and Starkey⁴⁾, the bacterial count differed depending upon the fertility of soil. Singh⁵⁾ reported also that the number of fungi and actinomycetes was higher in a fertile than in an infertile soil. Lochhead⁶⁾, and Lochhead and Chasen studied the bacterial flora of a fertile (long-continued application of manure) and an infertile (no fertilizer for many years) soil and found that a certain difference could be observed when morphological, physiological and nutritional classification are tried.     

Effect of vegetation changes on soil erosion on the loess plateau

Vegetation is one of the key factors affecting soil erosion on the Loess Plateau. The effects of vegetation destruction and vegetation restoration on soil erosion were quantified using data from long-term field runoff plots established on the eastern slope of the Ziwuling secondary forest region, China and a field survey. The results showed that before the secondary vegetation restoration period （before about 1866-1872）, soil erosion in the Ziwuling region of the Loess Plateau was similar to the current erosion conditions in neighboring regions, where the soil erosion rate now is 8 000 to 10 000 t km^-2 year^-1. After the secondary vegetation restoration, soil erosion was very low; influences of rainfall and slope gradient on soil erosion were small; the vegetation effect on soil erosion was predominant; shallow gully and gully erosion ceased; and sediment deposition occurred in shallow gully and gully channels. In modern times when human activities destroyed secondary forests, soil erosion increased markedly, and erosion rates in the deforested lands reached 10 000 to 24000 t km^-2 year^-1, which was 797 to 1682 times greater than those in the forested land prior to deforestation. Rainfall intensity and landform greatly affected the soil erosion process after deforestation. These results showed that accelerated erosion caused by vegetation destruction played a key role in soil degradation and eco-environmental deterioration in deforested regions.     

Impact of organic and inorganic nutrition on soil–plant nutrient balance in arecanut (Areca catechu L.) on a laterite soil

The study assessed the impact of continuous application of vermicompost and chemical fertilizers nitrogen, phosphorus and potassium (NPK) on arecanut in India. Key parameters examined were biomass production, nutrient uptake, yield, soil fertility and net benefit. Pooled analysis of 8-year data revealed that nutrient application registered significantly higher yield (2585–3331 kg ha^?1) than no nutrition (1827 kg ha^?1). Yields in organic nutrition were around 85% of the yields obtained in inorganic NPK. The concentrations of leaf N and K were significantly higher with NPK than with vermicompost. Vermicompost significantly increased soil organic carbon and the availability of calcium (Ca), magnesium (Mg), manganese (Mn) and copper (Cu), but reduced exchangeable K in soil. The total uptake of K and Ca together contributed positively to 75% variability in total biomass production. Nutrient removal of iron (Fe), P, K and Cu positively influenced the yield with about 81% variability. Biomass partitioning and nutrient uptake pattern are important for fertilization program of arecanut.     

Influence of two “nitrogen regulators”; on soil n transformations and movement

Abstract

Experiments were conducted to assess the potential influence of a commercial product, EXTEND, on nitrogen transformations and movement in a sandy soil. Neither nitrapyrin (a commercially‐available nitrification inhibitor) nor EXTEND significantly affected the rate of NH₄ ⁺‐N or NO₃ ^‐‐N movement through a column of soil treated with urea‐ammonium nitrate liquid fertilizer. Nitrapyrin effectively inhibited nitrification, but the nitrification rate in the EXTEND treated systems were the same as control.     

Using micro focus industrial computed tomography to characterize the effects of soil type and soil depth on soil pore characteristics,morphology, and soil compression in Xi’an,China

Journal of Soils and Sediments - Land subsidence has caused serious geological damage in many countries, including China. Soil pore number, size, shape, and pore size distribution affect soil...     

Comparison of phosphate rock and triple superphosphate on a phosphorus‐deficient Kenyan soil

Abstract

Soil phosphorus (P) deficiency is a constraint to crop production in many regions of sub‐Saharan Africa, which could be overcome through use of either soluble P fertilizer or sufficiently reactive phosphate rock (PR). A field study was conducted with corn (Zea mays L.) for three growing seasons (18 months) on a P‐deficient, acid soil in Kenya to compare a soluble P source (triple superphosphate, TSP) and relatively reactive Minjingu PR from Tanzania. In the 18 months following application of 250 kg P ha^‐1, bicarbonate extractable inorganic soil P (P_i) was higher for application of TSP than PR, but Pi extracted with a mixed anion‐cation resin was comparable for TSP and PR. Inorganic P extracted by 0.1M NaOH, without prior extraction of resin and bicarbonate P_i, decreased during the 18 months following TSP application, but increased following PR application. After 18 months, about 7% of the added PR‐P remained as Ca‐bound P that was extracted with 1M HCl. The 1M HCl extractable P., however, underestimated residual PR‐P that gradually dissolved and supplied plant‐available P, as indicated by recovery of <40% of PR‐P added to soil in laboratory incubations even though PR solubility in HCl was >90%. Minjingu PR was an effective source of P for corn. Corn yields were comparable for TSP and PR, and the relative agronomic effectiveness of PR averaged 107% in Season 1 and 79% in Season 3. Anion resin and mixed anion‐cation resin appeared to be superior to bicarbonate and NaOH as a soil P test for use with both TSP‐ and PR‐treated soils.     

The influence of brine contamination and phospho‐gypsum amendments on soil chemical properties and plant response

Abstract

Phosphogypsum (PG), the by‐product of wet acid production of phosphoric acid from rock phosphate, was found to be effective as a soil conditioner for sodic, solonets, and solonetzic soils. Unlike mine gypsum, PG contains impurities whose release into soil and ground water need to be monitored. PG was incorporated with soils contaminated with oilfield brine to determinate the influence of PG amendments and brine contamination on soil chemical properties and plant response in a growth chamber. Application rates of PG were 0, 25, 50, 100, 200, and 400 Mg ha^‐1 and the testing crop was Barley (Hardeum vulgare L.). Phosphogypsum provides a means of remediating brine spills and can result in benefits expressed as increased plant growth. Soil salinity of brine contaminated soils was ameliorated as evidenced by reduced the electrical conductivity, SAR and exchangeable Na levels in 0–15 cm depth of the columns. There is no evidence that application of PG increased the level of trace elements in plants. Contamination of soil with brine spill is the major factor dominating plant growth and soil‐plant element content. From plant growth point of view, two elements could be excessive, namely Boron (B), and Cadmium (Cd). The high level of B in the plant arose from brine spill and the cause for the excessive Cd level in barley grain is unknown and currently under investigation. The distribution of trace elements in soil profiles demonstrated that there was little, if any, potential for movement of trace elements into greater depths.     

The influence of soil aeration on growth and elemental absorption of greenhouse‐grown seedling pecan trees

Abstract

‘Dodd’ pecan seedlings were exposed to 3 levels of soil aeration for 30 days; 100%, 5%, and 0% of the container surface exposed to the atmosphere. These treatments resulted in about 21%, 13.5%, and 3% soil O₂and 0.3%, 5%, and 13% soil CO₂for 100%, 5%, and 0% of the container surface exposed, respectively. Restricting soil aeration induced partial stomatal closure, and decreased leaf number, leaf area, and leaf, trunk and root dry weights. The decrease in root dry weight associated with reduced soil aeration exceeded the decrease in top dry weight by about 50%. Translocation of N and P to the leaves was reduced when soil aeration was restricted, but root N and P concentrations were increased compared to trees grown in well aerated soil. Leaf elemental concentrations of Ca, Mg, and Mn were lower when trees were exposed to reduced soil aeration. Zinc and Fe concentrations were greater in the roots of trees with low aeration, but leaf and trunk concentrations of Zn and Fe were not affected     

Studies on the ecology of actinomycetes in soil—VIII: Distribution and characteristics of acidophilic actinomycetes

Acidophilic actinomycetes, growing between pH 3.5 and 6.5, were isolated from 17 natural soils and mine wastes. Most isolates were Streptomyces spp. and selected ones were compared with some established neutrophilic species by assessment of overall similarity. Acidophilic and neutrophilic strains clustered separately, differing in a number of cultural and physiological characters, in addition to pH requirements. The possible importance of acidophilic actinomycetes in soil is discussed.     

Influence of poultry manure–derived biochars on nutrients bioavailability and chemical properties of a calcareous soil

This study was conducted to investigate the effects of poultry manure (PM) and its derived biochars on chemical properties of a calcareous soil. PM and biochars prepared at 200°C (B200), 300°C (B300) and 400°C (B400) were applied to a calcareous soil at 2% level (w/w) and incubated for 150 days. Selected soil chemical properties and phosphorous, potassium, iron, manganese, zinc and copper availability and recovery were determined at 1, 15, 45 and 150 days of incubation. Soil nutrients availability, organic carbon (OC), electrical conductivity (EC) and cation exchange capacity (CEC) increased by addition of all organic substances. Biochars prepared at higher temperatures were more effective in increasing soil OC with higher durability compared to other treatments. The addition of PM and B200 decreased soil pH, whereas B400 increased it. Although the highest soil EC was observed in B300- and B400-treated samples in the early stages of incubation, the rate of increase in soil EC was higher for PM- and B200-treated soils compared to other treatments. It was concluded that biochar prepared at 300°C had the highest positive effect on nutrients availability and lasts longer in calcareous soil compared to the other produced biochars and PM.