Effects of incorporating cowpea in a subtropical grass pasture on forage production and quality and the performance of cows and calves

The increasing cost of N fertilizer has stimulated an interest in sourcing protein from warm‐season legumes among beef cattle producers in the tropical/subtropical areas of the world. The objective of this study was to evaluate effects of two strategies of incorporating cowpea [Vigna unguiculata (L.) Walp.] into bahiagrass (Paspalum notatum Flügge) pastures on the herbage characteristics and performance of grazing cow–calf pairs. The study was conducted in Ona, Florida, USA, from May to August in 2007 and 2008. Experimental units were 1·0 ha. Treatments were bahiagrass pasture alone (control), 50:50 bahiagrass–cowpea pasture (cowpea), bahiagrass pasture with a cowpea creep grazing area (0·1 ha, creep grazing) and bahiagrass pasture with a creep‐fed concentrate [(creep feeding; 10 g kg^?1 body weight (BW)]. The cowpea pastures had lower herbage mass [HM, 1·8 vs. 3·7 t ha^?1] and herbage allowance [HA, 0·8 vs. 1·4 kg DM kg^?1 live weight (LW)] compared with the other treatments. Cowpea had greater CP (CP, 160 g kg^?1) and in vitro digestible organic matter (IVDOM), (600 g kg^?1) than bahiagrass (110 and 490 g kg^?1 respectively); however, cowpea HM was only 0·9 t ha^?1 in May and 0·7 t ha^?1 in June, but it did not persist in July and August. Calves receiving the creep feeding treatments had greater average daily gain (0·8 vs. 0·7 kg d^?1) than calves in other treatments. Further research is necessary to exploit the superior nutritive value of cowpea in grazing systems in the south‐eastern USA.     

Effects of feeding different ensiled forages on the productivity and nutrient-use efficiency of finishing lambs

The effects of offering ensiled red clover (Trifolium pratense), lucerne (Medicago sativa), pea (Pisum sativum), kale (Brassica oleracea) and hybrid ryegrass (Lolium hybridicum) on the productivity and nutrient‐use efficiency of lambs were investigated. Forages were cut, wilted for 24 h and ensiled as round bales. A hundred Suffolk‐cross lambs, aged 8 months, were offered grass silage during a 5‐week standardization period and then group‐housed for 14 d and offered ad libitum access to a treatment silage. For the measurement period, lambs were split into four replicate groups of five lambs per treatment. Dry matter intake and live weight were recorded every 7 d over 56 d. The chemical composition of the silages differed for all the variables measured. Lambs offered pea silage had a very low liveweight gain and this treatment was discontinued after 21 d. Lambs offered the other forages had a higher liveweight gain than lambs offered ryegrass silage (P < 0·001). Food conversion and nitrogen‐use efficiency were higher in lambs offered the red clover, lucerne and kale silages compared with those offered ensiled ryegrass (P < 0·001). These findings demonstrate the potential for using ensiled alternative forages rather than ryegrass to increase the productivity and nutrient use efficiency of livestock systems.     

Ingestion and excretion of nitrogen and phosphorus by beef cattle under contrasting grazing intensities

There is increased global interest in the environmental impacts of farming, including the need to prevent the contamination of soil, water and air with excessive amounts of nitrogen (N) and phosphorus (P) in grazed systems. Reduction in grazing intensity has frequently been recommended to meet biodiversity and production goals in sustainable grazing systems. The objective of this experiment was to measure N and P ingestion and excretion by beef cattle grazing semi‐natural pastures at two grazing intensities (Moderate or Lenient). The cattle grazing at Moderate grazing intensity had significantly more defaecations each day than those grazing at Lenient intensity (9·5 vs. 7·5) and tended to have more urinations (7·0 vs. 5·8). For the Moderate and Lenient treatments, respectively, 113 vs. 76 g N d^?1 was excreted compared with 136 vs. 94 g N d^?1 ingested; 12 vs. 8 g P d^?1 was excreted compared with 13 vs. 10 g P d^?1 ingested and urine N comprised 0·51 and 0·52 of the total N excreted each day. In improved, intensively managed grassland systems, urine N comprises a much higher proportion (approximately 0·70–0·85) of the daily total N excreted. The lower level found here is likely to impact on potential volatilization, denitrification and leaching losses, and these aspects should be examined further to see the extent to which semi‐improved grasslands containing increased plant diversity compared with improved grasslands can deliver higher resource protection, as well as enhanced grassland faunal diversity and abundance.     

不同作物茬口对冬油菜养分积累和产量的影响

为探究关中平原地区不同作物茬口对冬油菜养分积累和产量的影响,通过两年田间试验,研究分析不同茬口,包括休闲茬口(FW)、大豆茬口(SW)、毛苕子茬口(HW)和玉米茬口(MW)对冬油菜土壤养分含量、地上部干物质积累、分配和氮磷养分吸收积累、产量构成因素以及产量的影响,筛选冬油菜生产适宜的前茬作物,为加强冬油菜生产,提高土地...     

Yield response of long-term mixed grassland swards and nutrient cycling under different nutrient sources and management regimes

Abstract The response of a long‐term, mixed‐species hayfield in Maine, USA, to commercial fertilizers and liquid dairy manure was evaluated over a 6‐year period, including the effects on yield, nutrient concentration and cycling, forage species composition and soil nutrient levels. Nutrient treatments included an unamended control, N fertilizer, NPK fertilizer and liquid dairy manure (LDM). The application rates of plant‐available N, P, and K were constant across treatments. Application of nutrients in any form increased forage yield, generally by 2–4 t dry matter (DM) ha^?1 year^?1. Yield from NPK fertilizer was 0·05–0·25 higher than from LDM, due to differences in N availability. Yield responses to P and K were minimal and there appeared to be no difference between P and K in fertilizer and manure. The forage sward became increasingly dominated by grass species as the experiment progressed; application of P and K in fertilizer or LDM allowed Agropyron repens to increase at the expense of Poa pratensis. Forage nutrient removal accounted for all applied N and K, and nearly all applied P, throughout the study period, demonstrating the important role these forages can play in whole‐farm nutrient management.     

Effects of predicted milk yields sustained by grazed grass on dairy cow performance and concentrate requirements throughout the grazing season

The objective of this study was to examine the effects of predicted milk yields sustained by grazed grass (‘Milk‐from‐Grass’: LOW, MED or HIGH) and Parity (primiparous or multiparous) on the performance and concentrate requirements of grazed Holstein‐Friesian dairy cows offered concentrates on a ‘feed‐to‐yield’ basis during the grazing season. The mean Milk‐from‐Grass values assigned to cows in the LOW, MED and HIGH treatment groups (sixteen multiparous and eight primiparous cows per group) during the experiment (24 May to 2 October; 131 d) were 12·7, 15·4 and 18·1 kg cow^?1 d^?1, respectively. Concentrate allocations were adjusted every two weeks (approximately) based on individual cows’ milk yields, and concentrate was offered at a rate of 0·45 kg for each kg of milk produced above the assigned Milk‐from‐Grass value at that time. Increasing Milk‐from‐Grass from LOW to MED to HIGH decreased both the mean daily concentrate allocations (4·90, 3·17 and 1·79 kg cow^?1 d^?1, respectively) and milk yields (23·8, 21·8 and 19·0 kg cow^?1 d^?1) of the cows. Cows in the LOW treatment group had lower mean and final (i.e. at the end of the study) BCS and LW than those in the MED or HIGH groups. The effects of Milk‐from‐Grass were similar for both primiparous and multiparous cows.     

Competition between Artemisia frigida and Cleistogenes squarrosa under different clipping intensities in replacement series mixtures at different nitrogen levels

The relative competitive ability of Artemisia frigida Willd. (C₃, semi‐shrub) and Cleistogenes squarrosa (Trin.) Keng (C₄, perennial bunchgrass), grown in a greenhouse, was studied under three nitrogen fertilizer levels, three defoliation intensities and five competitive replacement series in a factorial arrangement. Relative yield total and the respective crowding coefficients for each species were calculated with reference to aboveground, below‐ground and total biomass. The index of aggressivity was also determined relative to total biomass. Cleistogenes squarrosa was a consistently weak competitor at all nitrogen fertilizer and clipping levels compared with A. frigida. Artemesia frigida had a higher relative yield when grown in mixtures with C. squarrosa than in monocultures, whereas the yield of C. squarrosa was lower in mixtures with A. frigida than in monocultures. The relative competitive ability of A. frigida over C. squarrosa declined significantly at different clipping intensities with increasing nitrogen fertilizer. The interaction of nitrogen fertilizer with clipping was significant, although the clipping effect on aggressivity was not significant.     

Canopy height and N affect herbage accumulation and the relative contribution of leaf categories to photosynthesis of grazed brachiariagrass pastures

The effects of three canopy heights (10, 25 and 40 cm) and two N rates (50 and 250 kg ha^?1 year^?1) on herbage accumulation (HA), participation of leaf categories in the leaf area index (LAI) and photosynthesis of grazed “Mulato II” brachiariagrass (Brachiaria brizantha × B. decumbens × B. ruziziensis) were investigated during two summer growing seasons in Piracicaba, São Paulo, Brazil. The HA and LAI increased linearly from 8560 to 13600 kg DM ha^?1 year^?1 and 2.3 to 5.5 m² m^?2, respectively, as canopy height increased. Mulato II brachiariagrass maintained at 10 cm canopy height showed greater proportion of young leaves, but was less productive than taller canopies. Taller canopies had greater proportion of mature leaves in the LAI as well as greater photosynthesis. The greater N rate contributed to increased LAI, leaf and canopy photosynthesis rates and HA but did not affect the LAI composition. The total leaf area is more important to HA than the proportion of young leaves in the LAI, as taller canopies were more productive, suggesting that maintaining Mulato II brachiariagrass at 25 or 40 cm is advantageous for this grass.     

Herbage and nitrogen yields,fixation and transfer by white clover to companion grasses in grazed swards under different rates of nitrogen fertilization

In grass–legume swards, biologically fixed nitrogen (N) from the legume can support the N requirements of the grass, but legume N fixation is suppressed by additional fertilizer N application. This study sought to identify a fertilizer N application rate that maximizes herbage and N yields, N fixation and apparent N transfer from white clover to companion grasses under intensive grazing at a site with high soil‐N status. During a 3‐year period (2011–2013), swards of perennial ryegrass and of perennial ryegrass–white clover, receiving up to 240 kg N ha^?1 year^?1, were compared using isotope dilution and N‐difference methods. The presence of white clover increased herbage and N yields by 12–44% and 26–72%, respectively. Applications of N fertilizer reduced sward white clover content, but the effect was less at below 120 kg N ha^?1. The proportion of N derived from the atmospheric N fixation was 25–70%. Nitrogen fixation ranged from 25 to 142 kg N ha^?1 measured using the isotope dilution method in 2012 and from 52 to 291 kg N ha^?1 using the N‐difference method across all years. Fertilizer N application reduced the percentage and yield of fixed N. Transfer of N from white clover to grass was not confirmed, but there was an increased N content in grass and soil‐N levels. Under intensive grazing, the maximum applied N rate that optimized herbage and N yields with minimal effect on white clover content and fixation rates was 60–120 kg N ha^?1.     

The use of herbage nitrogen status to optimize herbage composition and intake and to minimize nitrogen excretion: an assessment of grazing management flexibility for dairy cows

A framework for managing rotationally grazed pastures for dairy cattle which enables the cows’ energy and protein requirements to be met while simultaneously limiting the amount of N excreted in order to reduce N losses is described. The first objective is achieved by ensuring that lamina mass and the N concentration of herbage do not limit herbage intake or feeding value. The second objective is achieved by limiting N fertilizer supply or increasing the interval between defoliations to reduce the N concentration of herbage. Lower and upper thresholds for the N concentration of herbage and lamina mass were estimated from published data. The method is illustrated using two vegetative regrowths (beginning and end of spring) in a cutting experiment with two fertilizer treatments, 0 or 120 kg N ha^?1 (?N and +N), and early or late cutting. Decreasing N supply led to a reduction in grazing management flexibility, i.e. the defoliation interval ranges which were compatible with the required sward characteristics (minimum lamina mass and N concentration of lamina) for herbage intake and to meet the protein requirements of dairy cows. Aiming for the upper threshold N concentration of herbage increased the minimum interval between defoliations only for the +N treatments. Nevertheless, grazing management flexibility remained the highest for the +N treatments.     

Impact of deferred grazing and fertilizer on plant population density,ground cover and soil moisture of native pastures in steep hill country of southern Australia

The impact of deferred grazing (no defoliation of pastures for a period generally from spring to autumn) and fertilizer application on plant population density, ground cover and soil moisture in a hill pasture (annual grass dominated, with Australian native grasses being the major perennial species) were studied in a large‐scale field experiment from 2002 to 2006 in southern Australia. Three deferred grazing strategies were used: short‐term deferred grazing (no defoliation between October and January each year), long‐term deferred grazing (no defoliation from October to the autumn break, that is the first significant rainfall event of the winter growing season) and optimized deferred grazing (withholding time from grazing depends on morphological development of the plants). These treatments were applied with two fertilizer levels (nil fertilizer and 50 kg P ha^?1 plus lime) and two additional treatments [continuous grazing (control) and no grazing for year 1]. Deferred grazing increased (P < 0·05) perennial grass tiller density compared with the control. On average, the tiller density of the three deferred grazing treatments was 27–88% higher than the control. There was a negative (P < 0·01) relationship between perennial and annual grass tiller density. Fertilizer application increased (P < 0·05) legume plant density. The densities of annual grasses, legumes, onion grass (Romulea rosea) and broadleaf weeds varied between years, but perennial grass density and moss cover did not. The ground cover of the deferred grazing treatments in autumn was on average 27% higher than the control. Soil moisture differed between treatments at 15–30 cm depth, but not at 0–15 depth over autumn and winter. The results imply that deferred grazing can be an effective tool for rejuvenating degraded native pastures through increases in native grass tiller density and population and through improving farm productivity and sustainability.     

不同施氮量对春茬厚皮甜瓜生育后期氮磷钾吸收及利用的影响

采用盆栽砂培实验,以“翠甜”厚皮甜瓜为材料,设 6 个氮处理（0、5、10、15、20、25 mmol/L）研究其对海南地区春茬厚皮甜瓜生育后期氮磷钾养分吸收及利用的影响。结果表明：施氮能够显著增加膨大期根、果实氮含量,显著增加生育后期茎叶、果实及全株中的氮、磷和钾积累量,显著增加氮磷钾养分收获指数和单果鲜重,增加磷生理利用效率,显著降低膨大期果实钾含量及氮生理利用效率。钾生理利用效率随氮水平增加先增加后降低,在 10 mmol/L 时达到峰值。本研究中,10 mmol/L 氮处理单果鲜重最大,氮磷钾养分生理利用效率也最高,且氮磷钾养分收获指数居中,最有利于节约施肥,经济效益最大化。     

不同控释氮肥运筹对粳稻养分吸收与氮素利用的影响

以绥粳18为供试材料,通过盆栽试验,在一次性施入控释氮肥免追肥条件下,设置不同施肥量(高、中、低、无)和施肥深度(5 cm、10 cm),探讨不同氮肥施用方式对粳稻养分吸收和氮素利用的影响。结果表明,在不同氮肥运筹条件下,粳稻各生育期氮、磷、钾的吸收、转运均存在明显的协同效应,施肥深度5 cm组,随着氮肥施用量的增加,粳稻的氮吸收总量增加,同时可促进磷的吸收,但影响幅度较小。钾变化与氮、磷变化有所不同。施肥深度10cm组,施高量氮肥抑制了粳稻对磷的吸收、减少了对氮的吸收,但有利于提高氮素利用率,对钾的吸收无显著影响。施用树脂包膜控释肥,纯氮用量为153 kg/hm^2、施肥深度5 cm、一次性施肥免追肥可获得最优水稻氮素利用效率并显著提高磷钾肥利用效率。     

Effect of cover crops and mulches on weed control and nitrogen fertilization in tomato (Lycopersicon esculentum Mill.)

Cover crops and mulches are a suitable choice for sustainable agriculture because they improve weed control and crop performance. The aim of this research was to investigate weed control and nitrogen supply by using different winter cover crop species which were converted into mulches in spring. We carried out a 2-year field experiment where a tomato crop was transplanted into four different types of mulches coming from winter cover crops [hairy vetch (Vicia villosa Roth.), subclover (Trifolium subterraneum L.), oat (Avena sativa L.), and a mixture of hairy vetch/oat)] and in conventional treatment (tilled soil without mulch). The mixture of hairy vetch/oat cover crop produced the highest aboveground biomass (7.9 t ha⁻¹ of DM), while the hairy vetch accumulated the highest N in the aboveground biomass (258 kg N ha⁻¹). The oat cover crop was the most effective cover crop for suppressing weeds (on average −93% of weed aboveground biomass compared to other cover crops). After mowing the cover crop aboveground biomass was placed in strips as dead mulch into which the tomato was transplanted in paired rows. Weed density and total weed aboveground biomass were assessed at 15 and 30 days after tomato transplanting to evaluate the effect of mulches on weed control. All mulches suppressed weeds in density and aboveground biomass compared to the conventional system (on average −80% and −35%, respectively). The oat was the best mulch for weed control but also had a negative effect on the marketable tomato yield (−15% compared to the conventional treatment). Amaranthus retroflexus L. and Chenopodium album L. were typical weeds associated with the conventional treatment while a more heterogeneous weed composition was found in mulched tomato. Legume mulches, in particular hairy vetch, gave the best marketable tomato yield 28% higher than the conventional system both with and without nitrogen fertilization. This research shows that winter cover crops converted into dead mulch in spring could be used successfully in integrated weed management programs to reduce weed infestation in tomato crops.     

实地氮肥管理下的水稻生长发育和养分吸收特性

 以代表性水稻品种为材料，研究了实地氮肥管理模式下水稻的生长发育和养分吸收规律。与农民习惯施肥方法相比，实地氮肥管理在产量不降低甚至有所提高的前提下可以较大幅度地降低施氮量。实地氮肥管理水稻有效穗数有所下降，但其分蘖成穗率明显提高。穗分化前实地氮肥管理水稻叶面积指数和根质量低于农民习惯施肥法，抽穗后结果则相反。实地氮肥管理提高了抽穗期水稻的有效叶面积和高效叶面积的比率、抽穗至成熟的干物质积累、抽穗后根冠比和剑叶的光合速率以及齐穗期群体透光率。实地氮肥管理水稻对氮、磷、钾的吸收高峰均出现在穗分化至抽穗期，此阶段氮、磷、钾的吸收量约占最终总吸收量的45.6%~46.2%、39.6%~43.6％和44.2%～45.2％，均明显高于农民习惯施肥方法。对实地氮肥管理产量提高原因及养分吸收规律进行了讨论。     

Production, survival and nutritive value of the perennial legumes Dorycnium hirsutum and D. rectum subjected to different cutting heights

Dorycnium hirsutum and D. rectum are perennial legumes which may have potential for use as pastures for the control of groundwater recharge in southern Australia. Little is known about the quality of the forage of Dorycnium species for grazing livestock or how these species respond to cutting. The effect of cutting height on plant survival, production of dry matter (DM), the proportion of leaf, edible stem (approximately <5 mm diameter) and woody stem in the DM and the nutritive value of the edible components was investigated. Biomass above five cutting‐height treatments (uncut, ground level, 5–8 cm, 10–15 cm and 15–30 cm above ground level) was removed at 8‐week intervals from plots of D. hirsutum and D. rectum from September 2002 to July 2003. In both species, plants subjected to lower cutting height treatments produced less DM above the height of the cut than those cut at higher heights. DM production declined over time in all treatments. Plants cut to ground level failed to regrow after the second harvest in D. hirsutum and the fourth harvest in D. rectum. Thus, these Dorycnium species were susceptible to high severity defoliations at 8‐week intervals. Negligible inedible woody stem was present in regrowth of both species after 8 weeks but D. hirsutum regrowth had a higher proportion of leaf (0·72) than D. rectum (0·56). Plants left uncut accumulated a large proportion of inedible woody stem in the DM (0·69 in both species) by July 2003, particularly at the base of the plant. Edible DM from regrowth of D. hirsutum and D. rectum had crude protein (CP) concentrations of 120 and 150 g kg^?1 DM; dry matter digestibility (DMD) values of 0·45 and 0·58; organic matter digestibility (OMD) values of 0·50 and 0·64; neutral‐detergent fibre (NDF) concentrations of 370 and 290 g kg^?1 DM; and acid‐detergent fibre (ADF) concentrations of 260 and 210 g kg^?1 DM, respectively. Medicago sativa, grown under similar conditions, had higher digestibility values (0·63 DMD and 0·66 OMD) and similar CP concentrations to D. rectum (140 g kg^?1 DM), but higher concentrations of NDF and ADF (410 and 290 g kg^?1 DM). Leaf material from both Dorycnium species had a higher nutritive value than edible stems, with DMD and OMD values of leaf of D. rectum being 0·68 and 0·74 respectively. Uncut plants had a much lower nutritive value of edible DM than the regrowth from cut treatments; older material was also of a lower nutritive value. The relatively low nutritive value of even the young regrowth of Dorycnium species suggests that forage quality is a major limitation to its use. Forage of Dorycnium species could be used during periods when other sources of forage are in short supply but infrequent grazing it is likely to produce forage of a low nutritive value.     

Effects of cutting or grazing grass swards on herbage yield, nitrogen uptake and residual soil nitrate at different levels of N fertilization

On a Flemish sandy loam soil, cut and grazed swards were compared at different levels of mineral nitrogen (N) fertilization. Economically optimal N fertilization rates were 400 (or more) and 200 kg N ha^?1 yr^?1 on cut and grazed swards respectively. Considering the amounts of residual soil nitrate‐N in autumn, these N rates also met the current Flemish legal provisions, i.e. no more than 90 kg ha^?1 nitrate‐N present in the 0–90 cm soil layer, measured between 1 October and 15 November. The N use efficiency was considerably higher in cut grassland systems than in grazed systems, even when the animal component of a cut and conservation system was included. The results indicate that, for cut grasslands, two N application rates should be considered: intensively managed grasslands with high amounts of N (400 kg ha^?1 yr^?1 or more) or extensively managed grasslands with white clover and no more than 100 kg N ha^?1 yr^?1.     

Influence of different forage grasses on nitrate capture and leaching loss from a pumice soil

Nitrogen (N) uptake and loss in leachate from a pulse of ¹⁵N-labelled synthetic cow urine applied to a pumice soil were compared in a glasshouse lysimeter experiment among the grass species, Agrostis capillaris , Dactylis glomerata , Phalaris aquatica , Lolium multiflorum , L . perenne and a L . multiflorum/A. capillaris mixture. In addition, four L. perenne treatments investigated the effect of infection by Neotyphodium lolii strains. Leachate volumes and leachate nitrate concentrations were measured. At final harvest 24 weeks after sowing, dry matter (DM) mass of shoots and roots, plant N and ¹⁵N contents, soil residual mineral N, and root diameters and lengths were measured. Endophyte had no effect on plant or nitrate variables. Lysimeters planted with L . perenne (pooled endophyte treatments) leached 48 mg of NO₃-N compared with <3 mg N for the other grasses. Recovery of ¹⁵N was highest in A. capillaris (0·99), followed by D. glomerata and P. aquatica (0·89), the L . multiflorum / A. capillaris mixture (0·87), L . multiflorum (0·60) and L . perenne (0·44). Low ¹⁵N recoveries and high leaching losses from L . perenne were associated with low plant and root masses of DM and low rooting depth. High aerial mass of DM, root systems extending below 20 cm and high root masses of DM in lysimeters with A. capillaris , P. aquatica and D. glomerata contributed to a high rate of nitrate interception by these species and low leachate losses. The L . multifloru m/ A. capillaris mixture was intermediate between the two species for most of the variables measured.     

Ground cover, photosynthetic rate and tuber yield of potato (Solanum tuberosum L.) crops from seed tubers with different physiological age modified by foliar applications of plant growth regulators

Summary The modifying effects of applying the plant growth regulators (PGRs) benzylaminopurine (BAP), gibberellic acid (GA₃) and BAP+GA₃ on physiological age were studied. Two experiments with two cultivars, differing in rate of physiological ageing (medium-early Pampeana, medium-late Huinkul) and two storage systems were performed during 1988/89 and 1989/90 in two different potato areas of Argentina. In both seasons seed tubers stored in heaps reached an advanced physiological age at planting, compared with tubers from the cold store. Seed tubers of cv. Pampeana were older than those of Huinkul. compared with control crops, those sprayed with BAP maintained ground cover and photosynthesis for longer, and those sprayed with GA₃ for a shorter period. Consequently tuber yield was decreased by GA₃ in 1988/89, but in 1989/90 all crops treated with PGRs outyielded the control. BAP could overcome effects of advanced physiological age on crop senescence and tuber yield. CIC BA This work was carried out with a research grant from CAFPTA (249/89).