玉米籽粒发育性状的遗传及与产量性状关系的研究

在含有 BA(6-苄基腺嘌呤)2.5毫克/升、NAA(萘乙酸)0.1毫克/升的 MS 培养基上诱导苎麻(Boehmeria niveaL.)试管苗未离体叶面生芽。比较了不同激素的效果,在诱导芽形成中 BA 优于 ZT(玉米素)和 KT(激动素)。在叶脉处,特别是在中脉维管束的薄壁细胞容易分裂和脱分化,且具有较强的分化能力。芽在叶面上发生有一定的规律,并对叶面     

缺苗断垄对机播夏玉米产量及产量构成因素的影响

为探讨缺苗断垄对机械化播种夏玉米的影响,在大田状态下设置2个品种不同缺苗断垄程度进行试验,并对缺苗级别、断垄级别、籽粒产量及其产量构成因素进行统计分析。结果表明,缺苗断垄引起产量降低的主要原因是株数和穗数减少,随着缺苗断垄级别的增加,株数穗数逐渐减少,产量逐渐降低。产量降低幅度小于缺苗断垄级别的增加幅度,在缺苗断垄率为10.00%～40.00%的情况下,产量降低幅度为3.60%～28.51%;在缺苗断垄率相同的情况下,鲁单981比郑单958的减产幅度大些。     

Agronomic Performance and Nutrient Concentration of Maize (Zea mays L.) as Influenced by Nitrogen Fertilization and Plant Density

A field experiment was conducted at Samaru, Nigeria to study the influence of nitrogen fertilization and plant density on the agronomic performance and nutrient concentration of maize ( Zea mays L.). The treatments were factorial combinations of five nitrogen fertilization rates (0, 50, 100, 150 and 200 kg N/ha) and three plant density levels (25000, 50000 and 75000 plants/ha).
Nitrogen fertilization up to 150 kg N/ha enhanced grain and stover yields and increased kernel number and weight up to 100 kg N/ha. Nitrogen supply also increased maize ear length. Increased N fertilization rates increased concentrations of N, K and Mg but had no effect on P and Ca concentrations. Higher grain yield was closely associated with higher N concentration in ear leaf; with 0.1 % change in N concentration causing 177 kg/ha change in grain yield. Increased plant density increased stover yield up to 50 or 75 thousand plants/ha but depressed kernel number and weight and ear length.     

渍涝胁迫对玉米生理生化的影响研究进展

渍涝胁迫是玉米生产中重要的非生物逆境之一,对玉米的生长发育造成危害,严重制约了玉米产量的提升。因此回顾了人们针对渍涝胁迫对玉米生理生化的影响及其适应机制的研究,介绍了人们从栽培耕作和遗传育种两个角度提高玉米抗渍涝能力的研究现状,并展望了未来玉米抗渍涝胁迫的重点研究方向。     

苗期水分胁迫对玉米根系生长杂种优势的影响

以玉米杂交种高油115及其亲本(母本220、父本1145) 为材料, 研究了玉米苗期根系生长的杂种优势及水分胁迫的影响。试验设水分充足、轻度干旱和严重干旱3个处理, 田间持水量分别为75%、55%和35%。结果表明, 在水分充足条件下, 根长(RL)、根表面积(SA)和根干重(RDW)均表现出不同程度的中亲优势和超亲优势, 轻度干旱显著降低了各指标的杂种优势, 而严重干旱则完全抑制了根系生长的杂种优势。根系各性状中, RL的杂种优势最强。在水分充足条件下, 与中等根(根直径0.25~0.45 mm)和粗根(根直径>0.45 mm)相比, 细根(根直径0.05~0.20 mm)的RL、SA及其占总根系比例的杂种优势最高。轻度干旱胁迫虽然降低了根系的杂种优势, 但RL、SA仍表现出显著的超亲优势和中亲优势(粗根的超亲优势除外)。此外, 只有细根RL和SA占总根系的比例在轻度干旱胁迫下具有显著的中亲优势, 说明杂交种可以通过生成较亲本更高比例的细根来抵抗轻度干旱胁迫。     

Resin-Extractable Phosphorus, Vanadium, Calcium and Magnesium as Factors in Maize (Zea mays L.) Yield

Resin extraction of soil permits evaluation of ratios of readily extracted elements and correlation of concentrations obtained with crop yield. This information provides guidance on potential genotype selection and fertility management. Two hybrids of maize ( Zea mays L.), designated 2292 and 3895, were grown annually in rotational succession on a 3.2-ha site with soybean ( Glycine max L.) and wheat ( Triticum aestivum L.). Soil samples (0–15 cm) were extracted with ion-exchange resins and extracts were analysed with inductively coupled plasma. Data were regressed against crop yield using stepwise multiple correlation methods. Each hybrid was sensitive to unique combinations of extracted chemistries. Both hybrids of maize were sensitive to the resin-extractable V : (V + P) molar ratio and potential losses of ≥ 20 % were indicated as the ratio approached 0.2. A positive response to the Mg : (Mg + Ca) resin-extractable ratio was noted for maize hybrid 2292 in each of three successive years. Changes of yield potentials associated with the Mg : (Mg + Ca) ratio for hybrid 2292 ranged from none to ≥ 20 % as the ratio ranged from 0.2 to about 0.8. The results indicate that, in the presence of large extractable concentrations of competing or inhibitory ions, different approaches to nutrient management in the form of fertilizer nutrient analysis, application, and genotype selection are needed to overcome effects of competing ions.     

Water Stress and N Fertilization Effects on Yield and Smut Disease Incidence in Corn (Zea mays L.)

A 2-year field experiment was conducted in sandy clay loam soil to quantify the single and combined effects of N fertilization and water stress (WS) treatments on yield and smut incidence in corn. The plants were supplied with two different N rates (225 and 300 kg ha⁻¹) and subjected to 2 weeks' water stress, imposed at vegetative (Vg), tasselling (Ta), silking (Si) and grain-filling (Gf) growth stages. In addition, a control treatment (Cr) was also included to allow the plants to grow under unstressed conditions. The plants were artificially infected two times with Ustilago maydis spores and smut severity was recorded. At harvest, grain yield (GY), total biomass (Tbm) and harvest index (HI) were determined in smut-free (0) and smutted plants (S). Results have shown that GY₀ and GY_s were markedly reduced when the plants were subjected to WS. The depressing effects of WS were, comparatively, high at Gf. intermediate at Ta and Si. and low at Vg growth stages. Smut index (SI) data revealed that severity was higher on plants stressed at Gf than those exposed to WS at Vg, Ta or Si stages. Considerable variations were detected on Tbm and HI of smut-free and smutted plants, indicating the detrimental effect of plant water deficit at Gf stage. The combined effect of WS and N on Tbm_s showed mixed results, ranging from positive response for stressed plants at Si and Gf to little response at Cr, Vg or Ta stages at higher N rate. Although the effect of WS exposure on RYL data was dominant at Gf, the rate of yield depression was relatively higher at Ta and Si than the other growth stages, because of water deficit effects. The results proved that N fertilization did not conclusively alleviate the depressing effect of WS on grain yield losses.     

不同地力水平下控释尿素对玉米物质生产及光合特性的影响

氮肥是玉米生产中最重要的增产要素之一。为探明不同地力水平下控释尿素对玉米物质生产及光合特性的影响, 采用盆栽试验研究了释放期不同的2种控释尿素(CRU₃₀, 释放期为30 d; CRU₆₀, 释放期为60 d)的作用效果, 以普通尿素为对照(U)。结果表明, 控释尿素处理显著增加玉米干物质积累量, 但释放期不同的控释尿素在不同地力水平下增产效果不同, 低地力水平下增产效果为CRU₃₀>CRU₆₀>U (P<0.05), CRU₃₀处理比施用普通尿素增产18.9%; 高地力水平下增产效果为CRU₆₀>CRU₃₀>U(P<0.05), CRU₆₀处理比U增产18.2%。与对照相比, 控释尿素使干物质向开花后分配比例增加, 氮肥偏生产力(PFPN)显著提高, 低地力时CRU₃₀最高, 而高地力时CRU₆₀最高。控释尿素处理的单株干物质积累量、穗粒数及千粒重显著增加, 主要因为生育中后期叶面积、光合速率、叶绿素及叶片氮含量维持较高水平。所以, 在等氮量做基肥一次性施入时, 低地力水平下施用释放期较短的控释尿素为宜, 而高地力水平下应施用释放期较长的控释尿素。     

普通玉米3个籽粒品质性状的配合力分析

以10个普通玉米自交系及其90个杂交组合在北京和山西2个地点的试验数据,分析了蛋白质、淀粉和油分含量的配合力效应。结果表明：(1) 各性状的一般配合力(GCA和特殊配合力(SCA) 均方极显著,且GCA均方大于SCA均方,说明它们在遗传上主要受加性基因效应的影响。(2) 杂交种的3个品质性状与亲本GCA平均值和SCA极显著正相关,但与亲本GCA均值间的相关性相对较强,其相关系数(r)分别为0.708**(蛋白质)、0.756**(淀粉)和0.772**(油分),表明提高亲本GCA对改良杂交种品质具有更大的作用。(3) 自交系BT1和E28的蛋白质含量GCA较高,掖478、昌7-2、掖107和48-2的淀粉含量GCA较高,E28、黄C和P138的油分含量GCA较高。     

Osmotic Adjustment in Maize (Zea mays L.): Changes with Ontogeny and its Relationship with Phenotypic Stability

Genetic variation for osmotic adjustment was examined in a group of maize hybrids during vegetative and silking stages. Estimates of osmotic adjustment were derived from measurements of leaf relative water content (RWC) and osmotic potential (Ψ o ) before day-break. The degree of adjustment was estimated from the In RWC/In Ψ o relationship. All cultivars examined showed some degree of osmotic adjustment and significant differences (P < 0.05) were found among them. The utility of measurements in early vegetative stages as a mean of selection for osmotic adjustment in silking stage was established. A positive tendency was observed between osmotic adjustment and phenotypic stability.     

夏玉米光合特性对氮素用量的反应

随氮素用量增加,植株下位叶、穗位叶和上位叶的光合速率（P_n）、叶绿素含量（Chl）、可溶蛋白含量（Pro）、Hill反应活性、Ca²⁺-ATPase活性、Mg²⁺-ATPase活性和PEPCase活性均不断增大。植株不同叶位Pn和Pro随生长进程均不断下降;不同叶位Chl的变化动态表现为下位叶在各测定时期之间差异较小、穗位叶和上位叶为单峰曲线。Hill反应活力、Ca²⁺-ATPase活性和Mg²⁺-ATPase活性的变化规律不同,随生长进程表现不断下降、单峰曲线和双峰曲线等不同形式。各叶位PEPCase活性均表现单峰曲线型变化。随氮素用量增加,叶源量、生物产量和籽粒产量不断增加。适量施氮具有全面改善玉米光合效率和内在生理、生化过程的作用。Ca²⁺-ATPase活性是影响光反应活力的重要因素;在叶片生长前中期,P_n受到PEPCase的影响较小,主要受到光反应活力和暗反应效率的调控。可采用叶源量作为玉米单株生产力的参考诊断指标。     

Effects of Thiourea, Thiamine and Ascorbic Acid on Growth and Yield of Maize (Zea mays L.)

Three chemicals, viz., thiourea, thiamine and ascorbic acid capable of potentiating-SH turnover, were tested with the objective to improve growth and productivity of maize. Thiourea was tested as seed-soaking and foliar-applied treatments as also in combination. Thiamine and ascorbic acid were tested as foliar sprays alone. Foliar treatments were applied at vegetative stage (30 and 45 days after sowing).
The results of the field experiment showed that seed soaking with thiourea (500 ppm) tended to improve grain yield (13.4 per cent over control), but improvement in biological yield was significant. However, seed soaking plus foliar treatment of thiourea significantly increased both biological and grain yields, besides causing significant improvement in leaf area index and number of green leaves plant^-1. The increase in grain yield ha^-1 was of the order of 34.6 per cent over control.
It was further noted that foliar sprays of thiourea (1000 ppm), thiamine (100 ppm) and ascorbic acid (100 ppm) significantly increased leaf area index, number of green leaves plant^-1 and biological yield ha^-1. These treatments also significantly increased grain yield ha^-1 by 40.6, 20.2 and 26.3 per cent, respectively over control. Improvement in maize yield with thiourea, thiamine and ascorbic acid treatments appeared to have resulted from increased photosynthetic efficiency and canopy photosynthesis on account of the biological activity of -SH group. It was also apparent that leaf senescence was delayed under the influence of these chemicals. It is therefore suggested that thiourea, thiamine and ascorbic acid are the potential bioregulators for improving photosynthetic efficiency and grain yield of maize and possibly other cereals, and that thiourea, a sulphydryl compound, holds considerable promise in this context.     

Effect of Salt Stress on the Salicylic Acid Synthesis in Young Maize (Zea mays L.) Plants

The effect of salt stress on salicylic acid (SA) synthesis was investigated parallel with the induction of antioxidant enzymes in young maize plants. Two-week-old maize plants grown in hydroponic solution were treated with 50 or 100 m m NaCl for 7 days. Antioxidant enzyme activities, and the SA and o -hydroxy-cinnamic acid (oHCA) levels were measured on the 3rd and 7th day of treatment and after 4 days of recovery. Ascorbate peroxidase activity increased in the leaves, but changes in guaiacol peroxidase activity only could be detected in the roots after 7 days. Glutathione reductase activity increased both in the leaves and in the roots after the 3rd day of 100 m m NaCl treatment. Free SA only increased during recovery in the leaves and roots. In the leaves of plants treated with 100 m m NaCl, a slight increase was observed in the free oHCA level, which rose dramatically after recovery, while in the roots an increase could only be seen after recovery. These results suggest that oHCA may serve not only as a precursor of SA but may also have an antioxidant role during salt stress and recovery.     

黑龙港流域玉米不同生育阶段气象因子对产量性状的影响

明确玉米生长发育不同阶段气象因子与产量的关系,有助于确定区域最适播期。通过调整播期来改变玉米生育期内气候条件,对于抵御阶段性不良气象因子的胁迫并最终实现高产目标有重要意义。本研究以郑单958为试验材料,于2009年至2010年在黑龙港地区中国农业大学吴桥试验站进行分期播种试验,分析产量及产量构成因素与不同阶段气象因子的关系。结果显示: (1)由于年际间气象条件的差异,产量及其构成因素并非简单地随播期变化而变化。(2)在试验设定的高密度条件下,产量提升主要受千粒重的制约,穗粒数次之。(3)产量性状与不同生育阶段的多个气象因子显著相关。穗期、抽雄吐丝阶段的光照条件对穗粒数以及产量有影响;苗期、营养生长阶段气温日较差与产量显著正相关;抽雄吐丝前后的温度条件影响穗粒数;生育期总降水量影响穗粒数和千粒重的提升。该地区在调整播种时间、改进栽培措施时,上述关系应是考虑的重点。生产中可适当早播晚收,选用适宜的中晚熟品种,既可避开生育前期及籽粒形成阶段不利气象因子的影响,又可延长籽粒灌浆时间,充分利用该地区生育后期丰富的光热资源。     

Effects of Nitrogen Fertilization on Yield and Yield Components of Tropical Maize Cultivars

Six tropical maize cultivars were grown in Thailand on a reddish brown latosol under three nitrogen regimes, 0, 40 and 80 kg N/ha. With regard to biomass and economic yield several "cultivar by N fertilization response types" were discriminated. The low fertilization type was represented by an early and a late maturing cultivar, both yielded comparatively well without additional N and responded little to N fertilization. An opaque 2 cultivar and a commercial hybrid belonged to the high fertilization type, they had a very low yield without N and needed high N fertilization to produce a high yield. The other genotypes were of an intermediate type with comparatively high yields without N, a considerable increase in yield at 40 kg N/ha and some increase from 40 to 80 kg N/ha. Genotypic variability for the harvest index was high but values were at the usual low level of tropical cultivars. High fertilization types reacted to an additional N supply with a marked increase in kernel number/plant and little increase in kernel weight. The early maturing low fertilization type reacted quite the opposite way. All other genotypes predominantly increased kernel number from 0 to 40 kg N/ha and kernel weight from 40 to 80 kg N/ha.     

低磷胁迫对磷不同利用效率玉米叶绿素荧光参数的影响

盆栽试验在高磷（+P）和低磷(－P)条件下,于四叶期测定了磷高效利用型玉米KH5和磷低效利用型玉米齐201的老叶（第2叶）和新展开叶的（第4叶）叶绿素荧光诱导动力学参数。结果表明,低磷处理使玉米叶片PSⅡ关闭程度增加、光能转换和电子传递效率降低,过剩激发能增加。基因型间比较,磷高效利用型玉米的光能转换和电子传递效率     

高温对玉米籽粒淀粉合成及产量的影响

以农大108和山农3号为试材，采用田间生长箱增温的方法，研究了不同生育时期高温对玉米淀粉合成及产量的影响。结果表明，第1期（出苗后0~28 d）高温处理对玉米的产量没有显著影响，第2期（出苗后29~57 d）和第3期（出苗后58~86 d）高温处理显著降低玉米产量，以第2期对产量的影响最大；同时高温降低1,6-二磷酸酯酶（FBPase）、磷酸蔗糖合成酶（SPS）、蔗糖合成酶（SS）和腺苷二磷酸葡萄糖焦磷酸化酶（ADPGPPase）的活性，且以第2期降低的幅度最大；籽粒淀粉含量，以第1期高温处理高于对照，其他2个处理显著低于对照。     

Direct and Residual Contributions of Symbiotic Nitrogen Fixation by Legumes to the Yield and Nitrogen Uptake of Maize (Zea mays L.) in the Nigerian Savannah

Field experiments were conducted to determine the direct and residual contributions of legumes to the yield and nitrogen (N) uptake of maize during the wet seasons of 1994 and 1995 at the University Farm, Abubakar Tafawa Balewa University, Bauchi, Nigeria, located in the Northern Guinea savannah of Nigeria. Nodulating soybean, lablab, green gram and black gram contributed to the yield and N uptake of maize either intercropped with the legumes or grown after legumes as a sole crop. Direct transfer of N from the nodulating soybean, lablab, green gram and black gram to the intercropped maize was 24.9–28.1, 23.8–29.2, 19.7–22.1 and 18.4–18.6 kg N ha^–1, respectively. However, the transfer of residual N from these legumes to the succeeding maize crop was 18.4–20.0, 19.5–29.9, 12.0–13.7 and 9.3–10.3 kg N ha^–1, respectively. Four years of continuous lablab cropping resulted in yields and N uptake of the succeeding maize crop grown without fertilizer N that were comparable to the yields and N uptake of the succeeding maize crop supplied with 40–45 kg N ha^–1 and grown after 4 years of continuous sorghum cropping. It may therefore be concluded that nodulating soybean, lablab, green gram and black gram may be either intercropped or grown in rotation with cereals in order to economize the use of fertilizer N for maize production in the Nigerian savannah.     

The Influence of Plant Water Stress on Net Photosynthesis and Leaf Area of Two Maize (Zea mays L.) Cultivars

The effect of plant water stress on net photosynthesis and leaf growth were investigated in order to determine to what extent leaf water potential during vegetative growth and silking affects maize development.
Two commercial maize hybrids grown in pots in a glasshouse were subjected to leaf water potentials of -1300 and -1700 kPa during the eighth leaf stage and during silking to -1700 and -2300 kPa to previously unstressed, moderately and severely stressed plants. The effect of stress on inhibiting CO₂ uptake rates and leaf areas, as well as the recovery after alleviating stress, were compared to that of unstressed plants.
No substantial differences in CO₂ uptake rates were found between medium and long seasoned cultivars. The CO₂ uptake rates per unit leaf area decreased to negative values under both moderate and severe stress conditions during both growth stages. During silking, the recovery of CO₂ uptake rate was much lower than during the eight leaf stage. Leaf area decreased proportionally with increased stress but did not recover after alleviating stress on plants stressed during both the eighth leaf and silking stages.     

玉米生长素响应因子家族基因的表达模式分析

生长素响应因子(auxin response factor,ARF)是一类重要的转录因子,通过特异性地结合生长素响应元件调节下游靶基因的转录,参与诸多植物生长发育过程的调控。玉米中有许多ARF家族基因,但其表达模式有待深入研究。本研究分析了玉米ARF家族基因在不同组织器官中的表达,发现除ARF10、ARF16和ARF34组成型表达外,其余32个ARF基因的表达水平在生殖器官中要明显高于营养器官。对ARF基因启动子区的顺式作用元件分析显示,28个ARF基因的启动子区含有逆境胁迫相关顺式元件,实时定量PCR分析结果显示,多个ARF基因分别响应冷、热、盐和渗透胁迫。研究结果不仅暗示了ARF家族基因在玉米生殖生长和非生物逆境胁迫响应中的重要性,也为全面解析ARF基因在玉米中的生物学功能提供有用信息。