Barley/pea mixtures as cover crops for grass re-seeds

Substantial annual fluctuations in the performance of arable silage mixtures, comprising Minerva forage pea and Goldmarker barley, were observed from ten annual sowings between 1977 and 1986, Total dry matter (DM) yield varied between 2·5 and 11·5 t ha⁻¹. Dry matter content also ranged widely from 10·3% to 20·0% and there was a large variation in the proportion of peas in the DM (0·44 to 0·90). Almost 80% of this yield variation was due to the fluctuation in pea performance and over 60% of all yield variation was related to the variation in sunshine hours and air temperature.
In 1983 and 1984 differences in seed rate of between 20 and 140 kg ha⁻¹ of Minerva and 80 and 160 kg ha⁻¹ of Goldmarker influenced the forage composition at harvest, with the pea component capable of outgrowing and suppressing the other species when sown at over 80 kg ha⁻¹. Very high yields of peas were associated with increasing stress on the undersown grass re-seed and were considered undesirable.
It was concluded that sowing rates of between 120 and 160 kg ha⁻¹ for barley and a maximum of 60 kg ha⁻¹ for peas would, in most years, provide the best compromise between attaining good arable silage yields and avoiding excessive dangers of damage of undersown grass re-seeds.     

Sulphur concentration of soils and plants and its requirement for ruminants in the Inner Mongolia steppe of China

The sulphur status of four soils and 122 forage plants from the Inner Mongolia steppe was determined. The organic sulphur concentration ranged from 17 μg g^–1 in the 0·2–0·4 m soil layer to 397 μg g^–1 in the topsoil. The mean sulphate-S concentrations were <10 μg g^–1; greater concentrations were found only in the chernozem soil. Biomass-S accounted for 0·018–0·028 of total S in four soils. Three-quarters of plant samples examined had <1·6 g kg^–1 S, and >0·80 of them had an N:S ratio >14:1. More than 0·80 of the plants were deficient in S. There was a close relationship between plant-available soil sulphur concentrations and total plant sulphur concentrations. It was concluded that sulphur deficiency is widespread in the Inner Mongolia steppe and that sulphur fertilizer requirements should be evaluated.     

Nitrate leaching from cut grassland as affected by the substitution of slurry with nitrogen mineral fertilizer on two soil types

A field experiment was conducted to find out whether there is any difference in risk of N leaching to groundwater when cattle slurry and/or mineral fertilizer-N was applied to cut grassland. The experiment was carried out over two consecutive years on two sites (one with a relatively wet sandy soil and one with a relatively dry sandy soil). Treatments were mineral fertilizer-N at annual rates of 0–510 kg N ha⁻¹ year⁻¹ and combinations of sod-injected cattle slurry (85, 170, 250 and 335 kg N ha⁻¹ year⁻¹) and mineral fertilizer-N (289, 238, 190 and 139 kg N ha⁻¹ year⁻¹). Yield responses indicated that in the short run, 0·44–0·88 (average 0·60) of the slurry-N was as available as mineral fertilizer-N. The total N input from mineral fertilizer and slurry was a worse predictor of nitrate leaching (     0·11) than the N surplus (i.e. the difference between total N input and harvested N) (     0·60). The effective N surplus, based on the difference between the summed inputs of the plant-available N and harvested N, proved to be the best indicator of leaching (     0·86). Annual N application rates of up to 340 kg plant-available N ha⁻¹ complied with the target nitrate concentration in groundwater of 11·3 mg N L⁻¹ set by the European Union in both years on the wet sandy soil, whereas on the dry sandy soil none of the treatments did.     

Effects of different rates and timing of application of nitrogen as slurry and mineral fertilizer on yield of herbage and nitrate-leaching potential of a maize/Italian ryegrass cropping system in north-west Portugal

Efficient use of cattle-slurry to avoid nitrogen (N) leaching and other losses is important in designing intensive dairy systems to minimize pollution of air and water. The response in dry-matter (DM) yield of herbage and nitrate-leaching potential to different rates and timing of application of N as cattle slurry and/or mineral fertilizer in a double-cropping system producing maize ( Zea mays L.) silage and Italian ryegrass ( Lolium multiflorum Lam.) was investigated in north-west Portugal. Nine treatments with different rates and combinations of cattle slurry, and with or without mineral-N fertilizer, applied at sowing and as a top-dressing to both crops, were tested and measurements were made of DM yield of herbage, N concentration of herbage, uptake of N by herbage and amounts of residual soil nitrate-N to a depth of 1 m, in a 3-year experiment. Regression analysis showed that the application of 150 and 100 kg of available N ha⁻¹ to maize and Italian ryegrass, respectively, resulted in 0·95 of maximum DM yields of herbage and 0·90 of maximum N uptake by herbage. Residual amounts of nitrate-N in soil after maize ranged from 48 to 278 kg N ha⁻¹ with an exponential increase in response to the amount of N applied; there were higher values of nitrate-leaching potential when mineral-N fertilizer was applied. The results suggest that it is possible in highly productive maize/Italian ryegrass systems to obtain high DM yields of herbage for maize silage and Italian ryegrass herbage with minimal leaching losses by using slurry exclusively at annual rates of up to 250 kg available N ha⁻¹ (equivalent to 480 kg total N ha⁻¹) in three applications.     

Regulating the content of white clover in mixed swards using grass-suppressing herbicides

A preliminary investigation evaluated six grass-suppressing herbicides applied on two occasions in late winter to a predominantly ryegrass ley containing only 15% ground cover of white clover. Substantial increases in clover growth, estimated visually, and flower head numbers per unit area were recorded in the first summer after treatment with 2·8 kg ha^-1 carbetamide, 0·8 kg ha^-1 propyzamide and 0·6 kg ha^-1 paraquat. To achieve these increases, visual estimates suggested that spring growth of grass was reduced by 40–80%. However, grass growth recovered fully by mid-summer on the majority of the treatments.
The following year five of the herbicides were compared in a field experiment. Dry matter (DM) and nitrogen (N) assessments of the grass and legume components were made at three harvests in the first growing season and a single harvest in the second year. Carbetamide, paraquat and, especially, propyzamide increased the proportion of clover in the DM (to 89% in the case of 1·2 kg ha^-1 propyzamide); in general, using herbicides to raise clover contents above 20% lead to reductions in spring grass growth of about 70%. However, such reduction was offset by subsequent increased growth so that total annual yields were largely unaffected. The increased legume content resulted in an increased N concentration in both grass and legume components, measured in the second summer. At this time, the greatest increase in total N yield (up to 35%) was recorded from 0·6 kg ha^-1 propyzamide. Potential uses to achieve legume dominance by grass-suppression are suggested and the needs for further research are outlined.     

Cell wall degrading enzymes for silage. 2. Aerobic stability of enzyme-treated laboratory silages

The effects of two commercial cellulase/hemicellulase enzymes derived from Trichoderma reesei on silage fermentation and aerobic stability were investigated in three laboratory-scale experiments. In Experiment 1, perennial ryegrass ( Lolium perenne ) was treated with enzyme A at the rates of 0. 0·125, 0·250, 0·500 and 0·750 cm³ kg^-1. In Experiment 2, Italian ryegrass ( Lolium multiflorum ) was treated with the same enzyme at the rates of 0, 0·250 and 0·500 cm³ kg^-1 and with 85% formic acid (3·5 cm³ kg^-1). In Experiment 3, perennial ryegrass was ensiled untreated, with enzyme A (0·250 and 0·500 cm³ kg^-1) and with 0·200 and 0·400 cm³ kg^-1 enzyme B which also contained glucose oxidase. All silages were well preserved. Aerobic deterioration was related to numbers of lactate assimilating yeasts. In Experiment 1, all silages were stable over a 4-day period. In Experiment 2, enzyme A treatment delayed the temperature rise compared with the untreated control ( P <0·05), whereas the formic acid-treated silage was stable. In Experiment 3, the temperature rise in the silage treated with the higher level of enzyme B occurred one day later than in the other silages ( P <0·05).     

Silage and milk production: studies with diets containing white clover silage

In three separate feeding experiments using a total of twenty-six individually-housed Ayrshire cows, three wilted silages made from Blanca white clover were offered ad libitum with either different supplements or different proportions of grass silage. The clover silages contained 680 g white clover kg⁻¹ on a DM basis, and had a mean DM concentration of 263 g kg⁻¹ with 231 g CP kg⁻¹ DM and 91 g ammonia-N kg⁻¹ N. The pH values averaged 4·16 and the DOMD concentrations 611 g kg⁻¹. In experiment 1 the daily intake of clover silage given alone was 15.2 kg DM per cow, i.e. 30·1 g kg⁻¹ live weight, and decreased by 0·76 kg DM kg⁻¹ barley DM and by 0·66 kg DM kg⁻¹barley plus soybean meal DM when these feeds were offered as supplements. Milk yield and fat concentration were higher on the supplement treatments than on the clover silage-only treatment. In experiments 2 and 3 the intakes of silage and total DM increased as the weight of clover in the diet increased from 0 to 700 g kg⁻¹ with parallel increases in milk yield. The effects on milk composition were small and generally non-significant. Although white clover silages with excellent fermentations were made, it is concluded that the main role of white clover in a silage system will be in mixed swards with grass to reduce the input of fertilizer N and to increase the voluntary intake of silage.     

Dry-matter yield and herbage quality of a perennial ryegrass/white clover sward in a rotational grazing and cutting system

The expected reduction in the use of fertilizer nitrogen (N) on grassland in the Netherlands has led to renewed interest in white clover. Therefore, the performance of a newly sown perennial ryegrass/white clover sward on clay soil was assessed during 4 consecutive years. The experiment consisted of all combinations of two defoliation systems, i.e. one or two silage cuts per year (S1, S2), spring N application rate, i.e. 0 or 50 kg ha⁻¹ year⁻¹ (N₀, N₅₀), and the management system, i.e. rotational grazing and cutting, or cutting only (RGC, CO). The overall mean white clover cover was 30%. All treatments affected white clover cover, which was 8% higher with S2 than with S1, 6% higher with N₀ than with N₅₀ and 12% higher with CO than with RGC. The overall mean annual dry-matter (DM) yield (13·1 t ha⁻¹ year⁻¹) was significantly affected only by the management system: in two relatively wetter years, the annual DM yield was 1·19 t ha⁻¹ higher with RGC than with CO, whereas there was no difference in two relatively drier years. Nitrogen application increased the DM yield in the first cut by 7·0 kg kg⁻¹ N applied, but had no significant effect on the annual DM yield. Herbage quality was not affected by the experimental treatments. The average in vitro organic matter digestibility was 0.801, and the average crude protein content was 193 g kg⁻¹ DM. With the expected reduction in the use of fertilizer N, perennial ryegrass/white clover swards should be seriously considered as an alternative option to perennial ryegrass swards on these clay soils.     

The potential of an all-grass diet for the late-winter calving dairy cow

In a trial lasting 3 years at Jealott's Hill and 2 years at Ravenscroft Hall, a total of 73 January- March-calving Friesian x Holstein cows and heifers were given a diet consisting almost exclusively of ensiled and grazed ryegrass for an entire lactation to establish the level of milk production which grass alone can support. From calving to turnout in April/May (average duration 87 days), the animals were given high digestibility (DOMD = 678g kg-¹ DM) ryegrass silage ad libitum. After turnout, they grazed ryegrass swards until housing in autumn. Thereafter, the cattle were given ryegrass silage of lower digestibility (DOMD = 600–620 g kg-¹ DM) for the remainder of the lactation and during the dry period.
Mean silage dry matter intakes from calving to turnout were 13–2 kg day-¹ at Jealott's Hill and 11·5 kg day-¹ at Ravenscroft Hall. Average milk yields were 21-1 kg day-¹ for cows and 16-1 kg day-¹ for heifers with 39-2g fat kg-¹, 29-6g protein kg-¹ and 47–9 g lactose kg-¹. Over the complete lactation, average milk yields were 4680 kg and 4006 kg for the cows and heifers, respectively, with 39-4 g fat kg-¹, 31·4 g protein kg-¹ and 46·9 g lactose kg-¹. Animal health and fertility were satisfactory. The results demonstrated the value of high quality grazed and ensiled ryegrass and provided a measure of milk production from grass only.     

The effect of rate and timing of phosphate fertilizer on the yield and phosphate off take of grass grown for silage at moderate to high levels of soil phosphorus

A series of twenty-one trials was undertaken during 1985–1988 to investigate the effect on lowland silage crops of 0, 40 or 80 kg ha⁻¹ phosphate (P₂O₅) fertilizer applied as triple superphosphate (46% P₂O₅) in the autumn, spring or as a split application (spring and after first cut). All sites had moderate to high sodium bicarbonate-extractable soil phosphorus contents (18–34 mg P 1⁻¹ in air-dried soil).
Significant yield responses were obtained at eight of the forty-two individual cuts (two cuts per site). When meaned over all sites, spring or autumn applied phosphate increased dry matter yield at both first and second cut (mean total increase 0·32 t ha⁻¹), though the effect was significant only at the highest phosphate rate. The time of phosphate application had no overall effect on yield. Herbage P concentration and phosphate off take were significantly increased at both cuts by both rates of phosphate. Generally, the most recent application had a significantly greater effect than other timings. These findings indicate that DM yield responses to freshly available phosphate can occur on soils of moderate P status.     

Silage and milk production: comparisons between unwilted and wilted grass silages made with different additives

Four grass silages, all made in mid-July from second-harvest perennial ryegrass swards, were compared in a 16-week feeding experiment with twelve Ayrshire cows. Two silages were unwilted and two wilted. All the silages received formic acid ('Add-F') at the rate of 3 litres t^-1 either with formalin at the rate of 1 litre t^-1 or without formalin. The unwilted and wilted silages had mean dry matter (DM) concentrations of 200 and 243 g kg^-1, and in vitro D-values of 0·293 and 0·272 respectively. The silages were offered ad libitum plus 6 kg concentrates per cow per day. The daily intakes of unwilted and wilted silage DM were 10·2 and 9·2 kg per cow respectively on the formic acid treatment, and 10·2 and 9·2 kg on the formic acid + formalin treatment. The mean daily milk yield on the unwilted silage treatments was 19·2 kg per cow which was significantly higher than the yield of 17·2 kg per cow on the wilted silage treatments. The formalin had no significant effect on milk yield. The four silage treatments had small and non-significant effects on milk composition. It is concluded that the unwilted silages, which had excellent fermentation characteristics, were superior to the wilted silages as a feed for dairy cows.     

Short-term leaf surface adhesion of heavy metals following application of sewage sludge to grassland

In the short term, surface-applied, digested, liquid sewage sludge may remain on grass leaf surfaces, leading to enhanced direct intake of potentially toxic elements by stock. The purpose of this field experiment was to investigate metal adhesion to herbage from liquid sewage sludge applications. Liquid digested sludge was applied at 0, 55 or 110 m³ ha⁻¹ to an Agrostis capillaris and Holcus lanatus sward that had been cut to either 4 cm (short) or 13 cm (long). The application rate of metals was relatively low. One day after application (day 1), 8–13% of the sludge solids applied had adhered to the short grass treatment, accounting for 35–62% of the dry-matter (DM) yield. On the long grass, 20–42% of the sludge dry solids adhered, accounting for 46–47% of the herbage DM yield. The content of all heavy metals in and on the grass herbage (Cu, Fe, Ni, Pb, Zn, Cr, Cd) was significantly increased ( P < 0·05) at day 1. Cu concentrations in and on the grass decreased to 25 mg kg⁻¹ after 16–29 d, Fe took 33–45 d to decline to 1000 mg kg⁻¹ whereas Pb took 12–18 d to decline to 30 mg kg⁻¹. It took 36 d for Cd, 43 d for Fe, Cu and Ni, and 57 d for Zn and Pb to be not significantly ( P < 0·05) different from the control. Grass growth was the most significant factor( P < 0·05 for all treatments and metals) influencing the decline in grass metal concentration, explaining 65–96% of the variation. The cutting treatment, sludge application rate and metal species also significantly affected the extent and rate of reduction in metal contamination over time.     

A comparison of unwilted and wilted grass silages offered to beef cattle without and with monensin sodium

A randomized block experiment was conducted to compare unwilted and wilted grass silages and the effects of the feed additive monensin sodium on the silage intake and performance of finishing beef cattle. Two regrowths from a predominantly perennial ryegrass (cv. S24) sward were ensiled either without wilting or after field wilting for 3 d (dry matter (DM) concentrations 161 and 266 g kg⁻¹ respectively). Both silages were treated with formic acid (2·6 and 30 litre t⁻¹ respectively) and were well preserved. The silages were offered ad libitum to forty-eight Charolais-cross cattle (thirty-two steers and sixteen heifers, mean initial live weight 351 kg) for 145 d. All animals received 2·2 kg concentrates per head daily and half of those on each silage treatment received in addition 200 mg monensin sodium per head daily. Silage DM intake was 5.04, 504. 5·48, 5·63 ± 0.134 kg d^−l; fasted liveweight gain was 0·69, 0·77. 0·64 and 0·73 ± 0.033 kg d^−l and carcass gain was 0·47, 0·50, 0·40 and 0·45 ± 0·020 kg d⁻¹ for the unwilted silage without and with monensin and the wilted silage without and with monensin respectively. It is concluded that wilting grass of low DM concentration for 3 d prior to ensiling reduced the performance of finishing beef cattle below that obtained from well-preserved unwilted silage in spite of a higher DM intake being achieved with the wilted silage. The inclusion of monensin sodium in a silage-based diet increased performance without significantly affecting feed intake.     

Comparison of a straw/concentrate mixture with silage as a buffer feed for grazing dairy cows

Over a 24-week period during the 1986 summer, three groups of January- to March-calving dairy cows were either grazed conventionally (G) or grazed between morning and afternoon milkings and housed overnight and offered grass silage (Si) or a straw/concentrate mixture (St) ad libitum. The straw/concentrate mixture contained proportionately, 0-33 long barley straw, 0·28 ground barley, 0·12 soya bean meal, 0·25 molaferm and 0·22 minerals. The metabolizable energy (ME) and crude protein (CP) contents of the silage fed in weeks 1-8 and weeks 9-24 were 9-5 and 10⁻⁶ MJ kg MD⁻¹ and 160 and 191 g kg DM⁻¹ respectively. The straw mix had an ME content of 10⁻¹ MJ kg DM⁻¹ and CP content of 134 g kg DM⁻¹. Partial storage feeding with silage or a straw/concentrate mixture led to a decrease in estimated herbage DM intake. The feeding of the straw/concentrate mixture increased total DM intake, but the estimated total ME intake was similar for treatments G and St. The intakes (kg DM d⁻¹) for treatments G, Si and St were respectively, herbage 11·7,6·8,4·1; total l3·5,13·6,15·0; total ME intake (MJd⁻¹) 163, 155, 163.
Animal performance was, for treatments G, Si and St respectively: milk yield (kg d⁻¹) 19·2, 17·5, 19·1 (s.e.d. 0-87); milk fat content (g kg⁻¹) 36·9, 37·6, 37.1 (s.e.d. 1.22); milk protein content (g kg⁻¹) 35·3, 32·9, 33·4 (s.e.d. 0·76).     

The effect of fertilizer sulphur on grass production for silage

The effects of rate and pattern of fertilizer S application on herbage production from Perennial ryegrass-dominant swards at six potentially S-deficient sites in south-west Scotland were measured under a silage cutting regime (3 cuts year-1) with high fertilizer N application (300 kg ha⁻¹ year-1). Fertilizer S (as gypsum) was applied at annual rates from 0 to 48 kg ha⁻¹ as single spring applications before the first cut or split before each of the three cuts. The residual effect of S in the year following its application was assessed at one site.
Applied S increased total DM production at four of the six sites. These increases were restricted to the second and/or third cuts and were generally in the range 10-30% greater than where no S was applied. Timing of S application was not important in influencing annual DM yield. The residual effect of gypsum in the year following application was small.
The supply of available soil S is limiting herbage production in certain areas and soil types of south-west Scotland. Areas of S deficiency are likely to increase in the future and need to be identified more precisely than they are at present.     

Enzymes as silage additives for grass–clover mixtures

Seven laboratory-scale experiments were carried out to study the effects of cellulases/hemicellulases on silage fermentation of herbage from mixed swards of timothy ( Phleum pratense ), meadow fescue ( Festuca pratensis ) and red clover ( Trifolium pretense ). Enzyme-treated silage (approximately 3500 HEC units kg⁻¹ grass) reached a low pH sooner, had lower end pH, contained less NH₃-N and more lactic acid than did the untreated silage ( P < 0·05). Applied with an inoculant, these effects were even stronger. With easily ensiled crops (experiments 1, 2, 5 and 6) preservation was first of all improved by inoculation of lactic acid bacteria; however, for the low-sugar crops (experiments 3, 4 and 7) enzyme treatment was more significant. The enzymes derived from Aspergillus spp. gave more acetic acid than the enzymes from Trichoderma reesei. The Trichoderma enzymes liberated 4·8 g WSC kg⁻¹ FM gamma-irradiated grass during 60 d at pH 4·3 ( P < 0·05). On average, for all silages enzyme treatment increased the sum of residual sugar and fermentation products by 3·7 g kg⁻¹ FM (21 g kg⁻¹ DM) compared with the silages not treated with enzymes ( P < 0·001). Enzyme treatment increased the instantly degradable part of the feed, but total in sacco and in vitro digestibilities were not affected.     

Assessment of contrasting perennial ryegrasses, with and without white clover, under continuous sheep stocking in the uplands. 3. Herbage production, quality and intake

Herbage characteristics were monitored over 3 years (1985–1987) in three perennial ryegrass ( Lolium perenne L.) varieties as grass/only (200 kg N ha⁻¹) and grass/clover ( Trifolium repens L.) (75 kg N ha⁻¹) swards when continuously stocked with sheep. Although mean total annual herbage production was similar from the grass varieties, growth of Aurora, a very early-flowering variety, was higher than that of Aberystwyth S23 and Meltra (tetraploid) late-flowering types in spring. Herbage production from grass-only swards was 15% higher than from grass/clover swards.
In vitro organic matter digestibility (OMD) of Aurora and Meltra was 34 g kg ⁻¹ and 26 g kg ⁻¹ higher ( P < 0·01) than that of S23. Averaged over varieties, the OMD of grass/clover was 25 g kg ⁻¹ higher ( P < 0·05) than grass-only during the post-weaning period. Individual lamb digestible organic matter intake (DOMI) was 22% higher ( P < 0·05) on grass/clover than on grass-only during this period. Water-soluble carbohydrates content of Aurora was 42% higher ( P < 0·001) than that of both Meltra and S23.
Herbage characteristics were related to lamb performance reported previously. Lamb production was positively correlated with DOMI. However, the magnitude of differences in lamb performance between treatments was much greater than the extent of differences detected in herbage characteristics.     

The influence of surface and sub-surface application methods for pig slurry on herbage yields and nitrogen recovery

Experiments were conducted on a grassland site at Wrest Park, Silsoe, Bedfordshire between 1987 and 1989, to compare herbage yields from slurry applied by deep and shallow injection, low trajectory and conventional vacuum tanker methods. Slurry application rates for all spreaders were calibrated at 86 ± 5 t ha^-1, an equivalent of c. 200 kg NH₄⁺-N ha^-1 applied in autumn or in spring.
As expected, herbage yields following spring applications were higher than from autumn applications, with average mineral fertilizer equivalents of 122 and 89 kg N ha^-1 respectively. Yields from the conventional and low trajectory spreaders showed no consistent differences. However, in both years, first cut yields from plots were significantly lower ( P < 0·05) where slurry had been injected than where surface applications had been used by an average of 0·7 t DM ha^-1. Subsequent cuts in 1988 demonstrated higher residual effects from injection so that annual total yields were similar from all slurry applications irrespective of spreader type.
Analysis of N content revealed high N levels in herbage from deep injection plots. Mean concentrations of N in the herbage, expressed as a percentage of the dry matter, were 1·43 for surface treatments and 1·79 for deep injection in 1988, and 1·84 for surface treatments, 2·13 for shallow injection and 2·68 for deep injection in 1989.     

Utilization of silage nitrogen in sheep and cows: amino acid composition of duodenal digesta and rumen microbes

Two experiments are reported. In the first experiment the amino acid compositions of rumen bacteria and protozoa isolated from sheep given a diet of grass silage were compared with those taken from sheep given a diet of hay and barley; four sheep were assigned to each diet. There were no significant differences (P<0·05) between diets in the amino acid compositions of the microbial fractions and bacterial contents of α-ɛ-diaminopimelic acid were also similar for both diets.
In the second experiment the digestion of a grass silage and barley diet (65:35; 135 g CP (kg DM)^-1) was studied using four non-lactating Ayrshire cows fitted with cannulae in the rumen and in the proximal duodenum. The mean N intake was 154 g d^-1 and the corresponding duodenal flow was 126 ± 10 g d^-1 indicating that 0·21±0·07 of the N intake was absorbed between the mouth and the duodenum. The mean rumen NH₃-N concentration was high, 297 g litre^-1, and the mean rate of microbial protein synthesis in the rumen was low, 17 g microbial N (kg OM apparently digested in the rumen)^-1. There was a low content of bacterial N in the non-ammonia N at the duodenum (mean proportion 0·52±005) and low concentrations of methionine and lysine in the duodenal digesta protein. The results are discussed in relation to previously published data on the digestion of silage diets in sheep and to the utilization of silage diets for milk production in the cow.
It is concluded that with silage diets the supply of methionine and lysine to the duodenum is likely to be low because of the low rates of microbial protein synthesis in the rumen and that in cows those two amino acids may be limiting for milk production.     

Cell wall degrading enzymes for silage. 1. The fermentation of enzyme-treated ryegrass in laboratory silos

The effects of two commercial cellulase/hemicellulase enzymes derived from Trichoderma reesei on silage fermentation were investigated in three laboratory-scale experiments. In Experiment 1, perennial ryegrass ( Lolium perenne ) was treated with enzyme A at the rates of 0, 0·125, 0·250, 0·500 and 0·750 cm³ kg^-1. In Experiment 2, Italian ryegrass ( Lolium multiflorum ) was treated with the same enzyme at the rates of 0, 0·250 and 0·500 cm³ kg^-1 and with 85% formic acid (3·5 cm³ kg^-1). In Experiment 3, perennial ryegrass was ensiled untreated, with enzyme A (0·250 and 0·500 cm³ kg^-1) and with 0·200 and 0·400 cm³ kg^-1 enzyme B which also contained glucose oxidase. All silages were well preserved. In general enzyme treatment reduced pH and the contents of ammonia nitrogen, neutral detergent fibre and acid detergent fibre, whereas the contents of water-soluble carbohydrates, acetic acid, lactic acid and ethanol were increased.