绵羊顺气穴插枝对唾液及血清淀粉酶活性的影响

顺气穴是反刍动物和猪仅见的穴位 ,有关插枝顺气穴影响动物胃肠功能活动和治疗胃肠疾病的报道较多 [1～ 6]。但插枝该穴位后影响有关酶方面的报道甚少 ,鉴此 ,我们测定了绵羊顺气穴插枝前后唾液和血清淀粉酶活性的变化 ,报道如下。1　材料与方法1 .1　试验动物及分组　青海大学农牧学院动物室饲养的临床健康绵羊 (1～ 4岁 ) 1 4只 ,试验组和对照组各 7只 ,试验期间动物的饲料、饮水、运动相对稳定。1 .2　试验器材　 72 1分光光度计 (上海第三分析仪器厂 ) ;自制的开口器 ;1 0～ 1 5cm鲜嫩的柳枝条以及实验室常规仪器。1 .3　穴位　口内上腭…     

顺气穴插枝术的解剖所见

继“顺气穴插枝治疗牛眼病400例的体会”一文在《中兽医医药杂志》1991年第1期刊登后,笔者又对顺气穴插枝术进行了新的探索,即作了解剖观察,现将情况作以介绍。解剖的方法是,选一屠宰的黄牛,宰后按常规方法在顺气穴插入枝条,然后顺枝条走向进行解剖,发现当枝条插入顺气穴约5cm 深,就在同侧鼻孔内露出,用手指在该鼻孔鼻纵沟内就能摸到枝条,两侧均一样。     

顺气穴插枝治疗渤海黑牛瘤胃臌胀

顺气穴插枝疗法是根据祖国传统医学概念,利用枝条在穴位的刺激作用,达到通络、调气血的目的,使患畜解除疾病,恢复健康.笔者多年来利用顺气穴插枝治疗渤海黑牛瘤胃臌胀126例,疗效显著,介绍如下:     

顺气穴插枝治疗牛胃肠病和眼病的实验报告

顺气穴插枝疗法是流传在民间的一种治疗牛病的针灸治疗方法。1957年农业部畜牧兽医总局编写的《兽医针灸汇编》,以及以后出版的《中兽医针灸学》、《兽医针灸手册》等书均有收载。据云:顺气穴有治疗感冒、牛臌胀、角膜炎等病,特别对牛臌胀有顺气作用,故名顺气穴。1974年以来,我们曾用顺气穴插枝治疗牛宿草不转(瘤胃食滞)7例,气胀(瘤胃臌胀)4例,     

顺气穴插枝疗法治疗牛瘤胃臌气

笔者自2003年以来,采用顺气穴插枝疗法治疗牛瘤胃臌气病16例,均获得了满意的效果,现介绍如下:     

顺气穴插枝对牛羊生理功能作用的实验初报

作者近年来,在兽医临床工作中,应用顺气穴插枝法,治疗牛胃肠病37例,取得了满意的效果。为了进一步探索其对畜体功能的影响及治病原理,进行了健康牛、羊22例的实验,通过自身插枝前后的体温、呼吸、心跳、瘤胃蠕动、瘤胃液、血液等12项指标的对比观察结果,插枝后有增强瘤胃蠕动和白血球总数增加的作用。     

猪顺气穴插枝对T淋巴细胞的影响

猪顺气穴插枝疗法对猪的感冒发热、胃肠炎、眼结膜炎等的疗效一般可达90％左右,但对其治病机理,目前研究尚少。我们用免疫学方法观察了插枝疗法对猪T淋巴细胞的影响。材料与方法 (一)试验动物 2～3月龄的临床健康幼猪45头,第一批20头,第二批25头;健康公绵羊1只。     

顺气穴插枝治猪病案例

<正>顺气穴插枝疗法治病猪,经临床和实验研究证明,既能调节机体免疫不足,使免疫低下的动物其免疫力增加,又能抑制过度的免疫反应。增强机体免疫功能的机理主要表现在调节特异免疫应答和非特异免疫两个方面。插枝疗法对特异免疫的调节。体现在插枝后能调节免疫蛋白的分泌合成,促进淋巴细胞的活化、增殖并发挥功能。顺气穴是猪传统针灸疗法的穴位,经笔者多年临床验证,可治猪呼吸系统(如急性支气管炎、咽炎、鼻炎等)和消化系统(如消化不良、食欲不     

顺气穴插枝治疗牛眼病400例的体会

牛顺气穴插枝术是中兽医传统医疗技术,民间兽医称之为“穿鞭花”,意思是用此法治疗牛眼鞭伤效果好。笔者沿用此法已20多年,治疗眼病400多例,除2例先天性角膜浑浊无效外,其余均获满意效果。近来,在一些杂志上看到不少关于牛顺气穴插枝法的报道,如《南阳畜牧兽医》1989年第2期上肖银婉的“榆树条串顺气穴鸭蛋油点眼治疗家畜角膜炎效果好”,陈义     

一起乳山羊急性瘤胃臌气的诊治

乳山羊瘤胃臌气是由于瘤胃和网胃内容物产生大量发酵气体而引起的疾病。2017年10月,我们接诊了一起乳山羊瘤胃膨气病例,根据患羊症状的轻缓,分别采用顺气穴插枝疗法、瘤胃穿刺和中西兽医药物治疗等方法处理,3头患羊全部治愈。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.