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1.
Absorption of cysticerci in cattle after treatment of taenia saginata cysticercosis with praziquantel 总被引:1,自引:0,他引:1
Two groups of calves aged 10 to 15 weeks were orally infected with either 6000 or 30,000 Taenia saginata eggs. Three to four months later they were treated with praziquantel, three doses of 50 mg kg-1 on three consecutive days. All the cysticerci were killed by the drug and most were absorbed by between six and nine months after treatment. From then on there was little further absorption and residual calcified cysts were still found in the carcases up to 24 months after drug treatment. These residual calcified cysts were particularly common in the heart muscles and would probably have been detected at meat inspection. 相似文献
2.
Zbigniew Pawłowski Bronisław Kozakiewicz Henryk Wróblewski 《Veterinary parasitology》1976,2(3):303-306
Mebendazole was injected intraperitoneally at a dose of 40 mg/kg into six calves that had been inoculated 6 weeks earlier with eggs of T. saginata. The lethal effect of the drug on cysticerci was not significant in the mebendazole treated animals in comparison with those treated with a placebo. This was evaluated by counting the total number of cysticerci in each calf and the relative numbers of viable and degenerated cysts, an in vitro test for viability of cysticerci, and histological examination of the infected muscle tissue. 相似文献
3.
The distribution of Taenia saginata cysts was recorded in 23 experimentally infected calves. The calves harboured between two and 2569 cysts. A median of 15.7 per cent of the cysts were located in the heart and 6.5 per cent in the masseter muscles, the organs usually considered as the most important predilection sites. From these findings a simple probability model was used to estimate the meat inspection sensitivity at different infection levels. It was concluded that the possibility of detecting infected cattle is limited especially when, as in Denmark, the majority of animals are lightly infected. 相似文献
4.
Serological diagnosis of cysticercosis by an enzyme-linked immunosorbent assay in experimentally infected cattle. 总被引:1,自引:0,他引:1
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Taenia saginata infections were established in four groups of calves by administering doses of 10, 10(2), 10(3) and 10(4) infective eggs respectively by gavage. A fifth group remained as uninfected controls. Sera were collected from all calves over a period of 210 days. The sera were examined by an enzyme-linked immunosorbent assay (ELISA) with a fraction of larval Taenia hydatigena cyst fluid as antigen for the presence of anti-T. saginata IgG antibodies. At slaughter, the tongue, masseter, diaphragm and cardiac muscles and liver were examined for cysticerci. The higher dose rates of T. saginata eggs were reflected in higher numbers of cysticerci found in the calves at necropsy. There was also a correlation between higher levels of antibodies produced as measured by the ELISA and the numbers of eggs given. Sero-conversion was first detected about 25 days postinfection in heavy infections and later in the lighter infections. Maximal levels of antibody occurred between 40 and 60 days postinfection, followed by a gradual decrease in levels of antibody. A secondary increase in antibody occurred between 160 and 200 days postinfection which might have been due to release of antigen after death of the cysticerci. The low level of circulating antibodies in light infections may result in false positive or false negative diagnoses depending upon the selection of the cut-off point. 相似文献
5.
为了探讨细粒棘球蚴感染羊肝脏包囊纤维化形成的病理学过程,本研究通过病理组织学、细胞化学和超微形态学的诊断方法,对羊感染细粒棘球蚴后肝脏的病理组织学变化,以及肝组织纤维化和包囊形成过程进行病理学观察。结果显示:原头蚴感染肝组织后病变沿感染组织的血管周围产生,相继出现肝细胞萎缩、变性、坏死;同时病变组织血管壁出现纤维组织分解、血管管壁出芽,增生出的纤维组织伸向周围炎症区域,血管管腔及炎区大量嗜酸性粒细胞浸润,增生的胶原纤维沿残存的肝细胞周围围绕病变组织,最终形成包囊壁。该研究结果为进一步阐释细粒棘球蚴感染羊肝脏包囊纤维化形成的病理机制奠定了坚实的基础。 相似文献
6.
Boa ME Kassuku AA Willingham AL Keyyu JD Phiri IK Nansen P 《Veterinary parasitology》2002,106(2):155-164
The distribution and density of cysticerci of Taenia solium among distinct carcass sites was determined in 24 naturally infected finished pigs from Mbulu district, Tanzania. The heart, tongue, internal and external masseters, triceps brachii, lungs, liver, kidneys, psoas, diaphragm and brain of each pig as well as the muscles from the forelimb, hind limb, abdomen, head and thorax from one half of each pig carcass were all designated as distinct carcass sites and sliced in such a way that all fully developed cysts could be revealed and enumerated (i.e. each slice was less than 0.5 cm thick). The carcasses harboured from 76 to 80,340 cysts in total. Carcass sites which harboured the highest proportion of cysts were those of the hind and forelimbs (mean: 27.7 and 24.5%, respectively, of the total cysts in the carcass), while lower proportions were found in the tongue, heart, triceps brachii, and diaphragm (7, 3.6, 2 and 2, respectively). Relative cyst density was calculated for the different carcass sites by dividing the mean proportion of the total weight of the tissue groups into the mean proportion of cysts located in that site. The cysticerci in the examined distinct carcass sites were found in the following order of relative density: psoas muscles (10.5), internal masseter (8.1), external masseter (7.1), triceps brachii (4.9), forelimb (4.0), head muscles (3.8), tongue (3.4), hind limb (3.2), diaphragm (2.4), heart (1.9), abdominal muscles (1.3), trunk muscles (1.1), brain (1.0) and oesophagus (0.3). The proportion of cysts expected to be found at the surfaces exposed by visual examination or incision at meat inspection was calculated using an indirect method, which incorporated the area revealed by incision and visual inspection of an organ and the proportion of cysts located in the particular organ. It was estimated that 10.6% of the cysts would be located at inspected sites if regulations were followed carefully. 相似文献
7.
Tolosa T Tigre W Teka G Dorny P 《The Onderstepoort journal of veterinary research》2009,76(3):323-326
A cross-sectional study to determine the prevalence of bovine cysticercosis and hydatidosis was conducted from October 2007 to March 2008 in cattle slaughtered at the Jimma municipal abattoir. Cyst distribution and viability of bovine cysticercosis and hydatidosis were also determined. A total of 512 carcasses were inspected of which 15 (2.93%) and 161 (31.44%) were infected with Taenia saginata metacestodes and hydatid cysts, respectively. From a total of 109 cysticerci collected from infected carcasses, 47 (43.12%) were viable. The anatomical distribution of the cysticerci was, shoulder muscle (39.5%), heart (33.9%), neck muscle (13.8%), tongue (10.1%), masseter muscles (1.8%) and diaphragm (0.9%). Of the 1171 hydatid cysts collected 223 (19.0%) were fertile, 505 (43.1%) sterile, 49 (29.8%) calcified and 94 (8.0%) contained pus. A greater proportion of fertile cysts were found in the lungs than in other organs. It was concluded that these zoonotic cestodes deserve due attention to safeguard public health, and that further studies are needed on genotyping, epidemiology and public health importance of Echinococcus granulosus in the study area. 相似文献
8.
Calves were first infected with 5000 Taenia saginata eggs at six to 10 weeks old and treated with praziquantel 12 weeks later. Complete immunity against challenge lasted for at least 12 weeks following anthelmintic treatment. Six months after drug treatment over 90 per cent of the cysticerci had been completely absorbed but some were still detectable especially in the heart. An increase was observed in the ELISA values of sera from infected calves following treatment with praziquantel, but no such rise was detected in sera from resistant calves after challenge infection. 相似文献
9.
Kinetics of circulating antigens in pigs experimentally infected with Taenia solium eggs 总被引:3,自引:0,他引:3
Nguekam A Zoli AP Vondou L Pouedet SM Assana E Dorny P Brandt J Losson B Geerts S 《Veterinary parasitology》2003,111(4):323-332
Three groups of four piglets were experimentally infected with different doses (10(3), 10(4) and 10(5)) of Taenia solium eggs whereas a fourth group of two pigs received gravid proglottids. At autopsy 6 months post infection, the two latter pigs were heavily infected with more than 3000 living cysts per kg of muscle. Ten of the 12 other pigs harboured light infections, i.e. between 2 and 107 cysticerci, 42.4% of which were degenerated. The two remaining pigs had no detectable cysts at post mortem examination. Circulating antigens (CA) were detected in the sera of all pigs harbouring living cysticerci using a monoclonal antibody based ELISA. CA were first detected between 2 and 6 weeks post infection and remained present generally throughout the entire observation period even in pigs carrying only five to eight living cysts, although strong fluctuations of the level of CA were observed in some pigs. In animals without living cysts at post mortem CA were only detected for a short period and disappeared presumably when the cysticerci became degenerated. The minimum number of living cysts, which could be detected using this ELISA, was 1. 相似文献
10.
In two calves with massive cysticercosis and one healthy control calf the method of electrocardiographic examination was applied to a preciser determination of the clinical finding. The examination was performed on the 75th day after the experimental infection. Singly recorded electrocardiographic findings provided no characteristic symptoms indicating an injury of the conductive system of the heart. However, in both infected calves a lowering of the pulse frequency was found and in one of them also a flattening and a change of the polarity of the T wave. Autopsy and histological examination of the heart of the infected calves performed 111 days and 250 days after the infection revealed an attacking of the cardiac muscle by cysticerci and dispersed chronic myocarditis. This finding and the investigated dynamics of pathological changes in the course of cysticercosis in other experimental animals indicate that at the time of the electrocardiographic examination cysticercoid myocarditis at a stage of rest was recorded. 相似文献
11.
During the first 5 months of 1985, 35 moose were obtained for necropsy at the National Veterinary Institute, Uppsala, Sweden. 17 (49 %) of these cases were found to be infected with Elaphostrongylus sp. The nematodes were found in the epidural space of the spinal cord and brain, around and in the sciatic nerves and in muscle fasciae. Predilection sites appeared to be near cauda equina and the fasciae of the thoracic, gracilis and sartorius muscles.Most of the infected animals were calves. Both sexes were equally represented. Pathological changes found were characterized by focal haemorrhages and oedema. The changes were present in the muscle fasciae and in the leptomeninges of the brain and spinal cord. Beside infiltrates of mainly lymphocytes and plasma cells, adult nematodes, larvae and eggs were observed.Cases with mild gliosis in the cerebrum and degenerative changes of peripheral nerve roots affecting the epi- and perineurium were also found. In one case the inflammatory changes reached the endoneurium of a sciatic nerve bundle and in another case into a ganglion.Inflammatory reactions against the parasite were mainly directed towards eggs. In loose connective tissue close to nerve tissue in the central nervous system larvae were found close to eggs and remains of eggs indicating that eggs may hatch at this site.This investigation shows that natural infections with Elaphostrongylus sp. occur in wild moose and may produce pathological changes in large peripheral nerves and in the central nervous system. 相似文献
12.
猪囊虫病基因工程疫苗用于免疫治疗的研究 总被引:2,自引:0,他引:2
应用猪囊虫病基因工程疫苗分别对实验性猪囊虫病和自然感染的猪囊虫病进行了分组治疗,以探讨该疫苗的免疫治疗作用。10头人工复制的囊虫病猪,随机分成3组。第1组4头猪,于感染于1个月注射该疫苗,连续3次,间隔15天;第2组4头猪,于感染后2个月注射疫苗,同上连续3次;第3组2头猪,不注苗。第1组注射疫苗后1个月循环抗原滴度开始降低,2.0、2.5及3.5个月各有1头猪CA转阴,5个月OD值分别为0.14、0.09、0.17、0.38。第2组和第3组各猪血清OD值一直维持在1.0以上。感染后5个月剖检,第1组4头猪均未检出囊虫;第2组4头猪在咬肌、膈肌、腰肌、股内侧肌、肩胛外侧肌等部位均检测到囊虫,检测部位40cm^2面积发现囊虫3~7个,有部分虫体已钙化,胆汁卵化率为15.4%;第3组各猪也在各检测部位检测到囊虫,4 相似文献
13.
Attempted infection of calves with Taenia crocutae cysticerci and their subsequent serological response 总被引:1,自引:0,他引:1
The susceptibility of naive cattle to infection with cysticerci of Taenia crocutae was tested using three six- to nine-month-old Ayrshire bull calves, previously unexposed to infection with taeniid eggs. One calf was given 10,000 T crocutae eggs orally, another 5000 hatched unactivated oncospheres orally and the third 5000 hatched and activated oncospheres by intravenous injection. None of the calves contained viable cysticerci at post mortem examination 15 to 17 weeks later. All three calves contained small numbers of lesions in the liver and lesions were also present in the lungs of the calf which received oncospheres intravenously. All the calves developed an antibody response which was most pronounced in the calf given hatched unactivated oncospheres orally. 相似文献
14.
Kebede N 《Research in veterinary science》2008,85(3):522-526
The occurrence of cysticercosis due to Taenia saginata in cattle slaughtered for meat in Amhara National Regional State, northwestern Ethiopia between September 2005 and February 2007 was investigated. Routine meat inspection of various organs of 4456 cattle in eight abattoirs of this region showed that 824 (18.49%) were infected with Cysticercus bovis. The occurrence rate did not vary significantly from abattoir to abattoir (P>0.5). The tongue, masseter muscles, heart muscles, triceps muscles and thigh muscles were the main predilection sites of the cysts. Of 4102 male cattle, examined, 768 (18.72%) had cysts of C. bovis while 56 (15.82%) of the 354 female animals investigated were infected. The animals slaughtered were all adults. No significant difference in occurrence was recorded between the sexes. Monthly occurrence of the cysts in the animals revealed a rise of infected animals during the dry season. 相似文献
15.
B. M. McCraw 《Canadian journal of veterinary research》1973,37(1):21-24
Naturally occuring outbreaks of Ascaris suum infection in calves have usually beeen found in animals nine to 12 months of age. The circumstances surrounding these outbreaks suggest that yearling calves are either particularly susceptible to a primary exposure to A. suum or react strongly to A. summ after sensitization early in life to this or some related ascarid. To determine the effect of reinfection with A. suum nine to 12 months after varying levels of exposure to this nematode, six calves were inoculated with 200,000 to 9,000,000 eggs. Neither death nor, in general, severe clinical signs resulted from reinfection. All calves were examined 15 days after reinfection with pathological changes noted only in the lungs and consisting of emphysema, alveolar wall thickening as well as accumulations of fibrin, eosinophils and hemorrhage in the lumina of alveoli. The findings suggested that exposure to A. suum early in life is not a factor in the development of disease in calves infected at one year of age. It was also found that the eosinophilia that develops following a primary infection with A. suum evidently persists for at least one year. 相似文献
16.
Six Holstein-Friesian calves 3 to 5 months old, were used in a small-scale experiment to determine the pathogenic effects of the thread-necked strongyle Nematodirus helvetianus. Two calves were kept as controls and 4 were infected, 2 with single doses of 25,000, and 2 with 50,000 N helvetianus larvae. Observations made during the 8 weeks of the experiment found that N helvetianus is capable of producing severe pathologic changes in the intestine of previously healthy calves. At 38 days after infection, the calves infected with 25,000 and 50,000 larvae gained 32.9% and 72.4% less weight respectively, than their controls. An increase in total lymphocytes was observed with no significant change in hemoglobin concentration. Adult worms were found to penetrate the intestinal mucosa with considerable destruction, including tunneling. Rectal temperatures were elevated in infected animals. The prepatent period for N helvetianus in single infection was 18 to 19 days. A spontaneous expulsion of the adult parasites was observed in 1 of the calves exposed to 50,000 larvae. Worms recovered in this animal numbered 565, whereas the number of worms recovered in another calf infected with 50,000 larvae was 21,860. 相似文献
17.
F. Biancifiori C. Rondini V. Grelloni T. Frescura 《Comparative immunology, microbiology and infectious diseases》1986,9(4):337-346
Two groups of 13 new-laying hens each were infected by crop-route with 5000 and 50,000 infective oocysts of T. gondii. Four groups of 5 pigeons each were inoculated by crop-route with 50, 500, 1000 and 5000 infective oocysts. To each group of infected birds suitable controls were added. Hens from the experiment with 5000 infective oocysts were apparently resistant to the infection and they had no clinical signs in the succeeding 40 days p.i. Hens from the experiment with 50,000 infective oocysts showed an egg-drop and mortality in embryonated eggs, especially during the first 2 weeks p.i. Isolation of the parasite was unsuccessfully attempted from 720 embryonated eggs, produced by infected groups, and tested on various days p.i. and at different stages of infection. The parasite was isolated from the brain, heart, liver, spleen and lung of infected birds 7 and 15 days p.i.; 40 days p.i. it was evident only in brain and heart. IgG onset and mean course were monitored by ELISA and high titers were reached by both groups. Pigeons from groups 500, 1000 and 5000 developed rapidly progressive clinical signs as diarrhea, trembling, incoordination, torcicollis and death. They had enlargement of liver and spleen and focal necrosis, nodular features in the crop. Pigeons from expt 50 had no clinical signs in spite of the presence of the parasite in their organs for over 45 days p.i. Parasite was isolated from brain, heart, liver, spleen, lung, kidney, crop and muscles from all infected groups. Histopathological and ultrastructural features revealed the presence of multiplying tachizoites even within cells of the crop. Seroconversion, as monitored by ELISA, was recorded in all infected groups although high ELISA-titres were never reached. One of the negative controls from expt 5000 developed specific antibodies but the parasite was not isolated from its organs. 相似文献
18.
The prevalence of Taenia saginata cysticercosis in cattle slaughtered for meat in Addis Ababa Abattoir, Ethiopia between September 2004 and August 2005 was
reported. The examination of various organs of 11227 cattle in Addis Ababa Abattoir showed that 842 (7.5%) were infected with
T. saginata cysticercosis. The tongue, masseter muscles, cardiac muscles, triceps muscles and thigh muscles were the main predilection
sites of the cysts. The cysts of bovine cysticercosis were also identified on the spleen, intercostal muscles, diaphragm and
liver. Out of 10329 male cattle, examined, 783 (7.6%) had cysts of bovine cysticercosis while 59 (6.6%) of the 898 female
animals investigated were infected. The animals slaughtered were all adults. No significant difference in prevalence rates
was recorded between the sexes. The prevalence of bovine cysticercosis was higher in local zebu cattle breeds than Holstein-Frisian
cattle. 相似文献
19.
de Aluja AS Villalobos AN Plancarte A Rodarte LF Hernandez M Zamora C Sciutto E 《Veterinary parasitology》1999,81(2):129-135
The present study demonstrates that pigs experimentally infected with Taenia solium eggs develop resistance to reinfection that lasts at least five months. Thirteen 2-month-old piglets were infected with eggs of Taenia solium. After 5 months, two pigs were euthanized and five were challenged with eggs from a second tapeworm. Nine months after the first infection, six pigs were challenged with a third tapeworm. All 11 challenged pigs were euthanized 2 months after reinfection. In order to confirm the infectivity of the eggs, several piglets were inoculated with each taenia. Two of the five pigs reinfected after 5 months did not develop metacestodes, two showed few caseous non-infective forms and in the fifth pig, 14% of the metacestodes were vesicular and 86% colloidal and caseous. In the six animals challenged 9 months after the first infection, three were heavily infected with vesicular metacestodes and the other three showed only colloid and caseous forms in muscles. All parasites found in brains were vesicular. We conclude that immunity due to primary infection lasts at least 5 months. At 2 months of infection antigens of 24 and 39-42 kDa were the most frequently recognised. In those pigs with only a few caseous cysts in muscles and/or vesicular ones in brains no antibodies were detected. 相似文献
20.
The distribution of 12,310 cysts of Taenia ovis in 212 experimentally infected lambs and of 46 cysts in 24 naturally infected 5-year-old sheep, has been analysed. It was determined that irrespective of the number of cysticerci in an infected group of animals, about two-thirds were present in the carcase meat, and the remainder were distributed between the heart, diaphragm and head including the tongue. In lightly infected animals, cysticerci were not necessarily present in any of these sites. In individual animals, the number of cysticerci present in any one or in all three of these sites, did not provide accurate information on the extent of the infection in the carcase meat. 相似文献