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用微生物法测定莫能菌素并筛选出合适的工作菌,分别选用短小芽孢杆菌和枯草芽孢杆菌作为工作菌摸索条件、制备标准曲线、测定最低检测限。结果表明,采用枯草芽孢杆菌的最低检测限为1μg/ml,较短小芽孢杆菌的1.25μg/ml要低;枯草芽孢杆菌标准曲线的相关系数为0.9983,较短小芽孢杆菌的0.994要高,说明实验有良好的线形关系;五组工作曲线的变异系数分别为5.1%和1.7%,说明本方法重现性较好。因此,应提倡采用枯草芽孢来检测莫能菌素。 相似文献
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UPLC-MS/MS法同时测定鸡肉中的莫能菌素和盐霉素残留 总被引:1,自引:0,他引:1
建立了同时测定鸡肉中莫能菌素和盐霉素残留检测方法。在国内外已有研究的基础上,对样品前处理和仪器条件进行了改进,采用乙腈提取鸡肉中莫能菌素和盐霉素残留,石墨化碳黑SPE小柱对提取液进行净化,然后用Waters ACQUITY UPLCTMTQD超高效液相色谱-串联质谱仪测定。结果表明,莫能菌素和盐霉素的检测限为0.2μg/kg,最低定量限为0.5μg/kg。选取0.5、1.0、2.0μg/kg三个浓度进行空白添加回收率实验,回收率为71.9%~95.1%,批内变异系数和批间变异系数均小于15%,适合大量鸡肉样品中莫能菌素和盐霉素的残留检测。 相似文献
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饲料中恩拉霉素的微生物学含量测定方法研究 总被引:2,自引:0,他引:2
研究并建立了微生物学法测定饲料中恩拉霉素含量的方法.饲料样品经酸性甲醇溶液提取,大孔吸附树脂层析柱吸附洗脱后,饲料中的恩拉霉素得到很好的分离纯化.本方法采用枯草芽孢杆菌为检验菌,饲料中恩拉霉素的最低定量限为0.5 mg/kg,标准曲线的线性范围为0.4~12.8 u/mL,相关系数R为0.999 1,回收率大于85%,RSD小于15%.该方法适用于饲料中恩拉霉素的检测. 相似文献
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建立一种测定牛肌肉组织中加米霉素残留的高效液相色谱-串联质谱方法。均质后的样品经0.1 mol/L磷酸二氢钾溶液提取,正己烷脱脂,HLB固相萃取柱净化,反相C 18色谱柱分离,采用多反应监测正离子模式测定。结果显示,该方法检测限为0.5μg/kg,定量限为2μg/kg;在2-400μg/kg范围内线性良好,相关系数r均大于0.999;平均回收率范围为91.6%-118.7%,批内变异系数在2.06%-11.06%之间(n=6),批间变异系数在3.86%-7.98%之间(n=18)。该方法灵敏、准确、重复性好,适用于牛肌肉组织中加米霉素残留量的测定。 相似文献
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高效液相色谱法检测鱼肉中甲砜霉素残留的研究 总被引:7,自引:0,他引:7
用高效液相色谱法检测鱼肉中甲砜霉素残留。采用乙酸乙脂超声波提取鱼肉中残留的甲砜霉素,紫外检测波长225nm,流动相为甲醇:水[60:40(V/V)],流速1.2ml/min,进样量10μl,用色谱柱分离后在高效液相色谱仪上进行检测。在以上色谱条件下,内标物氟苯尼考的均保留时间为1.970min;甲砜霉素的均保留时间为2.239min,在鱼肉中的最低检测限为0.05μg/g,标准曲线的线性范围为0.02~16μg/ml,线性相关系数为0.9998。甲砜霉素浓度为0.05、0.5、40μg/g时鱼肉回收药物的均回收率为92.08%~105.38%;批间变异系数分别为3.61%、2.33%、3.56%。结果表明,高效液相色谱法是一种快速、灵敏、可靠的检测鱼肉中甲砜霉素残留的分析方法。 相似文献
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超高效液相色谱-串联质谱法测定水产品中群勃龙残留方法研究 总被引:1,自引:0,他引:1
建立了牛奶中苯并咪唑类药物及代谢物残留的超高效液相色谱-串联质谱检测方法。水产样品经乙酸乙酯提取,C18固相萃取柱净化,采用超高效液相色谱-串联质谱多反应监测模式检测,外标法定量。不同基质中,群勃龙在2-100 ng/mL的浓度范围内呈现良好的线性相关,相关系数为0.994以上;本方法对群勃龙群勃龙在鱼、虾、鳖、蟹类产品中的检测限为0.5μg/kg,定量限为1μg/kg;在贝类产品中的检测限为1μg/kg,定量限2μg/kg;不同水产品的基质中添加1-10μg/kg浓度范围内,其回收率为74.2%-119%,批内变异系数在1.0%-9.0%之间,批间变异系数在0.3%-8.5%之间。本方法分析速度快,灵敏度高,重现性好,各项技术指标均满足国内外相关法规要求,可用于水产品中群勃龙的残留检测。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献