咪唑苯脲治疗犬巴贝西虫病

经皮下注射国产咪唑苯脲（６ｍｇ／ｋｇ体重，间隔２４ｈ用药 ２次）治疗巴贝西虫病病犬３２例，治疗后７ｄ以血液涂片复查３２例，２９例虫体消失，３例仍有虫体，治愈率为９０．６３％。 我们于１９９８年５月－９月期间，发现高热病犬３２条，经临床检查、血、尿常规化验及血液涂片的虫体检查，将症状明显、体表有硬蜱寄生、血片镜检发现有巴贝西虫体的３２条病犬用药物治疗，结果如下：１药物和给药方法咪唑苯脲二盐酸盐为白色或略带黄色粉剂，临用前用蒸馏水配成１０％溶液，按６ｍｇ／ｋｇ体重剂量皮下注射，２４ｈ后重复用药１次。２…     

一例孔雀封闭毛细线虫病的诊治

在一例表现为腹泻、消瘦死亡的孔雀病例中，从小肠内检出大量丝状的白色虫体。根据虫体和虫卵形态特征，确定为封闭毛细线虫，全群孔雀采用盐酸左旋咪唑治疗，2 d后疫情得到有效控制。     

角膜穿刺治疗马匹混睛虫

角膜穿刺治疗马混睛虫在兽医临床上虽然多用,但很难做到一次成功取出虫体。笔者近年接治了22匹病马,1次穿刺取出虫体20例,成功率为90%。方法如下: 1.针具选用针体粗细适中(相当于1.5寸铁钉)、针尖锋利的三棱针。穿刺后的针眼呈三角形,便于房水向外喷射,将虫体带出,术后角膜针孔对位     

伊维菌素浇泼剂对猪疥螨病的治疗

本实验选取2022年1～4月期间某猪场猪疥螨病患猪82头,分为研究组与对照组,研究组41头,采用伊维菌素浇泼剂治疗;对照组41头,采用虫克星治疗。结果研究组病猪治疗6d后虫体转阴率为60.98%,治疗12d后虫体转阴率为78.05%,治疗18d后虫体转阴率为95.12%,治疗24d后虫体转阴率为100.00%,均高于对照组（P<0.05）。可见伊维菌素浇泼剂对猪疥螨病疗效显著,是优质广谱驱虫药物,可推广应用。     

用1%敌百虫液注入法治疗马浑睛虫病

马浑晴虫病在临床上很少见,不仔细检查很难发现虫体在马眼前房液内游动。笔者在30多年的临床上仅见5例。发现后不及时治疗,由于虫体在眼内产生毒素以及游动的机械性刺激,病眼很快失明。降低了疫畜的利用价值和经济价值。马浑晴虫病的病原体有三种:牛指状丝虫、鹿丝状线虫、马丝状线虫的幼虫,其中间宿主为蚊类。一、临床症状由于虫体分泌毒素以及虫体在眼房液     

绵羊脑包虫的简易手术疗法

绵羊脑包虫(多头蚴)病是一种死亡率很高的寄生虫病,为了探索治疗方法,我乡畜牧兽医工作站的同志于1982—1983年先后开颅治疗了21例病羊,全愈16例,占76%,死亡2例,没有找到虫体3例,其中     

有关黄牛多头蚴病诊疗技术问题的探讨

黄牛多头蚴病,又称脑包虫病,现阶段比较常用的治疗方法仍然是采取手术将多头蚴自脑内摘除。但由于虫体在脑部寄生部位不易确定,虫体大小不易判定,虫体不易取出等诸因素的影响,疗效往往不够满意。     

虫必清治疗牛皮蝇蛆病

１９９５年４月以来我们共收治患牛皮蝇蛆的奶牛３４例。应用虫必清治疗，均获得满意效果。报告如下。１　虫体特征　寄生于牛背部皮下的牛皮蝇和纹皮蝇的成熟幼虫（第３期幼虫）。虫体长达２７～２８ｍｍ，直径８～１２ｍｍ，虫体分１１个节，后端有两个气门板，呈粗圆柱型，前后钝圆身体屈向背面，背面较平，腹面稍隆起。虫体颜色不一，分棕褐色、白色、灰褐色３种，剖开虫体全是混浊的白色液体。２　治疗方法２．１　药品来源　虫必清：东北农业大学科发兽药厂生产（黑饲添试字（９５）０４９００８），出厂日期１９９５年３月２８日。虫…     

鸭对体吸虫病的诊治

通过流行病学调查、临床症状和剖检病变观察、实验室检查及虫体鉴定结果,确诊了一例放养鸭对体吸虫病,采取有效治疗措施后病情得以控制。     

黄牛肝片吸虫病的诊治

黄牛肝片吸虫病是严重危害反刍兽的蠕虫病,又叫肝蛭病.虫体片形呈棕红色,一般长2 cm～3 cm,宽1 cm～1.3 cm,虫体寄生在牛的肝脏、胆管里,能引起胆管炎、肝炎、肝硬变.病牛营养下降,产奶量减少,甚至引起死亡,造成巨大损失,给养牛业带来严重危害.该病每年夏秋季节在我市时有发生,笔者通过多年兽医临床实践共治疗36例,治愈34例,治愈率94.4%,现将诊治方法介绍如下:     

Doxycycline clearance of experimentally induced chronic Ehrlichia canis infection in dogs

BACKGROUND: Ineffective clearance of Ehrlichia canis after doxycycline administration has been reported despite the fact that the recommended treatment for canine ehrlichiosis is doxycycline. The effectiveness of doxycycline in clearing E canis infection from the blood and tissues of dogs requires additional evaluation. HYPOTHESIS: Doxycycline (5 mg/kg PO q12h), administered for 4 weeks, will eliminate E canis infection from the blood and tissues of experimentally infected dogs. ANIMALS: Fifteen Walker hound-mixed breed dogs were inoculated subcutaneously with E canis-infected canine histiocytic cells 4 months before doxycycline treatment. METHODS: Four dogs were treated with doxycycline (5 mg/kg PO q12h for 3 weeks), 5 dogs were treated with doxycycline at the same dosage for 4 weeks, and 5 control dogs were not treated. Dexamethasone (0.4 mg/kg i.v.) was given after treatment to precipitate recrudescence of any remaining E canis organisms. Platelet counts, anti-E canis immunofluorescent antibodies, and polymerase chain reaction (PCR) detection of E canis deoxyribonucleic acid (DNA) in blood and tissues were evaluated. RESULTS: E canis DNA was not detected in the blood and tissues of doxycycline-treated dogs after treatment. Platelet counts were within reference intervals, and E canis antibodies decreased. Spontaneous clearance of E canis infection occurred in 2 of 5 control dogs. Three control dogs had E canis DNA detected in blood and tissues, platelet counts remained low or within the reference interval, and E canis antibodies remained high. CONCLUSIONS AND CLINICAL IMPORTANCE: As administered in this study, doxycycline cleared E canis from the blood and tissues of experimentally infected dogs.     

硝唑尼特治疗犬贾第虫病的研究

选择试验犬8只,将其随机分为4个小组,每组2只.连续6 d对8只试验犬分剐进行粪检,确定无贾第虫感染后,用体外纯培养的犬贾第虫滋养体接种试验犬,然后每天采集试验犬新鲜粪便40 g,用硫酸锌漂浮法进行粪检,当检测犬贾第虫感染呈阳性时,分别用1、2、4 mg/kg体质量剂量的硝唑尼特时1、2、3组试验犬进行灌服治疗,第4组试验犬不用药作为对照.用药后,每天以同样的方法检测贾第虫包囊,并计数.结果表明,以2、4 mg/kg体质量给药的试验犬1 d后粪检结果转为阴性,以1 mg/kg体质量给药的试验犬4 d后粪检结果转为阴性.结果表明以2、4 mg/kg体质量剂量的硝唑尼特对犬贾第虫痛有很好的疗效.     

Serosurvey of Ehrlichia canis and Hepatozoon canis infection in dogs in Yamaguchi Prefecture, Japan

Antibodies to Ehrlichia canis and Hepatozoon canis in dogs at the Animal Hospital in Yamaguchi University were surveyed and potential risk factors for both pathogens were evaluated. Among 430 dogs examined, 20 (4.7%) and 18 (4.2%) dogs showed positive findings for E. canis and H. canis, respectively. Neither, sex nor age was associated with the seropositivity of either pathogen, but the positive rate in dogs kept outside was slightly higher than that in dogs kept inside for both pathogens. A higher seropositive reaction to E. canis and H. canis was observed in dogs that lived in certain cities and towns. Beagles, golden retrievers and pointers had higher seropositivity than other breeds in E. canis, whereas shibas, akitas, beagles, pointers and mongrels had higher positive rates than other breeds in H. canis.     

The prevention of transmission of Babesia canis canis by Dermacentor reticulatus ticks to dogs using a novel combination of fipronil, amitraz and (S)-methoprene

Four groups of seven dogs were treated topically with a novel combination of fipronil, amitraz and (S)-methoprene in a spot-on formulation (CERTIFECT?, Merial Limited, GA, USA) on 28, 21, 14 and 7 days prior to tick infestation, respectively and acaricidal efficacy and transmission blocking compared with an untreated control group (seven dogs). All dogs were infested with adult Dermacentor reticulatus ticks harbouring Babesia canis canis. Babesia canis canis was transmitted by D. reticulatus to all seven untreated control dogs, confirmed following demonstration of clinical signs, by the detection of B. canis parasites in thin blood smears and B. canis canis PCR-RLB DNA assay on blood and the development of B. canis canis antibody titres by 14-21 days after tick infestation. The majority of treated dogs remained sero-negative for 42 days after infestation. Therefore, the treatment of dogs with CERTIFECT applied up to 28 days prior to infestation with D. reticulatus harbouring B. canis canis, successfully prevented the development of clinical signs of canine babesiosis.     

Failure of imidocarb dipropionate to clear experimentally induced Ehrlichia canis infection in dogs

The recommended treatment for canine ehrlichiosis is tetracycline or its analog doxycycline, although recent reports have documented ineffective clearing of Erchlichia canis after doxycycline administration. Imidocarb dipropionate is used as an alternative treatment to tetracycline or is used in conjunction with doxycycline. The effectiveness of imidocarb dipropionate in clearing Ehrlichia species from the blood and tissues of dogs with E. canis infection has not been thoroughly evaluated. Fifteen dogs were experimentally infected with E. canis. Ten dogs were treated with imidocarb dipropionate (6.6 mg/kg, IM, 2 injections given 2 weeks apart). Five infected control dogs were not treated. Blood samples from all 15 dogs were E. canis DNA positive by PCR assay by 3 weeks after inoculation (PI), and E. canis antibodies were detected by IFA assay by 1 week PI. Blood platelet counts in all dogs were below the reference interval by 4 weeks PI. E. canis DNA was detected in bone marrow and splenic aspirates by PCR assay 4 weeks PI but not before infection. Bone marrow aspirates were E. canis DNA positive by PCR assay in 14/15 dogs, and splenic aspirates were E. canis DNA positive by PCR assay in 13/15 dogs. Blood samples from all treated and control dogs remained positive for E. canis DNA by PCR assay, and platelet counts remained below preinoculation values 13 weeks PI (6 weeks after 2nd treatment). As administered in this study, imidocarb dipropionate did not clear experimental E. canis infection in dogs.     

Anti-Hepatozoon canis serum antibodies and gamonts in naturally-occurring canine monocytic ehrlichiosis

The prevalence of IgG antibodies to Hepatozoon canis and the presence of gamonts in the blood and hemolymphatic tissues were studied in dogs with canine monocytic ehrlichiosis (CME) caused by Ehrlichia canis. Both pathogens are transmitted by the tick Rhipicephalus sanguineus. Forty-five out of 69 (65.2%) dogs with CME were seropositive to H. canis by an enzyme-linked immunosorbent assay (ELISA). Intra-neutrophilic gamonts of H. canis were found in 2 out of 69 dogs (2.9%) comprising 4.5% of the seropositive dogs. The present study indicated that the prevalence of antibodies to H. canis was high among dogs with CME in an area where both infections are endemic. However, previous exposure to H. canis was not found as an important contributor to clinical or clinicopathologic abnormalities found in dogs with CME.     

Detection of Ehrlichia canis by PCR in different tissues obtained during necropsy from dogs surveyed for naturally occurring canine monocytic ehrlichiosis

A molecular study for the detection of Ehrlichia canis was carried out on tissues obtained at necropsy from randomly selected dogs with the intention of investigating naturally-occurring canine ehrlichiosis. The tissues evaluated for the presence of E. canis included lymph nodes, spleen, liver, bone marrow, and blood. Eight of the 18 dogs included were found to be positive for E. canis by polymerase chain reaction (PCR) and sequencing of the 16S rRNA gene. Two dogs were positive for Anaplasma platys of which one dog was co-infected with E. canis and A. platys. Blood (5/8) and lymph nodes (5/8) were the tissues found to yield the highest number of positive E. canis PCR results with 7/8 dogs positive in the blood or lymph node. E. canis and A. platys DNA could be amplified by PCR when tissue samples were obtained 72h after the time of death.     

A survey for infection with Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi, and Babesia canis in feral and client-owned dogs in the Turks and Caicos Islands, British West Indies

The frequency of infection with Dirofilaria immitis and Babesia canis and seropositivity to Ehrlichia canis and Borrelia burgdorferi in feral and client-owned dogs was determined. Feral dogs were 14.8 and 11.2 times more likely to be seropositive to D. immitis and E. canis, respectively, than were client-owned dogs. None of the dogs tested positive for B. burgdorferi or B. canis.     

Prevalence of Ehrlichia canis infection in thrombocytopenic dogs from Rio de Janeiro, Brazil

BACKGROUND: Infection with Ehrlichia canis causes a highly variable, multisystemic disease in dogs. Nevertheless, many clinicians in Rio de Janeiro, Brazil, use the presence of only thrombocytopenia to make a presumptive diagnosis of E canis infection. OBJECTIVE: The objective of this study was to determine the prevalence of E canis in thrombocytopenic dogs from Rio de Janeiro, Brazil, using polymerase chain reaction (PCR). METHODS: Following DNA extraction of whole blood samples from 226 dogs, PCR assays were done using primers for rickettsial DNA (including Ehrlichia spp, Anaplasma platys and A phagocytophilum) and using E canis-specific primers (16S rRNA gene). Dogs were grouped as thrombocytopenic and nonthrombocytopenic based on platelet counts. The null hypothesis that there was no difference in the prevalence of E canis in these groups was rejected at P<.05. RESULTS: Thirty-six (32.1%) of the thrombocytopenic dogs and 4 (3.5%) of the nonthrombocytopenic dogs were positive for rickettsial gene sequences (P<.0001). Further, 30 (26.8%) of thrombocytopenic dogs and 4 (3.5%) nonthrombocytopenic dogs were positive for E canis-specific gene sequences (P<.0001). CONCLUSIONS: Although the prevalence of E canis infection was higher in thrombocytopenic dogs, less than one third of these dogs had demonstrable E canis infection. Thus, thrombocytopenia is not specific for the detection of E canis infection and should not be used solely to establish a diagnosis of canine ehrlichiosis, even in a geographic area with relatively high disease prevalence.     

Concurrent babesiosis and ehrlichiosis in the dog: blood smear examination supplemented by the indirect fluorescent antibody test, using Cowdria ruminantium as antigen

Giemsa-stained, peripheral blood smears of 67 dogs, showing clinical signs typical of babesiosis or reminiscent of concurrent babesiosis and ehrlichiosis, were examined for the presence of Babesia canis and Ehrlichia canis. Since Cowdria ruminantium cross-reacts with Ehrlichia, the sera of these dogs were also subjected to the indirect fluorescent antibody (IFA) test in which C. ruminantium was used as antigen. Fifty-five per cent of these dogs had mixed infections of B. canis and E. canis, as judged by blood smear examination and serology. The serum of 32% of these dogs with mixed infections reacted positively in the IFA test. Six out of 9 dogs, the blood smears of which were negative for both B. canis and E. canis, were serologically positive for E. canis. Furthermore, sero-conversion from a negative in the initial serum sample to titres of up to 1:160 in a subsequent sample was recorded in 9 out of 13 dogs with suspected mixed infection on blood smear.