PM 7/4(2): Bursaphelenchus xylophilus

Specific scope

This standard describes a diagnostic protocol for Bursaphelenchus xylophilus ¹ 1 Use of brand names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable.
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Specific approval and amendment

First approved in 2000–09. Revised on 2009–09.

     

PM 9/1(2): Bursaphelenchus xylophilus and its vectors: procedures for official control

Specific scope

This standard describes the procedures for official control with the aim of containing and eradicating Bursaphelenchus xylophilus in areas where infestation of susceptible coniferous species by B. xylophilus leads to wilt symptoms.

Specific approval and amendment

First approved in 2002–09. Revision approved in 2009‐09.

     

PM 7/24 (3) Xylella fastidiosa

Specific scope

This Standard describes a diagnostic protocol for Xylella fastidiosa. 1 It should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols.

Specific approval and amendment

First approved in 2004‐09. Revised in 2016‐09 and 2018‐04. 2     

PM 7/29 (3) Tilletia indica

Specific scope

This Standard describes a diagnostic protocol for Tilletia indica. 1 It should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols.

Specific approval and amendment

This Standard was originally developed under the EU DIAGPRO Project (SMT 4‐CT98‐2252) by a partnership of contractor laboratories and interlaboratory comparison in European countries. First approved as an EPPO Standard in 2003–09. First revision approved in 2007–09. Second revision approved on 2017–11. Although this EPPO Diagnostic Standard differs in terms of format it is in general consistent with the content of the IPPC Standard adopted in 2014 on Tilletia indica (Annex 4 to 2006 ) with the following exceptions. (1) In the EPPO region, as the pest is not present, a higher confidence in the results is required, a sieve wash test should be carried out (optional in the IPPC protocol). (2) When fewer than 10 teliospores are found the options should allow testing the (<10) teliospores with conventional or real‐time PCR (this was not an option in the IPPC protocol flow chart, although it was stated that direct real‐time PCR could be used on individual teliospores in the text). (3) The method for extracting teliospores from untreated seed or grain by size‐selective sieving is slightly different based on the experience in the region (European Union test performance study). The EPPO Diagnostic Standard also includes a test for a direct real‐time PCR for use on pellets (developed in 2016). Some additional information on methods for morphological identification, from the former version of the EPPO Standard, which are not in the IPPC protocol are included in this protocol in Appendix 3 as they were considered useful by the members of the Panel on Diagnostics in Mycology.     

浅谈松材线虫病监测工作中的几个问题

本文结合当前开展松材线虫病的监测工作,对其中几个需解决的实际问题,提出相应对策。     

PM 7/40 (4) Globodera rostochiensis and Globodera pallida

Specific scope

This Standard describes a diagnostic protocol for Globodera rostochiensis and Globodera pallida. 1 Terms used are those in the EPPO Pictorial Glossary of Morphological Terms in Nematology. 2 This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols.

Specific approval and amendment

Approved as an EPPO Standard in 2003‐09. Revisions approved in 2009‐09, 2012‐09 and 2017‐02.     

PM 8/7 (1) Populus

Specific scope

The purpose of the EPPO Standard on Populus is to recommend to EPPO Member Governments the phytosanitary measures which they should use or require for Populus plants and plant products moving in international trade to prevent the introduction and spread of quarantine pests. Some of these recommendations are addressed to all EPPO Member Governments, others are addressed only to countries considered to face a certain level of risk from the introduction and spread of the regulated pests concerned. All these recommendations were derived from:

• EPPO Standards PM 1/2 (EPPO A1 and A2 Lists) and the former EPPO Standards PM 2 (pest‐specific phytosanitary measures), or
• Pest Risk Analysis (PRA) carried out or reviewed by EPPO, or
• the Working Party on Phytosanitary Regulations.

For wood packaging material, recommendations are based on ISPM 15 Regulation of wood packaging material in international trade.

Specific approval and amendment

First approved in 2017‐09.