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1.
Linseed as a feed ingredient contains a number of constituents, such as mucilage, gel forming polysaccharides, structural carbohydrates and ω-3 fatty acids, which could have functional properties in relation to maintaining and supporting gastro-intestinal health in post weaning piglets. Intestinal gene expression was studied in weanling piglets fed diets containing either or not linseed or linseed expeller meal. Piglets were either or not orally challenged with an enterotoxigenic strain of E. coli K88. Pairwise comparisons identified several mRNAs expressed significantly different, in particular between treatments C− (control, not challenged) and LS− (linseed, not challenged) and LS+ (linseed, challenged) and LSM+ (linseed expeller meal, challenged). The intestinal micro-array technique is a promising tool to study the relationship between nutrition and feeding and gut tissue responses under defined conditions. 相似文献
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试验旨在探索对仔猪床进行加温处理以及采用定时饲喂的方式的对早期断奶仔猪不同肠段黏膜形态和肠道发育的影响,从而为确定断奶仔猪适宜的饲养温度和科学的饲喂方式提供理论依据。试验采用2×2因子设计。选用21日龄杜长大断奶仔猪240头,随机分到4个处理组:1):28~30℃的仔猪床加温处理组;2)23~25℃的低温对照组(炉火暖气管道舍内加热);加温处理组和低温对照组中又根据不同的饲喂模式分为每天6次定时饲喂组和自由采食组。每个处理6个重复,每个重复10头猪。经过14 d的试验之后,在每圈仔猪中随机抽取1头,屠宰后取样检测小肠黏膜的绒毛高度、隐窝深度和肠壁厚度。结果表明:加温饲养组(28~30℃)的仔猪空肠中段的绒毛高度显著高于低温对照组(23~25℃)(P<0.05)。加温饲养组十二指肠的隐窝深度和空肠中段的肠壁厚度显著低于低温对照组(P<0.05)。定时饲喂饲养的仔猪空肠后段的绒毛高度显著高于自由采食饲养的仔猪(P<0.05)。定时饲喂组仔猪空肠中段的肠壁厚度和十二指肠隐窝深度显著低于自由采食组(P<0.05)。2因子的互作效应表明:加温定时饲喂组饲养的仔猪空肠后段的绒毛高度显著高于其他处理组。低温自由采食组饲养的仔猪空肠后段的绒毛高度极显著低于其他处理组(P<0.01)。加温定时饲喂组的仔猪空肠中段的肠壁厚度极显著低于其他处理组(P<0.01)。由结果可知,28~30℃的加温饲养组以及定时饲喂的饲喂模式有利于仔猪的肠道黏膜形态的改善,有益于肠道的发育和健康。 相似文献
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为了探明大豆酶解蛋白替代仔猪饲粮中血浆蛋白粉、肠膜蛋白或鱼粉的功效。研究选择28 d断奶三元杂交(杜×长×大)仔猪320头,随机分为4个处理,每个处理4个重复,每个重复20头。分别饲喂:处理1组:3%血浆蛋白+3%肠膜蛋白+3%鱼粉日粮;处理2组(无血浆蛋白质):9%大豆酶解蛋白+3%肠膜蛋白+3%鱼粉日粮;处理3组(无肠膜蛋白):4%大豆酶解蛋白+3%血浆蛋白+3%鱼粉日粮;处理4(无鱼粉):3.5%大豆酶解蛋白+3%血浆蛋白+3%肠膜蛋白日粮。试验期28 d,于试验14 d直肠取粪便测定微生物,28 d采集血液测定血清抗体水平。研究结果证明:与常规的血浆-肠膜-鱼粉日粮相比,9%大豆酶解蛋白+3%肠膜+3%鱼粉的无血浆蛋白粉日粮,可以获得完全相同的增重和腹泻率(P0.05),但是,采食量和料重比显著降低(P0.05);4%大豆酶解蛋白+3%血浆+3%鱼粉的无肠膜蛋白日粮处理提高了日增重,降低了料重比和腹泻率(P0.05);3.5%大豆酶解蛋白+3%血浆+3%肠膜的无鱼粉日粮处理获得了最高的试验期末体重、日增重和采食量,显著降低了料重比和腹泻率(P0.05)。仔猪成活率处理之间均没有显著影响(P0.05)。4%大豆酶解蛋白+3%血浆+3%鱼粉的日粮组获得了最低的大肠杆菌总数;9%大豆酶解蛋白+3%肠膜+3%鱼粉组获得了最高的乳酸菌总数。无大豆酶解蛋白日粮组的大肠杆菌总数最多,乳酸菌总数最少。9%大豆酶解蛋白+3%肠膜+3%鱼粉组获得了最高的IgA和IgM抗体水平(P0.05)。由此证明,使用9%的大豆酶解蛋白可以替代日粮中3%的血浆蛋白粉,4%的大豆酶解蛋白可以替代日粮3%的肠膜蛋白质,3.5%大豆酶解蛋白可以替代日粮中3%的鱼粉。大豆酶解蛋白替代血浆蛋白粉可以改善断奶仔猪的生长性能,调节肠道菌群,增加免疫力。 相似文献
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Nucleotides and carob pulp were tested as possible alternatives to antimicrobial growth promoters for newly weaned pigs. Four dietary treatments were used: basal diet, nucleotides, carob pulp and nucleotides + carob pulp. Performance was measured in a group of 72 pigs. Another group of 60 pigs was used for histological measurements of the jejunal mucosa and microbiological measurements by RFLP in ileum and caecum at different post-weaning intervals. Treatment did not affect performance. On the other hand, the morphology parameters of the jejunum were significantly (P < 0.001) affected by day after-weaning. No effects (P > 0.05) of treatment were observed on these parameters. The similarity (intra and inter-group) of the intestinal microbiota decreased with day after-weaning (P < 0.001) and was also affected by dietary treatment (P < 0.05). The nucleotide diets had the highest similarities in the ileum whereas the carob pulp diets had the lowest in the caecum. It is concluded that both nucleotides and carob pulp are able to modulate the changes in microbiota composition after weaning and that whereas nucleotides act in the ileum, carob pulp acts in the caecum. 相似文献
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肠道黏膜免疫系统是仔猪体内最大的黏膜免疫器官,在识别和清除有害物质的过程中发挥着重要作用,同时能够对营养物质和肠道益生菌形成免疫耐受,与仔猪的健康关系最为密切。随着养殖业进入全面减抗、限抗的时代,养猪业面临巨大的挑战。仔猪肠道健康决定了猪场的生产效益,正确认识仔猪肠道免疫系统,科学调节肠道免疫,提高仔猪抗病能力,是决定养猪生产能否成功的重要因素。 相似文献
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在断乳仔猪日粮中添加脂肪酸优化的脂肪源,同时以椰子油粉作为对照,研究优化的脂肪源对断乳仔猪生长性能、腹泻情况及生产效益的影响。试验选取120头断乳仔猪,随机分为2个处理组:A组添加4%结构脂脂肪粉(商品名:乳脂能Ⅱ型),B组添加4%椰子油粉(商品名:椰香50),每组6个重复,每个重复10头猪,试验为期12 d。结果表明:A组仔猪的采食量、日增重及成活率均高于B组,且A组仔猪的腹泻率显著低于B组。经济效益分析表明:试验期间,A组每头猪增重回报比B组增加15.52元/头,净盈利增加13.54元/头。由此可见,脂肪酸优化的结构脂可促进仔猪采食,提高仔猪增重,减少仔猪腹泻,且其经济效益优于B组。 相似文献
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《动物营养(英文)》2020,6(2):152-159
This experiment was conducted to investigate the effects of benzoic acid, Bacillus coagulans and oregano oil combined supplementation on growth performance, immune status and intestinal barrier integrity of piglets. In a 26-d experiment, 25 piglets were randomly assigned to 5 treatments: 1) a basal diet, negative control (NC), 2) NC added with antibiotics, positive control (PC); 3) NC added with benzoic acid at 3,000 g/t and Bacillus coagulans at 400 g/t (AB); 4) NC added with benzoic acid at 3,000 g/t and oregano oil at 400 g/t (AO); 5) NC added with 3,000 g/t benzoic acid and Bacillus coagulans at 400 g/t and oregano oil at 400 g/t (ABO). On d 27, all piglets were euthanized to obtain jejunal mucosa to measure immune status and intestinal barrier integrity. Results showed that pigs fed AB diet increased the final body weight and average daily body weight gain and decreased the ratio of feed to gain compared with NC group (P < 0.05). Compared with NC group, AB, AO and ABO decreased serum tumor necrosis factor-α concentration and ABO decreased interleukin-1β concentration in serum and jejunal mucosa (P < 0.05). Compared with NC group, AB up-regulated mRNA expressions of sodium-glucose cotransporte1, claudin-1, occludin and mucin2 in jejunal mucosa and the populations of Bifidobacterium and Bacillus in cecal digesta (P < 0.05). Compared with NC group, ABO increased jejunal mucosal occludin mRNA abundance and Bifidobacterium population in cecal digesta, and decreased Escherichia coli population in cecal digesta (P < 0.05). Furthermore, AB and ABO increased Bacillus population in cecal digesta compared with PC group (P < 0.05). These results indicated that dietary AB supplementation could improve growth performance and intestinal barrier integrity of piglets when fed antibiotic-free diets, which was possibly associated with the improvement of immune status and intestinal microflora. Dietary ABO supplementation is also beneficial to improve immune status and intestinal barrier integrity and microflora of piglets. 相似文献
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The objective of this experiment was to investigate the effect of non-starch polysaccharide (NSP) solubility and inclusion rate on gut health and development, performance and risk of post weaning enteric disorders (PWED) using NSP sources known not to affect digesta viscosity. The experiment consisted of a 2 × 3 factorial combination of NSP type (sNSP vs insoluble NSP (iNSP); inulin vs purified cellulose) and inclusion level (50 vs 100 vs 150 g/kg). Piglets were weaned at 28 days (day 0) and fed ad libitum until slaughter on day 14. There were no incidences of PWED. NSP solubility had little effect on performance, but sNSP diets resulted in lower caecal and colonic pH and higher colonic Lactobacilli to coliform ratio compared to iNSP diets. Increasing NSP levels significantly reduced caecal and colonic pH but decreased empty body weight percentage (P = 0.067). These results suggest that gut health may benefit most from diets containing appropriate sources of predominantly sNSP rather than iNSP. The optimal level of such sNSP will likely depend upon the level of risk of PWED in order to balance the trade off between maximising performance and improving gut health. Effects of NSP feeding under a higher infectious pressure remains to be assessed. 相似文献
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This study examined the impacts of different fiber sources on growth, immune status and gut health in weaned piglets fed antibiotic-free diets. Sixty piglets (BW = 8.18 ± 1.35 kg) were assigned to 3 dietary treatments based on BW and gender in a randomized complete block design (5 replicates/treatment and 4 piglets [2 barrows and 2 gilts]/replicate): (1) an antibiotic-free diet (control, CON); (2) CON + 6% wheat bran (WB); (3) CON + 4% sugar beet pulp (SBP). Dietary WB supplementation tended to increase ADG compared with CON from d 1 to 14 (P = 0.051) and from d 1 to 28 (P = 0.099). Supplementation of WB increased (P < 0.05) G:F compared with CON and SBP from d 1 to 14 and from d 1 to 28. Compared with CON, the addition of WB reduced (P < 0.05) diarrhea rate from d 1 to 14 and tended (P = 0.054) to reduce diarrhea rate from d 1 to 28. The addition of WB decreased (P < 0.05) serum diamine oxidase activity on d 14, and up-regulated (P < 0.05) ileal mRNA levels of occludin on d 28 when compared with CON. Piglets fed WB showed decreased (P < 0.05) serum interleukin-6 levels compared to those fed SBP and decreased (P < 0.05) ileal interleukin-8 levels compared to those fed CON and SBP on d 28. Supplementation of WB increased (P < 0.05) serum levels of immunoglobulin A (IgA), IgG and IgM compared with SBP on d 14, and increased (P < 0.05) the levels of serum IgA and ileal sIgA compared with CON and SBP on d 28. Piglets fed WB showed an enhanced (P < 0.05) α-diversity of cecal microbiota than those fed SBP, while piglets fed SBP showed reduced (P < 0.05) α-diversity of cecal microbiota than those fed CON. Compared with CON, the addition of WB elevated (P < 0.05) the abundance of Lachnospira and cecal butyric acid level. Piglets fed WB also showed increased (P < 0.05) abundances of Lachnospira and unclassified_f_Lachnospiraceae compared with those fed SBP. Collectively, the supplementation of WB to antibiotic-free diets improved performance, immune responses, gut barrier function and microbiota compared with the CON and SBP fed piglets. Therefore, supplementing weaned piglets with WB was more effective than SBP. 相似文献
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本试验通过在日粮中添加低聚木糖探讨其对早期断奶仔猪肠道结构功能的影响。试验选用72头35日龄、体重9.81±0.41 kg杜&#215;长&#215;大三元杂交仔猪,随机分配成4组,每组3个重复,每个重复6头仔猪,公母各半。第1组为对照组,饲喂玉米-豆粕基础日粮;其余3组分别在各组日粮中添加0.01%、0.02%、0.04%低聚木糖作为试验组。试验预饲期2 d,正试期为28 d。于试验的最后1 d从各重复中随机选取1头仔猪屠宰,并测定胃、十二指肠、空肠、回肠、盲肠和结肠内容物的pH及十二指肠、空肠和回肠黏膜上皮的绒毛高度和隐窝深度。试验结果显示,试验组猪的胃及各肠段的pH比对照组均有所降低,尤其是0.02%低聚木糖组,差异显著(P<0.05);试验组各肠段的绒毛高度均高于对照组,差异显著(P<0.05),隐窝深度低于对照组,但差异不显著(P>0.05),绒毛高度与隐窝深度之比显著高于对照组(P<0.05)。本试验结果表明,在早期断奶仔猪的日粮中添加低聚木糖,能降低胃肠道内容物的pH,改善早期断奶仔猪肠道的形态结构,以0.02%低聚木糖组效果最为明显。 相似文献
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Effects of composite antimicrobial peptide on growth performance and health in weaned piglets 下载免费PDF全文
Jiankai Shi Pan Zhang Meng meng Xu Zhengfeng Fang Yan Lin Lianqiang Che Bin Feng Jian Li Gang Li De Wu Shengyu Xu 《Animal Science Journal》2018,89(2):397-403
This study was conducted to evaluate the effects of composite antimicrobial peptide (CAP) on growth performance and health status in weaned piglets. Over 28 days, 36 weaned piglets (body weight, 10.58 ± 0.99 kg) underwent three treatments: negative control (NC, basal diet), positive control (PC, basal diet + 20 mg/kg colistin sulphate + 50 mg/kg kitasamycin), and CAP treatment (CAP, basal diet with 400 mg/kg CAP). Average daily gain of piglets fed the CAP diet was greater (P < 0.05) than that of piglets fed the PC or NC diet during days 1–7, 8–14 and 15–21. Diarrhea rates of piglets fed the CAP or PC diet were lower (P < 0.05) than those of NC‐fed piglets during days 1–7. Apparent total tract digestibility for dry matter and crude ash in CAP‐fed piglets was greater (P < 0.05) than that of NC‐fed piglets. In the CAP group, Lactobacillus and Bifidobacterium counts were greater (P < 0.05) and Escherichia coli counts were lower (P < 0.05) than numbers for the NC group. Our results indicate that dietary CAP had beneficial effects on growth performance and health status in weaned piglets. 相似文献
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Galbreath CW Scholljegerdes EJ Lardy GP Odde KG Wilson ME Schroeder JW Vonnahme KA 《Domestic animal endocrinology》2008,35(2):164-169
Ovariectomized ewes (n=22; 68.76+/-2.34 kg initial body weight; 2.9+/-0.1 initial body condition score) were individually fed one of three diets: (1) control (phytoestrogen-free; n=7), (2) flax containing diet (n=8), or (3) linseed meal (LSM) containing diet (n=7) to investigate the rate of progesterone (P4) clearance. On day 20 of feeding (day 0=initiation of treatment), a P4 releasing device (CIDR) was placed in the vagina and jugular blood samples were obtained prior to CIDR insertion and 15, 30, 60, and 120 min following CIDR insertion. Further, blood samples were obtained daily between days 21 and 24. On day 25, blood samples were retrieved prior to CIDR removal and 2, 5, 10, 15, 30, 60, 120, and 360 min following CIDR removal. There was no difference in initial or final body weight or body condition score and there were no time by diet interactions on P4 clearance. The fractional rate of P4 uptake measured prior to CIDR insertion through day 4 following insertion tended to be greater (P=0.07) in LSM fed ewes (508.75+/-71.37%/min) compared to flax (295.39+/-66.76%/min) and control fed (287.54+/-71.37%/min) ewes. Diet tended (P=0.10) to influence P4 clearance rate when measured from prior to CIDR removal through 120 min following CIDR removal with LSM fed ewes having a greater (1.26+/-0.2) fractional rate constant than flax (0.929+/-0.09) and control fed (0.922+/-0.09) ewes. Flax fed ewes also had more (P<0.01) omega-3 fatty acids and total fatty acids in plasma. Reports of increased pregnancy rates in dairy cows fed flax may relate to P4 metabolism. 相似文献
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酶制剂对仔猪早期断奶后腹泻的影响 总被引:2,自引:0,他引:2
选用44头28±2日龄断奶的仔猪分为对照组和试验组。对照组饲喂基础日粮,试验组饲喂基础日粮+0.l%酶制剂。结果表明,试验组与对照组相比,平均日增重提高了5.88%,平均日采食量降低了4.84%,料肉比降低了10.38%;试验组的腹泻发生率、腹泻频率和死亡率分别降低了4.55%(P>0.05)、28.06%(P<0.05)和66.64%(P>0.05);干物质、粗蛋白和粗纤维的消化率分别提高了3.14%、16.77%和45.14%。提示日粮中添加酶制剂可弥补仔猪早期断奶后内源酶的降低,提高饲料转化率,促进仔猪生长,降低仔猪腹泻发生率和死亡率。 相似文献
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《动物营养(英文)》2020,6(1):47-53
The objective of this study was to compare the effect of linseed and canola expeller on average daily weight gain (ADG), concentrate intakes, incidence of diarrhea, serum haptoglobin, interleukin (IL)-1, and resolvin-E1 in female Holstein calves from birth to weaning. A sample size of 20 calves per group was calculated and were randomly allocated at the time of birth. Linseed group (LIN) was fed a starter with linseed expeller, while canola group (CAN) received a similar concentrate, but with canola expeller. Both expellers were included at a rate of 25% dry matter (DM) basis of the starter. Pasteurized waste milk was fed twice a day until weaning. Calves were weighed at birth, 30, and 60 d of age. Starter intake was evaluated daily from 5 to 60 d. A blood sample was obtained at birth, 14, 28, 35, and 49 d of age, and bovine serum resolvin-E1, haptoglobin, and IL-1 were assayed by commercial enzyme-linked immunosorbent assay (ELISA) kits. Incidence of diarrhea and the duration of the events were also recorded. The effect of the interaction group by time on body weight (BW) and starter intake was not significant (P > 0.05). Average daily gains (ADG) from 0 to 60 d for CAN and LIN groups were 0.680 and 0.675 kg/d (P > 0.05), respectively. Incidences of diarrhea were 25% and 45% for CAN and LIN groups, respectively (P = 0.18). LIN group had greater concentrations of IL-1 at d 21, haptoglobin at d 7, and resolving-E1 at d 14 and 49 than CAN group, respectively. It is concluded that BW at weaning, ADG, and concentrate intakes were not different between groups fed starters containing linseed or canola expeller (25% inclusion). The concentrations of cytokines and haptoglobin were the greatest in LIN group. 相似文献
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The influence of heated mash on growth and feeding behavior of newly weaned piglets was investigated. An automatically ventilated nursery with 4 identical pens was used. Twenty piglets weaned at 21 d were housed in each pen. The experiment was repeated 3 times. In total, data were obtained from 240 piglets of 12 pens. The pens were provided with a sensor-controlled, automatic feeding device, which dosed a ready-mixed mash in a trough. In each of 2 of the pens, the feed was mixed with warm water at 36 degrees C, during the first week of weaning. This heated mash had a temperature of 34 degrees C at the outlet of the automatic feeding device (experimental group). In the 2 control groups, the water was not heated and the temperature of the mash was 14 degrees C at the outlet of the automatic feeding device. From the second week of weaning, the mash had a temperature of 14 degrees C at the outlet of the automatic feeding device in all 4 pens. Piglets were weighed at weaning, at weekly intervals through 49 d after weaning, and on d 139 after weaning. Behavior of the whole group, as well as behavior of selected focal animals, was evaluated for the first 48 h after weaning. In addition, skin condition of piglets was assessed on day of weaning and on d 7, 14, and 21 after weaning. The amount of feed consumed by the piglets was recorded on a daily basis throughout the whole period of nursery. Over the total period of the study, piglets in the experimental group gained 3.98 +/- 1.66 kg (P = 0.047) more than the control group. The difference was particularly clear during the nursery period (49 d) when the experimental group gained 0.89 +/- 0.23 kg more than the control group (P = 0.03). Although piglets in the control group consumed 37.15 +/- 0.15 kg of feed over the complete nursery period, the experimental group consumed 42.56 +/- 0.15 kg per piglet (P = 0.023). By heating the mash feed in the first week after weaning, both growth performance as well as feed consumption of piglets could be increased. No difference in feed conversion and feeding behavior was found between groups. 相似文献
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Rafael A Cabrera James L Usry Consuelo Arrellano Eduardo T Nogueira Marianne Kutschenko Adam J Moeser Jack Odle 《畜牧与生物技术杂志(英文版)》2013,4(1):29
Background
Creep feeding is used to stimulate piglet post-weaning feed consumption. L-Glutamine (GLN) is an important source of fuel for intestinal epithelial cells. The objective of this study was to determine the impact of creep feeding and adding GLN or AminoGut (AG; containing glutamine + glutamate) to pre- and post-weaning diets on pig performance and intestinal health. Litters (N = 120) were allotted to four treatments during 14–21 d of lactation: 1) No creep feed (NC, n = 45); 2) creep fed control diet (CFCD, n = 45); 3) creep fed 1% GLN (CFGLN, n = 15); 4) creep fed .88% AG (CFAG, n = 15). After weaning, the NC and CFCD groups were sub-divided into three groups (n = 15 each), receiving either a control nursery diet (NC-CD, CFCD-CD) or a diet supplemented with either GLN (NC-GLN, CFCD-GLN) or with AG (NC-AG, CFCD-AG). Litters that were creep fed with diets containing GLN or AG also were supplemented with those amino acids in the nursery diets (CFGLN-GLN, CFAG-AG). Glutamine was added at 1% in all three post-weaning diet phases and AG was added at .88% in phase 1 and 2 and at .66% in phase 3.Results
Feed conversion (feed/gain) showed means among treatment means close to significance (P = 0.056) and Tukey’s test for pairwise mean comparisons showed that Pigs in the CFGLN-GLN group had the best feed conversion (feed/gain) in the first three-week period post-weaning, exceeding (P = 0.044) controls (CFCD-CD) by 34%. The NC-AG group had (P = 0.02) the greatest feed intake in the last three week of the study, exceeding controls (CFCD-CD) by 12%. CFGLN-GLN, CFCD-GLN and sow reared (SR) pigs had the greatest (P = 0.049) villi height exceeding the CFCD-AG group by 18%, 20% and 19% respectively. The CFAG-AG group had the deepest (P = 0.001) crypts among all treatments. CFGLN-GLN, CFCD-GLN and SR groups had the greatest (P = 0.001) number of cells proliferating (PCNA) exceeding those in the NC-CD group by 43%, 54% and 63% respectively. Sow reared pigs showed the greatest (P = 0.001) intestinal absorption capacity for xylose and mannitol.Conclusion
Supplementation of creep feed and nursery diets with GLN and/or AminoGut in the first three week improved feed conversion possibly due to improved intestinal health. 相似文献20.
喷雾干燥破膜血球蛋白粉替代血粉对断奶仔猪生长性能的影响 总被引:1,自引:0,他引:1
试验采用单因子试验设计,选用60头(28±2)日龄断奶,体重(7.08±0.82)kg二元杂交仔猪,随机分为3组,每组5个重复,每个重复4只猪,分别用0、1.76%、3.52%喷雾干燥破膜血球蛋白粉(SDBC)取代仔猪基础日粮(玉米-豆粕型,添加4%的血粉)中血粉的0、50%、100%,研究SDBC部分或全部替代血粉对断奶仔猪生长性能、养分消化率和血液指标的影响。经过28d的试验,结果表明:与对照组相比,1.76%SDBC组和3.52%SDBC组均可显著提高仔猪ADG、降低F/G。而且1.76%SDBC组的ADG和F/G分别比对照组提高了24.7%和降低了14.1%,其养分消化率也最高。由此可见,SDBC能够完全替代血粉,但以添加1.76%SDBC替代50%的血粉效果最好。 相似文献