生根阶段组培微环境变化对蝴蝶兰组培苗驯化的影响

在生根阶段对蝴蝶兰组培苗进行不同蔗糖、CQ浓度和光照处理后，影响蝴蝶兰组培苗的驯化过程的植株生长和光合生理。研究表明，提高组培环境中CQ浓度有效改善组培苗的光合能力利于组培苗的移栽适应性。     

Promotive effects of a 5-aminolevulinic acid-based fertilizer on growth of tissue culture-derived date palm plants (Phoenix dactylifera L.) during acclimatization

The low survival rate together with the slow growth rate of the survived tissue culture-derived date palm plants during acclimatization are critical problems facing the tissue culture as a commercial technique of date palm propagation. Growth parameters, pigment contents and nutrient uptake were examined in tissue culture-derived date palm plants (Phoenix dactylifera L.) c.v. ‘Khalas’ subject to different concentration of a 5-aminolevulinic acid-based fertilizer, commercially known as Pentakeep-v, during acclimatization. Pentakeep application at 0.02, 0.04 or 0.08% at 10 days interval significantly enhanced growth at both VP2- and VP3-stage of acclimatization. However, such effects were not detectable at the VP1-stage. At the end of this stage, the successful plants ranged from 66.6 to 78.3% with no significant differences between the Pentakeep treatments and the control. All successful plants at this stage survived both the VP2 and the VP3 stages of acclimatization. Plants treated with Pentakeep, especially at 0.04 and 0.08%, developed faster and were ready for transplanting to the nursery or to the open field conditions about 4–5 months earlier than the control or those treated with 0.02% Pentakeep. Pentakeep significantly increased chlorophyll a contents in all treatments which was subsequently reflected on total chlorophyll and chlorophyll a/chlorophyll b ratio. Pentakeep application, especially at the 0.08%, significantly increased the concentration of N, Cu and Zn in the leaves of treated plants. The concentration of available P and Zn in the soil was significantly higher in the Pentakeep treatments than the control.     

葡萄试管苗在移栽过程中叶解剖和根系活力的变化

葡萄试管苗通过分步驯化后,能够高频率的成活。观察和测定了各驯化阶段叶解剖结构和根系活力的变化,结果表明,试管苗叶片薄,组织稀疏,气孔突起,开口大,根系活力极低。通过分步炼苗,叶片逐渐向旱生结构变化,根系活力迅速增强。这是试管苗逐渐适应低湿和自然光照的解剖和生理变化。     

金合欢组织培养和快速繁殖的研究

 采用金合欢(Acacia dealbata) 大树顶芽、幼苗茎尖、种子小苗上胚轴和幼叶作外植体进行组培和快繁研究。结果表明: 用MS + 6-BA 6 mg/L + NAA 0. 1 mg/L + NaH₂PO₄ 30 mg/L 可提高愈伤组织的出芽率, 有利于扩大繁殖系数, 而用MS + 6-BA 2 mg/L + NAA 0. 1 mg/L + NaH₂PO₄ 30 mg/L 可以增加发枝率。珍珠岩+ 泥炭(1∶1) 基质可促进小苗后期生长,加速成苗。     

平欧杂交榛的组织培养

为了探索我国平欧杂交榛离体培养和快繁技术,以5个平欧杂交榛品种(系)的幼嫩茎段为外植体,研究其组织培养及快速繁殖技术,结果表明,(1)5月中旬为茎尖培养取材的较好时期,污染率低、萌芽率高。(2)适宜的基本培养基为DKW培养基。(3)不同基因型间的萌芽率及芽苗生长状态存在极显著差异。(4)增殖阶段较好的培养基为:DKW+TDZ1.5mg/L+IBA0.01mg/L。(5)生根培养较佳培养基为:1/2MS+NAA0.1mg/L,生根率达90%。     

Light quality regulates shoot cluster growth and development of MM106 apple genotype in in vitro culture

The interaction between shoot proliferation and regulative action of light quality was investigated in terms of cluster growth and development. In the commercial laboratory, the shoot multiplication procedure is normally conducted by growing clusters of shoots during several sub-cultures. Thus in this work the regulative action of light quality was evaluated by analysing the shoot cluster proliferation rate, the evolution of axillary bud differentiation, the axillary shoot development on both initial explant stem (order 1 axis) and three representative lateral branches (order 2 axes), growing from the lateral buds located at the bottom of the initial explant. Experiments showed that light qualities regulate bud differentiation and interact with apical dominance, leading to strong control of axillary bud outgrowth, and, finally, resulting in a differentiated architectural formation of clusters and type of branching.     

红叶石楠组培苗生根培养的研究

探讨了激素处理组培苗方法、IBA浓度、浸蘸时间、培养基及活性炭对诱导红叶石楠组培苗生根的影响,筛选出最佳的生根诱导方法是采用浸蘸100 mg/kg IBA 60 min后接种于添加0.3%活性炭的1/2 MS中,生根率达到90%,平均生根数可达到5.23条.     

Damas 1869李组培繁殖技术研究

以Damas1869李腋芽为材料,研究了不同消毒方法对冬季水培芽及春季田间芽污染率、存活率的影响。采用3因素2水平正交实验,研究不同激素配比对芽增殖的影响,不同浓度的IBA、NAA对生根的影响。结果表明,与春季田间芽相比以冬季水培芽灭菌效果较好,即以75%酒精浸洗10s,再用20%次氯酸钠浸洗8min,存活率为64.1%;最佳芽增殖培养基是MS+6-BA1.0mg/L+NAA0.5mg/l+20g/L蔗糖+5g/L琼脂粉,增殖系数最高可达7.1;适宜生根的培养基是1/2MS+IBA2.0mg/L+20g/L蔗糖+5g/L琼脂粉+0.05%活性炭,生根率为87.8%,平均生根数3.9。     

锥栗植株再生体系的建立

【目的】为了建立锥栗再生体系,【方法】以锥栗胚为外植体,分析不同基因型、基本培养基及激素组合对锥栗不定芽的诱导、增殖和生根的影响。【结果】结果表明,油榛是锥栗组织培养最佳的基因型;锥栗不定芽诱导的最适培养基为M(NH4NO3804 mg·L-1+KNO31011 mg·L-1+FeSO4.H2O 27.8 mg·L-1+1/2MS其余无机盐+MS有机)+6-BA 2.0mg·L-1+IBA 0.2 mg·L-1,其出芽率为76%;芽增殖的最佳培养基为M(同上)+6-BA 0.5 mg·L-1+IBA 0.1 mg·L-1,其增殖倍数达18;诱导生根的最佳培养基为M(同上)+IBA 0.5 mg·L-1,其生根率为48%。【结论】运用上述体系可获得完整锥栗再生植株。     

甜瓜组织培养及遗传转化研究进展

概述了甜瓜组织培养过程中外植体类型、培养基组成、添加物等主要因素对植株再生的影响,以及甜瓜体细胞胚状体发生途径、单倍体、原生质体培养的研究现状和根癌农杆菌介导甜瓜遗传转化的研究进展。     

组培实验室常见消毒方法优劣比较

通过介绍比较组培实验室环境消毒和外植体消毒几种常见方法的原理、注意事项及优缺点等,认为用中草药及新洁尔灭进行环境消毒更为低毒友好;对外植体进行消毒时,综合考量,次氯酸钠比氯化汞更为合适。     

苹果离体叶片再生体系两步培养法的研究

通过对苹果离体叶片两步再生法的研究,建立了乔纳金苹果试管苗的叶片再生体系。试验表明,2, 4-D能促进愈伤组织的形成和增殖,影响外植体脱分化和再分化的趋向。BA影响外植体形成愈伤组织的能力,对不定芽再生具有显著影响。IAA对愈伤组织的形成几乎无影响,但对再生不定芽的生长影响较大。诱导愈伤组织的培养基为MS+BA 1 0mg·L-1 +IAA 0 2mg·L-1 +2, 4 -D 0 8mg·L-1时诱导率可达100%;分化不定芽的培养基为MS+BA4 0mg·L-1 +IAA0 2mg·L-1时最高分化率也达 100%。暗培养 1月左右即可得到再分化的不定芽。     

影响文心兰原球茎增殖生长诸因素的研究

以文心兰茎尖为外植体,诱导原球茎,并对影响原球茎增殖的几种因素进行了研究.结果表明:文心兰茎尖在1/2 MS+BA 2 mg/L+NAA 0.3 mg/L培养基上,离体培养大约30 d后产生原球茎;原球茎在1/2 MS+BA 1 mg/L+NAA 0.2 mg/L培养基上增殖速度最快,增殖系数可达2.6;与果糖和葡萄糖相比,蔗糖更有利于原球茎增殖;30 g/L的蔗糖对原球茎增殖生长有促进作用.     

Three dimensional tissue culture in culture medium with rhbFGF

AIM:To investigate the behaviour of 3T3 fibroblast and macrophage co-culture on blood fibrin clot or adipose tissue with recombinant basic fibroblast growth factor (rhbFGF).METHODS:MTT method, inverted contrast microscopy, Giemsa staining as well as scanning electron microscope were used in the present study.RESULTS:The effect of rhbFGF on co-culture of 3T3 fibroblast and mouse macrophage on blood fibrin clot in low-serum DMEM with rhbFGF were monitored, and 3T3 fibroblast and macrophage growed well on the blood fibrin blot in low-serum DMEM with rhbFGF. CONCLUSION:The blood fibrin clot, with its low immunogenicity, could be used as a bionic support for three-dimensional tissue culture, and also a physiological carrier to distribute the growth factor rhbFGF for the cells.     

瓜类蔬菜组织培养与遗传转化研究进展

概述了瓜类蔬菜组织培养过程中的主要影响因素—基因型、外植体类型,部位与生理状态、培养基、生长调节物质、碳源及其他添加物质对植株再生的影响,以及根癌农杆菌介导的瓜类蔬菜遗传转化的影响因素与研究进展。     

钙果组织培养育苗技术研究

以农大钙果3、4、5号品种为研究对象,研究其组织培养及快速繁殖技术. 结果表明:①以钙果幼嫩茎段的侧芽为外植体,消毒效果最好.②以MS为基本培养基,初代培养附加0.4mg/L6-BA和0.4mg/LIAA腋芽萌发最好;增殖培养附加0.4mg/L6-BA和0.5mg/LIAA腋芽增殖效果最佳.③生根培养阶段,以1/2MS为基本培养基,附加0.5 mg/L IBA生根效果最好.④选用山土作为钙果试管苗的移栽基质效果最佳.     

植物种质资源离体保存技术研究进展

植物种质资源的保存对于生物多样性保护和新品种选育均具有十分重要的意义。综述了国内外植物种质资源离体保存技术最新研究进展,并提出了有待继续研究的相关问题。     

钙果4号欧李组织培养技术研究

以钙果4号欧李秋季生长的幼嫩上部枝和春季萌生的幼嫩基生枝为材料,利用正交试验设计等方法研究了欧李组织培养的最佳基本培养基和植物生长调节剂配比。结果表明,以春季萌生的幼嫩基生枝为外植体材料较好,以70%～75%酒精浸洗10～30s后用0.1%升汞浸洗6min消毒效果最好,适宜芽诱导的培养基是改良MS+NAA0.1mg/L+6-BA1.0mg/L+20g/L蔗糖+7g/L琼脂,最佳芽增殖培养基是改良MS+6-BA2.0mg/L+NAA0.02mg/L+GA30.2mg/L+20g/L蔗糖+7g/L琼脂,适宜生根培养基是1/2MS+IBA1.0mg/L+KT0.02mg/L+20g/L蔗糖+7g/L琼脂。     

贵州地宝兰组培快繁生根技术研究

为促进贵州地宝兰的组织培养快繁及工厂化育苗技术的商业推广,以野生贵州地宝兰优良种子苗的侧芽为材料,研究不同基本培养基组合、不同植物生长调节剂对贵州地宝兰侧芽生长及生根的影响。试验结果表明,最适宜贵州地宝兰侧芽生根的基本培养基是N6,其次是B5。最适生根的生长调节剂组合是2mg/LBA+2mg/LNAA,培养周期为100d。     

西瓜离体再生高效基因型材料的筛选

为完善西瓜离体再生体系,以23种基因型材料的西瓜无菌苗子叶为试材,运用方差分析筛选高效基因型材料及芽诱导最适培养基。结果表明:基因型不同的材料,诱导效果有明显差异。筛选的再生高效基因型为鲁圆、三白、FW351、PM2和京母。鲁圆诱导效果最好,最高诱导率达到90.0%,与18种基因型材料的诱导差异达到了显著水平;三白、FW351、PM2和京母的诱导效果次之,诱导率分别为85.0%、82.5%、82.5%和80.0%。筛选的芽诱导最适培养基为MS+6-BA3.0mg/L。