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植物乳杆菌ST-Ⅲ(Lb.pzantarum ST-Ⅲ)是一株具有降低血清胆固醇能力的益生菌,其在脱脂乳中生长缓慢,产酸能力弱.实验选取一株凝乳性较好的植物乳杆菌CZ 2112作为参照,对植物乳杆菌ST-Ⅲ蛋白酶活性进行研究.植物乳杆菌ST-Ⅲ蛋白酶活性及蛋白水解能力比植物乳杆菌CZ 2112低,而氨肽酶和羧肽酶活性与之差异较少,推测得出植物乳杆菌ST-Ⅲ在脱脂乳中不能很好生长和凝乳的原因是该菌株蛋白酶活性较低,生长初期不能很好利用外源蛋白质营养.在添加鱼蛋白胨等促进生长后,植物乳杆菌ST-Ⅲ可以将蛋白质、多肽水解成一系列的寡肽或氨基酸,满足菌体生长的氮素营养. 相似文献
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植物乳杆菌ST-Ⅲ对实验性动物高胆固醇血症影响的研究 总被引:2,自引:0,他引:2
在建立试验用高胆固醇血症大鼠模型的基础上,观察用植物乳杆菌ST-Ⅲ制成的三个不同剂量的ST-Ⅲ活性乳对大鼠血脂水平的影响。动物实验表明:三个剂量的ST-Ⅲ活性乳均可显蓍降低大鼠血清胆固醇(TC)的含量(P<0.01),但对甘油三酯(TG)的降低作用不显著(P>0.05)。高剂量的ST-Ⅲ活性乳可显著提高大鼠血清高密度脂蛋白胆固醇(HDL-c)含量(P<0.01)。因此认为ST-Ⅲ活性乳具有辅助降血脂的功能,特别对高胆固醇血症具有良好的功效。 相似文献
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近10 年来,随着先进研究方法的使用和研究内容的不断深入,对益生菌的认识取得重大进展,尤其是对益生菌促进健康作用的机理逐渐清晰,益生菌制备技术和产业化技术更加成熟,为新型发酵乳的开发提供了坚实的发展基础。本文对益生菌功能机理、遗传转化及代谢调控的研究现状和发展进行阐述,介绍分离自内蒙古马乳酒中的干酪乳杆菌LC2W和分离自发酵蔬菜中的植物乳杆菌ST-Ⅲ的益生特性、基因组及产胞外多糖特性,并进一步探讨基于植物乳杆菌增殖技术开发含高活菌数的纯植物乳杆菌发酵乳品,以期为益生菌和功能发酵乳的应用开发提供理论依据。发酵乳品是益生菌的良好载体,吸收现代益生菌科技成果,加强循证医学方法论证,基于生物活性效应分子及生物活性评价,增加临床研究数据,新型发酵乳将为人类健康做出更多贡献。 相似文献
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本试验通过在MRS液态培养基中添加不同浓度的腐植酸钠,比较植物乳杆菌生长、代谢及形态变化,初步探讨了液态条件下腐植酸钠对植物乳杆菌的抑制作用。结果表明,1%~5%腐植酸钠降低了植物乳杆菌的活菌数,但可提前6h达到生长最高点;3%腐植酸钠作用后,植物乳杆菌的有机酸产量发生了改变,且菌落变小,菌体变粗;透射电镜结果表明腐植酸钠使得植物乳杆菌细胞壁和细胞膜结构部分变得模糊。因此,液态条件下腐植酸钠通过改变植物乳杆菌的代谢途径、形态结构达到抑制其生长的作用。 相似文献
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Kensuke ARAKAWA Yasushi KAWAI Kenji FUJITANI Junko NISHIMURA Haruki KITAZAWA Ken-ichi KOMINE Kenzo KAI Tadao SAITO 《Animal Science Journal》2008,79(5):634-640
The use of bacteriocins from Lactobacillus gasseri , a probiotic lactic acid bacterium, as bio-preservatives in the food industry and animal formulations has been limited because few strains of Lb. gasseri are cultivated and produce a bacteriocin in natural media such as milk and milk-based media. By the determination of the growth-supplements to milk among the 47 nutrients, Lb. gasseri JCM1131T , LA39 and LA158 isolated from human feces were successfully cultured in reconstituted skim milk and cheese whey using proteose peptone as a nutrient supplement, where Lb. gasseri LA39 produced a useful bacteriocin, gassericin A, with effective growth-inhibiting activity against Gram-positive food-borne pathogens. The data suggest these developed low-cost safe media supporting enough production of bacteriocins by the probiotic Lb. gasseri LA39 could be used to improve the safe bio-preservation of foods and therapy of bovine mastitis, and extra cheese whey produced by cheese making industry is reused in the cultivation for probiotics effectively. 相似文献
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以33株具有潜在抑制真菌活性的植物乳杆菌为研究对象,分别与基础发酵剂复配制备酸乳,通过测定发酵酸乳在敞口室温条件下贮藏期间霉菌、酵母菌的菌数变化及酸乳的酸度,筛选具有良好保鲜效果的植物乳杆菌.结果表明:初筛所得9株植物乳杆菌(IMAU80152、10156、80091、50045、10155、80106、10216、10996、10239)可明显抑制真菌生长,复筛出IMAU80091和IMAU80106在酸乳中酸度变化程度显著低于其他菌株及商业保鲜菌(P<0.05),当上述2株菌以1∶1复配发酵时抑制酵母菌效果显著优于商业保鲜菌(P<0.05).复配发酵的酸乳样品贮藏21d后,滴定酸度为(138.24±1.19)°T,显著低于商业保鲜菌组(146.41±1.18)°T(P<0.05).IMAU80091和IMAU80106复配组合可有效抑制货架期内酸乳真菌污染,具有作为生物保鲜菌的优良特性. 相似文献
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Classification of lactic acid bacteria isolated from chigee and mare milk collected in Inner Mongolia 总被引:1,自引:0,他引:1
One hundred and seventeen isolates from chigee and 191 isolates from mare milk collected in Inner Mongolia were classified into six and 10 groups, respectively, based on the protein profiles obtained by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). A dendrogram on the basis of the molecular sizes of the protein bands was drawn and the similarity among the groups ranged from 96% to 44%. Representative isolates from all groups were identified by 16S rDNA sequencing and a phylogenetic tree was drawn. Furthermore, the results of carbohydrate fermentation tests of all groups were compared with those obtained by 16S rDNA sequencing and SDS‐PAGE analyzes. The isolates from chigee consisted of mainly Lactobacillus sp., Lactobacillus (Lb.) plantarum, Lb. pentosus and Lactococcus (Lc.) lactis ssp. cremoris were isolated at the rates of 48, 33 and 19%, respectively. The isolates from mare milk consisted of coccus, namely, Leuconostoc (Leuc.) mesenteroides, Leuc. pseudomesenteroides, Lc. garvieae, Lc. lactis ssp. lactis, Streptococcus (Sc.) parauberis and Enterococcus (Ec.) faecium. The isolation rates were 45, 19, 7, 8, 16 and 6%, respectively. The results demonstrated that the species isolated from chigee were different from those in mare milk. 相似文献
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为改善酸羊乳风味与品质,提高酸羊乳的功能价值,将自浆水中筛选的植物乳杆菌JS5和JS19用于羊乳发酵,研究其对酸羊乳理化性质、质构特性及功能特性的影响。结果表明:2 种植物乳杆菌与商业发酵剂复合发酵后,均能在贮藏期内有效提高酸羊乳持水性和表观黏度,降低其硬度与胶着性,促进乙醛和双乙酰的释放;添加植物乳杆菌JS5的酸羊乳胆固醇降解率显著高于对照组(P<0.05),最高可达36.62%;添加植物乳杆菌JS19的酸羊乳体外抗氧化能力更强,贮藏1 d时1,1-二苯基-2-三硝基苯肼自由基清除率高达70.58%,铁离子还原/抗氧化能力达32.03 μmol/L。2 种菌株均为适用于羊乳发酵生产的优良菌株。 相似文献
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为提高植物乳杆菌XN1904E胞外多糖产量,考察培养温度、培养基初始pH值、碳源和氮源对植物乳杆菌XN1904E胞外多糖产量的影响。通过单因素试验和正交试验确定植物乳杆菌XN1904E产胞外多糖的最佳培养条件。结果表明:最佳培养条件为培养基初始pH 6.5、培养温度37 ℃、半乳糖为最适碳源、鱼蛋白胨为最适氮源,此条件下验证实验结果表明,植物乳杆菌XN1904E胞外多糖产量为235.41 mg/L;植物乳杆菌XN1904E胞外多糖水溶液(0.2 mg/mL)对1,1-二苯基-2-三硝基苯肼自由基的清除率为27.54%,表明其具有一定的抗氧化能力。 相似文献
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Montel Mendoza G Pasteris SE Ale CE Otero MC Bühler MI Nader-Macías ME 《Research in veterinary science》2012,93(3):1160-1167
The cultivable microbiota of skin and cloaca of captive Lithobates catesbeianus includes microorganisms generally accepted as beneficial and potentially pathogenic bacteria. In order to select a group of potentially probiotic bacteria, 136 isolates were evaluated for their surface properties and production of antagonistic metabolites. Then, 11 lactic acid bacteria (LAB) strains were selected and identified as Lactobacillus plantarum, Lb. brevis, Pediococcus pentosaceus, Lactococcus lactis, L. garvieae and Enterococcus gallinarum. Studies of compatibility indicate that all the strains could be included in a multi-strain probiotic, with the exception of Ent. gallinarum CRL 1826 which inhibited LAB species through a bacteriocin-like metabolite. These results contribute to the design of a probiotic product to improve the sanitary status of bullfrogs in intensive culture systems, to avoid the use of antibiotics and thus to reduce production costs. It could also be an alternative to prevent infectious diseases during the ex situ breeding of amphibian species under threat of extinction. 相似文献
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蚕蛹制取试剂蛋白胨研究 总被引:2,自引:0,他引:2
蚕蛹通过酶水解制取无臭、淡黄的试剂蛋白胨。75kg脱脂蚕蛹以6kg中性蛋白酶粗品在pH7—8、40—50℃、水解10小时,随后分离、脱色、浓缩和喷雾干燥,得到约30kg蛋白胨。产物经11项理化检验和3项微生物检验,结果表明达到了生化试剂蛋白胨的质量要求。将蚕蛹蛋白胨应用于氨基糖苷类抗菌素小诺霉素、肌苷和复合核黄素(维酶素)等生产菌发酵,效果同以鱼粉制取的试剂蛋白胨相当。 相似文献
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以益生菌植物乳杆菌LIP-1为研究对象,MRS基础培养基为对照,通过改变培养基的成分及其含量(碳源、氮源、碳氮总量及碳氮比、缓冲盐),研究培养基成分的变化对植物乳杆菌LIP-1高密度培养的活菌数以及冷冻干燥后存活率的影响。结果表明:通过优化工艺和配方确定的最佳培养基组成为碳源(蔗糖)添加量33.35 g/L、复合氮源(酵母粉、大豆蛋白胨、酪蛋白胨质量比3∶3∶5)添加量10.80 g/L、缓冲盐(乙酸钠、磷酸氢二钾、柠檬酸钠质量比2.5∶1∶1)添加量14.85 g/L(即0.13 mol/L);用优化后 相似文献