北方蚕区2012年春季桑蚕新品种实验室鉴定（陕西点）报告

2012年春季对山东省蚕业研究所、山东农业大学、河南省蚕业科学研究院、陕西省蚕桑丝绸研究所4个单位的6对桑蚕新品种进行了实验室鉴定,调查其蚕期饲育表现、茧质、经济性状,总结参鉴品种在陕西试验点的性状表现。     

北方蚕区2013年春季桑蚕新品种实验室鉴定（陕西点）报告

2013年春季陕西试验点对北方蚕区4家单位选育的的6对桑蚕新品种进行了实验室鉴定。以菁松X皓月作为对照种,观察参鉴品种的性状表现,调查龄期经过、体质、茧质、收茧量等经济指标。本次鉴定结果显示,各参鉴品种的蚕期性状表现良好,鲁51×鲁52、ZHG×春54、ZHG×秋54这3对品种的经济性状优良。     

2009年春季北方蚕区桑蚕新品种实验室鉴定(陕西点)报告

2009年春季依照《北方蚕区桑蚕品种共同鉴定工作细则》对4对桑蚕新品种进行了实验室鉴定,主要从蚕期饲育观察、茧质调查、丝质鉴定三方面作对比分析,客观地反映这几对品种在陕西的性状表现。     

北方蚕区2015年春季桑蚕新品种实验室鉴定（安康点）报告

2015年春，对山东省蚕业研究所、山东农业大学、陕西省蚕桑丝绸研究所3个单位的4对桑蚕品种进行了实验室鉴定。结合蚕期表现、茧期调查、丝质鉴定等，总结参鉴品种在安康试验点的饲育情况和性状特点。     

桑蚕新品种华康2号广东实验室鉴定报告

2014年-2015年秋,广东省蚕业技术推广中心对参加国家桑蚕品种鉴定的桑蚕新品种华康2号进行了两年实验室鉴定.通过蚕期饲养表现、茧质调查成绩、丝质鉴定成绩及综合经济性状的对比分析,客观反映了华康2号在广东的饲养情况及性状表现,为国家蚕品种审定和推广提供科学、客观的依据.     

北方蚕区2015年春季桑蚕新品种实验室鉴定(山东点)报告

2015年春季,山东蚕区试验点对来自北方蚕业科研协作区4家育种单位的5对桑蚕品种进行了实验室鉴定试验.以现行生产用种菁松×皓月为对照种,结合试验室蚕期饲养、茧期调查、缫丝检验,总结分析各试验蚕品种在山东蚕区试验点的性状表现、蚕期饲育特点及茧丝质综合成绩,并进行简要的评价和分析.     

北方蚕区2014年春季桑蚕新品种实验室鉴定（安康点）报告

2014年春,对山东省蚕业研究所、山东农业大学、安康市蚕桑产业发展中心3个单位的3对桑蚕品种进行了实验室鉴定。结合蚕期表现、茧期调查、丝质鉴定,总结参鉴品种在安康试验点的饲育情况和性状特点。     

北方蚕区2014年春季桑蚕新品种实验室鉴定（河南点）报告

2014年春季,河南省蚕业科学研究院对鲁51×鲁52、优食一号这2对桑蚕品种进行了实验室联合鉴定。结合蚕期饲养、茧期调查、丝质鉴定,观察和总结这2对品种在河南试验点的饲育表现和性状特点。     

北方蚕区2016年春季桑蚕新品种实验室鉴定(安康点)报告

2016年春,对山东农业大学的1对桑蚕品种进行了实验室鉴定.结合蚕期表现、茧期调查、丝质鉴定等,总结参鉴品种在安康试验点的饲育情况和性状特点.     

2017年春季北方蚕区桑蚕新品种实验室鉴定 (安康点)报告

2017年春季,对桑蚕品种苏婴×熙阳进行了实验室鉴定.结合蚕期表现、茧期调查、丝质鉴定等,总结参鉴品种在安康试验点的饲育情况和性状特点.     

IMS-PCR对食品中单增李斯特菌快速检测

为了建立一种快速检测食品中单增李斯特菌的方法,试验设计了Listeriolysin O、23SrRNA和hly、iap四对引物分别做双重PCR引物,并与免疫磁珠法分离LMO相结合对LMO进行快速鉴定。结果显示,这四对引物分别扩增条带为700bp,240bp,308bp,505bp,可分辨不同种属的李斯特菌和其它不同菌株。建立的IMS-PCR方法最低检测限可达到1CFU/25g（mL）食品,通过检测食品样本188份,结果与常规检测方法所得的结果一致率为100%。所以,建立的IMS-PCR方法具有极好的特异性、敏感性和稳定性,在24h内可得到准确的检测结果,具有广阔的发展和市场应用前景。     

四种叶形吸虫的染色体组型分析

应用一种自创小型复殖吸虫染色体制备法，对四种常见寄生鱼类的叶形吸虫（中华叶形吸虫、鳗鲡叶形吸虫、鲶叶形吸虫和巴氏叶形吸虫）的染色体组型分析表明，它们的有丝分裂中期染色体二倍体数目均为２ｎ＝１８，且核型十分接近，即由四对较长的具近端着丝点染色体，四对较短的具近中部着丝点染色体及一对具中部着丝点的短染色体组成。但是，它们在染色体相对长度方面存在一定的差异。聚类分析显示，中华叶形吸虫和鳗鲡叶形吸虫，鲶叶形吸虫和巴氏叶形吸虫分别组成一近缘类群。根据发状科各属虫种的染色体组型资料，探讨了该科吸虫的核型进化问题。     

DNA fingerprint patterns of Pasteurella multocida from the same turkey farm on the same and different years.

The DNA fingerprint profiles of 126 isolants of Pasteurella multocida from 41 turkey farms in Missouri were analyzed after digestion with the restriction endonuclease HhaI and compared with their somatic antigenic type. The goal was to determine if the same isolant of P. multocida was reisolated from the the same farm during the same and consecutive years and after an interval of one or more years. Of the 37 pairs of P. multocida collected during the same year from the same turkey farms, the DNA fingerprint profiles were the same with 26 pairs (70.3%) and different with 11 pairs (29.7%). Of the 33 pairs of P. multocida collected during consecutive years from the same 22 turkey farms, 21 pairs (63.6%) were the same and 12 pairs (36.4%) were different. Of the 15 pairs of P. multocida collected with an interval of one or more years between them from the same 14 turkey farms, only four pairs (26.7%) were the same and 11 pairs (73.3%) were different. There did not appear to be any relationship between the DNA fingerprint profiles and the typing of their somatic antigens because, although 44 pairs of isolants had the same DNA fingerprint profile and somatic antigenic type, 42 pairs differed in these parameters when all pairs were combined.     

Characterisation of a Cryptosporidium isolate from water buffalo (Bubalus bubalis) by sequencing of a fragment of the Cryptosporidium oocyst wall protein gene (COWP)

Cryptosporidium oocysts were detected using a direct immunofluorescence antibody test in the faeces of an asymptomatic water buffalo (Bubalus bubalis) heifer from a dairy farm close to Santiago de Compostela (NW Spain). Oocysts were morphologically indistinguishable from Cryptosporidium parvum. Using DNA extracted from this sample and a PCR-RFLP analysis of a 341 base pairs fragment of the Cryptosporidium oocyst wall protein (COWP) gene, a previously undescribed fragment pattern was generated. The COWP gene fragment was cloned and sequencing analyses revealed it to be similar to the C. parvum 'pig' genotype but with four base pairs substitutions.     

Vitamin A requirements of alipochromatic ('recessive-white') and coloured canaries (Serinus canaria) during the breeding season

Six pairs of alipochromatic ('recessive-white') canaries (Serinus canaria) and six pairs of coloured canaries were kept through a complete breeding cycle while being fed a diet providing 12,000 iu vitamin A/kg. The eggs of three pairs (one recessive-white and two coloured) were all unfertilised and there were only 23 hatchlings (14 recessive-white and nine coloured), of which 14 (10 recessive-white and four coloured) were alive after the first moult. However, there was no clinical, biochemical or pathological evidence that the recessive-white canaries were suffering from vitamin A deficiency or that the coloured canaries were suffering from vitamin A toxicity, suggesting that the diet met the vitamin A requirements of both groups.     

猪繁殖与呼吸综合征病毒一步多重RT-PCR检测方法的建立及应用

本研究旨在建立猪繁殖与呼吸综合征病毒(PRRSV)的一步多重RT-PCR检测方法.通过多序列比对以及反应条件的优化,从4对引物中精选了3对特异性和敏感性较好、扩增片段分别为228、345和490 bp的引物,并应用所建立的方法对4种分离株的细胞毒和组织毒、田间样品及临床症状相似的疾病猪瘟和猪伪狂犬病毒做了检测;并对田间及实验室样品进行符合率试验,对阳性样品进行重复性试验.与常规RT-PCR法进行比较,证明该方法比常规RT-PCR法敏感性提高了10倍;而且利用该方法可使检测时间大大缩短;特异性试验证实该方法无交叉反应;阳性和阴性样本的符合率均为100％;3次阳性样品重复性检测结果完全相同;稳定性试验显示该试剂盒至少可保存1年以上.本研究所建立的PRRSV一步多重RT-PCR检测方法的敏感性高、特异性强、操作简便、省时、结果重复性好,不仅适用于临床病例尤其低微含量样品的诊断,而且在筛查隐形带毒动物及兽医检疫方面具有重要的应用价值.     

Evaluation of four warming procedures to minimise heat loss induced by anaesthesia and surgery in dogs

OBJECTIVE: To evaluate the efficiency of four warming procedures, introduced after anaesthetic induction and continued during surgery, in minimising heat loss in anaesthetised dogs. DESIGN: Dogs were paired. One of each pair was a control; the other was subjected to one of four warming procedures. METHODS: Ninety-six dogs were involved in total. Pairs of dogs were matched for breed, hair length, and type of surgical procedure and placed adjacent to each other in a large temperature-controlled surgical theatre. One dog within each pair was assigned to one of four warming procedures that commenced immediately after anaesthetic induction. Group 1 (11 pairs) were placed on a purpose-designed prewarmed (41 degrees C) electrically heated pad. Group 2 (18 pairs) were placed on a prewarmed electric heat pad (41 degrees C), cocooned by four wrapped water bottles (initially 41 degrees C) and subjected to radiant heat (150 watt lamp placed 50 cm away from the head of the dog). Group 3 (11 pairs) were surrounded by a forced air warming mattress (set at 43 degrees C). Group 4 (8 pairs) were connected via the anaesthetic breathing circuit to a heater/humidifier (set at 41 degrees C). Rectal temperature measurements were recorded every 15 min for the first 3 h of anaesthesia. The fall in rectal temperature of the control dog was subtracted from the fall in temperature of the treatment dog and this measurement was used to assess the efficacy of the various warming procedures. RESULTS: The mean rectal temperature of unheated 'control' dogs decreased 1.9 +/- 0.6, 1.4 +/- 0.4 and 1.1 +/- 0.4 degrees C over the first, second and third hour respectively. After 3 h the temperature fall differential for all groups were 0.7 +/- 0.7 (Group 1), 3.1 +/- 1.1 (Group 2), 2.4 +/- 1.1 (Group 3) and 1.0 +/- 1.1 degrees C (Group 4). Thus the group 2 procedure was the most successful in preventing a drop of temperature followed by groups 3, 4 and 1. CONCLUSION: Large dogs undergo significant reduction in core body temperature especially during the first 2 h of anaesthesia and surgery. Supplementary warming substantially reduces this fall in body temperature, although certain warming procedures were found to be more effective than others.     

海雀稗SRAP-PCR分子标记体系优化及引物筛选

以4个代表性海雀稗（Paspalum vaginatum）种质的叶片DNA为模板,采用L₉（3⁴）正交试验设计,对影响海雀稗SRAP-PCR反应的Mg²⁺、dNTP、引物和TaqDNA聚合酶的用量进行了优化,并比较了不同浓度模板DNA对扩增的影响,以确定适合海雀稗的SRAP-PCR最佳反应体系。结果表明：海雀稗SRAP-PCR最佳反应体系为10×PCR buffer 1 μL、模板DNA50 ng、Mg²⁺2.5 mmol·L^-1、dNTP150 μmol·L^-1、引物0.4 μmol·L^-1、TaqDNA聚合酶1.0 U,总体积10 μL。利用该反应体系从100对引物中筛选出可扩增清晰条带的引物83对,在83对引物中选出多态性丰富的引物44对。SRAP-PCR反应体系的优化及引物筛选,为今后利用SRAP标记技术进行海雀稗遗传多样性研究、基因定位、遗传图谱的构建以及分子标记辅助育种提供技术支持。