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1.
The self-heating correction is known to modify open-path eddy covariance estimates of net ecosystem CO2 exchange, typically towards reduced uptake or enhanced emissions, but with a magnitude heretofore not generally documented. We assess the magnitude of this correction to be of order 1 μmol m−2 s−1 (daytime) for half-hourly fluxes and consistently over 100 g C m−2 for annual integrations, across a tower network (CARBORED-ES) spanning climate zones from Mediterranean temperate to cool alpine. We furthermore examine the sensitivity of the correction to its determining factors. Due to significant diurnal variation, the means of discriminating day versus night can lead to differences of up to several tens of g C m−2 year−1. Since its principal determinants - temperature and wind speed - do not include gas flux data, the annual correction can be estimated using only meteorological data so as to avoid uncertainties introduced when filling gaps in flux data. For fast retro-correction of annual integrations published prior to the recognition of this instrument surface heating effect, the annual impact can be roughly approximated to within 12 g C m−2 year−1 by a linear function of mean annual temperature. These determinations highlight the need for the flux community to reach a consensus regarding the need for and the specific form of this correction.  相似文献   

2.
The effects of elevated CO2 supply on N2O and CH4 fluxes and biomass production of Phleum pratense were studied in a greenhouse experiment. Three sets of 12 farmed peat soil mesocosms (10 cm dia, 47 cm long) sown with P. pratense and equally distributed in four thermo-controlled greenhouses were fertilised with a commercial fertiliser in order to add 2, 6 or 10 g N m−2. In two of the greenhouses, CO2 concentration was kept at atmospheric concentration (360 μmol mol−1) and in the other two at doubled concentration (720 μmol mol−1). Soil temperature was kept at 15 °C and air temperature at 20 °C. Natural lighting was supported by artificial light and deionized water was used to regulate soil moisture. Forage was harvested and the plants fertilised three times during the basic experiment, followed by an extra fertilisations and harvests. At the end of the experiment CH4 production and CH4 oxidation potentials were determined; roots were collected and the biomass was determined. From the three first harvests the amount of total N in the aboveground biomass was determined. N2O and CH4 exchange was monitored using a closed chamber technique and a gas chromatograph. The highest N2O fluxes (on average, 255 μg N2O m−2 h−1 during period IV) occurred just after fertilisation at high water contents, and especially at the beginning of the growing season (on average, 490 μg N2O m−2 h−1 during period I) when the competition of vegetation for N was low. CH4 fluxes were negligible throughout the experiment, and for all treatments the production and oxidation potentials of CH4 were inconsequential. Especially at the highest rates of fertilisation, the elevated supply of CO2 increased above- and below-ground biomass production, but both at the highest and lowest rates of fertilisation, decreased the total amount of N in the aboveground dry biomass. N2O fluxes tended to be higher under doubled CO2 concentrations, indicating that increasing atmospheric CO2 concentration may affect N and C dynamics in farmed peat soil.  相似文献   

3.
Methane fluxes were measured monthly over a year from tropical peatland of Sarawak, Malaysia using a closed-chamber technique. The CH4 fluxes in forest ecosystem ranged from −4.53 to 8.40 μg C m−2 h−1, in the oil palm ecosystem from −32.78 to 4.17 μg C m−2 h−1 and in the sago ecosystem from −7.44 to 102.06 μg C m−2 h−1. A regression tree approach showed that CH4 fluxes in each ecosystem were related to different underlying environmental factors. They were relative humidity for forest and water table for both sago and oil palm ecosystems. On an annual basis, both forest and sago were CH4 source with an emission of 18.34 mg C m−2 yr−1 for forest and 180 mg C m−2 yr−1 for sago. Only oil palm ecosystem was a CH4 sink with an uptake rate of −15.14 mg C m−2 yr−1. These results suggest that different dominant underlying environmental factors among the studied ecosystems affected the exchange of CH4 between tropical peatland and the atmosphere.  相似文献   

4.
Soil CO2 efflux is a large component of total respiration in many ecosystems. It is important to understand the environmental controls on soil CO2 efflux, in order to evaluate potential responses of ecosystems to climate change. This study investigated the relationship between total soil CO2 efflux and soil temperature, soil moisture and solar radiation on an interannual basis for a plot of temperate deciduous ancient semi-natural woodland at Wytham Woods in central southern England. We also aimed to quantify the contribution of soil organic matter decomposition (SOM), root-and-rhizosphere respiration, and mycorrhizal respiration components to total soil CO2 efflux, and determine their environmental correlates. Total soil CO2 efflux was measured regularly from April 2006 to December 2008 and found to average 4.1 Mg C ha−1 yr−1 in both 2007 and 2008. In addition, we applied a recently developed approach to partition the efflux into SOM, root-and-rhizosphere, and mycorrhizal components in situ using mesh bags. SOM decomposition, root-and-rhizosphere, and mycorrhizal respiration were estimated to contribute 70 ± 6%, 22 ± 6% and 8 ± 3% of total soil CO2 efflux respectively, equating to 3.0 ± 0.3, 0.9 ± 0.2 and 0.3 ± 0.1 Mg C ha−1 yr−1. In order to avoid the effect of temporal correlation between variables caused by seasonality, we investigated interannual variability by examining the relationship between CO2 flux anomalies and anomalies in environmental variables. Variation in soil temperature explained 50% of the interannual variance in soil CO2 efflux, and soil moisture a further 18% of the residual variance. Solar radiation, as a proxy for plant photosynthesis, had no significant effect on total soil CO2 efflux, but was positively correlated with root-and-rhizosphere respiration, and mycorrhizal respiration. The relationship between anomalies in soil CO2 efflux and soil temperature was highly significant, with a sensitivity of 0.164 ± 0.023 μmol CO2 m−2 s−1 °C−1. For mean peak summer efflux rates (2.03 μmol CO2 m2 s−1), this is equivalent to 8% per °C, or a Q10 temperature sensitivity of 2.2 ± 0.2. We demonstrate the utility of an anomaly analysis approach and conclude that soil temperature is the key driver of total soil CO2 efflux primarily through its positive relationship with SOM-decomposition rate.  相似文献   

5.
In boreal forests, canopy-scale emissions of biogenic volatile organic compounds (BVOCs) are rather well characterised, but knowledge of ecosystem-scale BVOC emissions is still inadequate. We used adsorbent tubes to measure BVOCs from a boreal Scots pine (Pinus sylvestris L.) forest floor in southern Finland and analysed the compounds with a gas chromatograph-mass spectrometer. The most abundant compound group was the monoterpenes (averaging 5.04 μg m−2 h−1), in which α-pinene, Δ3-carene and camphene contributed over 90% of the emissions. Emissions of other terpenoids (isoprene and sesquiterpenes) were low (averaging 0.05 and 0.04 μg m−2 h−1, respectively). BVOC emissions from the forest floor varied seasonally, peaking in early summer and autumn, with most of the compounds following similar patterns. The emission pattern was sustained throughout the measurement period, suggesting that the main sources of the emissions remained more or less stable. We compared the BVOC fluxes with environmental parameters such as temperature, precipitation and PAR, and with fluxes of other trace gases (CO2, CH4, N2O), as well as with ground vegetation photosynthesis and with litter input. Several of these parameters were correlated with the presence of BVOCs. The sources of soil BVOC emissions are very poorly understood, but our results suggest, that changes in litter quantity and quality, soil microbial activity and the physiological stages of plants are linked with changes in BVOC fluxes.  相似文献   

6.
The extent to which complex interrelationships between plants and microorganisms influence organic matter dynamics is critical to our understanding of global C cycles in changing environments. We examined the hypothesis that patterns of soil microbial activity and functional composition differ among vegetation types in northern peatland ecosystems. Microbial characteristics were compared among peatlands differing in plant growth form (tree, shrub/moss, sedge) in two regions (New York State and West Virginia). Microbial activity (basal respiration) was greater in surface (0-15 cm) than subsurface (15-30 cm) peat and from sites dominated by shrubs and Sphagnum moss (3.9±0.65 μg C g−1 h−1) compared to forested (1.8±0.20 μg C g−1 h−1) or sedge-dominated sites (1.9±0.38 μg C g−1 h−1). Microbial activity was not related to decomposability of peat organic matter among vegetation types, and activity was unexpectedly higher in sites with lower peat pH and higher water table level. Substrate-induced respiration (SIR) did not show a clear pattern among vegetation types, but was greater in surface than subsurface peat. Microbial responsiveness to added glucose was very low. The ratio of basal respiration to SIR varied between 0.39 and 0.72 and, like activity, was highest in shrub/Sphagnum sites. Microbial substrate utilization patterns (assayed with BIOLOG® GN plates) also differed between shrub/Sphagnum sites and forest or sedge sites, suggesting that C fluxes were mediated by different assemblages of microorganisms in shrub/Sphagnum peatlands. Principal component (PC) scores indicated more utilization of N-containing compounds and carboxylic acids, and less utilization of carbohydrates by microbial communities in shrub/Sphagnum sites. PC scores were much more variable both within and among vegetation types for sites in West Virginia than in New York State, and a greater diversity of C sources were utilized in WV (57±3) than NYS (47±2) peat. Our results suggest a link between microbial respiratory activity and microbial functional composition as they vary among these peatland vegetation types.  相似文献   

7.
Long-term fluxes of sulphur dioxide (SO2) have been measured over a mixed suburban forest subjected to elevated SO2 concentrations. The net exchange was shown to be highly dynamic with substantial periods of both upward and downward fluxes observed in excellent conditions for flux measurement. Upward fluxes constituted 30% of selected fluxes and appeared more frequently when the canopy was acidic. Upward fluxes were shown to be due to desorption from a drying surface or when ambient levels declined after periods of increased SO2 exposure.The long term average SO2 flux (F) was −59 ng SO2 m−2 s−1 for the period 1997-2009 corresponding to an average SO2 concentration of 12.3 μg SO2 m−3 and a deposition velocity υd of 5 mm s−1. The smallest deposition fluxes and υd were measured in dry conditions (−42 ng m−2 s−1 and 3.5 mm s−1, resp.), which represented 57% of all cases. Wet canopies were more efficient sinks for SO2 and a dew-wetted canopy had a smaller υd (6 mm s−1) than a rain-wetted canopy (ca 10 mm s−1). Seasonal variability reflected differences in chemical climate or canopy buffering properties. During the summer half-year when surface acidity was low due to higher NH3/SO2 ratios, a higher deposition efficiency (υd/υdmax) and lower non-stomatal resistance (Rw) were observed compared to winter conditions. Comparisons of Rc for different combinations of canopy wetness and surface acidity categories emphasized the importance of both factors in regulating the non-stomatal sinks of SO2. Increased surface water acidity gradually led to a lower υd/υdmax and an increased Rc for all considered canopy wetness categories. The smallest υd/υdmax ratio and highest Rc were obtained for a dry canopy with high surface acidity. Conversely, a rain-wetted canopy was the most efficient sink for SO2. The canopy sink strength was further enhanced by high friction velocities (u*), optimizing the mechanical mixing into the canopy. Long-term trends were strongly coupled to changes in the NH3/SO2 ratio, which has clearly enhanced the deposition efficiency of SO2 in recent years.  相似文献   

8.
Manure application to managed grassland is a common agricultural practice. There are, however, limited studies looking at the fluxes and interactions of reactive N compounds and aerosols following fertilisation with manure. In this study, state-of-the-art chemical analysers (GRAEGOR, QCLAS, PTRMS) were used to investigate concentrations, fluxes and chemical interactions of reactive nitrogen containing trace gases (NH3, HNO3, HONO) and aerosols (NO3) above a grassland fertilised with 164 kg N ha−1 of cattle slurry. Emissions of NH3 peaked at >67 μg m−2 s−1, based on a 30 min average. The estimated overall loss of total ammoniacal nitrogen (TAN) from the applied slurry through NH3 emissions in the first 5 days was 33.5%. The average trimethylamine flux in the first 31 h following the first slurry application was 40 ng m−2 s−1 and amounted to 0.38% of the NH3-N emissions. Apparent nitrate aerosol emissions were observed following the slurry application peaking at 13.0 ng m−2 s−1. This suggests formation of NH4NO3 from reaction of the emitted NH3 with atmospheric HNO3, consistent with the observation of gaseous concentration products exceeding the dissociation constants of ammonium nitrate. Fluxes of total nitrate (HNO3 + NO3) were bi-directional and positive during the mid-day period after fertilisation, suggesting that the slurry acted as a net source for these compounds. There is evidence of small HONO emission following fertilisation (up to 1 ng m−2 s−1), although the production process is currently not identified. By contrast, all compounds showed deposition to the adjacent unfertilised grassland.  相似文献   

9.
Assessments of terrestrial carbon fluxes require a thorough understanding of links between primary production, soil respiration and carbon loss through drainage. In this study, stem girdling was used to terminate autotrophic soil respiration including rhizosphere respiration and root exudation in a temperate Norway spruce stand. Rates of soil respiration and dissolved organic carbon (DOC) formation were measured in the second year after girdling, comparing an intact plant-rhizosphere continuum with an exclusive decomposer system. The molecular and isotopic composition of DOC in the soil solution was analysed with a coupled Py-GC/MS-C-IRMS system to distinguish between the carbon sources of dissolved carbon. Pyrolysis products were grouped according to their precursor origins: polysaccharides, proteins or of mixed origin (mainly derivates of lignins and proteins). When dead roots became available for decomposition, rates of heterotrophic soil respiration in girdling plots peaked at 6.5 μmol m−2 s−1, comparable to peak rates of total soil respiration (autotrophic and heterotrophic) in control plots, 6.1 μmol m−2 s−1. A significant response of soil respiration to temperature was found in control plots only, showing that an unlimiting supply of organic substrates for microbial respiration may mask any temperature effects. The enhanced decomposition in girdled plots was further supported by the isotopic composition of DOC in soil solution; all three precursor groups became isotopically enriched as the growing season progressed (polysaccharides by 2.3‰, proteins by 1.9‰, mixed origin group by 2.2‰). This indicates a trophic level shift due to incorporation of organic substrate into the microbial food chain. In the control plots’ mixed origin fraction, the isotopic composition changed over time from a signature resembling that of lignin (−28.9‰) to one similar of the protein fraction (−25.7‰). Significant temporal changes of structural DOC composition occurred in the girdling plots only. These results suggest that changes in the microbial community and in decomposition rates occurred in both girdled and control plots in the following ways: (i) increased substrate availability (dead roots) gave rise to generally enhanced performance of the decomposer community in girdled plots, (ii) root-derived exudates probably contributed to enhanced decomposition of recalcitrant lignin in the control plots and (iii) the structural composition of DOC seemed to be more a result of decomposition than of plant root exudation in all plots.  相似文献   

10.
Forest soils contain the largest carbon stock of all terrestrial biomes and are probably the most important source of carbon dioxide (CO2) to atmosphere. Soil CO2 fluxes from 54 to 72-year-old monospecific stands in Rwanda were quantified from March 2006 to December 2007. The influences of soil temperature, soil water content, soil carbon (C) and nitrogen (N) stocks, soil pH, and stand characteristics on soil CO2 flux were investigated. The mean annual soil CO2 flux was highest under Eucalyptus saligna (3.92 μmol m−2 s−1) and lowest under Entandrophragma excelsum (3.13 μmol m−2 s−1). The seasonal variation in soil CO2 flux from all stands followed the same trend and was highest in rainy seasons and lowest in dry seasons. Soil CO2 flux was mainly correlated to soil water content (R2 = 0.36-0.77), stand age (R2 = 0.45), soil C stock (R2 = 0.33), basal area (R2 = 0.21), and soil temperature (R2 = 0.06-0.17). The results contribute to the understanding of factors that influence soil CO2 flux in monocultural plantations grown under the same microclimatic and soil conditions. The results can be used to construct models that predict soil CO2 emissions in the tropics.  相似文献   

11.
Quantifying the nitrous oxide (N2O) and nitric oxide (NO) fluxes emitted from croplands remains a major challenge. Field measurements in different climates, soil and agricultural conditions are still scarce and emissions are generally assessed from a small number of measurements. In this study, we report continuously measured N2O and NO fluxes with a high temporal resolution over a 2-year crop sequence of barley and maize in northern France. Measurements were carried out using 6 automatic chambers at a rate of 16 mean flux measurements per day. Additional laboratory measurements on soil cores were conducted to study the response of NO and N2O emissions to environmental conditions.The detection limit of the chamber setup was found to be 3 ng N m−2 s−1 for N2O and 0.1 ng N m−2 s−1 for NO. Nitrous oxide fluxes were higher than the threshold 37% of the time, while they were 72% of the time for NO fluxes.The cumulated annual NO and N2O emissions were 1.7 kg N2O-N ha−1 and 0.5 kg NO-N ha−1 in 2007, but 2.9 kg N2O-N ha−1 and 0.7 kg NO-N ha−1 in 2008. These inter-annual differences were largely related to crop types and to their respective management practices. The forms, amounts and timing of nitrogen applications and the mineralization of organic matter by incorporation of crop residues were found to be the main factor controlling the emissions peaks. The inter-annual variability was also due to different weather conditions encountered in 2007 and 2008. In 2007, the fractioned N inputs applied on barley (54 kg ha−1 in March and in April) did not generate N2O emissions peaks because of the low rainfall during the spring. However, the significant rainfall observed in the summer and fall of 2007, promoted rapid decomposition of barley residues which caused high levels of N2O emissions. In 2008, the application of dairy cattle slurry and mineral fertilizer before the emergence of maize (107 kg Nmin ha−1 or 130 kg Ntot ha−1 in all) coincided with large rainfalls promoting both NO and N2O emissions, which remained high until early summer.Laboratory measurements corroborated the field observations: NO fluxes were maximum at a water-filled pore space (WFPS) of around 27% while N2O fluxes were optimal at 68% WFPS, with a maximum potentially 14 times larger than for NO.  相似文献   

12.
To assess the impacts of yak excreta patches on greenhouse gas (GHG) fluxes in the alpine meadow of the Qinghai-Tibetan plateau, methane (CH4), carbon dioxide (CO2), and nitrous oxide (N2O) fluxes were measured for the first time from experimental excreta patches placed on the meadow during the summer grazing seasons in 2005 and 2006. Dung patches were CH4 sources (average 586 μg m−2 h−1 in 2005 and 199 μg m−2 h−1 in 2006) during the investigation period of two years, while urine patches (average −31 μg m−2 h−1 in 2005 and −33 μg m−2 h−1 in 2006) and control plots (average −28 μg m−2 h−1 in 2005 and −30 μg m−2 h−1 in 2006) consumed CH4. The cumulative CO2 emission for dung patches was about 36-50% higher than control plots during the experimental period in 2005 and 2006. The cumulative N2O emissions for both urine and dung patches were 2.1-3.7 and 1.8-3.5 times greater than control plots in 2005 and 2006, respectively. Soil water-filled pore space (WFPS) explained 35% and 36% of CH4 flux variation for urine patches and control plots, respectively. Soil temperature explained 40-75% of temporal variation of CO2 emissions for all treatments. Temporal N2O flux variation in urine patches (34%), dung patches (48%), and control (56%) plots was mainly driven by the simultaneous effect of soil temperature and WFPS. Although yak excreta patches significantly affected GHG fluxes, their contributions to the whole grazing alpine meadow in terms of CO2 equivalents are limited under the moderate grazing intensity (1.45 yak ha−1). However, the contributions of excreta patches to N2O emissions are not negligible when estimating N2O emissions in the grazing meadow. In this study, the N2O emission factor of yak excreta patches varied with year (about 0.9-1.0%, and 0.1-0.2% in 2005 and 2006, respectively), which was lower than IPCC default value of 2%.  相似文献   

13.
Savanna landscapes across north Australia are characterised by limited topographic variation, and in the Northern Territory, by a relatively constant decline in rainfall with distance inland. The North Australian Tropical Transect (NATT) traverses this 1000 km gradient of largely intact vegetation which provides an ideal ‘living laboratory’ and framework to investigate the influence of vegetation structural and floristic change and climate drivers on land-atmosphere exchange at a regional scale. We conducted a multidisciplinary program examining carbon, water and energy fluxes as a function of climate and vegetation change along a sub-continental environmental gradient. Initial findings are reported in this Special Issue. During the program, an intensive field campaign was undertaken during the dry season to characterise vegetation and soil properties of eight flux tower sites used to describe spatial and temporal dynamics of fluxes across this gradient. This paper provides an overview of the savanna landscapes of north Australia detailing vegetation structural and physiological change along this gradient. Above-ground woody biomass, stem density, overstorey LAI and canopy height declined along sites that spanned an 1100 mm annual rainfall gradient. Biomass ranged from 35 to 5 t C ha−1 with dry season LAI ranging from ∼1 to 0.05 across savanna sites both intact and cleared for grazing. Across open-forest and woodland savanna, basal area ranged from 9.7 to 5.3 m2 ha−1. While structural change was significant and correlated with rainfall, leaf scale physiological properties (maximal photosynthesis, Vcmax, ci/ca, light use efficiency) of the dominant woody species showed little variation, despite the significant environmental gradient. It is likely that changes in structural properties dominate spatial patterns of flux as opposed to physiological plasticity or species differences along this gradient.  相似文献   

14.
Climate models predict drier conditions in the next decades in the Mediterranean basin. Given the importance of soil CO2 efflux in the global carbon balance and the important role of soil monoterpene and volatile organic compounds (VOCs) in soil ecology, we aimed to study the effects of the predicted drought on soil CO2, monoterpenes and other VOC exchange rates and their seasonal and interannual variations. We decreased soil water availability in a Mediterranean holm oak forest soil by means of an experimental drought system performed since 1999 to the present. Measurements of soil gas exchange were carried out with IRGA, GC and PTR-MS techniques during two annual campaigns of contrasting precipitation. Soil respiration was twice higher the wet year than the dry year (2.27±0.26 and 1.05±0.15, respectively), and varied seasonally from 3.76±0.85 μmol m−2 s−1 in spring, to 0.13±0.01 μmol m−2 s−1 in summer. These results highlight the strong interannual and interseasonal variation in CO2 efflux in Mediterranean ecosystems. The drought treatment produced a significant soil respiration reduction in drought plots in the wet sampling period. This reduction was even higher in wet springs (43% average reduction). These results show (1) that soil moisture is the main factor driving seasonal and interannual variations in soil respiration and (2) that the response of soil respiration to increased temperature is constrained by soil moisture. The results also show an additional control of soil CO2 efflux by physiology and phenology of trees and animals. Soil monoterpene exchange rates ranged from −0.01 to 0.004 nmol m−2 s−1, thus the contribution of this Mediterranean holm oak forest soil to the total monoterpenes atmospheric budget seems to be very low. Responses of individual monoterpenes and VOCs to the drought treatment were different depending on the compound. This suggests that the effect of soil moisture reduction in the monoterpenes and VOC exchange rates seems to be dependent on monoterpene and VOC type. In general, soil monoterpene and other VOC exchange rates were not correlated with soil CO2 efflux. In all cases, only a low proportion of variance was explained by the soil moisture changes, since almost all VOCs increased their emission rates in summer 2005, probably due to the effect of high soil temperature. Results indicate thus that physical and biological processes in soil are controlling soil VOC exchange but further research is needed on how these factors interact to produce the observed VOCs exchange responses.  相似文献   

15.
We measured soil profile concentrations and emission of CO2, CH4 and N2O from soils along a lakeshore in Garwood Valley, Antarctica, to assess the extent and biogeochemical significance of biogenic gas emission to C and N cycling processes. Simultaneous emission of all three gases from the same site indicated that aerobic and anaerobic processes occurred in different layers or different parts of each soil profile. The day and location of high gas concentrations in the soil profile corresponded to those having high gas emission, but the pattern of concentration with depth in the soil profile was not consistent across sites. That the highest gas concentrations were not always in the deepest soil layer suggests either limited production or gas diffusion in the deeper layers. Emission of CO2 was as high as 47 μmol m−2 min−1 and was strongly related to soil temperature. Soil respiration differed significantly according to location on the lakeshore, suggesting that factors other than environmental variables, such as the amount and availability of O2 and nutrients, play an important role in C mineralization processes in these soils. High surface emission (maximum: 15 μmol m−2 min−1) and profile gas concentration (maximum: 5780 μL L−1) of CH4 were at levels comparable to those in resource-rich temperate ecosystems, indicating an active indigenous population of methanogenic organisms. Emission of N2O was low and highly variable, but the presence of this gas and NO3 in some of the soils suggest that denitrification and nitrification occur there. No significant relationships between N2O emission and environmental variables were found. It appears that considerable C and N turnover occurs in the lakeshore soils, and accurate accounting will require measurements of aerobic and anaerobic mineralization. The production and emission of biogenic gases confirm the importance of these soils as hotspots of biological activity in the dry valleys and probable reservoirs of biological diversity.  相似文献   

16.
Phosphomonoesterase (PMEase) activity plays a key role in nutrient cycling and is a potential indicator of soil condition and ecosystem stress. We compared para-nitrophenyl phosphate (pNPP) and 4-methylumbelliferyl phosphate (MUP) as substrate analogues for PMEase in 7 natural ecosystem soils and 8 agricultural top soils with contrasting C contents (8.0-414 g kg−1 C) and pH (3.0-7.5). PMEase activities obtained with pNPP (0.05-5 μmol g−1 h−1) were significantly less than activities obtained with MUP (0.9-13 μmol g−1 h−1), especially in soils with a high organic matter content (>130 g kg−1). Only PMEase activities assayed with MUP correlated significantly with total C and total N (r=0.7, P<0.01 all), and pH (r=−0.71, P<0.01). PMEase activities obtained with the two substrate analogues were correlated when expressed on a C-content basis (r=0.8, P<0.001), but not when expressed on an oven-dry soil weight basis. This indicated that interference by organic matter is related to the quantity rather than to the quality of organic matter. Overall, assaying with MUP was more sensitive compared to assaying with pNPP, particularly in the case of high organic and acid soils.  相似文献   

17.
Like other N-fixing invasive species in Hawaii, Falcataria moluccana dramatically alters forest structure, litterfall quality and quantity, and nutrient dynamics. We hypothesized that these biogeochemical changes would also affect the soil microbial community and the extracellular enzymes responsible for carbon and nutrient mineralization. Across three sites differing in substrate texture and age (50-300 years old), we measured soil enzyme activities and microbial community parameters in native-dominated and Falcataria-invaded plots. Falcataria invasion increased acid phosphatase (AP) activities to >90 μmol g−1 soil h−1 compared to 30-60 μmol g−1 soil h−1 in native-dominated stands. Extracellular enzymes that mineralize carbon and nitrogen also increased significantly under Falcataria on the younger substrates. By contrast, total microbial biomass and mycorrhizal abundance changed little with invasion or substrate. However, fungal:bacterial ratios declined dramatically with invasion, from 2.69 and 1.35 to <0.89 on the 50- and 200-year-old substrates, respectively. These results suggest that Falcataria invasion alters the composition and function of belowground soil communities in addition to forest structure and biogeochemistry. The increased activities of AP and other enzymes that we observed are consistent with a shift toward phosphorus limitation and rapid microbial processing of litterfall C and N following Falcataria invasion.  相似文献   

18.
We evaluated the spatial structures of nitrous oxide (N2O), carbon dioxide (CO2), and methane (CH4) fluxes in an Acacia mangium plantation stand in Sumatra, Indonesia, in drier (August) and wetter (March) seasons. A 60 × 100-m plot was established in an A. mangium plantation that included different topographical elements of the upper plateau, lower plateau, upper slope and foot slope. The plot was divided into 10 × 10-m grids and gas fluxes and soil properties were measured at 77 grid points at 10-m intervals within the plot. Spatial structures of the gas fluxes and soil properties were identified using geostatistical analyses. Averaged N2O and CO2 fluxes in the wetter season (1.85 mg N m−2 d−1 and 4.29 g C m−2 d−1, respectively) were significantly higher than those in the drier season (0.55 mg N m−2 d−1 and 2.73 g C m−2 d−1, respectively) and averaged CH4 uptake rates in the drier season (−0.62 mg C m−2 d−1) were higher than those in the wetter season (−0.24 mg C m−2 d−1). These values of N2O fluxes in A. mangium soils were higher than those reported for natural forest soils in Sumatra, while CO2 and CH4 fluxes were in the range of fluxes reported for natural forest soils. Seasonal differences in these gas fluxes appears to be controlled by soil water content and substrate availability due to differing precipitation and mineralization of litter between seasons. N2O fluxes had strong spatial dependence with a range of about 18 m in both the drier and wetter seasons. Topography was associated with the N2O fluxes in the wetter season with higher and lower fluxes on the foot slope and on the upper plateau, respectively, via controlling the anaerobic-aerobic conditions in the soils. In the drier season, however, we could not find obvious topographic influences on the spatial patterns of N2O fluxes and they may have depended on litter amount distribution. CO2 fluxes had no spatial dependence in both seasons, but the topographic influence was significant in the drier season with lowest fluxes on the foot slope, while there was no significant difference between topographic positions in the wetter season. The distributions of litter amount and soil organic matter were possibly associated with CO2 fluxes through their effects on microbial activities and fine root distribution in this A. mangium plantation.  相似文献   

19.
There is now clear evidence for a prolonged increase in atmospheric CO2 concentrations and enrichment of the biosphere with N. Understanding the fate of C in the plant-soil system under different CO2 and N regimes is therefore of considerable importance in predicting the environmental effects of climate change and in predicting the sustainability of ecosystems. Swards of Lolium perenne were grown from seed in a Eutric Cambisol at either ambient (ca. 350 μmol mol−1) or elevated (700 μmol mol−1) atmospheric pCO2 and subjected to two inorganic N fertilizer regimes (no added N and 70 kg N ha−1 month−1). After germination, soil solution concentrations of dissolved organic C (DOC), dissolved inorganic N (DIN), dissolved organic N (DON), phenolics and H+ were measured at five depths down the soil profile over 3 months. The exploration of soil layers down the soil profile by roots caused transient increases in soil solution DOC, DON and phenolic concentrations, which then subsequently returned to lower quasi-stable concentrations. In general, the addition of N tended to increase DOC and DON concentrations while exposure to elevated pCO2 had the opposite effect. These treatment effects, however, gradually diminished over the duration of the experiment from the top of the soil profile downwards. The ambient pCO2 plus added N regime was the only treatment to maintain a notable difference in soil solution solute concentration, relative to other treatments. This effect on soil solution chemistry appeared to be largely indirect resulting from increased plant growth and a decrease in soil moisture content. Our results show that although plant growth responses to elevated pCO2 are critically dependent upon N availability, the organic chemistry of the soil solution is relatively insensitive to changes in plant growth once the plants have become established.  相似文献   

20.
Eleven species of common fungi from compost were analysed for their content of ergosterol and phospholipid fatty acids (PLFAs) after growth on agar media. Mean content of ergosterol was 3.1 mg g−1 dw of fungal mycelium (range 1-24 mg g−1 dw). Total amount of PLFAs varied between 2.6 and 43.5 μmol g−1 dw of fungi (mean 14.9 μmol g−1 dw). The most common PLFAs were 16:0, 18:2ω6,9 and 18:1ω9, comprising between 79 and 97 mol% of the total amount of PLFAs. The PLFA 18:2ω6,9, suggested as a marker molecule for fungi, comprised between 36 and 61 mol% of the total PLFAs in the Ascomycetes, between 45 and 57 mol% in the Basidiomycetes and 12-22 mol% in the Zygomycetes. There was a good correlation between the content of the two fungal marker molecules, ergosterol and the PLFA 18:2ω6,9, with a mean content of 1 mg ergosterol being equivalent to 2.1 μmol of 18:2ω6,9. Based on results from the fungal isolates, conversion factors were calculated (5.4 mg ergosterol g−1 biomass C and 11.8 μmol 18:2ω6,9 g−1 biomass C) and applied to compost samples in which both the ergosterol and the PLFA 18:2ω6,9 content had been measured. This resulted in similar estimates of fungal biomass C using the two marker molecules, but was three to five times higher than total microbial biomass C calculated using ATP content in the compost. This could partly be explained by the fact that both of the markers used for fungal biomass are cell membrane constituents. Thus, the ergosterol and the PLFA content were related to the hyphal diameter of the fungi, where fungi with thinner hyphae had higher ergosterol concentrations than fungi with thicker hyphae. This could also partly explain the large interspecific variation in content of the two marker molecules.  相似文献   

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