Wood‐rotting basidiomycetes are a minor component of fungal communities associated with Acacia hybrid trees grown for sawlogs in South Vietnam

Acacia hybrid (Acacia mangium × Acacia auriculiformis) clones are widely planted in Vietnam with a total of approximately 400,000 ha to meet the demand for pulpwood, sawn timber and wood chip exports. Silvicultural techniques such as pruning and thinning have been applied to improve productivity and sawlog quality of Acacia hybrid plantations. However, those techniques may also create opportunities for wood decay fungi to enter the Acacia hybrid stems through wounds and cause stem defects that reduce sawlog quality and the value of the plantation. The presence of fungal decay agents in Acacia hybrid trees was examined in two Vietnamese plantations. In July 2011, just prior to a second thinning, discoloured wood samples were taken from a three‐year‐old Acacia hybrid plantation at Phan Truong Hai for the isolation of fungi. In July 2012, approximately 18 months after pruning and thinning treatments, discoloured wood samples were taken from a three‐year‐old Acacia hybrid plantation at Nghia Trung for the isolation of fungi. DNA sequencing of the rDNA ITS identified the isolates. In May 2015, approximately 4 years after thinning and fertilizer treatments, discoloured and decayed wood samples were taken from the above (7‐year‐old) Acacia hybrid plantation at Phan Truong Hai for fungal identification. DNA was extracted directly from discoloured and decayed wood samples and fungal rDNA ITS amplicons sequenced on a Roche 454 sequencer. The results showed that silvicultural treatments did not affect the fungal communities associated with discoloured and decayed wood of Acacia hybrid plantation at Phan Truong Hai. A total of 135 fungal species or OTUs (operational taxonomic units) were identified, including 82 members of Ascomycota and 52 Basidiomycota.     

Quantifying stem discoloration and decay following pruning and thinning an Acacia hybrid plantation

Acacia hybrid (Acacia mangium × A. auriculiformis) is widely planted in Vietnam, and part of the estate is managed for solid timber products. This requires pruning and thinning – practices that through mechanical wounding can facilitate the entry of fungal organisms, leading to stem defects. The extent to which this happens in Acacia hybrid has not been previously studied in Vietnam. A destructive survey was conducted in a 3‐year‐old Acacia hybrid plantation at Nghia Trung in Binh Phuoc province, 18 months after the imposition of pruning and thinning treatments. Pruned trees had a higher incidence and severity of discoloration and decay in the stem than unpruned trees; thinning increased the incidence of stem decay and the severity of discoloration but not the severity of decay. An interaction between pruning and thinning did not influence the incidence of stem decay but did increase the severity of discoloration in thinning treatments. Across treatments, levels of discoloration and decay were <30% and <5%, respectively. Modification of current pruning practices may be necessary to ensure that decay levels are kept within acceptable limits at an anticipated harvest age of 7–8 years.     

Growth and wood basic density of acacia hybrid clones at three locations in Vietnam

Field trials testing a total of 27 clones of the interspecific hybrid Acacia mangium × A. auriculiformis and seedling controls of the parental species were established at Ba Vi and Yen Thanh in the north of Vietnam and Long Thanh in the south. At both Ba Vi and Yen Thanh there were significant (P < 0.001) differences in height and diameter at breast height (DBH) among 22 tested clones at 4 years. At Long Thanh, twelve hybrid clones did not differ significantly in DBH at age 3 years, but did (P < 0.001) at age 5 years. At the two northern sites the acacia hybrid clones had significantly greater DBH than control seedlots of the parental species. At Long Thanh, DBH of the hybrid clones and A. mangium was similar, with a genetically improved seedlot of A. mangium displaying the best DBH. Mean wood basic density at breast height of the acacia hybrid clones was 539 kg m⁻³ at Yen Thanh at age 8 years, and 473 kg m⁻³ at Long Thanh at age 5 years; density for A. mangium at Long Thanh was only slightly lower than the hybrid clones at 461 kg m⁻³. Linear regressions of Pilodyn penetration (PP) at breast height on wood basic density explained 60% of the variance in density of treatments (clones and control seedlots) at Yen Thanh and 36% at Long Thanh. There were significant differences between hybrid clones in PP at all three trial sites. Clonal DBH performance was not strongly correlated across the three trial sites; Pearson correlations of clone mean DBH between pairs of sites ranged from −0.47 to 0.20. Clonal rankings for PP were more stable, with Pearson correlations between pairs of sites ranging from r = 0.71 to 0.78.     

Facilitation by two exotic Acacia: Acacia auriculiformis and Acacia mangium as nurse plants in South China

Because of their high growth rate and tolerance to bare soil, two exotic Acacia species, Acacia auriculiformis and Acacia mangium, have been commonly planted in degraded areas of South China. With their large canopies and ability to fix nitrogen, the two Acacia species have also been considered to act as nurse plants for understory plants. The current study clarified the nursing effects of the Acacia species by comparing microclimate characteristics and physiological traits of native plant seedlings at three sites: under the canopies of the each Acacia species and on bare land (open site). Although the sites were not replicated, the results indicated that adult trees of both Acacia species can facilitate native species, but that A. mangium has greater facilitating effects due to greater temperature buffering, radiation reduction, and nutrient amelioration. In response to facilitation, three species (Castanopsis hystrix, Michelia macclurei, and Manglietia glauca) with different shade-tolerant traits growing under Acacia canopies expressed distinct adaptations. For the three species, the chlorophyll fluorescence curves of rETR and ΔF/Fm′ were higher under A. auriculiformis and on the open site than under A. mangium. The maximum quantum yield in PSII(Fv/Fm) in diurnal changes of the three species showed that all the Fv/Fm values were between 0.70 and 0.84 and that the Fv/Fm values were mostly higher under A. mangium than on the open site or under A. auriculiformis. Total chlorophyll content and both chlorophyll a and b contents in the three species were higher under the Acacia species than on the open site, while chlorophyll a/b ratio was higher on the open site. In contrast, the carotenoid content in C. hystrix and M. macclurei was lower under the two Acacia species than on the open site, while the opposite was true for M. glauca. The results demonstrate that the adaptation of the understory species to abiotic environmental factors is not restricted to a single mechanism but apparently involves a group of interrelated, adaptive suites. And also these adaptations were species-specific and especially related to their shade tolerance.     

Polyphenols in Acacia mangium and Acacia auriculiformis heartwood with reference to heart rot susceptibility

The heartwood of Acacia mangium is vulnerable to heart rot and this is the first study to investigate the role of heartwood extractives in its susceptibility. Acacia auriculiformis was compared with A. mangium because it is rarely associated with heart rot. The heartwood extracts of both species were dominated by three flavonoids (2,3-trans-3,4′,7,8-tetrahydroxyflavanone, teracacidin, and 4′,7,8,-trihydroxyflavanone), which were purified and identified by nuclear magnetic resonance spectroscopy. The latter compound has not been previously reported in A. mangium and evidence for melacacidin is also newly reported. The mass spectrometric (MS) behavior of these compounds is given, for example teracacidin does not form molecular ions by either electrospray ionization or atmospheric-pressure chemical ionization. The nature of Acacia tannins was compared to quebracho tannin (composed of profisetinidins) using oxidative cleavage to enable MS detection but a negative reaction was obtained for both, which suggests the Acacia tannins may also be of the 5-deoxy proanthocyanidin type. The concentration of flavanones was less when A. mangium heartwood was decayed but the amount of proanthocyanidins was only slightly reduced and therefore these compounds may be more resistant to degradation by heart rot fungi. We found that the total phenol content of A. auriculiformis was about fivefold that of A. mangium, and, while preliminary, this provides evidence for a role played by phenolic extractives in heart rot resistance of these Acacia species.     

Variation in wood properties of six natural acacia hybrid clones in northern Vietnam

Wood properties of six 8-year-old natural acacia hybrid clones between Acacia mangium and Acacia auriculiformis, planted in Bavi, Vietnam, were studied. The hybrid clones possessed obvious heterosis in growth and in some wood properties. The characteristics of growth, air-dry specific gravity, lengths of fibers and vessel elements, S₂ microfibril angle, green moisture content, and shrinkage were examined to clarify the variation among clones. From the results, the differences among the clones in growth and in some wood properties were significant. The pattern of distribution of specific gravity showed that there were low and high specific gravity zones in the stem. Specific gravity at stump height or at 3.0 m was useful for prediction of specific gravity in the whole tree stem. Moreover, clones with high specific gravity can be predicted at a young age. There was no significant correlation between diameter growth and specific gravity. Of the six clones studied, clone BV5 was selected as the best based on its growth ability and specific gravity. Part of this report was presented at the 57th Annual Meeting of the Japan Wood Research Society, Hiroshima, August 2007     

Possibility of improvement in fundamental properties of wood of acacia hybrids by artificial hybridization

Growth, specific gravity, and wood fiber length of Acacia mangium, Acacia auriculiformis, artificial acacia hybrid clones, and combinations, which were planted in a trial forest in Bavi, Vietnam, in July 2001, were examined. The radial variations from pith to bark were investigated to clarify the effect of genetic factors on these traits. Superiority of hybrids over their parents ranged from 36.3% to 41.6% for diameter, from 20.0% to 25.3% for height, from 6.9% to 20.7% for specific gravity, and from 6.1% to 12.8% for wood fiber length. The hybrid possessed heterosis in diameter, height, specific gravity, and wood fiber length regardless of whether the female parent was A. mangium or A. auriculiformis. The profiles of wood fiber length and specific gravity in the radial direction were similar for all the trees investigated. Wood fiber length was initially 0.5–0.6 mm near the pith and then increased slowly, finally reaching 1.0–1.2 mm near the bark. The specific gravity of acacia increased from 0.49–0.58 near the pith to 0.63–0.74 near the bark. From a relative distance of 30% from the pith, the specific gravity increased slightly and seemed to be stable. The relations among tree diameter, specific gravity, and wood fiber length were fair and could be represented by positive linear regression formulas. Hybrids for which A. auriculiformis was the female parent and A. mangium was the male parent had a faster growth rate and longer wood fibers than the inverse hybrids. Part of this report was presented at the 6th Pacific Regional Wood Anatomy Conference, Kyoto, Japan, December 2005     

Effect of growing site on the fundamental wood properties of natural hybrid clones of <Emphasis Type="Italic">Acacia</Emphasis> in Vietnam

We investigated clonal and site variations in wood fiber length, microfibril angle, and specific gravity of seven natural hybrid clones of Acacia (Acacia mangium × Acacia auriculiformis) grown in northern and southern Vietnam. Fiber length did not differ between clones or between sites. The microfibril angle of the S₂ layer did not significantly differ between clones but significantly differed between sites. Clone and site significantly affected specific gravity. The significant effects of genetic × environmental interactions on wood properties indicated the difference in the response of clones to different growing conditions. The trends of changes in fiber length, microfibril angle, and specific gravity from the vicinity of the pith to near the bark were similar for all clones at each site; however, variations in fiber length, microfibril angle, and specific gravity were more visible in northern Vietnam than in southern Vietnam, with a significant effect of genetic factors. This difference may be attributable to winter, which is experienced in northern Vietnam but not in southern Vietnam. For clone selection for plantation in the northern region, combining growth rate with wood properties was recommended. On the other hand, for plantation in the southern region, clone selection depends mainly on the growth rate, taking into consideration the specific gravity.     

Ecophysiology of Acacia species in wet–dry tropical plantations

Selected tropical Acacia species are used extensively for short-rotation plantation forestry in many parts of Asia and, to a limited degree, in Australia. We explored leaf-level photosynthetic activity and leaf water potential (Ψleaf) of three field-grown Acacia tree species (aged between 7 and 18 months) in contrasting wet–dry tropical plantations in southern Vietnam and northern Australia. Light-saturated photosynthetic rate (A1500) declined throughout the morning and early afternoon in the dry season; in the wet season, levels remained high and relatively constant throughout most of the day. Maximum daily A1500 at 09:00 ranged from 22.2 μmol?m?2?s?1 in the wet to 10.4 μmol?m?2?s?1 in the dry season. At both locations, trees were able to extract soil water such that pre-dawn leaf water potential (Ψpd) remained>?1.5?MPa even at the end of the dry season. Stomatal conductance to water vapour (gs) did not respond to decreasing Ψleaf during the wet season but was sensitive to changes in Ψleaf in the dry season. Species comparisons of the relationships between A1500 and Ψleaf revealed different strategies to balance carbon uptake and water loss in a wet–dry environment. Acacia crassicarpa and A. mangium regulated Ψleaf to a greater extent than the A. mangium×A. auriculiformis hybrid such that ?Ψleaf (determined as Ψpd?midday Ψleaf) was unaffected by season. This result suggests that the hydraulic regulation of tree water status varies amongst young tropical Acacia species. From a management perspective, for Acacia species that tend to strongly regulate water loss in environments with an extended dry season, overall productivity at the end of a rotation may be less than for species that prioritise carbon gain.     

Growth and Development Responses of Three Acacia Species to Long-Term Light Regimes

Abstract

Three species of Acacia which are used in various forestry systems in Micronesia are the Acacia confusa Merr., A. mangium Willd., and A. auriculiformis Cunn. ex Benth. The responses of these species to limited light have not been established. The objective of this study was to determine the growth responses of these three species to light ranging from 19% to 100% sunlight transmission in order to determine the relative tolerance of these species to shade. Several common adaptations to low light occurred with all three species, such as increased leaf surface area in relation to mass of supportive tissue and reduced leaf mass per unit leaf area. However, other adaptations to low light were not evident. Numerous indicators of growth were decreased at low levels of sunlight transmission. The relative reduction in growth at 19% transmission compared with 100% transmission was similar for each species. The results indicate that these Acacia species exhibit some adaptations to development in limited light, but they are not tolerant of shade. All three species are thus classified as shade avoiders, and have limited application under shaded conditions in any Pacific island forestry system.     

Photosynthesis in co-occurring invasive Acacia spp. and native Bornean heath forest trees at the post-establishment invasion stage

Seedlings of invasive species often exhibit superior physiological traits that facilitate their spread at early stages of invasion, although it is unclear whether these traits persist at the post-establishment stage. To determine whether mature exotic Acacia spp. possess superior traits over mature native plants, we compared foliar gas exchange and chlorophyll a fluorescence of Acacia auriculiformis and Acacia mangium coexisting with tropical heath forest tree species Buchanania arborescens and Dillenia suffruticosa in Brunei Darussalam. The CO₂ assimilation rates of Acacia spp. were significantly higher than those of heath species at current prevailing conditions of ~400 ppm ambient CO₂, 1,500 µmol m^?2 s^?1 photosynthetically active radiation and 30°C leaf temperature. The photosystem II of Acacia spp. exhibited significantly higher maximum quantum yield of primary photochemistry at comparable temperatures, and was more sensitive to an elevated temperature (42°C for 1 h). Better photosynthetic performance of Acacia spp., due to larger stomatal openings, better light harvesting efficiency, and greater plasticity in photosystem II, may enable adult Acacia trees to sustain a competitive growth advantage and suppress native tropical heath forest species. The competitive advantage maintained by Acacia spp. in post-establishment stage likely facilitates the establishment of monospecific Acacia stands in invaded heath forests.     

Effect of environment on the response of Eucalyptus clones to inoculation with Cryphonectria cubensis

The ascomycete Cryphonectria cubensis causes severe losses in Eucalyptus plantations in South Africa and selection programmes for disease tolerance are necessary. The aim of this study was to use two C. cubensis inoculation trials, planted at different locations to assess the disease susceptibility of the clones and the effect of the environment on disease development. These two trials consisted each of 21 Eucalyptus clones (E. grandis, E. grandis × camaldulensis and E. grandis × urophylla). All trees were inoculated with a single virulent strain of C. cubensis and lesion widths measured 6 and 12 months after inoculation. Clones differed significantly in their tolerance to C. cubensis. Further, disease severity differed depending on the geographical location of the trial. A significant clone × locality (genotype × environment) interaction was observed. Therefore, screening for disease resistance should take place only in areas where clones will be commercially grown.     

Root activity of young <Emphasis Type="Italic">Acacia mangium</Emphasis> Willd trees: influence of stand density and pruning as studied by <Superscript>32</Superscript>P soil injection technique

Initial spacing and pruning are silvicultural strategies that influence the resource acquisition capabilities of trees. A field study was conducted in the humid tropics of peninsular India to test the assumptions that: (1) high stand density of Acacia mangium induces greater root uptake capacity close to the stem and from the subsoil; and (2) crown pruning stimulates greater root uptake capacity at proximal points. Root activity pattern of two-year-old A. mangium was evaluated as a function of three population densities (1,250, 2,500 and 5,000 stems ha⁻¹), with, and without 50% crown pruning, using ³²P soil injection. The label was placed at 25, 50 and 75 cm lateral distances and at 30 and 60 cm depth. Low density stands (1,250 stems ha⁻¹) generally showed higher ³²P recovery (P < 0.01), which was exaggerated by pruning. Pruned low density stands had 34% root activity at 25 cm, as against 23% for unpruned. The low density stands also showed higher root activity at 75 cm, signifying greater root spread. We suggest that high stem densities favour restricted spread of absorbing roots and may facilitate competitive downward displacement of roots. Pruning the lateral shoots at low stem densities may simulate this to some extent. The net outcome of interactions, however, will depend on trade-offs between stem density and tree management over time.     

First report of 16Sr II‐C subgroup phytoplasma association with Acacia mangium in Tripura,India

Leaf yellowing symptoms were observed on Acacia mangium in the Sipahijala district of Tripura, India, during June 2017. Symptomatic and asymptomatic leaf samples (three of each) were collected from roadside trees of A. mangium for DNA extraction using the CTAB method. Amplicons of ~1.25 kb and ~480 bp were detected in all the symptomatic samples using the phytoplasma‐specific universal 16S rRNA and secA gene primers. Pair wise sequence analysis of 16S rRNA gene sequences, virtual RFLP and phylogenetic analysis revealed that the phytoplasma strain associated with A. mangium belonged to phytoplasma subgroup 16SrII‐C. This is the first report of an association between the 16SrII‐C subgroup and A. mangium leaf yellowing.     

In situ visualization of hardwood microcracks occurring during drying

Microcracks produced in an Acacia hybrid (Acacia mangium × Acacia auriculiformis) and Melia azedarach during drying were visualized in situ using confocal laser scanning microscopy (CLSM); the morphological differences were compared. In the Acacia hybrid, numerous microcracks were found between the wood fiber and ray parenchyma, which propagated toward both the pith and bark. The microcracks closed with further drying, but persisted until the last stage of drying. In Melia azedarach, however, few microcracks formed between the wood fiber and ray parenchyma in the latewood region; they also propagated toward both the pith and bark. Because the microcracks subsequently closed, some could not be detected by CLSM. These morphological characteristics resulted from differences in the wood structure and we conclude that the interface zone between the wood fiber and ray parenchyma is one type of weak point on the transverse surface that is susceptible to checking.     

Genetic diversity of the myrtle rust pathogen (Austropuccinia psidii) in the Americas and Hawaii: Global implications for invasive threat assessments

Since the myrtle rust pathogen (Austropuccinia psidii) was first reported (as Puccinia psidii) in Brazil on guava (Psidium guajava) in 1884, it has been found infecting diverse myrtaceous species. Because A. psidii has recently spread rapidly worldwide with an extensive host range, genetic and genotypic diversities were evaluated within and among A. psidii populations in its putative native range and other areas of myrtle rust emergence in the Americas and Hawaii. Microsatellite markers revealed several unique multilocus genotypes (MLGs), which grouped isolates into nine distinct genetic clusters [C1–C9 comprising C1: from diverse hosts from Costa Rica, Jamaica, Mexico, Puerto Rico, and USA‐Hawaii, and USA‐California; C2: from eucalypts (Eucalyptus spp.) in Brazil/Uruguay and rose apple (Syzygium jambos) in Brazil; C3: from eucalypts in Brazil; C4: from diverse hosts in USA‐Florida; C5: from Java plum (Syzygium cumini) in Brazil; C6: from guava and Brazilian guava (Psidium guineense) in Brazil; C7: from pitanga (Eugenia uniflora) in Brazil; C8: from allspice (Pimenta dioica) in Jamaica and sweet flower (Myrrhinium atropurpureum) in Uruguay; C9: from jabuticaba (Myrciaria cauliflora) in Brazil]. The C1 cluster, which included a single MLG infecting diverse host in many geographic regions, and the closely related C4 cluster are considered as a “Pandemic biotype,” associated with myrtle rust emergence in Central America, the Caribbean, USA‐Florida, USA‐Hawaii, Australia, China‐Hainan, New Caledonia, Indonesia and Colombia. Based on 19 bioclimatic variables and documented occurrences of A. psidii contrasted with reduced sets of specific genetic clusters (subnetworks, considered as biotypes), maximum entropy bioclimatic modelling was used to predict geographic locations with suitable climate for A. psidii which are at risk from invasion. The genetic diversity of A. psidii throughout the Americas and Hawaii demonstrates the importance of recognizing biotypes when assessing the invasive threats posed by A. psidii around the globe.     

Effect of the lateral growth rate on wood properties in fast-growing hardwood species

We investigated the feasibility of using several fast-growing tropical or subtropical hardwood species for timber production by measuring key wood qualities in relationship to the high rates of lateral growth. The trees tested were sampled from even-aged plantations of Acacia mangium, A. auriculiformis, hybrid Acacia (A. mangium × A. auriculiformis), Eucalyptus grandis, E. globulus, and Paraserianthes falcataria (Solomon and Java origin) that had already reached commercial harvesting age. The released strain of the surface growth stress (RS), xylem density (XD), microfibril angle (MFA), and fiber length (FL) were measured at the outermost part of the xylem at breast height in each tree. Results were then compared to the lateral growth rate (radius/age) at breast height, which provides a relative indicator of the amount of tree growth per year. Our findings indicated that RS was constant, regardless of lateral growth rate in each species. Similar results were observed for XD, MFA, and FL, with a few exceptions, suggesting that high growth rates do not intrinsically affect the wood properties of fast-growing tropical or subtropical species that have reached harvesting age. However, special attention must be paid to patterns of xylem maturation when developing plantations of such species.     

Effect of four multipurpose tree species on soil amelioration during tree fallow in Central Togo

The effects of Cassia siamea, Albizia lebbek, Acacia auriculiformis, and Azadirachta indica on soil fertility have been studied on five-year-old fallows on Ferric Acrisols in Central Togo. Litter quality and soil fertility under the four species were significantly different. Topsoil pH increases significantly with increasing litter Ca levels. Cassia siamea and Azadirachta indica were superior in enriching the sandy-loamy topsoils with Calcium and in increasing soil pH. Under Acacia, which had the highest biomass production, litter accumulation appeared to be responsible for the low mineral soil Ca and P values. In addition, topsoil pH under Acacia was lower than under grass or bush fallow or the other species. Slow litter mineralization of Acacia auriculiformis was probably caused by the thick, leathery consistence and high tannin content of its litter. Due to its high biomass production supporting soil acidification pure Acacia auriculiformis stands seemed to be less favourable for improving soil fertility on planted fallows but more suited for firewood plantations and topsoil protection. The foliage as well as the litter and topsoil under Albizia showed narrow C/N- and C/P-ratios resulting in easily mineralizable organic matter. All tree species tested were superior to natural grass/herb fallow in building up surface soil fertility. However, differences with natural bush fallow were not significant.

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Résumé Au bout de cinq ans des differences fortement significative ont été trouvées quant aux caractères de la litière et de la fertilité des jachères arborées avec Cassia siamea, Albizia lebbek, Acacia auriculiformis et Azadirachta indica, respectivement. ll y a une corrélation positive entre le pH de l'horizon superficiel et la teneur en Ca dans la litière: l'enrichissement du sol sable-limoneux en Ca et le pH sont plus élevés sous Cassia siamea et Azadirachta indica que sous les autres espèces. Acacia se caractérise par la plus grande production en biomasse, donc une accumulation importante de litière sur le sol, ce qui entraîne les plus faibles teneurs en P et Ca dans l'horizon de surface. En plus, il semble qu'elle fait diminuer le pH parce que les valeurs trouvées sont inférieures à celles des parcelles témoin ou des autres espèces. La mineralisation retardée de la litière de Acacia auriculiformis depend probablement de la consistence des feuilles et du content élevé de tannine. Acacia est par consequent moins favorable à des jachères plantées mais plutôt efficace quant à la production du bois de feu et la protection du sol contre l'érosion. Vu les petits rapport C/N et C/P dans les feuilles, la litière et dans l'horizon superficiel d'Albizia, on peut supposer que sa matière organique soit plus facilement décomposable. Toutes les espèces d'arbre étudiées sont plus capables de lever la fertilité des sols que les herbes des parcelles témoin. La comparaison avec une jachère spontanée d'arbustes ne fait pas apparâitre d'effets significatifs.

     

Intraspecific response of Pinus taeda L. to Grosmannia huntii and Leptographium terebrantis infection

We examined intraspecific and inter‐year variation in tolerance of Pinus taeda to two ophiostomatoid fungi, Leptographium terebrantis and Grosmannia huntii. Containerized seedlings of P. taeda from 27, 32, 17 and 23 different elite genetic families were artificially inoculated with L. terebrantis and G. huntii in years 2013, 2014, 2016 and 2017, respectively. Six connector families were inoculated every year. Eight weeks post‐inoculation, lesion and occlusion were measured on each seedling to determine the relative susceptibility/tolerance of families to these fungi. Pinus taeda families widely differed in these parameters suggesting intraspecific variation in the susceptibility/tolerance to the inoculated pathogens. The overall tolerance of the connector families to these fungi varied among the experimental years. These results showed that intraspecific variation to L. terebrantis and G. huntii exists among P. taeda families and it could be possible to select tolerant families to minimize the potential impact due to these fungi.     

Application of hot water emasculation to <Emphasis Type="Italic">Acacia auriculiformis</Emphasis> for controlled pollination

To determine the viability of hot water emasculation for Acacia auriculiformis, spikes composed of about 100 flowers were soaked in hot water at varying temperatures (45–60°C) and for varying lengths of time (5 s to 7 min) prior to anthesis. The rate of squashed polyads, each of which consisted of 16 pollen grains, and the germinating rate on media, decreased with temperature and time. A mating test suggested that the rates of adhering polyads per stigma and germinating polyads per stigma were lower in the self-pollination of soaked spikes than in that of normal spikes. On the other hand, the number of matured pods was not significantly different in the mating between the spikes receiving the soaking treatment (♀) and the spikes receiving no treatment (♂) compared with the mating between normal spikes. Although further research is needed to determine the optimal treatment conditions, these results suggest that soaking spikes into hot water, especially at 50°C for 45 s, 54°C for 15 s, and 56°C for 10 s, is an effective emasculation technique.