Effect of deficiencies of single essential amino acids on nitrogen and energy utilisation in chicks.

1. Diets 50% deficient in single essential amino acids were fed to chicks from day 8 to day 18 after hatching to evaluate body-weight gain, food consumption, body composition, nitrogen (N) and energy utilisation. 2. Body-weight gain was reduced most severely by deficiency of isoleucine followed in decreasing order by threonine, arginine, valine, histidine, tryptophan, methionine plus cystine, phenylalanine plus tyrosine, leucine and lysine, and possible reasons for the differences are discussed. 3. Body-weight gain and food efficiency were highly correlated with food consumption but metabolisable energy value of diets was not affected by single essential amino acid deficiencies. 4. Generally N retention (N retained/N consumed) and energy retention (energy retained/energy consumed) reflected food consumption, except for a lower N retention by chicks fed on the methionine plus cystine-deficient diet and for a lower energy retention by chicks fed on the valine deficient diet. 5. The amino acid deficient in the diet was present at very low concentration in the blood plasma.     

Effect of urea on growth,food utilisation and body composition of chicks

1. A study was made of the effect of urea, added to chick diets of different protein contents with or without added methionine, on growth and body composition.

2. In chicks fed on diets containing urea, concentrations of urea were higher in the blood and digestive tract but not in the excreta, as compared with chicks fed on diets containing soyabean as the only source of protein.

3. Urea‐nitrogen was utilised better for growth when the diets were supplemented with methionine rather than unsupplemented.

4. Body fat content was decreased with the increase in protein content of the diet. Addition of urea decreased body fat less than an equivalent amount of protein.

5. There was no correlation between body fat and body weight in groups fed on diets containing 189 or 216 g protein/kg, but there was a significant correlation between these variables in groups fed on diets containing 292 g protein/kg or either concentration of urea. This phenomenon was much more pronounced in diets not supplemented with methionine.     

Egg production of light and medium hybrids given diets varying in energy level during the chick,rearing and laying stages

A total of 1000 birds, one‐half of which were light and the other half medium hybrids, were given diets containing either high or low levels of metabolisable energy ad libitum during the chick (0.6 weeks), rearing (6–16 weeks), early laying (first 8 months) and late laying (last 4 months) stages.

The medium hybrids ate more and were heavier than the lighter hybrids at all stages. More eggs were laid by the light than by the medium hybrids but the latter laid larger eggs so that the total weight of eggs laid did not differ significantly between the two groups.

Medium hybrids given a low‐energy chick diet laid more eggs subsequently than those given a high‐energy chick diet, while the opposite result was obtained for the light hybrids.

Birds given a low‐energy rearing diet were lighter at 16 weeks and subsequently laid more eggs than birds reared on a high‐energy diet.

During the first part of the laying period consumption of the low‐energy diet was greater than that of the high‐energy diet, but the level and efficiency of egg production were the same for both dietary treatments. Mortality during lay was not significantly affected by dietary treatment or breed.     

Milk production and composition,food consumption,and energy balance of postpartum crossbred Holstein-Gir dairy cows fed two diets of different energy levels

The objective of this study was to evaluate the production, consumption, and energy balance parameters of primiparous 3/4 and 7/8 Holstein × Gir (HG) dairy cows fed two diets of differing energy levels during the postpartum period. At the beginning of the study, 28 days prepartum, the average weight of both genetic groups was 498?±?12 kg and body condition score (BCS) was 3.5?±?0.05. At the end of the study, 61 days postpartum, the 3/4 HG cows had higher weight and body condition scores than the 7/8 HG (456?±?8 and 429?±?8 kg and 3.13?±?0.03 and 2.94?±?0.03 BCS for 3/4 HG and 7/8 HG, respectively). Milk from cows fed the high-energy diet had higher percentages of fat, protein, lactose, and total dry extract than cows fed the low-energy diet. Cows fed the high-energy diet had higher net energy intake (95.3?±?1.9 vs. 88.1?±?2.1 MJ/day) and higher energy balance (3.64?±?2.13 vs ??6.02?±?2.30 MJ/day). The 3/4 HG cows displayed higher energy for maintenance (33.1?±?0.4 MJ/day) than the 7/8 HG (31.5?±?0.5 MJ /day). In conclusion, although the primiparous 3/4 HG were heavier than the 7/8 HG and had a higher body condition score, no differences in milk produced up to 60 days postpartum were observed. The higher energy diet during the postpartum period increased energy balance, resulting in higher production of milk fat, protein, and lactose.

     

Zinc, copper and iron utilisation by chicks fed various concentrations of zinc

1. Zinc, iron and copper interactions were examined in chicks (New Hampshire X Single Comb White Leghorn) grown individually in stainless steel cages and fed on purified diets with three concentrations of zinc: control (37 mg Zn/kg), moderate (100 mg Zn/kg) and excess (2000 mg Zn/kg) in three studies. 2. Chicks given either moderate or excess zinc excreted more than twice as much 65Zn from a test diet as those fed on the control diet. Thus chicks given the moderate amount of zinc had concentrations of zinc in tissues similar to those of controls but chicks given excess zinc accumulated zinc in their tissues. 3. The specific activity of 65Zn in the tissues, especially in the bursa of Fabricius, of chicks given excess zinc was not reduced as much as would be predicted. Thus, ingestion of excess zinc appeared to reduce tissue turnover of 65Zn, especially in the bursa. However, humoral immune responses, as measured by antibody titres to sheep red blood cells, were not affected. 4. Chicks given excess zinc or pair-fed on the control diet retained less 59Fe from a test diet than chicks given control or moderate concentrations of zinc ad libitum. The iron concentrations in tibiae of chicks given excess zinc were depressed.     

A comparison of the energy and nitrogen metabolism of broilers selected for increased growth rate,food consumption and conversion of food to gain

1. Calorimetric measurements were made on 5‐week‐old male chickens sampled from the third generation of three lines selected for either increased live‐weight gain (W), food consumption (F), or food conversion efficiency (E). A control line (C) was also measured.

2. Food intake and food conversion ratio were greater (P<0.05) in the F line than in the E and C lines.

3. Metabolisability of the diet was 0.8% higher in the E line than in the other lines.

4. Metabolisable energy (ME) intake and heat production were greater (P<0.05) in the F line than in the E and C lines, and energy balance was greater (P<0.05) in the F than in the W and E lines.

5. During starvation, excreta energy and heat production were greater (P < 0.05) in the F than the other lines.

6. Availability of ME (net energy) was the same (85%) for all lines but calculated daily maintenance energy requirements (kJ ME/kgW) were W, 860; F, 937; E, 796 and C, 810.

7. By 9 weeks the F line contained more fat and less water than lines E and C.     

Comparison of the retention in chicks and mice of 59Fe given orally as chloride, ethylene diamine tetra-acetate or nitrilotriacetate and in chicks given diets composed of conventional ingredients or semi-purified nutrients

In experiment 1 chicks and growing mice given diets containing 400 mg Fe kg-1, added as chloride, ethylene diamine tetra-acetate or nitrilotriacetate (NTA), were dosed orally with 59Fe in the form present in the diet and the quantities of 59Fe in liver, spleen, blood, remaining carcase and whole blood were calculated as concentration in fresh tissue and as a percentage of the dose. Retention as concentration of 59Fe in fresh tissue was significantly greater in mice than chicks for the spleen and blood (P less than 0.001) and greater in chicks than mice for the liver (P less than 0.05) and carcase (P less than 0.01). Overall differences from the form of iron were significant for the spleen, carcase and whole body (P less than 0.01). These were given primarily by high values for chloride and low for NTA. Percentage retention values gave a similar overall picture to that for concentration. In experiment 2, chicks given diets based on conventional ingredients or semi-purified nutrient with supplements providing 400 mg Fe kg-1 were dosed orally with 59Fe and retention was determined in liver, spleen, blood, remaining carcase and whole body, as in experiment 1. Retention as concentration was significantly greater for the semi-purified nutrient diet than the conventional one in all tissues, at P less than 0.01 in liver and P less than 0.001 in other tissues. There were no significant overall effects from the form of iron added as the supplement.     

Nutrient digestibility and energy utilisation of diets based on wheat, sorghum or maize by the newly hatched broiler chick

1. Two experiments were conducted to measure changes in nutrient utilisation in the newly hatched broiler chicken. The first experiment determined the nitrogen-corrected apparent metabolisable energy (AME(N)) and total tract digestibility of starch and fat in diets based on wheat, sorghum and maize during the first two weeks post-hatch. The second experiment was conducted to confirm the results of Experiment 1 using wheat- and maize-based diets and was of 21 d duration. 2. In both experiments, changes to AME(N) with age were similar irrespective of the diet type, declining from d 3 to d 5-9 and then increasing after d 9. 3. In Experiment 1, the total tract digestibility of starch and fat exhibited a pattern similar to that of AME(N). 4. In Experiment 1, cereal effects were significant, with maize- and sorghum-based diets having higher AME(N) values than the wheat-based diet. Total tract starch digestibility determined for d 5, 7 and 14 showed no cereal differences. Total tract fat digestibility on d 7 was significantly lower for the wheat- and sorghum-based diets than for the maize-based diet, but no cereal differences were observed on d 5 or 14. 5. In Experiment 2, cereal effects were significant, with the maize-based diet having a higher AME(N) than the wheat-based diet. 6. These results showed that nutrient utilisation is compromised during the first week of life of the broiler chick.     

Effect of moderate methionine deficiency on food, protein and energy utilisation in the chick

The effects of feeding a diet marginally deficient in methionine to chicks have been examined. Compared with methionine‐supplemented diets the methionine‐deficient diet caused increased food intake, less efficient food conversion, decreased nitrogen retention and increased uric acid excretion; measurement of plasma uric acid concentration and liver xanthine dehydrogenase activity also indicated a more active state of uric acid synthesis in methionine‐deficient chicks. The increased food intake in response to a moderate deficiency of methionine is commented upon and the implications of the increased synthesis of uric acid in relation to the fate of the increased energy ingested is discussed.     

Effects of mannanoligosaccharide in broiler chicken diets on growth performance, energy utilisation, nutrient digestibility and intestinal microflora

1. A study was undertaken to evaluate the effects of mannanoligosaccharide (MOS, Bio-MOS, Alltech Inc.) on the growth performance, energy utilisation, nutrient digestibility and intestinal microflora of birds given a sorghum-wheat based diet. Two MOS levels (1 and 2 g/kg) were included in the diet. 2. Inclusion of MOS at both levels in the diet improved the apparent metabolisable energy (AME) values of the diet. However, these effects were not as pronounced as those of zinc bacitracin (ZnB) treatment. Dietary ZnB also significantly improved the net energy value of the diet. No significant differences between the different levels of MOS were noticed in the growth performance, AME and net energy values of the diet. Compared to the negative control, inclusion of 2 g/kg MOS tended to improve feed conversion efficiency (FCE) in the starter phase. 3. Dietary MOS did not affect the apparent total tract digestibility of nutrients compared to the negative control. In contrast, ZnB significantly improved the protein digestibility and tended to increase the starch digestibility. The addition of MOS reduced the concentration of arabinose in the soluble non-starch polysaccharides (NSP) fraction in the excreta of birds; whereas, the concentrations of individual sugars in the insoluble NSP and free sugar fractions were increased by ZnB. 4. A decrease in the populations of lactobacilli and coliforms in the ileal and caecal lumen was observed for MOS and ZnB treatments. Correspondingly, pH and microbial fermentation in the gut was altered. The addition of MOS tended to reduce the coliform load at the gut mucosa. 5. Results from the current study suggest that MOS can improve the apparent energy utilisation of the diet and tend to improve FCE of birds in the first three posthatch weeks, which may be partly related to the modulatory effects of MOS on the gut microflora.     

Metabolisable energy,nitrogen absorbability and feeding value of expeller processed mustard cake for chicks

Four experiments were carried out to compare the nutritional indices of expeller processed mustard cake (MS) with that of groundnut cake (GN). The indices measured were metabolisable energy (ME), nitrogen absorbability and growth rate. The average ME values of MS and GN were 2.19 Mcal/kg (9.16 MJ/kg) and 2.75 Mcal/kg (11.51 MJ/kg) respectively. The apparent nitrogen absorbabilities were 63.3% and 67.5% respectively, when the chicks were fed on assorted sources of protein containing either of the cakes but were 70.8% and 73.7% respectively when these cakes served as the sole source of protein in the diet. Although ME content and nitrogen absorption of MS were lower than GN its feeding value was similar when growth and food conversion were compared.     

Effect of diet form and enzyme supplementation on growth, efficiency and energy utilisation of wheat-based diets for broilers

1. The effect of diet form (mash, cold-pelleted, steam-conditioned/pelleted, wet mash, whole wheat with balancer pellet, restricted pellet) and enzyme inclusion (Avizyme 1300, absent, present) was studied in 2 trials using individually caged, male broilers from 14 to 42 d. Bird performance, viscosity of ileal contents and diet metabolisability (AME) were measured. 2. The performance of mash-fed birds was significantly poorer than for the other treatments in relation to dry matter intake, liveweight gain and gain:food. This was not due to reduced diet AME content. 3. There was no significant effect of heat treatment on any of the variables measured, although viscosity of ileal contents was increased by 30% as compared to the cold-pelleted diet. 4. Gain:food was improved with wet-mash feeding in comparison to the dry mash treatment but it was concluded that this was not due to any intrinsic improvement in diet quality, but rather to voluntary food restriction on introduction of the wet food. 5. Whole wheat feeding improved gain:food and diet AME content by 3% as compared to the complete diets and caused approximately a 50% increase in gizzard weight as compared with the pelleted diets. 6. Food enzyme inclusion did not improve performance although a significant improvement in diet AME content was observed with enzyme inclusion in trial 1.     

Rates of muscle protein breakdown in chickens selected for increased growth rate, food consumption or efficiency of food utilisation as assessed by N tau-methylhistidine excretion

1. N tau-methylhistidine excretion, growth rate, food consumption and body composition was determined in 12 4 to 5 week old chickens sampled from each of 4 lines selected for increased body-weight gain (line W), for increased food consumption (line F), for improved efficiency of food utilisation (line E) or at random (line C), after 12 generations of selection. 2. The use of N tau-methylhistidine as an index of myofibrillar protein breakdown was validated in male and female chickens of lines E and F by following the fate of injected N tau-(14CH3)methylhistidine. Most of the radioactivity (79.3 +/- 1.1%) was excreted in 4 d, with the remainder retained in the carcase. In excreta, 94 +/- 2% of the radioactivity was associated with free N tau-methylhistidine and for the carcase, this value was 88 +/- 3%. 3. In the main experiment, final body weights averaged 497, 651, 588 and 537 g and food: gain ratio averaged 2.47, 2.21, 3.14 and 2.06 for lines C, W, F and E respectively, Carcase protein content (g/100 g body weight) was not different between the lines. 4. N tau-methylhistidine excretion was 5.86, 5.48, 6.43 and 4.99 mumoles/mole carcase-N/d for lines C, W, F and E, respectively. The rate for line F was significantly higher than for lines W and C and that for line E was significantly less than for the control line. 5. The N tau-methylhistidine excretion rate was positively correlated with food: gain ratio. 6. Selection for rapid growth, high food consumption or improved food utilisation results in changes in N tau-methylhistidine excretion which suggest proportionate changes in muscle protein turnover.     

Effect of feeding diets based on coarse maize on productive performance,gizzard development and energy utilisation of laying hens

1. A total of 2200 White Leghorn layers were used to study the effect of feeding coarse maize on productive performance, gizzard weight, apparent metabolisable energy (AME) and egg quality parameters.

2. The experiment was a completely randomised design with 5 treatments, each being replicated 5 times (88 birds per replicate). Dietary treatments included a control diet with 600 g/kg of ground maize (6 mm sieve) and experimental diets with 150, 300, 450 or 600 g/kg coarse maize (10 mm sieve) replacing (w/w) ground maize. Diets, in mash form, were offered from 39 to 62 weeks of age.

3. Over the entire experimental period, dietary treatments had no effect on any of the production parameters, except on feed intake. A quadratic effect was observed for feed intake, where intake increased at 150 g/kg coarse maize inclusion. At higher inclusion levels of coarse maize, feed intake was similar to that of the control diet. But the differences in daily intake between dietary treatments were only around 1 g/bird and may not be of any biological significance.

4. Dietary treatments had no effect on gizzard weight, AME or egg quality.

5. The results indicate that coarse maize could completely replace maize in layer diets with no adverse effect on egg production, egg quality and dietary energy utilisation. In the current work, although the proportion of coarse particles (over 1 mm) increased with increasing inclusion levels of coarse maize, the differences in the percentage of particles above 1 mm between the fine control (0 g/kg coarse maize) and coarsest (600 g/kg coarse maize) diets was only 15%, and this may limit the value of the findings reported herein.     

A comparative study of energy utilisation by the laying hen from diets containing a high proportion of fat and diets made up mainly from carbohydrate sources

An experiment was carried out in which laying hens were fed diets containing about 30 per cent tallow at three protein levels. Productive performances of hens on these diets were compared with those of hens fed either a diet containing 15 per cent arachis oil, a diet containing 15 per cent sucrose or a conventional cereal‐based control diet.

It was found that the best performance was obtained with hens fed the control diet. Gross energetic efficiency of egg production was slightly lower in the birds fed the diets containing arachis or sucrose. It appeared that the lower efficiency was due to the higher mean body weight of birds on these diets. Egg size was significantly increased by feeding the diet containing arachis oil.

Performance of hens given diets containing tallow was unsatisfactory. For each diet egg production was significantly less than that recorded for the control diet and the gross energetic efficiency of egg production was much lower than that on the control diet.     

Comparison of the utilisation of palm kernel meal,brewers' dried grains and maize offal by broiler chicks

1. Palm kernel meal (PKM), brewers dried grains (BDG) and maize offal (MO) were included in broiler diets, each at 100, 150 or 200 g/kg; the diets were fed up to 35 d of age. 2. Overall food intake and weight gain decreased in the order BDG, PKM and MO. There were, however, significant interactions between the test ingredients and dietary concentrations in all the growth responses. Food intakes increased with the dietary concentrations of each test ingredient, but the increase was greater for BDG than PKM or MO. For weight gain, at 100 g/kg, the final body weights of the chicks fed on the diets with BDG and MO were similar, and those of chicks fed on the diet with PKM slightly lower. However, at 200 g/kg, growth rate of chicks fed on the BDG and PKM diets were similar while those of chicks fed on the MO diet was 7% lower. Efficiency of food utilisation was similar at 100 g/kg for all the ingredients and decreased as their concentrations increased; however, the decrease was considerably less for the PKM than for the MO and BDG diets. 3. Broilers fed on the BDG-based diets voided most excreta followed by those fed on the PKM and MO diets; excreta water content was highest from birds fed on the MO diets followed by the PKM and BDG diets. Apparent retention of dry matter was similar with all the test ingredients, but it decreased only significantly at 200 g/kg dietary concentration. The rate of passage was faster with the PKM diets followed by the MO and BDG diets; it was increased at 200 g/kg dietary concentration of the test ingredients.     

Relationship of energy, phosphorus and breed of chicken to growth and food efficiency

Three trials were conducted to determine the response of two breeds of chickens (Single Comb White Leghorn—SCWL—and commercial crossbred broiler type) to various dietary energy and phosphorus levels. Dietary energy level, dietary phosphorus level and breed each had a consistent highly significant (P<0.01) effect on body weight at 4 weeks of age. A number of significant (P<0.05) and highly significant (P < 0.01) interactions occurred with body weight. The energy x phosphorus interaction was significant (P < 0.05) in the three trials which supports the conclusion that, in the heavy breed at least, the higher the energy level of the diet, the greater the phosphorus requirements. The phosphorus x breed interaction was highly significant (P <0.01) in the three trials supporting the conclusion that the two breeds differed in their phosphorus requirements. The energy x breed interaction was less consistent being highly significant (P < 0.01) in one trial, significant (P < 0.05) in another, and non‐significant in a third. Dietary energy level, breed, and energy x breed were the most consistent factors influencing food‐to‐gain ratio. Mortality was high (20 per cent) when the broiler‐type chickens were fed low phosphorus, low energy diets and increased with each increase in the energy level of the diet. No treatment effect on mortality was observed with the SGWL.     

The effect of dietary energy:protein ratio,protein quality and food allocation on the efficiency of utilisation of protein by broiler chickens

1. Various theories have been proposed to explain the reduced performance of broilers when given feeds excessively high in protein, but a satisfactory solution to this problem had, up to now, not been found. Three experiments were conducted to test the hypothesis that the efficiency of utilisation of protein (e_p) is a linear–plateau function of the ratio between the feed apparent metabolisable energy and digestible crude protein contents (AMEn:DCP) and that dietary protein quality, feed allocation and sex do not influence this relationship.

2. A ‘linear–plateau’ model successfully described the efficiency of protein utilisation (e_p) as a function of AMEn:DCP in all three experiments. In Experiment 1, with both sexes being both ad libitum and control fed, the breakpoint was at 58.6 MJ AMEn/kg DCP. In Experiment 2, both sexes were fed balanced and unbalanced protein series, and at different rates, the slopes of the ascending part of the linear–plateau relationships for the different treatments were the same for all treatments (0.0204), and the inflection point was at 71 MJ AMEn/kg DCP. Using similar treatments in Experiment 3, the breakpoint for the balanced protein was 72 MJ/kg and for the unbalanced, 64, with a combined slope of 68 MJ AMEn/kg DCP.

3. The three experiments provide adequate evidence that e_p is a linear–plateau function of the dietary AMEn:DCP ratio with a breakpoint of around 66.2 ± 1.98 MJ AMEn/kg DCP. Below this critical ratio, food intake declines as does protein and lipid retention.

4. That broilers, like pigs, exhibit an energy-dependent phase when high-protein feeds are offered is of practical importance when formulating pre-starter feeds for broilers and starter feeds for turkey poults as the e_p of such feeds may well fall below the maximum due to the lack of dietary energy required to process the high dietary protein contained in such feeds resulting in poorer performance than expected.