共查询到20条相似文献,搜索用时 640 毫秒
1.
实验用光学显微镜和透射电镜观察了海狸鼠卵巢卵母细胞在体外成熟培养前后的形态及超微结构。结果表明:在成熟培养前,海狸鼠卵细胞微绒毛多插入透明带,有的的退化;皮层颗粒多数散在分布皮质区;线粒体多数成行排列分布于皮质区或细胞核周围,线粒体多为椭圆形、多嵴;细胞核位于皮质区,核膜有时打褶。成熟培养后,微绒毛从透明带中撤出,倒伏在质期上;皮层颗粒多成行排列于质膜下,线粒体开始散在分布于胞质中;高尔基体和粗面 相似文献
2.
为比较猪卵母细胞在GV期与MⅡ期的冷冻保存效果,试验在这两个成熟阶段对其进行玻璃化冷冻,GV期卵母细胞解冻后培养至成熟,MⅡ期卵母细胞解冻后恢复2 h,然后采用免疫荧光标记、Western blotting和链霉蛋白酶溶解方法分别检测它们的皮质颗粒分布、CD9蛋白表达水平和透明带消化时间上的差异。结果表明,GV期卵母细胞在解冻后2 h的存活率显著低于MⅡ期卵母细胞(P<0.05),但极体排出率与对照卵母细胞无明显差异(P>0.05);在冷冻MⅡ期卵母细胞中,皮质颗粒的皮质区分布比例和CD9的蛋白表达水平显著下降(P<0.05),但冷冻GV期卵母细胞经体外成熟后则无明显变化(P>0.05);冷冻GV期与MⅡ期卵母细胞均不会影响透明带的消化时间(P>0.05)。由此可见,猪卵母细胞在GV期的冷冻存活率虽然较MⅡ期低,但其体外成熟后极体排出率、皮质颗粒分布和CD9蛋白表达水平均未受到冷冻的影响。 相似文献
3.
试验旨在探究玻璃化冷冻及培养过程中添加甘氨酸(glycine,Gly)对水貂GV期卵母细胞冷冻解冻后存活率、核发育、线粒体和皮质颗粒分布的影响。试验分为3组:对照组(没有进行冷冻处理)、冷冻组和Gly添加处理组(1 mmol/L Gly)。对玻璃化冷冻解冻后的水貂GV期卵母细胞分别进行平衡恢复3 h和体外成熟培养,采用免疫荧光标记法检测各组GV期卵母细胞线粒体分布的差异及MⅡ期皮质颗粒分布的变化。结果显示,Gly添加处理组卵母细胞在解冻后3 h的存活率与冷冻组相比差异不显著(P0.05),但显著低于对照组(P0.05);Gly添加处理组卵母细胞的减数分裂恢复率显著高于冷冻组(P0.05),但与对照组相比差异不显著(P0.05)。免疫荧光结果显示,Gly添加处理组的GV期卵母细胞线粒体正常分布率显著高于冷冻组(P0.05),但Gly添加处理组和冷冻组的GV期卵母细胞线粒体正常分布率均显著低于对照组(P0.05)。皮质颗粒分布结果显示,水貂GV期卵母细胞在冷冻后体外成熟培养至MⅡ期时,Gly添加处理组皮质颗粒的正常皮质区分布比例显著高于冷冻组(P0.05),但Gly添加处理组与冷冻组的正常皮质区分布比例均显著低于对照组(P0.05)。结果表明,添加Gly可以提高冻融后水貂卵母细胞的减数分裂恢复率,降低冷冻对其线粒体及皮质颗粒的损失。 相似文献
4.
体外培养牛腔前卵泡卵母细胞的超微结构 总被引:1,自引:1,他引:0
直径约100μm牛腔前卵泡在无血清下体外培养15d。超微结构研究显示,培养前腔前卵泡卵母细胞微绒毛短小,分布均匀。胞质内细胞器致密而均匀,线粒体多为长或圆形,嵴较少,高尔基体、脂滴稀少,未见有皮质颗粒出现。培养6d时,微绒毛略变粗长,但数量减少。胞质内细胞器,由近核分布向胞质中央区转移,线粒体形态丰富,基质电子密度极高。培养15d腔前卵泡卵母细胞微绒毛增多,变长,斜向或垂直插入已经形成的透明带中。线粒体增多,大小不等,形态各异,胞质中区有溶酶体样物质及零星的皮质颗粒出现。电镜研究表明,体外培养与体内正常发育腔前卵泡卵母细胞超微结构变化规律基本相似。 相似文献
5.
试验旨在探究玻璃化冷冻及培养过程中添加甘氨酸(glycine,Gly)对水貂GV期卵母细胞冷冻解冻后存活率、核发育、线粒体和皮质颗粒分布的影响。试验分为3组:对照组(没有进行冷冻处理)、冷冻组和Gly添加处理组(1 mmol/L Gly)。对玻璃化冷冻解冻后的水貂GV期卵母细胞分别进行平衡恢复3 h和体外成熟培养,采用免疫荧光标记法检测各组GV期卵母细胞线粒体分布的差异及MⅡ期皮质颗粒分布的变化。结果显示,Gly添加处理组卵母细胞在解冻后3 h的存活率与冷冻组相比差异不显著(P>0.05),但显著低于对照组(P<0.05);Gly添加处理组卵母细胞的减数分裂恢复率显著高于冷冻组(P<0.05),但与对照组相比差异不显著(P>0.05)。免疫荧光结果显示,Gly添加处理组的GV期卵母细胞线粒体正常分布率显著高于冷冻组(P<0.05),但Gly添加处理组和冷冻组的GV期卵母细胞线粒体正常分布率均显著低于对照组(P<0.05)。皮质颗粒分布结果显示,水貂GV期卵母细胞在冷冻后体外成熟培养至MⅡ期时,Gly添加处理组皮质颗粒的正常皮质区分布比例显著高于冷冻组(P<0.05),但Gly添加处理组与冷冻组的正常皮质区分布比例均显著低于对照组(P<0.05)。结果表明,添加Gly可以提高冻融后水貂卵母细胞的减数分裂恢复率,降低冷冻对其线粒体及皮质颗粒的损失。 相似文献
6.
【目的】探究貉卵母细胞成熟发育特征,为貉卵母细胞的体外成熟培养及优质成熟卵母细胞的挑选提供依据,以提高貉的体外受精效率。【方法】采集1~3岁(性成熟)繁殖期母貉的新鲜卵巢,制备光、电镜样品,观察貉卵母细胞成熟发育过程中细胞器变化;利用X射线微分析技术探索卵母细胞质膜化学元素变化与成熟发育的关系。【结果】根据颗粒细胞层数、卵泡直径、卵母细胞大小以及透明带的变化,将貉卵母细胞成熟发育划分为8个时期。貉卵母细胞在早期发育阶段(第Ⅰ~Ⅳ期)由颗粒细胞包围,逐渐出现透明带、卵泡腔,生发泡内的染色质越来越致密;随着卵母细胞的成熟发育(第Ⅴ~Ⅶ期),微绒毛、皮质颗粒出现;高尔基复合体、线粒体等细胞器数量明显增加,且逐渐迁移至皮质区,同时线粒体、脂滴形态发生变化。卵母细胞成熟时(第Ⅷ期),高尔基复合体及粗面内质网消失,皮质颗粒在质膜下排列;排卵后,核仁发生致密化。随着卵泡腔的增大,卵母细胞质膜表面的钠、氯和硫含量呈下降趋势,第Ⅴ期卵母细胞质膜中钠、氯和硫含量显著高于第Ⅶ期(P<0.05),钙含量显著低于第Ⅵ期和第Ⅶ期卵母细胞(P<0.05);钾含量呈现低-高-低的变化规律,第Ⅵ期卵母细胞质... 相似文献
7.
8.
为研究血管内皮生长因子(vascular endothelial growth factor,VEGF)对绵羊卵母细胞体外成熟过程中细胞超微结构变化的影响,根据前期研究结果,在绵羊卵母细胞体外成熟培养液(TCM199+BSA)中添加不同剂量的VEGF(对照组0 ng.mL-1和试验组5 ng.mL-1)进行成熟培养。利用透射电镜技术进行分析,研究结果显示:①VEGF加速了微绒毛的发育和变化,促进了微绒毛游离端斜行插入透明带,增加了细胞内外物质交流和养分的吸收;②VEGF对卵母细胞成熟过程中高尔基体的影响不显著;③发现特征性绵羊卵母细胞线粒体———带帽状线粒体,并且VEGF对线粒体的迁移有一定的促进作用;④VEGF的添加对绵羊卵母细胞体外成熟过程中脂滴数量的增加没有显著的作用,但VEGF减少了小脂滴聚集为大脂滴的数量。 相似文献
9.
10.
11.
12.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
13.
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
16.
17.
18.
19.
以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
20.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献