植物液泡中硝酸盐行为的研究概况

化学氮肥利用率低和损失严重而造成的环境污染问题是农业生态系统中氮素管理首当其冲要研究和解决的问题 ,这方面的研究工作在国内外报道浩如烟海 ,但突破性进展不多。另一方面 ,植物体内的硝酸盐含量高又严重影响农产品有关的品质性状 ,特别是我国加入WTO后蔬菜和果实中高含量的硝酸盐是影响这些产品出口的主要限制因子之一。因此 ,从植物体本身着手研究植物氮素高效利用的机理与途径 ,这是近几年来植物氮素营养研究的热点之一。植物液泡占据了成熟植物细胞体积的 90 %左右 ,而液泡和细胞质中硝酸盐的浓度通常分别在 30～ 5 0molm- 3和 3～ 5molm- 3,因此 ,如何调动植物液泡中的硝酸盐使之得到更高程度的再利用 ,这是提高植物氮素利用效率和降低植物体内硝酸盐含量的途径之一。本文综述了国内外有关液泡中硝酸盐行为的研究状况 ,在此基础上作者首次提出植物液泡中硝酸盐的内外流与植物氮素高效利用之间可能存在着密切的关系 ,旨在拓始这方面的工作能尽快开展 ,为植物氮素高效利用的分子生物学研究开辟新的研究领域。     

设施菜田不同施氮处理对硝酸盐迁移和积累的影响

在设施菜地条件下,研究了氮肥减施及配施抑制剂处理在黄瓜生长期对土壤NO3--N迁移累积的影响。结果表明,氮肥减施处理可显著降低土壤表层和整个土体的NO3--N含量。常规施氮量时0~40 cm土层的NO3--N含量均高于其它处理,减氮30%后0~40 cm土层未出现NO3--N显著积累现象;氮肥配施抑制剂处理不同程度降低了土壤NO3--N含量,且抑制硝态氮向下层土壤淋失,其中抑制剂组合的效果最好。氮肥配施抑制剂,可以有效控制NO3--N在土壤和植物体内的过量累积,减少硝态氮淋溶损失。     

黄土高原丘陵沟壑区植被恢复过程对土壤剖面矿质氮累积影响--以纸坊沟流域试验区为例

根据不同植被类型和不同植被恢复年限,在位于半干旱黄土高原丘陵沟壑区延安安塞纸坊沟流域采集68个剖面样品,探讨植被恢复过程土壤剖面中残留矿质态氮的变化;同时采取该流域连续14年施用不同肥料处理的坡地长期定位试验剖面土样,研究连续施肥对农田土壤剖面残留NO3--N累积的影响。结果表明,NH4 -N在土壤剖面中的分布和累积基本不受植被恢复及植类型的影响,但NO3--N在土壤剖面中的累积量随植被恢复而下降。林地、草地和农田0～50cm土层平均累积的NO3--N分别为17 4kg/hm2,14 9kg/hm2和39 9kg/hm2;林地和草地剖面中NO3--N累积量所占矿质氮总累积量比例远小于NH4 -N,而对农田土壤,剖面中NO3--N累积量所占比例与NH4 -N所占比例基本相当;农田土壤剖面中NO3--N累积量所占比例显著大于林地和草地。长期定位试验结果进一步证明了在农田连续施用氮肥会显著增加土壤剖面中残留NO3--N累积,当农田退耕还林还草后,累积的这一部分NO3--N因植物吸收利用、土壤生物固定和损失等途径而下降,最终达到低而稳定的水平。     

氮肥投入水平对蔬菜地硝态氮淋洗特征的影响

通过3年的田间定位试验研究了不同施N条件下蔬菜地NO3--N淋洗浓度及淋洗量的变化。结果表明:在农民习惯的传统施N处理(花椰菜为450kg/hm2,苋菜为100kg/hm2,菠菜为309kg/hm2)下蔬菜地NO3--N的淋洗浓度明显高于2个优化施N处理下蔬菜地的NO3--N淋洗浓度。在花椰菜、苋菜和菠菜生长期内,2个优化施N处理下蔬菜地NO3--N平均淋洗量分别是传统施N处理下蔬菜地NO3--N平均淋洗量的19%、18%、9%和13%、34%、21%。试验期间传统施N处理下蔬菜地NO3--N年季平均累积淋洗量约占年季平均施N量的一半,其中休闲期NO3--N平均累积淋洗量占年季平均累积施N量的20%;而其他2个优化施N处理NO3--N年季平均累积淋洗量是年季平均累积施N量的27%,休闲期NO3--N平均淋洗量占年季平均累积施N量的6%,而处理间蔬菜产量并未受到明显影响。     

液泡膜苹果酸转运蛋白研究进展

液泡是植物细胞贮藏苹果酸重要的细胞器。苹果酸是三羧酸酸循环和乙醛酸循环的中介,是维持细胞渗透压与电荷平衡的关键代谢物,还参与调节植物气孔大小,故苹果酸在植物的生命活动中起着重要作用。液泡膜苹果酸转运蛋白直接或间接控制苹果酸进出液泡,介导液泡与细胞质间苹果酸的运输。液泡膜苹果酸转运蛋白属于钠连接的羧酸盐载体家族,本文重点介绍植物液泡膜苹果酸转运蛋白的性质和功能及其与植物细胞pH值动态平衡之间关系的研究进展。     

施用磷肥对土壤NO3——N累积的影响

在黄土高原南部的国家黄土肥力和肥料效益监测基地进行的长期定位试验结果表明 ,在小麦 玉米轮作中 ,当年施氮量为N 352kg/hm2 时 ,单施氮肥或氮钾配合的 0～4m土壤剖面的NO3--N累积量达 1000kg/hm2 以上 ,其中约 50%～60%的NO3--N分布在 2～ 4m以下的土层中 ,而氮磷配合的 0～ 4m土壤剖面的NO3--N累积量仅为 220kg/hm2,且 80 %的NO3--N分布在 0～2m的土层中 ,增施磷肥由于增加了氮的吸收和对水分的利用而有效地降低了土壤中NO3--N的累积。     

施肥对日光温室黄瓜和土壤硝酸盐含量的影响

通过田间试验研究了不同施肥对日光温室黄瓜NO2--N和NO3--N含量和土壤NO3--N以及黄瓜产量的影响。结果表明,在黄土高原黄绵土上,施N400kg.hm2和P2O5250kg.hm2,黄瓜生长期间,NO3--N含量变化与黄瓜的生长发育阶段关系密切,黄瓜结瓜前020和2040cm土层NO3--N含量较高,随黄瓜生长速度加快和结瓜盛期的到来,土壤NO3--N含量降低;黄瓜收获后,NO3--N含量又有增加。不同施肥种类比较,施用化肥40160cm土层NO3--N的累积和淋洗量最大,施用沼肥其累积和淋洗量小于施用化肥,而施用有机肥(牛粪)NO3--N的累积和淋洗量小于施用沼肥。采用叶面喷施尿素和有机钾肥,可以减少化肥和有机肥用量,从而降低土壤剖面0200cmNO3--N的累积。使用沼肥、叶面肥的黄瓜产量都明显高于不施肥和NP化肥处理。     

降水条件下黄土坡地氮素淋溶特征的研究

坡地氮素淋溶是导致坡地土壤质量退化和农业非点源污染的重要原因之一。为了深入理解坡地氮素淋溶特征,采用了人工模拟降雨和天然降雨观测方法,对黄土坡地氮素淋溶特征进行了试验研究。结果表明:从坡底到坡顶土壤NO3--N含量呈不断衰减的波浪形状变化,坡底有明显NO3--N累积,其累积含量占全坡面37%~52%;坡地土壤NO3--N淋溶是二维迁移,即随着入渗水既向土体深层迁移,又向坡底迁移;降雨量分别与NO3--N淋溶深度和淋失量均呈正相关,大约每4 mm降雨量可使NO3--N下渗1 cm。本研究为建立坡地养分运移模拟模型提供了初步设想。     

旱地土壤硝态氮残留淋溶及影响因素研究

在我国北方旱地,施入土壤而未被作物吸收利用的肥料N,主要以NO3--N的形式残留于土壤中。残留的NO3--N如不及时被作物吸收利用,在降水或灌水的作用下,会淋入土壤深层,或随径流进入地表水体,或经反硝化形成N2O进入大气,对土壤、水体和大气环境构成严重威胁。本文分析了旱地农田生态系统中,NO3--N在土壤剖面的残留淋溶与施肥、灌溉/降水、耕作、土壤、植物等因素的关系。提出在今后的研究工作中应特别注意的问题:①建立长期定位试验,确定NO3--N淋溶阈值,评价和预测NO3--N残留和淋失的趋势;②优化作物栽培和养分资源管理措施,提高作物利用土壤NO3--N的能力;③改进N肥施用技术,加强N素管理,防止NO3--N在土壤中大量累积。     

不同施肥及杂草处理下半湿润冬小麦田NO3——N的动态变化

以中等肥力土垫旱耕人为土为供试土壤,在冬小麦（Triticum aestivum）不同生育期采集0-100 cm土层土壤样品,研究不同施肥及杂草处理对半湿润农田生态系统土壤NO3--N动态变化的影响。结果表明,1）土壤剖面NO3--N含量随施氮量增加而显著增加,0-100 cm土层NO3--N累积量与施氮量线性相关;在越冬期、返青期和拔节期,相关系数r分别为0.995、0.971和0.949。2）冬小麦生长过程中,土体NO3--N含量先降低后回升,在拔节期最低;成熟期土壤有机氮矿化产生的NO3--N是收获后土壤剖面残留NO3--N的主要贡献者。3）在越冬期、返青期、拔节期和成熟期,施磷（PN135）与不施磷（P0N135）处理相比,施磷可显著减少土体NO3--N累积量,减少量分别为N 61.4、26.9、36.6和5.5 kg/hm2;磷肥对减少土壤NO3--N残留累积量的影响以越冬期表现最为显著,成熟期表现不显著。4）在施磷的基础上,不同杂草处理土壤剖面NO3--N累积量在每公顷施氮45 kg（PN45）及施氮90 kg (PN90)时存在一定差异,但不显著;而在每公顷施氮180 kg（即PN180）的高氮处理下,差异显著。每公顷施氮135 kg（PN135）,的中氮处理,在越冬期清除杂草后土壤剖面中NO3--N累积量在拔节期显著高于其它杂草处理。     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.     

Properties of anaerobically digested and composted municipal solid waste assessed by linking soil mesofauna dynamics and nitrogen modelling

We have studied the effect of anaerobically digested (ADMSW) and composted municipal solid waste (CMSW) on mineralization and foodweb dynamics to verify the hypothesis that ADMSW would immobilize N right after addition to soil in contrast to addition with CMSW. Another hypothesis was that the mesofauna (enchytraeids and microarthropods) would stimulate N release from the decomposer community. We measured excretion of -N and urea-N from the mesofauna and hypothesized that enchytraeids would release urea. ADMSW and CMSW were amended to pots with sandy loam and barley. The pots were divided into treatments with or without mesofauna. Mesofauna, plant N and biomass, soil N and ergosterol (fungal biomass) were measured over a 113-day period of four equidistant samplings. Soil respiration, N mineralization and N release by the mesofauna were modelled from concurrent studies. ADMSW- and CMSW-treated soils initially (<20 days) immobilized N. The amendments did not increase plant growth substantially, and this was probably due to N-limitation in the early stages of plant growth. Enchytraeid abundance was about three times higher in ADMSW- than CMSW-treated soils, indicating that ADMSW contained more labile compounds, bacteria, and microfauna. The mesofauna did not affect N-content, but the cumulated -N excreted by the mesofauna was estimated to be substantial and about one-fifth of total plant N in ADMSW. An explanation for this discrepancy might be that in the absence of the mesofauna, other members of the detrivore and microbivore community performed the mesofauna’s function. Neither enchytraeids nor microarthropods excreted urea.     

Faculty positions: Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Faculty positions: Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Variation in quality parameters between and within 14 Nordic tree fruit and berry species

Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.     

Potassium Fixation and Release Characteristics in Rhizosphere and Nonrhizosphere Soils for a Rapeseed–Rice Cropping Sequence

Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L^?1 nitric acid (HNO₃) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L^?1 ammonium acetate (NH₄OAc) and 1.0 mol L^?1 HNO₃ extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.