Xylanase supplementation improves energy digestibility of wheat by-products in grower pigs

Value-added processing of cereals produces high-value fractions for food and bio-processing application and by-products that are used in animal nutrition to reduce feed costs. Wheat by-products contain arabinoxylans that might limit nutrient digestibility. Effects of xylanase supplementation (0 or 4375 U/kg feed) on energy digestibility were studied in a wheat control and by-product diets (30% millrun, middlings, shorts, screenings, and bran) in a 2 × 6 factorial arrangement. The wheat control diet was formulated to contain 3.34 Mcal digestible energy (DE)/kg and 2.8 g apparent digestible lysine/Mcal DE, and included 0.4% chromic oxide as a marker for digestibility. Twelve ileal-cannulated pigs (32.5 ± 2.5 kg) were each fed seven of 12 diets. Faeces and then digesta were each collected for 2 d, and diet digestibility values are reported. Wheat had higher ileal and total-tract energy digestibility than by-products (P < 0.01). Xylanase improved energy digestibilities for by-products (P < 0.001) but not for wheat. Among by-products, ileal energy digestibility was lowest for middlings (62%), then bran < screenings < millrun, and highest for shorts (66%). Xylanase improved (P < 0.05) ileal energy digestibility of millrun by 19% to 76%. Total-tract energy digestibility of millrun improved from 72 to 79% (similar to wheat) with xylanase (P < 0.05). In summary, xylanase improved energy digestibility in the selected wheat by-product diets, indicating that arabinoxylans in wheat by-products limit nutrient digestibility.     

Nutrient digestibility of extruded canola meal in ileal-cannulated growing pigs and effects of its feeding on diet nutrient digestibility and growth performance in weaned pigs

Canola meal (CM) contains less crude protein (CP) and more fiber and anti-nutritional factors such as glucosinolates than soybean meal (SBM) and consequently has a lower nutrient digestibility. Therefore, processing strategies that may increase the feeding value of CM warrant study. In two experiments, the effects of extrusion of Brassica napus CM on apparent (AID) and standardized ileal digestibility (SID) of amino acids (AA), apparent total tract digestibility (ATTD) of gross energy (GE) in growing pigs, and growth performance and diet digestibility in weaned pigs were assessed. Solvent-extracted CM was extruded using a single-screw extruder at three screw speeds: 250 (CM-250), 350 (CM-350), or 450 (CM-450) rpm. In exp. 1, in a double 4 × 4 Latin square, eight ileal-cannulated barrows (initial body weight [BW], 68.1 kg) were fed corn starch-based diets containing 50% CM or extruded CM. The CM sample contained 43.2% CP, 33.2% total dietary fiber (TDF), and 8.9 µmol of total glucosinolates/g on a dry matter (DM) basis. Extrusion increased (P < 0.05) the AID of CP, reduced (P < 0.05) apparent hindgut fermentation of CP, and decreased (P < 0.05) predicted net energy (NE) value of diets. Extrusion increased diet AID and CM SID of most indispensable AA by 3.1 to 5.3%-units. In exp. 2, 200 weaned pigs (initial BW, 8.3 kg) were fed diets containing 20% SBM, CM, or extruded CM starting 2 wk postweaning for 3 wk. The CM sample contained 42.7% CP, 28.3% TDF, and 5.3 µmol total glucosinolates/g DM. Wheat-based diets provided 2.3 Mcal NE/kg and 5.1 g SID Lys/Mcal NE. Dietary inclusion of extruded CM replacing SBM decreased (P < 0.05) diet ATTD of DM, GE and CP, and DE value. Average daily feed intake, average daily gain (ADG), and gain:feed (G:F) of pigs did not differ between extruded CM and SBM diets and were not affected by extrusion, but increasing extruder screw speed linearly increased (P < 0.05) ADG for day 1 to 7 and G:F for the entire trial. In conclusion, extrusion increased diet AID and CM SID of AA but not DE and predicted NE values of CM. However, increasing extruder speed did not further increase the SID of most of the AA of CM in growing pigs. Dietary inclusion of 20% CM or extruded CM did not affect the growth performance in weaned pigs.     

Prediction of in vivo apparent total tract energy digestibility of barley in grower pigs using an in vitro digestibility technique

The DE content within cereal grains can vary 25% mainly due to changes in apparent total tract digestibility (ATTD) of energy. In vitro digestibility techniques have been developed to predict the DE value among feedstuffs. However, these techniques have not been tested properly for their suitability to predict the variation in energy digestibility and DE content within a cereal grain. The objective of the present study was to establish and evaluate an in vitro digestibility technique to predict in vivo ATTD of energy of barley in grower pigs. Barley grain samples (hulled, n = 21) with a large range in quality were collected; the ADF and CP content ranged from 4.5 to 11.4% and 10.0 to 16.4% (DM basis), respectively. The ATTD of energy was determined using barrows (n = 63, 33 +/- 2.1 kg of initial BW) in 2 periods with 6 observations per sample and ranged from 51.9 to 78.5%, with relative errors between 0.4 and 5.0%. A preliminary study, comparing a 2- and a 3-step in vitro digestibility technique using 3 barley samples, indicated that R(2) between in vivo and in vitro energy digestibility was greater using the 3- than the 2-step technique (0.92 vs. 0.76). Therefore, the 3-step in vitro digestibility technique was used solely in subsequent analyses. Briefly, ground barley was subsequently incubated with pepsin for 6 h, pancreatin for 18 h, and cellulase for 24 h. The DM and GE content of samples and residues were measured to calculate digestibility. The in vitro energy digestibility of the 21 barley samples with duplicate measurements ranged from 63.7 to 82.2%, with relative errors between 0.1 and 2.6%. In vitro energy digestibility was strongly related (y = 1.25 x - 25.22; R(2) = 0.81) to in vivo energy digestibility. Finally, a subset of 7 barley samples was analyzed in quadruplicate using the 3-step in vitro technique. The relationship between in vitro and in vivo energy digestibility was very strong (y = 1.23 x - 25.33; R(2) = 0.97) with relative errors between 0.5 and 2.7%. In vitro DE and energy digestibility were perfectly related (R(2) = 1.00). In summary, the 3-step in vitro energy digestibility technique can accurately predict the ATTD of energy in barley in grower pigs. The 3-step in vitro digestibility technique, thus, might be useful as the reference laboratory procedure to calibrate analytical equipment to rapidly predict the ATTD of energy in barley.     

Cereal type and heat processing of the cereal affect nutrient digestibility and dynamics of serum insulin and ghrelin in weanling pigs

The effects of feeding corn or rice, either raw or heat processed (HP), on apparent total tract digestibility (ATTD) and apparent ileal digestibility (AID) of nutrients and on insulin and ghrelin concentrations in the serum were studied in young pigs. Pigs were weaned at approximately 23 ± 3 d of age and weighed 7.4 ± 1.2 kg. Each of the 4 treatments was replicated 9 times, and the experimental unit was a pig individually housed. Pigs (5 males and 4 females/treatment) were fed their respective diets ad libitum from 23 to 47 d of age. At 37 d of age, the effects of dietary treatments on the fasting and postprandial concentrations of insulin and total and acylated ghrelin were studied. The ATTD of OM, GE, and ether extract were, respectively, 4.3, 5.4, and 3.6% greater (P < 0.05) for the rice than for the corn diets, but CP digestibility was not affected. Similar results were observed for AID. Heat processing of the cereal increased (P < 0.05) the ATTD by 2.1% for OM, 3.2% for GE, 7.1% for ether extract, and 2.2% for CP and tended to increase the AID of CP (P = 0.06) and starch (P = 0.09). The postprandial serum insulin response was greater and was more prolonged in pigs fed raw rice than in pigs fed raw corn (P < 0.05). In addition, the effects of HP on serum insulin response were more pronounced with corn than with rice (cereal × HP, P < 0.05). Total ghrelin concentration was not affected by treatment, but acylated ghrelin was greater (P < 0.05) at 6 h postprandially in pigs fed rice than in pigs fed raw corn. Feeding rice and HP corn increased nutrient digestibility and insulin response in the early postprandial period and increased the acylated ghrelin response in the late postprandial period compared with feeding raw corn.     

The effects of dietary fiber level on nutrient digestibility in growing pigs

The objective of this study was to investigate the effects of total dietary fiber level on nutrient digestibility and the relationship between apparent total tract digestibility of total dietary fiber, and soluble dietary fiber, insoluble dietary fiber and available energy. Sugar beet pulp was as the only fiber source. The experiment was designed as a 6 × 6 Latin square with an adaptation period of 7 d followed by a 5-d total collection of feces and urine. Feed intake tended to decrease (P =0.10) as total dietary fiber level increased. The apparent total tract digestibility of dry matter, crude protein and gross energy decreased (P <0.01) when total dietary fiber increased but the digestibility of soluble dietary fiber and insoluble dietary fiber increased (P <0.01). The digestible energy and metabolizable energy content of diets decreased (P <0.01) as the total dietary fiber increased.     

营养水平对鲁农2号配套系生长肥育猪生长性能以及养分消化率的影响

为探讨鲁农2号配套系生长肥育猪适宜的能量和粗蛋白质需要量,试验采用单因子随机区组设计,在保持消化能/粗蛋白质比不变的基础上,设为高能量粗蛋白质、中能量粗蛋白质和低能量粗蛋白质3个处理组。选取健康、初始体重（49.58±1.49）kg的鲁农2号配套系生长肥育猪108头（公母各半）随机分3个处理,每个处理4个重复,每个重复（圈）9头猪。试验分为50～80 kg和80～100 kg两个阶段。结果表明,试验全期饲粮营养水平的降低对猪日采食量、日增重和料重比影响不显著,但50～80 kg低营养水平组日增重和粗蛋白质日摄入量显著低于高、中营养水平组。养分表观消化率,50～80 kg以低营养水平组最高,与中营养水平组相比,其干物质、有机物、粗蛋白质和能量的表观消化率分别提高6.97%、8.53%、10.48%和6.45%,差异显著。饲粮营养水平对钙和磷的表观消化率均无显著影响（P〉0.05）。80～100 kg不同营养水平组养分表观消化率差异显著,其中,高营养水平组养分表观消化率最高,其干物质表观消化率分别比中、低营养组提高5.49%和7.17%（P〈0.05）;有机物的表观消化率分别提高4.48%和6.07%（P〈0.05）;钙的表观消化率分别提高32.76%和27.93%（P〈0.05）;磷的表观消化率分别提高38.77%（P〈0.05）和17.44%;粗蛋白质的表观消化率比中营养水平组提高8.56%（P〈0.05）。综合考虑,在50～80 kg阶段,鲁农2号配套系生长猪适宜的消化能为12.98 MJ/kg、粗蛋白质为14.56%;80～100 kg阶段,鲁农2号配套系肥育猪适宜的消化能为12.98 MJ/kg、粗蛋白质为12.40%。     

Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition

Microbiota play an important role in total tract nutrient digestion, especially when fibrous diets are fed to pigs. This study aimed to use metagenomics to predict faecal nutrient digestibility in grower-finisher pigs. The study design consisted of 160 three-way crossbreed grower-finisher pigs (80 female and 80 male) which were either fed a diet based on corn/soybean meal or a more fibrous diet based on wheat/barley/by-products. On the day before slaughter, faecal samples were collected and used to determine faecal digestibility of dry matter, ash, organic matter, crude protein, crude fat, crude fibre and non-starch polysaccharides. The faecal samples were also sequenced for the 16S hypervariable region of bacteria (V3/V4) to profile the faecal microbiome. With these data, we calculated the between-animal variation in faecal nutrient digestibility associated with variation in the faecal microbiome, that is the “microbiability”. The microbiability values were significantly greater than zero for dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides, ranging from 0.58 to 0.93, as well as for crude fat with a value of 0.37, but not significantly different from zero for ash. Using leave-one-out cross-validation, we estimated the accuracy of predicting digestibility values of individual pigs based on their faecal microbiota composition. The accuracies of prediction for crude fat and ash digestibility were virtually 0, and for the other nutrients, the accuracies ranged from 0.42 to 0.63. In conclusion, the faecal microbiota composition gave high microbiability values for faecal digestibility of dry matter, organic matter, crude protein, crude fibre and non-starch polysaccharides. The accuracies of prediction are relatively low if the interest is in precisely predicting faecal nutrient digestibility of individual pigs, but are promising from the perspective of ranking animals in a genetic selection context.     

断奶仔猪饲喂紫花豌豆对日粮中养分的消化率和生长性能的影响

最大程度地在猪粮中用紫花豌豆代替豆粕(Soybean Meal,SBM)对生猪生产非常经济而且显得特别重要,本研究评估了在乳猪饲料中增加紫花豌豆含量替代SBM对日粮中养分的消化率和生长性能的影响。试验选取19日龄断奶后1周的乳猪260头(8.5 kg),阶段1进行2周(1~14 d),然后进行阶段2饲喂3周(15~35 d),并且用以小麦为主粮的每千克含有0,100,200,300和400 g紫花豌豆(Pisum sativum L.,subsp.hortense)的5种颗粒饲料代替含300 g/kg SBM和100 g/kg小麦饲料。两个阶段的饲料分别被设计成含有净能量(Net energy,NE)10.2和9.8 MJ/kg,同时使标准回肠可消化(Standardised Ileal Digestible,SID)赖氨酸含量为1.2和1.0 g/MJ NE。为平衡饲料中的NE,在两个饲喂阶段的日粮中将加拿大菜籽油含量分别从48和27减少到34和12 g/kg,并通过增加氨基酸结晶体平衡氨基酸含量。结果表明,当饲料中紫花豌豆含量增加到400 g/kg时,在阶段1粗蛋白(Crude Protein,CP)和总能量(Gross Energy,GE)的全消化道表观消化率(Apparent Total Tract Digestibility Coefficient,CATTD)分别被线性的降低了7%和2%(P0.001),但是在阶段2中,粗蛋白的CATTD只线性的降低了1%(P0.005)。与此同时,当饲料中紫花豌豆含量增加到400 g/kg时,日粮NE值在阶段1二次递减0.4 MJ/kg(P0.001),并在阶级2线性的减少0.2 MJ/kg(P0.001),但是用于饲料配方的紫花豌豆的NE值对于刚断奶的仔猪被高估了。在1~7 d,增加紫花豌豆的含量并不影响日均采食量(Average Daily Feed Intake,ADFI),但日增重(Average Daily Gain,ADG)和饲料转化效率(G:F)线性的减少了(P0.01);在8~14 d和15~21 d,生长性能并没有受到影响;在22~28 d,增加紫花豌豆的含量使ADFI和ADG二次递增(P0.01),但没有影响G:F;在29~35 d,增加紫花豌豆的含量时,ADFI只是趋向性线性增加(P0.10),但ADG却表现出线性增加(P0.05),同时没有影响G:F。在整个试验过程(1~35 d),增加饲料中紫花豌豆的含量并没有影响ADFI,ADG和G:F。因此得出结论,对于乳猪饲料,在配成等量的NE值和SID氨基酸后,400 g/kg紫花豌豆能够完全替代SBM,并且对断奶后经过7d的适应期的仔猪的生长性能没有不利影响。     

The effects of dietary fiber level on nutrient digestibility in growing pigs

The objective of this study was to investigate the effects of total dietary fiber level on nutrient digestibility and the relationship between apparent total tract digestibility of total dietary fiber, and soluble dietary fiber, insoluble dietary fiber and available energy. Sugar beet pulp was as the only fiber source. The experiment was designed as a 6 x 6 Latin square with an adaptation period of 7 d followed by a 5-d total collection of feces and urine. Feed intake tended to decrease （P =0.] O） as total dietary fiber level increased. The apparent total tract digestibility of dry matter, crude protein and gross energy decreased （P 〈0.0]） when total dietary fiber increased but the digestibility of soluble dietary fiber and insoluble dietary fiber increased （P 〈0.01）. The digestible energy and metabolizable energy content of diets decreased （P 〈0.01） as the total dietary fiber increased.     

Hematologic and biochemical reference intervals for Norwegian crossbreed grower pigs

Background: Hematologic and biochemical reference intervals depend on many factors, including environment and age. Reference intervals for Norwegian grower pigs are lacking, and previously published reference intervals for similar pigs from other countries are now outdated due to significant changes in management and breeding on the pig farms. Objectives: The aim of this study was to determine updated reference intervals for hematologic and biochemical analytes in healthy crossbred grower pigs, and to compare the results among 3 different farms. Methods: Blood samples were collected from 104 clinically healthy pigs of the most common Norwegian crossbreed (Landrace Yorkshire sow × Landrace Duroc boar). The pigs were 12–16 weeks old, weighed 30–50 kg, of both sexes, and lived on 3 farms in eastern Norway. Automated hematologic and biochemical analysis were performed using ADVIA 2120 and ADVIA 1650 analyzers. Results: Five samples were excluded because of hemolysis (1) or outliers (4). Reference intervals were calculated using parametric or nonparametric methods, depending on data distribution. Mean, median, minimum, and maximum values were tabulated. Conclusions: The reference intervals calculated in this study will be useful for the diagnosis and monitoring of disease in this widespread crossbreed pig. Compared with previously published reference values, reference intervals for total WBC count, creatine kinase and alanine aminotransferase activities, and albumin, bilirubin, and urea concentrations in this study differed notably.     

Dietary galactooligosaccharides affect ileal and total-tract nutrient digestibility, ileal and fecal bacterial concentrations, and ileal fermentative characteristics of growing pigs

The objective of this study was to evaluate dietary galactooligosaccharide (Gal OS) addition on swine nutrient digestibility, ileal and fecal bacterial populations, and ileal short-chain fatty acid (SCFA) production, and to determine their impact on ileal fermentative characteristics in vitro. Twelve T-cannulated pigs (BW = 25 kg) were fed a diet free of Gal OS for 21 d. On d 22, ileal digesta samples were collected for an in vitro fermentation experiment (Exp. 1). Substrates included: raffinose/stachyose combination (R + S), soy solubles (SS), and transgalactooligosaccharides (TOS). Also included were the non-OS components of SS and TOS. Nine pigs (three donors per treatment) served as ileal effluent donors. Each substrate was fermented in vitro for 6 h, and pH and SCFA and gas production were determined. Pigs then were allotted to three treatments: a Gal OS-free control diet and the control diet with either 3.5% added Gal OS from SS or TOS. Diets, feces, and digesta samples collected weekly for 6 wk on d 6 (feces) and 7 (digesta) were analyzed for DM, OM, CP, and chromic oxide concentrations. Feces and ileal digesta were analyzed for bifidobacteria and lactobacilli populations. Ileal digesta samples were analyzed for SCFA. On d 64, a second in vitro fermentation experiment (Exp. 2) was conducted using ileal effluent from three pigs per treatment and the same substrates used in Exp. 1. In vivo results showed that ileal and total tract DM and OM digestion were decreased (P < 0.05) by addition of both SS and TOS to the diet. Ileal and total-tract N digestibilities were decreased (P < 0.05) by dietary addition of SS. Fecal bifidobacteria and lactobacilli were increased (P < 0.05) by addition of SS and TOS to the diet. Ileal propionate and butyrate concentrations were greater (P < 0.05) for pigs fed diets containing both sources of Gal OS. In vitro results showed that fermentation data were not affected by donor animal adaptation to treatment. For both in vitro experiments, gas and SCFA production were higher (P < 0.05) for R + S than for SS or TOS. Fermentation of R + S resulted in a higher pH (P < 0.05) than did SS or TOS. Fermentation of non-OS components of SS and TOS resulted in more (P < 0.05) gas and SCFA production, and pH values that did not differ (P > 0.05) compared to SS and TOS. The Gal OS used in this study were prebiotics, increasing beneficial bacteria in vivo and SCFA concentrations both in vivo and in vitro.     

Ileal digestibility of protein and amino acids from canola meal in weaned piglets and growing pigs

Two experiments were conducted to measure the apparent ileal digestibility coefficients (AID) of protein and amino acids from canola meal (CM) and a pelleted canola meal (PCM) and their effect on specific activity (SA) of pancreatic proteases in weaned piglets and growing pigs. In experiment one, 24 piglets weaned at 17 days and weighing 5.5 kg were used. Treatments were a reference diet with 200 g of crude protein (CP) kg^− 1 elaborated with casein (C) as the sole protein source, a diet containing C–CM and a diet containing C–PCM. These diets were obtained using the reference diet plus 100 g kg^− 1 of CM or PCM that substituted an isoproteic mixture of casein and maize starch from reference diet, so that the AID coefficients for their protein and amino acids could be calculated by difference. In experiment two, nine castrated pigs weighing 39.5 kg were used. Treatments were a reference diet with 160 g of CP kg^− 1 elaborated with casein (C) as the sole protein source, diet C–CM and diet C–PCM. These diets were obtained using the reference diet plus 300 g kg^− 1 of CM or PCM that substituted an isoproteic mixture of casein and maize starch from reference diet. In piglets, the AID coefficients for casein were highest (P < 0.05), those of PCM were intermediate, and those of CM were the lowest. In older pigs, the AID coefficients for casein were highest, and those of CM and PCM were similar (P > 0.05). The SAs of chymotrypsin, trypsin and carboxypeptidases A and B were lower in piglets than in heavier pigs. Moreover, the SAs of trypsin, chymotrypsin, and carboxypeptidase B were lower (P > 0.05) in animals fed casein. The results showed in piglets that whereas CM was less digestible, pelleted canola meal improved protein and amino acid ileal digestibility, resulting in similar AID coefficients to those of growing pigs.     

Ileal digestibility of nutrients and amino acids in unfermented,fermented soybean meal and canola meal for weaning pigs

Apparent ileal digestibility (AID) of energy, dry matter, nitrogen and amino acids and standardized ileal digestibility (SID) of nitrogen and amino acids were evaluated in six weanling pigs ((Landrace × Yorkshire) × Duroc)) fed unfermented soybean meal (SM), yeast fermented soybean meal (SMY), bacillus fermented soybean meal (SMB), yeast and bacillus fermented soybean meal (SMYB), canola meal (CM) and nitrogen‐free diet. Pigs having body weights 17.00 ± 0.3 kg were surgically equipped with T‐cannulas of approximately 15 cm prior to the ileo‐cecal junction and randomly allotted to one of five dietary treatments and a nitrogen‐free diet in 6 × 6 Latin squares. AID and SID of nitrogen (N) was greater (P < 0.05) in SMYB and SMB compared with SM and CM. AID and SID of amino acids such as, Lys (lysine) and Phe (phenylalanine) as well as total essential amino acids were greater (P < 0.05) in SMB and tended to be low in CM compared with SM. AID and SID of aspartic acid (Asp) and glycine (Gly) tended to be higher in SMB compared with SM and other diets except CM. In conclusion, fermentation of soybean meal by Bacillus showed better digestibility of amino acid and nutrients.     

Digestible lysine requirement of starter and grower pigs

Three experiments were conducted to determine the digestible lysine requirement of starter (6 kg BW initially) and of grower (21 kg BW initially) pigs. Experiment 1 used 294 starter pigs and lasted 28 d; Exp. 2 used 182 grower pigs and lasted 35 d. Protein and total lysine contents of the basal corn-peanut meal diets were 20 and .8% for Exp. 1 and 16 and .54% for Exp. 2. Basal diets were fortified with five incremental additions of lysine.HCl to provide lysine contents ranging from .8 to 1.3% in Exp. 1, and .54 to .94% in Exp. 2. Diets contained crystalline tryptophan, threonine and isoleucine (Exp. 1 only) to provide dietary concentrations equal to 18, 70 and 60% of the highest lysine level fed. Average daily gain and gain/feed of both starter and grower pigs increased (P less than .05) linearly and quadratically as dietary lysine level increased. For starter pigs, ADG and gain/feed were optimized at 1.1 to 1.2% total lysine. For grower pigs, ADG and gain/feed were optimized at .86% total lysine. In Exp. 3, barrows fitted with an ileal T-cannula were used in a 4 X 4 Latin square design. Basal diets and diets with added lysine were evaluated. Apparent lysine digestibility of the basal starter and grower diets and lysine.HCl were 79.9, 74.1 and 96.7%, respectively. Based on these values and the total lysine contents found to optimize performance, the digestible lysine requirements of starter and grower pigs are 1.03 and .71%, respectively.     

Dietary protein level affects nutrient digestibility and ileal microbiota structure in growing pigs

This study aimed to determine whether dietary protein content influences pig health as indicated by ileal microbiota structure and coefficients of total tract apparent digestibility (CTTAD) of nutrients. Seventy‐two gilts, with an initial body weight of 29.9 ± 1.5 kg, were used in this 42‐day feeding study. Pigs were randomly allotted to one of three dietary treatments of corn‐soybean meal contained 14, 16 or 18% crude protein (CP). As dietary CP content decreased, the CTTAD of most essential amino acids (AAs), except for arginine and histidine, increased linearly, while those of most nonessential AAs decreased linearly. The concentration of total short‐chain fatty acids (SCFA) was higher in pigs fed the diet with 14% CP content than others. Ileal microbiota structure was changed by dietary treatments. In particular, at the phylum level, the relative abundance of Tenericutes in ileal digesta decreased as the dietary protein content reduced, while that of cyanobacteria increased. At the genus level, the relative abundance of Weeksella, Phaseolus acutifolius, Slackia, Sulfurimonas and Aerococcus showed significant differences among the three dietary treatments. In conclusion, ileal microbiota structure was changed by dietary protein content. Moderate reduction of protein intake can benefit gut health by enhancing the gut microbial fermentation and SCFA formation.     

日粮中添加发酵饲料对育肥猪养分消化率及肠道菌群的影响

为研究日粮中添加不同水平的发酵饲料对育肥猪养分消化率及肠道菌群的影响,试验选用560头杜×长×大三元育肥猪[(75.37±2.65)kg],根据体重、性别随机分为7组,每组5个重复,每个重复16头,对照组饲喂育肥猪日粮,试验组分别在育肥猪日粮中添加5%或10%的发酵饲料A、B、C,饲喂21 d后每组选5头育肥猪肛门采集新鲜粪便测定肠道菌群多样性;并在饲喂第29天和第30天,各组每个重复连续收集粪便混合后测定养分消化率。结果表明:日粮中添加发酵饲料对育肥猪粗蛋白质、能量和干物质的消化率没有显著影响(P>0.05),相比对照组,饲喂发酵饲料组的粗蛋白质消化率有所提高(最高组提高2.9%);同时肠道菌群中乳杆菌属丰度有所增加,表明在日粮中添加一定比例的发酵饲料可以改善育肥猪养分消化率和肠道健康。     

Effect of group size and feeding regimen on nutrient digestibility studies with weanling pigs

An experiment was conducted to determine the effects of feeding regimen and group size on nutrient (N, Lipid, Ca, P) balance measurements with weanling swine. Treatments imposed were 1) one pig pair-fed to 90% of treatment 3, 2) three pigs pair-fed to 90% of treatment 3 and 3) three pigs with ad libitum access to their diet. A total of 28 barrows weaned at 21 d of age (initial BW 5.9 kg) were allotted by weight and litter in a randomized complete block design with repeated measurements over time to the three treatments in four replicates. The pigs were placed in .6-m x 1.13-m stainless steel metabolism crates and fed the same experimental diet for an initial 4-d adjustment period. This was followed by a 5-d collection of urine and feces with a 2-d interval between collection periods each week of a 4-wk postweaning period. When feed was restricted, N intake, urine N, fecal N and retained N were similar whether pigs were fed as singles or in groups of three. Individually fed pigs had a higher urinary Ca but lower urinary P compared with those fed in groups of three. Lipid and P retention values were similar for individual and group-penned, restricted-fed animals. Pigs provided ad libitum access to feed had greater weight gains and nutrient intakes, excretions (urine, fecal) and retentions (P less than .01) than pigs that were limit-fed. Similar responses among treatments occurred each week of the 4-wk trial. These results suggest that effective weekly balance data can be collected each week sequentially with 21-d weanling pigs penned in groups and allowed to consume feed ad libitum, a situation more similar to natural nursery conditions.     

不同替代比例肠膜蛋白粉对猪有效能及营养物质消化率的影响

试验旨在通过套算法研究不同替代比例的肠膜蛋白粉对猪的有效能和营养物质表观消化率的影响。试验将24头平均体重(20.2±2.3) kg的健康去势公猪随机分为4组,每组6个重复。对照组饲喂玉米基础日粮,试验组分别是10%、20%和30%的肠膜蛋白粉替代基础日粮的试验日粮。试验期12 d,前7 d为预饲期,后5 d为粪尿收集期。结果显示,以干物质为基础,以10%、20%和30%肠膜蛋白粉替代基础日粮,试验得出的肠膜蛋白的表观代谢能分别为12.01、12.52 MJ/kg和12.10 MJ/kg(P<0.05);随着替代水平的升高,总能、有机物和中性洗涤纤维(P<0.05)的消化率降低,粗蛋白消化率先升高再降低。由此可见,生长猪对不同替代比例的各营养物质的表观消化率具有一定差异性,随着肠膜蛋白粉在基础日粮中替代比例的提高,肠膜蛋白粉的有效能值先升高再降低;当肠膜蛋白粉的比例是20%时,其原料的消化能和代谢能相对较高。     

发酵木薯酒精糟对生产猪生产性能及营养物质消化的影响

试验研究不同水平的发酵木薯酒精糟对生长猪生产性能的影响。选择200头初始体重约(26.35±0.43)kg的生长猪,随机分为5个处理组,每组4个重复,每个重复10头猪。5个处理组分别为:A组为对照组,饲喂基础日粮;B组、C组和D组分别为发酵木薯酒精糟用量为2%、6%和15%的配合日粮组,E组则为用发酵木薯酒精糟直接替代6%基础日粮的混合日粮组。结果表明,B组和C组的日增重较A组分别提高了6.45%(P0.05)和4.84%(P0.05),各试验组料肉比均低于对照组,分别降低了7.48%、9.06%、5.12%和0.39%。日粮中添加发酵木薯酒精糟后有效改善了营养物质消化率,B组、C组、D组及E组干物质消化率较对照组分别提高了8.62%(P0.05)、7.50%(P0.05)、4.75%(P0.05)和5.26%(P0.05);粗蛋白消化率较对照组分别提高了11.43%(P0.05)、9.50%(P0.05)、9.61%(P0.05)和10.61%(P0.05);粗脂肪消化率较对照组分别提高了15.66%(P0.05)、15.86%(P0.05)、12.98%(P0.05)和14.10%(P0.05);钙的消化率则较对照组分别提高了10.46%(P0.05)、6.50%(P0.05)、6.93%(P0.05)和10.60%(P0.05)。B组、C组、D组及E组较A组的养殖效益均有所提高,每千克饲料的增重成本分别降低了0.683、0.833、0.523元和0.238元,其中使用处理C组饲料的效益最好。本试验结果说明,生长猪日粮中发酵木薯酒精糟的适宜添加量为2%~6%,能有效提高猪的生产性能和养殖效益。     

Glycemic index of starch affects nitrogen retention in grower pigs

Three studies were performed to examine the effect of starch and protein digestion rates on N retention in grower pigs. In Exp. 1, the glycemic index (GI) of corn, a malting barley, and a slow-rumen-degradable barley (SRD-barley) were measured using 6 barrows (BW = 18.0 ± 0.5 kg). The GI of malting barley was greater (P < 0.05) than that of SRD-barley (71.1 vs. 49.4), and the GI of both barley cultivars was less (P < 0.05) than that of corn (104.8). In Exp. 2, the standardized ileal digestibility of AA and DE content of the 3 ingredients were determined using 5 ileal-cannulated barrows (BW = 20.7 ± 2.3). The apparent total-tract energy digestibility values of corn (86.1%) and malting barley (85.7%) were greater (P < 0.05) than that of SRD-barley (82.3%). The standardized ileal digestibility of Lys was 94.0, 92.6, and 92.4% for corn, malting barley, and SRD-barley, respectively, and did not differ among grains. In Exp. 3, 6 diets were formulated to equal DE (3.40 Mcal/kg), standardized ileal digestibility of Lys (8.6 g/kg), starch (424.9 g/kg), and digestible CP (180.0 g/kg) using the values obtained in Exp. 2. Three GI [high (corn), medium (malting barley), and low (SRD-barley)] and 2 rates of protein digestion [rapid (soy protein hydrolysate) and slow (soy protein isolate)] were tested in a 3 × 2 factorial arrangement with 36 barrows (BW = 32.2 ± 2.5 kg). Pigs were fed 3.0 times the maintenance energy requirement daily in 2 meals for 2 wk and were housed in metabolic crates to collect feces and urine separately. At the end of the study, intestinal contents were collected from 4 equal-length segments of the small intestine. The percentage of unabsorbed CP in segment 1 relative to dietary CP was greater (P < 0.05) for the soy protein isolate diet than for the soy protein hydrolysate diet (170.3 vs. 116.5%). The percentages of unabsorbed starch in segments 1 and 2 were greater (P < 0.05) for the SRD-barley diet than for the malting barley or corn diet. Nitrogen intake and fecal N excretion were greater (P < 0.05) for pigs fed the malting barley and SRD-barley diets than for pigs fed the corn diet. Urinary N excretion was greater (P < 0.05) for pigs fed the SRD-barley diet than for pigs fed the corn or malting barley diet. Pigs fed slowly digestible starch (SRD-barley; 46.6%) had less (P < 0.05) net N retention than pigs fed corn or malting barley (54.7 and 54.1%, respectively). In conclusion, slowly digestible starch sources such as SRD-barley may not be suitable to support maximum protein deposition in restricted-fed grower pigs.