共查询到20条相似文献,搜索用时 15 毫秒
1.
Fernández-Silva JA Abdulmawjood A Akineden O Bülte M 《Tropical animal health and production》2011,43(8):1501-1507
The objective of this study is the detection of Mycobacterium avium subsp. paratuberculosis (MAP) by serum enzyme-linked immunosorbent assay (ELISA), fecal polymerase chain reaction (PCR), and fecal culture in Colombian
dairy herds. Serum and fecal samples from asymptomatic cows (n = 307) of 14 dairy herds were tested for MAP by an unabsorbed ELISA test (ELISA-A). Serum and fecal samples from positive
ELISA-A animals (n = 31) were further tested by an absorbed ELISA test (ELISA-B) and PCR. Fecal samples from animals of herds positive by ELISA-A
and PCR (n = 105) were inoculated onto three different culture media. ELISA-A produced positive results in 10% of the serum samples
and 71% of the herds. ELISA-B and PCR results were positive in two and six serum and fecal samples from positive ELISA-A animals,
respectively. Fecal samples were negative for MAP on all culture media. The results of this study confirmed the presence of
MAP in local dairy herds and the difficulties of MAP detection in asymptomatic animals by ELISA, PCR, and fecal culture. 相似文献
2.
Mehdi Zarei Masoud Ghorbanpour Samaneh Tajbakhsh Nader Mosavari 《Tropical animal health and production》2017,49(6):1195-1200
Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of MAP in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne’s disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of MAP in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne’s disease status. ELISA was performed to determine the MAP antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that MAP can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne’s disease have limited value in detecting cattle with MAP infection in liver. These findings show that the presence of MAP in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to MAP. Given concerns about a potential zoonotic role for MAP, these results show the necessity to find new methods for detecting cattle with MAP disseminated infection. 相似文献
3.
M. P. Soumya R. M. Pillai P. X. Antony H. K. Mukhopadhyay V. N. Rao 《Veterinary research communications》2009,33(7):781-791
Comparative efficacy of faecal culture and IS900 Polymerase chain reaction (PCR) assay of faecal samples was investigated
in 40 clinically suspected cases of Johne’s disease in dairy cattle. The sensitivity of faecal culture and PCR assay in this
study was 52.5% (21/40) and 90% (36/40) respectively. All isolates appeared only on the mycobactin J supplemented Herrold’s
egg yolk medium (HEYM) at 8–16 weeks post-inoculation, were acid-fast and were positive for IS900 PCR yielding a single amplicon
of 217 bp. A total of 28 faecal samples out of 40 were positive by IS900 primary PCR assay for Mycobacterium avium subsp. paratuberculosis (Map) yielding an expected product of size 217 bp. Twelve faecal samples, which gave negative results in the primary PCR,
were subjected to secondary PCR assay. Of the 12 samples, 8 gave positive results in the IS900 nested PCR (nPCR), which yielded
a PCR product of 167 bp, proving better sensitivity of nPCR assay than single amplification PCR. PCR could detect additionally
15 samples as positive which were negative by faecal culture. The chi-square analysis showed a highly significant difference
between the tests (P< 0.01). This study suggests that IS900-PCR-based detection of Map could be used as a potential diagnostic
tool for rapid and effective Johne’s disease surveillance. 相似文献
4.
Ansari-Lari M Haghkhah M Bahramy A Novin Baheran AM 《Tropical animal health and production》2009,41(4):553-557
A cross-sectional study was conducted from March to August 2006 in dairy herds in Fars province, southern Iran to determine
the herd-level risk factors for infection with Mycobacterium avium subspecies paratuberculosis (MAP). Statistical analysis using multivariable logistic regression showed that contamination of udders of periparturient
cows with manure (OR = 6.4, P = 0.02) and history of having suspected cases of Johne's disease in the herd (OR = 6.7, P = 0.04)
were significantly associated with the herd infection status. No relationship between breed, herd size and other management
practices with the infection status of the herd were found in this study. Implementing high sanitary measures in the farm,
particularly with respect to manure handling and cleaning could be considered as one of the important aspects in controlling
disease in the region as well as in the future educational effort. 相似文献
5.
Maddur MS Mohan MS Gajendragad MR Gopalakrishna S Singh N 《Veterinary research communications》2008,32(6):481-489
Foot-and-mouth disease (FMD) is one of the most contagious diseases affecting wide range of host species with variable severity and decreased productivity. The present study was undertaken to compare the clinical and leucocytic changes in indigenous Indian cattle and buffaloes experimentally infected with FMD virus (FMDV) Asia 1. A mild type of disease was observed in the cattle, more so in buffaloes infected with FMDV. Difference in terms of type, site and healing of lesion was observed between cattle and buffaloes. Foot lesions were more common than tongue in buffaloes, which were mainly evident in bulb of the heel in contrast to interdigital foot lesions in cattle. Further, FMDV infection induced a transient moderate leucopenia with lymphopenia in both cattle and buffaloes, but monocyte levels diverged. Relationship between the raised body temperature, leucocytic changes and lesion development was observed. Microscopic changes were observed in the keratinized epithelium of tongue and foot. The findings of the present study indicated the need to investigate the early leucocytic changes in cattle and buffaloes in depth for better understanding of the disease process. 相似文献
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Camacho AT Guitian FJ Pallas E Gestal JJ Olmeda AS Habela MA Telford SR Spielman A 《Tropical animal health and production》2005,37(4):293-302
The control of equine piroplasmosis is becoming increasingly important to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Theileria equi and Babesia caballi) in Galicia, north-west Spain, and to compare haematological and serum biochemistry parameters between non-parasitaemic horses and horses parasitaemic with T. equi and B. caballi. Sixty serum samples (control group) were taken from healthy horses pastured on two farms, and examined for evidence of equine T. equi and B. caballi infection by indirect fluorescent antibody test (IFAT). Of the 60 samples, 24 (40%) and 17 (28.3%) samples were positive for T. equi and B. caballi, respectively. Twelve (20%) samples were positive for both parasites. Haematology and serum biochemistry were compared between controls and a series of 36 horses clinically affected by T. equi (25) or B. caballi (11). Compared with the healthy group, there was a 43% and 37% decrease in the haematocrit for T. equi and B. caballi infection, respectively. Parasitaemic horses presented an intense anaemia and serum biochemistry signs of liver damage. The anaemia was more severe in T. equi-infected than in B. caballi-infected horses. Our results suggest that equine piroplasmosis is widespread in the region and is a cause for concern. 相似文献
8.
Simeon I. B. Cadmus Mohammed K. Yakubu Abdullahi A. Magaji Akinbowale O. Jenkins Dick van Soolingen 《Tropical animal health and production》2010,42(6):1047-1048
Using deletion typing technique, five mycobacteria isolated from unpasteurised milk samples from cows in north-central Nigeria
were characterized as Mycobacterium bovis (n = 4) and M. africanum (n = 1). This report emphasizes that transmission between the animal and human reservoir is a serious threat in Nigeria. 相似文献
9.
Hossein Nourani Somayeh Matin Abass Nouri Hamidreza Azizi 《Tropical animal health and production》2010,42(6):1225-1227
Bovine sarcocystosis is caused by Sarcocystis cruzi and is known to cause considerable morbidity and mortality in cattle. This species is distributed worldwide in cattle and
is the most prevalent of the Sarcocystis species infecting cattle. There is high infection rate of sarcocyst in cattle in Iran, but to our knowledge, there is no
study about identification of Sarcocystis species. This work aimed to survey prevalence of S. cruzi cyst in slaughtered cattle of Isfahan, Iran. In this study, esophageal and diaphragmatic muscles of 100 cattle were collected
from Fesaran abattoir of Isfahan and examined for the presence of Sarcocystis spp. cysts macroscopically and microscopically. No macroscopic sarcocysts were found in any of the samples. In light microscopy,
89 out of 100 cattle (89%) had thin-walled cysts of S. cruzi, while 21 out of them (21%) had thick-walled sarcocysts. In addition to light microscopy, ultrastructural features of the
thin-walled cyst confirmed the presence of S. cruzi. 相似文献
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Maria Gabriela Xavier Oliveira Leandro Nogueira Pressinotti Giovane Spínola Carvalho Mirela Caroline Vilela Oliveira Luisa Zanolli Moreno Carlos Emilio Cabrera Matajira Alessandro Spínola Bergamo Victor Manuel Aleixo Alexandre Caixeta Veiga Elvis de Souza Corsino Ana Paula Guarnieri Christ Maria Inês Zanolli Sato Andrea Micke Moreno Terezinha Knöbl 《Tropical animal health and production》2017,49(4):777-782
The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans. 相似文献
14.
Ozlem Ozmen Mehmet Kale Mehmet Haligur Sibel Yavru 《Tropical animal health and production》2009,41(6):951-958
In this study, pathological, serological and virological examinations were performed on 15 sheep from a flock of 250 sheep
and lambs that suffer from simultaneous naturally occurring BTV, PPRV and SPV outbreaks. SPV was diagnosed macroscopically
and histopathologically, BTV was diagnosed by ELISA, and PPRV was diagnosed pathologically and by ELISA. Clinically fever,
diarrhea, depression, polypnea, conjunctivitis, lacrimation, rhinitis, erosive stomatitis, edema of eyelids, photophobia,
cutaneous eruption with erythematous areas especially noticeable in wool-free parts of the body and axilla lesions evolving
into papules were observed. At necropsy, the most effected organs were lungs and gut. Subepicardial hemorrhages were also
commonly seen. While typical pox lesions were observed in some lambs, usually fibrinous pleuropneumonia was more prominent
lung lesion. SPV and PPRV lesions were seen at the histopathological examination of the lesioned tissues, BT lesions were
mild than SPV and PPRV microscopically. Serum and leukocyte samples of 15 animals were examined for PPRV and BTV by ELISA;
5 samples were positive for PPRV and 6 BTV, 4 were positive for both PPRV and BTV simultaneously. One hundred animals died,
most were lambs. Mortality rates were 100% in lambs and 80% in the herd. 相似文献
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Amanda Bonalume Cordeiro de Morais Carmen Alicia Daza Bolaños Ana Carolina Alves Cássia Yumi Ikuta Gustavo Henrique Batista Lara Marcos Bryan Heinemann Rogério Giuffrida Fernando Paganini Listoni Mateus de Souza Ribeiro Mioni Rodrigo Garcia Motta Shinji Takai Márcio Garcia Ribeiro 《Tropical animal health and production》2018,50(6):1319-1326
17.
Ferreira SR de Araújo JV Braga FR Araujo JM Frassy LN Ferreira AS 《Veterinary research communications》2011,35(8):553-558
Ascaris suum is a gastrointestinal nematode parasite of swines. The aim of this study was to observe Pochonia chlamydosporia fungus on biological control of A. suum eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided
into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3
did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing
P. chlamydosporia (VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h
after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000 A. suum eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately
100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity
parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration
and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (p > 0.01). P. chlamydosporia remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying A. suum eggs. 相似文献
18.
Hao Wang Zihua Li Fu Gao Jiaqing Zhao Mingxing Zhu Xin He Nan Niu Wei Zhao 《Veterinary research communications》2016,40(2):73-79
Objective
This study aims to investigate the immunoprotection of recombinant Eg.P29 (rEg.P29) vaccine and analyze the underlying mechanism in sheep.Methods
Three groups of male sheep were immunized subcutaneously with rEg.P29 and PBS, Freund’s complete adjuvant as controls, respectively. After prime-boost vaccination, the sheep were challenged with encapsulated Echinococcus granulosus eggs. The percentage of protection in sheep was determined 36 weeks after the infection. Humoral immune response was analyzed for specific IgG, IgG1, IgG2, IgM and IgE levels. Moreover, cytokines including interferon (IFN)-γ, interleukin (IL)-2, IL-4,and IL-10 were also evaluated.Results
Immunization with rEg.P29 induced protective immune responses up to 94.5 %, compared with immunoadjuvant group. The levels of specific IgG, IgG1, IgG2, and IgE as well as IFN-γ, IL-2, and IL-4 significantly increased after two immunizations (P < 0.05); however, the levels of IgM and IL-10 did not show difference.Conclusion
rEg.P29 showed Immunoprotection and induced Th1 and Th2 immune responses; hence, rEg.P29 is a potential vaccine for E. granulosus infection.19.
Benjamin Obukowho Emikpe Mohammed Y. Sabri Stephen O. Akpavie Mohammed Zamri-Saad 《Veterinary research communications》2010,34(7):569-578
Nigerian strain of Peste des Petit Ruminant (PPR) virus and Mannheimia haemolytica (MH) biotype A serotype 2, was used successfully to reproduce a concurrent disease in West African Dwarf goats. The development
of the various pathological features were studied at regular intervals following infection. The acute inflammatory reaction
which had developed by day 3 after initial infection was characterised by flooding of the alveoli by neutrophils, oedema,
hemorrhage and syncytial cells together with a moderate bronchial and bronchiolar epithelial necrosis. This progressed to
a milder acute broncho interstitial pneumonia with giant cells. At this stage, the mucosal immunity were well developed especially
the aggregate form of NALT and more of nodular forms of BALT. The organisms were demonstrated with strong immunostaining in
the necrotic center, necrotic alveolar wall, fibrin, serous exudate, and degenerated leukocyte in the alveoli and respiratory
airways. The bacterial antigens were observed as a strong immunostaining in the blood vessels of the nasal septum, sinusoid
in the liver and interstium of the kidney, cytoplasm of alveolar macrophages, pneumocytes, bronchial and bronchiolar epithelium,
in the monocytes in the blood vessels. These findings confirmed the enhancement of MH tropism especially in the respiratory
tract, liver and kidney. It also showed that West african dwarf goats are highly susceptible to the intratracheal combined
infection of PPR virus and MH. The fact that the infection induces strong mucosal responses, this phenomenon can be explored
in Africa with the use of combined PPR virus and MH intranasal vaccines to curtail the menace of pneumonia associated with
the combined infection on field. 相似文献
20.
Thallyta Maria Vieira Leydiana Duarte Fonseca Gabriela Almeida Bastos Viviane de Oliveira Vasconcelos Maria Luíza França Silva Franciellen Morais-Costa Adriano Vinícius de Paiva Ferreira Neide Judith Faria de Oliveira Eduardo Robson Duarte 《Veterinary research communications》2017,41(2):99-106