ATP loss with exercise in muscle fibres of the gluteus medius of the thoroughbred horse

Muscle ATP loss with exercise has implications both to the causes of fatigue and muscle damage. To study this at the single muscle fibre level, five trained thoroughbred horses performed consecutive 90 second gallops on an inclined treadmill followed by a final gallop to fatigue. Biopsies of the m. gluteus medius were taken at rest, post-exercise and during 24 hour recovery. Blood lactate was 20·0 mmol litre⁻¹ or more, and plasma NH₃ 300–800 μmol litre⁻¹, following the final gallop. Minimal changes occurred in the plasma markers, and . ATP loss with exercise was 32·2 ( 12·2) per cent. Following exercise single fibre contents showed a much broader distribution than at rest, with contents in some close to zero. Following five and 24 hour recovery, however, frequency distribution curves were close to those seen at rest. There was no difference in the ATP contents of types I, IIa and IIb at rest of with exercise or recovery. The results pointed to marked heterogeneity between individual fibres in their biochemical response with exercise, independent of fibre type.     

Effect of dietary chloride content on the elimination of bromide by dogs

The effect of dietary chloride content (0·2, 0·4 and 1·3 per cent chloride on a dry matter basis) on the disposition of a single oral dose of bromide (14 mg kg⁻¹ was evaluated in normal beagles. Increasing the dietary chloride content from 0·2 to 1·3 per cent resulted in a significant decrease in the mean apparent elimination half-life from 69 ± 22 days to 24 ± 7 days. The mean area under the concentration curve ( ) for dogs fed 1·3 per cent chloride was significantly smaller than the for dogs fed 0·2 per cent chloride. Dietary chloride had no effect on the maximum serum concentrations (C_max) or on the time (T_max) to reach the maximum concentrations. The steady-state serum bromide concentrations predicted from the single dose data for daily doses of 14 mg kg⁻¹ of bromide were significantly lower in dogs fed 1·3 per cent chloride (310 ± 150 mg litre⁻¹) than in dogs fed 0·2 per cent chloride (1950 ± 1140 mg litre⁻¹). The predicted mean daily doses of bromide necessary to maintain serum levels within the therapeutic range for dogs fed 1·3 per cent chloride (43 ± 13 mg kg⁻¹) were almost twice as high as the dose estimated for dogs fed 0·4 per cent chloride (22 ± 3 mg kg⁻¹) and nearly three times as high as the dose estimated for dogs fed 0·2 per cent chloride (15 ± 4 mg kg^−l). These differences were statistically significant (P=0·002).     

A comparative evaluation of the accusport VS. conventional assay methods for the determination of lactate in equine plasma

Plasma lactate concentrations were studied in 17 (11 Standardbred and six Thoroughbred), healthy racehorses following a standardized exercise test on a high-speed treadmill. Blood samples were collected from the jugular vein and placed into tubes containing lithium heparin at intervals throughout the test The blood was immediately centrifuged for 10 minutes at 3000 rpm and the plasma removed. Plasma lactate concentrations were measured using the Accusport and a commercial spectrophotometric method. Prepared lactate standards in the ranges 5 to 45 mmol. 1⁻¹ were also tested using these methods. Samples with lactate concentrations greater than 10 mmol. 1⁻¹ were diluted with double de-ionized water such that the estimated lactate concentration did not exceed 10 mmol. 1⁻¹. Accusport correlated highly with the wet chemistry method at concentrations less than 13 mmol. 1⁻¹ (r=0.983, P<0.001) for undiluted plasma and 20 mmol. 1⁻¹ (r=0.988, P <0.001) for prepared standards. Accusport underestimated lactate concentrations for plasma samples greater than 13 mmol. 1⁻¹ (r=0.313, P>0.05). Accusport continued to correlate significantly with the wet chemistry method, for prepared standards, at lactate concentrations greater than 20 mmol. 1⁻¹ (r=0.940, P<0.01), however the line of fit was significantly different from the line of unity indicating a lack of accuracy for the Accusport at lactate concentrations greater than 20 mmol. 1⁻¹. Diluted lactate samples correlated significantly with the wet chemistry method at the P<0.05 level (r=0.625), however the line of fit was significantly different from the line of unity at the P<0.1 level.     

Continuous and non-invasive study of brain oxygenation in the calf by near infrared spectroscopy

Near infrared spectroscopy (NIBS) was used to detect changes in brain oxygenation in five tranquilised calves that were placed on a hypoxic gas mixture (10 per cent O₂/90 per cent N₂) and hyperoxic gas mixture (30 per cent O₂/70 per cent N₂) for five minutes at each concentration. A NIRO 500 (Hamamatsu, Japan) was used for the NIBS, with the incident light source and separate detector (optodes) placed on shaved skin on the most dorsal surface of the frontal bone. Sequential arterial blood gas sample analyses provided confirmation of the appropriate change in systemic oxygenation status. By the end of the five-minute-period of breathing 10 per cent oxygen, NIBS of the calf head detected highly significant changes in haemoglobin oxygenation reflective of hypoxaemia, with oxyhaemoglobin decreasing by 23·5 units (P<0·01) and deoxyhaemoglobin increasing by 45·6 units, (P<0·01) from the baseline of breathing room air. Total haemoglobin (oxyhaemoglobin + deoxyhaemoglobin) showed a significant increase of 22·1 units (P<0·05) but there was no significant change in LAIRS determined cytochrome aa₃ oxygenation. Concomitant blood gas alterations included significant decreases in Pa)₂ (−27·8 mmHg, P<0·01), haemoglobin saturation (−29·0 per cent, P<0·05), and PaCO₂ (−7·8 mmHg, P<0·05) and significantly increased blood pH (0·059, P<0·05). At the end of the five minutes of breathing 30 per cent oxygen NIBS of the calf head detected significantly increased oxyhaemoglobin (13·1 units, P<0·01) and decreased deoxyhaemoglobin (−13·7 units, P<0·05) when compared with baseline breathing of room aim. Total haemoglobin and cytochrome aa₃ were unchanged from baseline. The accompanying arterial blood gas changes included significant increases in PaO₂ (30·9 mmHg, P<0·05), arterial O₂ saturation (11·7 per cent, P<0·05), and significantly decreased pH (−0·026, P<0·05), This study showed that NIRS can be used to continuously and non-invasively detect cerebral oxygenation changes in the live calf in response to both increased and decreased systemic arterial oxygen. Additionally, despite induction of profound hypoxaemia, cytochrome aa₃ in the brain did not appear to become reduced.     

Pharmacokinetics of fluconazole in cats after intravenous and oral administration

Fluconazole (100 mg) was administered to six adult cats as an intravenous infusion over 30 minutes, and the same cats received 100 mg of the drug orally 16 weeks later. The cats were bled repeatedly through an indwelling jugular catheter, the plasma fluconazole concentrations were assayed by high performance liquid chromatography, and the concentration-time data were subjected to a non-compartmental pharmacokinetic analysis. The mean (SD) intravenous half-life (13·8 [2·6] hours) was similar to that observed after oral dosing (12·4 [3·0] hours). The plasma clearances (intravenous 0·9 [0·1], oral 0·9 [0·2] ml min⁻¹ kg⁻¹) and the volumes of distribution at steady state (intravenous 1·1 [0·1], oral 1·0 [0·1] litre kg⁻¹) were also similar after the two routes of dosing. The peak plasma concentration was reached 2·6 hours after oral dosing and the drug was completely bioavailable (1·09 [0·05]). On the basis of this single dose study, the administration of 50 mg fluconazole every eight hours to a 4 kg cat should produce average steady state plasma fluconazole concentrations of approximately 33 mg litre⁻¹.     

Effects of inhibition of nitric oxide synthase on systemic arterial pressure and renal vascular resistance in cats

These studies were undertaken to examine the systemic and renal effects of the pharmacological inhibition of endothelium-derived nitric oxide (EDNO) in cats. In six healthy cats, the intravenous infusion of nitro-L-arginine at a dose of 100 μg kg⁻¹ bodyweight min⁻¹ resulted in a marked increase (P<0·001) in mean arterial pressure from the control value of 116·7 ± 4·6 mmHg to 154·2 ± 6·8 mmHg and an increase (P<0·05) in renal vascular resistance from the control value of 3·69 ± 0·33 mmHg min ml⁻¹ to 6·83 ± 1·15 mmHg min ml⁻¹. The increase in renal vascular resistance was generalised, with comparable increments in preglomerular and postglomerular vascular resistance. Mean values for glomerular capillary pressure (61·1 ± 61·9 vs 1·9 ± 1·6 mmHg), calculated from the sum of arterial colloid osmotic pressure plus proximal tubule stop-flow pressure, did not change in response to the infusion of nitro-L-arginine. However, there was a marked reduction in renal blood flow (29·4 ± 3·1 to 16·9 ± 2·3 ml min⁻¹, P<0·01) and glomerular filtration rate (5·22 ± 0·57 to 3·52 ± 0·45 ml min⁻¹, P<0·01). These results provide evidence that EDNO plays an important role in the basal regulation of systemic arterial blood pressure and renal haemodynamics in cats.     

α-Lipoic acid supplementation enhances heat shock protein production and decreases post exercise lactic acid concentrations in exercised standardbred trotters

Heat shock protein (HSP) expression is an adaptive mechanism against the disruption of cell homeostasis during exercise. Several antioxidant supplementation strategies have been used to enhance tissue protection. In this study, we examined the effects of a redox modulator, α-lipoic acid (LA) on HSP responses in six standardbred trotters following intense aerobic exercise. DL–LA supplementation (25 mg kg⁻¹ d⁻¹) for five weeks increased the resting levels of HSP90 (1.02 ± 0.155 in control and 1.26 ± 0.090 after supplementation in arbitrary units) and the recovery levels of inducible HSP70 (0.89 ± 0.056 in control and 1.05 ± 0.089 after supplementation in arbitrary units) in skeletal muscle. Furthermore, LA increased skeletal muscle citrate synthase activity at rest and lowered the blood lactate concentration during exercise without any changes in the heart rate. LA had no effect on concentrations of HSP60, HSP25 or GRP75 in skeletal muscle. LA decreased the exercise-induced increases in plasma aspartate aminotransferase and creatine kinase concentrations during recovery. Our results suggest that LA supplementation may enhance tissue protection and increase oxidative capacity of the muscle in horse.     

Effect of stage of gestation and foetal number on plasma concentrations of a pregnancy serum protein (PSP-60) in cattle

This study characterised the peripheral plasma concentration of PSP-60 throughout gestation, and examined the effect of stage of gestation and foetal number on this protein in Holstein cows after non-surgical embryo transfer. Cows (n=12) were divided into two groups; Group 1 contained single embryo recipient cows (n=5), Group 2 contained twin-embryo recipient cows (n=7). Blood was collected approximately every third day from day 0 (first day of standing oestrus), then daily for the last 10 days of gestation and until one day post-partum. Two of the twin-embryo recipient cows had abnormal pregnancies, consequently data from them was considered separately. In both groups PSP-60 increased progressively from about day 20 post-oestrus to 20 days pre-partum (from 0·9 ± 0·2 to 49·7 ± 8·7 ng ml⁻¹, and from 1·3 ± 0·6 to 115 ± 34·9 ng ml⁻¹ (mean ± SEM), in singleton and twin-bearing groups, respectively). The mean concentrations between 20 and 10 days pre-partum increased dramatically by about six-fold (P<0·001) in singleton-bearing cows (from 49·7 ± 8·7 ng ml⁻¹ to 2838 ± 73·7 ng ml⁻¹) to over two-fold in twin-bearing cows (from 115 ± 34·9 ng ml⁻¹ to 284 ± 98·2 ng ml^-1). The mean concentrations of the two groups were indistinguishable between 10 days pre-parturn and parturition. Cows giving birth prematurely to stillborn calves or to a schistosomus reflexus calf exhibited abnormal PSP-60 profiles. Our findings indicate that peripheral plasma PSP-60 concentrations are correlated to the stage of gestation and foetal number, and assist in predicting foeto-placental viability.     

Biochemical and haematological changes following prolonged halothane anaesthesia in horses

Six healthy horses were anaesthetised with halothane (1·2 times the horse minimal alveolar concentration) in oxygen for more than 12 hours. Serum bilirubin, aspartate aminotransferase, alkaline phosphatase and L-iditol dehydrogenase values were significantly (P<0·05) increased for up to nine days after anaesthesia. These changes suggest au anaesthesia related liver dysfunction. Creatine kinase increased to an average of more than 1400 iu litre⁻¹ 24 hours after anaesthesia and this change is indicative of muscle cell disruption. Renal-associated biochemical results, (that is serum creatinine and inorganic phosphate concentrations) were significantly increased transiently and are indicative of reduced renal function during and immediately after anaesthesia. Plasma concentrations of eicosanoids (6-keto-PGF_1a, PGF_2a, pge and thromboxane) following anaesthesia were not different from preanaesthetic values. The magnitude of liver and muscle cell related increases in serum enzyme activities resulting from prolonged halothane anaesthesia was in excess of that previously reported for anaesthesia of shorter duration.     

Effects of reducing dietary ([Na + K − [Cl + SO4]) on bone in dairy cows at parturition

The effects of feeding diets with different milliequivalents (mEq) of dietary ([Na⁺ + K⁺] − [Cl⁻ + SO₄⁼]) to dairy cows during the last seven weeks of pregnancy on bone morphology at parturition were studied. Nine monozygotic twin pairs of pregnant cows (five pairs of parity 1 or 2 and four pairs of parity 3 or more) were allocated to two diets which were formulated to provide either −4 mEq (anion diet) or +572·5 mEq (cation diet) of ([Na⁺ + K⁺] − [Cl⁻ + SO₄⁼]) kg⁻¹ dietary dry matter. Bone biopsies were taken from the tuber coxae between three and eight hours after parturition. The plasma concentrations of calcium and inorganic phosphorus, the total plasma alkaline phosphatase activity and the urinary hydroxyproline:creatinine ratio were not significantly affected by diet during the experimental period. In low parity (2 or less) cows the percentage trabecular bone volume, the percentage osteoclast surface and the mean number of osteoclasts per microscopic field (identified by Goldner staining) were lower on the anion diet than on the cation diet (P<0-02). In the high parity cows, the percentage osteoid volume (P<0·05) and the ratio of percentage osteoid volume to percentage osteoid surface (P<0·001) were greater in the cows fed the anion diet than in the cows fed the cation diet. The results show that reducing the mEq of dietary ([Na⁺ + K⁺] − [Cl⁻ + SO₄⁼]) to −4 mEq kg⁻¹ dietary dry matter affected some of the parameters of bone formation but did not enhance bone resorption.     

Carnosine, anserine and taurine contents in individual fibres from the middle gluteal muscle of the camel

High muscle camosine and anserine contents contribute significantly to intra-cellular physico-chemical buffering. Our aim was to measure carnosine, anserine and taurine contents directly in individual type I, HA and IIB fibres from the middle gluteus muscle of the camel. Mean carnosine contents in type I, IIA and IIB were 24·6 ±9·2, 39·4 ±11·4 and 42·8 ±18·8 mmol kg⁻¹ dry weight (dw), respectively. Mean anserine contents in type I, HA and IIB fibres were 30·0 ±8·4, 37·3 ±10·1 and 34·5 ±9·7 mmol kg⁻¹ dw, respectively. Mean taurine contents in type I, IIA and HB fibres were 42·4 ±15-9, 203 ±12·9 and 24·7 ±15·9 mmol kg⁻¹ dw, respectively. Higher carnosine contents in type II fibres emphasise the importance of carnosine to intra-muscular acid-base regulation. A specific role for taurine in type I fibres is unclear.     

Saliva collection and relationship between lactate concentration in blood and saliva of exercising horses

The aims of this study were to examine whether sufficient volumes of saliva can be collected for lactate analysis in exercising horses and to establish the relationship between blood and saliva lactate concentration. Saliva was collected for 30 seconds after exercise with a commercially available cotton swab Salivette^® from two sites: At the height of the 3rd premolar in the maxilla (upper site) and under the tongue at the level of the frenulum linguae (lower site). Blood was taken from the jugular vein. Horses were submitted to two types of standardized exercise on a treadmill: Continuous exercise and an incremental exercise step test.The average (± standard deviation) volume of saliva sampled at the upper site was 390±280 μl, and at the lower site 450±310 μl. The lactate concentration in saliva after continuous exercise sampled at the upper site was 2.21±1.97 mmol/l, and at the lower site it was 3.76±2.31 mmol/l. There was no significant correlation between lactate concentration in blood and upper site saliva, and blood and lower site saliva after continuous exercise and during incremental exercise step test.     

Disposition and urinary excretion of phenylbutazone in normal and febrile greyhounds

Five days after the induction of acute systemic inflammation in greyhounds by intramuscular and subcutaneous injections of Freund's adjuvant, the hepatic concentrations of cytochromes P-450 and b₅, the activities of the hepatic microsomal enzymes aniline p-hydroxylase and aminopyrine n-demethylase and the disposition and urinary excretion of phenylbutazone were determined. The mean plasma concentrations of phenylbutazone after intravenous administration were described by the bi-exponential equations: Cp = 144·2e^−34·6t + 171·5e^−0·104t for five normal greyhounds and Cp = 113·6e^−16·13t + 163·1e^−0·108t for five febrile greyhounds. The elimination half-lives, total body clearances and apparent volumes of distribution were 6·7 hours, 18·4 ml kg⁻¹ hour⁻¹ and 0·18 litre kg⁻¹, for the normal greyhounds, and 6·4 hours, 19·5 ml kg⁻¹ hour⁻¹ and 0·18 litre kg⁻¹, for the febrile greyhounds. There were no significant differences between the pharmacokinetic parameters describing the distribution and elimination of phenylbutazone, or between the quantities of phenylbutazone, oxyphenbutazone and hydroxyphenylbutazone excreted in the urine. In the febrile greyhounds, there were significant decreases in the hepatic microsomal concentrations of cytochromes P-450 and b₅ and in the activities of aniline p-hydroxylase and aminopyrine n-demethylase.     

Epidermal and hepatic glucocorticoid receptors in cats and dogs

Low capacity, high affinity [³H] dexamethasone binding receptors were identified in cytosolic preparations of liver (mean number 45±10·1 fmol mg⁻¹ protein, apparent dissociation constant 0·4±0·1 nM) and skin (mean number 46·4±23·8 fmol mg⁻¹ protein, apparent dissociation constant 1 ± 0·2 nM) of clinically normal dogs. For clinically normal cats, approximately half these numbers of receptors with a lower affinity, were detected in liver (mean number 23·1±10·4 fmol mg⁻¹ protein, apparent dissociation constant 3·2±0·9 nM) and skin (mean number 23·90±10·9 fmol mg⁻¹ protein, apparent dissociation constant 2·2±1·5 nM). This difference between dogs and cats in [³H] dexamethasone binding receptors may contribute to the relative glucocorticoid resistance observed in cats.     

Effect of acute induced metabolic alkalosis on the acid/base responses to sprint exercise of six racing greyhounds

To investigate the effect of acute induced metabolic alkalosis on the haematological, biochemical and metabolic responses to sprint exercise, six greyhound dogs with previously placed carotid artetrial catheters were raced four times over a distance of 400 metres. Each dog was raced twice after receiving oral sodium bicarbonate solution (NaHCO₃) (400 mg kg⁻¹) or lactated Ringer's solution ( ). Before, and for intervals of up to one hour after, the exercise arterial blood samples were collected for the measurement of blood gases, packed cell volume, total protein, serum biochemistry and plasma lactate. The time to complete the 400 metre sprint ranged from 32·7 seconds to 36·9 seconds. There was no significant difference in racing times between the dogs treated with NaHCO₃ and , and there was no significant difference between the plasma lactate measurements after the treatments with NaHCO₃ or . Serum chloride concentrations were significantly lower after NaHCO₃ than after LRS, and there was a trend towards a lower serum potassium concentration after NaHCO₃ treatment. Plasma lactate concentrations showed a similar increase and time course of disappearance after both LRS and NaHCO₃ treatments. There were significant changes in all the parameters measured after the exercise, but there were large variations between individual dogs and between races when the dogs were receiving the same treatment.     

Penetration of amoxycillin to the respiratory tract tissues and secretions in Actinobacillus pleuropneumoniae infected pigs

The pharmokinetic properties of amoxycillin, and its penetration into respiratory tract tissue, were determined in 18 Actinobacillus pleuropneumoniae infected pigs, after a single i.v. dose of 8·6 mg amoxycillin kg⁻¹ bodyweight. Pleuropneumoniae was produced experimentally in pigs by an aerosol infection model. The infection created a homogenous response, characterised by depression of breathing and increased body temperature. The clinical symptoms were accompanied by increased haptoglobin levels and circulating white blood cell counts. At necropsy the findings were characterised by a bilateral fibrinous pleuropneumonia. Twenty hours after infection, the pigs were administered amoxycillin i.v. The plasma concentration-time curve was described by a three compartment open model. The mean residence time and the elimination half-life were l·5 and 3·4 hours, respectively. The steady-state volume of distribution was 0·67 litres kgl, and the clearance was 0·46 litres kg⁻¹ hour⁻¹. There were no significant differences between these values and those reported previously for healthy pigs. The concentration of amoxycillin in bronchial secretions, lung tissue and diseased lung tissue peaked two hours after intravenous drug administration, while amoxycillin concentration in pleural fluid, lymph nodes and tonsil tissue peaked at the first sampling point one hour after drug administration. The concentration of amoxycillin in secretions and tissue decreased by a slower rate than amoxycillin concentration in plasma, resulting in an increasing tissue-to-plasma concentration ratio. The distribution ratios (AUC_tissue/JAUC_plasma) was 0·53 for bronchial secretions, 0·44 for pneumonic lung tissue, 0·42 for lung tissue, 1·04 for pleural fluid, 0·58 for lymph nodes and 0·37 for tonsil tissue. The distribution of amoxycillin to secretions was increased compared with that previously reported for healthy pigs, while only minor changes were observed in lung tissue.     

The cardiopulmonary effects of clenbuterol when administered to dorsally recumbent halothane-anaesthetised ponies - failure to increase arterial oxygenation

Clenbuterol (0·8 μg kg ⁻¹ intravenously) was investigated in ponies (small horses) anaesthetised with acepromazine, detomidine and thiopentone, then halothane in oxygen alone (hyperoxic group) or with nitrous oxide (hypoxic group). Following instrumentation, ponies were placed in dorsal recumbency for 60 minutes, clenbuterol (both groups) or a saline control (hyperoxic group) given, and cardiopulmonary parameters monitored for a further 60 minutes. In the hyperoxic group, clenbuterol administration resulted in a transitory (<five minutes) 15 per cent fall in arterial blood pressure and 78 per cent rise in intramuscular blood flow. Heart rate increased from a mean of 42 (SD 4) to 54 (12) beats per minute, the rise being significant for 15 minutes. Cardiac index increased from 2·1 (0·7) to 3·-9 (0·7) litres m⁻² and remained significantly elevated for the remainder of the measurement period. Cardiovascular changes in the hypoxic group were similar. 30 minutes after clenbuterol administration, PaO₂ had changed non-significantly from 32·.3 (19·2) to 33·.4 (17) kPa in the hyperoxic group and from 7·9 (1·8) to 8·.6 (1·3) kPa in the hypoxic group. The study concludes that under these experimental conditions, clenbuterol does not cause significant improvement in arterial oxygenation, but its cardiovascular effects are minimal or advantageous.     

Pathology and residues in veal calves treated experimentally with clenbuterol

Six veal calves were medicated with clenbuterol at 20 μg kg bodyweight⁻¹ day⁻¹ for 42 days before they were slaughtered, to evaluate the lesions and residues in target organs. Compared with six unmedicated calves the most noticeable changes were tracheal dilatation, decreased uterine weight, slight mucous hypersecretion in the uterus and vagina and depletion of liver glycogen. The highest concentrations of clenbuterol (62 to 128 ng/g⁻¹) were recorded in the choroid/retina, and the aqueous humour had the lowest concentration (0·5 to 2·4 ng ml⁻¹). The residue concentrations were higher than the maximum residue level set for clenbuterol (0·5 ng g⁻¹)     

Endotoxin-induced platelet aggregation in heparinised equine whole blood in vitro

Endotoxaemia is a leading cause of death among horses. Thrombocytopenia is a common finding in clinical and experimentally-induced cases of endotoxaemia and can lead to coagulopathies, including disseminated intravascular coagulopathy which is usually fatal. In this study it was shown that endotoxin (3 ng ml⁻¹ to 25 μg ml⁻¹) can aggregate equine platelets in heparinised whole blood in vitro. The endotoxin-induced aggregation (EIA) was shown to be dependent on the presence of leucocytes in the blood and did not occur when detoxified endotoxin was used, suggesting that lipid A was necessary for the response. Aspirin (1 mmol litre⁻¹) had no effect on EIA whereas apyrase (40 μg ml⁻¹) completely abolished it and CV3988 (3 to 30 μmol litre⁻¹) (a competitive antagonist of platelet-activating factor) inhibited the response in a concentration-dependent manner. It is concluded that endotoxin activates equine platelets at low concentrations through an indirect mechanism that involves calcium, leucocytes, adenine nucleotides and platelet-activating factor.     

Lincomycin and spectinomycin in the treatment of breeding rams with semen contaminated with ureaplasmas

Ureaplasma species were isolated from semen samples collected sequentially from one Awassi and three Assaf breeding rams. Each ram was injected subcutaneously with an aqueous solution of lincomycin and spectinomycin for five consecutive days at a dose equivalent to 4·5 mg kg⁻¹ lincomycin and 9·0 mg kg⁻¹ spectinomycin daily. Serum and semen samples were collected at intervals during the treatment and assayed for lincomycin. No Ureaplasma species were isolated from semen samples collected during the course of the treatment and at intervals for 17 days after the last treatment. The concentration of lincomycin in semen ranged from 0·51 μg ml⁻¹ four hours after treatment to 0·08 μg ml⁻¹ 24 hours after treatment, and these levels were three to nine times higher than the corresponding serum concentrations.