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6个猪种胰岛素样生长因子-1(IGF-1)基因座位遗传多态性检测 总被引:16,自引:0,他引:16
本文采用PCR-RFLP分析方法对我国地方猪种民猪、香猪、桃源猪、内江猪、姜曲海猪以及用作对照的国外猪种杜洛克猪的IGF-1基因座位进行研究。结果发现:IGF-1基因PCR扩增片段存在2个限制性内切酶HhaI酶切位点,其中1个酶切位点产生遗传多态。此片段上共产生2个等位基因。在IGF-1基因位点上,香猪除与桃源猪基因频率差异不显著之外,与其它猪种之间基因频率差异极显著(P<0.01),而其它各猪种之间差异均不显著 相似文献
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猪恶性高温综合征(MHS)基因群体检测及部分片段DNA序列研究 总被引:4,自引:0,他引:4
本研究主要包括:(1)建立了猪MHS基因检测的PCR-RFLP方法。该方法具有准确、快速和无侵害的特点;(2)利用猪MHS基因的PCR-RFLP方法对中国现有猪种20个群体的MHS基因座位的基因和基因型频率进行了检测。结果表明,属于中国的本地品种五指山猪、香猪,引进品种约克夏猪,均不含有MHS基因;在中国本地品种二花脸猪和民猪中,发现存在MHS基因,且民猪的频率较高(0.1562);培育品种北京花猪,引进品种丹系长白猪和杜洛克猪,均有MHS基因;引进品种皮特兰猪、比系长白猪,MHS基因频率最高,分别为0.9520和0.9432;(3)猪RYR1/CRC基因部分DNA序列品种间比较,MHSNN香猪和二花脸猪的1843位为“C”,而MHSnn为“T”;同时还发现一些单碱基的差异 相似文献
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应用RT—PCR技术扩增禽传染性支气管炎病毒S1基因的研究 总被引:2,自引:0,他引:2
选择S1基因做为目的基因,运用RT-PCR技术分别扩增12株以非免疫鸡胚繁殖并通过超速离心浓缩的鸡胚尿囊液中的IBV,所用引物为IBV Beaudette株S1基因两侧的对应序列,跨幅为1.72kb。结果6株IBV毒株(SAIB3、F、D41、H52、Holte)扩增出了长约1.7kb的S1基因片段,而另6株IBV毒株虽经4次RT-PCR重复后也显阴性。用HaeⅢ内切酶对6个毒株的RT-PCR产物 相似文献
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在国内首先将氟烷基因型PCR-RFLP诊断技术用于种猪性能测定中心的种猪测定。通过从猪毛、猪血中提取的DNA,用特异性的引物进行PCR扩增均能得到659bp的DNA片断,并用HhaⅠ内切酶消化PCR产物,酶切结果能准确检测猪应激综合症,即RYR1基因(氟烷基因)的突变。在测定的长白、约克、杜洛克猪中,发现长白猪Ha1^n基因基因频率较其它品种高,为9.0%,杜洛克为4.2%,在大约克中未发现。 相似文献
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我国鸡传染性支气管炎病毒地方性流行毒株S1基因的RT-PCR扩增及其克隆与鉴定 总被引:2,自引:0,他引:2
采用RT-PCR方法,以IBVS1全基因特异性引物分别从我国华东(HD)、华北(HB)、华中(HZ)、华南(HN)、西北(XB)及东北(DB)等地的IBV流行株基因组中扩增出预期的1.7kb左右的DNA片段。PCR产物的HaeII酶切分析及其与英国IBVS1全基因核酸探针的分子杂交证实所获6个IBV流行株的PCR产物为IBVS1基因。将此6个毒株的S1基因PCR产物分别进行5’和3’端的BamHI和HindII酶切识别位点的分子修饰之后插入到克隆质粒pUC18的BamHI/HindII位点,在大肠杆菌中实现了目的基因的分子克隆。S1基因的RFLP分析表明我国IBV已有分子水平的变异。 相似文献
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我国鸡传染性支气管炎病毒地方性流行毒株S1基因的RT—PCR扩增及其 … 总被引:1,自引:0,他引:1
采用RT-PCR方法,以IBV S1全基因特异性引物分别从我国华东,华北,华中,华南,西北及东北等地的IBV流行株基因组中扩增出预期的1.7kb左右的DNA片段。PCR产物的HaeⅢ酶切分析及其与英国IBV S1全基因核酸探针的分子杂交证实所获6个IBV流行株的PCR产物为IBV S1基因。将此6个毒株的S1基因PCR产物分别进行5‘和3’端的BamHI和HindⅢ酶切识别位点的分子修饰之后插入到 相似文献
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Kim TH Kim KS Choi BH Yoon DH Jang GW Lee KT Chung HY Lee HY Park HS Lee JW 《Journal of animal science》2005,83(10):2255-2263
To understand molecular genetic characteristics of Korean pigs, the genetic relationships of nine pig breeds including two Korean pigs (Korean native pig and Korean wild pig), three Chinese pigs (Min pig, Xiang pig, and Wuzhishan pig), and four European breeds (Berkshire, Duroc, Landrace, and Yorkshire) were characterized from a 16-microsatellite loci analysis. The mean heterozygosity within breeds ranged from 0.494 to 0.703. Across multiple loci, significant deviation from Hardy-Weinberg equilibrium was observed in most pig breeds, except for two Chinese pigs (Min pig and Wuzhishan pig). This deviation was in the direction of heterozygote deficit. Across population loci, 36 of 144 significantly deviated (P < 0.05) from Hardy-Weinberg equilibrium. The mean FST, a measure of genetic divergence among subpopulations, of all loci indicated that 26.1% of total variation could be attributed to the breed difference. Relationship trees based on the Nei's DA genetic distance and scatter diagram from principal component analysis consistently displayed pronounced genetic differentiation among the Korean wild pig, Xiang pig, and Wuzhishan pig. Individual assignment test using a Bayesian method showed 100% success in assigning Korean and Chinese individual pigs into their correct breeds of origin and 100% exclusion success from all alternative reference populations at P < 0.001. These findings indicate that the Korean native pig has been experiencing progressive interbreeding with Western pig breeds after originating from a North China pig breed with a black coat color. Considering the close genetic relationship of Korean pigs to the Western breeds such as Berkshire and Landrace, our findings can be used as valuable genetic information for the preservation and further genetic improvement of the Korean native pig. 相似文献
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基于香猪全基因组重测序数据,在白细胞表面抗原CD53基因中筛选到一个结构变异(structure variant,SV)184,为了探索不同猪品种间此结构变异是否存在多态性变化,本试验选择香猪、大白猪、糯谷猪、柯乐猪、江口萝卜猪和荣昌猪作为试验动物,采用PCR方法对猪群中SV184的分布频率进行比较研究,应用RT-PCR技术,分析CD53基因的原初转录本序列结构,研究SV184对CD53基因的转录是否有直接影响。结果显示,6个猪品种中SV184的分布频率存在明显差异,香猪以VN基因型为主,其他5个猪品种是NN基因型占优势;计算两种等位基因的频率,香猪以缺失的V等位基因为主,其他5个猪品种以正常的N等位基因为主。选择3种基因型个体的血液样本,逆转录后分析CD53基因的原初转录本的序列,从中检测到缺失型和不缺失的两种转录本形式,提示SV184对CD53基因的转录无直接影响。SV184可作为分子标记用于区分香猪和其他猪品种。 相似文献
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为了探讨BOLL-I6-sv285(15号染色体BOLL基因第6内含子)和STRA8-I4-sv447(18号染色体STRA8基因第4内含子)这两个结构变异在地方猪与欧洲猪品种中的群体分布差异,试验采用PCR方法对从江香猪、江口萝卜猪、荣昌猪和大白猪4个猪品种进行基因分型,并通过在线软件miRBase、UCSC、RegRNA 2.0对结构变异序列所含的功能元件进行分析。结果显示,BOLL-I6-sv285为一段285 bp的插入,经群体验证,3个地方猪品种以DI和DD基因型为主,I等位基因的频率高于大白猪,插入基因型(Ⅱ)对应较高的产仔数,基因型与从江香猪产仔数之间呈显著相关(P<0.05);STRA8-I4-sv447为一段447 bp的插入突变,群体中DD基因型占优势,从江香猪、江口萝卜猪和大白猪的I等位基因频率高于荣昌猪,插入基因型(Ⅱ)对应较低的产仔数,基因型与从江香猪产仔数之间呈显著相关(P<0.05)。本试验鉴定的BOLL-I6-sv285和STRA8-I4-sv447两个结构变异可作为从江香猪产仔数的候选分子标记。 相似文献
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China is one of the most diverse countries, which have developed 88 indigenous pig breeds. Several studies showed that pigs were independently domesticated in multiple regions of the world. The purpose of this study was to investigate the origin and evolution of Chinese pigs using complete mitochondrial genomic sequences (mtDNA) from Asian and European domestic pigs and wild boars. Thirty primer pairs were designed to determine the mtDNA sequences of Xiang pig, Large White, Lantang, Jinhua and Pietrain. The phylogenetic status of Chinese native pigs was investigated by comparing the mtDNA sequences of complete coding regions and D-loop regions respectively amongst Asian breeds, European breeds and wild boars. The analyzed results by two cluster methods contributed to the same conclusion that all pigs were classified into two major groups, European clade and Asian clade. It revealed that Chinese pigs were only recently diverged from each other and distinctly different from European pigs. Berkshire was clustered with Asian pigs and Chinese pigs were involved in the development of Berkshire breeding. The Malaysian wild boar had distant genetic relationship with European and Asian pigs. Jinhua and Lanyu pigs had more nucleotide diversity with Chinese pigs although they all belonged to the Asian major clade. Chinese domestic pigs were clustered with wild boars in Yangtze River region and South China. 相似文献
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荣昌猪是我国优良地方品种,巴马香猪和贵州小型香猪为我国独特小型猪品系。本研究采用美国猪基因组协作计划推荐的19个微卫星标记对荣昌猪、荣昌猪B系、巴马香猪、贵州小型香猪及外来猪种大白猪进行了遗传学检测和分析。结果表明:19个微卫星位点在群体中均表现为多态,每位点等位基因数10~23个。5个猪种中荣昌猪B系的遗传多样性最高,荣昌猪次之;2种小型猪的遗传多样性较低,其中巴马香猪的等位基因数(121个)略大于贵州小型香猪(114个),但其遗传多样性指数(平均期望杂合度0.6535±0.0347)小于贵州小型香猪(0.6919±0.0227),反映了巴马香猪较低的基因杂合度和较小的遗传变异;大白猪的遗传多样性最低。从品系的共有等位基因来看,荣昌猪B系基因背景组成中其亲本品系荣昌猪所占比例(30.22%)要大于大白猪(24.37%);大白猪和其他4个猪群体的共享等位基因数均较低(21.24%~25.12%);巴马香猪和贵州小型香猪之间共有等位基因数最高(45.06%)。采用基于遗传距离的NJ法和基于基因频率的最大似然法进行系统聚类,除了大白猪明显为独立分支及荣昌猪B系表现出较远的聚类关系外,其他3个猪种间的聚类有所不同。 相似文献
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WANG Yang MEI Zi-ling YU Chang-yan NIU Xi XIE Ling-ling NIU Qiu-jin QIU Zhi-lang WANG Jia-fu RAN Xue-qin 《中国畜牧兽医》2016,43(5):1294-1301
To explore the functional genes affecting the growth and development of Xiang pig, a single nucleotide polymorphism (SNP) site rs80894395, located at intron 10 of polypeptide N-acetylgalactosaminyltransferase 2 (GALNT2) gene was screened out by Illumina Porcine SNP60K chip, which showed a weak association with the abdomen circumference of pig.Further, the genotypes of site rs80894395 in 520 female Xiang pigs were detected using allele-specific PCR(AS-PCR) method, taking 43 Yorkshire pigs and 36 Kele pigs as control groups.All of CC, CT and TT genotypes were detected from the three pig breeds.The dominant genotype of Xiang pig was CT genotype and it was positively weak-associated with its body measurement indexes including body weight, body length, and abdomen circumference.The CC genotype was dominant genotype in Yorkshire pig, and the frequency of T allele was extremely significant decreased compared with that of Xiang pig (P<0.01).There was no difference in the frequencies of CC and CT genotypes in Kele pig, and its allele frequencies was not significantly different compared with that of Xiang or Yorkshire pig breeds (P>0.05).A splice-site nearby site rs80894395 was found out using bioinformatics prediction software, which might affect the splicing process of GALNT2 gene.It suggested that site rs80894395 might have a connection with the growth and development of Xiang pig. 相似文献
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为了探究影响香猪生长发育的功能基因,本试验利用Illumina Porcine SNP60K芯片筛选出N-乙酰氨基半乳糖转移酶2(polypeptide N-acetylgalactosaminyltransferase 2,GALNT2)基因内含子10中一个与腹围呈弱相关的单核苷酸多态性(single nucleotide polymorphism,SNP)位点rs80894395。在此基础上应用等位基因特异性PCR(allele-specific PCR,AS-PCR)技术检测520头雌性香猪群体中该位点的基因型,并与43头大白猪、36头柯乐猪相比较。结果表明,从3个猪群体中检测到rs80894395位点的CC、CT和TT3种基因型;其中香猪群体以CT为优势基因型,且基因型与香猪的体重、体长和腹围呈弱的正相关;大白猪群体以CC基因型为主,T等位基因频率较香猪极显著减少(P<0.01);柯乐猪群体中CC与CT基因型频率相等,与香猪和大白猪的基因频率间的差异不显著(P>0.05)。生物信息学分析表明,rs80894395位点可能影响GALNT2基因的剪接过程。rs80894395与香猪的生长发育可能有一定的联系。 相似文献
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为了研究KIT基因的拷贝数与贵州地方猪毛色之间的关系,本试验以黔北黑猪、柯乐猪、糯谷猪、香猪4个地方品种为试验材料,采用实时荧光定量PCR方法检测基因组中KIT基因的拷贝数变异,并与荣昌猪和大白猪2个白毛色猪品种相比较.结果显示,4个贵州地方猪种中,全黑/全棕毛色个体的KIT基因拷贝数以缺失为主,黑色带白斑个体的KIT基因拷贝数全部缺失,而棕色带白个体的KIT基因拷贝数增加、正常各半.大白猪的KIT基因拷贝数显著增加,荣昌猪KIT基因拷贝数以正常类型为主.表明大白猪的白毛色由KIT基因拷贝数增加决定,但荣昌猪的白毛色可能与KIT基因的拷贝数变异之间没有直接的联系;4个贵州地方品猪的全黑/全棕色毛色与KIT基因拷贝数的缺失有关,但柯乐猪和香猪中带白斑个体的KIT基因拷贝数并未增加,提示贵州地方猪品种的白斑毛色的形成机制有一定的特殊性. 相似文献