猪副嗜血杆菌病的诊治

猪副嗜血杆菌病是由猪副嗜血杆菌引起的传染性疾病,对断奶前后仔猪的影响较大,如果猪感染上猪副嗜血杆菌,就会出现咳嗽、呼吸困难、急剧消瘦等情况。随着我国动物养殖行业的发展,养猪业逐渐向规模化、集约化的方向发展,猪副嗜血杆菌病的发病率呈现出上升的趋势,对养猪业造成了严重的影响。对一起猪副嗜血杆菌病的诊治情况进行简单介绍,希望能够为广大养殖者提供参考。     

猪副嗜血杆菌病的治疗过程

猪副嗜血杆菌病是猪养殖过程中经常出现的一种传染性疾病。该种疾病发生受到很多因素影响,其中饲养管理因素和致病菌因素是导致该种疾病发生的主要因素。近些年,随着猪养殖产业不断向前发展,猪副嗜血杆菌病的发病率不断增加,已经成为严重威胁猪养殖产业健康发展的主要疾病之一。笔者主要结合实际案例,就猪副嗜血杆菌病的诊断和治疗进行分析。     

细菌生物膜对副猪嗜血杆菌致病机制的影响

近年来,随着养殖模式的转变和免疫抑制病的出现,由副猪嗜血杆菌引起的副猪嗜血杆菌病成为养猪场常见的、多发的并且危害严重的猪呼吸系统疾病之一。因临床抗生素的滥用,导致细菌的耐药性越来越强,且由细菌引起的疾病越来越难以控制,以至治疗成本也越来越高。副猪嗜血杆菌在猪体内具有一定的耐药性和慢性感染的能力,其中,细菌生物膜的存在增强了该菌的致病性和耐药性[1]。     

猪瘟继发副猪嗜血杆菌病的诊断与防治

猪瘟继发副猪嗜血杆菌病是养殖业中的常见疾病,尤其是猪瘟和副猪嗜血杆菌病都是免疫抑制类的疾病,具有诊断难度大的特点.发病之后可能在养殖场内迅速的扩散和蔓延,死亡率比较高,会给养殖户带来很大的经济损失.为此,应该加大对猪瘟以及副猪嗜血杆菌病的诊断和方法力度,减少猪瘟继发副猪嗜血杆菌病的不利影响,推动猪养殖业的健康发展.     

广西副猪嗜血杆菌病病原菌的分离鉴定与药敏试验

副猪嗜血杆菌病又称纤维素性浆膜炎和关节炎,其病原为副猪嗜血杆菌(H.parasuis,HPS),是一种革兰氏阴性短小杆菌,属于巴斯德菌科嗜血杆菌属[1].随着养猪业的发展,规模化养殖和饲养的高度密集化,以及呼吸道综合征等因素,使得该病流行日趋严重.近年来,我国由副猪嗜血杆菌引起猪多发性浆膜炎和关节炎的报道屡见不鲜.笔者对广西规模化猪场的疑似副猪嗜血杆菌感染的病料进行了分离鉴定及药敏试验.现将结果报告如下.     

副猪嗜血杆菌病的防治

副猪嗜血杆菌病也被称为格拉泽氏病或多发性纤维素性关节炎和脑膜炎,该病主要以关节炎、关节肿胀、呼吸道症状为临床表现,是生猪比较高发的一种细菌性疾病。基于此,下文笔者首先分析了副猪嗜血杆菌病的病原、临床症状、病理变化、疾病诊断,然后着重探讨了副猪嗜血杆菌病的防治措施,以期为广大生猪养殖人员提供参考。     

规模化猪场如何防控副猪嗜血杆菌病

正目前副猪嗜血杆菌病发病率高,对规模化猪场影响很大,尤其是对保育猪的影响更大,使猪场的生产水平下降,经济效益降低,那么如何防控好副猪嗜血杆菌病,下面笔者做如下论述。1副猪嗜血杆菌病的概述副猪嗜血杆菌病又称革拉氏病,是由副猪嗜血杆菌引起的猪的一种以多发性浆膜炎、脑膜炎和关节炎为主要症状的细菌传染病。目前副猪嗜血杆菌病在我国广泛流行,是导致保育猪发病、死亡的主要原因。2副猪嗜血杆菌病的临床流行特点     

副猪嗜血杆菌的临床分离鉴定与耐药性分析

副猪嗜血杆菌是副猪嗜血杆菌病的病原菌,主要引起猪发生以纤维素性浆膜炎、多发性关节炎和脑膜炎为特征的传染病.随着世界养猪业的发展,副猪嗜血杆菌病已成为全球范围内影响养猪业的典型细菌性疾病之一.     

猪副嗜血杆菌病及混合感染的防控

<正>目前,我国猪副嗜血杆菌病流行日趋严重,是影响养猪业的主要细菌病之一。猪副嗜血杆菌病又称多发性纤维素性浆膜炎和关节炎,也称格拉泽氏病。是由猪副嗜血杆菌引起,肺原支原体、猪流感病毒、伪狂犬病毒和圆环病毒等都可以促进或加重猪副嗜血杆菌病的感染。笔者就曾见几种副嗜血杆菌和其他病混合     

规模化猪场副猪嗜血杆菌病的诊断及综合防制

副猪嗜血杆菌病是由副猪嗜血杆菌引起的猪的一种以多发性浆膜炎、关节炎及高死亡率为特征,严重危害仔猪和生长猪的传染性疾病。随着世界养猪业的发展,该病已成为全球范围内影响养猪业的典型细菌性疾病,给养猪业造成的损失相当惨重。笔者就规模化猪场副猪嗜血杆菌病的诊断、治疗和综合防制等方面作一简要综述。     

Serovar profiling of Haemophilus parasuis on Australian farms by sampling live pigs

Objective Investigate the diversity of serovars of Haemophilus parasuis (Hps) present in Australian pig herds. Design Nasal swabs were used to obtain multiple isolates of Hps, which were grouped first by genotyping and then by serotyping representative isolates. Procedure Swabs were taken from the nasal cavity of just-weaned healthy pigs from multiparous sows on 12 farms and from post-weaned pigs of multiparous sows on 1 farm. On 5 of the 13 farms, nasal swabs were also obtained from pigs showing clinical signs suggestive of Glässer's disease. On a further 7 farms, nasal swabs were obtained only from pigs with clinical signs suggestive of Glässer's disease. Results A total of 556 Hps isolates were genotyped, and 150 isolates were serotyped. Hps was detected on 19 of the 20 farms, including 2 farms with a long history of freedom from Glässer's disease. Isolates of Hps belonging to serovars regarded as potentially pathogenic were obtained from healthy pigs at weaning on 8 of the 10 farms with a history of Glässer's disease outbreaks. Sampling 213 sick pigs yielded 115 isolates of Hps, 99 of which belonged to serovars that were either potentially pathogenic or of unknown pathogenicity. Only 16 isolates from these 213 sick pigs were of a serovar known to be non-pathogenic. Conclusion Healthy pigs contain a range of Hps serovars, even on farms free of Glässer's disease. Nasal swabbing of both healthy and sick pigs seems a useful method of serovar profiling of farms.     

Serological characterisation of Haemophilus parasuis isolates from Australian pigs

A total of 31 isolates of Haemophilus parasuis obtained from Australian pigs were serotyped by the Kielstein-Rapp-Gabrielson scheme. The isolates were assigned to serovar 1 (1 isolate), serovar 2 (1 isolate), serovar 4 (4 isolates), serovar 5 (7 isolates), serovar 9 (2 isolates), serovar 10/7 (4 isolates), serovar 12 (1 isolate) and serovar 13 (6 isolates). The remaining 5 isolates could not be assigned to a serovar. Two different serovars (5 and 13) were detected in one herd. The only 2 isolates obtained from clinically normal pigs (from the same herd) were serovar 9. The common serovars were isolated from pigs with pneumonia as well as from pigs with conditions of the Glässer's disease type. The serological heterogeneity amongst Australian isolates of H parasuis has important implications for the use of vaccines to control Glässer's disease.     

Update on the pathogenesis of Haemophilus parasuis infection and virulence factors

Haemophilus parasuis is the causative agent of Glässer's disease in pigs, a severe systemic disease that has led to increasing economic losses in the pig industry worldwide. The H. parasuis genome sequence has been completed, but the function and essentiality of the annotated genes remain largely unknown, especially virulence factors. The recent developments in the efficient genetic manipulation of H. parasuis have greatly facilitated the study of gene function, pathogenesis mechanisms and virulence factors. In this review, we provided update information regarding that (i) how the pathogen overcome host immune responses and cell barriers which were tightly associated with the pathogenesis, and (ii) the several recent identification of virulence factors were involved in evading the immune responses and cell barriers in H. parasuis.     

Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

Haemophilus parasuis causes contagious porcine Glässer's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H. parasuis.     

Immunoproteomic analysis of the protective response obtained with subunit and commercial vaccines against Glässer's disease in pigs

An immunoproteomic analysis of the protective response of subunit and commercial vaccines in colostrum-deprived pigs against Glässer's disease was carried out. A mixture of proteins with affinity to porcine transferrin (PAPT) from Haemophilus parasuis Nagasaki strain (serovar 5) was inoculated intramuscularly (PAPT_M) and intratracheally (PAPT_Cp), along with a commercial bacterin. PAPT were separated using 2 dimensional electrophoresis (2DE) gels and with them, 2DE Western blots were carried out. A total of 17 spots were identified as positive with sera of pigs from any of the three vaccinated groups, the highest number of immunoreactive proteins being detected in those having received PAPT_Cp. Among them, six proteins (FKBP-type peptidyl-prolyl cis-trans isomerase, neuraminidase exo-α-sialidase, xanthine-guanine phosphoribosyl transferase, CMP-N-acetylneuraminic acid synthetase, phenylalanyl-tRNA synthetase and glyceraldehyde 3-phosphate dehydrogenase) were found to be novel immunogens in H. parasuis. These proteins showed a high potential as candidates in future subunit vaccines against Glässer's disease. The three experimental groups developed specific systemic total IgG (IgGt), IgG1, IgG2 and IgM antibodies after immunizations. In addition, those receiving PAPT_Cp yielded a serum IgA response.     

养猪场的生物安全方案浅析

随着我国社会经济的快速发展,我国畜牧业向着规模化、集约化不断发展,出现了越来越多的养殖集团和养殖集中小区。但疫病流行越来越引人注目,禽流感、布鲁氏菌感染时常成为社会焦点,特别是2018年开始的非洲猪瘟疫情肆虐,至今严重困扰养猪业和广大市民的菜篮子安全。如何有效控制和防御动物疫病的发生及传播,减少疫病的传播和流行,降低疾病对养殖业的损害和压力,清除净化动物重大流行疫病,建立全面的养猪场的生物安全体系,已成为行业从业者共同关注的课题。文章重点对如何建立生物安全体系进行详细阐述。     

牛瘤胃积食的综合诊断与防治措施

随着人民物质生活的不断提高,畜牧业的持续发展,人们对于畜禽,尤其是大动物的养殖变得习以为常,这也促使着对于牛的养殖更多的是由传统的放牧、散养,转变为集约化、家庭农场式的现代化养殖,现代化养殖又多选用舍饲、栓养,其养殖密度、活动量又得不到较好的保证,这便导致在牛的养殖中,极易出现机体消化系统功能障碍的问题,尤其是牛瘤胃积食,其易复发、难根治的特性,十分影响畜牧业的健康发展,因此畜牧工作者需了解关于牛瘤胃积食的发生原因、临床特征,掌握其诊断方法和综合的防治措施,来降低牛瘤胃积食的发病率和病死率,进而达到促进畜牧业健康发展的目的。     

产业转型期规模猪场建设工程防控技术思考

非洲猪瘟和环保的双重影响刺激了中国养猪业快速向集约化、高效化和自动化方向转型,而舍间交叉感染、疫病难控、环境污染等问题依然未能得到有效解决。在猪场建设和管理中,人们的关注重点主要有疾病控制、粪污臭气治理和无抗饲料三个方面,分别对应着生物安全、环保安全和食品安全三个安全底线。在重视猪场生物安全体系建设时,人们往往忽视工程防控技术措施的整体应用,其贡献尚无法得以很好的体现。文章从工程技术的角度,分析了隔离、合理分区和防止交叉感染等对猪场生物安全防控的作用,为猪场的工程防疫体系建设、守住三个安全底线提供指导。     

益母草汤加减治疗20例白牦牛胎衣不下体会

胎衣不下指母畜在娩出胎儿后,胎衣在第三产程的生理时限内未能排出,这是牦牛养殖中的一种常见疾病,本人以白牦牛胎衣部分不下、轻症全部不下、重症全部不下为例,运用益母草汤加减治疗20例,对目前白牦牛胎衣不下疾病防控措施和治疗效果进行了探究,以期为白牦牛胎衣不下提供理论依据。     

新型抗生素替代品抗菌肽在畜禽养殖上的应用

抗菌肽又称抗微生物肽,是生物先天性免疫的重要组成成份,具有广谱抗菌作用并且不易产生耐药性。抗菌肽作为饲料添加剂有促生长、防病以及可提高机体免疫力的作用;作为兽药可治疗各类细菌性、病毒性疾病以及各类寄生虫疾病;抗菌肽在畜禽养殖上的功能正在逐渐被开发,有望被开发成为抗生素替代品而广泛应用于畜禽养殖业。本文就家畜来源的抗菌肽从结构、用途和作用机制以及在畜牧兽医领域中的应用进行系统的分析与讨论。