基于数字照片解析的黄瓜叶片面积测定及效果分析

为快速测定黄瓜叶片面积,开发了基于数字照片解析技术的叶片面积测定法,通过与坐标纸法、复印比重法对比,分析数字照片解析法的准确性与稳定性。结果表明：数字照片解析法与坐标纸法测定的结果无显著差异（配对检验P(p＞|t|)=0.872＞0.05）,复印比重法测定的结果较坐标纸法显著偏高。数字照片解析法较坐标纸法更为便捷、准确,可作为一种新的叶面积测定法推广使用。     

一种简单高效的聚丙烯酰胺凝胶DNA回收方法

本文介绍了一种简单的聚丙烯酰胺凝胶DNA回收方法（直取法）,通过与传统煮沸法比较以证明直取法回收聚丙烯酰胺凝胶DNA的高效性。以木薯cDNA为材料,将直取法与常规煮沸法从实验方法、杂带污染率以及回收扩增率三个方面进行比较。实验结果表明：直取法较煮沸法简单、快速;煮沸法的杂带污染率为59.09%,直取法为43.08%;煮沸法的平均产物量约为40 ng/μL,直取法约为60 ng/μL。研究结果表明,直取法相对煮沸法简单,快速,扩增效率高,杂带污染几率小。     

板栗子叶总RNA提取方法研究?

为了获得板栗子叶总RNA提取的理想方法,为后续的分子生物学研究打下基础,以板栗幼果子叶为材料,采用改良CTAB法、硼砂-CTAB法、酚-SDS法、皂土法和2种植物总RNA提取试剂盒共6种方法提取板栗子叶总RNA。结果表明,除硼砂-CTAB法外,其他5种方法均能获得板栗子叶总RNA。但是,与酚-SDS法、皂土法和2种植物总RNA提取试剂盒相比较,改良CTAB法提取的板栗子叶总RNA完整性好,纯度高,且无DNA污染,能够满足cDNA-AFLP等分子生物学研究的要求。     

利用基因型值构建宁夏粳稻核心种质的方法

以250份宁夏粳稻为材料,根据7个农艺性状的遗传多样性,研究粳稻核心种质的构建方法.采用混合线性模型统计分析方法无偏预测材料各性状的基因型值,根据2种遗传距离(马氏距离和欧氏距离),3种取样方法(多次聚类随机取样法、多次聚类优先取样法、多次聚类偏离度取样法),8种聚类方法(最短距离法、最长距离法、中间距离法、重心法、类平均法、可变类平均法、可变法、离差平方和法),用均值、方差、极差、变异系数及秩次评价不同遗传距离、取样方法、聚类方法的优劣.结果表明,马氏距离优于欧氏距离;偏离度取样法优于优先取样法和随机取样法,聚类方法最好的是最短距离法、可变类平均法,适宜取样比例是15％.用马氏距离、偏离度取样法、15％取样比例、最短距离聚类法构建了37份宁夏粳稻核心种质.     

燕麦中β-葡聚糖的含量及相对分子质量测定方法的研究进展

阐述了燕麦中β-葡聚糖的含量及相对分子质量测定方法的国内外研究动态和研究成果,其中包括对β-葡聚糖含量的测定方法(酶测定法、荧光法、高效液相色谱法、刚果红分光光度比色法)的研究,以及对β-葡聚糖相对分子质量测定方法(高效凝胶色谱法、凝胶色谱法、高效液相色谱法)的研究。     

Study on Analysis Methods for Rock Slope FoundationStability of the Highrise Building

Based on the practical project a study on analysis methods for rock slope foundation stability of highrise-building project criterion transfer coefficient method, rotation limit equilibrium method (Sweden slices method) and finite element method was carried out.The authors found that safety factor of criterion transfer coefficient method is larger than that of rotation limit equilibrium method, but is similar to the results of finite element method. So, when rockslope foundation stability of highrise building is to be analysed,the rotation limit equilibrium method is suggested to use.     

秸秆纤维素提取方法比较研究

目的:意在找出适合秸秆纤维素提取的方法。[方法]:分别采用氢氧化钠-醋酸-亚氯酸钠-丙酮法、高压蒸煮法、硝酸-氢氧化钠法、氢氧化钠-氢氧化钠法、硝酸-乙醇法、过氧酸体系法等方法提取秸秆纤维素,采用重铬酸钾-硫酸亚铁铵法测定纤维素的含量,以纤维素含量与所需时间为评价指标,选出最适合提取玉米秸秆纤维素的方法。[结论]：高压蒸煮法和硝酸-乙醇法较优越,具有操作简单、速度快捷、提取率较高的优点。高压蒸煮法处理量大,适合工业生产需要,硝酸-乙醇法提取的纤维素量少,但其含量高,适用于科研机构及相关部门。     

Optimization Method of Turbine Impeller''s Stress and Deformation Calculation

Taking into the feature of computer computing method, using Stress solution of elasticity theory and according to different method of solving of system of second order ordinary differential equations , this article provides two numerical value computing method as for the stress and deformation of the turbine impeller which is within the scope of elasticity . One combines the initial value computing method of system of ordinary differential equations (Runge-Kutta Method) with optimization method, another combines the boundary value computing method of system of ordinary differential equations difference method-with optimization method and three points interpolation method . The proposed method can eliminate the deficiencies of Secondary Calculation method and is particularly suited for programmable computer-based solution . The sample show that results gotten by two methods is nearly equal to the precise results,so they are practicable. They completes the quick and precise calculating of stress and deformation . They have some general meaning , large commonality and the project employing value.     

双波长分光光度法测定土壤硝态氮的可行性研究

为了探寻简单、快速的土壤硝态氮测定方法,以指导农业生产氮肥施用,对双波长长法测定土壤硝态氮的方法进行了探讨,并与广泛应用的反射仪法和流动分析仪测定方法进行了比较,结果表明双波长分光光度法与反射仪测定和流动分析仪测定方法高度相关,操作简便、准确,可用于土壤硝态氮的测定。     

Optimal calculation method of stabilizing force for anti slide piles

A new efficient method named matrix method is presented based on the traditional calculation methods of stabilizing force. The method is derived from the thrust force method and designed to address the deficiency of traditional method. It cannot only get the best pile location with minimum stabilizing force when is applied to the design calculation of single row anti slide piles, but also be applied to the optimal calculation of multi row anti slide piles. The cases comparing results show the method is more reasonable. For major projects of landslide control, the combination of matrix method and finite element method can make the result safer and more accurate.     

THE GENERALIZED NEWTON ITERATIVE PROCEDURE

We suggest a class of generalized Newton method in this paper. As special exampls, it contains the usual Chebyshev method, tangent-hypobolic method and steepest descent method.     

AN OPTIMIZATION METHOD FOR SOLVING THE BOUNDARY WALUE QUESTION OF DIFFERENTIAL EQUATIONS

This paper presents an optimization method for solving the boundary value question of a differential equation on the basis of the trial-and-error method and the optimization method. As compared with the differential method, this method has some advantages such as quiker velocity at count, higher precision(especially at the boundary points) and can be widely used.     

适合双向电泳的植物全蛋白提取方法比较

样品处理是双向电泳实验最基础也是最关键的环节之一,样品制备成功与否直接关系到双向电泳图谱的质量和蛋白质组信息的完整性。应用于双向电泳中提取植物全蛋白的常用方法有：TCA-丙酮沉淀法、Tris-HC1法、酚法、Trizol沉淀法、Tris-丙酮-酚法、尿素/硫脲提取法。不同的方法适合不同特征的植物样品,比较了用于双向电泳的六种植物全蛋白提取方法的优缺点,并对每种方法适用的植物组织作了简要概述。     

脂质体制备方法的研究进展

脂质体是磷脂自聚集而形成的双分子层结构,作为一种重要载体,具有保护药物、提高药效、减少制剂对机体的毒性和提高药物的靶向性等方面的优越性。概述了薄膜蒸发法、高压乳匀法、逆向蒸发法、乙醇注入法及改良乙醇注入法、微乳冷却法、冻融法和冷冻干燥法等几种脂质体的制备方法,以及脂质体的评价指标。     

皱边石杉内生真菌DNA快速有效提取的研究

为了选择一种省时省力、简单有效的皱边石杉内生真菌菌丝体总DNA提取方法,采用氯化苄法提取其总DNA,对比研究石英砂研磨与超声波振荡2种破壁方式对其DNA的提取效能。结果表明,采用石英砂研磨法提取的DNA,其点样孔附近残余杂质较多、拖尾现象严重;超声波破壁法提取的DNA质量总体较好,DNA条带在23.1 kb处集中,多糖、蛋白质等杂质污染少,拖尾现象轻微。比较而言,超声波破壁法可快捷、高效提取皱边石杉内生真菌菌丝体DNA,适于植物内生真菌DNA大规模快速提取,尤其对质地坚硬的菌落较石英砂研磨更有效。提取的DNA经琼脂糖凝胶电泳、ITS序列扩增,表明超声波破壁法提取的DNA与石英砂研磨法一样,均可获得良好的PCR扩增结果。     

枳壳基因组DNA提取方法的比较研究

以枳壳（酸橙）叶片为试验材料,分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA,并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。结果表明,5种方法中SDS法提取的DNA浓度最高（平均为900 ng/ul）,纯度最好（OD260/OD280平均值为1.90）,且SSR-PCR扩增结果与紫外分光光度计和凝胶电泳检测结果基本一致。因此,SDS法是枳壳DNA最佳提取方法,可用于分子检测试验。     

Strictly Analytic Calculation to the Renormalized Finite Quantity of the Photon Loop(Chain) Propagator

In the minimum electromagnetism coupling model of interaction between photon and electron(antielectron),Feynman convergence integral method is often used to deal with the finite quantity of one-loop correction scheme.After analyzing,discussing and researching the method,a new method is found.That is the great moment integral limitation method.Through the method,the relative result is obtained.     

食品中乳酸菌含量倾注法与涂布法结果差异性研究

选取不同品种含有乳酸菌的样品,分别使用倾注法和涂布法进行检测,比较所得结果有无差异性。同时分析了倾注法和涂布法的优缺点。     

A HIERARCHICAL CONTROL METHOD FOR REACTIVE POWER OPTIMIZATION IN LARGE-SCALED INTERCONNECTED POWER SYSTEMS

This paper presents a new hierarchical and coordinative control method for reactive power optimization in large-scale interconnected power systems. It combines the interconnection forecast method for hierarchical control in large-scale steady state industrial systems with the incremental linear programming method for reactive power optimization in real time. The results of applying the method to power system examples show that the method not only solves the problem of high dimension in order, but also keeps the advantages of the incremental linear programming method. The proposed method is also suitable for the security analysis of reactive power correction in large-scale interconnected power systems.     

有机农业生产中的有害生物管理技术

综合介绍了有机生产中控制有害生物的技术措施,主要有:生态学技术措施、农业措施、物理措施以及生物药剂防治措施等,为有机农业生产中有害生物的管理提供了思路和方法。