共查询到20条相似文献，搜索用时 0 毫秒
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CAO Kai-yuan DAI Shu-qin XU Lin YUAN Guang-qing HUANG Xiao-rong QIU Shao-peng GUO Lin-jie 《园艺学报》2004,20(6):1009-1012
AIM: To obtain eukaryotic expression vector of Chinese prostate-specific membrane antigen. METHODS: Chinese prostate-specific membrane antigen (PSMA) cDNA was amplified by RT-PCR from prostate cancer tissues, then cloned into eukaryotic expression vector pcDNA3.0 and sequenced. RESULTS: Seven bases in Chinese PSMA cDNA sequence were found different from those reported by Israeli, which lead to two different amino acids. CONCLUSION: We have obtained the PSMA cDNA, and the recombinant eukaryotic expression vector was successfully constructed. The study lays foundation for DCs vaccine modified by PSMA gene for the treatment of prostate neoplasms. 相似文献
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AIM: To investigate whether homocysteine (HCY) induce the expression of macrophage inflammatory protein-1α(MIP-1α)in cultured THP-1 monocytes. METHODS: After exposure of THP-1 monocytes to HCY at increasing concentrations (0.05,0.1 and 0.2 mmol/L) for 8 h, or at 0.1 mmol/L of HCY for different incubation times (4, 8 and 16 h), the expressions of MIP-1α mRNA and protein were determined by RT-PCR and immunocytochemistry, respectively. RESULTS: RT-PCR showed that the expression of MIP-1α mRNA increased with the concentrations of HCY compared with the control group. Meanwhile, after the treatment of 0.1 mmol/L HCY to the cells for different times, the MIP-1α mRNA expression increased at 4 h, peaked at 8 h, and then decreased at 16 h. The authenticity of RT-PCR products was confirmed by DNA sequencing. Image analysis of Immunocytochemistry assay showed the expression of MIP-1α protein in experimental groups increased in a dose- and time-dependent manner(P＜0.01). CONCLUSIONS: HCY induced monocytes to express MIP-1α mRNA and protein. 相似文献
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PI Rong-biao YIN Wei SU Xing-wen SU Tao QIU Peng-xin ZHENG Su-qiu YAN Guang-mei 《园艺学报》2004,20(9):1557-1562
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AIM:To study the effect of IFN-γ inhalation on the anti-infection ability of the lungs in the immunocompromised host. METHODS:The immunological factors in the immunocompromised rats and the immunocompromised rats administrated IFN-γ via aerosol were investigated after 1, 3, 7 days when they were injected Candida albicans via tracheal. The Canidda albicans count of the left lung was also determined after 7 days when injecting pathogen. RESULTS:The Canidda albicans count of the left lung in IFN-γ group was significantly less than that of control group. The phagocyting and bactericidal percentages, Ia antigen expression percentages, the levels of TNF-α, IL-1β and IL-6 in the culture supernatant of the AM, the activity of IFN-γ and TNF-α in BALF (except the TNF-α on 7 th day) in IFN-γ group were markedly higher than those in control group. The expression of IFN-γ and IL-1β pulmonary tissues in IFN-γ group was higher than that in control group. The expression of TNF-α in IFN-γ group was less than that in control group. The expression of IL-6 was no changes between two groups. The levels of IFN-γ, IL-1β and IL-6 in the blood (except IL-1β on 3 rd day), and the killing ability of the lymphocytes in blood had no difference between two groups. CONCLUSION:Administration of IFN-γ via aerosol obviously enhanced the anti-infection ability of the lungs in the immunocompromised host, but has no influence on the whole body cellular immunity. 相似文献
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AIM:To investigate the effects of pyrrolidine dithiocaramate (PDTC) and vitamin C (Vit C) on the intercellular adhesion molecule 1 (ICAM-1) gene expression and the superoxide dismutase (Mn-SOD, Cu, Zn-SOD) gene expression in a rat model of acute pancreatitis (AP). METHODS:Rat AP model was induced by retrograde injection of 3% sodium taurocholate in the bilio-pancreatic duct. The AP rats were divided into three groups: the normal saline (NS) group, the PDTC group and the Vit C group. The three groups were separately administered NS, PDTC and Vit C at 10 min and 5 h after operation. Rat sham operation (SO) group was subjected to laparotomy only with no further treatment. The levels of ICAM expression, Mn-SOD and Cu, Zn-SOD expression in pancreas and liver were determined by RT-PCR at 1, 5 and 10 h after the induction of AP. RESULTS:Comparing with SO group, the NS group showed a higher expression of ICAM-1 and a lower expression of Mn-SOD both in pancreas and in liver, and a lower expression of Cu, Zn-SOD in pancreas. PDTC treatment reduced the ICAM-1 expression and increased the Mn-SOD and Cu, Zn-SOD expression to a certain degree comparing with NS group, especially at 5 h. Vit C treatment also increased the Mn-SOD and Cu, Zn-SOD expression. But no obvious suppressive effect on ICAM-1 expression was observed in Vit C group.CONCLUSIONS:PDTC and Vit C have the effects of enhancing Mn-SOD and Cu, Zn-SOD expression.PDT C has an effect of inhibiting ICAM-1 expression in the pancreas and liver of rats with AP, whereas Vit C dose not.The results suggest that one of the mechanisms of PDTC and Vit C treat ing AP not only anti-oxidate directly by themselves, but also enhance Mn-SOD and Cu, Zn-SOD expression, and that the anti-inflammatory ef ect of PDTC is involved in the suppression of ICAM-1 expression. 相似文献
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HUANG Xiang-hua ZHANG Xiang-hong YAN Xia YIN Gui-ran LI Yue-hong TAN Yan-wei WANG Jun-ling WANG Feng-rong 《园艺学报》2002,18(2):169-171
AIM:To explore the putative effects of sterigmatocystin (ST) on human help T lymphocyte(Th1)function. METHODS:The effects of ST on interferon-γ(IFN-γ)secretion of human peripheral blood mononuclear cells(HPBMc) in vitro were determined with ELISA method. RESULTS:The effects of ST on IFN-γ secretion of HPBMc in vitro were closely dependent on ST concentrations. ST at relatively lower concentrations (0.03125-0.12500 mg/L) showed inhibiting effects on IFN-γ secretion. While, stimulating effects could be found when ST concentration was above 0.25mg/L. The highest level was seen in ST 1 mg/L group (P＜0.05)。At concentration ranging from 0.25 mg/L to 1 mg/L, a positive dose- effects correlation was found between ST concentration and IFN-γ secretion (r=0.492, P＜0.01). Time-effects analysis from 1 h to 64 h after ST treatment (1 mg/L)showed that the effects of ST on IFN-γ secretion varied as the changes of treatment times. An inhibiting effect on IFN-γ secretion of HPBMc 4 h and 8 h after ST treatment was found (8 h, P＜0.05). As the treatment time prolonged from 16 h, IFN-γ level gradually increased (32 h, P<0.05). There was a positive correlation between treatment time of ST and IFN-γ level of HPBMc in vitro from 16 h to 64 h after ST treatment (r=0.736, P＜0.01).CONCLUSION:The effects of ST on IFN-γ secretion of HPBMc in vitro closely depended on concentration and treatment time of ST. Generally, inhibiting effects were found at relatively lower ST concentration and shorter treatment period, while stimulation effects could be seen at relatively higher ST concentration and longer ST treatment time period. 相似文献
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Thayne Montague Edward W. Hellman David Appel Michael Krawitzky 《International Journal of Fruit Science》2016,16(2):135-149
An objective of this study was to investigate rooting success of grape cuttings propagated from vines symptomatic of Pierce’s disease. Additional objectives were to assess if rooted cuttings could survive and produce viable plants, and determine if Xylella fastidiosa (causal agent of Pierce’s disease) could be found in rooted cuttings. In Jan. 2008, cuttings were taken from symptomatic and asymptomatic ‘Merlot’ and ‘Cabernet Sauvignon’ grapevines growing in the Hill Country and Gulf Coast regions of Texas. Six weeks after cuttings were propagated, each cutting was uprooted and evaluated for rooting and infection parameters. Cuttings were then planted in containers and held in the greenhouse to evaluate survivability. To confirm the presence of X. fastidiosa, propagated cuttings were tested by real-time polymerase chain reaction. Data indicate several rooted cuttings tested positive for X. fastidiosa and appeared viable and healthy. Therefore, vines infected with X. fastidiosa have the ability to produce asexually propagated cuttings, and potentially contaminate non-infected vineyards. 相似文献
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AIM:To clarify if interferon-γ(IFN-γ), tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)can induce apoptosis of human airway smooth muscle cells (ASMCs) in vitro.METHODS:Human ASMCs were isolated and cultured in DMEM containing 10% fetal bovine serum. Passage 4-6 cell was used in the experiment. IFN-γ,TNF-α and IL-1β, were used separately or together in the treatment of human ASMCs. The effects of IFN-γ,TNF-α and IL-1β on the growth of the cells was detected by MTT method at the hour 0,24,48 and 72. Light microscopy and electron microscopy were used to examine the morphological change. DNA fragmentation was analyzed by agarose gel electrophoresis. SP immunohistological staing method was performed to detect the change of expressions of p 53, bcl- 2 and bax gene. The apoptosis cell percentage were detected by in situ end labeling technique (TUNEL)of fragmental DNA. RESULTS:(1)IFN-γ or IFN-γ together with TNF-α and IL-1β decreased the number of viable cells in a time dependent manner. (2) Light and electron microscopic examination showed cell shrinkage, membrane blebbing, nuclear contraction, chromatin condensation and nuclear fragmentation in human ASMCs. (3) Agarose gel electrophoresis showed a characteristic\"ladder\"of DNA bands representing integer multiples of the internucleosomal fragments (about 180-200 bp) in cytokine cotreated human ASMCs. (4)The expression of p 53 and bax gene in cytokine cotreated group was significantly higher than in control group, but the expression of bcl-2 gene was lower than in control group. (5)Stimultaneous treatment with IFN-γ(4×105 U/L),TNF-α(4×105 U/L)and /or IL-1β (10×104 U/L) induced apoptosis of human ASMCs. Apoptotic index of human ASMCs in cytokine co-treated group was significantly higher than in control group (P＜0.01).CONCLUSION:Stimultaneous treatment with IFN-γ,TNF-α and /or IL-1β induced apoptosis of human ASMCs. These immune cytokines may play an important role in airway remodeling of asthma and of chronic obstructive pulmonary disease. 相似文献
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AIM:To study the variety of cytokines in severe bacterial pneumonia of Sprague Dawleg (SD) rats. METHODS:A total of 60 SD rats were randomly divided into three groups: model Ⅰ group (n=24), model Ⅱ group (n=24), and control group (n=12). Rats in the model Ⅰ group and the model Ⅱ group were intratracheally instilled with suspension of klebsiella pneumoniae at different doses. Rats in the control group were intratracheally injected with 1 mL saline. On the 2nd, 4th and 6th day after intratracheal instillation, 1/3 rats in each group were killed to determine the concentration of IFN-γ, IL-6 and TNF-α in blood. RESULTS:The levels of IL-6 and TNF-α in model groups were higher than those in control group, while the level of IFN-γ was lower. The change of cytokines was more significant in the model Ⅱ group (severe pneumonia) than those in the model Ⅰ group. CONCLUSION:The cytokines we studied may play an important role in the pathogenesis of severe pneumonia. The change of cytokines is more significant in severe pneumonia than those in common pneumonia. 相似文献
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AIM: To study the effects of dexamethasone (DXM) on intracellular expression of TH1/TH2 cytokines and the mechanism of that during the development of asthma. METHODS: Eighteen BALB/c mice were divided into 3 groups at random: control group, asthma group, and DXM treated group, with 6 mice in each. The expressions of T-box expressed in T cells (T-bet) and GATA-binding protein-3 (GATA-3) in lung tissue were detected by Western blotting. The expressions of intracellular cytokines interleukin-4 and interferon-γ in CD4+ T cell were measured by flowcytometry.RESULTS: The results of flow cytometry indicated that the ratio of intracellular cytokines IL-4/IFN-γ in CD4+ T cells in asthma group was much higher than that in control group (P<0.01), the ratio of intracellular IL-4/IFN-γ in T cells in DXM group was lower than that in asthma group significantly (P<0.01). The expression of T-bet in lung tissue in asthma group was lower than that in control group significantly (P<0.01), while GATA-3 was higher than that in control group significantly (P<0.01). The expressions of T-bet and GATA-3 in DXM group were much lower than those in asthma group (P<0.01), but the decreased degree of GATA-3 was more than that of T-bet. CONCLUSION: With pathological process of asthma, to reverse the ratio of IL-4/IFN-γ in CD4+ T cell by regulating T-bet and GATA-3 expression can improve the inflammatory reaction and may be one of the mechanisms of DXM in treating asthma. 相似文献
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AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps. 相似文献
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XIONG Li-xia LI Wen-lin CAI Zhen-yu ZHOU Ying XIONG Jun-ping ZHAO Lin HE Xiao-yan SHI Xiao-yu 《园艺学报》2013,29(3):504-508
AIM：To investigate the suppressive effect of interferon γ (IFN-γ) on fibrosis induced by interleukin 13 (IL-13) in fibroblasts. METHODS：The fibroblasts were divided into IFN-γ (4×105U/L) group, IL-13 (100 μg/L) group, IFN-γ+IL-13 group and blank control group. At 24 h, 48 h and 72 h, the secreted collagen from fibroblasts was measured by hydroxyproline release assay. The mRNA expression of collagen type I α1 (Col1A1) in fibroblasts was examined by RT-PCR. The protein level of collagen type I synthesized in fibroblasts was analyzed by Western blotting. RESULTS：IFN-γ at 4×105U/ L significantly inhibited the proliferation of fibroblasts and down-regulated Col1A1 mRNA and cellular collagen. The mRNA expression of Col1A1 and the protein level of collagen type I in IFN-γ group were lower than those in blank control group at 48 h and 72 h. At 72 h, the mRNA expression of Col1A1 and the protein level of collagen type I in IL-13 group were substantially higher than those in blank control group, those in IFN-γ + IL-13 group were remarkable lower than those in blank control group, and those in IFN-γ group were also lower than those in blank control group. CONCLUSION：IFN-γ inhibits the fibrotic effect of IL-13 in fibroblasts. 相似文献
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苹果中Ty1-copia型逆转座子逆转录酶序列的克隆及分析 总被引：3，自引：0，他引：3
利用苹果叶片为材料,通过PCR扩增、克隆研究了苹果中Ty1-copia型逆转座子的逆转录酶序列。结果表明:(1)逆转座子在1个砧木及7个品种中都能检测到,说明逆转座子在苹果砧木及不同种质中普遍存在。(2)克隆、分析了嘎拉品种中23条逆转座子的逆转录酶序列,分析表明同一基因组中克隆到的逆转录酶序列存在高度的异质性,具体表现为23条逆转录酶序列核苷酸序列长度变化范围是247-279bp,其长度相差32bp,主要表现为缺失突变;其中6条序列中存在1-4个程度不同的终止密码子突变;氨基酸序列同源性的变化范围为21%-98.9%。(3)将23条序列与已发表的不同物种同一类型逆转座子的逆转录酶序列进行聚类分析,表明苹果的Ty1-copia型逆转座子与甜菜、水稻、马铃薯、燕麦及挪威云杉等物种的同类型逆转座子可能有相同的起源。 相似文献
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羧甲基茯苓多糖促诱生细胞因子的研究 总被引：1，自引：1，他引：1
羧甲基茯苓多糖（Carboxymethylpachymaran,CMP）12.5～100μg/ml对Nama-lwa类淋巴细胞、S801和S7811白血病细胞促诱生干扰素-α（IFN-α）效价比常规诱生高10～20倍;10μg/ml对人血淋巴细胞促诱生干扰素-γ（IFN-γ）效价比常规添生高8倍;100μg/ml对人血淋巴细胞促诱生白细胞介素-2（IL-2）效价可以达到2500IU/ml;20～100μg/ml可以刺激人外伤脾细胞产生淋巴毒素（Lymphotoxin,LT）及新抗癌淋巴因子—一白细胞调节素（Leukoregulin,LR）。 相似文献
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以杏鲍菇枯萎病菌侧耳泛菌(Pantoea pleuroti)为试材,采用PCR引物设计的方法,通过分析P.pleuroti基因组的测序结果,设计合成并筛选出可检测P.pleuroti的引物,并研究其检测效果,以期建立P.pleuroti的高效PCR检测体系,并为科学防控杏鲍菇枯萎病提供分子基础。结果表明:K3143F/R和K37F/R 2对引物特异性强,仅P.pleuroti基因组DNA作为模板时,PCR扩增产物分别呈现1条660 bp和1条666 bp的特异性条带,15株Pantoea属细菌和3株食用菌常见病原菌的基因组DNA及阴性对照作为模板扩增产物均无条带;基于这2对引物建立的检测体系均不受杏鲍菇组织液的干扰,灵敏度高,可检测出最低3.6 pg·μL-1的P.pleuroti基因组DNA;应用这2种体系对接种P.pleuroti 48 h后的杏鲍菇样品进行了检测,最低可检测出子实体内100 cfu的杏鲍菇枯萎病菌;此外,整体上,引物K3143F/R比K37F/R的检测效率更高。 相似文献