共查询到20条相似文献,搜索用时 31 毫秒
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HTLV-I tax induces cellular proteins that activate the kappa B element in the IL-2 receptor alpha gene 总被引:91,自引:0,他引:91
D W Ballard E B?hnlein J W Lowenthal Y Wano B R Franza W C Greene 《Science (New York, N.Y.)》1988,241(4873):1652-1655
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Kappa B-specific DNA binding proteins: role in the regulation of human interleukin-2 gene expression 总被引:72,自引:0,他引:72
B Hoyos D W Ballard E B?hnlein M Siekevitz W C Greene 《Science (New York, N.Y.)》1989,244(4903):457-460
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Sequence-specific binding of human Ets-1 to the T cell receptor alpha gene enhancer 总被引:48,自引:0,他引:48
I C Ho N K Bhat L R Gottschalk T Lindsten C B Thompson T S Papas J M Leiden 《Science (New York, N.Y.)》1990,250(4982):814-818
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Identification of a putative regulator of early T cell activation genes 总被引:98,自引:0,他引:98
J P Shaw P J Utz D B Durand J J Toole E A Emmel G R Crabtree 《Science (New York, N.Y.)》1988,241(4862):202-205
Molecules involved in the antigen receptor-dependent regulation of early T cell activation genes were investigated with the use of functional sequences of the T cell activation-specific enhancer of interleukin-2 (IL-2). One of these sequences forms a protein complex, NFAT-1, specifically with nuclear extracts of activated T cells. This complex appeared 10 to 25 minutes before the activation of the IL-2 gene. Studies with inhibitors of protein synthesis indicated that the time of synthesis of the activator of the IL-2 gene in Jurkat T cells corresponds to the time of appearance of NFAT-1. NFAT-1, or a very similar protein, bound functional sequences of the long terminal repeat (LTR) of the human immunodeficiency virus type 1; the LTR of this virus is known to be stimulated during early T cell activation. The binding site for this complex activated a linked promoter after transfection into antigen receptor-activated T cells but not other cell types. These characteristics suggest that NFAT-1 transmits signals initiated at the T cell antigen receptor. 相似文献
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Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B 总被引:117,自引:0,他引:117
S M Ruben P J Dillon R Schreck T Henkel C H Chen M Maher P A Baeuerle C A Rosen 《Science (New York, N.Y.)》1991,251(5000):1490-1493
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N Davis S Ghosh D L Simmons P Tempst H C Liou D Baltimore H R Bose 《Science (New York, N.Y.)》1991,253(5025):1268-1271
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K Muegge T M Williams J Kant M Karin R Chiu A Schmidt U Siebenlist H A Young S K Durum 《Science (New York, N.Y.)》1989,246(4927):249-251
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Interleukin-2 receptor beta chain gene: generation of three receptor forms by cloned human alpha and beta chain cDNA's 总被引:101,自引:0,他引:101
M Hatakeyama M Tsudo S Minamoto T Kono T Doi T Miyata M Miyasaka T Taniguchi 《Science (New York, N.Y.)》1989,244(4904):551-556
Interleukin-2 (IL-2) binds to two distinct receptor molecules, the IL-2 receptor alpha (IL-2R alpha, p55) chain and the newly identified IL-2 receptor beta (IL-2R beta, p70-75) chain. The cDNA encoding the human IL-2R beta chain has now been isolated. The overall primary structure of the IL-2R beta chain shows no apparent homology to other known receptors. Unlike the IL-2R alpha chain, the IL-2R beta chain has a large cytoplasmic region in which a functional domain (or domains) mediating an intracellular signal transduction pathway (or pathways) may be embodied. The cDNA-encoded beta chain binds and internalizes IL-2 when expressed on T lymphoid cells but not fibroblast cells. Furthermore, the cDNA gives rise to the generation of high-affinity IL-2 receptor when co-expressed with the IL-2R alpha chain cDNA. 相似文献
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Li B Yu H Zheng W Voll R Na S Roberts AW Williams DA Davis RJ Ghosh S Flavell RA 《Science (New York, N.Y.)》2000,288(5474):2219-2222
T helper 1 (TH1) cells mediate cellular immunity, whereas TH2 cells potentiate antiparasite and humoral immunity. We used a complementary DNA subtraction method, representational display analysis, to show that the small guanosine triphosphatase Rac2 is expressed selectively in murine TH1 cells. Rac induces the interferon-gamma (IFN-gamma) promoter through cooperative activation of the nuclear factor kappa B and p38 mitogen-activated protein kinase pathways. Tetracycline-regulated transgenic mice expressing constitutively active Rac2 in T cells exhibited enhanced IFN-gamma production. Dominant-negative Rac inhibited IFN-gamma production in murine T cells. Moreover, T cells from Rac2-/- mice showed decreased IFN-gamma production under TH1 conditions in vitro. Thus, Rac2 activates TH1-specific signaling and IFN-gamma gene expression. 相似文献
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I kappa B: a specific inhibitor of the NF-kappa B transcription factor 总被引:273,自引:0,他引:273
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Cyclosporin A specifically inhibits function of nuclear proteins involved in T cell activation 总被引:79,自引:0,他引:79
E A Emmel C L Verweij D B Durand K M Higgins E Lacy G R Crabtree 《Science (New York, N.Y.)》1989,246(4937):1617-1620
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Generation and analysis of interleukin-4 deficient mice 总被引:117,自引:0,他引:117
Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgG1 and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo. 相似文献
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T M Williams D Moolten J Burlein J Romano R Bhaerman A Godillot M Mellon F J Rauscher J A Kant 《Science (New York, N.Y.)》1991,254(5039):1791-1794
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为了研究鸡B细胞膜免疫球蛋白(mIg)的功能,深入了解鸡免疫系统抗体基因的特点,从鸡法氏囊B细胞cDNA中扩增出Igλ轻链基因,对其核苷酸序列和氨基酸序列进行了分析和比较。该基因cDNA全长873bp,编码含226个氨基酸的Igλ轻链,N-端21个氨基酸构成轻链信号肽,随后是2个Ig样结构域。运用融合PCR的方法将该基因与牛IgG Fc受体γRⅡ跨膜区序列嵌合形成重组跨膜分子,构建真核表达载体pcDNA-λR2T,转染COS7细胞,荧光抗体染色及流式细胞术检测到重组鸡Igλ轻链在细胞膜上的表达。所扩增的鸡Igλ轻链基因以及构建表达于细胞膜上的重组鸡Igλ轻链跨膜分子,为研究鸡免疫系统中的抗体轻链基因,探索Igλ轻链和B细胞膜免疫球蛋白的功能奠定技术基础。 相似文献