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After electrophoresis on cellulose acetate, two haemoglobin phenotypes were detected in Baloochi and Kordi breeds: AA and AB phenotypes. AA was commonest in two breeds. The incidence of type AB haemoglobin in Baloochi and Kordi breeds was 26.5% (9/34) and 9.5% (2/21), respectively. BB phenotype was not seen in Baloochi and Kordi breeds. In sheep with AB phenotype, haemoglobin B was dominant. The mean +/- SD of the two kinds of haemoglobin in sheep with AB phenotype were haemoglobin B percentage 60.5% +/- 9.04%, haemoglobin B absolute 73.84 +/- 5.44 g/L, haemoglobin A percentage 39.5% +/- 9.04%, haemoglobin A absolute 32.88 +/- 2.89 g/L. There were no significant differences for total haemoglobin, haematocrit, red blood cell (RBC) number, iron and copper levels between breed, sex and age groups and also between sheep with AA phenotype and AB phenotype. Pearson's method showed significant correlations for total haemoglobin with packed cell volume (PCV), RBC number, copper concentration and RBC number with PCV, copper level and PCV with copper amount and copper concentration with iron level (p<0.05). In the Kordi breed, significant correlations were seen for total haemoglobin with PCV, RBC number, copper concentration and PCV with RBC number and RBC number with copper level and copper concentration with iron amount (p<0.05). In the Baloochi breed, significant correlations were detected for total haemoglobin with PCV, RBC number and PCV with RBC number (p<0.05). 相似文献
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试验对海拔 3 0 0 0m的藏南谷地林芝引入家兔进行了部分生理指标的对比分析。测定了呼吸频率、心率、生理体温、血清蛋白质含量和血红蛋白含量。统计分析表明 :所测定项目的结果在 3品种兔之间均无明显差异 (P >0 0 5) ;但血红蛋白电泳分析 :3品种兔均呈现出A、A2 ,A33个区带 ,发现A3带以青紫兰兔最低 ,与其他 2品种兔差异极显著 (P <0 0 1 ) ;加里福尼亚兔A3带最高 ,显著高于中国白兔 (P <0 0 5)及青紫兰兔 (P <0 0 1 )。血清蛋白质电泳分析表明 :高原家兔的血清白蛋白均明显高于文献报道的低海拔饲养的新西兰白兔 ,血清总蛋白及其球蛋白含量明显下降 相似文献
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Blood volume determinations were performed in 5 anesthetized gopher snakes (Pituophis melanoleucus catenifer) by means of a 51Cr-labeled red blood cell (RBC) method. The mean blood volume was 52.8 ml/kg of body weight (+/- 6.21 SE). Previous blood volume measurements have not been reported for this species. The RBC survival rate was estimated to be greater than 660 days. The RBC survival rate is long, but it cannot be determined accurately by this method. 相似文献
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M.D. Rubio B.M. Escribano A. Oropesa P. Tovar F.M. Castejn R. Vivo 《Journal of Equine Veterinary Science》1996,16(6):249-253
Possible alterations in the erythrogram and in red blood cell volume indices were studied in ten Andalusian stallions subjected to two different types of exercise (trotting and galloping). For this purpose, the changes in these blood parameters were measured during resting, immediately after the exercise finished, and at 5, 10 and 30 min. of recovery.From the results it was deduced that the red blood cell count (RBC), the hematocrit value (PCV) and the concentration of hemoglobin (HB) increased significantly (p≤0.05) after finishing both types of exercise, with a greater increase with galloping. The return to baseline values was faster for trotting than for galloping. However, the RBC volume indices (mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration) did not undergo any significant changes with either type of exercise. 相似文献
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首次检测了黑麂血液常规和生理生化等23项指标,并与鹿科梅花鹿做了比较。测定结果显示,黑麂的红细胞、血小板、血红蛋白、红细胞比容高于梅花鹿指标,而白细胞、红细胞体积、红细胞平均血红蛋白浓度、平均血红蛋白量、红细胞体积分布宽度指标低于梅花鹿指标。黑麂白细胞分类结果显示,白细胞和单核细胞约占90%;嗜酸性粒细胞和嗜碱性粒细胞所占百分比各占约2%。黑麂血清生化指标测定结果显示,总胆固醇和血糖两项指标高于梅花鹿的指标;甘油三酯(TG)及极低密度脂蛋白(VLDI-C)含量显著高于梅花鹿。检测结果反映了黑麂的机体生理机能,是进行疾病临床诊断和治疗的依据。 相似文献
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果寡糖对固始鸡血液生理指标的影响 总被引:3,自引:0,他引:3
本试验旨在研究不同添加剂量的果寡糖对固始鸡血液生理指标的影响,并确定果寡糖的最佳添加剂量。选用1日龄固始鸡公雏150只,随机分成5个处理组,1个为对照组,其余4个为试验组,对照组不加果寡糖,其他4个组的果寡糖添加水平分别为3、5、7mg/kg和9mg/kg。测定红细胞压积(PCV)、血红蛋白(Hb)含量、红细胞数量、白细胞数量、平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)和平均红细胞血红蛋白浓度(MCHC)等指标。试验结果表明:1)21和42日龄时,果寡糖试验组的红细胞压积、血红蛋白含量、红细胞数量和白细胞数量均显著高于对照组(P<0.05);与对照组相比,试验组的MCH和MCHC均略有升高,并且可以显著提高MCHC(P<0.05);2)日粮中添加5mg/kg果寡糖对提高红细胞压积、血红蛋白含量、红细胞数量、白细胞数量、MCH和MCHC具有最佳效果。 相似文献
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Certain bovine peripheral blood lymphocytes (PBL) and foetal thymocytes were shown to bind autologous and allogeneic red blood cells (RBC). When autologous RBC were treated with dextran, approximately 10% of peripheral blood lymphocytes and about 30% of thymocytes were found to form rosettes. Cells forming autologous rosettes appear to be a population of T-lymphocytes because (1) more rosette formation occurred with thymocytes than with PBL, (2) autologous rosette formation was increased in PBL cultures enriched in T cells and was decreased in cultures depleted of T cells, (3) very few rosette forming cells had surface immunoglobulin and (4) peripheral blood mononuclear cell cultures depleted of monocytes did not show a decreased autologous rosette formation. It appears that the cells forming rosettes with autologous and allogeneic RBC belong to the same sub-population of T-cells. 相似文献
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Some blood physiological indices were determined in wild yak,Datong yak(the crossbreeds of the wild and domestic yak)and Tianzhu Black yak in a year. The red blood cell amount(RBC)and packed cell volume (PCV)of the wild yak and Datong yak were similar but differed largely from that of the Tianzhu Black yak. RBC of the wild yak and Datong yak were larger than that of the Tianzhu Black yak and their RBC varied seasonally.The results indicated that wild yak and Datong yak could effectively adapt to changes in environment by adjusting their physiological indices. 相似文献
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S F Amakiri L O Ngere S K Olusanya B O Ikede 《Tropical animal health and production》1979,11(2):106-112
The blood values of the German Brown, N'Dama and their three-quarter, half and quarter hybrids were studied to ascertain if and how some haematological parameters changed with crossbreeding. It was found that the red blood cell (RBC) and white blood cell (WBC) count and packed cell volume percentage (PCV) were significantly higher (P less than 0.01) in the N'Dama than in the German Brown; the crosses had values which were intermediate as compared to the two parent breeds. Generally, the changes in the mean blood values paralleled changes in the expected genotypes resulting from crossbreeding of the two parent breeds. The tendency of the blood values to change in direct proportion to the degree of N'Dama contribution was strongest for the WBC, followed by the PCV and RBC, respectively. A small random sample for leucocyte differential count did not reveal any differences amongst the genotypic groups. 相似文献
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The effects of various concentrations of furosemide and pentoxifylline on equine RBCin vitro were evaluated to facilitate better understanding of the potential effects of these drugs on blood flow properties. Furosemide induced increased mean cell volume (MCV), increased RBC potassium concentration, increased whole blood viscosity, and decreased the RBC filtrability. These data indicate that furosemide may block the RBC membrane transport pathways resulting in potassium and water retention. The increase in size and the resultant decrease in the surface-area-to-volume ratio may have caused the impaired RBC filtrability and increased blood viscosity. Pentoxifylline improved RBC filtrability without changing the RBC size or the potassium or chloride concentrations, suggesting that pentoxifylline may increase the deformability of the RBC membrane. The study indicated that pentoxifylline has potential therapeutic applications for improving microvascular blood flow but that furosemide may have adverse effects on blood flow.Abbreviations cP
centipoise
- EIPH
exercise-induced pulmonary haemorrhage
- MCV
mean cell volume
- Na-K-2Cl
sodium potassium 2-chloride
- PCV
packed cell volume
- RBC
red blood cell 相似文献
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Boisvert AM Tvedten HW Scott MA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1999,28(3):91-96
Hypernatremia in two cats and hyponatremia in a dog were associated with artifactual changes in red blood cell (RBC) indices and hematocrit (HCT) determined on a Bayer H*1 hematology analyzer. The RBC cytograms and histograms revealed a population shifted towards macrocytic, hypochromic RBC in the hypernatremic cats, and towards microcytic, hyperchromic RBC in the hyponatremic dog. Reference intervals for the difference between manual packed cell volume (PCV) and analyzer-derived HCT in normonatremic dogs, cats and horses were established. The difference between PCT and HCT was outside the reference values for all three patients. Quality control measures, such as measuring PCV, and reviewing cytograms and histograms are essential for detecting spurious changes in automated hematology measurements caused by abnormalities in serum sodium concentration and osmolality. 相似文献
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Weiss DJ Kristensen A Papenfuss N 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1993,22(4):117-121
Irregularly speculated red blood cells (IS-RBC) were quantified on fresh blood fixed in glutaraldehyde and were compared to RBC shape changes observed on Wright's-stained blood smears, RBC histograms, and RBC distribution widths (RDW). IS-RBC were infrequently found in healthy control dogs. Twenty dogs with increased IS-RBC were evaluated. The most common clinical diagnoses were lymphosarcoma (seven cases), glomerulonephritis (two cases), hemangiosarcoma (two cases), and chronic liver disease (two cases). Five cases had evidence of disseminated intravascular coagulopathy. In 12 of the 20 cases, keratocytes, schizocytes, and/or acanthocytes were detected in the monolayer area of blood smears. In the other seven cases, keratocytes, schizocytes, and/or acanthocytes were found only in thick areas of the smears. Acanthocytes were the most frequent cell type seen, while schizocytes were absent or present only in low numbers. RBC histograms had a shoulder on the left side of the tracing in six of the 20 cases, suggesting the presence of RBC fragments; however, cases with evidence of platelet aggregation had similar shoulders in RBC histograms. Red cell distribution widths were increased in 12 of the 20 cases with IS-RBC; however, the increase in RDW did not correlate with the presence of schizocytes and was most likely the result of reticulocytosis. This study suggests that quantitative evaluation of RBC shape is a more sensitive method for detection of mild RBC fragmentation when compared to blood smear evaluation, RBC histograms, or RDW. Additionally, acanthocyte-type cells were the most frequent shape change seen in dogs with evidence of RBC fragmentation. 相似文献
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Ogikubo Y Norimatsu M Sasaki Y Yasuda A Saegusa J Tamura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1189-1193
The effect of lipopolysaccharide (LPS) on humoral and cell-mediated immunity was assessed using LPS-sensitive C3H/HeN mice. A single injection of LPS significantly decreased the anti-sheep red blood cells (SRBC) antibody titers, but not the number of anti-SRBC antibody producing spleen cells. In contrast, double LPS injection did not significantly decrease the anti-SRBC titers and even increased the number of anti-SRBC antibody producing spleen cells. Similarly, single LPS injection significantly suppressed the swelling of the footpad, but double LPS injection caused milder suppression. These results suggest that a difference in the level and timing of exposure to LPS may influence the immune response to infection or vaccination. 相似文献
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J Broekman P A Eksteen D W Verwoerd 《The Onderstepoort journal of veterinary research》1977,44(3):151-156
A novel technique, based on cytotoxicity-neutralization, was developed for the in vitro titration of anti-sheep lymphocyte and anti-sheep macrophage sera. The titres obtained for a number of antisera were compared with those found in an agglutination assay. Anti-lymphocyte sera with a high cytotoxicity-neutralization titre very effectively suppressed the number of circulating lymphocytes in the peripheral blood of treated new-born lambs, thus indicating in vivo immunosuppressive activity. 相似文献
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根据氟化钠抑制血液葡萄糖分解,但使红细胞体积缩小,而草酸铵使红细胞体积膨胀的原理,设计和研究出一种氟化钠-草酸铵抗凝剂新配方(1:5.2)。经乳牛、牦牛、绵羊、猪、马和兔血液的试验表明,这种抗凝剂新配方对 PCV 无影响,又能有效地抑制血液葡萄糖分解。 相似文献
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应用红细胞促淋巴细胞转化和促NK细胞活性试验,对不同生理发育时期日本大耳白兔红细胞调控淋巴细胞和NK细胞活性进行研究。结果表明:青年组日本大耳白兔红细胞调控淋巴细胞能力明显高于幼年兔和老年兔(P〈0.01);老年组红细胞调控NK活性能力明显高于青年组和幼年组(P〈0.01),青年组高于幼年组(P〈0.01)。日本大耳白兔红细胞调控淋巴细胞能力在青年时期达到了高峰.随后逐渐降低:而红细胞调控NK活性随年龄增长而显著增强,这提示老年兔的免疫活性细胞较多地处于预激活状态,并对刺激显示良好的免疫应答能力。 相似文献
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Allen AL Myers SL Searcy GP Fretz PB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1998,27(3):93-100
The Hematologic values of 19 equine fetuses between 202 and 238 days gestation were compared with those of their dams. The red blood cell (RBC) count, hemoglobin concentration, hematocrit, and mean corpuscular hemoglobin concentration were significantly lower in fetal blood, while the mean corpuscular volume, mean corpuscular hemoglobin, and red cell distribution width were significantly higher. Mares had a significantly higher nucleated blood cell count than fetuses, and all nucleated cells were leukocytes (WBC). Most WBC in mare blood were segmented neutrophils and lymphocytes. In contrast, over one-half of the nucleated cells in fetal blood were nucleated RBC, and the majority of WBC in fetal blood were lymphocytes. Mares also had significantly higher plasma protein and fibrinogen concentrations than their fetuses. Mild macrocytosis and mild polychromasia were observed in most fetal blood samples, but not in blood samples from mares. All fetal blood contained reticulocytes, and most samples contained Heinz bodies and Howell-Jolly bodies. The results of this study will contribute to the development of hematologic reference values that may be useful in equine fetal research and, possibly, in the diagnosis of equine fetal disease. 相似文献
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Cebulj-Kadunc N Bozic M Kosec M Cestnik V 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2002,49(4):217-221
Haematological parameters [red blood cell count (RBC), white blood cell count (WBC), packed cell volume (PCV), haemoglobin concentration, mean cell volume (MCV), mean cell haemoglobin (MCH) and mean cell haemoglobin concentration (MCHC)] in resting Lipizzan horses were determined for 143 stallions, 104 mares and 25 foals. The mean RBC and WBC values in Lipizzans were in the lower part of the normal range for warm-blooded horses. The mean PCV, MCV and MCH values were higher, but the mean haemoglobin concentration and MCHC values were lower than reported for other warm-blooded horses. In foals, the mean RBC, WBC, PCV, haemoglobin concentration and MCHC values were higher, whereas MCV and MCH were lower than in older animals. Results indicating a significant decrease in WBC (P < 0.01) and an increase in MCV, MCH and MCHC (P < 0.05) with increasing age are consistent with some other reports on warm-blooded horses. The age-related variations in RBC and PCV were less marked. Contrary to some reports, RBC (P < 0.01), WBC and haemoglobin concentration (P < 0.001) were higher in Lipizzan stallions than in mares, but differences in MCHC, MCH and MCV were insignificant. The specific haematological values determined in Lipizzans are presumably a result of selection and should be taken into consideration when dealing with this race of horses. 相似文献