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为探讨内转录间隔区2(ITS2)序列及其二级结构在15种淫羊藿属(Epimedium)中的鉴定效率。利用试剂盒提取叶片总DNA,对ITS2序列进行PCR扩展与测序,计算K2P遗传距离,构建系统发育树,预测其二级结构。结果表明,ITS2序列在15种淫羊藿属植物中的通用性好,扩增成功率与测序成功率均为100%。从K2P种间和种内遗传距离看,ITS2在淫羊藿属中的种间变异较小,部分种类种内遗传距离大于种间遗传距离,鉴定成功率约为60%。采用比对法(Blast)分析,ITS2鉴定效率为75%。由ITS2系统进化树可知,淫羊藿属与鬼臼属各为一支,易于分开。除薄叶淫羊藿、竹山淫羊藿、巫山淫羊藿外,其余种类均能较好的进行聚类;除粗毛淫羊藿、保靖淫羊藿外其余均不能与NCBI下载到的序列进行较好聚类。研究表明,15种淫羊藿属植物ITS2序列在ML系统进化树中具较好的鉴别能力,但淫羊藿属各样本种内与种间遗传较为混乱,遗传距离复杂,ITS2二级结构形态结构亦较为相似,将各种间进行区分存在一定难度,不适合单独作为该属条形码使用。 相似文献
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对原产贵州、四川的15个种、38个居群淫羊藿属植物进行种内杂交、自交结实率统计分析研究.结果显示,该属所有的种类自交结实率普遍较低,最低为0%,最高为22.2%,与种内杂交结实率存在着明显的差异.说明大多数淫羊藿种内自交不亲和,是异花授粉植物,有性繁殖方式可能以风媒授粉为主. 相似文献
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报道贵州省小檗科淫羊藿属特有植物(或已知仅贵州分布植物)10种1变种,分别为黔北淫羊藿(E.borealiguizhouenseS.Z.He & Y.K.Yang)、短茎淫羊藿(E.brachyrrhizum Steam)、德务淫羊藿(E dewuense S.Z.He,Probst & W.F.xu)、普定淫羊藿(E.pudingense S.Z.He,Y.Y.Wang & B.L.Guo)、多花淫羊藿(E.multiflorum T.S.Ying)、剑河淫羊藿(E.myrianthum Steam var.jianheense S.Z.He & B.L.Guo)、小叶淫羊藿(E.parvifolium S.Z.He & T.L.Zhang)、拟巫山淫羊藿(E.pseudowushanense B.L.Guo)、贵州淫羊藿[E.sagittatum (Siebold & Zuccarini)Maximowicz var.guizhouense S.Z.He & B.L.Guo]、水城淫羊藿(E.shuichengense S.Z.He)和单叶淫羊藿(E.simplicifolium T.S.Ying);易危植物2种,分别为小叶淫羊藿和单叶淫羊藿,被《中国物种红色名录》收录.描述了贵州淫羊藿属特有植物和珍稀植物的药用和保护价值及其分布产地.对淫羊藿属存在的问题进行总结分析,并提供贵州省小檗科淫羊藿属植物新名录和以贵州标本命名的淫羊藿属新物种名录. 相似文献
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对原产贵州、四川的14个种、38个居群淫羊藿属植物进行种间杂交结实率统计分析研究。结果显示,淫羊藿属植物种间杂交多数组合有较好的可杂交性,但是不同组合之间有较大的变异。E.wushanense、E.lishihchemii是广亲和的花粉受体,种间杂交中适合作母本。E.myrianthum是广亲和的花粉供体,种间杂交中适合作父本。E.acuminatum、E.pubescense是广亲和的花粉供体兼受体,种间杂交中作为母本或父本都有很高的可杂交性。可确定的生物学物种:大花种类E.pauciflorum、E.acumi-natum、E.chlorandrum,小花类群种类E.sagittatum、E.myrian-thum、E.ecalcaratum、E.pubescense。该结果对于该属进一步的杂交研究、品种改良及该属系统关系研究等都具有一定的参考价值。 相似文献
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通过资源调查,分类学研究,发现贵州有淫羊藿种质资源18种.其中,巫山淫羊藿(E.wushanense)、箭叶淫羊藿(E.sagittatum)为国家药典种;粗毛淫羊藿(E.acuminutum)、天平山淫羊藿(E.myrianthum)、毡毛淫羊藿(E.coactum)、黔岭淫羊藿(E.leptorrhizum)为贵州省地方标准种;黔北淫羊藿(E.borealiguizhouense)、短茎淫羊藿(E.brachyrrhizum)、毡毛淫羊藿(E.coactum)、德务淫羊藿(E.dewuense)、小叶淫羊藿(E.elachyphyllum)、水城淫羊藿(E.shuichengense)、单叶淫羊藿(E.simplicifolium)、普定淫羊藿(E.pudingense)、贵州淫羊藿(Epimedium sagittatum var.guizhouense)为贵州特有种质资源. 相似文献
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本研究通过Illumina HiSeq X-Ten高通量测序平台对粗毛淫羊藿的叶绿体基因组进行测序,并应用生物信息学分析工具对其结构特征进行比较分析。结果表明,粗毛淫羊藿叶绿体基因组大小为156 894 bp,具有被子植物典型的环状四分体结构,大单拷贝区(large single copy, LSC)长度为88 496 bp,小单拷贝区(small single copy, SSC)长度为16 685 bp, 2个反向重复区(inverted repeat sequence, IRa/IRb)长度为25 809 bp;粗毛淫羊藿叶绿体基因组有133个基因,其中包括85个蛋白编码基因、10个rRNA基因和38个tRNA基因,44个外显子,23个内含子。研究结果丰富了淫羊藿属物种的叶绿体基因组资料,可为淫羊藿属物种的分子标记开发、系统学研究与药材的混伪品鉴定提供参考。 相似文献
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介绍了运用冷冻干燥技术进行速溶蛋花汤的加工,研究了生产工艺、特点以及影响产品质量的主要因素。试验表明,采用冷冻干燥技术加工的速溶蛋花汤,具有营养丰富、风味独特、方便快捷等特点。 相似文献
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DENG Zhao-jing 《保鲜与加工》2005,(1):153-156
A new definition of inertia,i.e. the momentum is the measuring of inertia of matter, is proposed. The article through and etc., subjects, the correctness of the new notion of inertia:The monentum is the measuring of inertia has been demonstrated, and the unsolved contradictions of the traditional definition of inetia. And the mass is the measuring of inertia has been revealed. 相似文献
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香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源。但近年来,香蕉生产受到严重的病虫危害。大多数香蕉栽培品种是三倍体,生长周期长,而且不孕。由于没有种子,给繁殖和育种带来一定的困难。遗传转化技术的发展为香蕉品种的改良提供了一种有效的手段。香蕉的遗传转化方法有电激法、基因枪法、农杆菌介导法等。农杆菌介导法的应用是香蕉品种改良的一个重大突破。香蕉遗传转化的外植体也发展到多种,有原生质体,胚性细胞悬浮系,分生组织,以及横切薄片等。近几年,随着分子生物学的发展,出现了转化效率更高,重复性更好的香蕉遗传转化技术。如农杆菌和基因枪结合法,离心辅助农杆菌介导法、真空渗透技术等。这些新技术新方法的出现,必将推动香蕉产业高速发展。 相似文献
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基于RS与GIS技术,利用景观生态学理论及景观格局指数对布尔津县土地利用现状进行定量分析。结果显示为:(1)布尔津县以草地、林地为主要景观类型,镶嵌着耕地、水域等景观类型。(2)四个分区(即高山区、丘陵区、平原区、荒漠区)的林地资源组成差异明显,耕地、居民、交通及工矿用地集中分布在丘陵区和平原区(3)从各项指数来看,高山区的多样性指数较低;平原区是受人文因素最多的区域,景观优势度低,各种土地利用类型比例差异不大,有利于生态可持续发展,但要合理的开发利用;荒漠区生态环境相对脆弱,要加强生态环境的保护与改善。通过该研究对布尔津县的土地利用现状作一个了解,为该县乃至整个地区的景观规划和可持续发展战略提供科学依据。 相似文献
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Efficiency of water use of early plantings of sunflower 总被引:4,自引:0,他引:4
M. Auxiliadora Soriano Francisco Orgaz Francisco J. Villalobos Elías Fereres 《European Journal of Agronomy》2004,21(4):465-476
Rain fed crop production in Mediterranean environments depends to a large extent on strategies that avoid the intense summer drought. Early plantings of sunflower have given consistently higher yields in such environments, but the basis for such yield increases has not been explored. We conducted two field experiments at Cordoba (Spain) to investigate the effects of an early and a late planting date on the components of water-limited crop productivity; namely, water use (T), water use efficiency (TE) and harvest index (HI) of sunflower. The results were generalized by simulating rain fed sunflower yields, under early (1 January) and late (15 March) plantings, for a 25-year period with the aid of a simulation model of the Ceres type (OILCROP-SUN) which has been validated in Cordoba. Experimental seed yields of early plantings in 1989 and 1996 were 2.0 and 3.0 t ha−1, while late plantings yields were 1.3 and 2.4 t ha−1, for the 2 years. Average simulated yields were 2.7 ± 1.1 and 1.9 ± 0.7 t ha−1 for early and late plantings, respectively. For the 2 years, T of early plantings was higher than that of late plantings, but the response of TE and HI to planting date was not the same in the two experiments. In the simulation exercise, T and TE of early plantings were consistently higher than those of late plantings, while there were no differences in the HI for the two planting dates. We conclude that early plantings of sunflower increase rain fed yields by increasing both T and TE, while the impact of planting date on HI very much depends on the crop water stress pattern, which is quite variable from year to year even in the predictable Mediterranean environment. 相似文献
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The cotyledon and hypocotyl of eight varieties of alfalfa( Medicago sativa L. )are used to study the effect of different cultivation procedures and genotypes on embryogenic callus and embryoid induction,plant development and transplant of alfalfa.The varieties of alfalfa with high embryoid induction rate and high differentiation rate are screened. A high frequency regeneration system for alfalfa tissue culture is established:which is benifical to the gene transformation of alfalfa. 相似文献
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Automobile comfort dynamic simulation is necessary when we predict and evaluate the comfort of a automobile or optimize performance of automobile. The nine DOF vibration model of automobile is established , which acts root mean square value of body in multiple work conditions as comfort evaluation indices. Based on the comfort simulation, the Hongyan 1160 is predicted in the way of comfort. The programmes can be used in many different automobiles . The computational result indicates that the model can simulate vibration of automobile truly. It is significant to research automobile comfort evaluation to optimize performance of automobile and comfort simulation .The computational program can be applied to optimize or simulate which has some reference value. 相似文献