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《Scandinavian Journal of Forest Research》2012,27(7):545-556
ABSTRACTSelectively neutral nuclear microsatellites (SSRs) are available for Fagus sp., and have been used in F. sylvatica, an ecologically and economically most important European Fagus species. We provide a comprehensive technical overview of available Fagus sp. SSRs (185 loci), carry out meta-analysis of loci used in F. sylvatica (62 loci employed in 62 surveys), and validate a set of 16 loci in 45 individuals of this species from the central Balkans (Serbia). Erroneous usage of marker’s names/authors is rather frequent, and loci successfully used in a number of studies are characterized by other authors by high prevalence of null alleles and even multilocus amplification products. Frequent occurrence of null alleles at FS4-46, used in 26 surveys to date, most likely indicates a failure to record multiple alleles at this locus. Twelve loci are reliable/informative in F. sylvatica from the Balkans (5–18 alleles/locus, HE ranging from 0.523 to 0.850), while four loci are characterized by high prevalence of null alleles (sfc0161 and sfc1063) and multilocus amplification products (FS4-46 and Fagsyl_007038). Our findings are important for future population genetics and studies on adaptation of F. sylvatica to its environment, because the latter rely on both selectively neutral and adaptive markers under selection. 相似文献
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Bruno Vila Michel Vennetier Christian Ripert Olivier Chandioux Eryuan Liang Frédéric Guibal Franck Torre 《Annals of Forest Science》2008,65(7):709-709
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Liu Di Zhang Xiao-mei Hua Xin Qiao Nan Song Han Lu Hai Guo Hui-hong Li Feng-lan 《中国林学(英文版)》2007,9(2):127-131
Telomeres are the structures that locate at the terminals of linear eukaryotic chromosomes. They can play essential roles in many cellular processes. The terminal location of Arabidopsis-type TTTAGGG tandem repeats were thought to be highly conserved. The terminal location of Ginkgo biloba L. consisting of TTTAGGG tandem repeats, were confirmed by Bal31 exonuclease degradation and Southern blotting. By comparing telomeric restriction fragment (TRF) lengths at different developmental stages from embryos to seedlings, a fluctuant tendency towards variation was found in these samples. The TRF length of embryos was also compared with that of embryonal calli and an upward trend was discovered in callus culture. The results suggest that there should be a telomerase mechanism or/and ALT mechanism for the maintenance of telomere length. 相似文献
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A reliable,efficient anther culture system,the dominant technique for generating haploid plants in breeding programs,that can be used for generating transgenic poplar plants has been needed.In the present study,therefore,an anther culture system was developed using isolated mid-and late-uninucleate anthers of poplar(Populus simonii x P.nigra).From a combination of SSR and ploidy analyses,six double haploid and two haploid lines were characterized from 86 plants grown from 16 regenerated anther cultured lines.After 48 months of development,two plant lines from the regenerated plants maintained their haploid level in vitro for over 2 years.A number of haploid plants from the different lines weretransferred to soil.The leaves of these transplants were then used as explants for transformation with the APETALA1(AP1) gene using Agrobacterium tumefaciens.Overexpression of AP1 in haploid poplar induced early flowering with obvious petals when ectopically expressed.To our knowledge,this is the first report on changes in flowering time in AP1-trangenic poplar,which is important for elucidating the regulatory mechanism of tree flower development. 相似文献
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