栗山天牛生物学特性及防治技术研究

栗山天牛是柞树的重要蛀干害虫，近年在辽宁省发生面积已达６～７万ｈｍ＾２。受害后的柞树材积生长及材质下降，甚至造成树木枯死。为有效控制栗山天牛危害，１９９５～１９９９年，进行生物学特性及防治技术的研究，结果查清了栗山天牛的生活史，掌握了栗山天牛的生物学特性。防治试验证明，采用卫生伐、人工捕捉、利用天敌及熏蒸剂，都能经济有效地防治栗山天牛。     

Consequences of cutting off distal ends of cotyledons of Quercus robur acorns before sowing

• Background   

Production of seedlings, especially in containers, requires simultaneous germination and emergence. Mechanical scarification often speeds up the growth of embryo axes, increases the percentage of germinating seeds and seedling emergence. Cutting off the distal ends of cotyledons is a mechanical scarification technique sometimes used in the container production of oak seedlings. However the consequences of this procedure for seedling development are little known. We wanted to determine these effects on development and metabolic changes of pedunculate oak (Quercus robur L.) seedlings.     

Soil water content and infiltration in agroforestry buffer strips

Agroforestry practices are receiving increased attention in temperate zones due to their environmental and economic benefits. To test the hypothesis that agroforestry buffers reduce runoff by increased infiltration, water use, and water storage; profile water content and soil water infiltration were measured for a Putnam soil (fine, smectitic, mesic Vertic Albaqualf). The watershed was under no-till management with a corn (Zea mays L.)-soybean (Glycine max L.) rotation since 1991. Agroforestry buffer strips, 4.5 m wide and 36.5 m apart, were planted with redtop (Agrostis gigantea Roth), brome (Bromus spp.), and birdsfoot trefoil (Lotus corniculatus L.). Pin oak (Quercus palustris Muenchh.), swamp white oak (Q. bicolor Willd.) and bur oak (Q. macrocarpa Michx.) trees were planted at 3-m intervals in the center of the agroforestry buffers in 1997. Ponded water infiltration was measured in agroforestry and grass buffers and row crop areas. Water content in agroforestry and row crop areas at 5, 10, 20, and 40 cm depths were measured throughout the year. Quasi-steady infiltration rates were not different (P > 0.05) among the treatments. Agroforestry had lower soil water content than row crop areas (P < 0.05) during the growing season. Higher water content after the principal recharge event in the agroforestry treatment was attributed to better infiltration through the root system. Results show that agroforestry buffer strips reduce soil water content during critical times such as fallow periods, and increase water infiltration and water storage. Therefore, adoption of agroforestry buffer practices may reduce runoff and soil loss from watersheds in row crop management.     

Efficacy of local eradication treatments against the sudden oak death epidemic in Oregon tanoak forests

Phytophthora ramorum, cause of sudden oak death, has been distributed widely across the United States in horticultural situations, but is not established in forests outside of California and Oregon. Here, it has triggered widespread concern and, especially in Oregon, an intensive disease management programme. Now, we provide the first systematic evaluation of the efficacy of that effort. This paper evaluates four measures of the efficacy of Sudden Oak Death (SOD) local eradication treatments: inoculum availability; inoculum from tree species other than tanoak; disease spread from treated areas; and cumulative infested area with and without treatment. We conclude that local treatments demonstrably reduce local inoculum levels. Eradication of SOD from infested sites is difficult but not impossible. The disease usually does not persist after cutting infected trees but spread on the landscape continues because the pathogen may be present on undetected new infections for a year or two before whole tree symptoms are visible. This limits early detection and coupled with delays in completing eradication treatments, prolongs the chances for long‐distance aerial dispersal.     

Influence of soil properties and burial depth on Persian oak (<Emphasis Type="Italic">Quercus brantii</Emphasis> Lindl.) establishment in different microhabitats resulting from traditional forest practices

In seasonally dry environments such as the Zagros woodlands (Iran), severe drought stress and lack of appropriate management practices can cause failure of oak afforestation or reforestation. We investigated the effect of soil properties and burial depth on Persian oak (Quercus brantii Lindl.) establishment in different microhabitats resulting from traditional forest practices. Four microhabitats that were based on forest structure were considered for oak acorn seeding: (1) inside old sprout clumps (ISPC); (2) under the canopy of tall trees (UCTT); (3) outside the canopy of tall trees and sprout clumps; and (4) near recent stumps or sprout clumps. Acorns were seeded at two depths (2 and 5 cm), and seedling survival and growth variables were recorded for 4 years, together with soil chemical and biological attributes. Stepwise discriminant analysis showed that a combination of total soil nitrogen, cation exchange capacity, available phosphorus and potassium, litter depth, microbial quotient, metabolic coefficient, substrate-induced respiration and earthworm abundance was the best variables to characterise the microhabitats. With the exception of pH, bulk density and soil texture, these variables were higher in UCTT and ISPC than in the other microhabitats. Seedling emergence and survival were greater at a seed depth of 5 cm than at 2 cm. Seedling height and shoot, root and leaf biomasses were higher in the UCTT microhabitat compared to the other microhabitats and correlated positively with soil nutrients contents and most of the soil biological variables but negatively with soil bulk density. This study emphasised the role of microhabitats in creating a “canopy effect” producing favourable physical, chemical and biological soil conditions. In particular, large oak trees form islands of fertility and therefore are of key importance for successful seedling establishment in forests subjected to intense human activities.     

Effects of roe deer browsing and site preparation on performance of planted broadleaved and conifer seedlings when using temporary fences

Abstract

The effects of fencing and site preparation on performance of birch (Betula pendula Roth), oak (Quercus robur L.), pine (Pinus sylvestris L.) and spruce [Picea abies (L.) Karst.] seedlings were studied over four growing seasons in southern Sweden. The experiment was composed of four browsing treatments, from which roe deer (Capreolus capreolus L.) were excluded for 0, 12, 24 or 42 months, and four soil treatments. Natural browsing was combined with artificial browsing (clipping). It was demonstrated that browsing by roe deer could be isolated from browsing by moose (Alces alces L.) by selective fencing. Except for birch, browsing had little effect on seedling survival. Browsing reduced height growth by more than 100% for oak and pine, and more than 60% of pine seedling developed multiple stems. Except for oak, site preparation increased seedling survival. Inverting site preparation, in combination with or without fertilization, produced positive growth responses in seedlings, whereas patch scarification did not. There was no interaction between browsing and soil treatments on seedling performance. It was concluded that long-term protection against browsing by roe deer is needed for oak and pine, whereas short-term fences might be used for birch, and that spruce is relatively unaffected by browsing.     

Relationship between field resistance to Phytophthora ramorum and constitutive phenolic chemistry of coast live oak

Sudden oak death, caused by Phytophthora ramorum, has resulted in high levels of coast live oak (CLO) mortality. However, some CLO survive in areas with high disease pressure and may thus be resistant. We tested the hypothesis that such field‐resistant trees contain constitutively higher levels of phenolics than susceptible trees. Phloem was sampled from the trunks of two groups of trees (one previously inoculated, one naturally infected with P. ramorum) categorized over the course of several years as putatively resistant (PR, no symptoms), in remission (IR, showed symptoms but then recovered) and symptomatic (S). Individual and total soluble phenolics from these trees were quantified. There were no significant differences in individual or total soluble phenolics between groups of naturally infected trees. However, inoculated PR and IR trees were characterized by higher constitutive levels of ellagic acid, a tyrosol derivative, and an unidentified phenolic than S trees. Ellagic acid and tyrosol‐like compounds in CLO phloem are promising resistance biomarker candidates.     

Spatial patterns and environmental factors affecting the presence of Melampsorella caryophyllacearum infections in an Abies alba forest in NE Spain

The presence of trunk swellings caused by the rust fungus Melampsorella caryophyllacearum was systematically surveyed in an Abies alba forest (Irati, NE Spain), using 1237 circular plots (diameter = 18 m). The relationship between fungal presence and several abiotic (aspect, elevation, distance to the nearest river and slope) and biotic factors (basal area of A. alba and/or Fagus sylvatica, shrub, fern and herb cover) was assessed through correlation and ordination analyses. Additionally, the spatial pattern of the presence of diseased trees was described using Ripley's K function. Southern‐aspect plots had a significantly lower presence of diseased trees than plots‐oriented north, east and west. Plots with diseased trees were located at a significantly lower elevation, and at a shorter distance to the river than plots without infections. Plots with diseased trees had almost twice the average A. alba basal area, and less average F. sylvatica basal area than plots without diseased trees. However, similar mean values of slope and shrub, fern and herb cover were found in both types of plots. The disease showed spatial aggregation in patches with a mean radius of ca. 900 m. The implications of the results for disease management are discussed.     

Thinning around old oaks in spruce production forests: current practices show no positive effect on oak growth rates and need fine tuning

The expansion of spruce-dominated forestry in Southern Sweden during the twentieth century has led to a considerable amount of oak (Quercus robur L.) woodlands being converted into stands dominated by planted spruce. The thinning of spruces around oak trees is currently done in Sweden to improve local diversity of insects, oak growing conditions and eventually decrease their mortality. To evaluate the effect of these treatments, we dendrochronologically studied growth of old (100–200 years old) oaks subjected to thinning of different intensity at nine locations in southern Sweden, and compared them to oaks located in nearby pastures. The overall pattern suggests that commonly adopted thinning intensities do not significantly affect oak growth. Oak growth was positively related to oak age and negatively to the amount of dead oak crown. Analyses of correlations between oak growth and summer drought conditions, as reflected by location-specific chronologies of the Monthly Drought Code (MDC), indicated that older trees exhibited generally negative correlations, whereas the correlation remained generally positive for the younger trees, both inside and outside forest stands. We propose that removal of spruces should be primarily done around older and healthier-looking trees.     

Treatment of oak decline using pressurized injection capsules of antifungal materials

Quercus ilex and Quercus suber trees growing at several sites in Extremadura, Western Spain, that were showing symptoms of oak decline were injected with potassium phosphonate, quinosol or carbendazim using a low-pressure method of trunk injection composed of a pressurized capsule system. A team of four people injected between 120 and 189 trees per day, depending upon the density of the undergrowth vegetation. This labour cost represented, approximately, 15–20% of the total cost of the treatment. The potassium phosphonate-injected trees showed a significant improvement in vegetative growth within 2 years of the injection treatment, and they also showed some recovery from the decline symptoms during the third year. Only one injection treatment of an average of 3.5 capsules (corresponding to 24.5 g phosphonic acid) per tree of approximately 36 cm in diameter, was necessary to reduce the disease severity significantly. Indirectly, these results corroborate the implication of Phytophthora cinnamomi in oak decline within Spanish Quercus woodlands.     

Variation of endophytic cork oak‐associated fungal communities in relation to plant health and water stress

The main objective of this study was to obtain more comprehensive knowledge about the effect of water stress on endophytic fungal communities in asymptomatic and declining cork oak trees. Six asymptomatic and six declining cork oak trees were randomly selected in a natural cork oak forest located in Sardinia, Italy. In February 2003, the soil around three asymptomatic and three declining trees was covered with a circular plastic film to reduce rain water supply with the intention to induce water stress. The remaining six trees served as controls. Predawn xylematic water potential (PWP) was used as water status indicator and measured seasonally. Between July 2003 and June 2004, fungal endophytes were isolated every 2–4 months from twigs, branches and woody tissues. Significant differences in PWP between covered and control trees were detected mainly in autumn. Gas exchange was greatest in asymptomatic control plants. All tissues were colonized by endophytic fungi. Nineteen fungal species were isolated from 1620 plant fragments. Biscogniauxia mediterranea was the most frequently isolated fungus. Its isolation frequency was significantly higher in declining covered trees than in control trees (p < 0.05). Presence of this fungus in asymptomatic control trees was significantly higher in winter than in summer. Water stress seems to reduce species diversity of the endophytic mycobiota in cork oak and to promote proliferation of some potentially pathogenic endophytes.     

Effects of Mechanized Thinning and Prescription Fire on Stand Structure,Live Crown,and Mortality in Jeffrey Pine

ABSTRACT

Forest thinning, using cut-to-length and whole-tree harvesting systems with subsequent underburning were assessed for their influence on stand structure, health, and fire resilience in uneven-aged Jeffrey pine (Pinus jeffreyi Grev. & Balf). Stand attributes, derived from measuring trees ≥ 10.2 cm diameter at breast height (DBH), were collected from permanent plots. Trees were divided into three size classes that generally corresponded to dominant/codominant, intermediate, and suppressed crown classes. Comparisons of post- to pre-burning mortality revealed significant thinning and fire main treatment effects as well as significant interaction between these two treatments in the two larger size classes. Mortality increased by 250% in the intermediate crown class within the burned stand portion of the whole-tree treatment, whereas among dominant/codominant trees mortality rose by 160% in the burned cut-to-length treatment combination. Pre- to post-burning shifts in live crown, expressed as a percentage of total tree height, were significantly influenced by both thinning and fire main treatments in the two larger size classes, while the interaction of these treatments was also significant among the largest trees. Within both of these size classes, decreases in live crown percentage were greatest in the burned portion of the unthinned treatment, where intermediate crown class trees lost over 20% of their crowns, while reductions in dominant/codominant trees averaged nearly 25%. The second highest losses for both size classes occurred within the burned cut-to-length treatment. In the smallest trees, mortality rose sharply and live crown decreased substantially after burning in both thinning treatments and in the unthinned control. Within the two larger size classes, preburn live crown size was negatively correlated with changes in crown size subsequent to underburning while DBH was negatively correlated with postburning changes in mortality, but only in intermediate crown class trees. These results present land managers with outcomes of differing management practices presently being evaluated for their potential to enhance forest health and reduce wildfire risk in the Sierra Nevada and similar dry forest regions.     

Survival of Ceratocystis fagacearum in branches of trees killed by oak wilt in Minnesota

The survival of Ceratocystis fagacearum in the branches of Quercus ellipsoidalis killed by oak wilt in Minnesota depends on the time of year when the trees die. In trees, which wilt in May or June, the pathogen can be isolated from the xylem for only 1–2 months. In trees which die later in the summer it may persist through to the following spring. Observations on oak bark beetles (Pseudopityophthorus spp.) breeding in the dead trees do not suggest that these beetles are important vectors of the disease in Minnesota.     

Pedunculate oak (Quercus robur L.) silviculture in natural stands of NW Spain: Environmental conditioners

Forest management is often carried out in different ways, without any appropriate environmental restrictions. Stands of pedunculate oak (Quercus robur L.) in Galicia (NW Spain) have been harvested by alternating high forest and, mainly, coppice forest. However, some totally inappropriate silvicultural treatments have been used, such as thinning of the best trees and inadequate pruning. The objective of the present study was to analyse how environmental characteristics affect the management of oak forests in Galicia. For this, a botanical inventory was carried out in 39 selected stands of Q. robur and a total of 42 parameters were measured, 4 of which were physiographical, 12 climatic, 19 edaphic and 7 silvicultural. In order to analyse the possible relationships among these variables, the silvicultural data were compared with the other data, by canonical correlation analysis. All parameters were correlated with the silvicultural regime, although the correlation was weak for the floristic data. It is therefore evident that the environmental conditions affect how forest stands should be managed, although this does not imply that more profitable use of the stands cannot be achieved than at present, and alternative silvicultural methods must be found to enable appropriate management and conservation of oak stands.     

La variabilité de la sensibilité du Hêtre (Fagus sylvatica L.) à Nectria coccinea,un des agents de la maladie de l'écorce

Variability of Fagus sylvatica susceptibility to Nectria coccinca, one of the agents of beech bark disease . N. coccinea was inoculated into wounds of young beeches raised from seeds of 6 bark diseased trees and 6 healthy ones in the same stand of the forest of Lyons. Classifieation of the progenies, according to the size of the necrosis, is in good agreement with the health of the mother trees. N. coccinca susceptibility of beech has a genetic origin.     

Brenneria spp. and Rahnella victoriana associated with acute oak decline symptoms on oak and hornbeam in Iran

Decline diseases of forest trees are complex syndromes not attributable to single causal factors. In Iran, symptoms of decline disease have been observed in a number of native forest species including Quercus castaneifolia (chestnut‐leaved oak), Q. brantii (Persian oak) and Carpinus betulus (hornbeam). The symptoms are prevalent in the northern forests and the Zagros mountain forests. There are parallels between the disease in Iran and acute oak decline (AOD) reported in the UK, specifically the presence of weeping cankers, which have been attributed to a polybacterial complex wherein Brenneria goodwinii is considered a key necrogen. Based on the AOD symptomatology, and as a first step towards discovering potential causal agents of the stem weeping symptoms of affected trees in Iran, necrotic tissues were tested primarily for the presence of B. goodwinii. Symptomatic Q. castaneifolia and C. betulus from the Mazandaran Province and symptomatic Q. brantii from Kohgiluyeh and Boyerahmad Province were sampled. Isolation and culture on a selective medium yielded uniform bacterial colonies. Isolates were characterized using phenotypic and genotypic (DNA sequencing) tests. The isolates were phenotypically identical to members of Pectobacteriaceae and Yersiniaceae, specifically Brenneria and Rahnella spp. The nucleotide sequences of the 16S rRNA and housekeeping genes gyrB, infB and atpD (MLSA) amplified via PCR demonstrated that the isolates from the trees in Iran were indeed B. goodwinii, B. roseae subsp. roseae, Rahnella victoriana and an unknown species of Brenneria. Most bacteria isolated from non‐symptomatic trees were Gram‐positive, and Pseudomonas spp. were dominant, but bacterial species isolated from the diseased trees were not detected in healthy trees. Hypersensitivity response tests were positive, but inoculation on saplings was more variable with internal necrosis developing only once in the test period. Therefore, further testing is required. This is the first report of the incidence of B. goodwinii, B. roseae subsp. roseae, R. victoriana and Brenneria sp. associated with acute oak decline‐like symptoms on Q. castaneifolia, Q. brantii and C. betulus across the western forests of Iran and in the world.     

Effects of silvicultural disturbance on acorn infestation and removal

Oak (Quercus spp.) regeneration is an important forest management goal in the central hardwood forest region of the United States. Silvicultural methods that target oak regeneration, such as the creation of canopy openings and removal of shade-tolerant midstory trees, are complicated by the impact of pre- and post-dispersal predators of oak acorns like acorn weevils (Curculio spp.) and granivorous small mammals. Understanding the effects of forest disturbance created by silviculture on these predators is important to promote successful oak regeneration. We conducted a 6-year study of acorn production, weevil infestation, and acorn removal at 113 black (Q. velutina) and white oak (Q. alba) trees impacted by three types of silvicultural treatments: adjacent to harvest openings, midstory removal (first-stage shelterwood harvest), and control (no harvest) in south-central Indiana, USA. We observed highly variable acorn production across years, but minimal impact of silvicultural treatments. Levels of weevil infestation and acorn removal by small mammal seed predators also varied greatly, and predation pressure was highest in years when acorns were scarce. Weevil infestation was reduced following midstory removal in shelterwood harvests, but probability of acorn removal by small mammals was unchanged following harvest. Damaged, germinated, and weevil-infested acorns were less likely to be removed by seed predators, suggesting additive effects of pre- and post-dispersal predators. This study emphasizes the importance of considering acorn predators in oak regeneration silviculture, and timing harvests to follow large acorn crops in order to reduce predation pressure and generate a high number of seedlings.     

The larger,the better? Effects of delayed diameter-limit cutting on old-growth attributes and saproxylic beetle diversity in temperate oak forests

Intensive management implies harvesting large, old trees, which reduces the old-growth attributes. This negatively affects biodiversity, especially saproxylic beetles. In managed temperate oak forests, rotation extension induced by increasing the diameter threshold of final harvest by about 10 cm compared to conventional practices (i.e. DBH around 70 cm) might mitigate this negative effect. Here, we used a gradient of the proportion of overmature trees (DBH?≥?80 cm) among mature trees (DBH?≥?70 cm) across plots of high oak French forests to test the potential effect of increasing diameter threshold on (1) structural features and (2) species composition and diversity of saproxylic beetles communities. We assessed deadwood and microhabitats availability (i.e. volume/density and diversity) and canopy openness in 81 1-ha plots across eleven French forests. Results highlighted that a larger proportion of overmature trees, for a given density of mature trees, had limited effects on structural features: only cavities density showed a significant positive response, with no cascading effect on cavicolous beetles. Moreover, the proportion of overmature trees had no significant effect on the composition of saproxylic beetles communities (and ecological variables altogether explained only 17% of the composition inertia). By contrast, mature tree density enhanced microhabitat density and indirectly increased the abundance of rare species. Thus, shifting DBH from 70 to 80 cm in high oak forest could have no or limited effect on saproxylic beetles conservation. Improved strategies might rather stem from combining (1) longer rotation extension and (2) less intensive management practices in extended rotation stands.     

Bacillus pumilus and Stenotrophomonas maltophilia as two potentially causative agents involved in Persian oak decline in Zagros forests (Iran)

The oak decline is known as one of the most destructive complex diseases causing high economic losses around the world, especially in Iran. The main objective of the present study was to investigate the possible role of bacteria as causative agents of oak decline in the Zagros forests of Iran. To do this, stem, root and leaf samples were taken from symptomatic Persian oak trees (Quercus brantii) in different zones of Zagros forests (Ilam Province, Iran). From 150 bacterial isolates, 20 showed pathogenicity against Geranium seedlings. Among 20 hypersensitivity test positive strains, four strains showed pathogenicity against oak saplings. Based on morphological and 16S rRNA gene sequence analysis, three strains were identified as Bacillus pumilus and one strain as non‐sporulating Gram‐negative Stenotrophomonas maltophilia. Pathogenicity studies of different B. pumilus and S. maltophilia strains revealed that they have potential to cause the disease in oak saplings and symptoms of disorder in Persian oak trees. To our knowledge, there are no previous records of B. pumilus and S. maltophilia causing decline on Fagaceous trees like Q. brantii. More detailed field and molecular studies are required to confirm the absolute role of such bacteria in occurrence of oak decline in Zagros forests.     

Browsing damage on broadleaved trees in semi-natural temperate forest in Sweden,with a focus on oak regeneration

Absence of, or poor, oak (Quercus spp.) regeneration is a problem in uneven-aged, mixed closed-canopy broadleaved forests. Browsing by ungulates on small trees may contribute to poor oak regeneration in such forests. This possibility was investigated in 25 Swedish stands, and browsing damage was analysed in relation to landscape and stand factors. The proportion of browsed small (<20 cm tall) oak seedlings and other seedlings was low, and apparently a minor mortality factor. For saplings (20–130 cm tall), accumulated browsing damage was generally higher on oak than on five major competing tree species: Fraxinus excelsior, Corylus avellana, Tilia cordata, Acer platanoides and Sorbus aucuparia. Leaf removal was rare in late summer, except for rowan. The amount of cover (shelter) for ungulates near plots was positively correlated with oak browsing intensity; within plots, a high density of ash saplings may reduce browsing on oak saplings. In these forests, browsing probably retards growth of oak saplings relative to competing trees. Oak may persist as a minor stand component, but monitoring is needed to study future changes.