Pharmacokinetics and tissue residues of marbofloxacin in crucian carp (Carassius auratus) after oral administration

Pharmacokinetics and residue elimination of marbofloxacin (MBF) were studied in crucian carp (Carassius auratus, 250±30 g) kept at two water temperatures of 15 and 25 °C. Marbofloxacin concentrations in plasma and tissues were analysed by means of high‐performance liquid chromatography using an ultraviolet detector. The limits of detection were 0.02 μg mL^?1, 0.02 μg g^?1, 0.025 μg g^?1, 0.02 μg g^?1 and 0.025 μg g^?1 in plasma and muscle, skin, liver and kidney respectively. Fish were administered orally at a single dosage of 10 mg kg^?1 body weight in the PK group. The data were fitted to two‐compartment open models at both temperatures. At 15 °C, the absorption half‐life () and distribution half‐life (t_1/2α) of the drug were 0.36 and 4.48 h respectively. The corresponding values at 25 °C were 0.23 and 0.87 h respectively. The elimination half‐life (t_1/2β) was 50.75 h at 15 °C and 25.05 h at 25 °C. The maximum MBF concentration (C_max) differed little between 15 (6.43 μg mL^?1) and 25 °C (8.36 μg mL^?1). The time to peak concentration was 1.74 h at 15 °C and 0.78 h at 25 °C. The apparent volume of distribution (V_d/F) of MBF was estimated to be 1.36 and 0.87 L kg^?1 at 15 and 25 °C respectively. The area under the concentration–time curve (AUC) was 301.80 μg mL^?1 h at 15 °C and 182.80 μg mL^?1 h at 25 °C. The total clearance of MBF was computed as 0.03 and 0.05 L h^?1 kg^?1 at 15 and 25 °C respectively. After repeated oral administration at a dosage of 10 mg kg^?1 body weight per day for 3 days, the results showed that the elimination half‐lives () of MBF from all tissues at 15 °C were longer than that at 25 °C. Therefore, water temperature is an important factor to be considered when deciding a reasonable withdrawal time.     

Pharmacokinetics of minocycline in crucian carp (Carassius auratus) after intravenous and oral administration

The pharmacokinetic of minocycline was studied after a single intravenous as well as oral dose (5 mg/kg body weight) in crucian carp (Carassius auratus) reared in freshwater at 10°C. Plasma samples were randomly collected from six fish at each sampling time. Plasma concentrations were determined by high‐performance liquid chromatography and further subjected to noncompartmental analysis. Initial concentration of minocycline just after intravenous administration was calculated as 7.320 μg/ml, while the other parameters after intravenous injection were determined as flows: apparent elimination rate constant (λ_z) of 0.064 per hr, apparent elimination half‐life () of 10.82 hr, total body clearance (Cl) of 142.72 ml/hr/kg, volume of distribution (Vz) of 2,227.38 ml/kg and volume of distribution at steady‐state (Vss) of 1,937.08 ml/kg. While after oral administration, the λ_z, , mean absorption time (MAT), absorption half‐life (t_1/2ka) and bioavailability were determined as 0.059 per hr, 11.74, 5.55, 3.84 hr, and 81.98%, respectively, and the peak concentration was observed as 1.474 ± 0.362 μg/ml at 8 hr. It was shown that minocycline was slowly but relatively completely absorbed, extensively distributed, and slowly eliminated in crucian carp. Based on the ratios of AUC_0–24 hr/MIC90, a minocycline dosage of 5 mg/kg body weight administered intravenously or orally would be only effective to successfully treat crucian carp infected by bacterium with MIC values ≤0.25 μg/ml.     

Effect of dietary phosphorus levels on growth and body composition of crucian carp,Carassius auratus under indoor and outdoor experiments

Indoor and outdoor experiments were carried out simultaneously to evaluate the effects of dietary phosphorus levels on growth performance and body composition of crucian carp. Monocalcium was used to formulate extruded feed containing available phosphorus (AP) of 1.5 (D1), 4.7 (D2), 6.3 (D3) and 9.0 (D4) g kg^?1. Twelve 500‐L recirculating tanks were assigned to four triplicate groups with each tank stocked with 15 juveniles (39.88 ± 0.14 g) for indoor experiment. Twelve polyethylene enclosures were assigned to four groups with each one stocked with 120 juveniles (44.08 ± 1.36 g). After 8 weeks of feeding, survival ranged from 96% to 100%. Weight gain, special growth rate, protein efficiency ratio and feed conversion rate did not show significant differences among dietary treatments in both experiments. Fish cultured in the enclosures grew better, although D1 and D2 treatments did not reach significant level. Crude protein and phosphorus content of the fish were not significantly affected by dietary phosphorus level. Moisture, crude lipid, crude ash and vertebrae phosphorus were significantly affected by different phosphorus levels. Based on vertebrae phosphorus, crucian carp cultured in the tanks and in the enclosures showed phosphorus deficiency under 4.7 g kg^?1 and 6.3 g kg^?1 AP, respectively.     

葡萄糖、维生素C对普安银鲫早期发育的影响

为探究葡萄糖和维生素C（VC）对普安银鲫（Carassius auratus gibelio）早期发育的影响,采用静水溶液浸泡法研究了葡萄糖和VC溶液浸泡中普安银鲫仔鱼出膜时间、孵化率、成活率及卵黄囊仔鱼生长特点。结果显示,葡萄糖溶液质量浓度低于15 g·L^-1,仔鱼出膜时间随着葡萄糖质量浓度的增加而缩短,孵化率、成活率、体质量和全长则升高;葡萄糖溶液质量浓度高于15 g·L^-1对普安银鲫的早期发育表现出抑制作用。VC溶液质量浓度低于30mg·L^-1时,仔鱼出膜时间随着ρ（VC）的增加而降低,孵化率、成活率、体质量和全长则升高。ρ（VC）高于30mg·L^-1对普安银鲫的早期发育表现出抑制作用。研究表明适宜浓度的葡萄糖和VC能促进普安银鲫的早期发育。     

饲料Vc对异育银鲫的生理效应及其适宜添加量

在精制饲料中分别添加不同剂量Vc(0、37.5、75、15 0、30 0、6 0 0和 12 0 0mg/kg) ,连续投喂异育银鲫 (方正银鲫♀×兴国红鲤♂ ) (Carassiusauratusgitelio) 8周。结果显示 ,当饲料中缺乏Vc时异育银鲫虽没有出现明显的缺乏症状 ,但日增重率、血红蛋白含量和血清POD酶活性均低于Vc添加组 ,而红细胞脆性极显著地高于高剂量Vc添加组 (P <0 .0 1) ;同时 ,经新复极差检验 ,在适宜范围内随着饲料Vc含量增加 ,异育银鲫日增重率、血红蛋白含量、POD活性等指标显著提高 ,而红细胞脆性明显下降 (P <0 .0 5 )。综合以上指标的非线性回归分析结果 ,建议生产上异育银鲫饲料Vc适宜添加量为 30 0～ 5 0 0mg/kg。     

异源四倍体鲫鲤及其原始亲本遗传变异的RAPD分析

在优化RAPD检测条件的基础上，从100个随机引物中筛选出60个扩增较好的引物对异源四倍体鲫鲤及其原始亲本红鲫和湘江野鲤相互间扩增DNA片段的异同性进行比较研究。结果显示，60个引物共产生1554条带，单一引物扩增的DNA片段数在6～20条之间，片段大小在200～3500bp之间。遗传相似率分析表明，异源四倍体鲫鲤与其原始母本红鲫的相似率为69．41％，与其原始父本野鲤的相似率为64．47％，说明异源四倍体鲫鲤接受原始母本红鲫的遗传物质要多一些；而红鲫和野鲤之间的相似率为42．18％，说明两者的基因组成有较大的相似性，从分子水平证实了红鲫和野鲤的远缘杂交具有较大的亲和性。在分析中，还发现异源四倍体鲫鲤有4条非亲本带，非亲本带谱率达0．72％，这些非亲本带可以作为异源四倍体鲫鲤特异性的分子标记。     

洞庭青鲫等5个鲫品系线粒体ATPase基因序列的比较分析

运用PCR扩增、克隆、测序等技术对洞庭青鲫(Carassius auratus var.Dongtingking)、彭泽鲫(C.auratus var.Pengze)、普通鲫(C.auratus)、红鲫(C.auratus var.red)、日本白鲫(C.auratus cuvieri)等5个鲫品系线粒体DNA ATP...     

急性氨氮胁迫对淇河鲫幼鱼脑、鳃、肝、肾组织结构的影响

随着养殖密度的增加,过量投喂产生的残饵和大量代谢废物在水中积累,并通过氨化作用转化为氨态氮在水体中富集,对鱼类的抗氧化系统、呼吸及神经系统均存在较大的危害。本研究以6月龄淇河鲫（）幼鱼[体重（15±2）g]为实验对象,研究水体中过量氨氮（50 mg/L、100 mg/L、150 mg/L、200 mg/L、250 mg/L、300 mg/L）对淇河鲫脑、鳃、肝、肾的组织学损害。结果发现,淇河鲫安全养殖的氨氮浓度为16.6 mg/L,超过安全浓度后随着氨氮浓度的升高及氨氮胁迫时间的延长,脑神经纤维结构被破坏的程度增大,神经细胞体出现不同程度坏死;鳃小片也出现不同程度的萎缩聚拢,鳃丝基部充血肿大,鳃丝间隔增大,上皮细胞排列紊乱,泌氯细胞和上皮细胞出现不同程度的空泡化,超显微观察发现,鳃小片出现萎缩,上皮细胞脱落,细胞表面分泌物增多;肝组织中心静脉周围肝细胞排列出现不同程度的紊乱,肝小叶轮廓模糊不清且间距增大,肝细胞排列疏松,细胞核出现不同程度的偏移、溶解,细胞表面粗糙且分泌物增多;肾组织出现淋巴细胞浸润和扩散、肾小囊腔膨大、肾小管腔缩小的现象,甚至出现了肾小球坏死。以上结果表明高浓度氨氮对淇河鲫幼鱼的神经、呼吸及代谢相关器官均存在不同程度的损害。因而,在淇河鲫大规模养殖过程中,应严格监测水体中氨氮浓度,践行绿色、健康养殖模式。     

Thermal resistance traits of the clonal silver crucian carp Carassius langsdorfii and evaluation of these traits using primary culture cells

The thermal resistance traits of three clonal lines of the silver crucian carp Carassius langsdorfii (SCC-1, -2 and -3) were investigated. Individual juvenile fish reared at 20°C were exposed to thermal stress at 36.5 ± 0.5°C and their death times during this exposure were measured. The death times of SCC-2 and SCC-3 fish were 66.6 ± 31.2 and 144.7 ± 49.8 min, respectively. In contrast, all SCC-1 fish survived under these conditions. Furthermore, the thermal sensitivity of primary culture cells from each clone was determined at 37, 40 or 43°C by the Trypan blue assay. Under all treatment conditions, the thermal sensitivity of the SCC-1 primary cultures was lower than those of the others. These results estimated the correlation between the in vivo and in vitro thermal resistances. Therefore, the use of primary culture cells to evaluate thermal resistance could be a useful method of selection breeding.     

Effects of a dietary administration of purple coneflower (Echinacea purpurea) on growth,antioxidant activities and 8 miRNAs expressions in crucian carp (Carassius auratus)

Echinacea purpurea (EP), a globally popular herbal medicine, has been used to treat various diseases in human and animals. However, little has been reported about its effects in fish. In this study, crucian carp (Carassius auratus) were selected to evaluate the effects of EP on growth performance and antioxidant response and the expressions of microRNAs. The results showed EP could stimulate the growth of crucian carp with the best effect was observed at dose of 4 g kg^?1. In serum, the content of hydroxyl radical (·OH) and malondialdehyde (MDA) decreased by EP supplementation, whereas the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) increased. Similarly, the content of ·OH decreased, and the activities of CAT, GPX and GR increased in liver of crucian carp. Furthermore, in livers of crucian carp, EP supplements upregulated the expressions of the microRNAs (miR‐16, miR‐21, miR‐125b, miR‐146a, miR‐155, miR‐181a and miR‐223), which had been confirmed to participate in regulating antioxidant and immune function in mammals. Our results suggest EP supplements in diets stimulated growth performance and antioxidant response of crucian carp. In liver, the upregulation of specific miRNAs may participate in the antioxidant effects of EP diets.     

鲫血髓过氧化物酶的表达及其与血药浓度的关联性

为了研究在吡喹酮代谢过程中,鲫血髓过氧化物酶(myeloperoxidase,MPO)m RNA表达与血药浓度的相关性,以体重为(80±10.5)g的鲫鱼(Carassius auratus)为试验动物,单剂量(10 mg/kg)口灌吡喹酮(praziquantel,PZQ)后,在鲫血液中选取β-actin为内参基因,通过设计特异性引物,利用荧光定量PCR,分析了不同时间点鲫血MPO m RNA水平的相对表达量的变化;利用高效液相色谱法(high performance liquid chromatography,HPLC)测定了吡喹酮在鲫鱼体内的血药浓度,分析两者之间的相关性。结果显示,吡喹酮能够迅速进入血液,并被快速消除,药时数据符合二室开放模型。在1 h时,血液中吡喹酮的浓度达到最大值,为2.85?g/m L,96 h后血液中检测不到吡喹酮;灌药后,MPO基因表达量随时间呈先升后降趋势,在1 h时髓过氧化物酶表达量最高。此外,0.25 h、0.5 h、1 h、3 h与6 h组与对照组差异性极显著(P0.01),12 h组与对照组差异显著(P0.05)。48 h与96 h组与对照组差异不显著(P0.05)。相关性分析发现,MPO m RNA相对表达量与血液中吡喹酮的浓度之间相关系数r=0.96,为高度相关,并且推测MPO可能参与吡喹酮的氧化代谢。结论认为:(1)MPO m RNA相对表达量的升高与外源性药物吡喹酮的摄入有关。(2)血液中吡喹酮的残留量与MPO m RNA相对表达量线性相关。本研究旨在提供一种从分子水平评价水产动物体内药物残留的新思路。     

The effects of partial replacement of fishmeal protein by hydrolysed feather meal protein in the diet with high inclusion of plant protein on growth performance,fillet quality and physiological parameters of Pengze crucian carp (Carassius auratus var. Pengze)

Goal of this research is to explore the effects of graded dietary levels of hydrolysed feather meal (HFM) protein as an alternative of fishmeal protein on Pengze crucian carp (Carassius auratus var. Pengze). Five isonitrogenous (35% crude protein) diets were formulated as Control diet and four other diets F15, F30, F45 and F60 wherein 15%, 30%, 45% and 60% fishmeal protein replaced by HFM protein fed for 70 days. The results showed that fish growth was unaffected up to 45% fishmeal protein replacement. Dietary HFM protein could reduce the crude protein composition of body and further affect fillet quality through a significant increase in springiness, gumminess, chewiness and/or resilience. Activity of digestive enzymes (amylase, lipase and tryptase) increased as the fishmeal protein replacement increased, whereas a higher replacement level had negative impacts on absorptive capacity of intestine by decreasing its absorptive area, following by a reduction in concentration of lactate dehydrogenase, total cholesterol, high‐density lipoprotein, low‐density lipoprotein and ammonia in serum. Although intestinal antioxidant parameters were elevated by dietary HFM protein , the content of malondialdehyde in intestine was also positively associated with the protein replacement level. Overall, our results indicate that up to 45% of fishmeal protein could be replaced by HFM protein in diet without significant impact on the growth of Pengze crucian carp, whereas the crude protein content of whole body, fillet quality and oxidative status would significantly be affected when the replacement level of fishmeal protein reaches 30% together with a negative effect on the intestinal histology.     

传统池塘和“跑道”养殖黄金鲫的营养组成分析与评价

为研究不同养殖模式对黄金鲫(Carassius auratus)营养组成和品质的影响,以传统池塘精养和采用池塘内循环流水“跑道”模式养成的黄金鲫为研究对象,分析比较了两种养殖模式下黄金鲫肌肉营养成分、质构和风味物质等指标。结果显示,“跑道”组黄金鲫背肌的粗脂肪含量显著低于池塘组,而粗灰分含量显著高于池塘组(P＜0.05);“跑道”组黄金鲫肌肉的硬度、粘附性和黏性等质构指标与池塘组相比显著提升(P＜0.05)。两种模式下养成的黄金鲫,其肌肉的氨基酸组成无显著差异(P＞0.05),且必需氨基酸评分均高于FAO/WHO的标准。“跑道”组黄金鲫肌肉中不饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸占总脂肪酸的比例均显著高于池塘组,而饱和脂肪酸占比显著低于池塘组(P＜0.05)。“跑道”组黄金鲫肌肉中部分挥发性物质,如壬醛、1-辛烯-3-醇的含量也低于池塘组。分析比较结果表明,采用池塘内循环流水“跑道”养殖模式可以在一定程度上提升黄金鲫的营养品质。     

Effect of prebiotic xylooligosaccharides on growth performances and digestive enzyme activities of allogynogenetic crucian carp (<Emphasis Type="Italic">Carassius auratus gibelio</Emphasis>)

The effect of prebiotic xylooligosaccharides (XOS) on the growth performance and digestive enzyme activities of the allogynogenetic crucian carp, Carassius auratus gibelio, was investigated. XOS was added to fish basal semi-purified diets at three concentrations by dry feed weight: diet 1, 50 mg kg⁻¹; diet 2, 100 mg kg⁻¹; diet 3, 200 mg kg⁻¹, respectively. Twelve aquaria (n = 20) with three replicates for each treatment group (diets 1–3) and control treated without XOS were used. Weights of all collected carp from each aquarium were determined at the initial phase and at the end of the experiment, and the carp survival was also determined by counting the individuals in each aquarium. After 45 days, there were significant differences (P < 0.05) in the relative gain rate (RGR), and daily weight gain (DWG) of diets 1–3 were compared with the control. However, the survival rate was not affected (P > 0.05) by the dietary treatments. For enzymatic analysis, dissection produced a crude mixture of intestine and hepatopancreas of each segment to measure. The protease activity in the intestine and hepatopancreas content of fish in diet 2 (487.37 ± 20.58 U g⁻¹ and 20.52 ± 1.93 U g⁻¹) were significantly different (P < 0.05) from that in the control (428.13 ± 23.26 U g⁻¹ and 12.81 ± 1.52 U g⁻¹) and diet 3 (428.00 ± 23.78 U g⁻¹ and 14.04 ± 1.59 U g⁻¹). Amylase activity in the intestine was significantly higher for diet 2 compared to diet 1 and the control. As for amylase in the hepatopancreas, assays showed higher activity in diet 2 (P < 0.05) compared to the rest.     

高碳水化合物水平日粮对异育银鲫生长、生理、免疫和肝脏超微结构的影响

为了探讨异育银鲫对日粮中高碳水化合物的利用,选用168尾异育银鲫,分成2组,一组为正常对照组,投喂35%碳水化合物(35%CHO)水平日粮;另一组为高糖试验组,投喂50%碳水化合物(50%CHO)水平日粮每组设3个重复。在控温的循环水系统中饲养10周后,测定鱼体生长指标、肌肉成分、肝糖元、血液生化指标和免疫指标。结果表明,高糖组(50%CHO)显著(P<0.05)降低了特定生长率、增重率,但是对饵料系数、肝体比、脏体比、鱼体肌肉成分等没有显著(P>0.05)影响;两组异育银鲫血浆总蛋白、甘油三酯、总胆固醇、血糖和肝糖原含量都没有显著差异(P>0.05);高糖组(50%CHO)肝脏丙二醛含量显著升高(P<0.05)、天门冬氨酸转氨酶显著降低(P<0.05),而肝脏超氧化物歧化酶、总抗氧化能力、丙氨酸转氨酶和碱性磷酸酶都没有显著变化(P>0.05)。试验还对两组异育银鲫肝细胞进行超微结构观察,发现高糖饲料引起了异育银鲫肝细胞中糖原和脂肪的沉积,并对细胞和细胞器的结构产生一定损伤。从本试验结果来看,异育银鲫可以耐受50%饲料糖含量,但是长期摄食50%碳水化合物日粮对其生长不利,并会引起一定程度肝脏功能和结构的损伤。     

壳聚糖和益生菌对异育银鲫非特异免疫功能及血清甲状腺激素、皮质醇水平的影响

为探讨壳聚糖和益生菌是否通过甲状腺激素、皮质醇对异育银鲫的非特异免疫功能的进行调控,试验以基础饲料为对照,在基础饲料中分别添加0.50%壳聚糖A、0.50%壳聚糖B、0.20%益生菌以及0.50%壳聚糖A与0.05%益生菌的混合物制备试验饲料,饲养异育银鲫64d后,测定各组鱼外周血白细胞数量及其分类、白细胞吞噬活性、脾脏溶菌酶活性、脾脏淋巴细胞转化能力;同时采用放射免疫分析法测定异育银鲫血清中三碘甲腺原氨酸(T3)、甲状腺素(T4)及皮质醇含量。结果表明,饲料中添加壳聚糖、益生菌或其混合物对异育银鲫白细胞总数无显著影响,但是可显著提高异育银鲫外周血淋巴细胞数量、白细胞吞噬活性和脾脏溶菌酶活性(P<0.05),显著降低外周血多形核细胞数量(P<0.05)。除了壳聚糖A能够显著提高异育银鲫脾脏B淋巴细胞转化能力之外,其余试验组的脾脏T、B淋巴细胞转化能力均与对照组无显著差异(P>0.05)。同时发现饲料中添加壳聚糖、益生菌或其混合物可以显著提高异育银鲫血清中T3的含量(P<0.05)、显著降低异育银鲫血清中皮质醇的含量(P<0.05),除了壳聚糖B对异育银鲫血清中T4的含量无显著影响(P>0.05)之外,其余均可...     

银鲫肠道内抑制嗜水气单胞菌的潜在益生菌筛选及其特性研究

从养殖的银鲫(Carassius auratus gibelio)肠道中分离纯化得到60株具有不同特征的菌株,以嗜水气单胞菌(Aeromonas hydrophila)为指示菌,点种法初筛得到12株菌株,再通过牛津杯实验筛选出4株对嗜水气单胞菌有明显抑制作用(抑菌圈直径在14 mm以上)的益生菌疑似株,并进行了耐热试验、耐酸试验和人工肠液耐受试验,筛选出最符合益生菌功能特性的菌株YJ-9。该菌株在90℃高温水浴30 min后相对存活率为1.51%,在pH3的液体培养基中4 h内相对存活率为8.37%,活菌数都保持在106 CFU/mL以上,并且可以在人工模拟肠液中缓慢生长。在急性毒力实验中,10倍于实验剂量的菌株YJ-9对银鲫无毒,动物保护实验证明菌株YJ-9能有效增强银鲫对嗜水气单胞菌的免疫抵抗力,相对保护率达到80%。研究结果表明,菌株YJ-9可以作为一个新的饲用微生态制剂候选菌种。     

异育银鲫P450家族CYP3A136基因的克隆与表达

根据5种硬骨鱼已知CYP3A序列保守区设计兼并引物,以异育银鲫cDNA为模板扩增得到CYP3A基因片段,根据得到片段序列设计特异引物并利用cDNA末端快速扩增技术(RACE)获得全长cDNA。得到异育银鲫CYP3A基因全长为1 769 bp,开放阅读框为1 545个核苷酸,编码514个氨基酸。其预测蛋白质分子量为58.624 ku,理论等电点为6.30。将异育银鲫CYP3A序列理论编码氨基酸序列提交细胞色素P450命名委员会(Cytochrome P450 Nomenclature Committee)并由其命名为CYP3A136。氨基酸序列分析显示,其与稀有鮈鲫、草鱼、鲦同源性较高,并且具有高度保守的血红素结合区域FXXGXXXCXG。异育银鲫CYP3A基因的半定量RT-PCR显示,在肝和肠组织中转录水平最高,在肾和鳃中次之,其余组织较低。     

额尔齐斯河2种类型雌性银鲫的形态特征及D-loop基因序列比较

2008年5月调查发现,在额尔齐斯河主河道(185团)的雌性银鲫(Carassius auratus gibelio)群体中存在2种形态类型,按照体长比体高的形态学性状区分,分别为高背型(1.78~2.14)和低背型(2.18~2.39)。形态学分析表明,高背型和低背型的侧线鳞分别为31和30,侧线上(下)鳞为分别7和6,两者在体高、头高、尾柄高、体宽、头宽等性状上存在显著差异(P<0.01),高背型比低背型具有明显的体型优势。生长特性分析表明,在相同体长或年龄的雌性群体中,高背型的平均体质量约为低背型的1.3~1.9倍,而表现出更为优越的生长性能。线粒体控制区序列分析表明,高背型和低背型群体各自独享1个单倍型(分别为EG1和ED1),为2个不同的遗传谱系类群,遗传分化仅为0.004,远低于属内种间的0.020~0.045,两者尚属群体内变异;在分子系统关系中,高背型和低背型分别与方正银鲫的2个单倍型(FZ1和FZ13)共享并聚为单系,推测额尔齐斯河银鲫源自于黑龙江(方正银鲫)。与国内其他地方群体或品种比较表明,额尔齐斯河2种类型雌性银鲫,特别是高背型具有更为突出的生长优势,可作为优良的开发养殖品种和更具潜力的鱼类育种材料。