微生态添加量对商品肉鸡生长性能的影响

选用36000只1日龄罗斯308商品肉鸡,随机分成3个处理组,每个处理3个重复,每个重复4000只鸡。各处理组从1日龄开始至14日龄每天分别饮用绿源生0ml/只(T1)、0.35ml/只(T2)和0.65ml/只(T3)。研究饮水中添加不同剂量绿源生对肉鸡生长性能的影响。结果表明,绿源生有使肉鸡全期日增重线性上升的趋势(P=0.06)。随着绿源生添加剂量的增加,试验第2周(P<0.05)和全期(P<0.01)增重耗料比呈二次曲线上升。饮水中添加绿源生时间少于7d,可能对生产性能没有显著影响,增加饮水中绿源生的添加量能显著改善肉鸡的饲料转化率。     

Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers

1. The effect of probiotic supplementation on egg production, on serum and yolk cholesterol and on egg shell thickness in 24 White Leghorn layers was studied from 28–38 weeks of age.

2. In 3 treatments the diet was supplemented with 0, 100 and 150 mg probiotic/kg food.

3. In the 100 mg probiotic group, egg production improved by 5%, and shell thickness improved slightly, with fewer thin‐shelled eggs than in the control (8.6% compared to 18.6%).

4. The initial serum cholesterol concentration of 170.2 mg/dl in control birds remained similar throughout the 10‐week experimental period, whereas in the 150 mg group the initial value of 176.5 mg/dl decreased to 114.3 mg by week 10.

5. Yolk cholesterol concentration was 14.69 mg in the control group and 11.28 and 11.37 mg/g in the 100 and 150 mg probiotic groups respectively. Overall mean total egg cholesterol was thus reduced by probiotic supplementation.     

Effect of diet form and enzyme supplementation on growth, efficiency and energy utilisation of wheat-based diets for broilers

1. The effect of diet form (mash, cold-pelleted, steam-conditioned/pelleted, wet mash, whole wheat with balancer pellet, restricted pellet) and enzyme inclusion (Avizyme 1300, absent, present) was studied in 2 trials using individually caged, male broilers from 14 to 42 d. Bird performance, viscosity of ileal contents and diet metabolisability (AME) were measured. 2. The performance of mash-fed birds was significantly poorer than for the other treatments in relation to dry matter intake, liveweight gain and gain:food. This was not due to reduced diet AME content. 3. There was no significant effect of heat treatment on any of the variables measured, although viscosity of ileal contents was increased by 30% as compared to the cold-pelleted diet. 4. Gain:food was improved with wet-mash feeding in comparison to the dry mash treatment but it was concluded that this was not due to any intrinsic improvement in diet quality, but rather to voluntary food restriction on introduction of the wet food. 5. Whole wheat feeding improved gain:food and diet AME content by 3% as compared to the complete diets and caused approximately a 50% increase in gizzard weight as compared with the pelleted diets. 6. Food enzyme inclusion did not improve performance although a significant improvement in diet AME content was observed with enzyme inclusion in trial 1.     

Effect of supplementation of multi-microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers

The present study investigated the effect of inclusion of multi-microbe probiotic product on growth performance, apparent digestibility of nutrients, cecal microbiota and small intestinal morphology in broilers. Four hundred days-old Ross chicks were randomly allotted to five treatments on the basis of body weight (BW). Each treatment had four replicates of 20 chicks in each. Experimental diets were fed in two phases, starter (day 0-21) and finisher (day 22-35). Dietary treatments were; basal diet without any antimicrobial (NC), basal diet added with 20 mg Avilamycin/kg of diet (PC), 10(7) cfu multi-microbe probiotic/kg of diet (P1), 10(8) cfu multi-microbe probiotic/kg of diet (P2), and 10(9) cfu multi-microbe probiotic/kg of diet (P3). Overall BW gain and feed conversion ratio were better (p < 0.05) for treatments PC, P2 and P3 compared with NC and P1, with P1 being better (p < 0.05) than NC. Overall feed intake in treatments PC, P1, P2 and P3 were greater (p < 0.05) than NC. Apparent digestibility of dry matter and crude protein were greater (p < 0.05) in treatments PC, P2 and P3 compared with NC, with P1 being intermediate and not different form NC, PC, P2 and P3. At d 21 and 35, treatments PC, P1, P2 and P3 showed lower (p < 0.05) cecal Clostridium and Coliforms count in relation to NC. Moreover, cecal Clostridium (d 21) and Coliforms (d 21 and 35) count were lower (p < 0.05) in treatment PC in relation to P1; with P2 and P3 being intermediate and not different from PC. However, there was no effect of dietary treatments on cecal total anaerobic bacteria and Bifidobacterium spp. count. The villus height of duodenum in treatment PC was greater (p < 0.05) than NC, with P1, P2 and P3 being intermediate. Villus height of ileum in treatment PC was greater (p < 0.05) than in treatments P1 and NC, whereas it remained comparable among treatments PC, P2 and P3. Villus height to crypt depth ratio of ileum was greater (p < 0.05) for treatment PC, P2 and P3 compared with that in P1 and NC. It is concluded that multi-microbe probiotic inclusion at 10(8) and 10(9) cfu/kg diet had beneficial effects on broilers growth performance, apparent digestibility of nutrients and intestinal morphology and can be used as replacement to antibiotics growth promoter in broiler nutrition.     

Effect of probiotic supplementation on growth performance,intestinal morphology,barrier integrity,and inflammatory response in broilers subjected to cyclic heat stress

This study investigated the protective effects of probiotic on heat stress‐induced intestinal injury and inflammatory response in broilers. A total of 180 male broilers were randomly allocated to three treatments with four replicates each from 22 to 42 days of age. The broilers were either raised under thermoneutral (TN) conditions (23 ± 1°C) or subjected to cyclic heat stress (28–35–28°C for 12 hr daily). The broilers kept at TN conditions were fed a basal diet, and those exposed to heat stress were fed basal diets supplemented with or without probiotic at a dose of 1.5 × 10⁸ cfu/kg. Compared with the TN group, heat stress decreased (p < .05) the growth performance, reduced (p < .05) villus height and villus height: crypt depth ratio in intestinal mucosa, increased (p < .05) serum levels of D‐lactic acid on day 28 and endotoxin, TNF‐α and IL‐6 on day 42, and decreased (p < .05) serum IL‐10 content on day 42. Dietary supplementation of probiotic reversed (p < .05) all these changes except for the growth performance in heat‐stressed broilers. In conclusion, dietary inclusion of probiotic could improve intestinal morphology and barrier function, alleviate inflammatory response, but exert no ameliorative effect on growth performance of broilers under cyclic heat stress.     

Effect of fat supplementation on nitrogen utilisation of lambs and nitrogen emission from their manure

The effects of various fats on nitrogen digestion and metabolism of lambs and their manure were investigated in six isoenergetic diets (n = 6) with similar contents of absorbable protein. Treatments were either a control diet or diets containing 25 g/kg of additional ether extract from rumen-protected fat, coconut oil, rapeseed, sunflower seed or linseed. Fat supplementation resulted in trends for higher apparent nitrogen digestibilities (significant with coconut oil; P < 0.05, post hoc test) and body nitrogen retention (P < 0.1). Urinary nitrogen losses and their proportion of manure nitrogen did not differ significantly among groups as was also true for C / N ratio, dry matter, nitrogen and ammonia nitrogen contents of the manure. In the first week of manure storage, most fat supplements tended to decrease gaseous nitrogen emission as assessed by respiration chamber and mass balance measurements. These differences tended to be mitigated after 7 weeks of manure storage. Only the coconut-oil treatment still resulted in numerically lower nitrogen emissions. In conclusion, addition of fat source in the diet, in addition to increasing dietary energy and suppressing methanogenesis, can under certain circumstances decrease gaseous nitrogen emission from the manure.     

Effect of dietary probiotic,prebiotic and synbiotic supplementation on performance,immune responses,intestinal morphology and bacterial populations in broilers

This study was conducted to investigate the effects of probiotic (Primalac), prebiotic (TechnoMos) and synbiotic (Primalac + TechnoMos) supplementation on performance, immune responses, intestinal morphology and bacterial populations of ileum in broilers. A total of 240 one‐day‐old broiler chicks were randomly divided into four treatment groups which included 60 birds. Control group did not receive any treatment. The chicks in the second, third and fourth groups were fed probiotic (0.9 g/kg), prebiotic (0.9 g/kg) and probiotic (0.9 g/kg) plus probiotic (0.9 g/kg; synbiotic), respectively, at entire period. Daily feed intake, daily weight gain and feed conversion ratio were evaluated. The birds were immunized by sheep red blood cell (SRBC) on days 12 and 29 of age and serum antibody titres were measured on days 28, 35 and 42. Newcastle vaccines administered on days 9, 18 and 27 to chicks and blood samples were collected on day 42. Intestinal morphometric assessment and enumeration of intestinal bacterial populations were performed on day 42. The results indicated that consumption of probiotic, prebiotic and synbiotic had no significant effect on daily feed intake, daily body weight gain, feed conversion ratio, carcass traits, intestinal morphology and bacterial populations of ileum (p > 0.05). Consumption of prebiotic increased total and IgM anti‐SRBC titres on days 28 and 42 and antibody titre against Newcastle virus disease on day 42 (p < 0.05). Synbiotic increased only total anti‐SRBC on day 28 (p < 0.05). It is concluded that consumption of prebiotic increased humoral immunity in broilers. Therefore, supplementation of diet with prebiotic for improvement of humoral immune responses is superior to synbiotic supplementation.     

乳化剂对肉仔鸡生产性能及养分利用率的影响

本试验旨在探讨不同来源油脂饲粮中添加乳化剂对肉仔鸡生产性能及养分利用率的影响。试验采用2×3二因子析因试验设计,A因子为菜籽油和牛油两种油脂饲粮组,B因子为无乳化剂添加组、添加乳化剂1组和添加乳化剂2组,乳化剂添加量均为商业推荐量。选取672只1日龄AA肉仔鸡随机分成6组,每组8个重复,每个重复14只鸡(公母各半)。结果表明,肉仔鸡生长后期及全期,乳化剂1组平均日采食量和平均日增重显著高于对照组(P<0.05),乳化剂1和2均有降低料肉比的趋势。饲粮中添加乳化剂1显著提高了生长前期肉仔鸡干物质表观消化率及表观代谢能值(P<0.05)。牛油饲粮组肉仔鸡生长全期的平均日采食量和料肉比显著高于菜籽油饲粮组(P<0.05),不同油脂饲粮组肉仔鸡的平均日增重无显著差异(P>0.05)。菜籽油饲粮组生长全期肉仔鸡粗脂肪表现消化率和表观代谢能值均显著高于牛油组(P<0.05)。结果提示,从试验全期来看,在牛油饲粮和菜籽油饲粮中分别添加外源乳化剂,肉仔鸡生产性能及脂肪利用率均有提高,乳化剂对牛油饲粮的改善程度优于菜籽油饲粮,且乳化剂产品之间也有差异。     

Effect of probiotic on growth performance and digestive enzyme activity of Arbor Acres broilers

The effect of probiotic, Bacillus coagulans NJ0516, on growth performance and digestive enzyme activity of Arbor Acres (AA) broilers was investigated. Strains NJ0516 were added to commercial basal diets as probiotic at three final concentrations: T-1, 1 × 10⁶ cfu g⁻¹; T-2, 2 × 10⁶ cfu g⁻¹ and T-3, 4 × 10⁶ cfu g⁻¹, respectively. Twelve groups, of 30 broilers, with three replicates for each treatment group (T-1, T-2 and T-3) and the control group treated without probiotic were used. After 49 days, broilers receiving the diets supplemented with probiotic showed significantly better growth performances including final weight and daily weight gain (DWG) than those fed the basal diet (control). As for feed conversion ratio (FCR), T-2 and T-3 showed lower value (P < 0.05) than the control. However, there was no significant different in final weight, DWG and FCR between T-1, T-2 and T-3 and the survival rate was not affected (P > 0.05) by the dietary treatments. The higher protease activities were observed in T-2 and T-3 (P < 0.05) compared with the control and T-1. However, there was not significantly different (P > 0.05) between T-2 and T-3 in protease activity. Amylase activity in T-1, T-2 and T-3 was remarkably higher (P < 0.05) than that in the control. Significantly higher amylase activity was observed in T-2 compared with that of T-1. There was no remarkable difference (P > 0.05) in amylase activity of T-2 compared with that of T-3, even though there was a tendency for increased activity. As for lipase activity of duodenum in broilers, assays showed no difference in all treatment groups. It showed that probiotic, B. coagulans NJ0516 administration in feed with a certain concentration displayed a growth promoting effect and increased the protease and amylase activities.     

Effect of stachyose supplementation on growth performance, nutrient digestibility and caecal fermentation characteristics in broilers

1. The objective of this study was to evaluate whether the oligosaccharide stachyose enhances gastrointestinal tract health by fermentation and proliferation of desirable bacteria species and thus affects growth performance and nutrient digestibility in broilers. 2. A total of 432 1-d-old male Arbor Acres (AA) broilers were randomly allocated to one of 6 treatments, with 12 replicate pens per treatment and 6 birds per pen. Chicks were fed a maize-hamlet protein 300 (HP300) basal diet with 0, 4.0, 8.0, 12.0 or 16.0 g/kg stachyose. A sixth diet contained no HP300 but soybean meal (SBM) and provided 8.7 g/kg stachyose and 3.1 g/kg raffinose. The duration of the study was 42 d. 3. Stachyose contents above 12.0 g/kg depressed group body weights, average daily gain and feed/gain but not feed intake during the whole experimental period. Broiler growth decreased linearly and quadratically with increasing stachyose content. No differences were detected between diets supplemented with 12.0 g/kg stachyose and SBM. 4. Nutrient digestibility tended to decrease but not significantly with increasing stachyose. 5. Stachyose content had no significant positive effects on caecal pH, microflora population and the resulting short-chain fatty acid (SCFA) metabolites during the 42 d experiment, with only butyrate differing significantly in the initial period.     

Influence of probiotic supplementation on immune response in broilers raised under hot climate

1. The objective of the study was to evaluate the potential effects of dietary probiotic supplementation and stocking density and their interaction on growth performance, humoral and cellular immune responses of broiler chickens raised under hot climate conditions.

2. A total of 1800 1-d-old unsexed broiler chicks (Ross 308) were allocated in a completely randomised 3 × 2 factorial design, with three concentrations of dietary probiotic (0, 200 and 400 mg/kg) containing 4 × 10⁹ cfu/g of Bacillus subtilis and two stocking densities (12 or 18 birds/m²) in a total of 6 treatment subgroups.

3. Dietary probiotic and stocking density had no significant effect on live body weight, feed consumption and feed conversion ratio.

4. Dietary probiotic supplementation had a positive effect on serum immunoglobulin M (IgM) and cell-mediated immunity when compared to the control at 6 weeks of age, whereas serum immunoglobulin A (IgA) and immunoglobulin Y (IgY) were improved, but not significantly so. Dietary probiotic and stocking density had no effect on relative weights of the bursa of Fabricius and spleen.

5. Dietary probiotic supplementation had a positive significant effect on serum IgM concentration and cell-mediated immunity without penalising growth performance in broilers raised under high ambient temperature.     

伊普异黄酮对不同生长阶段肉鸡生长性能和有关血清生化指标的影响

选用1日龄艾维茵肉鸡100羽,随机分成2组,试验组在基础日粮中添加伊普异黄酮5 mg/kg。试验开始3周后宰杀40羽,6周后宰杀剩余的60羽,研究伊普异黄酮对不同生长阶段肉鸡生长性能的影响。结果显示,添喂伊普异黄酮分别使生长前期公鸡和母鸡增重提高2.21%、0.95%,血清尿酸含量降低27.06%(P<0.05)、1.06%,血清T3水平升高12.4%、9.67%,血清FT3水平升高56.51%(P<0.05)、7.06%;使整个生长期公鸡和母鸡增重提高10.73%(P<0.05)、5.86%,血清尿酸含量降低35.11%(P<0.05)、14.63%,血清T3水平升高42.56%(P<0.05)、73.24%(P<0.05),血清FT3水平升高126.56%(P<0.01)、85.88%(P<0.05),同时血清碱性磷酸酶活性明显升高,血清钙、总胆固醇、甘油三酯含量均明显降低。     

微生态制剂对肉鸡代谢和免疫系统的影响

本研究旨在观察一种由多种有益菌组成的微生态制剂（主要成分为乳酸杆菌和芽孢杆菌等）通过饮水摄入以后,对鸡舍氨气浓度,肉鸡血清中血氨、血清尿酸、总蛋白、白蛋白和球蛋白含量和免疫系统的影响。选用240只1日龄AA肉鸡,按试验需求随机分成4个试验组,即对照组、试验1组、试验2组和试验3组。每个试验组3次重复,每次重复20只。从1日龄开始,对照组按正常饮水,其余各组均在饮水中添加该液态微生态制剂,添加量分别为试验1组0.3%,试验2组0.6%,试验3组1.2%。结果表明,试验2组在第6周可显著降低鸡舍内的氨气浓度（P〈0.05）。各试验组血清总蛋白、球蛋白含量均显著高于对照组（P〈0.05）。试验1组肉鸡免疫后的抗体水平显著高于对照组（P〈0.05）。结果提示,适量添加该微生态制剂能在一定程度上降低鸡舍氨气浓度,使机体血液中总蛋白、球蛋白含量升高。同时可以增强机体体液免疫能力,但对细胞免疫的影响效果不显著。     

糖肽酮萜素对肉仔鸡生产性能、免疫器官和血液生化指标的影响

选1日龄艾维茵肉仔鸡600只,随机分成3组(每组4个重复,每个重复50只)。空白对照组饲喂基础日粮(无抗生素添加剂),抗生素对照组饲喂基础日粮+380mg/kg金霉素,试验组饲喂基础日粮+500mg/kg糖肽酮萜素,试验期为49d。饲养试验结果表明:在1～49日龄期间,试验组比空白对照组、抗生素对照组的肉仔公鸡平均日增重分别提高了17.42%(P<0.05)、0.92%(P>0.05);而料肉比分别降低了6.06%(P<0.05)、1.06%(P>0.05)。对于肉仔母鸡,与空白对照组相比,试验组日增重提高了12.68%(P<0.05),料肉比降低了7.21%(P<0.05);日增重及料肉比比抗生素对照组略低,但差异不显著(P>0.05)。试验结果还表明,饲料中添加糖肽酮萜素可以明显提高免疫器官指数(P<0.05)。对血清生化指标的测定结果表明,糖肽酮萜素组鸡的血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇含量比抗生素对照组和空白对照组均有明显降低(P<0.05);而高密度脂蛋白胆固醇含量却反映出相反的趋势,试验组比对照组有了明显提高(P<0.05)。试验组的血氨浓度、尿酸含量显著低于空白对照组(P<0.05),与抗生素组差别不显著(P>0.05)。本研究结果证明:糖肽酮萜素完全可以替代抗生素类饲料添加剂。     

Effect of cereal type and Xylanase supplementation on nutrient retention and growth performance of broilers

An experiment was conducted to study the effect of cereal (corn, wheat, barley and sorghum) and supplemental enzyme (a mono-component xylanase) in a 4 × 2 factorial arrangement. A 2-phase feeding program (0 to 21 and 21 to 42 d) was used and 4 iso-caloric, iso-nitrogenous diets were formulated containing corn, wheat, barley, or sorghum as the sole cereal. Diets based on each cereal were fed without or with supplemental xylanase (16,000 BXU/kg) to 8 replicate pens of 10 chicks (5 male : 5 female, Cobb 500) each. Growth performance was recorded at 21 or 42 d posthatch. Excreta was quantitatively collected from 18 to 21 and 38 to 41 d for the measurement of the total tract retention of dry matter (DM), nitrogen (N) and neutral detergent fiber (NDF) and apparent metabolizable energy (AME). Ileal digestible energy (IDE) was measured at the end of the study (42 d posthatch) using titanium dioxide as an indigestible marker. For the overall 42 d period, birds fed barley-based diets had lower feed intake (P < 0.05), lower body weight (P < 0.05) and converted feed into gain less efficiently (P < 0.05) compared with the diets based on corn, wheat or sorghum. Xylanase supplementation improved weight gain in diets based on corn, wheat, and sorghum with the exception of barley-based diets (cereal × xylanase interaction, P < 0.05). Xylanase improved the overall feed conversion ratio (1.885 vs. 1.939; P < 0.05) with the effect being independent of the cereal type. The N retention of barley-based diets was lower (P < 0.05) compared with the other cereals, while xylanase improved N retention (P < 0.05) regardless of the cereal type. NDF digestibility differed (P < 0.05) across cereal (barley > wheat > corn > sorghum) and was improved (P < 0.05) by xylanase supplementation. A significant cereal × xylanase interaction (P < 0.05) was observed for energy measurements, where xylanase improved IDE of the corn-based diet, and AME of corn- and wheat-based diets. Results of the current study demonstrate potential of xylanase in improving nutrient retention and growth performance of broilers fed diets based on variety of cereal grains.     

Effect of Melissa officinalis supplementation on growth performance and meat quality characteristics in organically produced broilers

1. A trial was conducted to study the effect of Melissa officinalis supplementation on organic broiler performance and meat chemical, microbiological, sensory and nutritional quality.

2. Male and female day-old Ross 308 chicks were fed on a standard commercial diet containing 0, 2.5, 5 or 10 g/kg feed ground M. officinalis for 84 d before slaughter.

3. Weight gain and feed conversion ratio were significantly improved in the broilers receiving either 5 or 10 mg M. officinalis/kg feed.

4. Inclusion of M. officinalis did not affect muscle chemical and fatty acid composition.

5. On the basis of microbiological and sensory experimental data and subsequent extension of meat shelf life, M. officinalis did not reduce the microbial populations of the meat, but was effective in limiting lipid oxidation.     

Effect of spray-dried bovine serum on intake, health, and growth of broilers housed in different environments

Three experiments utilizing broilers were conducted in different environments to evaluate the effects of Innavax (INX; spray-dried serum) administered in drinking water on broiler performance. In Exp. 1 (1 to 42 d), 252 Ross x Cobb male broilers were assigned randomly to one of six treatments consisting of tap water mixed with 0, 0.25, 0.50, 0.75, 1.0, or 1.25% (wt/wt) INX. Broilers (six broilers per pen; seven pens per treatment) were housed in Petersime battery cages (raised wire flooring) in temperature-controlled rooms. Average daily gain, and feed and water intake (as-fed) were not affected (P > 0.05) by experimental treatments. Feed efficiency tended to improve linearly (P = 0.076) from d 0 to 7 with increasing levels of INX, but was unaffected (P > 0.05) during the remaining periods. In Exp. 2 and 3, 800 Ross x Ross 308 male broilers (400 broilers in each trial; 10 broilers per pen; 10 pens per treatment) in two 21-d experiments were assigned randomly to one of four treatments consisting of tap water mixed with 0, 0.45, 0.90, or 1.35% (wt/wt) INX. Broilers were housed in floor pens containing clean (Exp. 2) or used (Exp. 3) litter. In Exp. 2, intake, ADG, and feed efficiency were linearly improved (P < 0.05) during the first week with increasing levels of INX. During the second week (d 8 to 14), ADG, water intake, and feed efficiency were linearly improved (P < 0.05) with increasing levels of INX. In the third week (d 15 to 21), ADG and feed and water intake were not affected (P > 0.10) by level of INX. Overall (d 0 to 21), ADG, intake, and feed efficiency were linearly improved (P < 0.05) with INX. In Exp. 3, ADG, water intake, and feed efficiency were linearly improved (P < 0.05) during each period. Feed intake was not affected (P > 0.05) by experimental treatment during d 0 to 7, but was linearly increased (P < 0.05) from d 8 to 14 and 15 to 21. The greatest growth response of broilers to INX was observed when broilers were housed in floor pens with used litter, followed by floor pens with clean litter and battery pens. Further research on the relationship between the response to INX and housing conditions seems warranted.