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甘蔗花叶病广泛存在于我国甘蔗种植区,严重影响甘蔗产业的高质量发展。近年来甘蔗线条花叶病毒在蔗区肆虐,尽管针对其的血清学检测技术已经建立,但是快速、准确、高通量的检测方法亟待发掘。本研究制备了SCSMVCP的抗血清,特异性高,与引起甘蔗花叶病的另两种病原 (高粱花叶病毒和甘蔗花叶病毒) 间没有血清学交叉反应。基于该多克隆抗体,建立了直接抗原包被的ELISA、斑点杂交、Western blot和基于多抗的免疫试纸条检测技术。开发的免疫试纸条检测技术能快速、准确、高通量应用于田间病毒鉴定。本文提供了基于血清学的快速、准确、高通量,且便捷的甘蔗线条花叶病毒检测技术,有助于我国蔗区甘蔗花叶病的监测与防控。 相似文献
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《植物病理学报》2020,(4)
甘蔗花叶病广泛存在于我国甘蔗种植区,严重影响甘蔗产业的高质量发展。近年来甘蔗线条花叶病毒在蔗区肆虐,尽管针对其的血清学检测技术已经建立,但是快速、准确、高通量的检测方法亟待发掘。本研究制备了S CSMV CP的抗血清,特异性高,与引起甘蔗花叶病的另两种病原(高粱花叶病毒和甘蔗花叶病毒)间没有血清学交叉反应。基于该多克隆抗体,建立了直接抗原包被的ELISA、斑点杂交、Western blot和基于多抗的免疫试纸条检测技术。开发的免疫试纸条检测技术能快速、准确、高通量应用于田间病毒鉴定。本文提供了基于血清学的快速、准确、高通量,且便捷的甘蔗线条花叶病毒检测技术,有助于我国蔗区甘蔗花叶病的监测与防控。 相似文献
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基于PCR技术的植物病原真菌检测技术研究进展 总被引:4,自引:0,他引:4
植物病原真菌是可以在植物上引起病害的一类重要病原物,对该类病原的快速检测是对其进行植物检疫、监测预报及病害防治必不可少的基础工作.近年来,以PCR为基础的分子检测技术的日益发展使得植物病原真菌的检测更加快速、灵敏和可靠.本文对近年来基于PCR技术的植物病原真菌检测方法进行了介绍与评述,这些方法包括ITS-PCR、巢式PCR、多重PCR、PCR ELISA和实时荧光定量PCR技术. 相似文献
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斑马片病是一种可对马铃薯等茄科作物造成严重危害的病害,其病原为Candidatus Liberibacter solanacearum,主要通过马铃薯木虱(Bactericera cockerelli)等介体进行传播。该病害主要分布于美国、墨西哥、中美洲各国和新西兰等国家,虽然目前中国没有发生的报道,但具备该病害传入和发生的条件。本文对斑马片病的发生发展过程,病原基因组学、分子检测技术、防控措施及对我国的风险等进行讨论,对于全面了解该病害,防范其传入我国并在我国流行具有重要意义。 相似文献
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2019年10月,在云南弥勒甘蔗示范基地(23.92°N,103.33°E)发现‘云瑞10-187’和‘福农11-2907’高感甘蔗褐条病,发病率为50%~80%。为明确其病原,本研究采集病样进行了病原菌的分离、鉴定及系统发育分析,以期为该病害有效防控提供科学依据。依据形态特征、核糖体RNA的内转录间隔区(internal transcribed spacer, ITS)和甘油醛-3-磷酸脱氢酶基因(glyceraldehyde-3-phosphate dehydrogenase, GAPDH)分子鉴定及致病性测定,将病原菌鉴定为狗尾草平脐蠕孢Bipolaris setariae,是云南省甘蔗褐条病病原菌新记录种,丰富了甘蔗褐条病病原菌信息,为后续其他蔗区褐条病的研究奠定了基础。 相似文献
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由白条黄单胞杆菌Xanthomonas albilineans引起的甘蔗白条病是一种寄生在植物维管组织的系统性细菌病害,在全球多数种植甘蔗的国家或地区普遍发生,对甘蔗产业的发展构成潜在威胁。本研究综述了甘蔗白条病的发生和分布、传播与流行规律以及病原菌生物学与基因组特性、鉴定与检测、遗传多样性和致病机理等方面内容;提出加强抗病种质挖掘与新品种选育推广、加快抗病分子育种进程、切断病害传播途径、加强隔离检验检疫等病害防控策略;此外,评估了该病害在我国蔗区流行暴发的风险,展望今后甘蔗抗病分子育种、病原菌致病机制及其与寄主互作机理等方面的分子基础研究重点与应用前景。 相似文献
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在广西博白县发现一种国内外尚未报道的新病害-甘蔗心腐病。根据对病原细菌的个体形态、培养性状、生理生化反应及其对菠萝果实的致病性等方面的试验研究结果,鉴定甘蔗心腐病病原细菌为菠萝欧文氏杆菌(Erwinia ananas Serrano)。 相似文献
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甘蔗是最重要的糖料作物,由于其栽培过程中采用种茎无性繁殖,病毒病发生逐年加重.已知侵染甘蔗的病毒种类有甘蔗花叶病毒(Sugarcane mosaic virus,SCMV)、高粱花叶病毒(Sorghummosaic virus,SrMV)、甘蔗线条花叶病毒(Sugarcane streakmosaic virus,SCSMV)、甘蔗黄叶病毒(Sugarcane yellow leaf virus,SCYLV)、甘蔗斐济病病毒(Sugarcane Fiji disease virus,SFDV)、甘蔗线.条病毒(Sugarcane streak virus,SSV)和甘蔗杆状病毒(Sugarcane bacilliform virus,SCBV).文中简要介绍上述几种病毒的基本特性及其所致病害的发生特点,对目前甘蔗病毒病防治技术进行了评述,提出了我国甘蔗病毒研究中需要关注的若干问题. 相似文献
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马铃薯干腐病是马铃薯最重要的贮藏期病害之一,现已成为马铃薯贮藏期烂窖的重要原因。实现病原菌的快速检测对病害诊断和科学防控具有重要的实践意义。本研究基于镰刀菌翻译延伸因子序列设计了一对检测接骨木镰刀菌Fusarium sambucinum的特异引物Fs-F/Fs-R。该特异引物可从接骨木镰刀菌中获得309bp的特异性扩增片段,而其他种类镰刀菌及马铃薯重要病害病原菌中均无此片段,说明该引物具有专一性。该体系的灵敏度检测结果表明,最低能检测到的接骨木镰刀菌基因组DNA浓度为70pg/μL。该引物也适用于发病马铃薯块茎中接骨木镰刀菌的快速检测。 相似文献
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几种常用植物病原细菌分子检测方法 总被引:3,自引:0,他引:3
植物病原细菌(phytobacteria)是植物上一类重要的病原菌,这些细菌能引起多种农作物、经济作物、花卉、树木及牧草上的病害。它的快速检测是病害防治、预测预报及植物检疫必不可少的重要工作。其中,以PCR为基础的分子检测技术的进步使植物病原细菌的检测更快速、灵敏和可靠。本文对近年来植物病原细菌分子检测技术进行介绍,尤其是应用广泛的ITS-PCR(intergenictranscribedspace-PCR)、ARDRA(amplifiedribosomalDNArestric-tionanalysis-PCR)、rep-PCR(repetitiveDNA-PCR)和实时荧光定量PCR(real-timequantitativePCR)技术,旨在促进我国植物病原细菌研究的快速发展。 相似文献
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在推进现代甘蔗产业过程中,有效防控甘蔗病虫害是"双高"甘蔗栽培技术的一个重要环节。目前世界上已发现的甘蔗病害有120种以上,甘蔗害虫上百种,不同国家、不同蔗区甘蔗病虫害种类不同,病菌生理小种、病毒株系也不相同,而许多重要的甘蔗病虫害都是通过种苗传播的。提高对潜在的检疫性甘蔗有害生物认识,有效地阻止危险性病虫随种苗传播蔓延,增强减灾防灾能力,对确保甘蔗品种质量和甘蔗生产安全,促进甘蔗种植业和蔗糖产业持续稳定健康发展具有重要意义。本文重点介绍了几种潜在的检疫性甘蔗有害生物,并提出了相应的防控对策。 相似文献
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Sugarcane yellow leaf virus and sugarcane yellows phytoplasma: elimination by tissue culture 总被引:6,自引:1,他引:6
Yellow leaf syndrome (YLS) is a recently reported disease of sugarcane, characterized by yellowing of the leaves. Two pathogens: a virus, Sugarcane yellow leaf virus (SCYLV); and a phytoplasma, sugarcane yellows phytoplasma (SCYP), are associated with the disease. The use of tissue culture was investigated as a means to eliminate both SCYLV and SCYP from exotic varieties undergoing quarantine in Mauritius. Of 43 varieties in quarantine, 28 were infected with SCYLV and 19 with SCYP when checked by RT–PCR and nested PCR, respectively. Seventeen varieties were coinfected with both pathogens. Thirty infected varieties were induced to form callus in vitro using leaf rolls as explants. After two subcultures, 19 varieties were successfully regenerated and tested for SCYLV and SCYP. No pathogen could be detected in any regenerated plantlets. All the regenerated plants remained free from both SCYLV and SCYP over a period of 1 year in the glasshouse, confirming that the pathogens had been eliminated by tissue culture. 相似文献
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Production of disease-free propagation material is a major means of controlling most bacterial diseases of plants, particularly when neither resistant clones nor effective chemical treatments are available. For this purpose sensitive, specific and rapid detection methods are required. The advent of molecular biology and, in particular, the polymerase chain reaction (PCR) has opened new ways for the characterization and identification of plant pathogens and the development of disease-management strategies. PCR-based detection methods rely on the development of primers for the specific detection of the pathogen. The use of pathogenicity genes as targets for primer design is the preferred procedure for obtaining specific primers but other procedures may also be useful for this purpose. In the present review we describe four examples of procedures for detecting four important bacterial pathogens in Israel: Erwinia herbicola pv gypsophilae in gypsophila, Xanthomonas campestris pv pelargonii in geranium, Agrobacterium tumefaciens in asters and roses, and Xanthomonas campestris pv campestris in crucifers. Procedures for constructing specific PCR primers for each bacterium are illustrated and discussed as well as the combination of PCR with other methods. 相似文献
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M.C. Goncçalves M.M. Klerks M. Verbeek J. Vega J.F.J.M. van den Heuvel 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(5):401-407
Sugarcane yellow leaf virus (ScYLV) is widely distributed in Brazil and other sugarcane producing countries causing significant yield losses. Due to the high incidence of the aphid vector, the virus is widespread in the field and in parental clones used in sugarcane breeding programmes. Aiming to present a sensitive and reliable detection of ScYLV, we have adapted an AmpliDet RNA system, compared it with the currently available detection methods and discussed its applicability for routine diagnosis. AmpliDet RNA consists of nucleic acid sequence-based amplification (NASBA) of the target RNA with specific primers and simultaneous real-time detection of the amplification products with molecular beacons. The results showed that the system produced a detection level of at least 100fg of purified virus. Virus was readily detected in plant tissues with low levels of infection (without the need of previous RNA extraction) and in the hemolymph of aphids. The method showed to be virus-specific, testing negative for other species of the Luteoviridae. In conclusion, the system has potential to become a diagnostic method for the detection of sugarcane viruses. 相似文献
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Bin Li Baoping Liu Changlin Shan Muhammad Ibrahim Yihan Lou Yangli Wang Guanlin Xie Hong‐ye Li Guochang Sun 《Pest management science》2013,69(2):312-320
BACKGROUND: Bacterial leaf blight and leaf streak are the two most damaging bacterial diseases of rice. However, few bactericidal chemicals are available for controlling both diseases. The antibacterial properties of two kinds of chitosan with different molecular weights and degrees of N‐deacetylation and their effect on rice bacterial leaf blight and leaf streak were evaluated. RESULTS: Results showed that the two kinds of chitosan solution possess a strong antibacterial activity against both rice bacterial pathogens and significantly reduced disease incidence and severity by comparison with the control under greenhouse conditions. However, the interaction between chitosan and rice pathogens was affected by the type and concentration of chitosan, the bacterial species and the contact time between chitosan and bacteria. The direct antibacterial activity of chitosan may be attributed to both membrane lysis and the destruction of biofilm. In addition, both chitosan solutions significantly increased the activities of phenylalanine ammonia lyase, peroxidase and polyphenol oxidase in rice seedlings following inoculation of two rice pathogens by comparison with the control. CONCLUSION: The role of chitosan in protection of rice against bacterial pathogens has been shown to involve direct antibacterial activity and indirect induced resistance. Copyright © 2012 Society of Chemical Industry 相似文献