Impact of sodium cyanide on catalase activity in the freshwater exotic carp, Cyprinus carpio (Linnaeus)

The Cyprinus carpio fingerlings on exposure to lethal (1 mg/L) and sub lethal concentrations (0.066 mg/L) of sodium cyanide showed inhibition in the activity of catalase. The disruption of catalase activity in freshwater fish, C. carpio is demonstrated in the present study using UV–visible spectrophotometer at 240 nm using hydrogen peroxide as a substrate. It suggests toxic effects of sodium cyanide and consequent accumulation of hydrogen peroxide in the functionally different tissues namely, liver, gill, muscle and brain. This might lead to cellular damages, and create widespread physiological disturbance. The results suggest that catalase activity can be a good diagnostic tool for sodium cyanide toxicity in biomonitoring programme.     

Effect of short term exposure of fenvalerate on biochemical and haematological responses in Labeo rohita (Hamilton) fingerlings

Experiment was carried out to determine the median lethal concentration (LC₅₀) of fenvalerate to Labeo rohita fingerlings. After determining the LC₅₀ value of fenvalerate, a sub-lethal concentration (1/3rd of LC₅₀) of fenvalerate was exposed for 15 days. Significant alterations in SOD (P < 0.05) activity of liver and gill was observed due to fenvalerate. Catalase activity in gills of fishes was also affected significantly (P < 0.05). WBC, NBT and Hct values were reduced significantly in fenvalerate exposed fishes as compared to control group, whereas blood glucose level showed higher values in fenvalerate exposed group. Serum total protein and albumin were also reduced significantly as a result of fenvalerate exposure. Significant increase in the serum GOT, serum GPT, creatinine, triglyceride and serum ACP was noticed after 15 days of fenvalerate exposure. Results indicated that short term exposure of fenvalerate can induce biochemical and haematological alterations causing stress to L. rohita fingerlings.     

Endocrine disruption and metabolic changes following exposure of Cyprinus carpio to diethyl phthalate

Diethyl phthalate (DEP) enter into aquatic environment from industries manufacturing cosmetics, plastic and many commercial products and can pose potential fish and human health hazard. This experiment evaluated effects of DEP in adult male (89 g) common carp (Cyprinus carpio) by exposing them to fractions of LC₅₀ (1/500-1/2.5) doses with every change of water for 28 days. Vitellogenin induction metabolic enzymes, somatic indices and bioaccumulation were studied on 7th, 14th, 21st and 28th day. The 96th hour LC₅₀ of DEP in fingerlings was found to be 48 mg/L. Compared to control, except increase (P < 0.01) in alkaline phosphatase activity (EC 3.1.3.1) and liver size, there was decrease (P < 0.01) in activity of acid phosphatase (EC 3.1.3.2), aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2) and testiculosomatic index following exposure to 1, 5 and 20 ppm DEP. Significant (P < 0.01) dose dependant vitellogenin induction was observed with exposure of fish to 0.1, 1 and 5 ppm DEP. The bioaccumulation of DEP in testis, liver, brain, gills and more importantly in muscle tissues of fish increased significantly (P < 0.01) with increase of dose from 1 to 5 ppm. Significant interaction (P < 0.01) of dose and duration of exposure indicated that exposure period of a week to two was sufficient to bring about changes in quantifiable parameters studied. Fish exposed to 20 ppm DEP became lethargic and discolored during onset of the 4th week. This is the first report describing metabolic changes and vitellogenin induction following exposure of C. carpio to DEP dose that is as low as 1/500th fraction of LC₅₀.     

在小檗碱作用下水稻细菌性条斑病菌生理及转录反应分析

为探究小檗碱对水稻细菌性条斑病菌Xanthomonas oryzae pv. oryzicola的抑菌机制,测定小檗碱对水稻细菌性条斑病菌GX13菌株的毒力、致病因子、细胞结构和能量代谢的影响,并利用Illumina-HiSeq高通量测序技术分析小檗碱对水稻细菌性条斑病菌菌体基因表达的影响。结果表明:经15μg/mL小檗碱处理后,GX13菌株侵染水稻品种玉丝占后引起水稻细菌性条斑病的病情指数为6.70,低于对照组;每1×108CFU菌体胞外多糖产量为1.58μg,显著低于对照的2.42μg;纤维素酶、蛋白酶、淀粉酶等胞外酶活性均低于对照组;与能量代谢相关的苹果酸脱氢酶(MDH)活性为1.42 U/g prot,低于对照组,而丙酮酸和三磷酸腺苷含量升高,分别为1.71μmol/mL和379.49μmol/g prot,均高于对照组。同时,细菌的生物膜形成受到抑制,细胞结构受到破坏。转录反应分析发现,与II型分泌系统相关的xpsD、xpsF和xpsM等基因、与III型分泌系统相关的hrcC、hrcJ、hrcN和hrcQ等基因、与胞外多糖分泌相关的gumF和gumI基因,以及与细胞结构与功能...     

蠋蝽对棉铃虫幼虫的捕食功能反应

为明确蠋蝽Arma custos对棉铃虫Helicoverpa armigera幼虫的生物防控潜能,在室内条件下测定蠋蝽5龄若虫和雌、雄成虫对棉铃虫3龄幼虫的捕食能力以及不同蠋蝽密度对自身捕食作用的影响。结果显示:蠋蝽5龄若虫和雌、雄成虫对棉铃虫3龄幼虫的捕食功能反应均符合HollingⅡ模型。蠋蝽5龄若虫对棉铃虫3龄幼虫的瞬时攻击率、处理时间和日最大捕食量分别为0.829、0.024 d和41.667头;蠋蝽雌成虫对棉铃虫3龄幼虫的瞬时攻击率、处理时间和日最大捕食量分别为0.796、0.020 d和50.000头;蠋蝽雄成虫对棉铃虫3龄幼虫的瞬时攻击率、处理时间和日最大捕食量分别为0.752、0.021 d和47.619头。蠋蝽5龄若虫和雌、雄成虫对棉铃虫3龄幼虫的搜寻效应均随猎物密度的增加而逐渐降低。当棉铃虫幼虫密度固定时,单头蠋蝽的日捕食量随蠋蝽密度的增加而逐渐降低,蠋蝽对棉铃虫的捕食作用受到自身密度的干扰,5龄若虫和雌、雄成虫的干扰系数分别为0.548、0.702和0.772。表明蠋蝽5龄若虫及雌、雄成虫均对棉铃虫3龄幼虫具有一定的防控能力,且成虫对棉铃虫幼虫的防控能力大于5龄...     

短翅蚜小蜂对桃蚜的取食和寄生功能反应

为评估短翅蚜小蜂对桃蚜的搜寻及控害潜能,采用叶碟法研究了短翅蚜小蜂对甘蓝和辣椒2种植物上桃蚜2龄若虫的取食与寄生行为。结果表明,短翅蚜小蜂对甘蓝桃蚜和辣椒桃蚜的取食和寄生功能反应均符合Holling II及Holling III型方程。短翅蚜小蜂的取食和寄生搜寻效应均随蚜虫密度的增加而降低,当桃蚜密度小于35头时,短翅蚜小蜂对甘蓝桃蚜的取食搜寻效应大于辣椒桃蚜,而当桃蚜密度大于35头时,短翅蚜小蜂对辣椒桃蚜的取食搜寻效应大于甘蓝桃蚜;短翅蚜小蜂在所有密度下对辣椒桃蚜的寄生搜寻效应均大于甘蓝桃蚜。短翅蚜小蜂取食甘蓝桃蚜的瞬间攻击率a'为0.3289,处理时间T_h为0.2597,均大于辣椒桃蚜,寄生甘蓝桃蚜的瞬时攻击率a'为0.8213,小于辣椒桃蚜,而处理时间T_h为0.0275,大于寄生辣椒桃蚜;短翅蚜小蜂对甘蓝桃蚜的理论最大取食量和寄生量均小于辣椒桃蚜。研究表明,短翅蚜小蜂对不同密度的桃蚜均有一定的控制能力,且对辣椒桃蚜的控制作用大于甘蓝桃蚜。     

A juvenile hormone analog, pyriproxifen, affects some biochemical components in the hemolymph and fat bodies of Eurygaster integriceps Puton (Hemiptera: Scutelleridae)

Endocrine system has a critical role during the developmental stages of insects by synthesis of several regulatory hormones. One of these hormones is juvenile hormone that several insecticides have been driven based on its biochemical structure e.g. pyriproxifen. Due to various disadvantages of fenitrothione spraying, this study was carried out finding the possible usage of pyriproxifen to control the destructive population of Eurygaster integriceps. After bioassay treatments to acquire the appropriate concentrations, the treatment repeated to find possible changes in the biochemical compounds of hemolymph and reserved macromolecules in fat bodies. Results showed significant discrepancies in amount of biochemical components of the hemolymph and the reserved macromolecules in E. integriceps after pyriproxifen treatment. In hemolymph, activity level of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase as enzymatic components and uric acid as non-enzymatic one increased but acid phosphatase, lactate dehydrogenase, protein, trehalose and lipid showed adverse results. In fat bodies, the amount of all measured reserves including glycogen, lipid and protein decreased and showed significant differences. These kinds of changes have been supported by several studies due to using insecticides. These negative effects on overall physiology of the Sunn pest by depleting the essential compounds cause sensitivity to fungal infections and several shortages for normal development and reproduction of insect. Also, the adaptability of pyriproxifen to increase the effect of entomopathogenic fungus, Beauveria bassiana, should be considered to initiate a new pest management program to decrease the production loss made by E. integriceps in wheat fields.     

注射大肠杆菌后草地贪夜蛾高龄幼虫的免疫应激反应

为采用生物技术防控草地贪夜蛾Spodoptera frugiperda的扩散为害,对草地贪夜蛾5龄和6龄幼虫注射浓度为1×109CFU/mL的大肠杆菌Escherichia coli菌液,并以注射等量磷酸盐缓冲液(phosphate buffer solution,PBS)和未做任何处理(CK)为对照,24 h后测定幼虫体重、集结数和酚氧化酶(phenoloxidase,PO)活性。结果显示,注射大肠杆菌菌液24 h后,草地贪夜蛾5龄和6龄幼虫体重均受到抑制,其体重分别为0.170 g和0.411 g,均显著低于CK的0.181 g和0.484 g;注射大肠杆菌菌液24 h后,草地贪夜蛾5龄和6龄幼虫集结数分别为135.0、338.4个索引集结数(the indexed nodules,INs),前者极显著低于后者,且均显著高于CK和PBS处理,分别为0.4、10.2个INs和0.3、10.9个INs;注射大肠杆菌菌液24 h后,草地贪夜蛾5龄幼虫PO活性为0.156 ABS·min-1·mg-1,显著高于CK和PBS处理,分别为0.046 ABS·min-1·mg-1和0.066 ABS·min-1·mg-1,但草地贪夜蛾6龄幼虫的PO活性为0.050 ABS·min-1·mg-1,显著低于CK和PBS处理,分别为0.066 ABS·min-1·mg-1和0.069 ABS·min-1·mg-1,且草地贪夜蛾5龄幼虫的PO活性显著高于6龄幼虫的PO活性。表明细菌侵染后草地贪夜蛾不同高龄幼虫的免疫应激反应存在差异,而这种差异可能受幼虫生长发育及细胞免疫和体液免疫功能之间权衡现象的影响。     

普通烟对烟粉虱取食及其与TYLCCNV共侵染的生理生化反应

为明确普通烟遭受烟粉虱Bemisia tabaci取食及与中国番茄黄化曲叶病毒(Tomato yellow leaf curl China virus,TYLCCNV)共侵染时的响应机制,采用常规生理生化指标测定法分析比较MED隐种烟粉虱取食及其与TYLCCNV共侵染处理对普通烟植株中营养组分、防御物质积累及主要氧化酶活性的影响。结果显示,与对照植株相比,烟粉虱取食及其与TYLCCNV共侵染均可导致普通烟植株中可溶性糖和可溶性蛋白含量明显降低,多酚含量显著升高,过氧化氢酶(CAT)活性显著升高,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性呈现先升后降的变化趋势。与烟粉虱取食植株相比,烟粉虱与TYLCCNV共侵染后1 d时普通烟植株中可溶糖含量显著降低了21.05%,3、7和9 d时可溶性蛋白含量分别显著降低了27.40%、26.84%和20.90%;共侵染后的1、3、5和9 d时多酚含量分别显著降低了34.60%、14.30%、3.07%和15.19%;共侵染后的1、3、5和7 d时CAT活性分别提高了3.04倍、1.42倍、1.68倍和1.96倍;共侵染后7 d时POD活性显著降低,共侵染后9 d时SOD活性显著升高。表明普通烟可通过改变其营养组分及积累防御物质或提高氧化酶活性来防御烟粉虱取食及其与TYLCCNV的共侵染,而烟粉虱与TYLCCNV共侵染可诱导植株内产生较单独取食时更少的多酚类防御物质,以提高其在宿主植物上的适应性。     

内生枯草芽胞杆菌Itb57对烟草疫霉的抑制及生理生化影响

为明确内生枯草芽胞杆菌Itb57菌株抑制烟草疫霉生长的机理,分别采用电镜扫描法、生化测定法和电导法研究了其对烟草疫霉菌丝形态及生理生化指标的影响。结果显示,经Itb57菌株发酵滤液处理的疫霉菌丝畸形膨大、分枝短粗,孢子囊的形成及萌发受到显著抑制,当发酵滤液浓度为50%时,抑制率分别为79.69%和70.85%;且与对照相比,处理组疫霉菌细胞壁主要成分碱溶性、碱不溶性和水溶性的β-1,3-葡聚糖含量分别降低了51.18%、42.56%和39.42%,β-1,3-葡聚糖合成酶活性降低了72.45%,DNA含量降低了14.64%;线粒体复合酶活性显著低于对照,其中复合酶II活性降低了79.25%,菌丝处理液电导率升高。表明枯草芽胞杆菌Itb57菌株发酵滤液能减少疫霉细胞壁主要成分β-1,3-葡聚糖含量,破坏细胞膜的完整性,导致菌丝生长受阻或产生畸形,对烟草黑胫病的控制具有潜在的生防价值。     

高效氯氰菊酯亚致死浓度对桃小食心虫成虫体内解毒酶活性的影响

为研究高效氯氰菊酯亚致死浓度对桃小食心虫Carposina sasakii Matsumura雌、雄蛾体内解毒酶活性的影响,采用药膜法,以LC10、LC20和LC40浓度的高效氯氰菊酯处理桃小食心虫成虫24 h,分别测定药剂压力解除后0、6、12和24 h桃小食心虫雌、雄蛾体内细胞色素P450单加氧酶7-乙氧基香豆素-O-脱乙基酶（ECOD）、羧酸酯酶（CarE）和谷胱甘肽S-转移酶（GSTs）的活性,并分析其动态变化。结果表明:与对照相比,高效氯氰菊酯LC10浓度处理能抑制桃小食心虫成虫的ECOD比活力,且随处理浓度增加,高效氯氰菊酯对ECOD活性的诱导作用逐渐体现,LC40浓度处理组ECOD活性显著高于对照;此外,LC20和LC40浓度处理组可诱导桃小食心虫雄蛾CarE和GSTs比活力的增加,但对于雌蛾则结果相反。当药剂压力解除后,随着处理时间的延长,成虫体内ECOD、CarE和GSTs活性总体表现为先增加后降低,而药剂浓度越高对其解毒酶的诱导或抑制作用越显著,且存在一定性别差异。研究显示,从短期响应来看,桃小食心虫雌、雄蛾体内的3种解毒酶在高效氯氰菊酯亚致死浓度胁迫后均表现较为活跃,酶活性呈现出先增加后降低的动态变化。由于雌、雄蛾个体的生理机能以及对药剂敏感性的差异,这种生理响应机制存在一定的剂量和性别差异。     

Artificial infection of Vaccinium vitis-idaea L. and defence responses to Exobasidium species

An inoculation method for Exobasidium splendidum and Exobasidium vaccinii was developed on the dwarf shrub Vaccinium vitis-idaea. Using inoculated ramets, we investigated whether there are differences between V. vitis-idaea populations in the susceptibility to Exobasidium infections and whether the defence reaction of V. vitis-idaea is visible at a molecular level. Sixteen V. vitis-idaea clones from four populations were propagated in tissue cultures and the ramets were inoculated with E. splendidum or E. vaccinii fungi. The expression of three flavonoid biosynthetic genes (chalcone synthase, dihydroflavonol 4-reductase and anthocyanidin synthase) and the accumulation of flavonoids and hydroxycinnamic acids were determined in response to E. splendidum infection. A pathogenesis-related (PR 4) gene was isolated and its expression was studied in host ramet leaves. To our knowledge, this was the first successful artificial infection reported with E. splendidum. Disease frequencies of the inoculated ramets were between 32% and 47% for E. splendidum and 33% for E. vaccinii, but below 10% in uninoculated control ramets. There were no differences in disease frequencies between V. vitis-idaea populations. Both symptomatic leaves and healthy leaves of diseased ramets showed activation of flavonoid biosynthesis at the gene level, whereas expression of PR 4 was observed only in symptomatic leaves. The increase of flavonoid biosynthesis in healthy leaves of diseased ramets may represent a general response to stress or a role in defence against the pathogen E. splendidium. Ability of V. vitis-idaea to defend chemically against Exobasidium fungi and the heterogeneity of genotypes, age, size, and growth rates in host plant populations might be reasons for the low infection incidence of Exobasidia in nature.     

吡虫啉亚致死剂量对日本通草蛉实验种群生命表的影响

为评价吡虫啉对日本通草蛉Chrysoperla nipponensis(Okamoto)实验种群的亚致死效应,采用浸渍法处理日本通草蛉卵,测定了吡虫啉亚致死剂量LC_(10)、LC_(20)、LC_(30)对日本通草蛉生长发育、繁殖力和生命表参数的影响。结果表明,经吡虫啉亚致死剂量LC_(10)、LC_(20)、LC_(30)处理后,日本通草蛉幼虫发育历期和蛹期分别延长0.86~2.04 d和1.00~2.37 d,平均单头蛹重降低1.16~1.56 mg,羽化率降低6.13%~13.08%;雌虫产卵前期延长0.19~0.51 d,每雌平均产卵量减少17.75~51.64粒,寿命减少1.75~2.84 d;内禀增长力(r_m)、净繁殖率(R_0)和周限增长率(λ)均显著降低,平均世代历期(T)和种群加倍时间(D_t)均显著延长。研究表明,吡虫啉亚致死剂量对日本通草蛉种群的增长具有抑制作用。     

六种植物精油对褐飞虱和稻虱缨小蜂行为的影响

为研发基于植物精油的褐飞虱Nilaparvata lugens驱避剂和稻虱缨小蜂Anagrus nilaparvatae引诱剂,利用嗅觉仪测试了6种植物精油在不同浓度下对褐飞虱和稻虱缨小蜂的行为反应。结果表明,6种植物精油对褐飞虱和稻虱缨小蜂行为的影响具有种类差异性,不同植物精油对昆虫的行为影响有较大差异。此外,褐飞虱和稻虱缨小蜂对植物精油的嗅觉反应还与精油浓度有关,精油浓度范围在100～5 000 mg/L时,山楂油、白木香油、金银花油、肉桂油和鱼腥草油对褐飞虱雌成虫表现出明显的趋避效果,其中山楂油和金银花油的驱避效果最好,二者在100、500、1 000和5 000 mg/L这4个浓度下均能显著驱避褐飞虱。0.1 mg/L和1 mg/L浓度的山楂油、0.1 mg/L浓度的白木香油和1 mg/L浓度的丁香花蕾油对稻虱缨小蜂雌成虫有较强的吸引力。较高浓度10 mg/L的鱼腥草油对稻虱缨小蜂有明显的驱避性。表明植物精油可以明显地影响褐飞虱和稻虱缨小蜂的行为,可作为行为调控物在"推-拉"策略中防控褐飞虱。     

Resistance selection and biochemical characterization of spinosad resistance in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

A Helicoverpa armigera population was collected from Shandong province, China. After 15 generations of selection in the laboratory, the H. armigera strain developed more than 20-fold resistance to spinosad. At LD₅₀ level, no significant cross-resistance was found between spinosad and chlorpyrifos, methomyl, avermectin and chlorfenapyr except for fenvalerate with a low cross-resistance of 2.4-fold. However, LD₉₉ values of fenvalerate against the parental and resistant strains were not different significantly. After inhibitors were used, spinosad resistance could be partially suppressed by piperonylbutoxide (PBO) and triphenylphosphate (TPP), but not by diethylmaleate (DEM). Activities of p-nitroanisole O-demethylase (ODM) developed to 8.26-fold compared with the parental strain, but no obvious changes were found in activities of carboxyl esterase (CarE) and glutathione-S-transferase (GST). The results indicated that resistance to spinosad in the cotton bollworm might be associated with an increase in cytochrome P450 monooxygenase.     

以豌豆修尾蚜为猎物的大草蛉两性生命表和捕食率

为明确大草蛉Chrysopa pallens (Rambur)的发育、存活、繁殖和捕食能力,利用年龄-龄期两性生命表方法,在25 ℃、光周期L 15:D 9条件下研究了以豌豆修尾蚜Megoura japonica (Matsumura)为猎物的大草蛉的生命表及其捕食率。结果表明:大草蛉雌成虫个体的繁殖力为683.6±55.99粒,种群的内禀增长率、周限增长率、净增殖率和平均世代周期分别为0.1247/d、1.133/d、333.64粒/个体和46.5 d;大草蛉幼虫、雌成虫和雄成虫个体的平均捕食率分别为145.25、1 192.47和669.08头。若考虑存活率,个体的净捕食率为1 026.26头;从猎物种群到捕食者后代的转化率为3.08;种群个体的稳定捕食率和周限捕食率分别为5.49和6.22头。表明以豌豆修尾蚜为猎物时,大草蛉表现出较好的个体发育、种群增长和捕食特性,豌豆修尾蚜可作为大草蛉人工繁殖选择的猎物之一。     

Biochemical and molecular diagnosis of insecticide resistance conferred by esterase, MACE, kdr and super-kdr based mechanisms in Italian strains of the peach potato aphid, Myzus persicae (Sulzer)

In this paper we analysed the basis of insecticide resistance in 59 Italian strains of the peach potato aphid Myzus persicae using both molecular and biochemical assays. Our data as a whole clearly indicate that most M. persicae strains (76.3%) have high or extremely high production of an esterase enzyme which sequester and detoxify insecticides with esteric group. Kdr genotypes conferring resistance towards pyrethoids are present in 57.7% of the analysed populations. Moreover, 26.5% of the kdr positive strains possess also the M918T mutation conferring super-kdr phenotype. Strains with modified AChE (MACE) are not so numerous (27.1%), although they can be found almost everywhere in Italy. Considering all the strains analysed, both MACE and kdr phenotypes are associated with high levels of esterase activity. In Central–Southern regions, kdr and MACE resistance mechanisms resulted in linkage disequilibrium. Bioassays performed in order to evaluate the efficacy of a pyrethroid insecticide against a strain possessing a F979S mutation within its para-type sodium channel gene suggests that this amino acid substitution could affect the sodium channel responsivity to pyrethroids.     

Pythium rot of chingensai ( Brassica campestris L. chinensis group) caused by Pythium ultimum var. ultimum and Pythium aphanidermatum

Severe rot was found at the base of leaves and stems of chingensai (Brassica campestris L. chinensis group) in Okayama Prefecture in 2000. The causal fungi were morphologically identified as Pythium ultimum Trow var. ultimum and P. aphanidermatum (Edson) Fitzpatrick. This is the first report of rot caused by Pythium species on chingensai. We named this disease Pythium rot of chingensai.     

黄带犀猎蝽对斜纹夜蛾3、4龄幼虫的捕食功能反应

为明确黄带犀猎蝽Sycanus croceovittatus对斜纹夜蛾Spodoptera litura的控害潜能,在实验室条件下对黄带犀猎蝽3~5龄若虫和雌雄成虫对斜纹夜蛾3、4龄幼虫的捕食功能反应、搜寻效应和自身密度干扰反应进行研究。结果表明,黄带犀猎蝽对斜纹夜蛾幼虫的捕食功能反应均符合Hol-ling II型和Holling III型2种模型;在拟合Holling II模型中,黄带犀猎蝽3~5龄若虫和雌雄成虫对斜纹夜蛾3龄幼虫的日均最大捕食量分别为2.78、4.17、4.35、12.50和8.33头,控害效能分别为5.53、6.54、8.78、14.75和10.42;对斜纹夜蛾4龄幼虫的日均最大捕食量分别为2.56、2.86、3.85、3.57和4.35头,控害效能分别为2.23、3.94、4.54、11.32和5.70,其中雌成虫的控害效能最大。在拟合Hol-ling III模型中,对斜纹夜蛾3龄幼虫的日均最大捕食量分别为2.75、4.10、4.32、10.76和7.41头,最佳寻找密度分别为1.19、2.03、1.73、5.28和3.88头/盒;对斜纹夜蛾4龄幼虫的日均最大捕食量分别为2.38、2.78、3.55、3.58和4.00头,最佳寻找密度分别为1.83、1.50、2.06、0.95和2.10头/盒。搜寻效应与害虫密度呈负相关;种内干扰效应与天敌密度呈正相关。表明黄带犀猎蝽成虫对斜纹夜蛾3、4龄幼虫的捕食能力优于黄带犀猎蝽若虫。