猪巴氏杆菌病疫苗生产菌种保存期试验

对３个不同冻干年代２～８℃保存１０年以上的猪源多杀性巴氏杆菌Ｃ４４－１、Ｃ４４－８、６７９－２３０冻干菌种按《中华人民共和国兽用生物制品规程》有关规定进行了冻干菌种存活情况检查和全面系统鉴定，结果表明此３株冻干菌种在２～８℃保存１０年，其菌体形态、菌落特征、生化特性、血清学特性、毒力（安全性）、免疫原性均符合《中华人民共和国兽用生物制品规程》规定标准。     

牛巴氏杆菌病灭活疫苗生产菌种的保存期试验

对在２～８℃保存１０年以上，不同时期制备的牛源多杀性巴工杆菌生产用冻干菌种Ｃ４５－２、Ｃ４６－２、Ｃ４７－２各３批经培养繁殖，对菌种特性做了全面系统鉴定，结果各批菌种的各项特性均符合《中华人民共和国兽用生物制品规程》规定标准。     

禽多杀性巴氏杆菌病疫苗生产用菌种保存期试验

对三个不同冻干年代２￣８℃保存１０年以上的Ｃ４８－１、Ｃ４８－２以及保存２二个不同年龄１５０２冻干菌种按《中华人民共和国兽用生物制品规程》有关规定进行了力种存活情况检查和全面系统鉴定,结果表明Ｃ４８－１、４８－２、１５０２冻干菌种在２￣８℃保存１０年以上,其形态、菌落特征、生化特性、血清学特性、毒力、免疫原性均符合《中华人民共和国兽用生物制品规程》规定标准。     

禽巴氏杆菌病B26—T1200弱毒疫苗保存期试验

禽巴氏杆菌病Ｂ２６－Ｔ１２００弱毒疫苗在４－８℃温度环境中保存一年的１０批苗，菌存率为７６．７－９３．３％，平均菌存率为８６．４％；按瓶签头份稀释后免疫鸡，１０批苗的保护率７５－１００％，总保护率为８７．５％（３７／４０）。     

兔出血症-巴氏杆菌病蜂胶二联灭活苗保存期试验

研制兔出血症-巴氏杆菌病蜂胶二联灭活苗2批,分别在4-8℃和室温条件下保存6个月、12个月后进行免疫保护试验。结果显示：蜂胶二联苗在4～8℃和室温条件下保存6个月、12个月,对兔出血症的保护率均为100％;4～8℃保存6个月对巴氏杆菌病的保护率为100％,保存12个月对巴氏杆菌病的保护率为90％;室温保存6个月对巴氏杆菌病的保护率80％,保存12个月对巴氏杆菌病的保护率为70％。据此可以确定蜂胶二联苗的保存期为4～8℃一年或室温半年。     

8301多糖保存期及其对小鼠抗御禽源巴氏杆菌感染作用的测定

用１％８３０１多糖悬浮液给小白鼠注５次,每次０．２ｍＬ,停药１５ｄ后与１０只对照小白鼠同时以一个最小致死量的禽源巴氏杆菌攻击,测得８３０１多糖原粉及其配制的悬浮液,其保存期分别在８ａ和２０个月以上;对小白鼠的保护率在１／９￣５／１０（１１￣５０％）之间,对照小白鼠则全部死亡。     

双歧杆菌在培养液中的保存期试验

双歧杆菌对人和动物都是有益细菌 ,以双歧杆菌制成的微生态制剂也倍受关注 ,由于双歧杆菌是严格的厌氧菌 ,在制品中存活时间很短。本试验以两株双歧杆菌在液体培养基中培养后 ,置7℃冰箱中保存 ,一株到第 4天 ,另一株到第 8天时 ,其活菌数减少 99% ,至 2 0天时无活菌存在。在加有保护剂的菌液中 ,保存至 2 4天尚有活菌存在。尽管如此 ,双歧杆菌制剂的保存期仍然很短的     

牛,羊,猪产气荚膜杆菌和多杀性巴氏杆菌混合感染

牛、羊、猪产气荚膜杆菌和多杀性巴氏杆菌混合感染卢中华，毛春生，王自振，曹定盈（河南农业大学郑州４５０００２）侯安祖，刘香仁，陈洪科（河南省兽医防治站）１９８９年以来，在豫东、豫西南和安徽西部地区散发一种以牛、羊、猪突然死亡、全身实质器官及消化道急性出...     

牛猪巴氏杆菌病的防治

2000年9月,龙街乡某村的水牛和猪暴发了一起致死性流行性疫病,经流行病学调查、临床症状、病理剖检及镜检,诊断为牛、猪的巴氏杆菌病.     

选择牛巴氏杆菌病灭活疫苗菌种的参考依据

选择牛巴氏杆菌病灭活疫苗菌种的参考依据康凯钱心元中国兽药监察所北京１０００８１菌种是制备生物制品的基础，其质量优劣直接关系到制品质量，选择免疫原性良好的生产用菌种是提高产品质量的重要保证。自５０年代末我国研究成功牛巴氏杆菌灭活疫苗以来，我所长期保存着...     

Detection of neutralizing antibodies against Pasteurella multocida toxin in swine with atrophic rhinitis

Blood sera of 1171 pigs, not vaccinated against PAR, were tested for antibodies against P. multocida toxin in a neutralization test with EBL cell cultures. 277 sera were from 18 herds with clinical PAR; 104 of them (37.5%) had neutralizing antibody titres from 1:2 to 1:1024. No antitoxin was detected in the sera of 866 pigs in 25 PAR nonsuspicious herds. In one nonsuspicious herd, however, 11 of 28 sera were neutralizing in the 1:2 or 1:4 dilution. 30 sows had been vaccinated with inactivated toxigenic P. multocida and/or with the P. multocida toxoid. 21 of these sows had neutralizing sera with titres between 1:8 and 1:4096. The neutralization test presented can be applied for herd diagnosis of PAR and for demonstration of vaccination effects.     

Incubation of Pasteurella haemolytica and Pasteurella multocida lipopolysaccharide with sheep lung surfactant

Lipopolysaccharides (LPS) were extracted from a serotype of each of 2 species of Pasteurella isolated from sheep with respiratory tract infections. Lipopolysaccharides from P haemolytica 82-25 (serotype 1A) or P multocida P-1573 (serotype 12) were mixed with sheep lung surfactant and were incubated for 6 hours at 37 C. After incubation, LPS-surfactant mixtures were centrifuged overnight in sucrose density gradients, and fractions were analyzed. Binding occurred between LPS and surfactant vesicles resulting in a stable complex with densities greater than those with the surfactant alone. The surfactant alone had a density of 1.052 to 1.060 g/ml. Diffuse bands of surfactant had a density of 1.075 to 1.092 when incubated with P haemolytica LPS and a density of 1.069 to 1.105 when incubated with P multocida LPS.     

Experimental infection of sheep with Mycoplasma ovipneumoniae and Pasteurella haemolytica

A group of Caesarian-derived, colostrum-deprived lambs was inoculated intranasally and intratracheally with a virulent Mycoplasma ovipneumoniae isolate selected from ovine mammary studies and propagated in an ovine mammary gland. Other groups of lambs were inoculated with M. ovipneumoniae in combination with Pasteurella haemolytica type Al or P. haemolytica alone. The M. ovipneumoniae isolate alone did not induce any specific pneumonic lesions in the lambs and when combined with P. haemolytica type Al did not increase the severity of the P. haemolytica-type lesions. Fifty percent of lambs inoculated with P. haemolytica developed a purulent and exudative bronchopneumonia with pleurisy and high titres of P. haemolytica were recovered from these lesions.     

猪瘟淋脾毒耐热保护剂活疫苗保存期的测定及免疫保护试验

对添加耐热保护剂的猪瘟淋脾毒活疫苗进行保存期的测定和保存后的免疫保护试验.结果表明,研制的猪瘟淋脾毒耐热保护剂活疫苗在2～8 ℃条件下保存24个月后免疫猪,能100%抵抗猪瘟石门系强毒的攻击.     

Effect of Pasteurella multocida and Haemophilus pleuropneumoniae toxins on swine alveolar macrophages

Supernatants were obtained from 18 hr. broth cultures of Pasteurella multocida strains D82 and D62 (serotype D, toxigenic), Kobe 6 (type D, non-toxigenic), A50 and X73 (type A, non-toxigenic), Haemophilus pleuropneumoniae serotypes 1, 5 and 6, Haemophilus sp. taxon "minor group" (2 strains) and an avirulent serotype 1 strain of H. pleuropneumoniae. The supernates were filtered, pH-neutralized and tested for cytotoxicity after incubation for 18 hours in the presence of swine alveolar macrophage monolayers. Supernatants from H. pleuropneumoniae serotypes 1, 5 and 6 were cytocidal.     

Effect of infections with swine fever virus on immune functions. III. Antibody response to lipopolysaccharide and sheep red blood cells

In order to determine the effect of infections with low-virulent swine fever virus (SFV) on antibody responses, pigs were administered lipopolysaccharide (LPS) or sheep red blood cells (SRBC), 2 days after infection. Infected pigs showed an enhanced primary response to LPS late during infection. The secondary response to LPS seemed to be unaffected. Both the primary and secondary antibody response to SRBC appeared to be enhanced rather than depressed in infected pigs. These in vivo findings suggest that pigs infected with low-virulent SFV do not develop a depression of B lymphocyte function.