Genetic variation for dry matter and nitrogen accumulation and translocation in two-rowed spring barley: I. Dry matter translocation

A 4-year field study was carried out to determine dry matter and nitrogen accumulation until anthesis and at grain filling period and dry matter translocation and utilization in grain filling of barley. Twenty two-rowed spring barley (Hordeum vulgare ssp. distichum L.) cultivars originated from different countries (Yugoslavia, Germany, Australia, the Czeck Republic, Netherlands, France and USA) were grown during 1995–1998 on a non-calcareous chernozem soil near Novi Sad (45° 20′N, 15° 51′E, 86 m asl). Dry matter and nitrogen accumulation depended on the cultivar and year. In a year with favorable weather conditions, 58% of dry matter was accumulated during pre-anthesis, while in a year with less favorable weather the amount was 48%. In the favorable year 91% and in unfavorable year 65% of nitrogen was accumulated until anthesis. The results indicated that the greater amount of dry matter and nitrogen accumulated before anthesis. Dry matter translocation efficiency depended on the cultivar and ranged from 3 to 16.4%, while the contribution of pre-anthesis assimilates to kernel varied from 4 to 24.2%. Cultivars that have been developed for the growing conditions of the area where the experimental site was located, i.e. adapted ones, did not use pre-anthesis dry matter for grain filling. High positive correlations (P<0.01) were found between biomass at anthesis and biological yield, dry matter translocation efficiency, contribution of translocated dry matter to grain yield, and total plant nitrogen at maturity. Accumulated nitrogen at anthesis was positively correlated (P<0.01) with growing degree–days until anthesis, dry matter at anthesis and dry matter translocation parameters. Heritability for the investigated characters was rather high, over 0.60.     

The effect of lupins as compared with peas and oats on the yield of the subsequent winter barley crop

New high yielding early maturing cultivars of lupins have been introduced in north-west Europe as grain protein crops in crop rotations. This paper reports on a comparative study of lupins with peas and oats, and of their effect on yield of subsequent winter barley crops. These crops were given five levels of N under irrigated and non-irrigated conditions on sand and loam. Under rain fed conditions the grain yield of pea, oat and lupin varied between 24–36, 34–53 and 18–37 hkg DM ha⁻¹, respectively. Supplemental irrigation raised grain yield of oat to 50–60 hkg DM ha⁻¹, while grain yield in pea was not affected and grain yield in lupin in most cases decreased due to gray mould attack and excessive vegetative growth in the indeterminate lupin variety. Under rain fed conditions, the grain nitrogen content of pea, oat and lupin varied between 137–172, 61–80 and 189–226 kg N ha⁻¹, respectively, and was significantly higher in lupin as compared with pea. On sandy soil, similar low-root densities were found for pea, oat and lupin below 30 cm depth. On sand, at final harvest the residual soil-N of lupin and pea, as measured in a subsequent winter barley crop not supplied with N fertilizer, was 15 and 8–10 kg N ha⁻¹ higher than in winter barley following oat, respectively. The nature of the probably more N-root residues of lupin is discussed. On loam, the residual N of lupin and pea was similar, 18–27 kg N ha⁻¹. On sand, under rain fed conditions preceding lupin and pea as compared with oat, increased the barley grain yield at zero N-application 77 and 49%, respectively; the effect of lupin was significantly higher than that of pea until the highest N-level 120 kg N-application ha⁻¹. On loam under rain fed conditions preceding lupin and pea increased the barley grain yield at zero N-application by 36 and 62%, respectively, as compared with oat; at N-application>60 kg N ha⁻¹ the grain yield was similar after all three crops. For both soil types the same level of effect was found under irrigated conditions. Conclusions: Supplemental irrigation might result in lower grain yield in lupin due to gray mould attack and excessive growth if indeterminate lupin varieties are used. Grain nitrogen yield of lupin is significantly higher than that of pea. On sand, the effect of lupin on the subsequent winter barley grain yield is significantly higher than that of pea, probably due to greater N-root nitrogen residues. On loam, lupin and pea have similar effects on the subsequent winter barley crop.     

Effect of preceding crop combination and N fertilization on yield of six oil-seed rape cultivars (Brassica napus L.)

Information about the effect of the cropping history on the seed yield of oil-seed rape is extremely scarce. In 1992/93 and 1994/95, the effects of different preceding crop combinations (winter barley and winter wheat as preceding crops, oil-seed rape and wheat as pre-preceding crops) on the yield of six double low oil-seed rape cultivars were examined in a field trial at Hohenschulen Experimental Farm, north-west Germany. In addition, eight nitrogen treatments (different amounts and distribution patterns) were tested for their potential to reduce negative effects of the preceding crops. Following the cropping sequence of oil-seed rape then wheat, oil-seed rape yielded only 3.12 t ha⁻¹; after oil-seed rape then barley, the yield was 3.43 t ha⁻¹ compared with 3.77 t ha⁻¹ following wheat then barley and 3.71 t ha⁻¹ following wheat then wheat. The number of seeds per m² showed a similar pattern, whereas the thousand-seed weight partly compensated for the reduced seed number. It was highest if oil-seed rape was grown 2 years previously. The cultivars differed significantly in their yield potential. Express (3.79 t ha⁻¹) yielded 0.6 t ha⁻¹ more than Falcon (3.18 t ha⁻¹). Increasing amounts of fertilizer-N (80–200 kg N ha⁻¹) increased the seed yield from 3.21 t ha⁻¹ to 3.84 t ha⁻¹. Changes in the distribution pattern within one fertilizer amount had no effect on seed yield. In addition, no interactions between preceding crop combination and the different cultivars or N treatments occurred. It is concluded that crop management cannot totally eliminate the negative effects of an unfavourable cropping history on the seed yield of oil-seed rape.     

施氮量对小麦花后氮素分配及氮素利用的影响

为研究黄淮海麦区化肥投入不断增加,而产量却徘徊不前的问题,以当地主栽品种矮抗58和周麦22为材料,采用4个施氮水平（0、120、240、360kg/hm ²）和品种的二因素分析方法研究了施氮量对小麦植株地上部各器官氮素分配及氮素利用的影响。结果表明：开花期至成熟期小麦植株地上部各营养器官氮含量和氮素积累量均下降。施氮量对开花期和成熟期小麦植株地上部各器官氮含量的影响均达显著水平,增加氮肥能显著促进小麦营养器官氮素向子粒转运和花后氮同化。开花期周麦22叶片氮素转运量优于矮抗58。矮抗58和周麦22花后氮同化量均以施氮量360kg/hm ²最高,花前氮素积累转运量对子粒贡献率达60.25%~97.55%,子粒氮收获指数为59.82%~79.48%,随施氮量的增加而呈下降趋势。施氮量120kg/hm ²处理的氮素养分利用效率、农学利用效率及生产效率均最高。增施氮肥对小麦子粒产量有显著促进影响,矮抗58在施氮量为360kg/hm ²时有最大子粒产量,周麦22在施氮量为240kg/hm ²时有最大子粒产量。推荐矮抗58和周麦22在黄淮海麦区的施氮量为240~360kg/hm ²。     

Wheat productivity in the Mediterranean Ebro Valley: Analyzing the gap between attainable and potential yield with a simulation model

Water deficit is an important constraint for wheat yield generation under Mediterranean environments. However, nitrogen (N) availability could limit yield in a more important way than poor water conditions. The aim of the work was to analyze, using the Ceres-Wheat crop simulation model, to what degree N fertilization constitutes a tool for reducing the gap between attainable and potential yield. Firstly, the model was calibrated and validated under a wide range of N and water conditions for the region of the Ebro Valley (NE Spain). Anthesis and maturity date were adequately predicted by the model. Predictions of yield tended to be quite accurate in general, though under severe water deficits precision was lower. We then assessed the gap between attainable and potential yield considering different N availabilities at sowing taking into account a weather database of 17 years for the location of Agramunt (NE Spain), representative of cereal growing conditions of the Mediterranean Catalonia. Potential yield ranged between 3.5 and 8.1 Mg ha⁻¹. Variations in potential yield were explained by the duration of the period from sowing to anthesis and by the level of incident radiation during the period immediately previous to anthesis. Average attainable yield was 1.8 Mg ha⁻¹ for N availability of 50 kg_N ha⁻¹; but increased to 2.8 Mg ha⁻¹ for higher N availabilities (100–250 kg_N ha⁻¹). In the 25% of the worst years there was no effect of N availability on attainable yield. Increasing N availability beyond 100 kg_N ha⁻¹ generated a gain in yield only in 6% of the years. Variations between years in attainable yields were mainly explained by rainfall during the period from sowing to anthesis, whereas differences in attainable yield between N treatments increased with increases in rainfall. The gap between potential yield and attainable yield was higher in years with higher potential yield. On the other hand, the higher the attainable yield, the lower the gap. Thus, the proportion of the yield gap ascribed to N availability varied depending on the conditions of the growing season. In the high-yielding potential years, the main restriction for growth was water shortage, and fertilizing only slightly reduced the gap. Conversely, in rainy years characterized by low potential yields and mild water stresses, N management may constitute a simple tool for effectively reducing yield gap under rain-fed conditions.     

Nitrogen Fertilizer Effects on the Duration of the Pre-anthesis Period and Spikelet Number per Spike in Barley

The effects of increased yield and grain number per unit area in barley in response to nitrogen application are well known. However, the influence of applied nitrogen on the rates and durations of developmental phases in barley are less well understood. Our objective was to investigate the effect of applied nitrogen on the duration of pre-anthesis development in barley and the number of spikelets per spike in two barley cultivars, Franklin and Schooner, in two studies. We found no effect of nitrogen on the duration of the pre-anthesis period in Schooner, when applied to pots at a rate of 0 or 55 kg N ha^-1, or when applied in the held at 0, 40 or 160 kg N ha^-1. However, this duration was extended in Franklin in the first study by an application of 55 kg N ha^-1. Both plant biomass and grain yield at maturity were increased between 0 and 55 kg N ha^-1, and 0 and 160 kg N ha^-1. Meld increase was largely associated with an increase in the number of tillers per plant.     

施氮水平对啤酒大麦植株氮素吸收与利用及籽粒蛋白质积累和产量的影响

2004—2006年连续两个生长季,以苏啤3和单2两个啤酒大麦品种为材料,探讨施纯氮0、75、150、225和300 kg hm^-2条件下,啤酒大麦氮素积累和转运、氮素利用及籽粒产量和蛋白质积累的特性。在0~225 kg hm^-2施氮量范围内,啤酒大麦花前植株氮素积累量和转运量均随施氮水平的提高呈上升趋势,但施氮量提高至300 kg hm^-2后,提高幅度变小;而花前氮素转运效率及其对籽粒氮的贡献率则均随施氮水平提高呈单峰曲线变化。籽粒谷氨酰胺合成酶和谷-丙转氨酶活性也随着施氮水平的提高而上升,促进蛋白质积累,提高籽粒蛋白质含量,而当施氮量低于197 kg hm^-2时籽粒蛋白质含量才低于12%,符合啤酒大麦酿造要求。经回归分析,在施氮量为241 kg hm^-2时产量最高。此外,氮肥回收效率以225 kg hm^-2施氮处理为最高,氮素生理利用效率和氮收获指数随施氮量增加而显著降低。综合考虑各项指标,建议在类似本试验条件的啤酒大麦生产区,施氮量以150~197 kg hm^-2为宜。     

N2 fixation and N supply in organic pea (Pisum sativum L.) cropping systems as affected by weeds and peaweevil (Sitona lineatus L.)

Grain legumes, especially peas, could play a key role in organic cropping systems. They could provide nitrogen (N) to the system via N₂ fixation and produce grain rich in protein while improving soil N for the succeeding crop. Thus, maximising N₂ fixation and optimising grain N production together with N contribution to soil is a challenging issue for organic pea crops. However, pest, disease and weed infestation are less easy to control in organic systems than in conventional systems. Therefore, the effects of weed infestation and pea weevil (Sitona lineatus L.) attacks on N nutrition and N₂ fixation of organic pea crops were examined by on-farm monitoring over two years. The magnitude of the net contribution of the crops to the soil N balance in relation to their productivity was also assessed. In many situations, weed infestation together with pea weevil damage severely limited the nitrogen nutrition and grain yield. Percentage of N derived from fixation (%Ndfa) increased with weed biomass because weeds appeared more competitive than peas for soil N. But %Ndfa decreased with pea weevil leaf damage score. The interaction between these two biotic factors affected N yields and the net contribution of the crops to soil N. This latter ranged from −133 kg N ha⁻¹ to 69 kg N ha⁻¹ depending on %Ndfa and nitrogen harvest index (NHI). Optimising both grain N and net balance would require a reduction in root nodule damage by weevil larvae in order to maximise %Ndfa and a reduction in the NHI through the choice of cultivar and/or suitable crop management.     

不同氮收获指数水稻基因型的氮代谢特征

采用土培盆栽试验,以3个氮收获指数(NHI)有显著差异的水稻基因型4434(低NHI)、滇瑞302(中NHI)和余赤23(高NHI)为材料,研究了灌浆期叶片、穗颈和籽粒的氮代谢特点及与NHI的关系。结果表明,各基因型的籽粒产量、收获指数和籽粒氮积累量与NHI的变化一致,均以余赤231最大。花后植株氮素转运量表现为4434<滇瑞302<余赤231,基因型间差异极显著,而氮素转运率和转运氮的贡献率差异较小。成熟期水稻茎叶和籽粒的全氮含量、蛋白氮和非蛋白氮含量均表现为4434<滇瑞302<余赤231,全氮含量和蛋白氮含量存在显著差异,而非蛋白氮无显著差异;余赤231茎叶蛋白氮积累量显著低于4434和滇瑞302,而籽粒蛋白氮积累量显著升高,是高NHI水稻氮积累的主要特征。余赤231灌浆期叶片和籽粒谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)活性显著高于4434和滇瑞302,有利于叶片游离氨基酸合成及外运,使得穗颈节伤流强度和游离氨基酸含量升高,为籽粒氮素积累提供了物质基础;同时,较高的籽粒GS和GOGAT活性促进了籽粒蛋白质合成,提高了NHI。逐步回归表明,灌浆期较高的穗颈伤流游离氨基酸含量是高NHI水稻氮代谢的主要生理特征,与较高的花后氮转运量和籽粒蛋白氮积累量可共同作为水稻氮素高效管理和遗传改良的可靠指标。     

Effect of varied N rates and N timings on yield, N uptake and fertilizer N use efficiency of a six-row and a two-row winter barley

The effects of N rates and N timings on yield formation, N uptake at five growth stages and fertilizer N use efficiency of six-row and two-row winter barley were evaluated in field trials conducted from 1990/91 to 1992/93 at the TU Munich's research station Roggenstein.

On average over 3 years the six-row cultivar yielded most at a total rate of 110 kg ha⁻¹ N including an early application of 40 kg ha⁻¹ N up to EC 30 (Zadoks scale). The two-row cultivar achieved maximum yield at a total rate of 140 kg ha⁻¹ N including early applications of 70 kg ha⁻¹ N up to EC 30. The highest yielding N-treatments of six-row barley regularly took up less nitrogen at EC 32 (95 kg ha⁻¹ N on average) than the non-optimally fertilized treatments, whereas full exploitation of the yield potential of two-row barley was associated with higher rates of N-uptake at EC 32 (113 kg ha⁻¹ N on average).

Lodging did not occur in the trials conducted in 1991 and 1992 and no difference was detected between the two cultivars in fertilizer N use efficiency. With six-row barley the N treatment giving maximum yield also led to an optimum fertilizer N use efficiency. Full exploitation of the two-row barley yield potential was associated with suboptimal fertilizer N use efficiencies.     

Nitrogen Accumulation and Translocation for Winter Wheat under Different Irrigation Regimes

The translocation of pre‐anthesis nitrogen to the grain is an important source for winter wheat. The relation between the nitrogen translocation and irrigation regime was studied in the field under a rain‐proof trough shelter. Nitrogen (N) translocation amount, N translocation efficiency decreased with a decline in irrigation amount or by excessive irrigation. Compared with different organs, the leaf and stem had higher N translocation amounts, and contributions to grain for both cultivars – Jinan 17 and Lumai 21, indicating that stem also is a major N source for grain development. The contribution of pre‐anthesis total above ground N to grain N ranged from 57 to 76 %, indicating the importance of pre‐anthesis storage of N for achieving high grain N concentrations. Grain nitrogen and yield (kg ha^?1) were positively and significantly correlated with the N translocation amounts and contributions, respectively, suggesting that the sink strength may be involved in the translocation of N from a vegetative organ to the grain. N harvest index (NHI) was significantly correlated with N translocation efficiency, suggesting that the latter is a prerequisite for increasing grain N and improving grain quality. The experiment showed that N translocation status is enhanced by better irrigation practices, but limited by severely deficient or excessive irrigation.     

Importance of P uptake efficiency versus P utilization for wheat yield in acid and calcareous soils in Mexico

There are large agricultural areas in the world where wheat yields are limited by low phosphorus (P) availability. Breeding for P uptake and P utilization efficiency may reduce this problem. This study was conducted to determine the contribution of P uptake and utilization efficiency to grain yield of selected spring wheat genotypes in different environments. Thirty-eight semidwarf spring bread wheat (Triticum aestivum) genotypes were grown in two experiments in Mexico, each on an acid Andisol under rainfed conditions and on a calcareous Aridisol with irrigation, without (−P) and with 35 kg P per ha fertilized (+P). Without P fertilization, grain yield ranged from 0.8 to 4.6 t ha⁻¹ in the acid soil and from 2.4 to 5.2 t ha⁻¹ in the calcareous soil. With P fertilization, this range was even larger. Under conditions of P deficiency, i.e. in the acid soil at −P and +P (high P adsorption) and calcareous soil at −P (P-depleted soil), P uptake explained 71–100% of the variation in grain yield, and was highly correlated with grain yield (r=0.79–0.95). In contrast, at +P in the calcareous soil, P utilization efficiency explained 60–63% of the variation in grain yield. Here, low grain P concentration was related to high grain yield (r=−0.40 to −0.59). In the calcareous soil, the harvest index was correlated with grain yield, irrespective of the P level. In the acid soil, post-anthesis P accumulation was important. It was positively correlated with grain yield, whereas in the calcareous soil, no post-anthesis-P accumulation occurred. Here, grain P accumulation at maturity was completely determined by translocation of pre-anthesis shoot P. We conclude that the combination of improved P uptake and P utilization efficiency in the same genotypes requires selection under both high and low-P conditions.     

旱稻297非结构性碳水化合物的生产与产量构成因子的关系

以旱稻297为试验材料,比较了在不施氮肥和150 kg hm-2的施氮量下旱稻297非结构性碳水化合物的生产能力、运转特点及其与产量构成因子的关系,分析了旱稻297氮肥投入与碳水化合物生产和产量形成间的关系。试验结果表明,开花前储藏的非结构性碳水化合物对产量的贡献率为32%~54%,施氮降低了开花前非结构性碳水化合物对产量的贡献率,相对而言开花后光合产物对产量的贡献率略有提高;开花前非结构性碳水化合物的转移效率为48%~65%,施氮后转移效率略有降低;总体而言,施氮降低了开花前后分配给单个籽粒的非结构性碳水化合物的数量,导致千粒重降低;在一定的范围内,随着开花期叶片中可溶性糖浓度的提高,结实率显著提高,但是随着穗中淀粉浓度的提高,结实率显著降低。因此,施氮后非结构性碳水化合物积累不足和转移效率降低同时限制了千粒重和结实率的提高,而叶片中可溶性糖浓度偏低和穗中淀粉浓度偏高限制了结实率的提高,是限制产量提高的主要原因。此外,旱稻297花后光合产物生产能力较低,是限制产量提高的又一原因。     

CO2浓度升高和施氮对冬小麦花前贮存碳氮转运的影响

为探讨大气CO₂浓度升高对冬小麦花前贮存碳氮转运的影响及氮素营养的调节作用,以小偃22和小偃6号为材料,于2007—2009连续2个生长季,利用开顶式气室进行盆栽试验,对背景CO₂浓度(375 μL L^-1)和高CO₂浓度(2007—2008年度680 μL L^-1, 2008—2009年度750 μL L^-1)条件下不同施氮处理的干物质和氮素在籽粒、花前地上部中的累积以及花后营养器官的转运进行了评价。2007—2008年度设4个施氮水平,分别是0、0.1、0.2和0.3 g kg^-1土; 2008—2009年度设3个施氮水平,分别是0、0.15和0.30 g kg^-1土。结果表明,施氮和CO₂浓度升高促进了干物质和氮素在籽粒和花前营养器官的积累,增加了花前营养器官和地上部贮存干物质和氮素向籽粒的转运量,适量施氮提高了CO₂浓度升高对花前营养器官干物质和氮素累积以及花后向籽粒转运的正向效应。与背景CO₂浓度相比,高CO₂浓度提高了花前营养器官和地上部干物质对籽粒产量的贡献率和转运率,但CO₂浓度升高对花前氮素的贡献率和转运率的影响因年份和品种而异。CO₂浓度升高后,2007—2008年度各营养器官和地上部,以及2008—2009年度茎鞘和穗的氮素贡献率和转运率均增加,但2008—2009年度2个品种叶片和地上部氮素贡献率在施氮时均显著降低,小偃22叶片和地上部氮素转运率在各施氮水平下以及小偃6号地上部氮素转运率在0.13 g kg^-1土施氮水平下均明显增加。适量施氮也在大多数情况下增强了CO₂浓度升高对营养器官干物质和氮素的贡献率和转运率的正向效应。说明CO₂浓度升高后小麦产量和氮素积累增加与其促进花前干物质和氮素积累及花后向籽粒的转运密切相关。     

Delaying senescence of wheat with fungicides has interacting effects with cultivar on grain sulphur concentration but not with sulphur yield or nitrogen:sulphur ratios

Winter wheat was grown in three field experiments, each repeated over two or three seasons, to investigate effects of extending flag leaf life by fungicide application on the concentration, kg ha⁻¹ and mg grain⁻¹ of nitrogen (N) and sulphur (S) as well as N:S ratio and sodium dodecyl sulphate (SDS) sedimentation volume. The experiments involved up to six cultivars and different application rates, timings and frequencies of azoxystrobin and epoxiconazole. For every day the duration to 37% green flag leaf area (m) was extended, N yield was increased by 2.58 kg ha⁻¹, N per grain by 0.00957 mg, S yield by 0.186 kg ha⁻¹ and S per grain by 0.000718 mg. The N:S ratio decreased by 0.0135 per day. There was no evidence that these responses varied with cultivar. In contrast, the relationship between flag leaf life and N or S concentration interacted with cultivar. The N and S concentrations of Shamrock, the cultivar that suffered most from brown rust (Puccinia recondita), increased with the extension of flag leaf life whereas the concentrations of N and S in Malacca, a cultivar more susceptible to Septoria tritici, decreased as flag leaf senescence was delayed. This was because the relationships between m and N and S yields were much better conserved over cultivars than those between m and thousand grain weight (TGW) and grain yield ha⁻¹.     

Grain nitrogen content of winter bread wheat (Triticum aestivum L.) as related to crop management and to the previous crop

Low-input management for wheat production (less fungicide, low nitrogen rate) could affect grain protein content. The impact of the previous crop could also be a determining factor for wheat quality. A long-term field experiment located near Toulouse (southwestern France), comparing different rotations and management schemes from 1984 to 1993, was used to assess the effect of N availability and diseases on grain nitrogen concentration (GNC) of wheat. GNC ranged from 1.6 to 2.8%, increasing with the input level in 5 years out of 10, as the result of higher nitrogen levels and crop protection with fungicides. Leaf brown rust, high temperatures and water shortage, which affected dry matter accumulation during grain filling, were responsible for high GNC. GNC was generally correlated with N uptake when nitrogen availability was the main limiting factor and to the nitrogen harvest index (NHI) when foliar diseases or drought limited grain production. N uptake in the absence of N fertiliser ranged from 20 to 200 kg ha⁻¹ depending on the previous crop. GNC was closely related to the nitrogen nutrition index (NNI) at anthesis.     

春小麦不同品质类型氮的吸收、转化利用及与籽粒产量和蛋白质含量的关系

以不同品质类型春小麦为材料,研究产量和蛋白质形成过程中营养体各器官氮的水平动态以及氮的转化和利用效率。结果表明,植株营养体各器官干物质总的积累趋势为开花期开始增加,达高峰后逐渐下降至成熟;氮的含量变化为开花期最高,随籽粒灌浆逐渐下降,成熟时降至最低。籽粒氮含量变化呈“V”型曲线,籽粒氮素积累呈“S”型     

Tillage, cover crops, and nitrogen fertilization effects on soil nitrogen and cotton and sorghum yields

Sustainable soil and crop management practices that reduce soil erosion and nitrogen (N) leaching, conserve soil organic matter, and optimize cotton and sorghum yields still remain a challenge. We examined the influence of three tillage practices (no-till, strip till and chisel till), four cover crops {legume [hairy vetch (Vicia villosa Roth)], nonlegume [rye (Secaele cereale L.)], vetch/rye biculture and winter weeds or no cover crop}, and three N fertilization rates (0, 60–65 and 120–130 kg N ha⁻¹) on soil inorganic N content at the 0–30 cm depth and yields and N uptake of cotton (Gossypium hirsutum L.) and sorghum [Sorghum bicolor (L.) Moench]. A field experiment was conducted on Dothan sandy loam (fine-loamy, siliceous, thermic, Plinthic Paleudults) from 1999 to 2002 in Georgia, USA. Nitrogen supplied by cover crops was greater with vetch and vetch/rye biculture than with rye and weeds. Soil inorganic N at the 0–10 and 10–30 cm depths increased with increasing N rate and were greater with vetch than with rye and weeds in April 2000 and 2002. Inorganic N at 0–10 cm was also greater with vetch than with rye in no-till, greater with vetch/rye than with rye and weeds in strip till, and greater with vetch than with rye and weeds in chisel till. In 2000, cotton lint yield and N uptake were greater in no-till with rye or 60 kg N ha⁻¹ than in other treatments, but biomass (stems + leaves) yield and N uptake were greater with vetch and vetch/rye than with rye or weeds, and greater with 60 and 120 than with 0 kg N ha⁻¹. In 2001, sorghum grain yield, biomass yield, and N uptake were greater in strip till and chisel till than in no-till, and greater in vetch and vetch/rye with or without N than in rye and weeds with 0 or 65 kg N ha⁻¹. In 2002, cotton lint yield and N uptake were greater in chisel till, rye and weeds with 0 or 60 kg N ha⁻¹ than in other treatments, but biomass N uptake was greater in vetch/rye with 60 kg N ha⁻¹ than in rye and weeds with 0 or 60 kg N ha⁻¹. Increased N supplied by hairy vetch or 120–130 kg N ha⁻¹ increased soil N availability, sorghum grain yield, cotton and sorghum biomass yields, and N uptake but decreased cotton lint yield and lint N uptake compared with rye, weeds or 0 kg N ha⁻¹. Cotton and sorghum yields and N uptake can be optimized and potentials for soil erosion and N leaching can be reduced by using conservation tillage, such as no-till or strip till, with vetch/rye biculture cover crop and 60–65 kg N ha⁻¹. The results can be applied in regions where cover crops can be grown in the winter to reduce soil erosion and N leaching and where tillage intensity and N fertilization rates can be minimized to reduce the costs of energy requirement for tillage and N fertilization while optimizing crop production.     

施氮量对不同品种小麦物质积累、转运及产量的影响

在大田条件下,以豫麦49-198、郑麦0943、百农418、许科316为试验材料,设置施纯氮120kg/hm ²（N1）、210kg/hm ²（N2）、300kg/hm ²（N3）3个施氮水平,比较分析不同施氮量对不同品种小麦干物质、氮素积累与转运以及产量的影响。结果表明：同一品种不同施氮量处理、同一施氮量不同品种间小麦干物质积累量、干物质转运规律和氮素积累量、氮素转运规律以及子粒产量存在显著差异。适当增施氮肥可以有效增加小麦植株群体干物质积累量,成熟期,豫麦49-198和郑麦0943在N2处理干物质积累量最高,百农418和许科316在N3处理干物质积累量最高。不同品种小麦营养器官花前贮藏干物质和氮素的转运量、转运率以及物质转运对子粒的贡献率均在N2处理达到最高。豫麦49-198和郑麦0943在N2处理获得最高产量,分别达8 036.67和6 873.33kg/hm ²,百农418和许科316在N3处理获得最高产量,分别为7 636.67和7 713.33kg/hm ²。因此,在小麦生产过程中,应根据不同品种合理施氮,实现高产。     

不同氮效率水稻品种叶片光合作用及氮利用特征的差异分析

以扬稻6（氮高效品种）、武玉粳3（氮低效品种）为材料,在含有2.86mmol/L NH₄ ⁺溶液培养条件下,分别在开花期和灌浆期分析了中位叶、旗叶的净光合速率、氮同化酶的活性、叶片氮含量与转移率以及对子粒的贡献。结果表明：（1）旗叶的净光合速率显著高于中位叶;扬稻6旗叶的净光合速率显著高于武玉粳3,而中位叶净光合速率在品种间差异不显著。（2）硝酸还原酶活性（NRA）、谷氨酰胺合成酶活性（GSA）是中位叶高于旗叶、灌浆期高于开花期、扬稻6高于武玉粳3。从开花到灌浆期,旗叶可溶性糖含量呈增加的趋势,而中位叶可溶性糖含量呈减少的趋势。旗叶可溶性糖含量高于中位叶、扬稻6高于武玉粳3。可溶性蛋白含量呈下降的趋势,扬稻6可溶性蛋白含量高于武玉粳3。扬稻6、武玉粳3中位叶可溶性蛋白的转出率分别是50.3%、37.6%;旗叶可溶性蛋白的转出率分别是69.5%、44.4%。（3）中位叶总氮积累量显著高于旗叶、开花期显著高于灌浆期。扬稻6中位叶、旗叶氮转移量分别为35.6%、34.7%;武玉粳3中位叶、旗叶氮转移量分别为26.9%、35.3%。（4）去除中位叶,子粒氮分别比对照减少了6.9%（扬稻6）、4.6%（武玉粳3）;子粒产量分别比对照减少了7.3%（扬稻6）、4.9%（武玉粳3）。去除旗叶,子粒氮分别比对照减少了10.5%（扬稻6）和9.2%（武玉粳3）;子粒产量分别比对照减少了13.8%（扬稻6）、11.1%（武玉粳3）,表明旗叶对子粒氮和产量的贡献更大。总之,氮高效品种旗叶光合作用和氮同化能力显著高于氮低效品种,另一方面,其中位叶储存的氮也能及时转移到子粒中,提高了氮效率,这反映了不同氮效率品种氮素利用差异的机制。