用二重PCR方法检测李痘病毒和李坏死环斑病毒

以我国两种重要的检疫性木本植物病毒李痘病毒和李坏死环斑病毒为对象,研究了二重PCR的检测方法,为我国口岸对这两种病毒的检疫提供了一条更为灵敏有效的新方法。     

Hosts and symptoms of Plum pox virus: ornamental and wild Prunus species

Several ornamental and wild Prunus species have been identified as natural and/or experimental hosts for Plum pox virus (PPV). The significance of natural vs. experimental hosts, graft or bud-transmitted infections vs. aphid-transmitted infections in ornamental or wild Prunus hosts, and their relevance in the field situation, are not clearly understood. However, since PPV is aphid-transmitted, any host in the field or nursery serves as a potential reservoir and source of inoculum and must be monitored and controlled in any PPV eradication or management programme.     

Identification of Plum pox virus Determinants Implicated in Specific Interactions with Different Prunus spp

ABSTRACT The characterization of pathogenic properties of two infectious clones of Plum pox virus (PPV) isolates, pGPPV (D group) and pGPPVPS (M group), was investigated in their woody hosts (seedlings of Prunus spp.). The two clones differed in their ability to infect plum and peach cultivars, from no infection to local and systemic infection. The phenotype determinants were located with a set of chimeric viruses from the two clones. In plum, determinants of systemic infection were located in a genomic fragment encoding the P3 and 6K1 proteins, which might influence genome amplification or virus movement. The capacity of pGPPVPS to induce stable local and systemic infections in peach was not located accurately and might be influenced by multiple determinants carried by different regions of the genome, excluding those encoding the protein 1, the majority of helper component, nuclear inclusions a and b, and coat protein. We conclude that PPV infections of plum and peach are governed by different determinants.     

李痘病毒及其风险分析

李痘病毒是危害核果类果树的重要病原物,具有传播迅速的特点,已被多个国家列为检疫性有害生物。本文对李痘病毒生物学特性等方面进行综述,通过分析该病毒的传播和定殖规律,并根据我国的具体生态情况,对其传入的可能性进行风险分析,结果认定其传入的可能性较大,需要制定严格的检疫措施,严防传入中国。