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1.
The rapid development of transgenic biotechnology has greatly promoted the commercialization of genetically modified (GM) crops including the insect-resistant crops worldwide. Apart from the enormous yield benefits brought by the GM crops, the cryptic fitness cost associated with transgenes has also been detected under experimental conditions although it is considered to be rare. To estimate the yield benefit and cost of insect-resistant GM rice, we studied field performances of three insect-resistant GM rice lines, involving their non-GM parental variety as comparison. Great benefits as estimated by the yield-related traits were observed in the GM rice lines when high insect pressure was recorded, but a cryptic yield loss was detected when the level of insect pressure was extremely low. Given the fact that cryptic yield loss presented in the GM rice lines under the low insect pressure, a strategic field deployment should be required when insect-resistant GM rice are commercialized to circumvent the unnecessary yield losses. This is probably true for other insect-resistant GM crops. Effective biotechnology and breeding measures are also needed to particularly minimize the potential underlying cost of an insect-resistance transgene before commercial production of the GM crops. 相似文献
2.
Understanding the potential for the evolution of herbicide tolerance in weeds following gene flow from transgenic crops is important for their management. The inter-specific hybrids formed between four Brassica juncea populations with different glyphosate-susceptibility and transgenic oilseed rape was studied, emphasizing on the possible population difference. Compared with both parents, the fitness-related components and photosynthetic capacity of hybrids decreased dramatically, but significant differences were observed among different hybrids. All F1 hybrids exhibited highly enhanced but similar herbicide tolerance level regardless of the wild B. juncea parent, indicating that genetically engineered herbicide tolerance may over-dominate phenotype ones by wild-crop hybridization. The analysis of parental loci transmission revealed a higher transfer ratio of male-specific loci detected in F1 hybrids, suggesting that oilseed rape genetic markers can be transferred at relatively high frequencies to the next generation. Therefore, higher transfer ratio of oilseed rape-specific loci, coupled with variation of populations in fitness-related parameters in F1 hybrids, could complicate environmental risk assessment of transgenic oilseed rape, especially in current agroecosystems with increasing application of glyphosate. 相似文献
3.
Agronomic traits contributing to nitrogen efficiency of winter oilseed rape cultivars 总被引:1,自引:0,他引:1
Gunda Schulte auf‘m ErleyWalter J. Horst 《Field Crops Research》2011,124(1):114-123
Reducing the high nitrogen (N) balance surpluses occurring in oilseed rape production through the cultivation of N-efficient cultivars is imperative for environmental reasons. In this study, seed yield and N efficiency parameters of oilseed rape cultivars grown under three N rates were investigated in field experiments performed during five years. Seed yields differed significantly among cultivars especially at limiting N supply, with significant shifts in cultivar ranking according to the N level. At high N supply, cultivar differences relied more on year effects. The importance of N efficiency parameters for yield varied owing to year and N rate effects. N uptake was an important parameter for yield at all N rates. While at low N, the duration of N uptake was most decisive, shoot N uptake capacity during vegetative growth was relatively more important under high N conditions. N utilization efficiency became more important with increasing N supply. At high N supply, cultivars with low seed N concentrations were superior in yield. Harvest index was also significantly correlated to yield across N rates; however, its importance depended much on environmental conditions. In conclusion, the specification of plant traits that might contribute to enhanced N efficiency in oilseed rape will depend very much on the N supply of the target environment and the target yield level. 相似文献
4.
5.
Herbivorous insects can demonstrate great selectivity among visual cues. The cabbage seedpod weevil, Ceutorhynchus obstrictus (Marsham) (Coleoptera: Curculionidae) (syn. Ceutorhynchus assimilis (Paykull)) is also influenced by visual cues. Here, we present results of a study examining the attractiveness of flowers and foliage of Sinapis alba (a suboptimal host for larval development), Brassica napus (an excellent host for larval development) and novel lines derived from S. alba x B. napus; several of these have been demonstrated to be resistant in field and laboratory tests. Attractiveness, reflectance properties and resistance demonstrated in field trials were related. Responses to host plants differed among plant genotypes and were related to the amounts of UV and yellow reflected from flowers. Moderate UV reflectance greatly increased the attractive effects of yellow. Fewer weevils responded to test plants with greater or lesser floral UV reflectance. 相似文献
6.
The insecticidal and residual effect of spinosad on wheat, maize and barley grain was evaluated in the laboratory against adults of Sitophilus oryzae (F.) (Coleoptera: Curculionidae), Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae), Tribolium confusum (DuVal) (Coleoptera: Tenebrionidae), Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae) as well as against larvae of T. confusum. Spinosad was applied as a solution to 2 kg lots of each commodity at three concentrations, 0.1, 0.5 and 1 ppm, and the treated grain quantities were kept at 25 °C and 65% RH. Samples were taken from each concentration-commodity combination at the day of storage and every 30 d for 6 consecutive months (6 bioassays). The test species were exposed for 14 d to the samples and mortality and reproduction were assessed over this exposure interval. With the exception of T. confusum, 1 ppm of spinosad was highly effective against the remainder of the tested species and provided protection for a period of storage at least 4 months. Although in general, spinosad performance was not very much affected by the grain type, efficacy on maize was less stable over the 6-month period of storage and declined sooner compared to the other commodities. Spinosad almost suppressed progeny production of R. dominica during the storage period, but did not suppress progeny of the other species, since progeny were recorded even 30 d post application especially with the lowest of the tested concentrations. The results of this study indicated that spinosad may provide suitable protection for 6 months against S. oryzae or R. dominica, but is not suitable for long-term protection against T. confusum or C. ferrugineus. 相似文献
7.
Transgenically expressed Bacillus thuringiensis insecticidal-protein Cry1Ab was quantified in target tissues of insect feeding of several YieldGard® corn hybrids. The Cry1Ab protein is intended to protect corn plants from two economically important stem borers, Chilo partellus and Sesamia inferens. A total of seven YieldGard hybrids, all with MON810 event, were field-tested in a total of fourteen locations during the dry season (October–March) of 2005/2006 and wet season (May–October) of 2006. S. inferens and C. partellus oviposit on leaves of young corn plants, 15–60 days after emergence (DAE). The neonates initially feed by scraping the leaf lamina before migrating to bore into the stem. Thus high concentrations of Cry1Ab in whorl leaf and stem tissues would ensure effective control of the borers. The mean tissue Cry1Ab concentrations during the oviposition window of the borers (15–60 DAE), ranged from 50.05 to 21.01 ppm in whorl leaf, and between 9.26 and 3.47 ppm in stem tissue during the same period in the dry season of 2005/06. Similarly, Cry1Ab concentrations in whorl leaf and stem between 15 and 60 DAE during the wet season of 2006 ranged between 19.30 to 11.08 and 14.28 to 4.69 ppm, respectively. The baseline-sensitivity data of these insects to Cry1Ab in laboratory assays was determined. The concentrations of Cry1Ab in the target tissues as studied in seven YieldGard hybrids tested suggest effective management of the two borers. This paper also provides a summary of the expression of the Cry1Ab gene in various genetic backgrounds. 相似文献
8.
A highly efficient regeneration protocol for oilseed crop Crambe abyssinica has been developed using hypocotyls as explants in this study. Crambe is a potential engineering oilseed crop for industrial purposes as it contains 55-60% erucic acid in its oil and, more importantly, it does not outcross with any food oil seed crops. However, the low regeneration frequency with the currently available protocols is still a limiting factor for genetic modification of Crambe. In this study, we investigated the effects of N-source, C-source, AgNO3, cultural conditions as well as the concentration and combination of plant growth regulators (PGR) on the regeneration frequency of C. abyssinica. The results showed that all these factors, especially the N-source and PGR concentrations and combinations, played an important role in shoot regeneration. Among all the factors tested, the combination of using hypocotyls from C. abyssinica cv. galactica, the Lepiovre basal medium supplemented with 16 g l−1 glucose, 0.5 g l−1 AgNO3, 2.2 mg l−1 thidiazuron (TDZ), 0.5 mg l−1 α-naphthaleneacetic acid (NAA), 2.5 g l−1 Gelrite, seeds germinated in dark for 3 days and explants cultured in light, gave the best regeneration frequency (over 95%). The results also suggest that reducing the content of NH4+ or keeping a suitable NO3−/NH4+ ratio in the regeneration medium would be crucial to Crambe shoot regeneration. 相似文献
9.
The reactions to shot-hole disease (Stigmina carpophila) of nine plum cultivars and seed-propagated myrobalan trees were evaluated during three years in three commercial orchards located at Nova Siri, Matera, Italy. In July 2006, May 2007, June 2007 and June 2008, shot-hole severity and twig defoliation were assessed. Pathogen pressure was higher in 2007 and 2008 than in 2006. In the three years of study, cultivar Golden Plumza was the most susceptible to the shot-hole measured by leaf severity, however it showed variable defoliation percentages in different years. The seed-propagated myrobalan trees were the most resistant, although they showed a slight degree of defoliation in 2007 and 2008. Intermediate reactions to the disease occurred in cultivars Angeleno, Autumn Giant, Fortune, Green Sun, October Sun, Santa Rosa, Sorriso di Primavera and T.C. Sun. A significant correlation between shot-hole leaf severity and twig defoliation was detected. To our knowledge, this is the first report on screening for resistance of plum cultivars to shot-hole disease. 相似文献
10.
Ann Blechl Jeanie LinSon Nguyen Ronald ChanOlin D. Anderson Frances M. Dupont 《Journal of Cereal Science》2007
To test the effects of independently increasing the in vivo levels of high-molecular-weight glutenin subunits (HMW-GS) Dx5 and Dy10 on wheat flour properties, we increased the copy numbers of their corresponding genes by genetic transformation. Thirteen transformants with increases in one or both subunits were chosen for biochemical and functional characterization by solvent fractionation, RP-HPLC, SDS-sedimentation, and micro-mixing. Increases in Dx5 and Dy10 contents ranged from 1.4- to 3.5-fold and 1.2- to 5.4-fold, respectively, and generally resulted in increased polymeric protein, increased mixing times and tolerances, and lower peak resistances. Increases in Dx5 content had larger effects on most parameters than comparable increases in Dy10. Flours with more than 2.6-times the native levels of Dx5 could not be mixed under standard 2-g mixograph conditions, while flours with 5.4 times the native levels of Dy10 could be mixed if sufficient time was allowed. Increases in Dx5 and Dy10 had additive effects on mixing behavior. These experiments demonstrate that dough mixing strength and tolerance can be increased by raising the levels of native HMW-GS Dx5 or Dy10, but that the effects of the two subunits are quantitatively and qualitatively different. 相似文献
11.
White rust (Albugo candida) is a highly destructive disease of oilseed Brassicas such as Brassica juncea and B. rapa, and has caused serious yield losses in Australia, China and India on both species. The first commercial B. juncea varieties are now being deployed in Australia, but their response to Australian strains of A. candida is yet to be defined under Australian field conditions. To identify useful sources of host resistance for Australia, China and India, in B. juncea, three field trials were undertaken in Western Australia. Forty-four B. juncea genotypes, viz. 22 from India, 12 from Australia and 10 from China, were tested. Varying levels of host resistance to Australian strains of A. candida (race 2) were identified among the genotypes from the three countries. Genotypes CBJ-001, CBJ-003 and CBJ-004 from China consistently showed high levels of resistance to A. candida on leaves across the three trials. Overall, the genotypes from China showed the best resistance, followed by the genotypes from Australia, with those from India being the most susceptible. The most susceptible genotypes were RL1359, RH30 and Seetha from India. It is noteworthy that both the incidence and severity of disease reflected varying levels of host resistance in the germplasm from the three countries, irrespective of whether screening was undertaken in the field using natural or artificial inoculation. Differentiation of resistance among these genotypes was similar to that we reported previously for artificially-inoculated seedlings or adult plants under glasshouse conditions, indicating that a choice of options is available to plant breeders to reliably differentiate host resistance among genotypes to white rust in B. juncea. 相似文献
12.
Gema P. Farinós Stefanos S. AndreadisMarta de la Poza George K. MironidisFélix Ortego Mathilda Savopoulou-SoultaniPedro Castañera 《Crop Protection》2011,30(7):902-906
A three year (2003-2005) field study compared the susceptibility to the Cry1Ab toxin, expressed in Bt maize, of Mediterranean corn borer (MCB) Sesamia nonagrioides populations collected from areas with different adoption rates of Bt maize in Spain with Bt-free areas in Greece. Spain is the only European country where the cultivar Compa CB derived from the event Bt176 was commercially grown, from 1998 to 2005. The large decrease of the titer of the toxin in this cultivar at later growth stages represented the worst-case scenario for resistance development of MCB, since larvae of the second and third generations were exposed to sublethal concentrations of Cry1Ab toxin. Our data revealed that the variation in susceptibility to Cry1Ab for the MCB Spanish field populations analyzed in the three years was very low, with LC50 values fluctuating between 12 and 30 ng Cry1Ab/cm2, regardless of the region of origin, the type of maize (Bt or non-Bt) and the year. Furthermore, no significant differences were found when comparisons were made with a laboratory population (LC50 values: 18-26 ng Cry1Ab/cm2) or with field populations from Greece (Bt-free areas), which displayed LC50 values ranging between 22 and 27 ng Cry1Ab/cm2. Standardizing bioassay protocols proved to be essential for obtaining comparable results. These findings suggest that resistant MCB populations did not evolve in those Spanish maize areas where Compa CB was largely cultivated for eight years, contradicting the expected rapid development of resistance under these unfavourable conditions. Additionally, our results can be used as baseline indices in post-market resistance monitoring programs if Bt maize is introduced in Greece. Further studies should continue, since the insights gained from a resistance monitoring program may help to enhance the durability of Bt maize. 相似文献
13.
Dalila Haouas Flamini Guido Ben Halima-Kamel Monia Ben Hamouda Mohamed Habib 《Industrial Crops and Products》2011,34(1):1128-1134
Compounds responsible for insecticidal properties of Chrysanthemum macrotum (D.R.) Ball. leaves against Spodoptera littoralis Boiduval caterpillars have been investigated. The screening of the insecticidal activity was performed by incorporating methanol, buthanol or ethyl acetate extracts, or some chromatographic fractions to the caterpillars’ artificial diet. It was noted that extracts and fractions ameliorated or disturbed nutritional indexes, being not always toxic for caterpillars. Among the tested fractions, one pure compound with a high insecticidal activity (percentage of mortality 66.7%) was purified. The nuclear magnetic resonance study allowed its identification as a polyacetylene derivative, in particular a spiroketal enol ether one. 相似文献
14.
Integrated pest management in faba bean 总被引:1,自引:0,他引:1
F.L. Stoddard A.H. Nicholas D. Rubiales J. Thomas A.M. Villegas-Fernández 《Field Crops Research》2010
Successful production of faba bean crops in the presence of a wide range of disease-causing fungi, parasitic weeds, nematodes, insects, mites and other pests depends on the integration of genetic resistance, hygienic management, monitoring of the target organisms and timely application of appropriate chemical and biological treatments. This paper reviews the progress in this area. 相似文献
15.
The present investigation was conducted at Vittal, Karnataka, India during 2004-2007 to study the feasibility of intercropping of medicinal and aromatic plants (MAPs) in arecanut plantation. The results revealed that MAPs can be successfully grown as intercrops in arecanut plantation with increased productivity and net income per unit area. Kernel equivalent yield of MAPs varied between 272 kg ha−1 in case of Piper longum to 1218 kg ha−1 in Cymbopogon flexuosus. Pooled data indicated that Asparagus racemosus produced fresh root yield of 10,666 kg ha−1 of arecanut plantation and contributed to maximum kernel equivalent yield of 1524 kg ha−1 among all medicinal and aromatic plants. Intercropping of MAPs in arecanut was found economical. The net return per rupee investment was highest in C. flexuosus (4.25) followed by Bacopa monnieri (3.64), Ocimum basilicum (3.46) and Artemisia pallens (3.12). The total system productivity of arecanut + MAPs intercropping system varied from 2990 to 4144 kg ha−1. Arecanut + O. basilicum intercropping system registered significantly higher production efficiency 8.2 kg ha−1 day−1 than other systems. Intercropping of MAPs had more positive effect on soil pH in arecanut based cropping system. The soil pH was 5.6 in 2004 and it was 0.3-0.9 units higher in 2007. Soil organic carbon (SOC) content varied significantly due to intercropping of MAPs at the end of experiment. The SOC content increased in Aloe vera, A. pallens, P. longum and B. monnieri, while it depleted in grasses and rhizomatic MAPs. Based on demand and marketing opportunities for MAPs, farmers are advised to grow aromatic plants in large areas on a community basis to meet huge industrial demand and variety of medicinal crops in small areas to meet the requirement of traditional systems of medicine. 相似文献
16.
In 2009, a survey was conducted of cereal fields in five prefectures of Greece to establish the frequency and distribution of herbicide-resistant sterile wild oat (Avena sterilis L.). In total, 104 sterile wild oat accessions were collected and screened in a field experiment (conducted in 2009 and repeated in 2010) with several herbicides commonly used to control this weed. Most of the sterile wild oat accessions (89%) were classed as resistant (or developing resistant) to the ACCase-inhibiting herbicide diclofop, while resistance to other ACCase-inhibiting herbicides was markedly lower. The results of the pot experiments showed that some of the sterile wild oat accessions were found to have a very high level of diclofop resistance (resistance index up to 28.6), while cross-resistance with other herbicides was common. The levels of resistance and cross-resistance patterns varied among biotypes with different amount and time of selection pressure, indicating either more than one mechanism of resistance or different resistance mutations in these sterile wild oat biotypes. LA14, which had the highest diclofop resistance level (28.6 resistance index), showed resistance to all APP herbicides applied and non-ACCase inhibitors. Alternative ACCase-inhibiting herbicides, namely tralkoxydim and pinoxaden remain effective on 86 and 92% of the tested sterile wild oat populations, respectively. For the ALS-inhibiting herbicide mesosulfuron + iodosulfuron, nearly all the sterile wild oat accessions were susceptible (97%), with only 3 of them classed as developing resistance. Therefore, there is an opportunity to effectively control sterile wild oat by selecting from a wide range of herbicides and other cultural practices. Early post-emergence herbicide application is strongly suggested, since it could decrease the number of resistant seeds in the field and slow down the dispersal of this major problem. 相似文献
17.
The nematicidal effect of Pseudomonas fluorescens, Paecilomyces lilacinus, Pichia guilliermondii and Calothrix parietina singly or in combination was tested against root-knot nematode, Meloidogyne incognita. Treatments with P. fluorescens and P. lilacinus caused mortality of M. incognita as 45% and 30% of juveniles after 48 h of exposures, respectively compared to water control in vitro. Under greenhouse conditions, all treatments reduced the disease severity and enhanced plant growth compared to untreated control. Application of P. fluorescens, P. lilacinus and P. guilliermondii Moh 10 was more effective compared to C. parietina. There was a negative interaction between C. parietina and either P. lilacinus or P. guilliermondii. Fresh and dry weight of shoots and roots of plants were significantly reduced as a result of infection with M. incognita, however application of biocontrol agents singly or in mix recovered this reduction. Moreover, they enhanced the growth parameters compared with the control. Our results proved that application of different biocontrol agents (P. fluorescens, P. lilacinus and P. guilliermondii) not only has a lethal effect on nematode, but also enhances the plant growth, supplying many nutritional elements and induction the systemic resistance in plants. Presence of C. parietina as a soil inhabitant cyanobacterium could antagonize biocontrol agents leading to the reduction of their practical efficiency in soil. 相似文献
18.
Mukti N. GhimireFangneng Huang Rogers LeonardGraham P. Head Yunlong Yang 《Crop Protection》2011,30(1):74-81
Multiple independent trials were conducted to evaluate the performance of Cry1Ab-susceptible (Cry1Ab-SS), -heterozygous (Cry1Ab-RS), and -resistant (Cry1Ab-RR) genotypes of the sugarcane borer, Diatraea saccharalis (F.), on eight commercial hybrids and six experimental corn lines. The commercial varieties included two non-Bt and six Bt corn hybrids that expressed a single Bt protein (either Cry1Ab or Cry1F) targeting above-ground lepidopteran pests. The six experimental lines consisted of two non-Bt and four Bt corn lines, two expressing just the Cry1Ab protein and two containing the pyramided-genes Cry1A.105 and Cry2Ab2 (event MON 89034). Larval mortality on non-Bt corn leaf tissue ranged from 6 to 45% after 12 d across insect genotypes. The 12 d mortality of Cry1Ab-SS on leaf tissue of commercial Cry1Ab or Cry1F corn was 96-100%, whereas it was 80-96% for Cry1Ab-RS and 68-78% for Cry1Ab-RR. On intact plants, 39-64% of larvae survived on non-Bt corn plants after 21-25 d. Larval survivorship on intact plants of commercial Cry1Ab or Cry1F corn was 0-8.1% for Cry1Ab-SS, 1.3-34% for Cry1Ab-RS, and 19-51% for Cry1Ab-RR. Larvae of Cry1Ab-RR and -RS also caused significant plant injury to most of the commercial Bt corn hybrids, especially to the Cry1Ab corn. Cry1Ab resistance in D. saccharalis was incompletely dominant on commercial Bt corn hybrids. However, both experimental lines with pyramided genes of Cry1A.105 and Cry2Ab2 provided complete control of all three insect genotypes in both leaf tissue and intact plant tests. Results of this study suggest that MON 89034 should offer a means for Bt resistance management in D. saccharalis. 相似文献
19.
Significant areas of rain-fed rice in the Sahel, savannah and derived savannah zones of sub-Saharan Africa (SSA), Madagascar and other Indian Ocean Islands are infested by parasitic weeds. The affected area accommodates some of the poorest farmers of the world. Without appropriate management parasitic weeds in rice are expected to increase in importance in SSA due to their general invasive nature and their abilities to adapt to changing conditions such as imposed by predicted climate changes. The most important parasitic weeds in rice are: Striga hermonthica, Striga asiatica, Striga aspera and Rhamphicarpa fistulosa. The first two are primarily found in free-draining uplands while S. aspera is also found on hydromorphic soils and R. fistulosa is restricted to unimproved lowlands including inland valleys. As parasitic weeds are typical production constraints in subsistence rice production, targeting them would directly contribute to poverty alleviation and food security. This paper provides an overview of the problems caused by parasitic weeds in rice and discusses management options and opportunities for research for development. 相似文献
20.
The commercial mycopesticide, Vertalec® based on Lecanicillium longisporum, was evaluated for simultaneous control of aphids and powdery mildew on cucumbers in a greenhouse setting where temperature and RH were allowed to fluctuate within normal operating ranges. Five to six week old cucumber plants were inoculated with either Sphaerotheca fuliginea (Sf) spores, cotton aphids (Aphis gossypii) or both. Vertalec, Vertalec containing irradiation-inactivated blastospores (II Vertalec) or sterilized water (control) were applied to the plants 1, 4, and 7 days later. Vertalec treatment provided complete control of aphids 16 days after aphid inoculation, whereas effects of the II Vetalec were not significantly different from the water-treated control. The number of powdery mildew spots on cucumber leaves and the number of S. fuliginea spores in each spot were significantly lower in Vertalec-treated plants than II Vertalec-treated plants or the controls, whereas numbers in the II Vertalec treatment were lower that the untreated control. These results demonstrate that Vertalec has potential for simultaneous management of both cotton aphid and powdery mildew in greenhouse cucumber production. 相似文献