Effects of a one-year rainfall manipulation on soil nematode abundances and community composition

Soil nematodes play a crucial role in the terrestrial nitrogen cycle by accelerating the release of ammonium from microorganisms (bacteria and fungi). As aquatic organisms, nematodes are likely to be affected by predicted changes in precipitation patterns and soil moisture during the 21st century. The objective of this study was to measure the response of soil nematodes to a one-year rainfall manipulation in the sandy, forest soils of the New Jersey Pinelands (USA). We excluded all rain from four replicate field plots and applied double the amount of natural rainfall to four additional plots. We then assessed the impact of these precipitation treatments on nematode abundance and community composition. We found that total nematode abundance increased with more precipitation, and were highly sensitive to annual precipitation amount. This is in contrast to microbial biomass which was previously found to be insensitive to precipitation change. We suggest that any increased microbial growth in high rainfall plots was consumed by microbivorous nematodes. We further suggest that nematodes in the freely draining, sandy soils we studied may be unsuccessful at surviving drought because few water-filled pore spaces remain, as compared to more aggregated soils. All nematode families were sensitive to drought, but the effect was greatest on the Plectidae, while no significant effects were found for the Cephalobidae and Qudsianematidae. While not directly measured, these results provide insight into the relative anhydrobiotic abilities of these families. We found that bacterial-feeding nematodes were most sensitive to drought, suggesting that grazer-induced alterations to the nitrogen cycle are possible if precipitation patterns change in the future.     

Seasonal changes in the microbial biomass of Brazilian soils with different organic matter contents

Abstract

Carbon and nitrogen levels of microbial biomass were studied in four plots located in Rio de Janeiro State, Brazil. Two samplings were carried out, the first one, on November 1992 when rainfall was high which led the soil to high levels of moisture, and the second one on March 1993 when there was a decrease in rainfall coupled with high temperatures. Microbial carbon (MBC) and microbial nitrogen (MBN) assessments were done by the fumigation‐extraction method. The results showed significant differences for MBC and MBN between the sampling times and between different plots. Moreover, MBN showed differences as a function of sampling depth. In the 1993 sampling, developed under moisture conditions of soil which promoted the mineralization of organic matter, lesser values of MBC and MBN were found, whereas there was no difference in the organic carbon content. This fact shows a major sensitivity of biomass measurements to reflect changes which occur in the soil organic matter content. Significant correlations were obtained between MBC and organic carbon (r = 0.35, P < 0.01, n = 68), MBN and total N (r = 0.62; P < 0.07, n = 47), and MBC with NBM (r 0.74, P < 0.01, n = 54).     

Influence of drying of the samples on the transformation of nitrogen and carbon compounds in mountain-meadow alpine soils

The drying of samples of mountain-meadow soils characterized by their permanently high moisture under natural conditions fundamentally changes the concentrations of the labile nitrogen and carbon compounds, as well as the patterns of their microbial transformation. When the soil samples are dried, a four- to fivefold increase in the content of the extractable organic nitrogen compounds, carbon compounds, and inorganic nitrogen compounds is observed, while the content of nitrogen and carbon of the microbial biomass decreases by two-three times. The rewetting of the dried soil launches the process of the replenishment of the nitrogen and carbon reserves in the microbial biomass. However, even after two weeks of incubation, their values were 1.5–2 times lower than the initial values typical of the natural soil. The restoration of the microbial community in the samples of the previously dried soils occurs in the absence of a deficiency of labile organic compounds and is accompanied by their active mineralization and the low uptake of ammonium nitrogen by the microorganisms.     

Legacies of organic matter removal: decreased microbial biomass nitrogen and net N mineralization in New Zealand Pinus radiata plantations

The effect of variations in organic matter removal during harvesting on microbial biomass nitrogen was determined in four Pinus radiata plantations between 9 and 17 years after harvesting. Variation in microbial biomass nitrogen with season and the response of net nitrogen mineralization to organic matter removal after 9 and 17 years were also determined at two of the sites. The microbial biomass nitrogen in the fermentation-humus (FH) layer was correlated with litter fall characteristics and did not vary with organic matter removal, but the total mass of microbial biomass nitrogen in the FH layer was significantly reduced by increased organic matter removal. The microbial biomass nitrogen in the mineral soil was decreased by increased organic matter removal and was strongly correlated with moisture content and total nitrogen concentration. The FH layer microbial biomass nitrogen did not vary with season, but mineral soil microbial biomass nitrogen varied with season at one site, probably due to increased moisture availability. Net nitrogen mineralization in the mineral soil, determined by an anaerobic incubation, was decreased by increased organic matter removal and was strongly correlated with microbial biomass nitrogen. The persistence of the significant differences in microbial biomass nitrogen and net nitrogen mineralization indicated that variations in organic matter removal during harvesting have long-term effects on soil microbial properties and activity during the life of the subsequent rotation. This has implications for the selection of harvesting techniques to promote the maintenance of site productivity and to minimise disruption to the soil biota.     

ABSTRACT of the Articles Printed in Journal of the Science of Soil and Manure,Japan

Changes in soil moisture determine the reproductive, respiratory, and metabolic activities of soil microorganisms and hence the rate of microbial nitrogen (N) mineralization. Soil moisture also affects the feeding activity and movement of soil invertebrates. Bacterial and fungal grazing by soil invertebrates such as Collembola and nematodes is known to increase N mineralization by increasing the reproductive, respiratory, and metabolic activities of microorganisms. Therefore, to assess the effect of soil moisture on N mineralization, faunal responses need to be considered. We used microcosms to investigate the effect of soil moisture on N mineralization mediated by a species of Collembola, Folsomia candida Willem. We used four moisture levels corresponding to matric potentials of ?42.5, ?11.8, ?0.8, and ?0.5 kPa and investigated the effects of these on Collembola with respect to feeding activity, growth, and contribution to N mineralization. The microbial biomass and ratio of bacterial to fungal biomass tended to increase with increasing soil moisture. Collembola feeding activity and growth increased with increasing soil moisture conditions. Collembola significantly enhanced N mineralization in soil at water potentials of ?11.8 and ?0.5?kPa. The greatest relative increase in N mineralization attributed to Collembola occurred in the ?11.8?kPa treatment. The change in contribution of the Collembola to N mineralization with soil moisture was most likely induced by changes in Collembola feeding activity and microbial community structure. The growth in body length of the Collembola was significantly greater at higher moisture conditions than at the lowest moisture condition, indicating that increases in both metabolic activity and biomass of the Collembola population contributed to the enhanced N mineralization.     

Microbial biomass and N mineralization in relation to N supply of sugar beet under reduced tillage

Reduced tillage may affect N supply of plants by influencing soil microbial biomass and thereby N release. The aim of this study was to evaluate changes in microbial biomass due to tillage in relation to N mineralization and to assess the contribution to the N supply of sugar beet. For this purpose, in a field trial near Göttingen in 1995 microbial biomass and net N mineralization were determined in an in situ incubation of ploughed and reduced tilled soil in plots which were not given application of mineral N fertilizer. In reduced tilled soil the increase in mineral N concentration in the upper 10 cm of soil was mainly attributed to an increase in microbial biomass. The organic matter was more easily decomposable, indicated by the increase in C_mic/C_org and N_mic/N_t ratios; this was further supported by the enhanced turnover of microbial biomass in reduced tillage plots. A regression function was used to relate seasonal fluctuations of microbial biomass, soil moisture and soil temperature to N mineralization rate. There was a good agreement between measured and calculated N mineralization rate. Reduced tillage affected N mineralization by affecting the quantity and quality of microbial biomass. In 0–30 cm soil depth 169 kg N/ha were mineralized, 30 kg more N than in ploughed soil. However, despite improved N availability, the N uptake of sugar beet was decreased in reduced tilled soil. Because the N concentration in plants did not differ, it was concluded that sugar beet growth in reduced tilled soil was impaired due to other factors than N supply.     

Wildfire effects on the properties and microbial community structure of organic horizon soils in the New Jersey Pinelands

The frequency and intensity of wildfires are expected to increase in the coming years due to the changing climate, particularly in areas of high net primary production. Wildfires represent severe perturbations to terrestrial ecosystems and may have lasting effects. The objective of this study was to characterize the impacts of wildfire on an ecologically and economically important ecosystem by linking soil properties to shifts in microbial community structure in organic horizon soils. The study was conducted after a severe wildfire burned over 7000 ha of the New Jersey Pinelands, a low nutrient system with a historical incidence of fires. Soil properties in burned and non-burned soils were measured periodically up to two years after the fire occurred, in conjunction with molecular analysis of the soil bacterial, fungal and archaeal communities to determine the extent and duration of the ecosystem responses. The results of our study indicate that the wildfire resulted in significant changes in the soil physical and chemical characteristics in the organic horizon, including declines in soil organic matter, moisture content and total Kjeldahl nitrogen. These changes persisted for up to 25 months post-fire and were linked to shifts in the composition of soil bacterial, fungal and archaeal communities in the organic horizon. Of particular interest is the fact that the bacterial, fungal and archaeal communities in the severely burned soils all changed most dramatically during the first year after fire, changed more slowly during the second year after the fire, and were still distinct from communities in the non-burned soils 25 months post-fire. This slow recovery in soil physical, chemical and biological properties could have long term consequences for the soil ecosystem. These results highlight the importance of relating the response of the soil microbial communities to changing soil properties after a naturally occurring wildfire.     

半干旱区不同围封年限对草地土壤和微生物碳氮含量的影响

[目的]探究半干旱区草地根际土壤碳氮及土壤微生物量碳氮对不同封禁年限响应特征,为半干旱草地生态系统物质循环研究以及生态系统养分限制判定等提供依据,并为确定合理围封年限提供科学参考.[方法]以宁夏回族自治区固原市云雾山国家级自然保护区半干旱草原为研究对象,应用生态化学计量学方法对比分析放牧地与围封10,25,35 a样地...     

Soil‐moisture change caused by experimental extreme summer drought is similar to natural inter‐annual variation in a loamy sand in Central Europe

Intensification of weather extremes is currently emerging as one of the most important facets of climate change. Research frontiers are in analyzing (1) the consequences for the hydrological cycle and (2) the effects of multifactor scenarios on ecosystems. However, in all theoretical and experimental scenarios, challenges arise as to how precipitation regimes translate into variation in soil moisture. Here, we explore soil‐moisture response to experimental changes in the precipitation regime in Central Europe over a period of 5 y, particularly focusing on the effects of recurrent extreme weather events. Intraannual difference in weekly precipitation sums imposed by extreme‐drought or heavy‐rainfall manipulations clearly exceeded interannual variation in the ambient precipitation pattern during the growing season between 2005 and 2009. However, soil‐moisture variability in the experimental plots did not clearly reflect any altered patterns in response to the manipulated precipitation regime. Natural variation in soil moisture between years was similar to within‐season differences between manipulations. Strong differences in soil‐moisture dynamics during the growing season can, however, be generated by changing the temporal distribution of rainfall events while keeping the magnitude of the precipitation sum constant. Our findings confirm a common methodological dilemma in precipitation‐change experiments searching for a logical way to determine how precipitation change affects communities and ecosystems on relatively short time scales: Alteration of weather regimes according to extreme‐value statistics and future scenarios vs. systematic alteration of soil moisture. For Central Europe, our data suggest that other factors rather than the magnitude of rainfall exclusion or addition would appear to be decisive for ecosystem response to more extreme precipitation regimes. Response of soil moisture to frequency, return interval, and timing of events is a promising approach for further exploration. In addition, buffer capacity of the ecosystem under study has to be taken into account.     

模拟增温和降水调控对土壤微生物碳氮、群落基质利用模式及群落组成的短期影响

Soil microorganisms are major drivers of soil carbon(C) cycling;however,the response of these microorganisms to climate change remains unclear.In the present study,we investigated how 18 months of multifactor climate treatments(warmed air temperature by 3℃ and decreased or increased precipitation manipulation by 30%) affected soil microbial biomass C and nitrogen(N),community substrate utilization patterns,and community composition.Decreased and increased precipitation significantly reduced microbial biomass C by 13.5% and 24.9% and microbial biomass N by 22.9% and 17.6% in unwarmed plots,respectively(P0.01).Warming enhanced community substrate utilization by 89.8%,20.4%,and 141.4% in the natural,decreased,and increased precipitation plots,respectively.Particularly,warming significantly enhanced the utilization of amine and carboxylic acid substrates among all precipitation manipulation plots.Compared with the natural air temperature with natural precipitation treatment,other treatments affected fungal community richness by -0.9% to 33.6% and reduced the relative abundance of the dominant bacterial and fungal groups by 0.5% to 6.8% and 4.3% to 10.7%,respectively.The warming and/or precipitation manipulation treatments significantly altered Zygomycota abundance(P0.05).Our results indicate that climate change drivers and their interactions may cause changes in soil microbial biomass C and N,community substrate utilization patterns,and community composition,particularly for the fungal community,and shifts in the microorganism community may further shape the ecosystems function.     

不同土地利用方式下降雨对土壤微生物量和呼吸的影响

全球气候变化会导致陆地生态系统干旱频繁,强降雨增多,深入研究降雨对土壤微生物量和呼吸的影响,有利于理解陆地生态系统中土壤碳、氮的循环.研究以北京市延庆县上辛庄水土保持科技示范园内的标准径流小区为对象,探讨不同土地利用方式下降雨对土壤微生物量和呼吸的影响及差异.结果表明,不同土地利用方式下土壤干旱时,降雨使土壤微生物量和土壤呼吸产生激增效应.2010年8月3日降雨后经果林、裸地、农用地的土壤微生物量碳与干旱期的相比分别增加了0.40,1.51,1.95倍;土壤微生物量氯与干旱期的相比分别增加了1.77,1.83,3.7倍;土壤呼吸与干旱期的相比分别提离了12.4%,12.5%,20.5%.激增幅度依次为农用地>裸地>经果林.农用地的土壤微生物量和土壤呼吸值均低于经果林、裸地的,但是降雨使其产生的激增幅度明显大于经果林和裸地的.     

降雨对不同土地利用类型土壤水氮变化特征的影响

以2018年6—10月降雨条件下园地、林地、荒草地、坡耕地和裸地的标准径流小区为研究对象,裸地为对照,通过研究降雨对园地、林地、荒草地、坡耕地和裸地的土壤含水率、总氮、硝态氮和铵态氮含量与土层深度和时间的变化特征,经野外试验数据统计分析,提出降雨对园地、林地、荒草地、坡耕地和裸地土壤含水率、总氮、硝态氮和铵态氮含量与土层深度和时间变化特征的影响。结果表明:降雨增加园地、林地、荒草地、坡耕地和裸地土壤含水率,加速土壤总氮、硝态氮和铵态氮水解转化硝化和反硝化速度,影响土壤含水率、总氮、硝态氮和铵态氮含量,降雨与土壤含水率、总氮、硝态氮、铵态氮呈显著相关性(P0.05)。降雨条件下园地、林地、荒草地、坡耕地和裸地的土壤含水率随土层深度增大而增大,土层深度100 cm处土壤含水率最大,分别为30.34%,27.67%,24.98%,24.03%和21.95%,总氮随土层深度增大呈先增大后减小,在土层深度为60 cm土壤总氮含量最大,分别为1.02,0.99,0.90,0.86,0.75 g/kg,硝态氮和铵态氮含量随土层深度增大而减小,在土层深度为100 cm硝态氮和铵态氮含量均最小,其中硝态氮含量分别为9.01,7.89,7.25,6.10,5.22 mg/kg,铵态氮含量分别为9.41,9.14,6.40,5.38,4.37 mg/kg。土壤含水率随时间的延长先减小后增大又减小,呈正余弦变化趋势,8月土壤含水率最大,分别为22.97%,22.01%,19.87%,19.03%和17.98%,总氮随时间的延长先增大后减小,8月总氮最大,分别为1.09,1.01,0.94,0.84,0.76 g/kg,硝态氮和铵态氮含量随时间的延长而逐渐减少,6月硝态氮和铵态氮含量均最大,其中硝态氮含量分别为13.40,12.37,11.20,10.39,8.67 mg/kg,铵态氮含量分别为18.89,17.02,14.54,12.02,8.36 mg/kg。不同土地利用类型土壤含水率、总氮、硝态氮和铵态氮平均值与土层深度和时间关系由大到小依次为园地、林地、荒草地、坡耕地和裸地,研究结果为农田土壤水肥流失控制和养分利用提供理论技术支持。     

硝化抑制剂和秸秆对潮棕壤碳氮转化和微生物群落特征的短期影响

氮是植物和微生物生长繁殖的必需营养元素,而氮矿化表征了土壤供氮能力。通过盆栽实验,采用同位素稀释法和磷脂脂肪酸(PLFA)法,研究了添加硝化抑制剂和秸秆条件下,潮棕壤碳氮矿化和微生物群落组成变化特征。结果表明,与施氮量N 0.1 g·kg~(-1)的单施氮肥处理(NF)相比,氮肥配施1%硝化抑制剂(NFI)的土壤铵态氮提高32%,而硝态氮降低53%。氮肥与施用量为5 g·kg~(-1)的秸秆配施(NS),土壤氮素总矿化速率增加36%,微生物生物量碳提高51%,β-葡萄糖苷酶活性提高36%,同时显著增加了土壤总PLFA以及细菌、真菌、真菌/细菌和革兰式阴性菌(P0.05),土壤呼吸熵降低50%。与氮肥配施秸秆处理(NS)相比,氮肥、秸秆和硝化抑制剂配施处理(NSI),土壤铵态氮提高33%,硝态氮下降47%。综上所述,氮肥和秸秆配施可以提高土壤微生物生物量,改变土壤微生物群落组成,配施1%(N)硝化抑制剂后降低土壤硝化速率,增加土壤供氮能力。     

添加生物质炭对红壤水稻土有机碳矿化和微生物生物量的影响

通过室内培育试验,研究了添加生物质炭对江西红壤水稻土有机碳矿化和微生物生物量碳、氮含量的影响。结果表明:红壤有机碳矿化速率在培育第2天达最大值后迅速降低,培养7天后下降缓慢并趋于平稳;添加生物质炭降低了土壤有机碳的矿化速率和累积矿化量,培养结束时,不加生物质炭的对照处理中有机碳的累积矿化量分别比添加0.5%和1.0%生物质炭的处理高10.0%和10.8%。此外,生物质炭的加入显著提高了土壤微生物生物量,添加0.5%生物质炭处理的土壤微生物生物量碳、氮含量分别比对照高111.5%～250.6%和11.6%～97.6%,添加1.0%生物质炭处理的土壤微生物生物量碳、氮含量分别比对照高58.9%～243.6%和55.9%～110.4%。相同处理中,干旱的水分条件下(40%田间持水量)微生物生物量要高于湿润的水分条件(70%田间持水量)。同时,添加0.5%和1.0%的生物质炭使土壤代谢熵分别降低2.4%和26.8%,微生物商减少了43.7%和31.7%。     

Organic matter dynamics in a temperate forest soil following enhanced drying

Climate models predict an increase in global surface temperature and a change in precipitation intensity during this century. For Europe, extended drought periods followed by heavy rainfall are expected. The consequences for soil organic matter (SOM) dynamics are poorly understood. In this study, we investigated the effect of changing soil moisture regime on SOM quality under field conditions. For this purpose, a throughfall exclusion (TE) experiment was conducted in the summers 2006 and 2007 on a Haplic Podzol under a 140 years old Norway spruce stand using a roof installation followed by re-wetting compared to non-manipulated control plots. Total organic carbon, lignin (stable carbon pool), plant and microbial sugars (labile carbon pool) and microbial biomass (phospholipid fatty acids) were determined before, during and after the experiment in the L, O, A and B horizons. No significant treatment effects could be observed for SOM quantity. Amounts of lignin and soil microbial biomass were also not affected by the moisture regime but structure of soil microbial community. In the L and organic layers, gram + bacteria and actinomycetes were reduced during water stress, while gram- bacteria, fungi and protozoa increased during drought. Warmer and drier weather led to a dominance of fungi while a cooler and moister regime favoured bacteria, at least in the L horizon. An increasing PLFA (cy17:0 + cy19:0)/(16:1ω7c + 18:1ω7c) ratio in the O layer and A horizon suggests that the microbes suffered from water stress in these horizons. This agrees with a decreasing contribution of microbial sugars to SOM with decreasing water content in the O and A horizons. Although the original plant material exhibited increasing plant sugar content with increasing dryness, the contribution of the plant sugars to total soil organic carbon (SOC) generally decreased with decreasing water content. Physical-chemical changes of soil structure can theoretically change the sugar extractability from soils and/or chemical changes of sugars structure can probably affect the analysis. Therefore, chemical alteration and stabilization could be responsible for sugar decrease in soil with increasing dryness explaining the contrast compared to the original plant material.     

The effects of changes in soil moisture on nitrogen cycling in acid wetland types of the New Jersey Pinelands (USA)

Wetlands are subject to changes in soil moisture as a result of both short-term seasonal climate variations and long-term changes in regional water resource management, both of which can modify the dynamics of ground and surface water inputs. In the New Jersey Pinelands, forested wetlands that differ in both plant communities and soil structure occur along a topographic and hydrological gradient associated with an unconfined aquifer. Proposed groundwater withdrawals may affect water content of soils along this gradient. We hypothesized that prolonged changes in soil moisture would alter net nitrogen mineralization and nitrification rates in proportion to the amount of moisture change, and that these changes would be similar for the different soils along the drainage catena. Soils from two catenary sequences of wetlands, including pine-dominated (driest landscape position), hardwood-dominated, and Atlantic white-cedar-dominated (wettest landscape position) communities were used in long-term laboratory incubations (36 weeks). Production of NH₄⁺-N, NO₃⁻-N, and dissolved organic N were measured under two sets of conditions: constant moisture levels of 100%, 60% and 30% water-holding capacity (WHC), and fluctuating moisture levels (alternating 2 week periods at 100% and 30% WHC). In soils from most of the wetlands, we observed increases in net mineralization and nitrification when constant low-moisture conditions were established, but not under fluctuating conditions. Contrary to expectations, responses to the drying treatments varied between wetland types and between replicate wetlands of each type. Under constant-moisture conditions, nitrification increased more in cedar swamps than in either type of pine wetland. Under all conditions, soils from all the wetlands within one of the catenas produced more inorganic and organic soluble N than did the wetlands from the other catena, suggesting that area-wide effects are as important as wetland type in regulating production of soluble N. Within both catenas, pine-hardwood wetlands generated more soluble N under all moisture conditions than did either pine-dominated or cedar wetlands. Our results suggest that changes in soil moisture due to management of water resources will affect N cycling in wetland soils, but that the magnitude of the effects, and the potential for large releases of nitrate, will depend on the specific soil properties of affected wetlands.     

Impact of irrigation water quality on soil nitrifying and total bacterial communities

Disturbance induced by two contrasting irrigation regimes (groundwater versus urban wastewater) was evaluated on a sandy agricultural soil through chemical and microbial analyses. Contrary to wastewater, groundwater displayed very high nitrate contents but small amounts of ammonium and organic matter. Despite these strong compositional shifts, soil organic carbon and nitrogen, nitrate and ammonium contents were not significantly different in both types of irrigated plot. Moreover, neither microbial biomass nor its activity, determined as fluorescein diacetate hydrolysis activity, was influenced by irrigation regimes. Bacterial community structure, assessed by denaturing gradient gel electrophoresis (DGGE) of 16S ribosomal DNA fragments, was also weakly impacted as molecular fingerprints shared an overall similarity of 85%. Ammonia-oxidizing bacterial community (AOB) was monitored by DGGE of the functional molecular marker amoA gene (alpha subunit of the ammonia monooxygenase). Surprisingly, no amoA signals were obtained from plots irrigated with groundwater, whereas signal intensities were high in all plots under wastewater. Among the last, compositional shifts of the AOB community were weak. Overall, impact of irrigation water quality on soil chemistry could not be evidenced, whereas effects were low on the total bacterial compartment but marked on the AOB community.     

黄土高原森林带土壤养分和微生物量及其生态化学计量变化特征

选择黄土高原森林带自东向西8个地区优势植物的土壤作为研究对象,探究不同经度下土壤养分和土壤微生物生物量生态化学计量特征。结果表明:土壤碳、氮和微生物生物量碳、氮、磷含量随经度整体呈现出先减少后增加的变化趋势,土壤微生物生物量碳氮磷与土壤碳氮之间存在耦合关系,对环境因子的响应具有一致性。土壤磷含量空间分布比较稳定。土壤碳氮比为8左右,土壤有机碳与全氮的空间分布具有一致性。土壤微生物生物量碳氮比为9左右,微生物生物量氮磷比为5左右,反映了土壤微生物生物量碳氮、氮磷的比值较稳定,但土壤微生物生物量碳磷比呈现出先增大后减小的变化趋势。土壤养分及土壤微生物量碳氮磷与土壤水分含量相关性较强。土壤养分与土壤微生物量碳氮磷及其生态化学计量是环境因子综合作用的结果。     

Short-term dynamics of carbon and nitrogen after tillage in a freshwater marsh of northeast China

The Sanjiang Plain, one of the largest freshwater marshes in China, has experienced intensive cultivation over the past 50 years. However, there were few reports of short-term dynamics of soil carbon and nitrogen and CO₂ emission after tillage. In this paper, we studied the short-term dynamics of carbon and nitrogen after tillage in a freshwater marsh of northeast China. The results showed that response of carbon and nitrogen dynamic to tillage was different for intact wetland and soil cultivated for 10 years. Tillage was followed by immediate and significant increases in CO₂ efflux, which peaked at 0.25 h after tillage, four times higher than control in the wetland soils; while, only 2.5 times higher than control in the cultivated soils. Although, dissolved organic C (DOC) increased, the relative stability of microbial biomass C (MBC) pools together with the decreased respiration in the wetland soil suggested that the tillage did not lead to a burst in microbial activity and growth. Other factors such as moisture content before and after tillage may play an important role in determining microbial activity in the intact wetland. On the contrary, although dissolved organic C did not change, MBC pools, and soil respiration increase after tillage, suggesting tillage led to an increase in microbial activity and growth in the cultivated soil. Tillage initiated changes in soil aeration that was an important factor affecting soil microbiology in the long history of cultivation. Net N mineralization and nitrification occurred in both wetland and cultivated soils, but at different rates after tillage that in the intact wetland soil was higher than cultivated soil. Macroaggregates in the wetland soil would be expected to contain larger amounts of organic matter, and thus release a larger source of newly available substrate for microbes after tillage. In the intact wetland soil, ammonium, nitrate, and dissolved organic N (DON) concentrations were significantly negatively correlated to soil moisture (p < 0.01), suggesting high soil moisture in the natural wetland was not in favor of N mineralization.     

黄土区夏闲期土壤呼吸变化特征及其影响因素

本文以1984年设立在黄土旱塬区长期田间定位试验为平台,于2009年采用动态密闭气室法(Li-8100,USA),监测了不同施肥措施下旱地冬麦种植系统中休闲期(7月至9月)土壤呼吸、10cm土层的温度和含水量变化,研究了休闲期土壤呼吸变化特征及其与环境因子的关系。结果表明:黄土旱塬农田休闲期土壤呼吸速率变化剧烈,最大值为5.05μmol m-2s-1,最小值为0.06μmol m-2s-1,平均值为2.00μmol m-2s-1,变异系数为116.5%;整个休闲期不同施肥处理的土壤呼吸速率大小为:化肥有机肥配施处理(NMP)有机肥处理(M)化肥氮磷处理(NP)化肥氮处理(N)和不施肥处理(CK);2009年7月7日至9月11日间NPM、M、NP、N和CK处理土壤CO2-C排放量分别为2.0、1.6、1.2、0.8和0.8 Mg hm-2;土壤呼吸与土壤水分为极显著抛物线关系(p0.01),可解释55%以上的土壤呼吸变异性;土壤呼吸与土壤温度呈显著线性相关(p0.01),但仅能解释呼吸作用变异性的19%~39%;土壤呼吸对耕作的响应强度与微生物量碳极显著线性正相关(p0.01),与土壤有机碳显著线性正相关(p0.05),与全氮、可溶性碳无明显关系(p0.05);降雨对土壤呼吸的促进或抑制主要取决于降雨前的土壤水分状况。长期水分亏缺降雨,降雨可明显促进土壤呼吸,而土壤水分充足时,降雨抑制土壤呼吸,其影响大小与土壤有机碳、全氮、土壤可溶性碳和微生物量碳密切相关。休闲期土壤呼吸受土壤水分、土壤干湿变化、土壤温度、翻耕及土壤有机碳水平等因素的影响。