基于分层抽样的中国水稻种植面积遥感调查方法研究

及时准确的统计水稻种植面积对国家和区域的粮食生产、贸易及粮食安全预警有重要意义。传统的按行政单元逐级上报和农业产量抽样调查方法在数据获取过程中受人为因素的干扰，难以避免的出现诸如错报、漏报、空报等问题。遥感技术具有及时、准确、客观的特点，对于农作物种植面积监测具有其他方法不可替代的优势。但是，一般的作物面积遥感监测是全覆盖或典型地区调查。在大尺度农作物遥感调查时，全面普查(卫星遥感数据全覆盖)的方法在时间和经费方面是不可行的，以典型调查代替总体的方法缺乏科学依据。科学合理的抽样方法是可运行的大尺度作物面积监测的关键因素。研究在背景数据库的支持下，以土地利用数据库为辅助变量，设计了基于分层抽样的中国水稻种植面积遥感监测方法。以全国稻田面积为总体，采用1∶5万比例尺标准地形图幅为分层抽样的抽样单元。以遥感与地面调查相结合的方法监测样本的当年和上一年水稻种植面积，在给定精度条件下估算水稻种植面积年际变化率。结合上年统计部门发布的水稻种植面积统计数据，推算当年水稻种植面积。该项研究为农业部全国水稻遥感监测提供了可行的大尺度水稻遥感监测的运行方案。     

基于CBERS-1卫星图像的新疆棉花遥感监测技术体系

新疆是我国重要的产棉区。全面、快速、客观地监测棉花种植面积的年际波动情况，可为生产管理部门提供重要的决策依据。该项目是CBRES-1应用示范项目之一，目的是研究其应用潜力。该研究建立了基于CBERS-1卫星图像的新疆棉花种植面积遥感监测运行系统的技术体系，实现了新疆棉花种植面积的遥感监测，并为应用中巴资源一号卫星CCD数据监测我国其它大宗农作物的种植面积提供示范，为后续卫星在农业领域的大规模应用打下基础。     

作物种植面积空间对地抽样方法设计

传统的粮食作物种植面积估算一般采用目录抽样方法，由于缺乏现实、有效的先验知识，抽样过程受精度和效率制约。本文利用地理信息系统、遥感和全球定位系统技术，结合传统的随机、系统和分层抽样方法，设计三种新的粮食作物种植面积空间对地抽样方案，并在此基础上开展试验对比研究，分析它们在抽样精度、最少样本量和稳定性方面的差异。结果表明，本文所设计的空间分层抽样方法所需样本量较小，且具有较高的估算精度和稳定性，可以用于大范围农作物种植面积监测。     

基于Landsat TM图像棉花面积提取中线状地物的扣除方法研究

在新疆棉花遥感监测中，发现各种线状地物对TM影像图中的棉花面积提取精度产生影响，直接对图像进行处理和分类这种影响难以消除。经实际调查，新疆某一农场的各种线状地物占到总耕地面积的12．8％。为提高作物面积提取精度，完善农业部全国棉花遥感监测运行系统，进行了线状地物扣除方法的试验研究。阐述了试验研究方法的理论依据、技术路线与实施过程。并经验证可消除绝大部分线状地物对作物面积提取精度产生的影响。     

小地物比例提取在河北耕地遥感调查中的应用研究

小地物的存在是影响大尺度下遥感面积监测的重要因素。该文以河北省为研究区域,借鉴分层抽样的基本思想,采用分区抽样方法,在不同立地条件的耕地上布设小地物抽样样方,共99个,最终得到河北省耕地中小地物面积比例的一系列估计值。其中,水田为10.15％,平原为3.51％,丘陵为7.14％。利用该数据对河北耕地面积遥感调查结果进行修正,可有效提高其结果的精度,对于河北省耕地面积遥感调查、作物面积遥感调查等应用具有实用性和使用价值,同时对具有相似立地条件的地区有重要的参考价值。     

全国冬小麦面积变化遥感监测抽样外推方法的研究

该文提出了一种可以用于业务化运行的全国冬小麦面积变化遥感监测方法。根据我国冬小麦生产的特点,采用分层抽样方法,以全国冬小麦种植面积以往的统计数据为分层指标,将全国1411个冬小麦生产县划分为6层,建立全国冬小麦面积变化遥感监测抽样外推模型;然后,以TM影像覆盖来近似随机地从各层抽取所需数量的冬小麦生产县,采用人工判读的方法解译冬小麦的变化,并以县为单位统计;最后利用外推模型得出全国冬小麦面积的变化。通过1999、2000年连续2年的运行试验,得到了满意的结果     

大尺度作物面积遥感监测中小地物的影响与双重抽样

在大尺度作物面积遥感监测中，一般采用抽样方法。由于小地物在影像上无法识别或难以量算，形成样本误差而影响总体面积的估计。改进的措施是采用双重抽样的方法，即在抽样设计中，首先控制作物样本精度，从而消除或减小小地物影响，进而获得较准确的作物面积绝对值。     

基于结构规模的冬小麦种植面积遥感抽样估算

在种植结构复杂地区,由于受到混合像元和同期作物的影响,传统的以规模为分层标志进行冬小麦种植面积遥感估算难以保证抽样效率和精度。该文综合考虑混合像元、同期作物的影响,构建了结构规模指标进行冬小麦种植面积遥感抽样估算。采用TM和QuickBird为研究数据,设计不同的抽样方案估算冬小麦的种植面积,计算标准误差、准确度和变异系数衡量估算精度,与传统简单随机、规模指标分层抽样进行对比分析,验证本文方法的有效性。试验结果表明,以结构规模指标分层抽样的反推结果在各项指标上均明显优于传统简单随机、规模指标分层抽样方式,尤其在小样本量时,标准误差降低2.0×105m2,准确度提升了1%。该研究结果为在大范围种植结构复杂地区进行冬小麦种植面积遥感估算的改进提供了试验依据。     

“3S”为基础的面积框对地抽样调查技术在农业调查中的应用

综合国内外农业遥感领域对地抽样调查技术,详细说明目前世界上主要采用3S技术（RS、GIS、GPS）的面积框抽样技术,以及主要代表国家的应用效果。描述了分层抽样统计学原理和主要采用的抽样方法。归纳了国内农业对地抽样技术的发展水平和存在的三个主要问题：一是侧重于农业遥感监测技术方法的研究,遥感抽样调查研究偏弱;二是遥感抽样调查研究科学性不足,多采用随意抽样或典型抽样,偏离了抽样调查的随机性特点;三是遥感技术与传统统计抽样方法结合不够。     

空间自相关性对冬小麦种植面积空间抽样效率的影响

空间抽样是实现区域农作物面积高效估算的重要手段,农作物分布受自然条件等因素影响普遍存在空间自相关性,但以往针对空间相关性对农作物面积抽样效率的影响研究明显不足。该研究选取安徽省凤台县为研究区,通过2017年4月4景GF-1全色多光谱影像(Panchromatic and Multispectral, PMS)与Google Earth高空间分辨率影像相结合提取研究区冬小麦。设计10种抽样单元尺度、3种抽样外推方法、2种相对允许误差和5种样本布局方式,构建多种冬小麦面积空间抽样方案。利用全局莫兰指数(global Moran’s index)评价不种尺度下抽样单元内冬小麦面积比的空间自相关强度,分析空间自相关性对冬小麦面积抽样效率(抽样误差、样本容量和空间布局)的影响。研究结果表明,抽样单元内冬小麦面积比的空间自相关强度随单元尺度的增大而减小,全局莫兰指数相应地由0.75降至0.50。无论在何种尺度下抽样单元内冬小麦面积比都呈显著的空间正相关性;抽样外推冬小麦面积总体的误差随空间自相关强度的减小呈先减小后明显增大的趋势。在10种抽样单元尺度中,当抽样单元尺度为2000m且抽样比为5%时,无论采用何种抽样方法外推总体的误差均为最小(简单随机抽样、系统和分层抽样外推总体的相对误差分别为17.94%、9.48%和1.82%);当相对允许误差设计为5%时,简单随机抽样外推总体所需样本容量随空间自相关强度的降低从660降至56。而分层抽样的样本容量不受空间自相关性的影响;5种样本布局方式中,采用分层随机抽样方式外推冬小麦面积总体的平均相对误差、平均变异系数和均方根误差最小,分别为1.82%、3.19%和0.11×108 m2。该研究可为有空间自相关存在下的农作物面积空间抽样方案合理设计提供参考依据。     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.     

Variation in quality parameters between and within 14 Nordic tree fruit and berry species

Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.     

Potassium Fixation and Release Characteristics in Rhizosphere and Nonrhizosphere Soils for a Rapeseed–Rice Cropping Sequence

Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L^?1 nitric acid (HNO₃) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L^?1 ammonium acetate (NH₄OAc) and 1.0 mol L^?1 HNO₃ extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.     

Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater

Biologically enhanced dissolution offers a method to speed removal of chlorinated solvent dense non-aqueous-phase liquid (DNAPL) sources such as tetrachloroethene (PCE) and trichoroethene (TCE) from aquifers. Bioremediation is accomplished by adding an electron donor to the source zone where fermentation to intermediates leading to acetic acid and hydrogen results. The hydrogen and possibly acetic acid are used by dehalogenating bacteria to convert PCE and TCE to ethene and hydrochloric acid. Reductive dehalogenation is thus an acid forming process, and sufficient alkalinity must be present to maintain a near neutral pH. The bicarbonate alkalinity required to maintain pH above 6.5 is a function of the electron donor: 800 mg/L of bicarbonate alkalinity is sufficient to achieve about 1.2 mM TCE dechlorination with glucose, 1.7 mM with lactate, and a much higher 3.3 mM with formate. Laboratory studies indicate that in mixed culture, formate can be used as an electron donor for complete conversion to ethene, contrary to pure cultures studies indicating it cannot. Various strategies can be used to add electron donor to an aquifer for DNAPL dehalogenation while minimizing pH problems and excessive electron donor usage, including use of injection-extraction wells, dual recirculation wells, and nested injection-extraction wells.     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense

Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene. This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots. Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production or the positive response of the plants towards A. brasilense Cd inoculation.     

In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous beads

We examined the community composition of microbes that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment history. Bacterial abundance was 5-40-fold greater in atrazine-fortified beads. In beads containing 20 mg atrazine kg⁻¹ buried in soil with a history of atrazine application (conditioned soil), the abundance of Actinobacteria increased approximately 80-fold whereas in control soil, Actinobacteria were enriched only 10-fold and the gamma-Proteobacteria and Planctomycetes increased by 60- and 25-fold, respectively. The gamma-Proteobacteria were enriched by 120- and 230-fold in beads containing 200 mg atrazine kg⁻¹ in conditioned and control soil, respectively. The results demonstrate that BioSep^® beads are a suitable matrix for recruiting a diverse subset of the bacterial community involved in atrazine degradation.