Vulnerability of lodging risk to elevated CO2 and increased soil temperature differs between rice cultivars

Anthropogenic increases in atmospheric carbon dioxide concentration [CO₂], and subsequent increases in surface temperatures, are likely to impact the growth and yield of cereal crops. One potential means for yield reduction is for climate parameters to increase the occurrence of lodging. Using an in situ free-air CO₂ enrichment (FACE) system, two morphologically distinct rice cultivars, KH (Koshihikari) and SY (Shan you 63), were grown at two [CO₂]s (ambient and ambient + 200 μmol mol⁻¹) and two soil temperatures (ambient and ambient ± 1.8 °C) over a two year period to assess and quantify lodging risk. Elevated [CO₂] per se had no effect on lodging resistance for either cultivar. However, elevated [CO₂] and higher soil temperature increased the lodging risk for SY, due to a relatively higher increase in plant biomass and height at the elevated, relative to the ambient [CO₂] condition. Elevated soil temperature per se also increased lodging risk for both cultivars and was associated with longer internodes in the lower portion of the tillers. These findings illustrate that lodging susceptibility in rice, an important cereal crop, can be increased by rising [CO₂] and soil temperature; however, variation observed here between rice cultivars suggests there may be sufficient intraspecific variability to begin choosing rice lines that minimize the potential risk of lodging.     

Trade-offs between water-use related traits,yield components and mineral nutrition of wheat under Free-Air CO2 Enrichment (FACE)

This study investigated trade-offs between parameters determining water use efficiency of wheat under elevated CO₂ in contrasting growing seasons and a semi-arid environment. We also evaluated whether previously reported negative relationships between nutrient content and transpiration efficiency among wheat genotypes will be maintained under elevated CO₂ conditions. Two cultivars of wheat (Triticum aestivum L.), Scout and Yitpi, purportedly differing in water use efficiency related traits (e.g. transpiration efficiency) but with common genetic backgrounds were studied in a high yielding, high rainfall (2013), and in a low yielding, very dry growing season (2014) under Free-Air CO₂ Enrichment (FACE, CO₂ concentration of approximately 550 μmol mol⁻¹) and ambient (approximately 390 μmol mol⁻¹) CO₂. Gas exchange measurements were collected diurnally between stem elongation and anthesis. Aboveground biomass and nutrient content (sum of Ca, K, S, P, Cu, Fe, Zn, Mn and Mg) were determined at anthesis. Yield, yield components and harvest index were measured at physiological maturity. Cultivar Scout showed transiently greater transpiration efficiency (measured by gas exchange) over cultivar Yitpi under both ambient and elevated CO₂ conditions, mainly expressed in the high yielding but not in the low yielding season. Nutrient content was on average 13% greater for the lower transpiration efficiency cultivar Yitpi than the cultivar with higher transpiration efficiency (Scout) in the high yielding season across both CO₂ concentrations. Elevated CO₂ stimulated grain yield to a greater extent in the high yielding season than in the low yielding season where increased aboveground biomass earlier in the season did not translate into fertile tillers in cultivar Yitpi. Yield increased 27 and 33% in the high yielding and 0 and 19% in the low yielding season for cultivars Yitpi and Scout, respectively. Intraspecific variation in CO₂ responsiveness related mechanisms of grain yield were observed. These results suggest CO₂-driven trade-offs between traits governing water use efficiency are related to both growing season and intraspecific variations, and under very dry finishes, the trade-offs may even reverse. The negative relationship between nutrient content and transpiration efficiency among wheat genotypes will be maintained under elevated CO₂ conditions.     

Atmospheric CO2 enrichment affects potatoes: 1. Aboveground biomass production and tuber yield

Despite its economic and environmental importance, information about effects of future atmospheric carbon dioxide (CO₂) enrichment on aboveground biomass production and tuber yield of potato is still rare. Responses of potato (Solanum tuberosum L. cv. Bintje) were thus investigated in two full growing seasons under 380, 550 or 680 μmol mol^?1 CO₂ in open-top chambers (OTCs). When averaged over both years, aboveground stem biomass at canopy maturity was negatively related to CO₂ enrichment. Aboveground-to-belowground biomass ratio was negatively related to CO₂ enrichment as there was a positive relationship between CO₂ and total dry yield of potato tubers. The stimulation was mainly related to an increase in the tuber size fraction for commercial yield (tubers > 35 mm). For the largest size class (tubers > 50 mm), which is important for industrial processing, large CO₂-induced impacts were observed too, although these effects were not significant. Elevated CO₂ concentrations will thus affect biomass allocation of potato plants and result in improvements concerning the market value of the commercial tuber yield.     

Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit

Tomato fruit (Lycopersicon esculentum L. cv. Carousel) were exposed to ozone concentrations ranging between 0.005 (controls) and 1.0 μmol mol⁻¹ at 13 °C and 95% RH. Quality-related attributes and organoleptic characteristics were examined during and following ozone treatment. Levels of soluble sugars (glucose, fructose) were maintained in ozone-treated fruit following transfer to ‘clean air’, and a transient increase in β-carotene, lutein and lycopene content was observed in ozone-treated fruit, though the effect was not sustained. Ozone-enrichment also maintained fruit firmness in comparison with fruit stored in ‘clean air’. Ozone-treatment did not affect fruit weight loss, antioxidant status, CO₂/H₂O exchange, ethylene production or organic acid, vitamin C (pulp and seed) and total phenolic content. Panel trials (employing choice tests, based on both appearance and sensory evaluation) revealed an overwhelming preference for fruit subject to low-level ozone-enrichment (0.15 μmol mol⁻¹), with the effect persisting following packaging.     

Assessing the impact of increasing carbon dioxide and temperature on crop-weed interactions for tomato and a C3 and C4 weed species

Based on the carboxylation kinetics of the C₃ and C₄ photosynthetic pathway, it is anticipated that C₃ crops may be favored over C₄ weeds as atmospheric CO₂ increases. In the current study, tomato (Lycopersicon esculentum), a C₃ crop species, was grown at ambient (~400 μmol mol⁻¹) and enhanced carbon dioxide (~800 μmol mol⁻¹) with and without two common weeds, lambsquarters (Chenopodium album), a C₃ weed, and redroot pigweed (Amaranthus retroflexus), a C₄ weed, from seedling emergence until mutual shading of crop-weed leaves. Because growth temperature is also likely to change in concert with rising CO₂, the experiment was repeated at day/night temperatures of 21/12 and 26/18 °C. For both day/night temperatures, elevated CO₂ exacerbated weed competition from both the C₃ and C₄ weed species. A model based on relative leaf area following emergence was used to calculate potential crop losses from weeds. This analysis indicated that potential crop losses increased from 33 to 55% and from 32 to 61% at the 21/12 and 26/18 °C day/night temperatures, for ambient and elevated CO₂, respectively. For the current study, reductions in biomass and projected yield of tomato appeared independent of the photosynthetic pathway of the competing weed species. This may be due to inherent variation and overlap in the growth response of C₃ and C₄ species, whether weeds or crops, to increasing CO₂ concentration. Overall, these results suggest that as atmospheric CO₂ and/or temperature increases, other biological interactions, in addition to photosynthetic pathway, deserve additional consideration in predicting competitive outcomes between weeds and crops.     

Field experiments and simulation to evaluate rice cultivar adaptation to elevated carbon dioxide and temperature in sub-tropical India

Location specific adaptation option is required to minimize adverse impact of climate change on rice production. In the present investigation, we calibrated genotype coefficients of four cultivars in the CERES-Rice model for simulation of rice yield under elevated CO₂ environment and evaluation of the cultivar adaptation in subtropical India. The four cultivars (IR 36, Swarna, Swarn sub1, and Badshabhog) were grown in open field and in Open Top Chamber (OTC) of ambient CO₂ (≈390 ppm) and elevated CO₂ environment (25% higher than the ambient) during wet season (June–November) of the years 2011 and 2012 at Kharagpur, India. The genotype coefficients; P1 (basic vegetative phase), P2R (photoperiod sensitivity) and P5 (grain filling phase) were higher, but G1 (potential spikelet number) was lower under the elevated CO₂ environment as compared to their open field value in all the four cultivars. Use of the calibrated model of elevated CO₂ environment simulated the changes in grain yield of −13%, −17%, −4%, and +7% for the cultivars IR 36, Swarna, Swarna sub1, and Badshabhog, respectively, with increasing CO₂ level of 100 ppm and rising temperature of 1 °C as compared to the ambient CO₂ level and temperature and they were comparable with observed yield changes from the OTC experiment. Potential impacts of climate change were simulated for climate change scenarios developed from HadCM3 global climate model under the Intergovernmental Panel on Climate Change Special Report on Emission Scenarios (A2 and B2) for the years 2020, 2050, and 2080. Use of the future climate data simulated a continuous decline in rice grain yield from present years to the years 2020, 2050 and 2080 for the cultivars IR 36 and Swarna in A2 as well as B2 scenario with rising temperature of ≥0.8 °C. Whereas, the cultivar Swarna sub1 was least affected and Badshabhog was favoured under elevated CO₂ with rising temperature up to 2 °C in the sub-tropical climate of India.     

Prestorage application of high carbon dioxide combined with controlled atmosphere storage as a dual approach to control Botrytis cinerea in organic ‘Flame Seedless’ and ‘Crimson Seedless’ table grapes

Pre-storage application of 40% CO₂ at 0 °C for 24 or 48 h and controlled atmosphere (12% O₂ + 12% CO₂) storage at 0 °C for up to eight weeks on decay control and quality of organic ‘Flame Seedless’ and ‘Crimson Seedless’ table grapes were studied as a postharvest disease control alternative. To simulate different potential field conditions, these organic treatments were applied to organic-grown grapes that were naturally infected (without inoculation), surface inoculated (berries inoculated by spraying with a conidia suspension), and nesting inoculated (clusters inoculated by placing in the middle an artificially infected berry) with the pathogen Botrytis cinerea, the cause of grape gray mold. Under these three conditions, a 40% CO₂ for 48 h pre-storage treatment followed by controlled atmosphere reduced the gray mold incidence from 22% to 0.6% and from 100% to 7.4% after four and seven weeks, respectively. High CO₂ pre-storage alone limited botrytis incidence in both naturally and artificially infected grapes, but was more effective when combined with CA. These treatments did not affect visual or sensory fruit quality. Exposure to high CO₂ for 24 or 48 h effectively inhibited mycelial growth of B. cinerea in PDA plates incubated at 22 °C for up to 72 h. Conidia germination in PDA plates was reduced ∼60% after 12 h incubation. In vitro studies demonstrated a fungistatic effect, but further studies on the mechanism of action could improve treatment performance. This novel high CO₂ initial fumigation followed by controlled atmosphere during storage or transportation could be a commercially feasible alternative for postharvest handling of organic and conventional table grapes. Our results encourage validating this combined physical treatment in other cultivars and under commercial conditions.     

Effects of irrigation and nitrogen fertilization on the greenhouse gas emissions of a cropping system on a sandy soil in northeast Germany

Irrigation induces processes that may either decrease or increase greenhouse gas emissions from cropping systems. To estimate the net effect of irrigation on the greenhouse gas emissions, it is necessary to consider changes in the crop yields, the content of soil organic carbon and nitrous oxide emissions, as well as in emissions from the use and production of machinery and auxiliary materials. In this study the net greenhouse gas emissions of a cropping system on a sandy soil in northeast Germany were calculated based on a long-term field experiment coupled with two-year N₂O flux measurements on selected plots. The cropping system comprised a rotation of potato, winter wheat, winter oil seed rape, winter rye and cocksfoot each under three nitrogen (N) fertilization intensities with and without irrigation. Total greenhouse gas emissions ranged from 452 to 3503 kg CO_2-eq ha⁻¹ and 0.09 to 1.81 kg CO_2-eq kg⁻¹ yield. Application of an adequate amount of N fertilizer led to a decrease in greenhouse gas emissions compared to zero N fertilization whereas excessive N fertilization did not result in a further decrease. Under N fertilization there were no significant differences between irrigation and non-irrigation. Increases in greenhouse gas emissions from the operation, production and maintenance of irrigation equipment were mainly offset by increases in crop yield and soil organic carbon contents. Thus, on a sandy soil under climatic conditions of north-east Germany it is possible to produce higher yields under irrigation without an increase in the yield-related greenhouse gas emissions.     

High CO2 effects on postharvest biochemical and textural properties of white asparagus (Asparagus officinalis L.) spears

The effects of high CO₂ concentration (10% CO₂, 17% O₂) on the changes of functional cell wall components (pectic substances, hemicellulose, cellulose, lignin), mechanical properties, content of free soluble sugars (sucrose, glucose, fructose), and respiration activity were studied in harvested white asparagus spears stored at 10 and 20 °C, respectively, for up to 7 d. Spears stored at 2, 10 and 20 °C in air were studied as controls, where the 2 °C condition indicated the effects of cold storage. During storage, respiration activity declined only slightly, irrespective of the CO₂ and temperature regime. Spears stored at 20 °C under both CA and normal air became less stiff and more elastic, however, tissue toughness increased significantly. Changes in toughness were associated primarily with the dynamics of lignin and cellulose, revealing a strong correlation (r² = 0.81). High CO₂ concentration inhibited the synthesis of cellulose and, to some extent, lignin accumulation at 20 °C. Additionally, elevated CO₂ inhibited the degradation of soluble carbohydrates. In contrast, slightly lower temperatures of 10 °C in combination with high CO₂ did not have a pronounced effect on changes in structural carbohydrates (lignin, cellulose, hemicellulose and pectins). The effect low temperature (2 °C) under normal atmosphere conditions resulted in the inhibition of cell wall changes in asparagus spears.     

Exploring options for managing strategies for pea–barley intercropping using a modeling approach

Although quality of potato tubers is an important topic with regard to food and industrial processing, the consequences of future atmospheric carbon dioxide (CO₂) enrichment on related attributes are still unclear. Effects of elevated CO₂ concentrations on yield quality of potato (Solanum tuberosum L. cv. Bintje) were thus investigated in two full growing seasons under 380, 550 or 680 μmol mol^?1 CO₂ in open-top chambers (OTCs). When averaged over both years, tuber malformation was increased by 62.8% as CO₂ levels raised from 380 to 550 μmol mol^?1, resulting in a negative impact on tuber quality. In contrast, elevated CO₂ caused lower tuber greening and therefore enhanced tuber market value. Physical potato quality remained largely unchanged, except for the positive relationships between CO₂ and dry matter content, resulting in higher tuber quality for industrial processing. Significant relationships were also observed between CO₂ concentrations and several chemical quality parameters. The concentrations of glucose, fructose and total reducing carbohydrates were positively related to CO₂ levels, which decrease tuber quality due to the higher risk for browning and generation of acrylamide of fried products. The concentrations of protein, potassium, and as a trend of calcium, were negatively related to CO₂ enrichment, suggesting adverse impacts on tuber quality for human nutrition and aesthetic and sensory quality during processing. Significant negative relationships between CO₂ treatments and concentrations of leucine, phenylalanine and methionine, and as a trend for di-tyrosine, histidine and aspartic acid, were also indicated, which may decrease nutrition quality of potatoes because of the reduction in physiologically valuable amino acids. With regard to organic acids, CO₂-related alterations were restricted to lower concentrations of citric acid. This may reduce the processing quality of potato tuber, as there is a higher risk of discoloration, and at the same time improve quality aspects related to taste. In addition, the experiments indicated negative relationships between CO₂ concentrations and total glycoalkaloids and α-chaconine, which may have negative effects on the taste of potato at low concentration of glycoalkaloids of the present study. Concomitantly, the CO₂-induced decrease in glykoalkaloids may be regarded as an improvement of quality in terms of lower toxicological potential. CO₂ enrichment may thus cause substantial impacts, both positive and negative, on tuber quality of potato with regard to commercial value, industrial processing and consumer nutrition and health in the future. Currently, no clear evidence exists whether CO₂-induced beneficial changes will outweigh adverse effects on tuber quality.     

Yield,growth and grain nitrogen response to elevated CO2 in six lentil (Lens culinaris) cultivars grown under Free Air CO2 Enrichment (FACE) in a semi-arid environment

Atmospheric CO₂ concentrations ([CO₂]) are predicted to increase from current levels of about 400 ppm to reach 550 ppm by 2050. The direct benefits of elevated [CO₂] (e[CO₂]) to plant growth appear to be greater under low rainfall conditions, but there are few field (Free Air CO₂ Enrichment or FACE) experimental set-ups that directly address semi-arid conditions. The objectives of this study were to investigate the following research questions: 1) What are the effects of e[CO₂] on the growth and grain yield of lentil (Lens culinaris) grown under semi-arid conditions under FACE? 2) Does e[CO₂] decrease grain nitrogen in lentil? and 3) Is there genotypic variability in the response to e[CO₂] in lentil cultivars? Elevated [CO₂] increased yields by approximately 0.5 t ha⁻¹ (relative increase ranging from 18 to 138%) by increasing both biomass accumulation (by 32%) and the harvest index (by up to 60%). However, the relative response of grain yield to e[CO₂] was not consistently greater under dry conditions and might depend on water availability post-flowering. Grain nitrogen concentration was significantly reduced by e[CO₂] under the conditions of this experiment. No differences were found between the cultivars selected in the response to elevated [CO₂] for grain yield or any other parameters observed despite well expressed genotypic variability in many traits of interest. Biomass accumulation from flowering to maturity was considerably increased by elevated [CO₂] (a 50% increase) which suggests that the indeterminate growth habit of lentils provides vegetative sinks in addition to reproductive sinks during the grain-filling period.     

Soybean (Glycine max (L.) Merr.) growth and development response to CO2 enrichment under different temperature regimes

The carbon dioxide (CO₂) concentration of the global atmosphere has increased during the last decades. This increase is expected to impact the diurnal variation in temperature as well as the occurrence of extreme temperatures. This potentially could affect crop production through changes in growth and development that will ultimately impact yield. The objective of this study was to evaluate the effect of CO₂ and its interaction with temperature on growth and development of soybean (Glycine max (L.) Merr., cv. Stonewall). The experiment was conducted in controlled environment chambers at the Georgia Envirotron under three different temperatures and two CO₂ regimes. The day/night air temperatures were maintained at 20/15, 25/20 and 30/25 °C, while the CO₂ levels were maintained at 400 and 700 ppm, resulting in six different treatments. Plants were grown under a constant irradiance of 850 μmoles m⁻² s⁻¹ and a day length of 12 h; a non-limiting supply of water and mineral nutrients were provided. Five growth analyses were conducted at the critical development stages V4, R3, R5, R6 and R8. No differences in start of flowering were observed as a function of the CO₂ level, except for the temperature regime 25/20 °C, where flowering for the elevated CO₂ level occurred 2 days earlier than for the ambient CO₂ level. For aboveground biomass, an increase in the CO₂ level caused a more vigorous growth at lower temperatures. An increase in temperature also decreased seed weight, mainly due to a reduction in seed size. For all temperature combinations, final seed weight was higher for the elevated CO₂ level. This study showed that controlled environment chambers can be excellent facilities for conducting a detailed growth analysis to study the impact on the interactive effect of changes in temperature and CO₂ on soybean growth and final yield.     

Edible coatings containing chitosan and moderate modified atmospheres maintain quality and enhance phytochemicals of carrot sticks

Carrot sticks are increasingly in demand as ready-to-eat products, with a major quality problem in the development of white discoloration. Modified atmosphere packaging (MAP) and edible coating have been proposed as postharvest treatments to maintain quality and prolong shelf-life. The combined application of an edible coating containing 5 mL L^?1 of chitosan under two different MAP conditions (10 kPa O₂ + 10 kPa CO₂ in Pack A and 2 kPa O₂ + 15–25 kPa CO₂ in Pack B) over 12 d at 4 °C was studied. Respiration rate, microbial and sensory qualities as well as the contents of vitamin C, carotenoids and phenolics of coated and uncoated carrot sticks were evaluated. The use of the edible coating containing chitosan preserved the overall visual quality and reduced surface whiteness during storage. Microbial populations were very low and not influenced by coating or MAP. Edible coating increased respiration rates of carrot sticks, although this was only noticeable in the package with the less permeable film (Pack B). Vitamin C and carotenoids decreased during storage particularly in coated carrot sticks. In contrast, the content of total phenolics markedly increased in coated carrot sticks stored under moderate O₂ and CO₂ levels, while it was controlled under low O₂ and high CO₂ levels. The combined application of edible coating containing chitosan and moderate O₂ and CO₂ levels maintained quality and enhanced phenolic content in carrot sticks.     

Differential quenching of free chlorine by organic compounds potentially exuded from injured plant tissues

Fresh-cut fruits and vegetables can release significant amounts of metabolites from damaged tissues on cut edges. These metabolites can potentially quench oxidative sanitizers and hence lead to loss of their antimicrobial effectiveness. In this study, the effects of organic acids, carbohydrates, phenolics, other metabolites and hydrogen peroxide on depletion of chlorine were evaluated by quantitative monitoring chlorine loss in simulated wash solutions. Gallic acid, caffeic acid and most amino acids had the greatest capacities for depleting chlorine, requiring concentrations in the range of 10 μmol L⁻¹ or less to deplete free chlorine by half. Pyruvic, ascorbic, chlorogenic, malonic and oxalic acids had slight lower capacities, with concentrations ranging from 17 to 100 μmol L⁻¹ leading to half depletion. All nitrogen containing metabolites had relatively high capacity in depleting chlorine at concentrations in the range of 10 μmol L⁻¹, whereas hydrogen peroxide had a half depletion concentration of 21.3 μmol L⁻¹. In contrast, all sugars and most carboxylic acids had lower free chlorine depletion capacities. These results demonstrate that not all organic molecules potentially exuded from cut fruit or vegetable tissue had equal or similar potential to quench free chlorine from wash water.     

Effect of free air carbon dioxide enrichment combined with two nitrogen levels on growth,yield and yield quality of sugar beet: Evidence for a sink limitation of beet growth under elevated CO2

The increase in atmospheric CO₂ concentration [CO₂] has been demonstrated to stimulate the growth of C₃ crops. However, little information exists about the effect of elevated [CO₂] on biomass production of sugar beet, and data from field experiments are lacking. In this study, sugar beet was grown within a crop rotation over two rotation cycles (2001, 2004) at present and elevated [CO₂] (375 μl l^?1 and 550 μl l^?1) in a free air CO₂ enrichment (FACE) system and at two levels of nitrogen supply [high (N2), and 50% of high (N1)], in Braunschweig, Germany. The objective of the present study was to determine the CO₂ effect on seasonal changes of leaf growth and on final biomass and sugar yield. Shading treatment was included to test whether sugar beet growth is sink limited under elevated [CO₂]. CO₂ elevation did not affect leaf number but increased individual leaf size in early summer resulting in a faster row closure under both N levels. In late summer CO₂ enrichment increased the fraction of senescent leaves under high but not low N supply, which contributed to a negative CO₂ effect on leaf area index and canopy chlorophyll content under high N at final harvest. Petioles contained up to 40% water-soluble carbohydrates, which were hardly affected by CO₂ but increased by N supply. More N increased biomass production by 21% and 12% in 2001 and 2004, respectively, while beet and sugar yield was not influenced. Concentration of α-amino N in the beet fresh weight was increased under low N and decreased under high N by CO₂ enrichment. The CO₂ response of total biomass, beet yield and white sugar yield was unaffected by N supply. Averaged over both N levels elevated [CO₂] increased total biomass by 7% and 12% in 2001 and 2004, respectively, and white sugar yield by 12% and 13%. The shading treatment in 2004 prevented the decrease in leaf area index under elevated [CO₂] and high N in September. Moreover, the CO₂ effect on total biomass (24%) and white sugar yield (28%) was doubled as compared to the unshaded conditions. It is concluded that the growth of the storage root of sugar beet is not source but sink limited under elevated [CO₂], which minimizes the potential CO₂ effect on photosynthesis and beet yield.     

Controlled atmosphere storage of guava (Psidium guajava L.) fruit

The effects of controlled atmospheres (CA) on respiration, ethylene production, firmness, weight loss, quality, chilling injury, and decay incidence of three commercially important cultivars of guava fruit were studied during storage in atmospheres containing 2.5, 5, 8, and 10 kPa O₂ with 2.5, 5, and 10 kPa CO₂ (balance N₂) at 8 °C, a temperature normally inducing chilling injury. Mature light green fruit of cultivars, ‘Lucknow-49’, ‘Allahabad Safeda’ and ‘Apple Colour’, were stored for 30 days either in CA or normal air, and transferred to ambient conditions (25–28 °C and 60–70% R.H.) for ripening. CA storage delayed and suppressed respiratory and ethylene peaks during ripening. A greater suppression of respiration and ethylene production was observed in fruit stored in low O₂ (≤5 kPa) atmospheres compared to those stored in CA containing 8 or 10 kPa O₂ levels. High CO₂ (>5 kPa) was not beneficial, causing a reduction in ascorbic acid levels. CA storage was effective in reducing weight loss, and maintaining firmness of fruit. The changes in soluble solids content (SSC), titratable acidity (TA), ascorbic acid, and total phenols were retarded by CA, the extent of which was dependent upon cultivar and atmosphere composition. Higher amounts of fermentative metabolites, ethanol and acetaldehyde, accumulated in fruit held in atmospheres containing 2.5 kPa O₂. Chilling injury and decay incidence were reduced during ripening of fruit stored in optimal atmospheres compared to air-stored fruit. In conclusion, guava cultivars, ‘Lucknow-49’, ‘Allahabad Safeda’, and ‘Apple Colour’ may be stored for 30 days at low temperature (8 °C) supplemented with 5 kPa O₂ + 2.5 kPa CO₂, 5 kPa O₂ + 5 kPa CO₂, and 8 kPa O₂ + 5 kPa CO₂, respectively.     

Modelling soil tillage and mulching effects on soil water dynamics in raised-bed vegetable rotations

Reduced tillage and mulching may bring about new production systems that combine better soil structure with greater water use efficiency for vegetable crops grown in raised bed systems. These are especially relevant under conditions of high rainfall variability, limited access to irrigation and high soil erosion risk. Here we evaluate a novel combination of empirical models on water interception and infiltration, with a soil-water balance model to evaluate water dynamics in raised bed systems on fine Uruguayan soils to analyze the effect of reduced tillage, cover crops and organic matter addition on soil physical properties and water balance. In the experiment mulching increased water capture by 9.5% and reduced runoff by 37%, on average, leading to less erosion risk and greater plant available water over four years of trial. Using these data we calibrated and evaluated different models that predicted interception + infiltration efficiently (EF = 0.93 to 0.95), with a root mean squared error (RMSE) from 0.32 to 0.40 mm, for an average observed interception + infiltration of 28.8 mm per day per rainfall event. Combining the best model with a soil water balance resulted in predictions of total soil water content to 1 m depth (SWC_T) with RMSE ranging from 4.5 to 10.3 mm for observed SWC_T ranging from 180.4 to 380.6 mm. Running the model for a four-year crop sequence under 10 years of Uruguayan historical weather revealed that reduced tillage required on average 141 mm yr⁻¹ less irrigation water than conventional tillage combined with organic matter application, thus enabling a potential increase in irrigated area of vegetable crops and crop yields. Results also showed the importance of inter-annual rainfall variability, which caused up to 3-fold differences in irrigation requirements. The model is easily adaptable to other soil and weather conditions.     

Effect of non-conventional atmospheres and bio-based packaging on the quality and safety of Listeria monocytogenes-inoculated fresh-cut celery (Apium graveolens L.) during storage

The increased consumption of fresh-cut celery has led to the need to explore packaging alternatives for fresh-cut celery that can meet consumer, market, and industry needs. In this study, the effect of bio-based packaging and non-conventional atmospheres on the quality and safety of chlorine-sanitized celery sticks stored at 7 °C was investigated. Two materials differing in permeability [a bio-based polyester (polylactic acid (PLA)) and a petroleum-based polyolefin (polypropylene/low density polyethylene (PP/PE)] and four initial gas compositions [air (A-PLA or A-PP/PE), 95 kPa O₂ + 5 kPa N₂ (O₂-PLA), 99 kPa N₂ + 1 kPa O₂ (N₂-PLA), and 6 kPa O₂ + 12 kPa CO₂ + 82 kPa N₂ (CO₂-PLA)] were evaluated. Changes in headspace composition, weight loss, surface and cut end color, texture, ethanol content, appearance, and growth of Listeria monocytogenes on inoculated celery sticks were assessed during 21 d of storage. Active MAP (CO₂-PLA) out-performed passive MAP (A-PLA) in maintaining celery stick quality but not safety. Conventional active MAP (CO₂-PLA) out-performed non-conventional active MAPs (O₂-PLA and N₂-PLA) in maintaining celery stick quality throughout storage, but O₂-PLA suppressed L. monocytogenes growth while CO₂-PLA promoted growth during the first 10 d of storage. PLA and PP/PE materials affected celery stick quality but not Listeria growth. This study shows that the initial gas composition and packaging material both impact the quality and safety of celery sticks. Overall, the combination PLA and 95 kPa O₂ proved most beneficial in maximizing both the safety and quality of celery sticks during one week of storage at 7 °C.     

The relevance of N fertilization for the amount of total greenhouse gas emissions in sugar beet cultivation

The agricultural sector is highly affected by climate change and it is a source of greenhouse gases. Therefore it is in charge to reduce emissions. For a development of reduction strategies, origins of emissions have to be known. On the example of sugar beet, this study identifies the main sources and gives an overview of the variety of production systems. With data from farm surveys, calculations of greenhouse gas (GHG) emissions in sugar beet cultivation in Germany are presented. Emissions due to the production and use of fertilizers and pesticides, emissions due to tillage as well as field emissions were taken into account. All emissions related to the growing of catch crops during fall before the cultivation of sugar beet were also included. The emissions are related to the yield to express intensity.The median of total GHG emissions of sugar beet cultivation in Germany for the years 2010–2012 amounted to 2626 equivalents of CO₂ (CO₂eq) kg ha⁻¹ year⁻¹ when applying mineral plus organic fertilizer and to 1782 kg ha⁻¹ when only organic fertilizer was applied. The CO₂eq emissions resulting from N fertilization exclusively were 2.5 times higher than those caused by diesel and further production factors. The absence of emissions for the production of organic fertilizers led to 12% less total CO₂eq emissions compared to the use of mineral fertilizer only. But by applying organic fertilizer only, there were more emissions via the use of diesel due to larger volumes transported (126 l diesel ha⁻¹ vs. 116 l ha⁻¹ by applying mineral fertilizer exclusively).As there exists no official agreement about calculating CO₂eq emissions in crop production yet, the authors conclude that there is still need for further research and development with the aim to improve crop cultivation and crop rotations concerning GHG emissions and the therewith related intensity.     

Modelling photosynthetic efficiency (α) for the light-response curve of cocksfoot leaves grown under temperate field conditions

Net photosynthetic rate was measured from the youngest fully expanded leaves of field grown cocksfoot (Dactylis glomerata L.) in open pastures and under trees in the Lincoln University silvopastoral experiment (New Zealand). The photosynthetic efficiency (α) and convexity (θ) of the light-response curve were derived from 209 fitted non-rectangular hyperbola functions. There was no relationship between θ and any of the environmental or management variables with a stable mean value of 0.96. For α, individual functions were required for temperature (10–31 °C), nitrogen (N) concentration (1.5–5.9% N), water status (expressed as pre-dawn leaf water potential, ψ_lp) (−0.01 to −1.6 MPa), regrowth duration (20–60 days), and different times (up to 180 min) under moderate (850–950 μmol m⁻² s⁻¹ photon irradiance) and severe (85–95 μmol m⁻² s⁻¹ photon irradiance) shade. The highest α of 0.036 μmol CO₂/μmol photon irradiance was found in non-limiting conditions and defined as the standardised maximum (α_s=1). Values of α_s=1 were measured in optimum ranges of 10–24 °C, 4.0–5.9% N, −0.01 to −1.0 MPa and 20 days regrowth. In addition, values of α_s reached a steady-state asymptote of 0.74 after 60 min of severe shade and 0.92 after 40 min of moderate shade. Individual functions of α could not be integrated into a simple multiplicative model but a ‘law of the minimum factor’ model was appropriate. Predicted results from this model were then validated with 46 independent data points collected when at least two factors were outside their optimum range. The model accounted for 88% of the variation in observed α values. This research has derived functional relationships for α that can be used to assist predictions of leaf photosynthesis and ultimately pasture growth by their inclusion in canopy photosynthesis models.