New Ichneumonidae (Hymenoptera) parasitoids of forest insect pests in Bulgaria

During the period 1989–1998 investigations were carried out on the parasitoids of some forest insect pests in Bulgaria. Twenty-one ichneumonid species are reported for the first time in Bulgaria as parasitoids of different coleopteran, lepidopteran, and hymenopteran hosts. From these, 6 parasitoids are new records for the parasitoid complexes of the hosts:Exochus decoratus Holmgr. onEudemis profundana (Den. & Schiff.) andGelechia turpella (Den. & Schiff.);Lissonota culiciformis Grav.—onParanthrene tabaniformis (Rott.);Lissonota unicincta Holmgr.—onG. turpella; Xorides gracilicornis (Grav.)—onXylotrechus sp.; andEriborus terebrator Aubert—onClostera anastomosis (L.). Most parasitoids developed as primary parasitoids in the hosts.Itoplectis alternans (Grav.) is a primary parasitoid ofNycteola asiatica (Krul.) and a hyperparasitoid ofE. terebrator: Acropimpla pictipes (Grav.) is secondary parasitoid onAnacampsis populella (Cl.). With the exception of two species ofOlesicampes genus, which destroyed over 50% ofStauronematus compressicornis (F.) andPristophora conjugata (Dahlm.) larvae, andE. terebrator which killed 15.4% ofC. anastomosis population, the rest of the parasitoids occurred in low densities, and did not play an important role in reducing the number of their hosts.     

佳木斯园林绿地寄生性天敌昆虫调查及生物防治

在大学生科技创新项目的资助下,对佳木斯公园、绿化区的园林绿地进行的寄生性天敌昆虫资源调查,共采集得到寄生性昆虫共7科29种427只,这些寄生性天敌昆虫已经对园林绿地害虫有较好的防控作用。论述了寄生性天敌昆虫在农林业生产中做为生物防治载体的应用前景。     

Beyond host specificity: the biotechnological exploitation of chitolectin from teratocytes of Toxoneuron nigriceps to control non-permissive hosts

Journal of Pest Science - The virulence factors (VF) that parasitoids use to regulate the growth and development of their hosts have potential as new events in transgenic plants. VFs are promising...     

叶蜂类天敌姬蜂研究进展

记述了我国主要森林害虫叶蜂类天敌姬蜂种类及其寄生特点、控制作用、研究现状，并对姬蜂的应用前景和研究方向进行了讨论。     

Primary studies on the parasitoid fauna of Coccoidea in cultivated and non-cultivated areas in the east Mediterranean region of Turkey

The parasitoid fauna of Coccoidea in cultivated and noncultivated areas was studied in the east Mediterranean region of Turkey in 1994–1997. In total, 25 parasitoid species belonging to Aphelinidae, Encyrtidae, and Eulophidae (Hymenoptera) were reared from 21 different scale hosts. On crop plants, mainly citrus and peach, 13 parasitoids from eleven Coccoidea hosts were determined while 14 parasitoid species were obtained from twelve scale insects on non-cultivated plants. Seven parasitoid species are new records for the Turkish insect fauna. Only one of them was found on citrus, while the other six species were sampled in non-cultivated areas. The most common parasitoids encountered wereAphytis melinus DeBach,Encarsia berlesei (Howard) (Aphelinidae),comperiella bifasciata Howard (Encyrtidae), andMetaphycus flavus (Howard) (Eulophidae) in cultivated areas andCoccophagus lycimnia (Walker) (Aphelinidae) in non-cultivated areas. All other parasitoids species were obtained only from a few scale insects hosts.     

The effect of oviposition experience of adult Microplitis pallidipes parasitoids on discrimination between nucleopolyhedrovirus-infected and healthy Spodoptera litura larvae

Using multiple pest-mortality sources is a basic tenet of IPM, but they may not always be compatible. To that end, we examined compatibility of parasitism and nucleopolyhedrovirus (NPV) infected. Specifically, we examined the effects of prior oviposition experience by Microplitis pallidipes on its ability to distinguish NPV-infected from healthy larvae of Spodoptera litura. We found that time spent searching for hosts was significantly lower, while the number of attacks, percentage of first attacks, and parasitism rate were all significantly higher in healthy versus virus-infected hosts for parasitoids that had experienced healthy hosts. Meanwhile, parasitoids that had experienced infected hosts spent significantly more time searching for infected hosts starting on day 2. For these parasitoids, the number of attacks, percentage of first attacks, and parasitism rate were all significantly lower from day 2 in virus-infected versus healthy larvae. We found no significant differences between parasitoids without experience versus ones experienced with infected hosts in search time for infected hosts, number of infected host attacks, or percentage of first attacks on infected hosts. In comparison, parasitoids that had experienced healthy hosts spent less time searching for healthy hosts than parasitoids without experience, and displayed a higher frequency of attack on healthy hosts and a higher percentage of first attacks on healthy hosts. Experience with healthy larvae aided parasitoids in distinguishing healthy from virus-infected larvae, but experience with virus-infected larvae did not convey the same discrimination. This information provides a better understanding of how to integrate NPV applications with naturally occurring parasitism for this pest in crops.     

Weeds,aphids, and specialist parasitoids and predators benefit differently from organic and conventional cropping of winter cereals

The aphid–natural enemy interaction in winter wheat fields constitutes a complex system that has been frequently studied because of its implication for biological control. However, not all of the aphids living in cereal fields are crop pests, as there are also aphids living on weeds that may serve as alternative hosts or prey for aphid parasitoids or predators. In this context, a concomitant survey of the plant and insect communities was conducted to understand how different plant communities affect the abundance and richness of aphids and the interactions with their natural enemies. The plant community was split into functional groups (grasses, legumes and forbs), and the aphid community was divided into feeding groups according to their host preferences (specialists in grasses or forbs). The grass aphids, which dominated the total aphid catches, responded positively to grass cover, which was particularly enhanced in the conventional fields. Conversely, the forb aphids, which mainly conditioned the total species richness of the aphids, were closely correlated with the local abundance of legumes. The system of cereal aphid-parasitoids was enhanced in the conventional fields, where the abundance of grasses was higher, whereas the legumes of the organic fields indirectly played a key role in enhancing the richness of the parasitoids and the abundance of predators. Our findings indicate that a bottom-up effect exists throughout the plant community, aphids, and aphidophagous insects and that plant community characteristics should be considered to better understand cereal aphid control.     

Effects of single-tree selection harvesting on hymenopteran and saproxylic insect assemblages in the canopy and understory of northern temperate forests

Insects respond to changes in microhabitat caused by canopy disturbance, and thus can be used to examine the ecological impacts of harvesting. Single-tree selection harvesting is the most common silvicultural system used to emulate local small-scale natural disturbance and maintain uneven-aged forest structure in temperate forests. Here, we test for differences in richness, abundance, and composition of hymenopteran and saproxylic insect assemblages at four different taxon levels (selected insect orders; and all hymenopteran families, and braconid subfamilies and morphospecies) between the canopy and understory of unharvested and single-tree selection harvested sites in a northern temperate forest from central Canada. Harvesting had no effect on insect assemblage richness, composition or abundance at the three highest taxon levels (order, family and subfamily). Similarly, richness and abundance at the lowest-taxon level (braconid morphospecies) were similar, although composition differed slightly between unharvested and harvested stands. Insect assemblages were vertically stratified, with generally higher abundance (for Diptera, Hymenoptera, some hymenopteran families and braconid subfamilies) and richness (for braconid morphospecies) in the understory than the canopy. In particular, composition of the braconid morphospecies assemblage showed relatively low similarity between the understory and canopy. Single-tree selection harvesting appears to influ-ence wood-associated insect taxa only subtly through small changes in community composition at the lowest taxon level, and thus is recommended as a conservative approach for managing these northern temperate forests.     

红蜡蚧寄生蜂种类订正研究(膜翅目:小蜂总科)

我国红蜡蚧的寄生蜂共记载有30种，文章根据各种蜂的生物学习性，对这些寄生蜂进行了订正研究，确定其中19种为红蜡蚧的初寄生蜂，其中跳小蜂科Encyrtidae 10种：红蜡蚧扁角跳小蜂Anicetus beneficus Ishii et Yasumatsu、霍氏扁角跳小蜂A.howardi Hayat,Alam et Agarwal、红帽蜡蚧扁角跳小蜂A.ohgushii Tachikawa、食红扁角跳小蜂A.rubensi Xu et He、寡毛扁角跳小蜂A.rarisetus Xu et He、柯氏花翅跳小蜂Microterys clauseni Compere、聂特花翅跳小蜂M.nietneri (Motschulsky）、红黄花翅跳小蜂M.rufofulvus Ishii、美丽花翅跳小蜂M.speciosus Ishii、匀色花翅跳小蜂M.unicoloris Xu；蚜小蜂科Aphelinidae 7种：斑翅食蚧蚜小蜂Coccophagus ceroplastae (Howard）、夏威夷食蚧蚜小蜂C.hawaiiensis Timberlake、赛黄盾食蚧蚜小蜂C.ishiii Compere、日本食蚧蚜小蜂C.japonicus Compere、赖食蚧蚜小蜂C.lycimnia (Walker）、黑色食蚧蚜小蜂C.yashidae Nakayama、蜡蚧斑翅蚜小蜂Aneristus ceroplastae Howard;金小蜂科Pteromalidae 1种：盔蚧短腹金小蜂Anysis saissetiae (Ashmead）；姬小蜂科Eulophidae 1种：蜡蚧啮小蜂Tetrastichus ceroplastae (Girault)。5种为红蜡蚧的重寄生蜂：粉蚧克氏跳小蜂Clausenia purpurea Ishii、敛眼优赛跳小蜂Eusemion comigerum (Walker）、褐软蚧尖角跳小蜂Pareusemion studiosum Ishii、微食皂马跳小蜂Zaomma lambinus(Walker)、日本方梗跳小蜂Cerapteroceroides japonicus Ashmead,其他6种为误订。另外，还记述了各种寄生蜂的寄主及分布，以便参考。     

红树林自然保护区无瓣海桑主要害虫及其寄生性天敌种类调查研究

2009～2011年,采用平行取样法,在广西钦州康熙岭红树林自然保护区无瓣海桑纯林内,对无瓣海桑主要害虫及其寄生性天敌进行调查,分叶部、枝梢及蛀干害虫,按轻、中、重3级划分危害程度,结果表明,在研究地调查收集到无瓣海桑害虫种类共4目13科21种,其中绿黄枯叶蛾、木麻黄枯叶蛾、迹斑绿刺蛾、白囊袋蛾和荔枝茸毒蛾是无瓣海桑的主要害虫,均为食叶类害虫。并收集了危害无瓣海桑较严重的绿黄枯叶蛾及木麻黄枯叶蛾的寄生性天敌,共2目4科8种,其中绿黄枯叶蛾卵的寄生蜂,寄生率达67.3%。因此,保护和利用天敌是无瓣海桑害虫防治的有效方法。     

Mass rearing of <Emphasis Type="Italic">Neochrysocharis formosa</Emphasis> (Westwood) (Eulophidae: Hymenoptera), a parasitoid of leafminers (Agromyzidae: Diptera)

A method for mass rearing the parasitoid Neochrysocharis formosa (Westwood) was developed, using the leafminer Liriomyza huidobrensis (Blanchard) as insect host, and the Phaseolus vulgaris L. bean as host plant. About 1,500 parasitoids per tray of 84 plants emerged from about 2,000 leafminer larvae; 70% of these were females. The most suitable release density of host was two pairs of leafminer adults per bean plant, and that of parasitoid was three pairs per 24 leafminer larvae. Adult parasitoids were harvested based on positive photo-tactic response with 94.5% efficiency. Plants were improved by trimming their tips to increase the leaf area. The trimming process increased the number of leafminer larvae by about 32%. Furthermore, nitrogen fertilization of plants overcame the size reduction of the insects due to continuous rearing. Rearing of leafminer under light—dark (16:8 h) cycle decreased the development period of larvae and pupae. In addition, it restricted the time and duration of larval emergence and adult eclosion. Storage of parasitoid males and females at 7 and 10°C ± 0.5, respectively, proved to be optimal. The fertility of the mass-produced parasitoids after 1 year of rearing reduced by about 23%, and the sex ratio shifted toward males. In conclusion, successful mass production of N. formosa was achieved through designing suitable rearing cage and an efficient harvesting device, improving the host plant, and determining the age and the conditions for cold storage of the parasitoid and its host. Next, we checked the characteristics of the mass-produced wasps to ensure the efficacy when used in the field.     

Model selection and averaging in the estimation of population parameters of <Emphasis Type="Italic">Bemisia tabaci</Emphasis> (Gennadius) from stage frequency data in sweet pepper plants

Bemisia tabaci is a significant pest for many crops, but there are few population studies of this insect on sweet pepper (Capsicum annuum). In this study, stage frequency data were generated with B. tabaci in sweet pepper plants in various situations, and the Bellows and Birley method was used to obtain population parameters from the data. The Akaike Information Criterion (AIC) was used to select the best option of the Bellows and Birley method and, in some cases, to estimate the parameters of the population using model averaging. The ratios estimated/observed for each population parameter were calculated to assess bias and were used to correct the estimations if the ratios were different from 1. The effects of different factors on the estimations of population parameters were analysed. The total duration of development was affected by the experimental conditions (laboratory vs. greenhouse) and temperature, but it had the highest precision. The final survival rate was affected by temperature, and the estimation of individuals entering each stage was affected only by the options included in the Bellows and Birley method. AIC helped to detect differences in the daily survival rate among the different experiments between N1 (first instar) (range 0.842–0.923), and the egg (range 0.989–1.0) and N4 (fourth instar) (0.990). The methodology used can be employed in field population studies. For example, the final survival rate in the greenhouse experiments varied between 0.624 and 0.097, depending on if the parasitoids were present or not, and the total development varied between 420.6 and 440.7 degree days.     

Host acceptance behavior of the parasitoid Aphelinus mali and its aphid-host Eriosoma lanigerum on two Rosaceae plant species

The presence of a natural enemy in a habitat refuge is no guarantee of emigration by these into crop fields, when pest population outbreaks occur. Parasitoids from a refuge may not prefer foraging on the pest crop, exhibiting host fidelity, and therefore not constituting a source of natural enemies for improving biological control. An effective refuge must not only be a suitable sink for natural enemies, providing an acceptable host when these are not present in the crop, but it must also be a suitable source of parasitoids that readily accept the aphid-host on the crop. Therefore, crop-originated parasitoids would have to accept pests from the refuge as hosts to lay eggs in, and refuge-originated parasitoids would have to accept and lay eggs in pests from the crop. We here study the host fidelity of populations of Eriosoma lanigerum originating from two host plants (firethorn and apple) through reciprocal transfer experiments. Thereafter, the host fidelity of parasitoids from populations in the two host plants (firethorn and apple) was assessed. Reciprocal transfer experiments of parasitoids did not show an association between apple-originated parasitoids and their preference for any of the aphid hosts. Conversely, parasitoids from firethorn exhibited a higher number of attacks and in less time when aphids from apple were offered, suggesting a preference for apple-originated aphids. If future field work confirms these findings, firethorn could become an important management tool for enhancing biological control of woolly apple aphid in apple orchards, without being a substantial source of aphids.     

Temperature-dependent differences in biological traits between two strains of <Emphasis Type="Italic">Trichogramma cacoeciae</Emphasis> (Hymenoptera: Trichogrammatidae)

Parasitoids’ efficiency in controlling pests depends not only on their ability to parasitize their hosts but also on how much they are adapted to climatic conditions (notably temperature) of the area where they are planned to be released. In addition, the optimal conditions for production of parasitoids used for inundative releases like Trichogramma spp. may also vary largely as a function of strains. Using the parasitoid Trichogramma cacoeciae Marchal as biological model, we studied how temperature affects important parasitoid efficiency-related biological traits under laboratory conditions. Emergence, mortality rates and fecundity of two strains of T. cacoeciae, one originating from France (Alsace) and the other one from Tunisia (Degache), were compared at constant temperatures of 15, 20, 25 and 30°C. The parasitoids of the French strain showed highest fecundity at 25°C with wasps that had been reared at 20 or 25°C. The Tunisian strain showed the highest fecundity at 25°C, but only when wasps were previously reared at 25 or 30°C. For both strains, the highest mortality occurred among wasps that had laid eggs at 30°C. Emergence rates were relatively high at all temperatures, although the French strain did better at 15–25°C and the Tunisian one at 20–30°C. Because of the differences in biological traits of these two T. cacoeciae strains in relation to the temperature, a judicious choice must be made among the various strains when using this species in biological control programs.     

马尾松毛虫蛹、卵期寄生性天敌调查初报

通过对常山县松毛虫蛹、卵期寄生性天敌的调查，初步查得寄生蜂7科25种，寄生蝇2科7种，并分析子寄生性。     

Effects of water stress on emission of volatile organic compounds by <Emphasis Type="Italic">Vicia faba</Emphasis>, and consequences for attraction of the egg parasitoid <Emphasis Type="Italic">Trissolcus basalis</Emphasis>

When plants are damaged by herbivorous insects, blends of volatile organic compounds (VOCs) are induced and released and can also be used by parasitoids to locate hosts. The aim was to determine whether VOCs induced by water stress affect the plant–herbivore–parasitoid system represented by broad bean (Vicia faba; Fabales: Fabaceae) stink bug (Nezara viridula; Heteroptera: Pentatomidae) egg parasitoid (Trissolcus basalis; Hymenoptera: Platygastridae). The effects of water stress (expressed as the percentage fraction of transpirable soil water [FTSW] supplied) alone and in combination with N. viridula damage (feeding plus oviposition) were determined according to: (1) the behavioural response of the egg parasitoid in a Y-tube olfactometer and (2) the plant VOCs collected and analysed by thermal desorption–gas chromatography–mass spectrometry. With pot water capacity as FTSW₁₀₀, water stress was applied as mild (FTSW₈₀), moderate (FTSW₅₀) and severe (FTSW₁₀). Bioassays with plants under abiotic stress alone showed that egg parasitoids are more attracted by FTSW₁₀ plants than by well-watered plants. When plants were under abiotic and biotic stress interactions, the egg parasitoids are more attracted by FTSW₁₀ and FTSW₅₀ plants than by well-watered plants infested with N. viridula. Considering VOCs emissions, projection to latent structures discriminant analysis (PLS-DA) separated treatments according to egg parasitoid responses. Water stress alone and in combination with biotic stress induced changes in VOC emissions of V. faba plants that attract egg parasitoids. These findings contribute to our understanding of how water stress affects the interactions between plants, insect pests and egg parasitoids.     

Parasitoid wasps on <Emphasis Type="Italic">Epinotia granitalis</Emphasis> (Lepidoptera: Tortricidae) larvae

 Parasitism by parasitoid wasps on the larvae of Epinotia granitalis (Butler), which is a wood-injuring pest in coniferous plantations, was observed for 6 years in two plantation forests of Chamaecyparis obtusa (Sieb. et Zucc.) Endl. and Cryptomeria japonica (L.f.) D. Don that were adjacent to each other and consisted of trees of almost the same size. E. granitalis larvae were collected from early April to mid-May, then reared in glass bottles, and finally examined to determine whether or not the larvae had been parasitized. Five species of parasitoids were recorded; two of them were classified in Ichneumonidae, Campoplex spp. A and B, and others in Braconidae, Iconella repleta Papp, Bassus cingulipes Nees von Esenbeck, and Bracon spp. In all 6 years, parasitism was first observed around 20 April. Species composition of parasitoids differed greatly among years and between forests. In 1 year in each forest the percentage parasitism increased significantly with the sampling date, indicating that not all parasitoids attack the same developmental stage of E. granitalis. The mean percentage parasitism per year was significantly higher in the forest of C. obtusa [11.5(%) ± 5.7(SD)] than in the forest of C. japonica (3.0 ± 4.1). These results suggest that the efficiency of parasitoid adults in finding hosts differed between the two forests. Received: September 20, 2001 / Accepted: August 5, 2002 Acknowledgments We are grateful to Dr. K. Maeto, Shikoku Research Center, Forestry and Forest Products Research Institute, and to Dr. K. Konishi, National Agricultural Research Center for Hokkaido Region, for identifying parasitoids, and to Dr. T. Miyaura, Forest Tree Breeding Center, and Dr. H. Kosaka, Forestry and Forest Products Research Institute, for their helpful advice throughout the study. Correspondence to:K. Kato     

Laboratory rearing of the solitary egg-larval parasitoid, Chelonus oculator Panzer (Hymenoptera: Braconidae) on a newly recorded factitious host Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae)

Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) is recorded as a new factitious host for the solitary egg-larval parasitoid, Chelonus oculator Panzer (Hymenoptera: Braconidae). A short biology and rearing method of the parasitoid on the new host were studied at 30±1°C, 60–70% relative humidity, with a photoperiod of 16:8 (L: D). Chelonus eclosed from 74.60% of parasitized hosts. No significant difference was found between development times of male and female parasitoids. Development of the parasitoid was completed in 28.14±0.47 days in male, and 28.87±0.58 days in female. However, a significant difference was found between adult sizes of male and female parasitoids. Adult dry mass was found 0.88±0.04 mg in male, and 1.99±0.11 mg in female. In addition, C. oculator was successfully reared from P. interpunctella in twenty generations with the explained method. Significant knowledge about biology of C. oculator is still lacking. However, the parasitoid can be candidate for future research as a biological control agent against some important lepidopteran pests, and P. interpunctella may be suitable factitious host for mass rearing of C. oculator.     

Canopy vs. understory: Does tree diversity affect bee and wasp communities and their natural enemies across forest strata?

Bees and wasps provide important ecosystem services such as pollination and biocontrol in crop-dominated landscapes, but surprisingly little information is available on hymenopteran communities in temperate forest ecosystems. Species richness and abundance of bees and wasps can be hypothesised to increase with plant diversity, structural complexity, and availability of food and nesting resources. By experimentally exposing standardised nesting sites, we examined abundance and species richness of cavity-nesting bees (pollinators), wasps (predators) and their associated parasitoids across a tree diversity gradient in a temperate deciduous forest habitat. In addition, spatial distribution of individuals and species across forest strata (canopy vs. understory) was tested. Abundance and species richness was high for predatory wasps, but generally low for pollinators. Species-rich forest stands supported increased abundance, but not species richness, of pollinators and predatory wasps, and also increased abundance and species richness of natural enemies. In addition, the forests showed a distinct spatial stratification in that abundance of bees, wasps and parasitoids as well as parasitism rates were higher in the canopy than understory. We conclude that particularly the canopy in temperate forest stands can serve as an important habitat for predatory wasp species and natural enemies, but not bee pollinators. Enhanced tree diversity was related to increased hymenopteran abundance, which is likely to be linked to an increase in nesting and food resources in mixed forest stands.     

伊藤厚丝叶蜂寄生天敌研究

报道寄生伊藤厚丝叶蜂Pachynematus itoi Okutani的寄生性天敌10种：毛瘤角蜂Pleolophus setiferae(Uahida,1936)（中国新记录），短翅田猎蜂Agrothereutes abbreviatus(Fabricius)，辽宁恩姬蜂Endasys liaoningensis Wang et Ma,大田猎姬蜂Agrothereutes macroincubitor(Uchida)，毛面泥甲姬蜂Bathythrix eilifacialis Sheng,狭颊刻姬蜂Arenetra Genangusta Sheng et Zhang，克禄格翠金小蜂Tritneptis klugii(Ratzeburg,1844)（中国新记录），暗尖胸青蜂Cleptes semiauratus(L.)，布朗撵寄绳Myxexoristops blondeli(Robineau-Desvoidy)，球孢白僵菌Beauveria bassiana，简要说明了它们的其它主要寄主及分布，对毛瘤角姬蜂和短翅田猎姬蜂进行了重新描述，指出了短翅田猎姬蜂的变异性，对寄生天敌的利用作了简要评价。