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1.
Hu Sun Jing-Ze Zhang 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(3):459-469
Colletotrichum isolates isolated from cowpea in the Hangzhou area of China were identified as C. destructivum based on morphological characteristics, pathogenicity tests, sequence analysis of the internal transcribed spacer (ITS)1,
5.8S RNA gene and ITS2 regions of ribosomal DNA and the infection process. The ability of the C. destructivum isolates to infect Arabidopsis thaliana was investigated under laboratory conditions and showed a two-phase hemibiotrophic infection process. In addition, the sequences
of the rDNA ITS region of C. destructivum isolates from cowpea were identical with 100% similarity to that of isolates of C. higginsianum originating from cruciferous plants. This article presents new evidence in support of C. higginsianum as a synonym of C. destructivum. 相似文献
2.
Severe spotting, blight and drop of leaves caused by Colletotrichum dematium were found on potted plants of Polygonatum falcatum, a liliaceous ornamental, in open fields in Kagawa Prefecture, Japan, in May 2001. This new disease was named anthracnose
of P. falcatum.
Keisuke Tomioka, Jouji Moriwaki, Toyozo Sato contributed equally to this work.
The fungal isolate and its nucleotide sequence data obtained in this study were deposited in Genebank, National Institute
of Agrobiological Sciences and the DDBJ/EMBL/GenBank databases under accessions MAFF239500 and AB334523, respectively. 相似文献
3.
Mikiko Harada Satoshi Ishikawa Tadaaki Hibi Kyoko Watanabe 《Journal of General Plant Pathology》2008,74(4):341-343
In 2003–2004, anthracnoses of Enkianthus campanulatus and Rhynchosia acuminatifolia were found for the first time in Kanagawa Prefecture and Tokyo in Japan. These pathogens were identified as Colletotrichum gloeosporioides based on their pathogenicity, morphology and ribosomal DNA spacer sequences.
Results were presented at the annual meeting of The Phytopathological Society of Japan in 2004. 相似文献
4.
Masaharu Kubota Kazufumi Nishi Emi Kato Fumihiro Terami 《Journal of General Plant Pathology》2011,77(1):68-71
In July 2006, black rot was observed on the leaves of 4-leaf-stage seedlings of salt-wort (Salsola komarovii) in Yamagata Prefecture, Japan. We isolated two single-conidial isolates from the diseased leaves. Although colony appearance
of the isolates was different from that of each other, both isolates were identified as Colletotrichum truncatum by morphology and molecular similarity. After inoculation of healthy salt-wort plants with the isolates, the isolates were
reisolated from symptomatic plants. We thus propose a new disease, anthracnose of salt-wort. 相似文献
5.
Masamichi Isogai Koji Ishii Seisaku Umemoto Manabu Watanabe Nobuyuki Yoshikawa 《Journal of General Plant Pathology》2009,75(2):140-143
Virus-like symptoms—red ringspots on stems and leaves, circular blotches or pale spots on fruit—were found on commercial highbush
blueberry (Vaccinium
corymbosum) cultivars Blueray, Weymouth, Duke and Sierra in Japan. In PCR testing, single DNA fragments were amplified from total nucleic
acid samples of the diseased blueberry bushes using primers specific to Blueberry red ringspot virus (BRRV). Sequencing analysis of the amplified products revealed 95.7–97.7% nucleotide sequence identity with the BRRV genome.
This paper is the first report of blueberry red ringspot disease caused by BRRV in Japan.
The nucleotide sequence data reported in this paper are available in the GenBank/EMBL/DDBJ database as accessions AB469884
to AB469893 for BRRV isolates from Japan. 相似文献
6.
Edit Ludmerszki Sengnirane Chounramany Csilla Oláh György Kátay Ilona Rácz Asztéria Almási Ádám Solti Iván Bélai Szabolcs Rudnóy 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(1):145-156
This study aimed to detect the harmful effects of Maize dwarf mosaic virus (MDMV) infection, and to demonstrate the potential benefits of S-methylmethionine-salicylate (MMS) pretreatment in infected maize (Zea mays L.) plants. The results of chlorophyll a fluorescence measurements showed that in MDMV-infected plants additional quenchers of fluorescence appear, probably as the result of associations between the virus coat protein and thylakoid membranes. It is important to note that when infected plants were pretreated with MMS, such associations were not formed. MDMV infection and MMS pretreatment resulted in a decrease in ascorbate peroxidase (APX) activity in maize leaves, while infection contributed to an increase in activity in the roots. Infection raised the guaiacol peroxidase (GPX) enzyme activity level, which was reduced by MMS pretreatment. MMS contributed to a decrease in both the RNA and coat protein content of MDMV, to an equal extent in maize leaves and roots. The results showed that MMS pretreatment enhanced the stress response reactions against MDMV infection in maize plants and retarded the spreading of infection. 相似文献
7.
Rudra P. Singh Andrea D. Dilworth Xiaoping Ao Mathuresh Singh Virendra K. Baranwal 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(4):691-694
Surveys of Impatiens and Verbena species in local nurseries in Fredericton, Canada and Verbena species in New Delhi, India showed widespread infection of Citrus exocortis viroid (CEVd) in vegetatively-propagated and seed-grown plants. To determine viroid seed transmission, samples of eight varieties
of Impatiens and 11 varieties of Verbena were obtained from four commercial sources. All 19 samples collected contained viroid infection irrespective of variety.
The presence of viroid in non-germinated seed was 21%, while the transmission rate in seedlings was 66% in Impatiens walleriana in 2006. Following 2 years of seed storage, the respective figures were 6% and 26%. Similarly, in Verbena x hybrida the presence of viroid in seed was 13% in 2006 with a seed-transmission rate in seedlings of 28%, while the respective figures
after 2 years of storage were 5% and 45%. 相似文献
8.
Hiroshi Kajihara Kazuyuki Muramoto Shin-ichi Fuji Shuhei Tanaka Shin-ichi Ito 《Journal of General Plant Pathology》2009,75(1):72-75
To detect Japanese yam mosaic virus (JYMV) and Yam mild mosaic virus (YMMV) in yam plants in Japan, we developed a duplex RT-PCR assay consisting of a tube-capture procedure followed by one-step
RT-PCR with two primer pairs. A 241-bp fragment of the coat protein region of JYMV and a 174-bp fragment of the nuclear inclusion
protein b region of YMMV were amplified, thus identifying the two viruses from yam plants cultivated in Yamaguchi Prefecture
in 2007. All water yam plants examined were infected with YMMV alone. All the Japanese yam and Chinese yam plants were infected
with either JYMV alone or both JYMV and YMMV, suggesting that YMMV and JYMV are prevalent among field-grown yam plants. 相似文献
9.
Random insertional mutagenesis using a marker DNA fragment is an effective method for identifying fungal genes relevant to morphogenesis, metabolism, and so on. Agrobacterium tumefaciens-mediated transformation (AtMT) has long been used as a tool for the genetic modification of a wide range of plant species. Recent study has indicated that A. tumefaciens could transfer T-DNA not only to plant cells but also to fungal cells. In this study, AtMT was applied to Colletotrichum lagenarium for random insertional mutagenesis. We constructed a binary vector pBIG2RHPH2 carrying a hygromycin-resistant gene cassette between the right and left borders of T-DNA. Optimal co-cultivation of C. lagenarium wild-type 104-T with pBIG2RHPH2-introduced A. tumefaciens C58C1 led to the production of 150–300 hygromycin-resistant transformants per 106 conidia. Southern blot analysis revealed that T-DNA was mainly integrated at a single site in the genome and at different sites in transformants. The T-DNA inserts showed small truncations of either end, but the hygromycin-resistant gene cassette inside the T-DNA was generally intact. The mode of T-DNA insertion described above resulted in highly efficient gene recovery from the transformants by thermal asymmetrical interlaced-polymerase chain reaction. The fungal genomic DNA segments flanking T-DNA were identified from five of eight mutants that had defective melanin biosynthesis. The sequence from one of the segments was identical to that of the melanin biosynthesis gene PKS1 of C. lagenarium, which we previously characterized. These results strongly support our notion that AtMT is a possible tool for tagging genes relevant to pathogenicity in the plant pathogenic fungus C. lagenarium. 相似文献
10.
11.
In October 2001, anthracnose caused by Colletotrichum acutatum Simmonds ex Simmonds was found on three-leaf akebia (Akebia trifoliata) in Saitama, Japan. This is the first report of anthracnose on three-leaf akebia caused by C. acutatum. 相似文献
12.
Keisuke Tomioka Junji Nishikawa Jouji Moriwaki Toyozo Sato 《Journal of General Plant Pathology》2011,77(1):60-63
Severe spotting and blight of leaves caused by Colletotrichum destructivum were found on snapdragon (Antirrhinum majus L.), a scrophulariaceous ornamental, in open fields in Shizuoka Prefecture, Japan, from June through September 2004. The
fungus is added to the group of the pathogens causing anthracnose of snapdragon. 相似文献
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15.
Khalid A. Hussein Mohamed A. A. Abdel-Rahman Ahmed Y. Abdel-Mallek Saad S. El-Maraghy Jin Ho Joo 《Phytoparasitica》2012,40(2):117-126
The greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) is occasionally found in beehives and is a major pest of stored wax. Entomopathogenic fungi have
recently received attention as possible biocontrol elements for certain insect pests. In this study, 90 isolates of Beauveria bassiana and 15 isolates of Metarhizium anisopliae were screened for proteases and lipases production. The results showed significant variations in the enzymatic action between
the isolates. In the bioassay, the selected isolates evinced high virulence against the 4th instar of the G. mellonella larvae. The isolates BbaAUMC3076, BbaAUMC3263 and ManAUMC3085 realized 100% mortality at concentrations of 5.5 × 106 conidia ml−1, 5.86 × 105 conidia ml−1, and 4.8 × 106 conidia ml−1, respectively. Strong enzymatic activities in vitro did not necessarily indicate high virulence against the tested insect pest. The cuticle of the infected larvae became dark
and black-spotted, indicating direct attack of fungus on the defense system of the insects. The LC50 values were 1.43 × 103, 1.04 × 105 and 5.06 × 104 for Bba3263AUMC, Bba3076AUMC and Man3085AUMC, respectively, and their slopes were determined by computerized probit analysis
program as 0.738 ± 0.008, 0.635 ± 0.007 and 1.120 ± 0.024, respectively. 相似文献
16.
From the genome of a Japanese field isolate of the rice blast fungus, Magnaporthe oryzae, we newly identified Inago1 and Inago2 LTR retrotransposons. Both elements were found to be Ty3/gypsy-like elements whose copies were dispersed within the genome of Magnaporthe spp. isolates infecting rice and other monocot plants. Southern hybridization patterns of nine re-isolates derived from conidia
of the strain Ina168 produced after a methyl viologen treatment were not changed, indicating that the insertion pattern of
Inago elements is relatively stable. 相似文献
17.
Robert Czajkowski Grzegorz J. Grabe Jan M. van der Wolf 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(2):263-275
Detailed studies were conducted on the distribution of Pectobacterium carotovorum subsp. carotovorum and Dickeya spp. in two potato seed lots of different cultivars harvested from blackleg-diseased crops. Composite samples of six different
tuber sections (peel, stolon end, and peeled potato tissue 0.5, 1.0, 2.0 and 4.0 cm from the stolon end) were analysed by
enrichment PCR, and CVP plating followed by colony PCR on the resulting cavity-forming bacteria. Seed lots were contaminated
with Dickeya spp. and P. carotovorum subsp. carotovorum (Pcc), but not with P. atrosepticum. Dickeya spp. and Pcc were found at high concentrations in the stolon ends, whereas relatively low densities were found in the peel and
in deeper located potato tissue. Rep-PCR, 16S rDNA sequence analysis and biochemical assays, grouped all the Dickeya spp. isolates from the two potato seed lots as biovar 3. The implications of the results for the control of Pectobacterium and Dickeya spp., and sampling strategies in relation to seed testing, are discussed. 相似文献
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19.
Shigemitsu Kimura Susumu Tokumaru Kazuhiko Kuge 《Journal of General Plant Pathology》2009,75(4):322-324
Yeast-like fungi were isolated from lesions on azuki bean (cv. Shin-Kyotodainagon) seeds that had been sucked by bean bugs
in Kyoto Prefecture, Japan. On the basis of morphological and physiological characteristics and sequence data of the internal
transcribed spacer (ITS) regions including the 5.8S rDNA, these yeasts were identified as Eremothecium coryli and E. ashbyi. Pathogenicity of those yeasts was confirmed by a reinoculation test. To our knowledge, this is the first report of the occurrence
of yeast spot in azuki bean in Japan.
The nucleotide sequence data reported are available in the GeneBank/EMBL/DDBJ database as accessions AB478291–AB478309 for
E. coryli AZC1–19 and AB478310–AB478317 for E. ashbyi AZA1–8. 相似文献
20.
Akira Kawaguchi 《Journal of General Plant Pathology》2011,77(5):299-303
Forty-one strains of Rhizobium vitis, either tumorigenic (Ti) or nonpathogenic, were characterized using multilocus sequence analysis (MLSA) of the partial nucleotide
sequences of pyrG, recA, and rpoD. The strains separated into seven clades. Rhizobium vitis (Ti) strains isolated from Japan were divided into five genetic groups (A to E), and nonpathogenic R. vitis strains were divided into two genetic groups (F and G). This result suggests that there are new genetic groups of R. vitis in Japan. Among these groups, members of A and B groups are widely distributed throughout Japan. 相似文献