喀斯特生态系统土壤表面电化学特征及其影响因子

以西南喀斯特典型石漠化生态系统土壤为研究对象,采用空间替代时间的方法,研究石漠化演替过程中土壤表面电化学特征演变规律及其与土壤理化性质的相关性。采用物质表面联合分析法对不同石漠化等级土壤表面电化学属性及进行测定。结果表明,土壤表面电荷密度、表面电场强度、比表面、表面电荷数量随石漠化强度的增加而下降,其变化范围分别为0.34 C·m-2~0.42 C·m-2、4.85×108 V·m-1~5.86×108 V·m-1、47.11 m2·g-1~53.16 m2·g-1、16.86 cmol·kg-1~22.82 cmol·kg-1,土壤表面电位随石漠化强度的增加而上升,其变化范围为-113.74 mV~ -115.10 mV;研究区黏土矿物组成为伊利石、高岭石、绿泥石、伊蒙混层、绿蒙混层,且以绿蒙混层为主;土壤黏粒、砂粒、非晶质氧化铝、胡敏酸是影响喀斯特石漠化地区土壤表面电化学属性变化的主要因素,解释率分别为48.3%、38.1%、13.0%、12.0%;土壤粒径组成、有机质组分和金属氧化物对土壤表面电化学特征影响由强到弱依次分别为：土壤黏粒>砂粒>粉粒,胡敏酸>有机碳>富里酸,非晶质氧化铝>游离氧化铁>非晶质氧化铁。本研究对中国西南喀斯特石漠化土壤管理与调控、退化植被恢复重建具有重要意义。     

土壤碳转化对大气CO_2升高响应机制研究进展

土壤有机碳是地球表层最大的碳库,其微小的变化将影响大气CO_2浓度,而使土壤成为CO_2的源或汇。在大气CO_2浓度升高的大背景下,土壤碳库的转化与更新过程在时间和空间上发生改变。由于土壤本身及环境条件的差异性,土壤碳转化过程对CO_2浓度上升的响应差异较大,要阐明这些差异,理清土壤"碳汇(源)"功能,必须全面深入了解土壤碳转化对CO_2的响应机理。然而,长期以来,关于CO_2升高对土壤地上部碳输入的影响研究较多,而针对土壤有机碳对CO_2升高的响应,尤其是响应的内在机制研究不足。本文主要依据近10年相关研究报道,在论述CO_2浓度升高对土壤有机碳含量影响的基础上,从微生物学、土壤酶活性及与其他影响因素的耦合效应揭示CO_2浓度升高对土壤有机碳转化内在机制的研究进展,并展望了未来相关研究的主要方向,旨在为将来农田土壤管理决策提供科学的理论依据。     

杉木人工林土壤系统有机碳相关变量的通径分析

为了评价土壤性质对土壤有机碳含量的影响,运用通径分析方法研究了江西大岗山杉木人工林下土壤有机碳含量与土壤性质的关系。结果表明:在杉木人工林的整个发育过程中,土壤有机碳含量和土壤各性质表现出明显的规律性变化;土壤性质通过直接作用和间接作用共同影响着土壤有机碳的含量和分解转化方向,但不同土壤性质的影响机理不同。在土壤系统中,碱解氮量、细菌数量和真菌数量以及它们的共同作用决定了土壤有机碳含量的大部分变异,是影响土壤有机碳含量变化的主导因素,有效磷含量和放线菌数量由于直接正效应和间接负效应相抵,因而对土壤有机碳含量的决定程度较小,总孔隙度和速效钾含量对有机碳含量虽也表现出一定的直接效应影响,但影响程度较低;和简单相关或多元回归分析方法相比,通径分析能更加客观和全面地评价土壤性质对土壤有机碳含量的影响作用。     

辽东山区天然次生栎林土壤有机碳含量及其与理化性质的关系

通过对设置在辽东山区本溪市草河口镇和庄河市仙人洞镇两个典型天然次生栎林(Quercus spp.)样地内的土样进行分层(0—10,10—20,20—30,30—40cm)采集和室内分析,研究了0—40cm土层土壤有机碳、全氮、全磷等含量的剖面分布特征,探讨了有机碳与理化性质间的关系。结果表明:土壤有机碳及全氮、全磷、全钾、碱解氮、速效磷、速效钾、阳离子交换量的含量均随土层加深而降低,而土壤容重随土层加深而增加,由t检验和方差分析得知,各指标含量在土层间差异均达到5%显著水平;土壤有机碳与粉粒、pH、全氮、全磷、全钾、碱解氮、速效磷、速效钾、阳离子交换量间均呈显著正相关(r=0.430 9~0.919 0,n=44,P0.01),与土壤容重、砂粒间均呈显著负相关(r=0.388 3~0.670 6,n=44,P0.01),而与黏粒间无显著相关性;由逐步回归分析得知土壤碱解氮、碳氮比、全钾、pH、全氮与有机碳间关系最为紧密。土壤有机碳与其他理化性质间关系密切,且以全氮、碱解氮、碳氮比、全钾和pH对其影响最大;同时,各指标均具有明显的剖面分布规律。     

免耕对不同麦玉轮作农田土壤碳组分及理化性质的影响

为探讨耕作及轮作方式对农田土壤理化性质和碳组分的影响,设置免耕、传统耕作2种耕作方式,以及小麦-玉米轮作、小麦/玉米间作、小麦-冬油菜-玉米轮作3种种植模式,共形成6个处理,研究结果表明:与传统耕作相比,免耕增加了0~5 cm、5~20 cm土层全氮、全磷、速效磷和含水量,而降低了的土壤pH和土壤容重。免耕小麦/玉米间作(NT.W1/NT.WM.1)处理的土壤容重、含水量、全氮、全磷含量高于NT.WRM3和NT.WM5处理,在不同土层间,土壤全氮、全磷和速效磷含量随着土层深度的增加而降低。土壤碳组分含量总体表现为免耕处理高于传统耕作处理,免耕处理0~5 cm土层土壤有机碳、颗粒有机碳、可溶性有机碳、微生物量碳含量较相应传统耕作分别增加了1.31%~36.57%、2.07%~35.22%、2.38%~4.78%、2.08%~11.68%,在5~20 cm土层,免耕处理土壤有机碳和微生物量碳含量高于传统耕作。在不同免耕处理下,土壤有机碳,颗粒有机碳和微生物量碳含量在0~5 cm、5~20 cm土层总体表现为NT.WM5高于其他免耕处理,相关性分析表明,有机碳、微生物量碳和速效磷呈极显著正相关,容重和有机碳呈极显著负相关。综上所述,免耕小麦/玉米间作利于改善土壤理化性质,小麦-玉米轮作有利于提高土壤有机碳,颗粒有机碳和微生物量碳含量。     

岳桦和暗针叶林两种植被类型下土壤碳氮元素的转化过程

Soil samples were taken from an Ermans birch (Betula ermanii)-dark coniferous forest (Picea jezoensis and Abies nephrolepis) ecotone growing on volcanic ejecta in the northern slope of Changbai Mountains of Northeast China, to compare soil carbon (C) and nitrogen (N) transformations in the two forests. The soil type is Umbri-Gelic Cambosols in Chinese Soil Taxonomy. Soil samples were incubated aerobically at 20℃ and field capacity of 700 g kg^-1 over a period of 27 weeks. The amount of soil microbial biomass and net N mineralization were higher in the Ermans birch than the dark coniferous forest (P < 0.05), whereas the cumulative C mineralization (as CO₂ emission)in the dark coniferous forest exceeded that in the Ermans birch (P < 0.05). Release of the cumulative dissolved organic C and dissolved organic N were greater in the Ermans birch than the dark coniferous forest (P < 0.05). The results suggested that differences of forest types could result in considerable change in soil C and N transformations.     

高寒草原土壤有机碳与腐殖质碳变化及其微生物效应

基于多区域重复采样,研究了藏北高原不同状态(正常、轻度和严重退化)高寒草原表层(0～10 cm)、亚表层(10～20 cm)土壤有机碳(Soil organic carbon,SOC)、腐殖质碳(Humus carbon,HC)、胡敏酸碳(Humic acid carbon,HAC)和富里酸碳(Fulvic acid carbon,FAC)的变化,以及土壤微生物群落、微生物生物量碳(Microbial biomass carbon,MBC)、纤维素分解酶活性(Cellulolytic enzyme activity,CEA)对其产生的影响与作用。结果表明:高原寒旱环境中土壤的HC/SOC比例过低,但PQ值(HAC/HC)很高。随土层加深,不同状态草地SOC、HC、HAC含量、HC/SOC比例在总体上趋于不同程度的下降,PQ值则均呈一定程度的提高。相对于正常草地,随草地退化加剧,表层SOC、HC(HAC、FAC)增幅分别表现出略呈下降、大幅提高,亚表层降幅则均呈大幅下降。反映到0～20 cm土层,SOC、HC、HAC含量均表现出正常草地严重退化草地轻度退化草地,HC/SOC比例、PQ值则分别呈严重退化草地正常草地轻度退化草地、正常草地轻度退化草地严重退化草地,说明草地退化在促进表层SOC、HC(HAC、FAC)形成与积累的同时,更"激发"了亚表层的矿化,尤其是严重退化草地有机残体的分解过程,但腐殖质品质并未随土壤腐殖化程度的提高而得到相应改善。MBC、CEA与SOC、HC及组分高度一致的土体分布格局影响并决定了上述过程,草地退化有利于真菌、放线菌对土壤、尤其是亚表层土壤有机残体的分解与转化。     

电厂煤粉尘沉降特征及其对周边土壤主要性质的影响

通过室外定期观测及室内分析,研究了太原市某电厂储煤场周围下风向900 m范围的总降尘和煤粉尘的沉降规律,及其对该范围内土壤的主要化学性质和微生物数量的影响。结果表明,在此距离内,降尘总量和煤粉尘量随着离储煤场距离的增加而显著减少,离储煤场300 m的最近观测点在3月份降尘总量和煤粉尘量最高,分别为84.38和34.48 t.30 d-1.km-2。距污染中心越近的地方,土壤表层0～10 cm的有机碳及土壤活性有机质含量均显著增加,土壤微生物也随着煤粉尘降落量的增加有活跃的趋势。但是除土壤速效钾外,全氮及速效磷含量尚未有规律性变化。总之,煤粉尘的降落明显增加了土壤表层有机碳及土壤活性有机质的含量,同时也促进了土壤微生物的活跃。     

黑河上游冰沟流域不同林地土壤理化性质及有机碳和养分的剖面变化规律

在黑河上游的冰沟流域,采用野外采样和室内分析方法研究不同林地土壤理化性质及有机碳和养分的剖面变化规律.结果表明:不论是林地还是草地,土壤容重、物理性砂粒、pH值、CaCO3均随着土层的加深而增大;而孔隙度、团聚体、有机碳、全氮、全磷、CEC均随着土层的加深而降低.林地与草地土壤剖面的容重、物理性砂粒、pH值、CaCO3由小到大变化顺序为林地＜草地;而总孔隙度、团聚体CEC、有机碳、全氮、全磷由大到小变化顺序为林地＞草地.不同林地土壤剖面容重、物理性砂粒、pH值、CaCO3由小到大变化顺序为青海云杉＜高山灌丛林＜祁连圆柏林;而总孔隙度、团聚体CEC、有机碳、全氮、全磷由大到小变化顺序为青海云杉林＞高山灌丛林＞祁连圆柏林.     

开垦年限对干旱区土壤理化性质及剖面无机碳的影响

为阐明开垦年限对土壤理化性质及无机碳的影响,进一步了解干旱区土地开垦进程中的土壤质量变化规律和碳吸收与存储过程,以开垦年限分明的典型区—新疆三工河流域阜北农场为研究区,调查、分析了不同开垦年限(已开垦0,1,3,5,15,30,50a)剖面土壤的pH值、盐分、无机碳含量与储量的分布特征、变化规律及开垦年限对它们的影响。结果表明:(1)已开垦土地的土壤pH值随土层深度的增加而升高;随开垦年限的延长,0—60cm(耕作层)的土壤pH值显著降低(P0.05),60—140,140—200cm土壤pH值则升高。不同开垦年限的土壤盐分在剖面上呈S型分布,研究区土壤盐分为表聚型;随着开垦年限的延长,土壤盐分聚集层逐渐下移,各剖面平均含盐量减少。开垦历史的延长,有助于排盐排碱。(2)不同开垦年限的土壤无机碳含量与储量:0—60cm显著低于60—140cm,60—140cm显著低于140—200cm(P0.05)。随着土层深度的增加,土壤无机碳储量占全碳的比例增大。随着开垦年限的延长,0—60cm耕作层土壤无机碳含量、储量呈减少—增加—减少的变化趋势,无机碳储量占全碳的比例减少。与原始荒地相比,开垦50a时,0—60cm耕作层的土壤无机碳储量减少了55%;0—200cm土壤无机碳储量增加了11.74kg/m~2,无机碳储量所占全碳的比例降低了1.28%。(3)开垦年限的延长对土壤pH、盐分及无机碳的显著影响深度不同,分别集中于0—60cm耕作层,0—140cm及整个研究深度范围内。     

不同有机物料培肥对渭北旱塬土壤微生物学特性及土壤肥力的影响

通过田间试验,研究了施用不同有机物料对渭北旱塬耕地土壤微生物学特性及土壤肥力的影响。结果表明,化肥与不同有机物料配合施用,土壤微生物学特性［微生物量碳（MBC）、微生物量氮（MBN）、脲酶、碱性磷酸酶］以及部分土壤养分状况（全氮、速效磷、速效钾、阳离子交换量）比单施化肥处理均得到进一步改善。化肥配施秸秆堆肥处理效果最明显,其中微生物量碳增加了41.96%,微生物量氮增加了54.55%,脲酶活性增加了19.71%,碱性磷酸酶活性增加了7.35%,速效磷增加了63.12%;而且土壤微生物量碳、氮与速效磷、阳离子交换量呈显著正相关,微生物商（qMB）、脲酶活性、碱性磷酸酶活性与全氮、速效氮、速效钾含量呈显著相关;同时SMBC、SMBN、qMB等与作物产量密切相关（相关系数分别为0.85,0.74,0.82）。因此,化肥配施秸秆堆肥处理在渭北旱地雨热条件下对于全面提升土壤质量具有重要的意义;同时该区域土壤中微生物量碳氮与土壤养分状况、作物产量具有很好的一致性,可以表征土壤肥力状况及生产力水平。     

不同轮作制度下土壤中不稳定有机碳组分的变化

Taking Kenli County in the Yellow River Delta, China, as the study area and using digital satellite remote sensing techniques, cultivated land use changes and their corresponding driving forces were explored in this study. An interactive interpretation and a manual modification procedure were carried out to acquire cultivated land information. An overlay method based on classification results and a visual change detection method which was supported by land use maps were employed to detect the cultivated land changes. Based on the changes that were revealed and a spatial analysis between cultivated land use and related natural and socio-economic factors, the driving forces for cultivated land use changes in the study area were determined. The results showed a decrease in cultivated land in Kenli County of 5321.8 ha from 1987 to 1998, i.e., an average annual decrement of 483.8 ha, which occurred mainly in the central paddy field region and the northeast dry land region. Adverse human activities, soil salinization and water deficiencies were the driving forces that caused these cultivated land use changes.     

不同耕作方式对土壤有机碳、微生物量及酶活性的影响

【目的】依托8年长期(2005~2012)固定道定位试验,研究不同耕作方式对土壤有机碳、土壤微生物量、土壤酶活性在0—90 cm土层的分布特征,为优化中国西北干旱区的耕作方式提供理论依据。【方法】试验包括固定道垄作(PRB)、固定道平作(PFT)与传统耕作(CT)三种耕作模式下的土壤有机碳土壤总有机碳(TOC)、颗粒有机碳(POC)、土壤微生物量碳(MBC)、土壤微生物量氮(MBN)、土壤微生物量磷(MBP)、蔗糖酶、过氧化氢酶、脲酶及小麦产量进行了测定和分析。【结果】在0—90 cm土层,不同耕作方式下的TOC、POC、MBC、MBN、MBP、蔗糖酶活性、脲酶活性均随着土层的增加呈下降趋势,过氧化氢酶活性呈先下降后增大的分布特征;在0—60 cm,固定道保护性耕作能够显著增加心土层作物生长带土壤有机碳储量,有机碳储量大小为PRBPFTCT;PRB、PFT较CT可以显著增加0—10 cm作物生长带TOC、POC、MBC、MBN、MBP含量、蔗糖酶、脲酶活性,其大小为PRBPFTCT;耕作方式对过氧化氢酶活性影响不显著;TOC、POC、MBC、MBN、MBP、蔗糖酶活性、脲酶活性、过氧化氢酶活性之间均达到了显著或极显著相关。【结论】PRB较PFT、CT能够提高耕作层(0—10 cm)土壤有机碳含量、土壤微生物量、土壤酶活性, 增加作物产量, 增大0—60 cm土层有机碳储量,耕作方式(PRB、PFT及CT)对10 cm以下土层土壤环境改善作用不明显。     

Mineralization of dissolved organic carbon in mineral soil solution of two forest soils

Dissolved organic carbon (DOC) constitutes an important carbon input flux to forested mineral soils. Seepage from mineral subsoils contains only small amounts of DOC because of mineralization, sorption or the formation of particulate organic matter (POM). However, the relation between these processes is largely unknown. Therefore, the objective of this study was to quantify the mineralization of DOC from different depths of forest soils, and to determine degradation rate constants for rapidly and slowly degradable DOC pools. Mineralization of DOC and formation of POM in mineral soil solution from two forested sites in northern Bavaria (Germany) were quantified in a 97 days laboratory incubation experiment. Furthermore, spectroscopic properties such as specific UV absorption and a humification index derived from fluorescence emission spectrometry were measured before and after incubation. DOC in all samples turned out to belong mainly to the stable DOC pool (> 95 %) with half‐lives ranging from years to decades. Spectroscopic properties were not suitable to predict the mineralization of DOC from mineral soils. However, together with data on DOC from the forest floor and long‐term data on DOC concentrations in the field they helped to identify the processes involved in C sequestration in mineral subsoils. Mineralization, formation of POM, and probably sorption seem all to be responsible for maintaining low concentrations of DOC in the upper mineral soil. DOC below the upper mineral soil is highly resistant to mineralization, and thus the further decrease of DOC concentrations in the subsoil as observed under field conditions cannot be attributed to mineralization. Our results suggest that sorption and to some minor extent the formation of POM may be responsible for C sequestration in the subsoil.     

有机物料还田对华北小麦玉米两熟农田土壤有机碳及其组分的影响

中国农业废弃物种类多、数量大、利用率低、污染重。将有机物料还田,是实现废弃物资源化利用的重要途径。该研究以循环农业理念为指导,选择代表农田内循环的秸秆以及代表农沼循环、农牧循环、农菌循环、农工循环的废弃物沼渣、猪粪、菌渣和酒渣为试验材料,开展了等碳量还田定位试验,分析各有机物料还田后对土壤有机碳及其组分的影响。结果表明:1)连续施用有机物料提高了土壤总有机碳(TOC)、易氧化有机碳(LOC)、微生物量碳(MBC)和可溶性有机碳(DOC)含量。随着有机物料还田年限的增加,土壤TOC、LOC和MBC含量均不同程度地增加,年平均增幅分别为:15.57%~22.82%、20.00%~38.31%和16.30%~50.56%。还田5年后各有机物料处理土壤TOC、LOC、MBC和DOC含量平均分别是无机肥处理的1.24~1.62、2.07~3.19、1.20~2.06和1.05~3.36倍。2)不同有机物料中均利于土壤TOC含量的提高,秸秆提升效果相对最差,沼渣、菌渣、猪粪、酒渣和秸秆还田处理的0~20cm土壤TOC含量平均增长速率分别为:22.82%、21.88%、16.42%、16.13%和15.57%。     

耕地土壤有机碳组分的季节变化

Carbon fractions in soils apparently vary not only in space, but also over time. A lack of knowledge on the seasonal variability of labile carbon fractions under arable land hampers the reliability and comparability of soil organic carbon(SOC) surveys from different studies. Therefore, we studied the seasonal variability of two SOC fractions, particulate organic matter(POM) and dissolved organic carbon(DOC), under maize cropping: POM was determined as the SOC content in particle-size fractions, and DOC was measured as the water-extractable SOC(WESOC) of air-dried soil. Ammonium, nitrate, and water-extractable nitrogen were measured as potential regulating factors of WESOC formation because carbon and nitrogen cycles in soils are strongly connected. There was a significant annual variation of WESOC(coefficient of variation(CV) = 30%). Temporal variations of SOC in particle-size fractions were smaller than those of WESOC. The stocks of SOC in particle-size fractions decreased with decreasing particle sizes, exhibiting a CV of 20%for the coarse sand-size fraction(250–2 000 μm), of 9% for the fine sand-size fraction(50–250 μm), and of 5% for the silt-size fraction(20–50 μm). The WESOC and SOC in particle-size fractions both peaked in March and reached the minimum in May/June and August, respectively. These results indicate the importance of the time of soil sampling during the course of a year, especially when investigating WESOC.     

长期不同施肥下黑土与灰漠土有机碳储量的变化

采用长期试验,研究了20年不同施肥下1 m深黑土与灰漠土有机碳含量与碳储量的剖面变化。结果表明,单施化肥和不施肥对黑土1 m土层有机碳储量没有显著影响,但灰漠土略有降低。有机肥配施化肥能显著提高土壤有机碳含量和储量。高量有机肥配施化肥（NPKM2）能提高020 cm和2040 cm土层土壤有机碳含量,黑土分别提高56.6%和49.6%、灰漠土提高143.1%和46.9%;常量有机无机配施（NPKM）效果较差,增幅分别为黑土35.1%和35.3%,灰漠土80.2%和4.1%。两种土壤1 m土体的有机碳储量,NPKM2处理分别提高了C 30.7 t/hm2与C 40.6 t/hm2。显然,有机无机肥配施可以显著提高1 m深土体中有机碳储量,主要是由于提高了040 cm土层土壤有机碳含量。     

长期施肥对栗褐土有机碳矿化的影响

【目的】 土壤有机碳矿化是土壤中重要的生物化学过程,与土壤养分的释放、土壤质量的保持以及温室气体的形成密切相关。本文以 25 年长期定位施肥试验为依托,对栗褐土土壤有机碳矿化速率、有机碳累积矿化量的动态变化进行研究,为科学管理土壤肥力、增加栗褐土碳汇、减少温室气体排放提供依据。 【方法】 田间试验开始于 1988,共设置 8 个施肥处理:不施肥 (CK);单施氮肥 (N);氮磷肥合施 (NP);单施低量有机肥(M₁);低量有机肥与氮肥合施 (M₁N);低量有机肥与氮磷肥合施(M₁NP);高量有机肥与氮肥合施 (M₂N);高量有机肥与氮磷肥合施 (M₂NP)。于 2013 年玉米播种前,采集耕层 (0—20 cm) 土壤样品,采用室内培养方法,对土壤碳矿化释放 CO₂ 的数量和速率进行测定,并利用一级动力学方程计算出土壤有机碳库潜在矿化势和周转速率。 【结果】 各肥料处理不同程度地提高了栗褐土总有机碳含量,以高量有机肥与化肥配施作用最为显著。与 CK 相比,M₂N、M₂NP 处理土壤总有机碳含量增加了 121.1%、166.8%。不同处理土壤样品培养有机碳矿化速率均在第一天达到峰值,随后急剧下降。5 d 后,下降趋缓,不同处理 CO₂ 产生速率趋于一致。培养期间,各处理矿化速率变化符合对数函数关系。长期施用不同肥料均可以提高栗褐土有机碳的矿化速率,其大小顺序为:有机肥与化肥配施 > 单施有机肥 > 单施化肥 > 对照。培养 57 d 后,各处理土壤有机碳累积矿化量为 555.0～980.3 mg/kg,以 M₂NP、M₁N 的累积量较高,为对照的 1.77 倍、1.73 倍。长期施肥栗褐土有机碳矿化率呈下降趋势,以处理 M₂NP 下降最明显,与对照相比,降低了 6.3 个百分点。施肥处理土壤的潜在矿化势均高于对照,M₁N、M₂NP 最高,为 923.7 mg/kg 和 926.4 mg/kg,较对照增加了 74.0% 和 74.5%。不同施肥处理均可明显提升土壤有机碳的周转速率,减少周转时间,其中处理 M₁NP、M₂NP 效果最为明显。 【结论】 长期施用化肥、有机肥及有机无机肥配施可有效促进栗褐土有机碳的积累,提高有机碳的矿化速率和周转速率,降低有机碳的矿化率 (累积矿化量占有机碳总量的比率),加强了土壤的固碳能力,以 M₂NP 处理的效果更佳。      

The sensitivity of water extractable soil organic carbon fractions to land use in three soil types

Soil organic carbon (SOC) has a high impact on the sustainability of ecosystems, global environmental processes, soil quality and agriculture. Long-term tillage usually leads to SOC depletion. The purpose of this study was to determine the impact of different land uses on water extractable organic carbon (WEOC) fractions and to evaluate the interaction between the WEOC fractions and other soil properties. Using an extraction procedure at 20°C and 80°C, two fractions were obtained: a cold water extractable organic carbon (CWEOC) and a hot water extractable organic carbon (HWEOC). The results suggest that there is a significant impact from different land uses on WEOC. A lower relative contribution of WEOC in SOC and a lower concentration of labile WEOC fractions are contained in arable soil compared to forestlands. Chernozem soil was characterized by a lower relative contribution of WEOC to the SOC and thus higher SOC stability in contrast to Solonetz and Vertisol soils. Both CWEOC and HWEOC are highly associated with SOC in the silt and clay fraction (<53 µm) and were slightly associated with SOC in the macroaggregate classes. The WEOC fractions were highly and positively correlated with the SOC and mean weight diameter.