A comparison between cutting and animal grazing for dry‐matter yield,quality and tiller density of perennial ryegrass cultivars

Perennial ryegrass (Lolium perenne L.) evaluation trials are often conducted under simulated grazing to identify the most productive cultivars. It is unclear whether simulated grazing identifies the most productive cultivar for animal‐grazed swards. Ten cultivars were established as plots and managed concurrently under simulated grazing (SG), animal grazing (AG) and conservation (CON). The experiment lasted 3 years with dry‐matter (DM) off‐take, digestibility, tiller density and ground‐cover score recorded in all years. A good relationship existed between DM off‐take under SG and CON (R² = 0·73). The relationship between SG and AG was strongest in year 2 and 3 (R² = 0·53 and 0·55 respectively). High DM production was observed in SG swards in year 1; this was weakly related to the DM production of the AG sward. Across the 3 years, the CON treatment had higher yields than either of the other two treatments and was poorly correlated to DM yield under AG, confirming that cultivars should be evaluated under a similar defoliation frequency to their intended use. Tiller density declined quickest under CON and slowest under AG. Some reranking of cultivars occurred between defoliation managements. The results show that simulated grazing is a useful indicator of DM yield performance of animal‐grazed swards.     

Effect of grazing severity on perennial ryegrass herbage production and sward structural characteristics throughout an entire grazing season

The objective of this study, which was part of a larger grazing‐systems experiment, was to investigate the cumulative impact of three levels of grazing intensity on sward production, utilization and structural characteristics. Pastures were grazed by rotational stocking with Holstein–Friesian dairy cows from 10 February to 18 November 2009. Target post‐grazing heights were 4·5 to 5 cm (high; H), 4 to 4·5 cm (intermediate; I) and 3·5 to 4 cm (low; L). Detailed sward measurement were undertaken on 0·08 of each farmlet area. There were no significant treatment differences in herbage accumulated or in herbage harvested [mean 11·3 and 11·2 t dry matter (DM) ha^?1 respectively]. Above the 3·5 cm horizon, H, I and L swards had 0·56, 0·62 and 0·67 of DM as leaf and 0·30, 0·23 and 0·21 of DM as stem respectively. As grazing severity increased, tiller density of grass species other than perennial ryegrass (PRG) decreased (from 3,350 to 2,780 and to 1771 tillers m^?2 for H, I and L paddocks respectively) and the rejected area decreased (from 0·27 to 0·20 and to 0·10 for H, I and L paddocks respectively). These results indicate the importance of grazing management practice on sward structure and quality and endorse the concept of increased grazing severity as a strategy to maintain high‐quality grass throughout the grazing season. The findings are presented in the context of the need for intensive dairy production systems to provide greater quantities of high‐quality pasture over an extended grazing season, in response to policy changes with the abolition of EU milk quotas.     

Identification,ecological evaluation and phylogenetic analysis of non‐symbiotic endophytic fungi colonizing timothy grass and perennial ryegrass grown in adjacent plots

Non‐systemic endophytes coexist with grasses and produce positive or negative effects for the host. In agricultural grasses, endophytes such as Epichloë spp. (formerly: Neotyphodium spp.) enhance the biometric parameters and agricultural value of grass biomass and seeds. Some endophytic fungi produce active substances that exert a negative influence on grass‐fed livestock. There is a general scarcity of studies investigating other endophytic fungi, the species composition of fungal communities, fungal species capable of colonizing different grasses and endophyte transfer between grass taxa. This study aims to fill in the existing knowledge gap by describing the relationships between fungal species and grass species. Timothy grass (Phleum pratense L.) is more readily colonized by endophytic fungi than perennial ryegrass (Lolium perenne L.), and the ratio of fungi isolated from the above species was determined at 3:1. Ecological indicators, especially diversity, were also higher in the fungal community colonizing timothy grass. The vast majority of the isolated fungi were ascomycetes. In addition, two Basidiomycota isolates and three Oomycota isolates (Phythium acanthicum) were also obtained from timothy grass. The most prevalent fungal species were Alternaria alternata, Microdochium bolleyi and Epicoccum nigrum. An analysis of minisatellite DNA regions revealed high levels of genetic polymorphism in A. alternata, whereas the remaining isolates were characterized by low levels of genetic variation or genetic homogeneity. The transfer of endophytic fungi between grass species was determined, which was one of the most important observations made in the study. The Sørensen–Dice coefficient reached 50%, which indicates that all fungal species isolated from perennial ryegrass are capable of colonizing timothy grass.     

Natural diversity in vegetative and reproductive investments of perennial ryegrass is shaped by the climate at the place of origin

In forage grasses, vegetative and reproductive investments are major determinants of yield and persistence. A survey of the diversity of vegetative and reproductive investment traits was carried out on 213 perennial ryegrass genotypes, representing 51 natural European accessions. Plants were phenotyped for traits related to leaf elongation, tillering, reproductive investment and heading date, at two locations with markedly different climates. Strong genetic effects for all traits were found. Interactions between genotype and location were moderate. Plants showed stronger spring leaf growth, lower numbers of tillers and higher reproductive investments at Lusignan (France) than at Melle (Belgium). Plant growth rate and tillering capacity were genetically nearly independent suggesting breeding for both traits simultaneously and independently should be possible. A high genetic diversity was observed for all traits. This diversity was structured in three main clusters. One cluster comprised early‐flowering genotypes with high reproductive investments and high spring growth rates. The remaining genotypes fell into two clusters based on differences in tillering capacity. Clear links were found between traits and the climatic conditions of the region of origin of the accessions. Autumn growth rate was positively correlated with solar radiation at the region of origin in October and February.