The dietary effect of different wheat cultivars for broiler chickens.

1. An experiment was conducted to examine the variability in chemical composition of 16 wheat cultivars and to study how this variability affected the performance of broiler chickens given diets containing 650 or 815 g wheat/kg diet during the first 3 weeks of life. Intestinal viscosity, apparent fat digestibility and apparent metabolisable energy were determined with all diets. Intestinal pH and the relative weight of different parts of the intestine were measured in chickens fed on diets with the high wheat inclusion. 2. The 16 wheat cultivars varied in chemical composition, with protein content ranging from 112 to 127 g/kg dry matter, starch content from 658 to 722 g/kg dry matter and non-starch polysaccharides from 98 to 117 g/kg dry matter. The effect of the different wheat cultivars on the measured parameters was most pronounced in chickens fed on diets with 815 g wheat/kg diet (P<0.001). However, significant (P<0.001) differences between cultivars were still present at the lower wheat content. 3. Milling-quality wheats resulted in better performance (P<0.001) than feeding-quality wheats at the high inclusion, whereas such an effect could not be registered in diets at the lower wheat inclusion. These results indicate that some of the feeding wheats are comparable in quality to milling wheats when included at the concentrations which are more common in practical broiler diets. 4. Decreasing the wheat content in the diets resulted in significantly (P<0.001) better performance and considerably (P<0.001) lower intestinal viscosity (jejunum; 2.4 to 5.9 cps, ileum; 4.6 to 10.9 cps) than in chickens fed on diets with the higher wheat inclusion level (jejunum; 9.1 to 38.4 cps, ileum; 20.8 to 82.0 cps). 5. Diets with 815 g wheat/kg influenced pH in the caeca, but not in the ileum and gizzard. The relative weights of the gizzard and ileum differed significantly (P<0.05) between treatments, whereas those of duodenum, jejunum and caeca were not affected by dietary addition of the different wheat cultivars. 6. The present study demonstrated that the chemical composition and the nutritive value of Danish wheats for broilers vary markedly. The results indicated that the non-starch polysaccharide and especially the arabinoxylan content of wheat are important factors in the discussion of the result obtained. Poor correlations were seen between performance and AMEn and care should be taken when using AMEn values alone to predict the nutritive value of wheat and wheat-based diets.     

Intestinal function and gut microflora of broiler chickens as influenced by cereal grains and microbial enzyme supplementation

A study was conducted to investigate the effect of the key cereal grains and a microbial enzyme supplement on broiler chicken performance, gut microflora and intestinal function. Ingestion of the barley-based diet was associated with low 28-day body weight, decreased feed intake and high FCR. The supplemental enzyme increased feed intake and weight gain of the chickens on a wheat-based diet. The pH of the gizzard and caecal contents varied with the grain type. Enzyme supplementation reduced ileal viscosity, particularly in birds that received the diet based on wheat. The birds on the barley-based diet had lower ileal digestibility of dry matter, protein and energy than those given maize and sorghum-based diets. The ileal digestibility of starch was increased by enzyme supplementation. Enzyme supplementation increased the number of total anaerobic bacteria in the gizzard of birds fed on sorghum and increased lactobacilli in the gizzard of those fed both sorghum and wheat. The birds fed the sorghum-based diet had the lowest counts of caecal total anaerobic bacteria and lactobacilli. Jejunal villus height and villus:crypt ratio of birds fed the barley-based diet were the lowest when compared with those fed the other diets. Enzyme application induced an increase in villus height and villus:crypt ratio of birds on wheat, crypt depth on barley and a reduction in crypt depth of chickens on the sorghum-based diets. The highest activity of maltase and the lowest activity of sucrase were observed in tissue from birds fed on maize and sorghum-based diets respectively. The differences in the performance of broilers on cereal grains could be explained by changes in intestinal morphology, enzyme activities and gut microflora as well as nutrient digestibility. The improved performance by supplemental enzyme in wheat-fed chickens was associated with beneficial changes in intestinal morphology and digesta viscosity.     

Whole blood and plasma viscosity values in normal and ascitic broiler chickens.

1. Whole blood and plasma viscosity values in normal and ascitic broiler chickens were measured. 2. The mean blood viscosity value in ascitic broilers was greater than that of the controls. There was a small but significant difference in the opposite direction between plasma viscosity values of the respective groups of birds. 3. Although the haematocrit and arterial pressure index values in the ascitic birds were raised, there was a fall in the concentration of total plasma protein. 4. The data suggest that the raised viscosity in the ascitic birds was caused by a polycythemia and not by any influence of plasma protein. 5. The cumulative effect of these factors, such as raised blood viscosity values and larger deformed red cells flowing through constricted lung arterioles, may contribute to the pulmonary hypertension and ascites seen in some young commercial broilers.     

The effect of volatile oil mixtures on the performance and ilio-caecal microflora of broiler chickens

1. The aim of this study was to investigate the individual and combined effects of rosemary, oregano and fennel volatile oil (FVO) supplementation on the performance and ilio-caecal bacteriological flora of broiler chickens.

2. A total of 800 male Ross-308 broiler chickens were divided equally into 8 groups; each contained 100 chickens. The study included a control treatment (NC) with no dietary additives that was supplemented with oils according to the following 7 treatments: 200 mg α-tocopherol acetate/kg (PC), 100 mg oregano volatile oil (OVO)/kg, 100 mg rosemary volatile oil (RVO)/kg, 100 mg FVO/kg and an equal mixture of oregano+rosemary+fennel VO (100, 200, 400 mg/kg, VOM-1, VOM-2 and VOM-3, respectively). The experiment lasted for 6 weeks.

3. At the end of the experiment, dietary supplementation with α-tocopherol, oregano, rosemary and FVO and two different volatile oil mixtures (VOMs) (VOM-2; VOM-3) significantly increased the body weights (BWs) of broilers at 7, 14 and 21 d of age compared to the negative control (NC) (?) and VOM-1 groups. At 0–42 d, birds fed on VOM-3 were considerably heavier and also gained more weight than NC (?) and VOM-1 groups.

4. The blend of VOs at 400 mg/kg significantly increased Lactobacillus spp. in faeces. The blends of oregano, rosemary and FVOs (VOM-3) at 400 mg/kg concentration and also VOM-3 group exhibited stronger antibacterial activity against coliform bacteria compared to the NC (?) group.

5. In conclusion, the blend of oregano, rosemary and fennel VOs at higher concentrations (400 mg/kg concentration) in diets can be used to stimulate the growth and can improve the intestinal microbial balance (including a reduction of coliform bacteria and an increase in Lactobacillus spp. counts) of broiler chickens.

     

肉仔鸡肠道微生物的日粮调控——六类饲用抗生素替代品的作用

早在1986年瑞典就率先提出了禁止在肉鸡饲料中使用抗生素,随后抗生素的禁用在世界各地全面展开,自此以来抗生素替代品的研究在动物营养中已受到了越来越多的关注。肠道微生物似乎是饲用抗生素(In-Feed Antibiotics,IFAs)的作用对象,同时抗生素替代品必须有利于维持动物健康和具有一定的促生长作用。本文讨论了六种饲用抗生素替代品对肠道微生物菌群的作用,综述了它们的作用机制和促生长作用,并重点介绍最近几年多位作者以甘露寡糖为抗生素替代品的研究结果。     

肉仔鸡肠道微生物的日粮调控——六类饲用抗生素替代品的作用

1前言在过去数十年,抗生素被用在畜禽日粮中以维持健康和生产效率。但由于致病菌耐药性的产生,抗生素进而威胁公共卫生,所以自1986年瑞     

Pre-caecal digestible phosphorus in maize and wheat for broiler chickens

1. The objective of this study was to determine the coefficient of pre-caecal digestion of P in maize (3.9 g/kg of total P, 0.83 g/kg of phytate P, 138 FTU [phytase units]/kg) and wheat (3.17 g/kg of total P, 1.94 g/kg of phytate P, 666 FTU/kg) in broilers according to the WPSA protocol.

2. For the diets, monosodium phosphate was used as an additional P supplement. Two sets of diets containing 200, 460 and 740 g/kg of wheat or 200, 500 and 740 g/kg of maize were formulated. A total of 288 21-d-old male broilers (Ross 308) were assigned to 24 cages (8 birds per cage) and the 6 test diets were assigned to cages. The coefficient of pre-caecal digestion of P was determined by the indicator method and linear regression.

3. In both ingredients, pre-caecal digestible P increased linearly with increasing inclusion levels of maize or wheat (P < 0.05). The coefficients of digestion of pre-caecal P were estimated to be 0.18 for wheat and 0.33 for maize.     

同源乳酸菌对雏鸡生长性能和盲肠菌群的影响

选择1日龄从肉仔鸡90只,随机分成3组,每组3个重复,每个重复10只鸡,公母各半.对照组饲喂基础目粮,饮用清水;抗生素组饲喂基础日粮+0.02%土霉素,饮用清水;乳酸茵组饲喂基础日粮,饮用清水+同源乳酸茵液[108CFU/(d·只)].试验期21 d.研究结果表明:同源乳酸菌的饲喂能够提高1～3周龄肉仔鸡的平均日增重,降低料肉比,差异显著(P<0.05);与抗生素组相比,添加同源乳酸菌能更好抑制盲肠内大肠杆茵的繁殖,增加乳酸茵的数量.     

Sequential feeding of whole wheat to growing broiler chickens

1. Four broiler feeding trials were performed to examine the suitability of a whole wheat sequential feeding regimen for commercial broiler production. The sequential feeding programme gave a continuous cycle of ad libitum access to only whole wheat followed by the same time of access to only a pelleted diet. The pelleted diet provided a concentration of nutrients to balance that provided by the whole wheat. This was called a balancer diet.

2. The first trial used 144 cage‐reared broilers from 28 to 49 d of age. Four different times of access (4, 8, 12 and 24 h) to the two alternate foods were compared. A whole wheat choice‐feeding treatment and a complete single diet treatment were also compared. Whole wheat accounted for over 40% of the broilers’ total food intakes when they were given the sequential feeding treatments of 8 h or greater. The whole wheat intakes of the birds given the 4 h sequential feeding and the choice‐feeding were only 20 and 5% respectively. There was a non linear relationship between the weight gains of the broilers and the length of the sequential feeding period (P< 0.01). The growth rates of the broilers given sequential feeding were lowest (P< 0.05) in the 4‐h feeding periods but highest (P< 0.05) in the 8‐h periods. Weight gains decreased (P< 0.01) linearly as the sequential feeding periods were increased above 8 h.

3. A second trial, using 144 cage‐reared broilers, examined the effect of different balancer compositions or different wheat varieties in 8‐h sequential feeding. The broilers selected more whole wheat in their diet when they were given balancers with increased cereal contents. However, these broilers did not eat enough whole wheat to compensate for the reduced cereal content of the balancers and their overall diets had lower energy: protein ratios. The two different wheat samples did not result in any differences (P> 0.05) in the proportion of whole wheat selected by the broilers.

4. A third trial compared the diet selections, weight gains, food intakes and water excretions of 72 cage‐reared broilers given whole wheat feeding regimens. The growth rates of the broilers given a loose mix of whole wheat and a pelleted balancer diet were similar (P> 0.05) to broilers given a complete single diet. The growth rates of these two groups were 7% greater (P< 0.05) than broilers given choice‐feeding or 8‐h sequential feeding. There were no differences (P> 0.05) in the total amounts of water excreted among the 4 treatments.

5. The fourth trial used 828 broilers distributed in 6 floor pens. They were given either a complete diet or an 8‐h whole wheat sequential feeding regimen from 25 to 46 d of age. The food intakes and the growth rates of the broilers were reduced (P<0–05) in die sequential feeding treatment by 9% and 5% respectively. There was no difference (P> 005) in litter moisture between the 2 treatments.     

Effect of different concentrations of Bacillus subtilis on growth performance, carcase quality, gut microflora and immune response of broiler chickens

1. An experiment was conducted to study the effects of different Bacillus subtilis concentrations on productivity, carcase quality, immune response and the gut microflora of broiler chickens. 2. There were 5 treatment groups: control, with no added Bacillus subtilis supplementation; and 4 treatment groups receiving feed supplemented with different concentrations of B. subtilis. 3. Weight gain was significantly higher and the feed conversion rate was significantly better in all groups receiving feed supplemented with B. subtilis, regardless of its concentration. The groups given B. subtilis supplementation produced relatively bigger breasts, and smaller carcases and thighs, compared with the control group. 4. The appearance of increased diffuse lymphohistiocytic infiltration and solitary lymphoid follicles in the mucosa, and a stronger response to NDV vaccination, indicate increased immunological responses in chickens fed with the B. subtilis supplemented diet. 5. The higher inclusion rate of B. subtilis did not increase Lactobacillus concentrations in the ileum or in the caecum, but decreased the E. coli population significantly.     

Delivery routes for probiotics: Effects on broiler performance,intestinal morphology and gut microflora

Four delivery routes, via, feed, water, litter and oral gavage, were examined for their efficacy in delivering a novel probiotic of poultry origin, Lactobacillus johnsonii, to broilers. Seven treatments of 6 replicates each were allocated using 336 one-day-old Cobb broiler chicks. The treatments consisted of a basal diet with the probiotic candidate, L. johnsonii, added to the feed, and three treatments with L. johnsonii added to the drinking water, sprayed on the litter, or gavaged orally. In addition, a positive control treatment received the basal diet supplemented with zinc-bacitracin(Zn B, 50 mg/kg). The probiotic strain of L.johnsonii was detected in the ileum of the chicks for all four delivery routes. However, the addition of L.johnsonii as a probiotic candidate did not improve body weight gain, feed intake and feed conversion ratio of broiler chickens raised on litter during the 5-week experimental period regardless of the route of administration. The probiotic treatments, regardless of the routes of delivery, affected(P 0.05) the pH of the caecal digesta and tended(P = 0.06) to affect the p H of the ileal digesta on d 7, but the effect disappeared as the birds grew older. All probiotic treatments reduced the number of Enterobacteria in the caeca on d 21, and tended(P 0.054) to reduce it in the ileum and caeca on d 7 and in the ileum on d 21 compared with the controls. The probiotic also tended to increase the number of lactic acid bacteria and lactobacilli in the ileum and caeca on d 7, but this trend was not evident on d 21. The trend appeared most pronounced when the probiotic was delivered orally or via litter. The probiotic also decreased(P 0.05) the population of Clostridium perfringens rapidly from an early age to d 21 in the caeca, leading to a3-fold decrease in the number of C. perfringens between d 7 and 21. It also showed that the probiotic treatment presented the lowest number of C. perfringens in the caeca. Delivery of the probiotic through feed, water and litter increased(P 0.01) the weight of the pancreas on d 21, but the probiotic did not affect other morphometric parameters of the gut. Furthermore, the probiotic did not affect the p H and the concentrations of short chain fatty acids and lactic acid in either the ileum or caeca.     

The effect of continuous ultraviolet irradiation on broiler chickens.

1.Continuous ultraviolet irradiation (10.45 muW/cm2) did not affect the growth or food conversion efficiency of broilers. 2. The cornea of the eye was roughened by the treatment and there was a loss of the regular arrangement of the corneal cells: vision was not significantly affected after 49 d exposure.     

Characterisation and response of intestinal microflora and mucins to manno-oligosaccharide and antibiotic supplementation in broiler chickens

1. An experiment was conducted to characterise and evaluate, in comparison to zinc bacitracin (ZnB), the response of intestinal microflora and mucins to manno-oligosacchares (MOS, Bio-MOS®, Alltech Biotechnology, Nicholasville, Kentucky, USA).

2. Supplementation of MOS and ZnB selectively increased the intensity of sulphomucins. As revealed by the plate culture method, MOS accelerated the maturation of gut microflora by promoting the growth of lactobacilli in the ileal mucosa and vice versa on ileal and caecal clostridia. Unlike MOS, ZnB suppressed the growth of intestinal bacteria, especially those of lactobacilli and clostridia. Use of T-RFLP further revealed that MOS increased the diversity of lactobacilli in the ileum and ileal mucosa but the opposite was observed for ZnB. It also appears that MOS and ZnB possessed a common property in differentially favouring the growth of certain Lactobacillus species. There was also evidence to show that both MOS and ZnB also increased the homogeneity of the gut microflora, possibly through the regulation of the overall gut bacterial communities.

3. Improvement in intestinal microbial homogeneity and mucin synthesis, coupled with the differential selections for certain specific beneficial bacterial species, may ultimately be proven to be the target mechanisms in the search for more effective alternatives to antibiotics.     

Soft wheat instead of hard wheat in pelleted diets results in high starch digestibility in broiler chickens

(1) The aim of the experiment was to re-examine variations in digestibilities of food components in 3-week-old broiler chickens fed on pelleted diets based on wheats differing in lipase activity and hardness. Fourteen wheat (Triticum aestivum) samples, each from a different cultivar, were included at 550 g/kg in 14 different diets given to male Ross broiler chicks from 7 d of age. The other main ingredients consisted of soyabean meal (353 g/kg) and rapeseed oil (55 g/kg). A 15th diet containing durum wheat (Triticum durum) was also tested. (2) Hardness of wheats (Triticum aestivum) varied between 14 (very soft) and 88 (very hard), and lipase activity of wheats varied from 1 to 13.1 (relative scale). No significant correlation was found between in vitro viscosities and other parameters such as hardness, particle size of wheat flours and lipase. Hardness was correlated with the mean particle size of wheat flours and durability of pellets. (3) Individual lipid digestibilities were negatively correlated with in vitro viscosities of wheats. (4) Individual starch digestibilities were negatively correlated with wheat hardness, particle size of wheat flour before pelleting, and pellet durability. The ratio of measured AME(N) to predicted AME(N) was also negatively correlated with wheat hardness. Simple regression calculation showed that a 100-point increase in wheat hardness resulted in a 3% decrease in the AME(N) value of diets. Multiple regression calculation showed the food/gain ratio (d 10 to d 21) to be positively related to wheat hardness and negatively related to pellet durability. (5) Wheat lipase activity was positively correlated with individual starch digestibility, which was the reverse of a result obtained in a previous experiment. Thus, wheat lipase activity did not seem consistent for predicting starch digestibility and AME(N) values. (6) Among all wheat samples, durum wheat showed the highest protein content and the lowest content of water-insoluble cell-wall. Starch digestibility of durum wheat tended to be lower than that of other wheats (0.916 vs 0.936). However, no significant difference in AME(N) was observed between the durum wheat sample and other wheats. (7) Gut morphometric data measured at d 24 did not show significant differences between dietary treatments.     

The effect of conditioning temperature and exogenous xylanase addition on the viscosity of wheat-based diets and the performance of broiler chickens

1. A total of 1920 Ross broiler chicks were used in a 6 treatment by 8 replicate growth study to investigate the effect of conditioning temperature and exogenous xylanase addition on diet characteristics and bird performance. 2. Increasing conditioning temperature from 80 to 90 degrees C increased the viscosity of the diets by approximately 6 mPa.s and reduced body weight gain of the broiler chickens by 7%. 3. Furthermore, there was a significant interaction between temperature and the addition of exogenous xylanase, with a proportionately greater positive response to added xylanase in the diets that were pelleted at higher temperatures. 4. Addition of exogenous xylanase also increased the proportion of soluble arabinoxylan in the feed and reduced dietary viscosity, which is likely to be related to the degree of polymerisation of the soluble carbohydrate. 5. These results suggest that conditioning temperature should be considered as a factor when attempting to predict the response of broilers to added xylanase, especially when high-viscosity or 'low-quality' wheat is being used. 6. It can be concluded that pelleting wheat-based diets above 80 degrees C can compromise bird performance and that, if higher temperatures are to be employed, the use of exogenous xylanase is critical to maintain productivity.     

Chemical composition and the nutritive quality of different wheat cultivars for broiler chickens

1. Different wheat cultivar samples were grown in replicated trials on a single site in three harvest years. A total of 23 wheat samples were harvested. Nutritionally complete, meal-form diets that included each wheat sample at 650 g/kg were used to compare broiler growth performance and determine apparent metabolisable energy (AME). The relationships were examined between these variables of nutritive value for broilers and the chemical composition and tests of quality on the wheat samples. 2. The total starch contents of the wheat samples ranged from 594 to 732 g/kg dry matter (DM). The mean total non-starch polysaccharide (NSP) content was 100g/kg DM comprising 73.5 g/kg DM of insoluble material. Endosperm hardness ranged between 10 and 77 relative units and Beaver was the softest cultivar in two of the batches of samples. The AME of the wheat-based diets had a maximum range of 0.5 MJ/kg within each of the three batches of wheat that were tested. 3. Endosperm hardness of the wheat grains, within a harvest year, was positively correlated with broiler weight gain and the 1000-grain weight was negatively correlated with feed conversion efficiency (FCE). Starch content and gross energy were positively correlated with determined AME, and DM, 1000-grain weight and water holding capacity were negatively correlated with AME. 4. A step-wise regression technique indicated that endosperm hardness and ash content of the wheat were the explanatory variables that, within harvest years, significantly reduced the unexplained variation in broiler growth rate, feed intake and FCE. The contents of total starch, crude protein and ether extract were the explanatory variables that, within harvest years, significantly reduced the unexplained variation in AME.     

Influence of dietary mushroom Agaricus bisporus on intestinal morphology and microflora composition in broiler chickens

In this study, we evaluated the intestinal morphology and bacteria populations in broiler chickens fed for six weeks diets that contained different amount of the mushroom Agaricus bisporus. Ninety day-old female chicks were randomly divided into three dietary treatments, each with three replicates kept in floor pens and fed a basal diet supplemented with the dried mushroom at levels of 0, 10 or 20 g/kg fresh feed. Feed and water were offered to birds ad libitum. The morphological examinations of the intestine were carried out on 1-cm long excised segments from duodenum, jejunum and ileum. The populations of total aerobes, total anaerobes, Lactobacilli spp., Bifidobacteria spp., Escherichiacoli, Bacteroides spp. and Enterococci were enumerated in ileum and caecum by conventional microbiological techniques using selective agar media. The results of the study showed that dietary mushroom supplementation did not significantly affect intestinal morphology at either level of inclusion. Morphometrical parameters of depth of duodenum, jejunum and ileum crypt and height of villi revealed no differences amongst dietary treatments. In the ileum, Lactobacilli spp. were higher in birds supplemented at the level of 20 g/kg compared to controls; however, other measurements of bacteria loads were similar amongst the three dietary treatments. In the caecum, Lactobacilli spp. and Bifidobacteria spp. loads were higher in birds supplemented at either level of inclusion compared to control birds, although these did not differ between the two levels of supplementation. In conclusion, dietary mushroom supplementation may beneficially affect intestinal health of broiler chickens.