The effectiveness of seed priming and foliar application of zinc- amino acid chelates in comparison with zinc sulfate on yield and grain nutritional quality of common bean

Abstract

The efficacy of seed priming and foliar application of zinc-amino acid chelates including zinc-histidine [Zn(His)₂] and zinc-methionine [Zn(Met)₂] in comparison with zinc sulfate (ZnSO₄) on yield and grain nutritional quality of two common bean cultivars (Phaseolus vulgaris L., cvs Talash and Sadri) was investigated in a severely Zn-deficient calcareous soil (DTPA-Zn: 0.38?mg kg^?1 soil) in a pot experiment. Bean response to Zn application varied depending on the Zn fertilizer, application method and cultivar. In ‘Talash’, seed priming with [Zn(His)₂] and [Zn(Met)₂] led to 24.1 and 11.6% increase in the grain yield of bean in comparison with ZnSO₄ treatment, respectively. In both cultivars, foliar application of [Zn(His)₂] led to significant increase in the grain yield in comparison with ZnSO₄. The highest grain Zn concentration was obtained by seed priming with [Zn(Met)₂] in ‘Sadri’ and [Zn(His)₂] in ‘Talash’, respectively. For Zn-amino acid chelates, seed priming was more effective than foliar application in increasing grain yield and Zn concentration. Foliar application of [Zn(His)₂] and [Zn(Met)₂] in ‘Sadri’ and [Zn(Met)₂] in ‘Talash’ resulted in higher protein content in bean grain as compared with ZnSO₄. In both cultivars, foliar application of [Zn(Met)₂] was the more effective than seed priming to increase grain protein content. The highest water-soluble carbohydrates concentration of grain was obtained by seed priming with [Zn(Met)₂] and [Zn(His)₂] in ‘Sadri’ and ‘Talash’ cultivars, respectively. Therefore, seed priming with [Zn(His)₂] and ZnSO₄ in ‘Sadri’ and [Zn(Met)₂] in ‘Talash’ can effectively be used for improving yield of common bean in Zn-deficient calcareous soils.     

Effects of foliar applied zinc in the form of ZnSO4 and Zn-amino acid complexes on pistachio nut yield and quality

Abstract

Despite the importance of Zn nutrition, little information is available on the effectiveness of foliar Zn application, in general, and Zn-amino acid complexes in particular on pistachio nut yield and quality. Response of pistachio (Pistachio vera L. cv. Akbari) to foliar application of zinc (Zn) in the form of mineral (ZnSO₄) and complexed with methionine (ZnMet), lysine (ZnLys), and lysine plus methionine (ZnLysMet) was investigated. Lysine alone treatment was used to distinguish the effects of accompanying Lys. A control treatment with no amino acids and Zn was also used. The critical deficiency concentration of Zn in the leaf to achieve the highest 100-kernel mass was 37.6?mg kg^?1. Foliar application of Zn significantly increased leaf Zn concentration in comparison with control. In both years, foliar supply of Zn in the form of complexed with amino acids resulted in a significant increase of total nut yield, fresh mass of 100-nut, and spilled nuts. A significant decrease in the empty nuts was also observed by Zn application. The effectiveness of ZnMet in improving yield and quality attributes of pistachio was in general, higher than the other Zn sources.     

硫酸锌和EDTA-Zn不同施用方法对第二季小麦籽粒锌和土壤锌有效性的影响

【目的】在潜在缺锌石灰性土壤上,特别是种植小麦并以此为主粮的地区,缺锌问题日益受到人们的关注。提高小麦籽粒锌含量以满足人体锌需求,对于改善人体锌营养不良的现状具有重要意义。【方法】以ZnSO₄和Zn-EDTA为锌源,布置了2个为期两年的田间定位试验。试验均采用裂区设计,即主因子为喷施锌肥,设喷施与不喷2个主处理;副因子为土施方法,设不施锌、均施、条施3个副处理。在第1季试验基础上,第2季不再土施锌肥,调查了小麦籽粒锌含量、土壤有效锌含量及锌组分含量,分析了第1季锌肥的后效。【结果】第2季单独喷施ZnSO₄小麦籽粒Zn含量提高了11.13 mg/kg,提高幅度为33%,而喷Zn-EDTA无明显效果。不喷Zn时,第1季均施和条施的ZnSO₄在第2季均表现出一定后效,小麦籽粒锌含量比对照分别提高了6.05、3.51 mg/kg,提高幅度为20%和11%;喷Zn时,第2季均施和条施ZnSO₄处理的小麦籽粒锌含量增加了28.59和21.59 mg/kg,增幅100%和76%,表现出显著富锌作用,但增加幅度比单独喷施要小很多。第1季土施的两种锌肥在第2季小麦收获后DTPA-Zn仍维持在1 mg/kg以上,即不喷Zn时,均施和条施ZnSO₄处理的土壤有效锌含量分别为1.99和1.65 mg/kg,均施和条施Zn-EDTA的有效锌含量分别为1.23和1.01 mg/kg;喷Zn时,均施和条施ZnSO₄处理的土壤有效锌含量分别为1.44和2.22 mg/kg,均施和条施Zn-EDTA处理的有效锌含量分别为1.16和1.10 mg/kg。土壤各锌组分含量均表现为:松结有机态Zn > 碳酸盐结合态Zn > 氧化锰结合态Zn > 紧结有机态Zn > 交换态Zn。具体而言,第1季均施和条施ZnSO₄,第2季结束后交换态Zn（Ex-Zn）、松结有机态Zn（Wbo-Zn）、碳酸盐结合态Zn（Car-Zn）含量均显著提高,其提高幅度分别为184%和116%;75%和85%;53%和43%。而均施和条施Zn-EDTA仅Ex-Zn、Wbo-Zn含量显著提高,其提高幅度分别为232%和132%;18%和10%。均施Zn-EDTA处理的锌肥利用率为0.27%,条施为0.70%,后者约为前者的3倍;而条施与均施ZnSO₄无差异。【结论】在潜在缺锌石灰性土壤上,单独喷施ZnSO₄显著提高了小麦籽粒锌含量,而喷施Zn-EDTA效果不显著;土施ZnSO₄和Zn-EDTA,不论条施或均施,虽然会使有效锌（DTPA-Zn）及较高活性锌形态（Ex-Zn、Wbo-Zn）长时间维持较高含量,但对第2季小麦籽粒富锌的后效有限;土施基础上配合喷施ZnSO₄对小麦籽粒锌的含量效果最令人满意。     

Phytic acid,iron and zinc content in wheat ploidy levels and amphiploids: the impact of genotype and planting seasons

Micronutrient deficiency is one of the most common and widespread nutritional issues. Among the factors mitigating the bioavailability of Zn (zinc) and Fe (iron), phytic acid plays a key role; therefore, in order to scrutinize genetic alterations ?related to micronutrient and phytate contents, we examined the concentrations of zinc, iron, and phytic acid, as well as its mole ratio to ?zinc in various wheat species grown in two planting seasons. The concentrations of phytic acid and its mole ratio to zinc were 0.61?1.55 g kg^?1 dry weight and 1.88?4.17 for autumn, and 0.97?2.02 g kg^?1 dry weight and 2.10?4.05 for spring planting. There was a significant discrepancy among wheat species; tritipyrum had the highest concentration of iron, phytic acid and its mole ratio to zinc, and T. monococcum and T. aestivum recorded reasonable zinc bioavailability. Correlation studies between grain phytic acid concentrations and other measured traits revealed various relationships, denoting an irrefutable impact of planting season and wheat ploidy levels on modification of wheat genotypes. The characters contributing more positively with principal component (PC) 1 were Zn and Fe under spring planting and Fe under autumn planting. Spike number per square meter, biological yield and grain yield in spring cultivation, and grain zinc concentration in autumn cultivation were positively correlated to principal component (PC) 2. Given that the concentration of Fe and Zn in all the studied genotypes is relatively high and due to the existence of other desirable agronomic traits, this study believes that it could possibly enhance the applicability of some of these genotypes for breeding purposes.     

Potential of zinc seed treatment in improving stand establishment,phenology, yield and grain biofortification of wheat

Abstract

One of the problems in obtaining high wheat yield is the unavailability of micronutrients in balanced quantities. Zinc is an essential micronutrient due to its involvement in many metabolic processes in plant. In this experiment, seeds of two wheat cultivars (Faisalabad-2008 and Lasani-2008) were subjected to soak in aerated Zn solution of 0.1 and 0.01?M for 12?hr. For the seed coating, Zn was adhered to the wheat seeds by using Arabic gum by using zinc sulfate (ZnSO₄·7H₂O) as a source. Untreated dry seeds were considered as a control. Results indicated that field emergence was improved by Zn seed treatments, maximum numbers of seedlings were observed in seed priming with 0.01?M Zn solution. Seed osmoprimed with 0.01?M Zn solution improved the grain yield, biological yield, and other yield related traits. Grain and straw Zn enrichment were also enhanced in seed osmoprimed with 0.01?M Zn solution.     

锌腐酸肥料对冬小麦群体、产量及品质的影响

通过大田微喷灌节水灌溉方式研究锌腐酸肥料对冬小麦群体、产量及品质的影响。采用磷肥种类为锌腐酸磷酸二铵和普通磷酸二铵;氮肥种类为锌腐酸尿素和普通尿素;不施肥为对照。结果表明:底施普通尿素和磷酸二铵处理的拔节期总茎数和单株茎数均较高;底施普通尿素和磷酸二铵,拔节期追施锌腐酸尿素产量最高,达6 730.5 kg/hm~2。拔节期追施锌腐酸尿素处理的籽粒产量均高于追施普通尿素,增产主要与成穗数和穗粒数增加有关;底施锌腐酸尿素和锌腐酸磷酸二铵,追施锌腐酸尿素的小麦籽粒品质指标最高,拔节期追施锌腐酸尿素的提质效果优于普通尿素。     

铜氮配施对冬小麦籽粒产量和蛋白质含量的影响

在豫中潮土区通过田间裂区试验研究了铜、氮配施对冬小麦籽粒产量和蛋白质的影响。结果表明,氮肥对冬小麦籽粒产量和蛋白质产量影响达极显著水平,对蛋白质含量影响不显著。铜肥对籽粒产量和蛋白质产量的影响都达到极显著水平,对籽粒蛋白质含量影响达显著水平。氮、铜交互对冬小麦籽粒产量和蛋白质产量影响达极显著差异,对蛋白质含量影响也达显著差异。综合考虑产量和蛋白质因素,本试验条件下,铜、氮配施以N1Cu2处理(即施氮120 kg.hm-2,施铜45 kg.hm-2)为适宜的铜、氮配施比例。     

氮素和水分处理对稻米植酸含量和蛋白组分的影响

以直立密穗型粳稻品种秀水110和弯曲散穗型粳稻品种春江15为材料,对不同氮素水平处理和水分管理方式下稻米植酸含量和蛋白组分的影响及其互作效应进行了研究。结果表明,旱作栽培处理会导致水稻籽粒中植酸含量上升,而施氮处理对籽粒植酸含量的影响效应与水稻的水分管理方式有关,在常规水作条件下,高氮处理（N3）的籽粒植酸含量有所提高,但在旱作栽培方式下,中氮（N2）和低氮处理（N1,不施N）的籽粒植酸含量却略高于高氮处理（N3）,氮素水平与水分管理方式间的互作效应明显; 水稻籽粒植酸含量与粗蛋白总量、4种蛋白组分（清蛋白、球蛋白、醇溶蛋白和谷蛋白）及有关产量性状指标（有效穗数、每穗粒数、千粒重和结实率）在不同水肥处理间的相关性不显著,但氮肥用量过高不仅会导致水稻的产量水平下降,而且也不利于稻米营养品质的改良; 水肥处理对水稻籽粒植酸含量、蛋白总量和4种蛋白组分穗内粒位分布也存在一定影响,着生在稻穗下部的弱势粒,其稻米植酸含量高于稻穗上部或中部的强势粒,因此改善弱势粒灌浆的水肥管理措施将有利于稻米植酸含量的降低。     

Predicting grain yield and protein content in winter wheat at different N supply levels using canopy reflectance spectra

A field experiment using a split-plot randomized complete block design with three replications was carried out to determine relationships between spectral indices and wheat grain yield （GY）, to compare the performance of four vegetation indices （VIs） for GY prediction, and to study the feasibility of VI to estimate grain protein content （GPC） in winter wheat. Two typical winter wheat （Triticum aestivum L.） cultivars ＇Xuzhou 26＇ （high protein content） and ＇Huaimai 18＇ （low protein content） were used as the main plot treatments and four N rates, i.e., 0, 120, 210, and 300 kg N ha^-1, as the sub-plot treatments. Increasing soil N supply significantly increased GY and GPC （P ≤ 0.05）. For the two cultivars combined, significant and positive correlations were found between four VIs and GY, with the strongest relationship observed when using the green ratio vegetation index （GRVI） at mid-filling. Cumulative VI estimates improved yield predictions substantially, with the best interval being heading to maturity stage. Similar results were found between VI and grain protein yield. However, when using cumulative VI, GPC showed no significant improvement. The strong relationship between leaf N status and GPC （R2 =0.9144 for ＇Xuzhou 26＇ and R2 = 0.8285 for ＇Huaimai 18＇） indicated that canopy spectra could be used to predict GPC. The strong fit between estimated and observed GPC （R2 = 0.7939） indicated that remote sensing techniques were potentially useful predictors of grain protein content and quality in wheat.     

离体培养下锌对春小麦子粒形成及干物质积累的影响

采用离体穗培养技术研究了锌对春小麦后期生长及产量的影响。结果表明,在Zn2+浓度为6.mmol/L的处理小麦的生长发育状况最好,其旗叶内叶绿素含量、倒一节长度、穗粒数、结实率、千粒重以及穗的干重与对照相比差异均达到显著或极显著水平;倒二节长度和小花数与对照的差异不显著。低锌和锌过量(Zn2+浓度为0.3和300mmol/L)条件下,小麦的生长发育受阻,各项性状指标均低于对照,不利于产量的提高;而且锌过量不但降低产量还造成锌肥的浪费和环境污染。     

不同施硫量对冬小麦光合特性和产量的影响

以8901-11和4185两个冬小麦品种为材料,于2001～2002年度在河北农业大学教学基地进行了试验,研究了不同施硫水平对小麦光合特性和产量的影响。试验设4个施硫量处理,分别为S0、30、60、90kg/hm2,采用裂区设计,3次重复。试验结果表明,在一定的供硫范围内(0～60kg/hm2),顶部功能叶在各生育时期,倒3叶和旗叶在展开到衰亡过程中的叶绿素含量增加,光合速率提高,可溶性蛋白质含量增加。在该施硫量范围内,两品种的产量均随着施硫量的增加而增加,且以60kg/hm2的施硫量水平产量最高,单位面积穗数、每穗粒数和千粒重随施硫量的变化趋势也同产量的变化趋势基本一致。     

磷素子粒生产效率不同品种的小麦磷素吸收利用差异

盆栽试验研究了130份小麦不同生育时期的干物重、磷素含量、子粒产量等指标,采用组内最小平方和的动态聚类方法将供试品种按磷素子粒生产效率从低到高依次分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ和Ⅵ 6个类型,研究不同类型磷素吸收利用的差异。结果表明: 1）供试品种的磷素子粒生产效率差异较大（CV=1660%）,Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ和Ⅵ类品种的平均磷素子粒生产效率为P 13629、15167、16916、18589、20132、24466 g/g。子粒产量随磷素子粒生产效率提高呈增加趋势（r=03203**）。2）不同生育时期,小麦植株磷浓度与吸磷量类型间差异显著或极显著。成熟期磷素子粒生产效率与植株磷浓度极显著正相关（r=06969**）,子粒产量与抽穗期、成熟期植株吸磷量显著或极显著相关（r=02966*、r=09271**）。3）不同生育时期磷素干物质生产效率的类间差异均达显著水平; 成熟期磷素干物质生产效率与磷素子粒生产效率极显著正相关 （r=07391**）。4）拔节期、抽穗期和成熟期干物重均表现出随磷素子粒生产效率增加而增加的趋势,成熟期尤为突出。拔节期成熟期磷素吸收量是影响子粒产量形成的重要因素,磷素子粒生产效率高的品种在拔节期后有较强干物质和子粒产量形成能力。     

施氮量和底追比例对小麦氮素吸收利用及子粒产量和蛋白质含量的影响

在大田栽培条件下,运用15N示踪技术研究了不同施氮量和底追肥比例对小麦氮素利用和子粒产量及蛋白质含量的影响。结果表明,施用氮肥提高了小麦植株的氮素积累量、子粒产量、蛋白质含量和蛋白质产量。相同施氮量条件下增加追肥氮的比例,提高了氮肥农学利用率和吸收利用率,增加了植株地上部器官(子粒+营养器官)中追肥氮、土壤氮的积累量,提高了营养器官中氮素的转运量和开花后氮素的同化量,增加了子粒蛋白质含量。相同的氮素底追肥比例条件下,将240.kg/hm2施氮量降至168.kg/hm2的处理,氮肥农学利用率、氮肥吸收利用率、氮肥偏生产力提高,子粒中土壤氮的积累量增加,植株地上部器官中土壤氮的积累量亦增加,开花后氮素同化量提高,子粒蛋白质含量增加。各施氮处理间子粒产量无显著差异。在本试验条件下,施氮量为168.kg/hm2且全部于拔节期追施是兼顾产量、品质和效益的优化处理。     

硅锌硼配施对红壤区双季稻产量和群体发育特征的影响

水稻是典型的喜锌、喜硅和低度需硼作物,而我国红壤区土壤中硅、硼、锌等主要中微量元素含量普遍偏低,施用硅、锌、硼对水稻生产存在重大影响。以早稻品种中嘉早17和晚稻品种H优518为试验材料,研究硅、锌、硼配施对红壤区双季稻产量和群体发育特征的影响。结果表明:与CK处理相比,早稻季Si+Zn和Si+Zn+B处理产量分别显著增加8.6%和12.6%,晚稻季仅Si+Zn+B处理产量显著增加10.6%;早稻季Si+Zn+B处理有效穗数、结实率和千粒重均显著高于CK处理(P0.05),晚稻季仅Si+Zn和Si+Zn+B处理有效穗数显著增加;早稻和晚稻群体总颖花量与产量之间呈极显著线性正相关关系(P0.001)。与CK处理相比,增施硅、锌、硼后各生育期分蘖数、叶面积指数、SPAD值、生物量积累均有增加的趋势,其中Si+Zn+B处理增加幅度最大。除早稻季Si处理外,早稻季和晚稻季增施硅、锌、硼肥后各处理地上部群体氮素吸收总量均显著高于对照,且Si+Zn+B处理显著高于其他处理(P0.05)。因此,在红壤区双季稻生产中应重视硅、锌、硼等中微量元素的平衡施用,尤其是温度条件较低的早稻季更应重视这些元素的施用。     

氮肥运筹比例对稻田套播强筋小麦子粒品质和产量的影响

为探索稻田套播强筋小麦实现优质高产的适宜氮肥运筹比例,选用强筋小麦品种烟农19,于2004至2006年在江苏淮北的铜山和赣榆两地研究了5种氮肥运筹比例(基肥∶分蘖肥∶拔节肥∶孕穗肥分别为6∶1∶3∶0、5∶1∶4∶0、5∶1∶2∶2、3∶3∶2∶2和3∶3∶1∶3)对稻茬强筋小麦子粒产量和营养品质与加工品质的影响。结果表明,随拔节肥和孕穗肥施氮比例上升:1)子粒蛋白质和湿面筋含量上升,但对清蛋白与球蛋白含量影响较小,对醇溶蛋白、谷蛋白含量影响较大;2)子粒淀粉含量下降,面粉峰值粘度、低谷粘度、稀解值和最终粘度呈先下降后又上升的趋势;3)面团形成时间和稳定时间增加;4)子粒产量逐渐升高,但后期施肥比例过高,产量有所下降。因此,稻田套播强筋小麦优质高产栽培氮肥运筹宜采用基肥∶分蘖肥∶拔节肥∶孕穗肥为3∶3∶2∶2的比例。     

深耕改善砂姜黑土理化性状提高小麦产量

为探明砂姜黑土农田适宜的耕作方式,进一步挖掘砂姜黑土生产潜力,发挥地域资源优势,以周麦27为试验材料,在大田条件下设置免耕、旋耕(15 cm)、深耕(30 cm)3种耕作方式,研究了耕作方式对砂姜黑土农田土壤容重、有机碳含量、无机氮含量以及小麦籽粒产量的影响。结果表明,在小麦苗期和成熟期,3种耕作方式处理间0~10 cm土层土壤容重差异不显著(P0.05),但深耕处理显著降低10~30 cm土层土壤容重(P0.05)。在小麦苗期、越冬期、拔节期、开花期和成熟期,3种耕作方式对0~20 cm土层土壤有机碳含量的影响规律不明显,但深耕处理明显增加20~40 cm土层土壤有机碳含量;20~40 cm土层土壤铵态氮含量均为深耕旋耕免耕。与免耕处理相比,深耕处理通过增加小麦穗粒数和千粒质量,最终促使籽粒产量增加16.33%。综上所述,在该试验条件下,在秸秆还田的基础上,小麦季30 cm深耕处理可以降低土壤容重,增加土壤有机碳含量,进而提高小麦籽粒产量,可作为砂姜黑土农田适宜的耕作方式。     

Effects of seed priming with plant growth-promoting rhizobacteria on wheat yield and soil properties under contrasting soils

In the present investigation, different strains of Plant growth-promoting rhizobacteria (PGPR), namely Bacillus megaterium, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their growth-promoting effects on wheat as well as on soil properties under field conditions at two different sites having sandy loam and silt loam type of soils. PGPR strains were evaluated either singly or in consortia. Amongst all the treatments, wheat inoculated with consortia was found most effective as it increased grain yield up to 53% over control in silt loam soil, whereas, corresponding effects in sandy loam soil were less pronounced as an increase of 31% was observed in corresponding treatments, respectively. Enhanced effects on soil properties were also more intense in silt loam as there was an increase of 205% organic matter as against sandy loam soil where this value was 110%.     

Effects of sulphur application on grain yield and quality,and assessment of sulphur status in winter wheat (Triticum aestivum L.)

Abstract

Winter wheat was grown in 26 field trials in Norway during the period 2004–2006. The main aim was to determine the effect of various sulphur (S) fertilization strategies at two different nitrogen (N) levels on grain yield and quality of winter wheat. With the exception of four trials in central Norway, all the trials were located in the south-eastern part of the country.

Increasing the N fertilization from 170 to 210 kg N ha^?1 resulted in significantly higher grain protein content (GPC) and sodium dodecyl sulphate (SDS) sedimentation volume. On clayey soils in southeast Norway, increased N-fertilization also resulted in higher grain yield and lower test weight and thousand grain weight (tgw).

Omitting S fertilization significantly reduced grain yields on sandy soils in southeast Norway and on loam and silt loam in central Norway, whereas no yield reduction was found on clay soils. SDS sedimentation volume and specific SDS (sSDS = SDS/GPC) were significantly reduced when S-fertilization was omitted. GPC was reduced by S fertilization in central Norway. Generally there were no significant responses at application rates higher than 12 kg S ha^?1.

The malate:sulphate method is a field-based diagnostic test used to determine whether S deficiency is likely to occur. The results did not indicate that this test is reliable under Norwegian conditions. On the other hand, the use of a chlorophyll meter measurements to assess S status gave promising results.     

Amino acid content and composition of winter wheat affected by Nitrogen Fertilization

The protein quality of wheat is closely correlated with its amino composition. Consequently, when conducting a N fertilization experiment, it is important to know how the ratio of essential amino acids determining the nutritional quality, especially that of the most important limiting amino acids of wheat within the increased protein content, works out. The effect of nitrogen fertilizer applied at different times and/or doses (0–200 kg/ha) was investigated on grain protein and amino acid contents and yields of a Hungarian bread wheat variety on a soil type of medium ? status (eutric cambisol) in Western Hungary for three years. 160 kg N/ha was necessary to reach the highest possible crude protein content and the split application of this dose proved to be the most beneficial to the crude protein yield on the three years’ average. Though most of the treatments seemed to be disadvantageous from the point of view of amino acid composition considering the nutritional quality, the amount of essential amino acids, especially that of lysine harvested per hectare showed a considerable increase (up to 28.4%).     

低锌旱地土壤水分对小麦产量和锌利用的影响

【目的】西北旱地土壤有机质含量低,pH和碳酸钙含量高,导致土壤有效锌含量低,加之水分缺乏,不仅制约冬小麦生长和产量,还严重影响小麦锌的吸收利用。本研究选取西北旱地典型缺锌区,在土施锌肥的基础上,设置了2年的补充灌水田间试验,进一步研究水分对土壤锌有效性、 小麦生长、 产量以及锌和相关元素吸收利用的影响。【方法】田间试验于2010~2012年在陕西永寿县进行,采用裂区设计,锌肥为主处理,在不施锌与施锌(ZnSO4·7H2O)50 kg/hm2的基础上,设置在冬小麦关键生长期补充和不补充灌水2个副处理。在成熟期采集植株样品,测定了小麦产量、 生物量,各器官部位的锌及氮、 磷、 钾、 铁的含量; 采集0—40 cm土层土壤,测定了土壤有效性锌含量。【结果】在返青期、 孕穗期补灌20~30 mm水分对小麦产量、 土壤有效锌含量无显著影响,却有提高小麦各部位锌含量、 锌肥利用率的趋势,不施锌和施锌条件下,灌水比不灌水处理小麦籽粒锌含量分别提高3.8%~16.3%、 3.8%~13.1%,灌水使锌肥利用率提高21.2%~177.8%。灌水量和灌水时期的不同也影响锌在小麦各器官部位的分配与累积,第一季施锌和不施锌条件下,灌水比不灌水处理锌收获指数分别降低5.1%和2.0%,而第二季锌收获指数分别提高2.1%和2.7%。两季灌水对小麦籽粒中铁及大量元素氮磷钾含量的影响亦各不相同。【结论】在旱地缺锌土壤上,小麦生长关键期灌水对小麦产量、 土壤有效锌含量无显著影响,却有提高小麦各部分锌含量、 锌肥利用率的趋势,说明水肥结合对旱地石灰性土壤锌和锌肥有效性的影响应引起进一步重视,这对提高旱地缺锌地区作物和人体锌营养水平具有潜在意义。