Evaluation of nanosilicon dioxide and chitosan on tissue culture of apple under agar-induced osmotic stress

In vitro, applications of nanosilicon dioxide (SiO₂) and chitosan were investigated for their effects on growth and proliferation of apple (Malus domestica Borkh. ‘Gala’) explants under osmotic stress induced by agar to simulate drought stress and under non-stressed conditions. The experiment included five levels of SiO₂ (0, 25, 50, 100, and 200 mg L^?1), two levels of chitosan (0 and 40 mg L^?1), and two levels of agar (7 g L^?1 and 9 g L^?1) added to Murashige and Skoog medium. Under non-stressed conditions (7 g L^?1 agar), application of SiO₂ at 50 or 100 mg ^?1 increased proliferation of apple explants. Use of 50 or 100 mg L^?1 SiO₂ or 40 mg L^?1 chitosan increased growth of apple explants under osmotic stress (9 g L^?1 agar). This research suggests that use of SiO₂ or chitosan may improve plant growth and tolerance to stress.     

乙烯利对苹果矮化砧T337幼苗硝态氮吸收、分配和利用的影响

【目的】本试验探究叶片喷施乙烯利对苹果矮化砧T337幼苗15N-NO3-吸收利用和分配的影响,为苹果生产中氮肥的合理施用提供科学依据。【方法】供试材料为T337幼苗,进行水培试验。幼苗先在NO3-浓度为10mmol/L的改良Hoagland营养液中预处理32天,然后饥饿培养7天,进行乙烯利处理试验。试验设叶面喷施乙烯利200μL/L (E1)和400μL/L (E2) 2个浓度,以喷清水为对照(CK)。分别于喷施后第0、4、8、12、16、20天取样测定幼苗的根系活力,根部和叶片中硝酸还原酶活性及谷氨酰胺合成酶活性;于第20天(试验结束),取样分析幼苗15N吸收、分配及利用率。【结果】各处理苹果矮化砧T337幼苗根系活力随处理时间延长呈现先升后降的趋势,在第8天均达到最大值,不同处理间差异显著。6次取样检测结果,E1、E2处理的幼苗根系活力均明显高于CK,E2处理又高于E1。不同处理幼苗根和叶片中硝酸还原酶和谷氨酰胺合成酶活性均在处理后第8天达到高峰。E1、E2处理的两种酶活性在处理后12天内高于对照,16~20天低于对照。对照和E1处理的幼苗叶片中硝酸还原酶活性在20天内一直高于同时期的根系,而E2处理只在12天内叶片硝酸还原酶活性高于根系,16天后根系高于叶片。E1处理叶片干重明显高于对照,E2处理根系和叶片干重分别比对照增加87.0%和28.8%,幼苗根冠比也显著高于对照。乙烯利能够明显提高幼苗氮肥利用率,促进氮素向幼苗根系部位分配。E1和E2处理15N利用率分别比对照高出3.44个百分点和15.32个百分点,根系15N分配率比对照分别高33.32%、67.40%。【结论】叶面喷施乙烯利可以影响幼苗根系及叶片中硝酸还原酶和谷氨酰胺合成酶活性,增加T337幼苗的生物量,提高根冠比;并且可显著提高幼苗对硝态氮的利用率,增大15N在幼苗根系中的分配率,其中以400μL/L乙烯利的处理效果最好。     

Behavior of soil boron and boron uptake by M.26 apple rootstock as affected by application of different forms and nitrogen rates

Abstract

The changes in availability and uptake of boron (B) by M.26 apple rootstocks as affected by applications of different forms and rates of nitrogen (N) were examined. The study was carried out in a greenhouse using soil with low contents of organic matter, clay, calcium carbonate, NH₄‐oxalate soluble aluminum (Al) and iron (Fe), NH₂OH·HCl extractable manganese (Mn), poor cation exchange capacity and low pH. Soil N application was in the form of urea, calcium nitrate, ammonium sulphate, or ammonium nitrate at rates of 0, 17, 34, and 51 mg N kg^?1. After 1, 3, and 5 days of N application, soil B fractions were determined: B in soil solution, B specifically and non‐specifically adsorbed on soil surfaces, B occluded in Mn oxyhydroxides, and B occluded in crystalline Al and Fe oxides. The results showed that N as calcium nitrate and ammonium nitrate increased B both in soil solution and non‐specifically adsorbed on soil surface and decreased B concentration on Al and Fe oxides. This indicates that N‐NO₃ inhibited B sorption on Fe and Al oxides. Maximum B desorption from Fe and Al oxides was obtained within one day after N‐NO₃ was supplied. Nitrogen application as calcium nitrate and ammonium nitrate increased availability and uptake of B by plant roots. Thus, it was concluded that apple trees planted on coarse‐textured soils where risk of B deficiency is high, calcium nitrate or ammonium nitrates would be appropriately to apply to keep B more available.     

不同砧木对脐橙幼树生长和叶片养分含量变化的影响

以体细胞杂种红桔+枳和红桔+粗柠檬、有性杂种Troyer枳橙和Sweingel枳柚作砧木的脐橙2年生嫁接苗为试材,利用盆栽试验研究了不同砧木对苗木生长和叶片养分含量年动态变化的影响。结果表明,红桔+枳的生长势和花量明显地优于其它砧木,红桔+粗柠檬的生长和花量介于2种有性杂种之间;砧木基本上不影响叶片N、P、K、Mg和Mn含量年变化,但明显影响叶片Ca、Fe和Zn含量的年变化;在同一个生长期,不同砧木对叶片养分含量有明显的影响。此外本文还讨论了体细胞杂种砧木的利用价值。     

荸荠试管结球茎的试验研究

以两个荸荠品种（四塘荸荠和郴州荸荠）的试管苗为试验材料．进行不同因素对试管苗结球茎的试验。结果表明：（1）不同蔗糖浓度对荸荠试管结球茎有显著影响，当蔗糖浓度为60g／L时，两个品种的结球茎个数和平均鲜重都达到了最大值；（2）6-BA对荸荠试管结球茎的效果明显．其最佳浓度为3mg／L；（3）温度对荸荠试管结球茎也有重要影响，日夜温差大对试管结球茎效果好。     

Effects of excess boron on growth,gas exchange,and boron status of four orange scion–rootstock combinations

Field observations indicate that boron (B)‐toxicity symptoms may occur in citrus plants from inappropriate foliar spraying or overfertilizing with B especially under low‐rainfall conditions, where B can accumulate to levels that become toxic to plant growth. Previous work has indicated that different rootstocks can greatly influence the scion's tolerance to B toxicity, however, little is known about the response of different citrus scion–rootstock combinations to excess‐B conditions. In the present study, we investigated the effects of excess B on plant growth, gas exchange, B concentration, and distribution of four scion–rootstock combinations, Newhall and Skagg's Bonanza navel orange (Citrus sinensis Osb.) scions grafted on Carrizo citrange (C. sinensis L. Osb. × Poncirus trifoliata L. Raf.) and Trifoliate orange (Poncirus trifoliata L. Raf.) rootstocks. One‐year‐old plants of the four scion–rootstock combinations were grown for 183 d in sand–perlite (1 : 1, v/v) medium under greenhouse conditions. The plants were irrigated with half‐strength Hoagland's nutrient solution containing two B concentrations, 0.25 (control) and 2.50 (excess B) mg L^–1. It was found that, apart from the combination of Newhall grafted on Carrizo citrange, the dry weights in various parts of the other three combinations were reduced by the excess‐B treatment. Furthermore, the plants of Skagg's Bonanza grafted on Carrizo citrange showed the highest growth reduction amongst the four scion–rootstock combinations. In most cases, the greater reductions in dry weight were found in roots as compared to the other plant parts under excess‐B conditions, indicating that roots were more sensitive to B toxicity than the other tissues. In the case of Newhall plants grafted on Carrizo citrange, the entire plant growth was increased by excess‐B treatment. Boron concentrations in all plants parts increased significantly by increasing the B supply in the nutrient solution. Leaves were the dominant sites of B accumulation and showed the greatest increase in B concentration compared to the other plant parts, as B concentration in the nutrient solution increased. Our results indicate that the combination of Newhall grafted on Carrizo citrange was more tolerant to B toxicity, while the combination of Skagg's Bonanza grafted on Carrizo citrange was relatively more sensitive to B toxicity, in comparison with the other scion–rootstock combinations. However, Newhall plants contained more B in leaves and in roots than Skagg's Bonanza plants when they were both grafted on Carrizo citrange, indicating that the mechanism underlying such great differential growth responses of the two scion–rootstock combinations to B toxicity may not be associated with B exclusion from roots or reduced translocation of B to shoots. Furthermore, B distribution in different plant parts implied that the mechanism was also unlikely related to altered distributions of accumulated B in plant tissues. However, inherent ability to tolerate excessive B concentration in plant tissues may be involved in B tolerance.     

二氧化氯对苹果表面和鲜榨苹果汁中酿酒酵母1450的杀灭效果

利用二氧化氯(ClO₂)对水、苹果汁中和苹果表面的酿酒酵母1450(Saccharomyces cerevisiae)进行杀菌处理。结果表明：6、12 mg/L的ClO₂处理15 min使水中酵母分别减少(5.81±0.12)和(6.20±0.05)lg cfu/mL;100 mg/L的ClO₂对苹果汁中酵母的杀菌作用不明显,200 mg/L的ClO₂处理1 h可将果汁中(3.79±0.04)lg cfu/mL的酵母完全杀死,使果汁中(6.48±0.03)lg cfu/mL的酵母减少(3.67±0.05)lg cfu/mL,300 mg/L的ClO₂可将苹果汁中(6.52±0.06)lg cfu/mL的酵母完全杀死;10、20、30和40 mg/L的ClO₂处理1 h使苹果表面接种量为(4.95±0.02)lg cfu/mL的酵母数分别减少(3.41±0.02)、(3.64±0.08)、(3.80±0.04)、(4.47±0.09)lg cfu/mL,50 mg/L的ClO₂处理1 h,可将接种于苹果表面的酵母全部杀死,接种量进一步增加,ClO₂对苹果表面酵母的杀灭效果将会减弱;ClO₂处理对苹果汁的理化指标影响较小。     

组培苗光合速率测量系统的研制与试验

光合生长模型是组培微环境调控的依据，组培苗光合速率测量系统是定量研究生长模型所必备的实验装置。现有成熟的植物光合速率测量系统，如Li-6400不能适用于组培苗的测量。该文在综合分析国外现有测量系统的基础上，兼顾国内在大型组培育苗设施类型选择上的经济可行性，采用CO₂传感器和自动控制技术，研制了半开放式组培苗光合速率测量系统。该系统自动化程度高，能够实现整个生长过程在线连续测量，测量误差小，不干扰组培微环境，数据真实性好，所建立的光合模型可以直接应用于半开放式组培设施环境的调控系统。以阶段Ⅲ甘薯组培苗为实验材料，采用本测量系统对其第8 d的光合速率进行了测定，并建立了CO₂浓度和光合光量子通量密度的2因子光合生长模型。     

施氮水平对五种苹果砧木生长以及氮素吸收、分配和利用特性的影响

以一年生平邑甜茶（M. hupehensis Rehd.）、 八棱海棠（M. micromalus Makin）、 楸子［M. micromalus (Willd) Borkh.］、 新疆野苹果［M. micromalus (Ledeb.) Roemer］、 东北山荆子（M. baccata Borkh.）为试材,采用15N示踪技术,研究了施氮量对5种砧木生长及15N吸收、 分配和利用特性的影响。结果表明, 砧木的生物量、 根系总表面积、 总根长和根尖数均随施氮量的增加而增加,但不同砧木增加的程度不同,且地上部生长量对氮素的反应比地下部更敏感。平邑甜茶的生物量最大,楸子的根系总表面积、 总根长和根尖数均最大; 东北山荆子的生物量、 根系总表面积、 总根长和根尖数均最小。不同品种的15N利用率随施氮量增加变化不同且差异显著,并与根系总表面积存在显著正相关关系; 楸子的15N利用率最高,东北山荆子的最低。低氮处理（N1）中各器官从硝态氮中吸收分配到的15N量对该器官全氮量的贡献（Ndff）从大到小顺序为根叶茎,高氮处理（N2）中各器官的Ndff值从大到小顺序为叶根茎。不同砧木的Ndff随施氮量增加变化不同。各砧木15N均主要分配在叶中,其次是根,茎中最少。     

结球白菜带柄子叶外植体不定芽离体再生能力的遗传分析

解析控制植物不定芽离体再生能力的遗传因素,有助于从根本上提高离体培养困难材料的不定芽再生能力,是植物基因工程遗传改良的基础。本研究以结球白菜4个纯合亲本及其杂交后代的带柄子叶为外植体,建立了高频率离体不定芽再生技术。据此我们还对白菜不定芽再生能力进行遗传分析,并开展亲本与后代杂种的离体培养及反应研究,包括对培养基的激素需求,不定芽形成频率,不定芽形成数量,芽形态等系列特征。研究结果表明,杂种与亲本在培养基的激素浓度上有类似需求;杂交后代的不定芽再生率均至少高于一方亲本,在一些组合中可以高于双亲;在适宜的杂交组合及培养条件下,结球白菜带柄子叶外植体的不定芽再生率可以达100％,每一外植体上的不定芽数达3～7个。方差分析表明,结球白菜的不定芽离体再生能力存在基因的加性效应。     

砷污染土壤中接种丛枝菌根真菌和蚯蚓对土壤线虫群落和植物砷吸收的影响

The influences of arbuscular mycorrhizal fungi (MF, Acaulospora spp. and Glomus spp.), rice straw and earthworms (RE, Eisenia foetida) on nematode communities and arsenic (As) uptake by maize (Zea mays L.) in As-contaminated soils were examined in a field experiment conducted in Wujiang, Jiangsu Province, China. The experiment was designed as a 2 × 2 factorial with the factors of MF (inoculated or uninoculated) and RE (added or not added). The results demonstrated that MF inoculation led to significantly higher root colonization of MF and root dry weight. Plants inoculated with both MF and RE had the highest As concentrations in root. The number of total nematodes increased with MF inoculation when RE was absent, and decreased with RE addition when MF was inoculated. The improved abundance of nematodes with the MF treatment implied that the tested MF acted as food sources for fungivores. The abundances of omnivores-predators and plant parasites were reduced by earthworm activity. Twenty-seven genera of nematodes were identified, with Filenchus dominant in all treatments. Trophic diversity (TD), Shannon-Weaver diversity (H′), Simpson dominance index (λ), and species richness (SR) indicated higher species diversity, more proportionate species composition, evenly distributed species, and more food sources in the MF, RE, and their interaction treatments. Maturity index (MI) showed a moderately disturbed environment due to As pollution. Besides enhancing plant uptake of contaminants, MF and RE amendments could also improve soil health by restoring the structure of soil communities, as reflected by more stable nematode community structure.     

Influence of Ca concentration on micronutrient imbalances in in vitro propagated prunus rootstock

In vitro propagated plums of St. Julien GF 655–2 [Prunus insititia (L.)] (655–2), Damas GF 1869 [Prunus domestica (L.)] (D1869), and Clark Hill Redleaf [Prunus salicina (Until.) X Prunus cerasifera (Ehrh.)] (CHR), were grown in the greenhouse in nutrient solutions of 2, 6, 22, 66, 202, and 404 μM Ca for 96 days. 655–2 plants became severely chlorotic in Ca treatments of 66, 202, and 404 μM concentration after 86 days of growth. D1869 plants in 202 and 404 μM Ca exhibited slight interveinal chlorosis of new growth, while CHR exhibited no chlorosis at any Ca concentration. The best tissue nutrient indicator of chlorosis among rootstocks was foliar P/Fe and P/Zn ratios. 655–2 plants absorbed more P at higher Ca concentrations than did the other rootstock, resulting in the highest stem and leaf P/Fe, and P/Zn ratios. CHR plum may provide an easily propagated, chlorosis‐resistant rootstock for use on calcareous soils.     

不同氮形态对连作平邑甜茶幼苗生长及土壤尖孢镰孢菌数量的影响

【目的】研究盆栽条件下不同形态氮对苹果砧木—平邑甜茶幼苗生长以及对连作土壤微生物的影响,为连作建园时科学施肥、减轻苹果连作障碍提供依据。 【方法】试验于 2015 年露地进行,以平邑甜茶幼苗为试材,以连作土为对照1 (CK1) 和溴甲烷熏蒸为对照2 (CK2),设置施入铵态氮 (T1)、硝态氮 (T2) 和酰胺态氮 (T3) 3 个不同形态氮处理 (N 180 mg/L),于 2015 年 8 月测定平邑甜茶生物量、光合参数和土壤微生物等相关指标。 【结果】铵态氮 (T1) 和酰胺态氮 (T3) 处理均能明显提高连作条件下‘平邑甜茶’苹果幼苗的生物量,其中 T1 处理的株高、地径、鲜重、干重与 CK1 相比分别提高了 35.3%、24.4%、42.0%、57.7%。添加 3 种形态氮素均可不同程度地提高平邑甜茶幼苗叶片的净光合速率 (Pn)、气孔导度 (Gs) 和根系呼吸速率,其中 T1 处理的 Pn、Gs 和根系呼吸速率分别比 CK1 提高了 27.6%、35.6%、43.3%,却并未达到 CK2 的效果。实时荧光定量 PCR 结果表明,CK2 尖孢镰孢菌的基因拷贝数降低最为明显,比 CK1 降低了 52.6%,T1 也有效地降低了尖孢镰孢菌的基因拷贝数,而 T2 和 T3 处理尖孢镰孢菌的基因拷贝数与 CK1 没有显著性差异。T-RFLP 结果表明,T1 和 T3 处理的真菌群落结构与 CK1 有明显差异,T1 处理与 CK2 的真菌群落结构较为相似,T2 处理的真菌群落结构与 CK1 较为相似。 【结论】铵态氮处理可更好地提高连作平邑甜茶幼苗的生物量、净光合速率以及根系呼吸速率,降低土壤尖孢镰孢菌的基因拷贝数,明显改变连作土壤的真菌群落结构,可作为减轻苹果连作障碍的措施。     

滴灌施氮对苹果氮素吸收和利用的影响

以8年生嘎富苹果/八棱海棠（Malus robusta Rehd）为试材,研究了滴灌施肥下不同滴头数量对其滴施15N-尿素的吸收、分配与利用特性。结果表明:不同滴头数量处理,果实成熟期植株各器官Ndff%差异显著,DF2（两个滴头滴灌施肥处理）各器官Ndff%显著高于DF1（一个滴头滴灌施肥处理）和CK,DF1和CK差异不显著。3个处理均以果实的Ndff %值最高,分别为3.84%、3.14%和3.16%;新梢旺长期和果实膨大期DF2处理果实的Ndff%低于DF1和CK,但在果实成熟期Ndff %超过DF1和CK,DF1和CK 差异不显著。果实成熟期生殖器官分配率最高,营养器官和贮藏器官均较低,处理间差异不显著。DF2处理的15N利用率为38.95%,显著高于DF1（27.68%）和CK（23.69%）。随生长期的推移,各处理间020cm和2040cm土层硝态氮含量变化趋势一致,均呈双峰趋势,峰值分别出现在新梢旺长期和果实膨大期;6080cm和80100cm土层硝态氮含量变化趋势也一致,均变化较为平缓,而4060cm土层硝态氮含量变化差异显著,DF2处理明显高于DF1和CK。     

施硅对高锰诱发苹果粗皮病的影响

为解决辽宁等地高锰引起苹果生理粗皮病发生的问题,2004年在盆栽脱毒富士幼苗上进行施锰的同时施硅与单施锰的试验,待叶、枝发生粗皮病后再施硅矫正。结果表明,本试验条件下施锰400.mg/kg的同时施硅400mg/kg,可安全有效防治粗皮病的发生;施锰400.mg/kg诱发叶片发病后,施硅400.mg/kg可较好地控制粗皮病的发生。施锰400.mg/kg枝干发病后施硅,对粗皮病的蔓延有一定控制作用,但效果不明显。随着施硅量增加,土壤速效钾含量提高,有效铁、有效锌含量降低,说明粗皮病的发生也与上述养分有关系。     

仔猪睾丸组织块生精细胞体外培养及鉴定初报

目的：建立仔猪睾丸组织块生精细胞爬片体外培养模型。方法：应用溴脱氧核苷尿嘧啶(5＇-Bromo-2＇-deoxyuridine, BrdU)免疫荧光技术检测生精细胞体外增殖分化情况；采用HE染色鉴定体外培养生精细胞分化程度。结果：结果表明体外培养第3天支持细胞开始游出睾丸组织块并贴壁；体外培养第4～8天睾丸组织块周围可见生精细胞，但未见明显增殖分化情况；体外培养第9～20天可明显观察到生精细胞增殖分化，间桥连接(gap junction)的生精细胞集落呈葡萄串或串珠状，并有精子细胞形成。结论：本培养体系能够提供睾丸组织块生精细胞增殖分化所需要的条件，在没有添加睾酮的情况下能够使非精子细胞分化为精子细胞。     

种植苹果树对渭北果园土壤胶结物质分布的影响

本研究通过系统研究种植果树对土壤胶结性物质的演化规律及其与土壤团聚体稳定性之间关系的影响,探索影响果园土壤团聚体状态的因素,以期为果园科学管理提供理论依据。在渭北旱塬苹果主产区分别选取10 a、20 a的苹果园和农田(冬小麦-夏玉米轮作,对照)各4个,在果树冠层投影范围内距树干2/3处逐层采集0~100 cm土层土壤样品和0~50 cm土层原状土壤样品,研究不同植果年限果园及农田土壤剖面黏粒、有机质、CaCO_3等团聚体胶结物质的分布及其与团聚体稳定性之间的关系。结果发现:在0~100 cm土层范围内,各果园土壤黏粒含量基本随土层深度的增加而递增,且在0~40 cm土层表现为农田10 a果园20 a果园,40 cm以下土层则呈现相反的态势;种植果树相比农田可显著增加0~100 cm土层土壤有机质总储量,但随着种植果树年限的增加,土壤有机质总储量呈递减趋势;在0~100 cm土层土壤CaCO_3总储量表现为10 a果园农田20a果园,但在0~40 cm土层CaCO_3含量及储量表现为10 a果园农田20 a果园,而40~100 cm土层则为20 a果园10 a农田。皮尔森相关分析发现(29)0.25 mm土壤团聚体的数量和平均重量直径(MWD)与土壤黏粒、有机质和CaCO_3含量密切相关,其中机械稳定性团聚体的数量和稳定性主要受土壤中CaCO_3、有机质含量的影响,水稳性团聚体的数量和稳定性主要受土壤中黏粒和CaCO_3的影响。总之,植果显著改变了土壤中黏粒、有机质、CaCO_3的演化过程和趋势,随植果年限增加,果园土壤黏粒和CaCO_3在土壤较深土层淋溶淀积明显;各果园土壤有机质总储量虽然高于农田,但随植果年限增加,有逐渐减少的趋势。可见植果明显加速了渭北黄土塬地土壤的残积黏化和钙化过程,影响着表层土壤团聚作用和底层土壤的紧实化和坚硬化程度。     

肌醇与硝酸银对霍霍巴多芽苗增殖的促进作用简报

试验研究结果表明在分化培养基中添加适当浓度的肌醇,有利于霍霍巴试管苗的生长和分化,其叶面积明显增大,芽分化率提高;1～3 5mg/ L浓度硝酸银对霍霍巴多芽增殖有显著促进作用,但对株高的影响不显著,且硝酸银浓度过高对试管苗有一定抑制作用,其最适宜浓度为3mg/ L。若二者同时添加效果更显著,其增殖系数可达10 9。     

A Preliminary Study on Physiological and Molecular Effects of Iron Deficiency in Fuji/Chistock 1

In order to get experimental data on apple rootstock with iron-efficient genotypes capable of improving scion resistance to iron deficiency, this experiment was conducted on the physiological and molecular characteristics of Fuji/ Chistock 1 (F/C) under different iron conditions and compared it to Fuji/ M. Baccata (F/B). F/C was less sensitive to iron deficiency than F/B. F/B showed chlorosis after 25 days under iron-deficient conditions, but F/C showed no phenotypic changes, even after 40 days. The shoot and leaf area growth of F/C were respectively 5cm and 1000 mm² higher than those of F/B, regardless of the iron-deficient or iron-efficient conditions. The young leaf chlorophyll and active iron of F/C were 5 SPAD and 5 mg kg^?1 higher than those of F/B, either in iron-deficient or iron-efficient conditions. The expression of YSL5 and CS1 showed the same pattern. The enhancement expression of iron transport genes may be one explanation for these findings.     

Effects of Grafting on Alkali Stress in Tomato Plants: Datura Rootstock Improve Alkalinity Tolerance of Tomato Plants

Plant growth, nutritional status, and proline content were investigated in non-grafted and grafted greenhouse tomato plants onto five rootstocks of eggplant, datura, orange nightshade, local Iranian tobacco, and field tomato, exposed to 0, 5, and 10 mM sodium bicarbonate (NaHCO₃) to determine whether grafting could improve alkalinity tolerance of tomato. The leaf fresh mass of ungrafted and grafted tomato plants decreased significantly as NaHCO₃ levels increased. Despite other rootstocks and ungrafted plants, alkalinity had no significant effect on stem and root fresh mass and shoot phosphorus (P), potassium (K) and magnesium (Mg) concentrations of datura grafted plants. The lowest solution pH and electrical conductivity (EC) values and the highest leaf proline content were observed in the plants grafted onto datura rootstock. Moreover, sodium (Na) concentration in shoots was lower in plants grafted onto datura rootstock than in other plants especially under high NaHCO₃ levels. Overall, using datura rootstock improved alkalinity tolerance of tomato plants under NaHCO₃ stress.