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Salmonella enterica serovar enteritidis burden in broiler breeder chicks genetically associated with vaccine antibody response 总被引:3,自引:0,他引:3
The relationship between antibody response to Salmonella enteritidis vaccine and internal organ burden of S. enteritidis is not fully understood. The genetic relationship, therefore, between postchallenge S. enteritidis burden and antibody response to S. enteritidis vaccine was determined in broiler breeder chicks. Sibling chicks from a broiler breeder male line were either inoculated with a pathogenic S. enteritidis or vaccinated with a commercial S. enteritidis vaccine. Spleen, liver, cecal wall, and cecal content samples from S. enteritidis-challenged chicks (n = 120) were cultured for enumeration of bacteria. Unchallenged chicks (n = 314) were vaccinated at 11 days of age, and serum samples were taken at 10 days postvaccination. Antibody response to vaccination and number of S. enteritidis in cecal content cultures were negatively correlated (-0.772), demonstrating that genetic potential for greater antibody response to S. enteritidis vaccine is associated with lesser S. enteritidis bacterial burden in cecal content of broiler breeder chicks. The findings suggest that genetic selection for vaccine antibody responsiveness can lower bacterial burden in the gut lumenal content and, thus, potentially reduce contamination of poultry products at processing. 相似文献
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为了建立零售鸡肉中沙门氏菌的生长预测模型,以在超市购买的新鲜鸡肉和肠炎沙门氏菌作为研究对象,选择4、10、16、25、30、37℃条件,对肠炎沙门氏菌在零售鸡肉中的生长情况进行研究,绘制生长曲线。对一级模型,用Curve Expert软件中的Gompertz、Richards、Logistic 3种模型和DMFit软件中的Baranyi模型进行拟合,以确定最适用模型。4种模型拟合后的Baranyi模型相关系数都在0.98以上。数据表明该模型拟合程度最好,最适合预测沙门氏菌在鸡肉中的生长动态。二级模型是将一级模型拟合的数据带入Ratkowsky方程。此方程描述的是温度对最大生长速率的影响。通过准确因子、偏差因子以及均方根误差,对模型的准确性进行检验。结果显示模型的准确因子为1.129 698,偏差因子为0.984 85,均方根误差为0.091 5,决定系数R2为0.982 5,说明建立的模型可靠性高。 相似文献
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1 作为人类胃肠疾病病因的沙门菌
世界范围的蛋相关沙门菌传染病始于1970年代,至目前已逐渐消失,这归功于政策制定者和家禽业的巨大努力.这种传染病是由肠炎型沙门菌引起的.由于其与鸡蛋关系密切,加之人们常在室温下储存、加工及食用生鸡蛋的习惯方式,肠炎型沙门菌对人类健康产生了重要影响.近年来,全欧盟的沙门菌污染水平不断下降,而沙门菌分离菌株对抗菌药的抗药性依然不断上升.尤其是鼠伤寒沙门菌格外引人关注,因为在2006年大约40%的鼠伤寒沙门菌分离菌株对4种以上的抗菌药耐药. 相似文献
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《畜牧与生物技术杂志(英文版)》2019,(4)
Background: Increasing evidence indicates that micro RNAs(mi RNAs) are involved in inflammatory response and immune regulation following pathogen invasion. The purpose of this study was to elucidate the roles played by Gallus gallus micro RNA-1306-5 p(gga-mi R-1306-5 p) in host responses against potential invasion by Salmonella enteritidis(SE) in chickens and the underlying mechanisms.Results: In present study, the expression levels of gga-mi R-1306-5 p were determined in both tissues and HD11 cells. The results showed that gga-mi R-1306-5 p was significantly increased following SE infection or lipopolysaccharide(LPS) stimulation. The dual luciferase reporter assay further validated that gga-mi R-1306-5 p targeted the Toll-interacting protein(Tollip), and thereby participated in the regulation of immune response against SE or LPS stimulation through binding with the 3′-untranslated region(3'UTR) of Tollip. Additionally, the expression of Tollip was significantly blocked by over-expressed gga-mi R-1306-5 p. The underlying mechanisms by which ggami R-1306-5 p modulated the production of pro-inflammatory cytokines were also investigated. Molecular biological assays demonstrated that overexpression of gga-mi R-1306-5 p promoted the production of pro-inflammatory mediators, including NF-κB, TNF-α, IL-6, and IL-1β, which produced effects similar to those of Tollip knockdown.Conclusions: Taken together, gga-mi R-1306-5 p induced by SE or LPS, regulates the immune response by inhibiting Tollip, which activates the production of inflammatory cytokines. This study has provided the first direct evidence that gga-mi R-1306-5 p targets Tollip, and is involved in the host response against SE. 相似文献
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L. Vrbova S. Sivanantharajah R. Walton Y. Whitfield C. Lee I. Picard N. Chapinal C. Gaulin L. Tschetter J. Tataryn 《Zoonoses and public health》2018,65(4):386-394
In December 2012, an increase in human Salmonella Typhimurium cases was identified in the province of Ontario, Canada launching an outbreak investigation. The outbreak spanned 3 years (2012–2014), with 134 cases reported from five Canadian provinces. There was a substantial burden of illness among children: 45% of cases were children 12 years old or under, and 23% of cases were under 5 years old. Epidemiologic, traceback and laboratory findings linked this outbreak to feeder rodents (used to feed snakes) supplied by a network of rodent breeders in Ontario. Cases likely acquired their illness through either direct or indirect contact with feeder rodents. This investigation not only contributes to the weight of evidence on the risk that feeder rodents pose, but also underscores the importance of investigating indirect animal contact and associated risks, especially for high‐risk individuals. 相似文献
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Salmonella typhimurium was isolated from the peritoneal exudate of a kitten which died from an acute peritonitis.
Résumé. On a isolé Salmonella typhimurium de l'exudat péritonéal d'un chaton, mort d'une péritonite aiguë.
Zusammenfassung. Salmonella typhimurium wurde aus dem peritonealen Exsudat einer jungen Katze isoliert, die an einer akuten Peritonitis starb. 相似文献
Résumé. On a isolé Salmonella typhimurium de l'exudat péritonéal d'un chaton, mort d'une péritonite aiguë.
Zusammenfassung. Salmonella typhimurium wurde aus dem peritonealen Exsudat einer jungen Katze isoliert, die an einer akuten Peritonitis starb. 相似文献
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Nadia Bergeron Jonathan Corriveau Ann Letellier France Daigle Sylvain Quessy 《Canadian journal of veterinary research》2010,74(1):11-17
Salmonella Typhimurium is frequently isolated from pigs and may also cause enteric disease in humans. In this study, 33 isolates of S. Typhimurium associated with septicemia in swine (CS) were compared to 33 isolates recovered from healthy animals at slaughter (WCS). The isolates were characterized using phenotyping and genotyping methods. For each isolate, the phage type, antimicrobial resistance, and pulsed-field gel electrophoresis (PFGE) DNA profiles were determined. In addition, the protein profiles of each isolate grown in different conditions were studied by Coomassie Blue-stained sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot. Various phage types were identified. The phage type PT 104 represented 36.4% of all isolates from septicemic pigs. Resistance to as many as 12 antimicrobial agents, including some natural resistances, was found in isolates from CS and WCS. Many genetic profiles were identified among the PT 104 phage types. Although it was not possible to associate one particular protein with septicemic isolates, several highly immunogenic proteins, present in all virulent isolates and in most isolates from clinically healthy animals, were identified. These results indicated that strains associated with septicemia belong to various genetic lineages that can also be recovered from asymptomatic animals at the time of slaughter. 相似文献
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为了解昆明周边养鸡场的沙门氏菌耐药状况,采集昆明周边5家养殖场的50只疑似沙门氏菌病的发病鸡的肝脏和脾脏进行细菌分离,通过常规生化特性鉴定和PCR分子鉴定,然后通过药敏试验检测分离株耐药性,通过PCR技术对分离株的耐药基因进行检测。结果表明:共分离出8株沙门氏菌,8株菌的靛基质试验、尿素酶试验和VP试验结果均呈阴性,S3、S4菌株不产H2S,柠檬酸试验也呈阴性,针对沙门氏菌设计特异性引物进行扩增,得到目的条带495 bp,与预期一致;在药敏试验中,分离株对氨苄西林和头孢氨苄有较强的耐药性,对四环素中介耐药,对恩诺沙星、加替沙星、卡那霉素、庆大霉素以及氧氟沙星敏感,说明分离株已出现耐药性;在所检测的6种耐药基因中,β-内酰胺类的bla TEM-1和喹诺酮类的GyrA基因检出率为100%,四环素类的tetA基因未被检出,其他三种耐药基因aadA1、tetB和strB检出率均在50%以上,说明分离株耐药基因的携带率较高,需要引起养殖业的重视。 相似文献
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用从健康鸡消化道分离的鸡源乳酸杆菌4株,双歧杆菌2株,对引起雏鸡腹泻的鸡白痢沙门氏菌和鸡副伤寒沙门氏菌进行体外生物拮抗试验。试验结果显示:3株乳酸杆菌、2株双歧杆菌、1株芽孢杆菌对致病性沙门氏菌均有明显的生物拮抗作用(P<0.01),而1株乳酸杆菌和1株芽孢杆菌拮抗作用不明显。 相似文献
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